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Edward A. Greenfield, Ph.D.

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Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Greenfield EA, DeCaprio J, Brahmandam M. Sarkosyl Preparation of Antigens from Bacterial Inclusion Bodies. Cold Spring Harb Protoc. 2020 Sep 01; 2020(9):pdb.prot100032. PMID: 32873728.
    Citations:    
  2. Greenfield EA. Standard Immunization of Rabbits. Cold Spring Harb Protoc. 2020 Sep 01; 2020(9):pdb.prot100305. PMID: 32873729.
    Citations:    
  3. Greenfield EA, DeCaprio J, Brahmandam M. Preparing GST-, His-, or MBP-Fusion Proteins from Bacteria. Cold Spring Harb Protoc. 2020 Sep 01; 2020(9):pdb.prot100024. PMID: 32873727.
    Citations:    
  4. Greenfield EA. Repetitive Immunization at Multiple Sites (RIMMS) of Mice, Rats, and Hamsters. Cold Spring Harb Protoc. 2020 05 01; 2020(5):100313. PMID: 32358052.
    Citations:    
  5. Greenfield EA. Subtractive Immunization for Mice, Rats, and Hamsters. Cold Spring Harb Protoc. 2020 05 01; 2020(5):100321. PMID: 32358053.
    Citations:    
  6. Greenfield EA. Standard Immunization of Mice, Rats, and Hamsters. Cold Spring Harb Protoc. 2020 03 02; 2020(3):100297. PMID: 32123014.
    Citations:    
  7. Greenfield EA. Immunizing Mice and Rats with Nitrocellulose-Bound Antigen. Cold Spring Harb Protoc. 2019 12 02; 2019(12). PMID: 31792142.
    Citations:    
  8. Greenfield EA, DeCaprio J, Brahmandam M. Preparing Protein Antigens from Sodium Dodecyl Sulfate-Polyacrylamide Gels for Immunization. Cold Spring Harb Protoc. 2019 12 02; 2019(12). PMID: 31792141.
    Citations:    
  9. Greenfield EA, DeCaprio J, Brahmandam M. Detecting Protein Antigens in Sodium Dodecyl Sulfate-Polyacrylamide Gels. Cold Spring Harb Protoc. 2019 12 02; 2019(12). PMID: 31792140.
    Citations:    
  10. Greenfield EA. Preparing Feeder Cell Cultures to Support Hybridoma Growth. Cold Spring Harb Protoc. 2019 11 01; 2019(11). PMID: 31676580.
    Citations:    
  11. Greenfield EA. Single-Cell Cloning of Hybridoma Cells by Limiting Dilution. Cold Spring Harb Protoc. 2019 11 01; 2019(11). PMID: 31676581.
    Citations:    
  12. Greenfield EA. Single-Cell Cloning of Hybridoma Cells by Growth in Soft Agar. Cold Spring Harb Protoc. 2019 11 01; 2019(11). PMID: 31676582.
    Citations:    
  13. Greenfield EA. Electro Cell Fusion for Hybridoma Production. Cold Spring Harb Protoc. 2019 10 01; 2019(10). PMID: 31575798.
    Citations:    
  14. Greenfield EA. Myeloma and Hybridoma Cell Counts and Viability Checks. Cold Spring Harb Protoc. 2019 10 01; 2019(10). PMID: 31575799.
    Citations:    
  15. Greenfield EA. Routes of Antigen Injection in Mice and Rats. Cold Spring Harb Protoc. 2019 06 03; 2019(6). PMID: 31160388.
    Citations:    
  16. Greenfield EA. Administering Anesthesia to Mice, Rats, and Hamsters. Cold Spring Harb Protoc. 2019 06 03; 2019(6). PMID: 31160387.
    Citations:    
  17. Greenfield EA, DeCaprio J, Brahmandam M. Transfected Dendritic Cell Immunizations. Cold Spring Harb Protoc. 2019 03 01; 2019(3). PMID: 30824623.
    Citations:    
  18. Greenfield EA. Preparing and Using Adjuvants. Cold Spring Harb Protoc. 2019 01 02; 2019(1). PMID: 30602559.
    Citations:    Fields:    
  19. Greenfield EA. Sampling and Preparation of Rabbit Serum. Cold Spring Harb Protoc. 2018 12 03; 2018(12). PMID: 30510130.
    Citations:    Fields:    
  20. Greenfield EA, DeCaprio J, Brahmandam M. Preparing Antigens Using a Baculovirus Expression System. Cold Spring Harb Protoc. 2018 12 03; 2018(12). PMID: 30510129.
    Citations:    Fields:    
  21. Greenfield EA, DeCaprio J, Brahmandam M. Making Weak Antigens Strong: Cross-Linking Peptides to KLH with Maleimide. Cold Spring Harb Protoc. 2018 10 01; 2018(10). PMID: 30275080.
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  22. Greenfield EA. Routes of Antigen Injection in Rabbits. Cold Spring Harb Protoc. 2018 09 04; 2018(9). PMID: 30181227.
    Citations:    Fields:    
  23. Greenfield EA. Administering Anesthesia to Rabbits. Cold Spring Harb Protoc. 2018 09 04; 2018(9). PMID: 30181226.
    Citations:    Fields:    
  24. Greenfield EA. Protein Quantitation. Cold Spring Harb Protoc. 2018 06 01; 2018(6). PMID: 29858339.
    Citations:    Fields:    
  25. Greenfield EA, DeCaprio J, Brahmandam M. Making Weak Antigens Strong: Modifying Antigens by Dinitrophenol or Arsynyl Coupling. Cold Spring Harb Protoc. 2018 05 01; 2018(5). PMID: 29717054.
    Citations:    Fields:    
  26. Greenfield EA, DeCaprio J, Brahmandam M. Making Weak Antigens Strong: Modifying Protein Antigens by Denaturation. Cold Spring Harb Protoc. 2018 05 01; 2018(5). PMID: 29717055.
    Citations:    Fields:    
  27. Greenfield EA. Screening for Good Batches of Fetal Bovine Serum for Myeloma and Hybridoma Growth. Cold Spring Harb Protoc. 2018 03 01; 2018(3). PMID: 29496821.
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  28. Greenfield EA. Polyethylene Glycol Fusion for Hybridoma Production. Cold Spring Harb Protoc. 2018 03 01; 2018(3). PMID: 29496822.
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  29. Gallery M, Zhang J, Bradley DP, Brauer P, Cvet D, Estevam J, Danaee H, Greenfield E, Li P, Manfredi M, Loke HK, Rabino C, Stringer B, Williamson M, Wyant T, Yang J, Zhu Q, Abu-Yousif A, Veiby OP. A monomethyl auristatin E-conjugated antibody to guanylyl cyclase C is cytotoxic to target-expressing cells in vitro and in vivo. PLoS One. 2018; 13(1):e0191046. PMID: 29370189.
    Citations:    Fields:    Translation:HumansAnimalsCells
  30. Greenfield EA. Sampling and Preparation of Mouse and Rat Serum. Cold Spring Harb Protoc. 2017 Nov 01; 2017(11):pdb.prot100271. PMID: 29093207.
    Citations:    Fields:    Translation:Animals
  31. Greenfield EA. Harvesting Tissue from Immunized Mice, Rats, and Hamsters. Cold Spring Harb Protoc. 2017 Nov 01; 2017(11):pdb.prot100362. PMID: 29093208.
    Citations:    Fields:    Translation:AnimalsCells
  32. Mahoney KM, Sun H, Liao X, Hua P, Callea M, Greenfield EA, Hodi FS, Sharpe AH, Signoretti S, Rodig SJ, Freeman GJ. PD-L1 Antibodies to Its Cytoplasmic Domain Most Clearly Delineate Cell Membranes in Immunohistochemical Staining of Tumor Cells. Cancer Immunol Res. 2015 Dec; 3(12):1308-15. PMID: 26546452.
    Citations: 32     Fields:    Translation:HumansCells
  33. Greenfield E, Griner E. Registered report: Widespread potential for growth factor-driven resistance to anticancer kinase inhibitors. Elife. 2014 Dec 10; 3. PMID: 25490934.
    Citations:    Fields:    Translation:Humans
  34. Jones RT, Abedalthagafi MS, Brahmandam M, Greenfield EA, Hoang MP, Louis DN, Hornick JL, Santagata S. Cross-reactivity of the BRAF VE1 antibody with epitopes in axonemal dyneins leads to staining of cilia. Mod Pathol. 2015 Apr; 28(4):596-606. PMID: 25412847.
    Citations: 14     Fields:    Translation:HumansCells
  35. Rangachari M, Zhu C, Sakuishi K, Xiao S, Karman J, Chen A, Angin M, Wakeham A, Greenfield EA, Sobel RA, Okada H, McKinnon PJ, Mak TW, Addo MM, Anderson AC, Kuchroo VK. Bat3 promotes T cell responses and autoimmunity by repressing Tim-3–mediated cell death and exhaustion. Nat Med. 2012 Sep; 18(9):1394-400. PMID: 22863785.
    Citations: 64     Fields:    Translation:HumansAnimalsCells
  36. Lu X, Rios HF, Jiang B, Xing L, Kadlcek R, Greenfield EM, Luo G, Feng JQ. A new osteopetrosis mutant mouse strain (ntl) with odontoma-like proliferations and lack of tooth roots. Eur J Oral Sci. 2009 Dec; 117(6):625-35. PMID: 20121924.
    Citations: 8     Fields:    Translation:AnimalsCells
  37. Hastings WD, Anderson DE, Kassam N, Koguchi K, Greenfield EA, Kent SC, Zheng XX, Strom TB, Hafler DA, Kuchroo VK. TIM-3 is expressed on activated human CD4+ T cells and regulates Th1 and Th17 cytokines. Eur J Immunol. 2009 Sep; 39(9):2492-501. PMID: 19676072.
    Citations: 92     Fields:    Translation:HumansCells
  38. Beidelschies MA, Huang H, McMullen MR, Smith MV, Islam AS, Goldberg VM, Chen X, Nagy LE, Greenfield EM. Stimulation of macrophage TNFalpha production by orthopaedic wear particles requires activation of the ERK1/2/Egr-1 and NF-kappaB pathways but is independent of p38 and JNK. J Cell Physiol. 2008 Dec; 217(3):652-66. PMID: 18651635.
    Citations: 8     Fields:    Translation:AnimalsCells
  39. Exley MA, Hou R, Shaulov A, Tonti E, Dellabona P, Casorati G, Akbari O, Akman HO, Greenfield EA, Gumperz JE, Boyson JE, Balk SP, Wilson SB. Selective activation, expansion, and monitoring of human iNKT cells with a monoclonal antibody specific for the TCR alpha-chain CDR3 loop. Eur J Immunol. 2008 Jun; 38(6):1756-66. PMID: 18493987.
    Citations: 35     Fields:    Translation:HumansAnimalsCellsPHPublic Health
  40. Strandberg L, Ambrosi A, Espinosa A, Ottosson L, Eloranta ML, Zhou W, Elfving A, Greenfield E, Kuchroo VK, Wahren-Herlenius M. Interferon-alpha induces up-regulation and nuclear translocation of the Ro52 autoantigen as detected by a panel of novel Ro52-specific monoclonal antibodies. J Clin Immunol. 2008 May; 28(3):220-31. PMID: 18071879.
    Citations: 31     Fields:    Translation:HumansAnimalsCells
  41. Rodriguez-Manzanet R, Meyers JH, Balasubramanian S, Slavik J, Kassam N, Dardalhon V, Greenfield EA, Anderson AC, Sobel RA, Hafler DA, Strom TB, Kuchroo VK. TIM-4 expressed on APCs induces T cell expansion and survival. J Immunol. 2008 Apr 01; 180(7):4706-13. PMID: 18354194.
    Citations: 44     Fields:    Translation:AnimalsCells
  42. Cai G, Anumanthan A, Brown JA, Greenfield EA, Zhu B, Freeman GJ. CD160 inhibits activation of human CD4+ T cells through interaction with herpesvirus entry mediator. Nat Immunol. 2008 Feb; 9(2):176-85. PMID: 18193050.
    Citations: 96     Fields:    Translation:HumansCells
  43. Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Bone. 2007 May; 40(5):1276-83. PMID: 17236833.
    Citations: 12     Fields:    Translation:AnimalsCellsPHPublic Health
  44. Fan X, Rahnert JA, Murphy TC, Nanes MS, Greenfield EM, Rubin J. Response to mechanical strain in an immortalized pre-osteoblast cell is dependent on ERK1/2. J Cell Physiol. 2006 May; 207(2):454-60. PMID: 16419041.
    Citations: 20     Fields:    Translation:AnimalsCells
  45. Greenfield EA, Reddy J, Lees A, Dyer CA, Koul O, Nguyen K, Bell S, Kassam N, Hinojoza J, Eaton MJ, Lees MB, Kuchroo VK, Sobel RA. Monoclonal antibodies to distinct regions of human myelin proteolipid protein simultaneously recognize central nervous system myelin and neurons of many vertebrate species. J Neurosci Res. 2006 Feb 15; 83(3):415-31. PMID: 16416423.
    Citations: 8     Fields:    Translation:HumansAnimalsCells
  46. Bi Y, Nielsen KL, Kilts TM, Yoon A, A Karsdal M, Wimer HF, Greenfield EM, Heegaard AM, Young MF. Biglycan deficiency increases osteoclast differentiation and activity due to defective osteoblasts. Bone. 2006 Jun; 38(6):778-86. PMID: 16364709.
    Citations: 17     Fields:    Translation:AnimalsCells
  47. Dai JC, He P, Chen X, Greenfield EM. TNFalpha and PTH utilize distinct mechanisms to induce IL-6 and RANKL expression with markedly different kinetics. Bone. 2006 Apr; 38(4):509-20. PMID: 16316790.
    Citations: 17     Fields:    Translation:AnimalsCells
  48. Rubin J, Murphy TC, Rahnert J, Song H, Nanes MS, Greenfield EM, Jo H, Fan X. Mechanical inhibition of RANKL expression is regulated by H-Ras-GTPase. J Biol Chem. 2006 Jan 20; 281(3):1412-8. PMID: 16306046.
    Citations: 10     Fields:    Translation:AnimalsCells
  49. Dardalhon V, Schubart AS, Reddy J, Meyers JH, Monney L, Sabatos CA, Ahuja R, Nguyen K, Freeman GJ, Greenfield EA, Sobel RA, Kuchroo VK. CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. J Immunol. 2005 Aug 01; 175(3):1558-65. PMID: 16034094.
    Citations: 38     Fields:    Translation:AnimalsCells
  50. Chen X, Dai JC, Orellana SA, Greenfield EM. Endogenous protein kinase inhibitor gamma terminates immediate-early gene expression induced by cAMP-dependent protein kinase (PKA) signaling: termination depends on PKA inactivation rather than PKA export from the nucleus. J Biol Chem. 2005 Jan 28; 280(4):2700-7. PMID: 15557275.
    Citations: 9     Fields:    Translation:AnimalsCells
  51. Rodig N, Ryan T, Allen JA, Pang H, Grabie N, Chernova T, Greenfield EA, Liang SC, Sharpe AH, Lichtman AH, Freeman GJ. Endothelial expression of PD-L1 and PD-L2 down-regulates CD8+ T cell activation and cytolysis. Eur J Immunol. 2003 Nov; 33(11):3117-26. PMID: 14579280.
    Citations: 128     Fields:    Translation:HumansAnimalsCells
  52. Schmollinger JC, Vonderheide RH, Hoar KM, Maecker B, Schultze JL, Hodi FS, Soiffer RJ, Jung K, Kuroda MJ, Letvin NL, Greenfield EA, Mihm M, Kutok JL, Dranoff G. Melanoma inhibitor of apoptosis protein (ML-IAP) is a target for immune-mediated tumor destruction. Proc Natl Acad Sci U S A. 2003 Mar 18; 100(6):3398-403. PMID: 12626761.
    Citations: 26     Fields:    Translation:HumansAnimalsCells
  53. Brown JA, Dorfman DM, Ma FR, Sullivan EL, Munoz O, Wood CR, Greenfield EA, Freeman GJ. Blockade of programmed death-1 ligands on dendritic cells enhances T cell activation and cytokine production. J Immunol. 2003 Feb 01; 170(3):1257-66. PMID: 12538684.
    Citations: 249     Fields:    Translation:HumansAnimalsCells
  54. Meyers JH, Ryu A, Monney L, Nguyen K, Greenfield EA, Freeman GJ, Kuchroo VK. Cutting edge: CD94/NKG2 is expressed on Th1 but not Th2 cells and costimulates Th1 effector functions. J Immunol. 2002 Nov 15; 169(10):5382-6. PMID: 12421909.
    Citations: 12     Fields:    Translation:HumansAnimalsCells
  55. Howie D, Okamoto S, Rietdijk S, Clarke K, Wang N, Gullo C, Bruggeman JP, Manning S, Coyle AJ, Greenfield E, Kuchroo V, Terhorst C. The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production. Blood. 2002 Oct 15; 100(8):2899-907. PMID: 12351401.
    Citations: 21     Fields:    Translation:AnimalsCells
  56. Ragab AA, Nalepka JL, Bi Y, Greenfield EM. Cytokines synergistically induce osteoclast differentiation: support by immortalized or normal calvarial cells. Am J Physiol Cell Physiol. 2002 Sep; 283(3):C679-87. PMID: 12176725.
    Citations: 23     Fields:    Translation:AnimalsCells
  57. Akbari O, Freeman GJ, Meyer EH, Greenfield EA, Chang TT, Sharpe AH, Berry G, DeKruyff RH, Umetsu DT. Antigen-specific regulatory T cells develop via the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity. Nat Med. 2002 Sep; 8(9):1024-32. PMID: 12145647.
    Citations: 194     Fields:    Translation:AnimalsCells
  58. Eppihimer MJ, Gunn J, Freeman GJ, Greenfield EA, Chernova T, Erickson J, Leonard JP. Expression and regulation of the PD-L1 immunoinhibitory molecule on microvascular endothelial cells. Microcirculation. 2002 Apr; 9(2):133-45. PMID: 11932780.
    Citations: 84     Fields:    Translation:AnimalsCells
  59. Monney L, Sabatos CA, Gaglia JL, Ryu A, Waldner H, Chernova T, Manning S, Greenfield EA, Coyle AJ, Sobel RA, Freeman GJ, Kuchroo VK. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature. 2002 Jan 31; 415(6871):536-41. PMID: 11823861.
    Citations: 313     Fields:    Translation:HumansAnimalsCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.