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Last Name

profileEdward A. Greenfield, Ph.D.

TitleResearch Associate in Medicine
InstitutionDana-Farber Cancer Institute
AddressDana-Farber Cancer Institute
Rm 409
21-27 Burlington Ave.
Boston MA 02215
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DFCI Monoclonal Antibody Core - Core services (5)

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  1. Greenfield EA. Sampling and Preparation of Mouse and Rat Serum. Cold Spring Harb Protoc. 2017 Nov 01; 2017(11):pdb.prot100271. PMID: 29093207.
    View in: PubMed
  2. Greenfield EA. Harvesting Tissue from Immunized Mice, Rats, and Hamsters. Cold Spring Harb Protoc. 2017 Nov 01; 2017(11):pdb.prot100362. PMID: 29093208.
    View in: PubMed
  3. Mahoney KM, Sun H, Liao X, Hua P, Callea M, Greenfield EA, Hodi FS, Sharpe AH, Signoretti S, Rodig SJ, Freeman GJ. PD-L1 Antibodies to Its Cytoplasmic Domain Most Clearly Delineate Cell Membranes in Immunohistochemical Staining of Tumor Cells. Cancer Immunol Res. 2015 Dec; 3(12):1308-15. PMID: 26546452; PMCID: PMC4743889 [Available on 12/01/16].
  4. Greenfield E, Griner E. Registered report: Widespread potential for growth factor-driven resistance to anticancer kinase inhibitors. Elife. 2014 Dec 10; 3. PMID: 25490934; PMCID: PMC4270159.
  5. Jones RT, Abedalthagafi MS, Brahmandam M, Greenfield EA, Hoang MP, Louis DN, Hornick JL, Santagata S. Cross-reactivity of the BRAF VE1 antibody with epitopes in axonemal dyneins leads to staining of cilia. Mod Pathol. 2015 Apr; 28(4):596-606. PMID: 25412847.
    View in: PubMed
  6. Rangachari M, Zhu C, Sakuishi K, Xiao S, Karman J, Chen A, Angin M, Wakeham A, Greenfield EA, Sobel RA, Okada H, McKinnon PJ, Mak TW, Addo MM, Anderson AC, Kuchroo VK. Bat3 promotes T cell responses and autoimmunity by repressing Tim-3–mediated cell death and exhaustion. Nat Med. 2012 Sep; 18(9):1394-400. PMID: 22863785; PMCID: PMC3491118.
  7. Lu X, Rios HF, Jiang B, Xing L, Kadlcek R, Greenfield EM, Luo G, Feng JQ. A new osteopetrosis mutant mouse strain (ntl) with odontoma-like proliferations and lack of tooth roots. Eur J Oral Sci. 2009 Dec; 117(6):625-35. PMID: 20121924; PMCID: PMC4066957.
  8. Hastings WD, Anderson DE, Kassam N, Koguchi K, Greenfield EA, Kent SC, Zheng XX, Strom TB, Hafler DA, Kuchroo VK. TIM-3 is expressed on activated human CD4+ T cells and regulates Th1 and Th17 cytokines. Eur J Immunol. 2009 Sep; 39(9):2492-501. PMID: 19676072; PMCID: PMC2759376.
  9. Beidelschies MA, Huang H, McMullen MR, Smith MV, Islam AS, Goldberg VM, Chen X, Nagy LE, Greenfield EM. Stimulation of macrophage TNFalpha production by orthopaedic wear particles requires activation of the ERK1/2/Egr-1 and NF-kappaB pathways but is independent of p38 and JNK. J Cell Physiol. 2008 Dec; 217(3):652-66. PMID: 18651635; PMCID: PMC2597272.
  10. Exley MA, Hou R, Shaulov A, Tonti E, Dellabona P, Casorati G, Akbari O, Akman HO, Greenfield EA, Gumperz JE, Boyson JE, Balk SP, Wilson SB. Selective activation, expansion, and monitoring of human iNKT cells with a monoclonal antibody specific for the TCR alpha-chain CDR3 loop. Eur J Immunol. 2008 Jun; 38(6):1756-66. PMID: 18493987; PMCID: PMC2864538.
  11. Strandberg L, Ambrosi A, Espinosa A, Ottosson L, Eloranta ML, Zhou W, Elfving A, Greenfield E, Kuchroo VK, Wahren-Herlenius M. Interferon-alpha induces up-regulation and nuclear translocation of the Ro52 autoantigen as detected by a panel of novel Ro52-specific monoclonal antibodies. J Clin Immunol. 2008 May; 28(3):220-31. PMID: 18071879.
    View in: PubMed
  12. Rodriguez-Manzanet R, Meyers JH, Balasubramanian S, Slavik J, Kassam N, Dardalhon V, Greenfield EA, Anderson AC, Sobel RA, Hafler DA, Strom TB, Kuchroo VK. TIM-4 expressed on APCs induces T cell expansion and survival. J Immunol. 2008 Apr 01; 180(7):4706-13. PMID: 18354194; PMCID: PMC2948965.
  13. Cai G, Anumanthan A, Brown JA, Greenfield EA, Zhu B, Freeman GJ. CD160 inhibits activation of human CD4+ T cells through interaction with herpesvirus entry mediator. Nat Immunol. 2008 Feb; 9(2):176-85. PMID: 18193050.
    View in: PubMed
  14. Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Bone. 2007 May; 40(5):1276-83. PMID: 17236833; PMCID: PMC1930165.
  15. Fan X, Rahnert JA, Murphy TC, Nanes MS, Greenfield EM, Rubin J. Response to mechanical strain in an immortalized pre-osteoblast cell is dependent on ERK1/2. J Cell Physiol. 2006 May; 207(2):454-60. PMID: 16419041.
    View in: PubMed
  16. Greenfield EA, Reddy J, Lees A, Dyer CA, Koul O, Nguyen K, Bell S, Kassam N, Hinojoza J, Eaton MJ, Lees MB, Kuchroo VK, Sobel RA. Monoclonal antibodies to distinct regions of human myelin proteolipid protein simultaneously recognize central nervous system myelin and neurons of many vertebrate species. J Neurosci Res. 2006 Feb 15; 83(3):415-31. PMID: 16416423.
    View in: PubMed
  17. Bi Y, Nielsen KL, Kilts TM, Yoon A, A Karsdal M, Wimer HF, Greenfield EM, Heegaard AM, Young MF. Biglycan deficiency increases osteoclast differentiation and activity due to defective osteoblasts. Bone. 2006 Jun; 38(6):778-86. PMID: 16364709.
    View in: PubMed
  18. Dai JC, He P, Chen X, Greenfield EM. TNFalpha and PTH utilize distinct mechanisms to induce IL-6 and RANKL expression with markedly different kinetics. Bone. 2006 Apr; 38(4):509-20. PMID: 16316790.
    View in: PubMed
  19. Rubin J, Murphy TC, Rahnert J, Song H, Nanes MS, Greenfield EM, Jo H, Fan X. Mechanical inhibition of RANKL expression is regulated by H-Ras-GTPase. J Biol Chem. 2006 Jan 20; 281(3):1412-8. PMID: 16306046.
    View in: PubMed
  20. Dardalhon V, Schubart AS, Reddy J, Meyers JH, Monney L, Sabatos CA, Ahuja R, Nguyen K, Freeman GJ, Greenfield EA, Sobel RA, Kuchroo VK. CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. J Immunol. 2005 Aug 01; 175(3):1558-65. PMID: 16034094.
    View in: PubMed
  21. Chen X, Dai JC, Orellana SA, Greenfield EM. Endogenous protein kinase inhibitor gamma terminates immediate-early gene expression induced by cAMP-dependent protein kinase (PKA) signaling: termination depends on PKA inactivation rather than PKA export from the nucleus. J Biol Chem. 2005 Jan 28; 280(4):2700-7. PMID: 15557275.
    View in: PubMed
  22. Rodig N, Ryan T, Allen JA, Pang H, Grabie N, Chernova T, Greenfield EA, Liang SC, Sharpe AH, Lichtman AH, Freeman GJ. Endothelial expression of PD-L1 and PD-L2 down-regulates CD8+ T cell activation and cytolysis. Eur J Immunol. 2003 Nov; 33(11):3117-26. PMID: 14579280.
    View in: PubMed
  23. Schmollinger JC, Vonderheide RH, Hoar KM, Maecker B, Schultze JL, Hodi FS, Soiffer RJ, Jung K, Kuroda MJ, Letvin NL, Greenfield EA, Mihm M, Kutok JL, Dranoff G. Melanoma inhibitor of apoptosis protein (ML-IAP) is a target for immune-mediated tumor destruction. Proc Natl Acad Sci U S A. 2003 Mar 18; 100(6):3398-403. PMID: 12626761; PMCID: PMC152304.
  24. Brown JA, Dorfman DM, Ma FR, Sullivan EL, Munoz O, Wood CR, Greenfield EA, Freeman GJ. Blockade of programmed death-1 ligands on dendritic cells enhances T cell activation and cytokine production. J Immunol. 2003 Feb 01; 170(3):1257-66. PMID: 12538684.
    View in: PubMed
  25. Meyers JH, Ryu A, Monney L, Nguyen K, Greenfield EA, Freeman GJ, Kuchroo VK. Cutting edge: CD94/NKG2 is expressed on Th1 but not Th2 cells and costimulates Th1 effector functions. J Immunol. 2002 Nov 15; 169(10):5382-6. PMID: 12421909.
    View in: PubMed
  26. Howie D, Okamoto S, Rietdijk S, Clarke K, Wang N, Gullo C, Bruggeman JP, Manning S, Coyle AJ, Greenfield E, Kuchroo V, Terhorst C. The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production. Blood. 2002 Oct 15; 100(8):2899-907. PMID: 12351401.
    View in: PubMed
  27. Ragab AA, Nalepka JL, Bi Y, Greenfield EM. Cytokines synergistically induce osteoclast differentiation: support by immortalized or normal calvarial cells. Am J Physiol Cell Physiol. 2002 Sep; 283(3):C679-87. PMID: 12176725.
    View in: PubMed
  28. Akbari O, Freeman GJ, Meyer EH, Greenfield EA, Chang TT, Sharpe AH, Berry G, DeKruyff RH, Umetsu DT. Antigen-specific regulatory T cells develop via the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity. Nat Med. 2002 Sep; 8(9):1024-32. PMID: 12145647.
    View in: PubMed
  29. Eppihimer MJ, Gunn J, Freeman GJ, Greenfield EA, Chernova T, Erickson J, Leonard JP. Expression and regulation of the PD-L1 immunoinhibitory molecule on microvascular endothelial cells. Microcirculation. 2002 Apr; 9(2):133-45. PMID: 11932780; PMCID: PMC3740166.
  30. Monney L, Sabatos CA, Gaglia JL, Ryu A, Waldner H, Chernova T, Manning S, Greenfield EA, Coyle AJ, Sobel RA, Freeman GJ, Kuchroo VK. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature. 2002 Jan 31; 415(6871):536-41. PMID: 11823861.
    View in: PubMed
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