"Ligase Chain Reaction" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Concept/Terms
Ligase Chain Reaction- Ligase Chain Reaction
- Chain Reaction, Ligase
- Chain Reactions, Ligase
- Ligase Chain Reactions
- Reaction, Ligase Chain
- Reactions, Ligase Chain
Below are MeSH descriptors whose meaning is more general than "Ligase Chain Reaction".
Below are MeSH descriptors whose meaning is more specific than "Ligase Chain Reaction".
This graph shows the total number of publications written about "Ligase Chain Reaction" by people in Harvard Catalyst Profiles by year, and whether "Ligase Chain Reaction" was a major or minor topic of these publication.
To see the data from this visualization as text,
click here.
| Year | Major Topic | Minor Topic | Total |
|---|
| 2003 | 0 | 2 | 2 |
| 2008 | 1 | 0 | 1 |
| 2009 | 1 | 1 | 2 |
| 2010 | 1 | 2 | 3 |
Below are the most recent publications written about "Ligase Chain Reaction" by people in Profiles.
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Ligation with nucleic acid sequence-based amplification. J Mol Diagn. 2012 May-Jun; 14(3):206-13.
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Design and generation of MLPA probe sets for combined copy number and small-mutation analysis of human genes: EGFR as an example. ScientificWorldJournal. 2010 Oct 12; 10:2003-18.
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Association between detection of HIV-1 DNA resistance mutations by a sensitive assay at initiation of antiretroviral therapy and virologic failure. Clin Infect Dis. 2010 May 15; 50(10):1397-404.
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Efficacy and safety of 1-month postpartum zidovudine-didanosine to prevent HIV-resistance mutations after intrapartum single-dose nevirapine. Clin Infect Dis. 2010 Mar 15; 50(6):898-908.
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Detection of HIV-1 drug resistance in women following administration of a single dose of nevirapine: comparison of plasma RNA to cellular DNA by consensus sequencing and by oligonucleotide ligation assay. J Clin Microbiol. 2010 May; 48(5):1555-61.
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Methylation-specific multiplex-ligation-dependent probe amplification as a rapid molecular diagnostic tool for pseudohypoparathyroidism type 1b. Genet Test Mol Biomarkers. 2010 Feb; 14(1):135-9.
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Screening of autosomal gene deletions in patients with hypogonadotropic hypogonadism using multiplex ligation-dependent probe amplification: detection of a hemizygosis for the fibroblast growth factor receptor 1. Clin Endocrinol (Oxf). 2010 Mar; 72(3):371-6.
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Designing a simple multiplex ligation-dependent probe amplification (MLPA) assay for rapid detection of copy number variants in the genome. J Genet Genomics. 2009 Apr; 36(4):257-65.
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New applications and developments in the use of multiplex ligation-dependent probe amplification. Electrophoresis. 2008 Dec; 29(23):4627-36.
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Global identification of Myc target genes reveals its direct role in mitochondrial biogenesis and its E-box usage in vivo. PLoS One. 2008 Mar 12; 3(3):e1798.