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Daniel Irimia, PH.D., M.D.

TitleAssistant Professor of Surgery
InstitutionMassachusetts General Hospital
DepartmentSurgery
AddressMassachusetts General Hospital
Surgery/BioMEMS Resource Center, Rm #1404
114 16th Street
Charlestown MA 02129
Phone617/724-6543
Fax617/724-2999
vCardDownload vCard (login for email)

 Overview 
 overview
I am a bioengineer trained as a physician and I am passionate about understanding the clinical consequences of neutrophil activities during disease. My research is focused on designing sophisticated tools to measure relevant neutrophil behaviors, with the highest precision, directly from patients’ blood.

Neutrophils are the largest population of white blood cells in blood (more than 25 billion total count). During infections neutrophils move from blood to tissues, where they arrive in large numbers and destroy microbes. Consequently, the absolute neutrophil count (ANC) is the most common blood tests ordered by physicians to evaluate patients resources against infections. The implicit assumption behind ANC is that the size of the neutrophil Army is all that matters. It has even been postulated that the neutrophil-soldiers are always fast, disciplined, and effective, a status quo is reinforced by the fact that genetic diseases affecting neutrophils are extremely rare (less than 20 cases/year in the US).

The paradigm of neutrophils protective role is being challenged by emerging evidence, which suggests that neutrophil behavior changes and even becomes detrimental during diseases. The neutrophil army can fall into disarray and/or take actions that contribute to pathology, even when its size does not change. To measure the neutrophil changes during diseases, however, new tools are required. Thus, for the past several years we have been working on novel tools to make increasingly precise measurements of neutrophil activity, and living an exciting time of fascinating discoveries.

Early on, we found that neutrophils from healthy individuals can be very smart about the direction in which they move. For example, more than 90% of the human neutrophils are able to pick up the shorter route towards a chemical stimulus. We also found that neutrophils display signature-responses for a range of common chemoattractants, which are now distinguishable using microfluidic devices.

Most recently, we found that the ability of neutrophils to orient is lost in patients with severe burn injuries. We designed the first devices to measure the characteristics of neutrophil motility across 18 independent parameters and uncovered specific motility patterns for sepsis in burn patients. We found that neutrophils can move spontaneously though micron-sized channels, in the absence of directional signals, and this behavior never observed for healthy neutrophils, can predict, up to two days in advance, the occurrence of sepsis in patients with major burns. Although a direct link between the defective neutrophil motility and sepsis remains to be definitively demonstrated, we found that correcting neutrophil motility in an animal model of burn injury, can protect from complications. This observation opens the possiblity that neutrophils could be more important in monitoring and preventing sepsis than currently appreciated. New tools and better understanding of neutrophil biology will be required to answer these questions.

To accelerate the engineering of better tools for measuring cell motility and the application of these to important clinical problems, we have organized the first ever Dicty World Race. We asked scientists worldwide to apply their knowledge to make Dictyostelium and neutrophil-like cells that move faster and smarter. We then measured these cells run through challenging mazes, towards typical chemoattractants. Interesting learning are still emerging from what worked and what did not during the Race. Such knowledge could lead to better drugs to enhance white blood cell activity against microbes or slow down the movement of cancer cells during metastasis. To learn more, or just to have fun, please join us for the next Dicty World Race at https://experiment.com/projects/dicty-world-race-finding-the-fastest-and-smartest-dicty-cells.


 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Hamza B, Irimia D. Whole blood human neutrophil trafficking in a microfluidic model of infection and inflammation. Lab Chip. 2015 Jun 1; 15(12):2625-33.
    View in: PubMed
  2. Bruneau S, Wedel J, Fakhouri F, Nakayama H, Boneschansker L, Irimia D, Daly KP, Briscoe DM. Translational implications of endothelial cell dysfunction in association with chronic allograft rejection. Pediatr Nephrol. 2015 Apr 24.
    View in: PubMed
  3. Hsu CH, Chen C, Irimia D, Toner M. Fast sorting of CD4+ T cells from whole blood using glass microbubbles. Technology (Singap World Sci). 2015 Mar; 3(1):38-44.
    View in: PubMed
  4. Heo YS, Nagrath S, Moore AL, Zeinali M, Irimia D, Stott SL, Toth TL, Toner M. "Universal" vitrification of cells by ultra-fast cooling. Technology (Singap World Sci). 2015 Mar; 3(1):64-71.
    View in: PubMed
  5. Aranyosi AJ, Wong EA, Irimia D. A neutrophil treadmill to decouple spatial and temporal signals during chemotaxis. Lab Chip. 2014 Dec 16; 15(2):549-56.
    View in: PubMed
  6. Jones CN, Moore M, Dimisko L, Alexander A, Ibrahim A, Hassell BA, Warren HS, Tompkins RG, Fagan SP, Irimia D. Spontaneous Neutrophil Migration Patterns during Sepsis after Major Burns. PLoS One. 2014; 9(12):e114509.
    View in: PubMed
  7. Hori Y, Takeda S, Cho H, Wegmann S, Shoup TM, Takahashi K, Irimia D, Elmaleh DR, Hyman BT, Hudry E. A Food and Drug Administration-approved Asthma Therapeutic Agent Impacts Amyloid ß in the Brain in a Transgenic Model of Alzheimer Disease. J Biol Chem. 2015 Jan 23; 290(4):1966-78.
    View in: PubMed
  8. Nery FC, da Hora CC, Yaqub U, Zhang X, McCarthy DM, Bhide PG, Irimia D, Breakefield XO. New methods for investigation of neuronal migration in embryonic brain explants. J Neurosci Methods. 2015 Jan 15; 239:80-4.
    View in: PubMed
  9. Boneschansker L, Yan J, Wong E, Briscoe DM, Irimia D. Microfluidic platform for the quantitative analysis of leukocyte migration signatures. Nat Commun. 2014; 5:4787.
    View in: PubMed
  10. Yan J, Irimia D. Stochastic variations of migration speed between cells in clonal populations. Technology (Singap World Sci). 2014 Sep; 2(3):185-188.
    View in: PubMed
  11. Wong IY, Javaid S, Wong EA, Perk S, Haber DA, Toner M, Irimia D. Collective and individual migration following the epithelial-mesenchymal transition. Nat Mater. 2014 Nov; 13(11):1063-71.
    View in: PubMed
  12. Jones CN, Hoang AN, Dimisko L, Hamza B, Martel J, Irimia D. Microfluidic platform for measuring neutrophil chemotaxis from unprocessed whole blood. J Vis Exp. 2014; (88).
    View in: PubMed
  13. Nery FC, da Hora CC, Atai NA, Kim EY, Hettich J, Mempel TR, Breakefield XO, Irimia D. Microfluidic platform to evaluate migration of cells from patients with DYT1 dystonia. J Neurosci Methods. 2014 Jul 30; 232:181-8.
    View in: PubMed
  14. Cho H, Hamza B, Wong EA, Irimia D. On-demand, competing gradient arrays for neutrophil chemotaxis. Lab Chip. 2014 Mar 7; 14(5):972-8.
    View in: PubMed
  15. Sung Y, Lue N, Hamza B, Martel J, Irimia D, Dasari RR, Choi W, Yaqoob Z, So P. Three-Dimensional Holographic Refractive-Index Measurement of Continuously Flowing Cells in a Microfluidic Channel. Phys Rev Appl. 2014 Feb 27; 1.
    View in: PubMed
  16. Robertson AL, Holmes GR, Bojarczuk AN, Burgon J, Loynes CA, Chimen M, Sawtell AK, Hamza B, Willson J, Walmsley SR, Anderson SR, Coles MC, Farrow SN, Solari R, Jones S, Prince LR, Irimia D, Rainger GE, Kadirkamanathan V, Whyte MK, Renshaw SA. A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism. Sci Transl Med. 2014 Feb 26; 6(225):225ra29.
    View in: PubMed
  17. Hamza B, Wong E, Patel S, Cho H, Martel J, Irimia D. Retrotaxis of human neutrophils during mechanical confinement inside microfluidic channels. Integr Biol (Camb). 2014 Feb; 6(2):175-83.
    View in: PubMed
  18. Baik SH, Cha MY, Hyun YM, Cho H, Hamza B, Kim DK, Han SH, Choi H, Kim KH, Moon M, Lee J, Kim M, Irimia D, Mook-Jung I. Migration of neutrophils targeting amyloid plaques in Alzheimer's disease mouse model. Neurobiol Aging. 2014 Jun; 35(6):1286-92.
    View in: PubMed
  19. Irimia D. Cell migration in confined environments. Methods Cell Biol. 2014; 121:141-53.
    View in: PubMed
  20. Prentice-Mott HV, Chang CH, Mahadevan L, Mitchison TJ, Irimia D, Shah JV. Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments. Proc Natl Acad Sci U S A. 2013 Dec 24; 110(52):21006-11.
    View in: PubMed
  21. Hoang AN, Jones CN, Dimisko L, Hamza B, Martel J, Kojic N, Irimia D. Measuring neutrophil speed and directionality during chemotaxis, directly from a droplet of whole blood. Technology (Singap). 2013 Oct 2; 1(1):49.
    View in: PubMed
  22. Mantel PY, Hoang AN, Goldowitz I, Potashnikova D, Hamza B, Vorobjev I, Ghiran I, Toner M, Irimia D, Ivanov AR, Barteneva N, Marti M. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe. 2013 May 15; 13(5):521-34.
    View in: PubMed
  23. Desai SP, Bhatia SN, Toner M, Irimia D. Mitochondrial localization and the persistent migration of epithelial cancer cells. Biophys J. 2013 May 7; 104(9):2077-88.
    View in: PubMed
  24. Kurihara T, Jones CN, Yu YM, Fischman AJ, Watada S, Tompkins RG, Fagan SP, Irimia D. Resolvin D2 restores neutrophil directionality and improves survival after burns. FASEB J. 2013 Jun; 27(6):2270-81.
    View in: PubMed
  25. Cho H, Hashimoto T, Wong E, Hori Y, Wood LB, Zhao L, Haigis KM, Hyman BT, Irimia D. Microfluidic chemotaxis platform for differentiating the roles of soluble and bound amyloid-ß on microglial accumulation. Sci Rep. 2013; 3:1823.
    View in: PubMed
  26. Jones CN, Dalli J, Dimisko L, Wong E, Serhan CN, Irimia D. Microfluidic chambers for monitoring leukocyte trafficking and humanized nano-proresolving medicines interactions. Proc Natl Acad Sci U S A. 2012 Dec 11; 109(50):20560-5.
    View in: PubMed
  27. Bohr S, Patel SJ, Sarin D, Irimia D, Yarmush ML, Berthiaume F. Resolvin D2 prevents secondary thrombosis and necrosis in a mouse burn wound model. Wound Repair Regen. 2013 Jan-Feb; 21(1):35-43.
    View in: PubMed
  28. El-Haibi CP, Bell GW, Zhang J, Collmann AY, Wood D, Scherber CM, Csizmadia E, Mariani O, Zhu C, Campagne A, Toner M, Bhatia SN, Irimia D, Vincent-Salomon A, Karnoub AE. Critical role for lysyl oxidase in mesenchymal stem cell-driven breast cancer malignancy. Proc Natl Acad Sci U S A. 2012 Oct 23; 109(43):17460-5.
    View in: PubMed
  29. Xu C, Poh YK, Roes I, O'Cearbhaill ED, Matthiesen ME, Mu L, Yang SY, Miranda-Nieves D, Irimia D, Karp JM. A portable chemotaxis platform for short and long term analysis. PLoS One. 2012; 7(9):e44995.
    View in: PubMed
  30. Koria P, Bhushan A, Irimia D, Yarmush ML. MICROFLUIDIC DEVICE FOR EXAMINING DIRECTIONAL SENSING IN DENDRITIC CELL CHEMOTAXIS. Nano Life. 2012 Jun 1; 2(2).
    View in: PubMed
  31. Ritch JJ, Valencia A, Alexander J, Sapp E, Gatune L, Sangrey GR, Sinha S, Scherber CM, Zeitlin S, Sadri-Vakili G, Irimia D, Difiglia M, Kegel KB. Multiple phenotypes in Huntington disease mouse neural stem cells. Mol Cell Neurosci. 2012 May; 50(1):70-81.
    View in: PubMed
  32. Oh SF, Dona M, Fredman G, Krishnamoorthy S, Irimia D, Serhan CN. Resolvin E2 formation and impact in inflammation resolution. J Immunol. 2012 May 1; 188(9):4527-34.
    View in: PubMed
  33. Scherber C, Aranyosi AJ, Kulemann B, Thayer SP, Toner M, Iliopoulos O, Irimia D. Epithelial cell guidance by self-generated EGF gradients. Integr Biol (Camb). 2012 Mar; 4(3):259-69.
    View in: PubMed
  34. Aldridge BB, Fernandez-Suarez M, Heller D, Ambravaneswaran V, Irimia D, Toner M, Fortune SM. Asymmetry and aging of mycobacterial cells lead to variable growth and antibiotic susceptibility. Science. 2012 Jan 6; 335(6064):100-4.
    View in: PubMed
  35. Watkins N, Irimia D, Toner M, Bashir R. On a chip. IEEE Pulse. 2011 Nov; 2(6):19-27.
    View in: PubMed
  36. Heo YS, Lee HJ, Hassell BA, Irimia D, Toth TL, Elmoazzen H, Toner M. Controlled loading of cryoprotectants (CPAs) to oocyte with linear and complex CPA profiles on a microfluidic platform. Lab Chip. 2011 Oct 21; 11(20):3530-7.
    View in: PubMed
  37. Yang R, Fredman G, Krishnamoorthy S, Agrawal N, Irimia D, Piomelli D, Serhan CN. Decoding functional metabolomics with docosahexaenoyl ethanolamide (DHEA) identifies novel bioactive signals. J Biol Chem. 2011 Sep 9; 286(36):31532-41.
    View in: PubMed
  38. Hoerning A, Koss K, Datta D, Boneschansker L, Jones CN, Wong IY, Irimia D, Calzadilla K, Benitez F, Hoyer PF, Harmon WE, Briscoe DM. Subsets of human CD4(+) regulatory T cells express the peripheral homing receptor CXCR3. Eur J Immunol. 2011 Aug; 41(8):2291-302.
    View in: PubMed
  39. Rosenbach AE, Koria P, Goverman J, Kotz KT, Gupta A, Yu M, Fagan SP, Irimia D, Tompkins RG. Microfluidics for T- lymphocyte cell separation and inflammation monitoring in burn patients. Clin Transl Sci. 2011 Feb; 4(1):63-8.
    View in: PubMed
  40. Konry T, Smolina I, Yarmush JM, Irimia D, Yarmush ML. Ultrasensitive detection of low-abundance surface-marker protein using isothermal rolling circle amplification in a microfluidic nanoliter platform. Small. 2011 Feb 7; 7(3):395-400.
    View in: PubMed
  41. Smolen GA, Zhang J, Zubrowski MJ, Edelman EJ, Luo B, Yu M, Ng LW, Scherber CM, Schott BJ, Ramaswamy S, Irimia D, Root DE, Haber DA. A genome-wide RNAi screen identifies multiple RSK-dependent regulators of cell migration. Genes Dev. 2010 Dec 1; 24(23):2654-65.
    View in: PubMed
  42. Kane BJ, Younan G, Helm D, Dastouri P, Prentice-Mott H, Irimia D, Chan RK, Toner M, Orgill DP. Controlled induction of distributed microdeformation in wounded tissue via a microchamber array dressing. J Biomed Mater Res A. 2010 Nov; 95(2):333-40.
    View in: PubMed
  43. Prentice-Mott H, Toner M, Irimia D. Microfluidic proportional flow controller. J Micromech Microeng. 2010 Oct 15; 20(11):1-8.
    View in: PubMed
  44. Stott SL, Hsu CH, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, Floyd FP, Gilman AJ, Lord JB, Winokur D, Springer S, Irimia D, Nagrath S, Sequist LV, Lee RJ, Isselbacher KJ, Maheswaran S, Haber DA, Toner M. Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proc Natl Acad Sci U S A. 2010 Oct 26; 107(43):18392-7.
    View in: PubMed
  45. Kotz KT, Xiao W, Miller-Graziano C, Qian WJ, Russom A, Warner EA, Moldawer LL, De A, Bankey PE, Petritis BO, Camp DG, Rosenbach AE, Goverman J, Fagan SP, Brownstein BH, Irimia D, Xu W, Wilhelmy J, Mindrinos MN, Smith RD, Davis RW, Tompkins RG, Toner M. Clinical microfluidics for neutrophil genomics and proteomics. Nat Med. 2010 Sep; 16(9):1042-7.
    View in: PubMed
  46. Irimia D. Microfluidic technologies for temporal perturbations of chemotaxis. Annu Rev Biomed Eng. 2010 Aug 15; 12:259-84.
    View in: PubMed
  47. Ambravaneswaran V, Wong IY, Aranyosi AJ, Toner M, Irimia D. Directional decisions during neutrophil chemotaxis inside bifurcating channels. Integr Biol (Camb). 2010 Nov; 2(11-12):639-47.
    View in: PubMed
  48. Butler KL, Ambravaneswaran V, Agrawal N, Bilodeau M, Toner M, Tompkins RG, Fagan S, Irimia D. Burn injury reduces neutrophil directional migration speed in microfluidic devices. PLoS One. 2010; 5(7):e11921.
    View in: PubMed
  49. Wolfer A, Wittner BS, Irimia D, Flavin RJ, Lupien M, Gunawardane RN, Meyer CA, Lightcap ES, Tamayo P, Mesirov JP, Liu XS, Shioda T, Toner M, Loda M, Brown M, Brugge JS, Ramaswamy S. MYC regulation of a "poor-prognosis" metastatic cancer cell state. Proc Natl Acad Sci U S A. 2010 Feb 23; 107(8):3698-703.
    View in: PubMed
  50. Chen C, Skog J, Hsu CH, Lessard RT, Balaj L, Wurdinger T, Carter BS, Breakefield XO, Toner M, Irimia D. Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip. 2010 Feb 21; 10(4):505-11.
    View in: PubMed
  51. Irimia D. Cutting edge: electronic counting of white blood cells. Lab Chip. 2009 Oct 21; 9(20):2875-6.
    View in: PubMed
  52. Stoothoff W, Jones PB, Spires-Jones TL, Joyner D, Chhabra E, Bercury K, Fan Z, Xie H, Bacskai B, Edd J, Irimia D, Hyman BT. Differential effect of three-repeat and four-repeat tau on mitochondrial axonal transport. J Neurochem. 2009 Oct; 111(2):417-27.
    View in: PubMed
  53. Irimia D, Toner M. Spontaneous migration of cancer cells under conditions of mechanical confinement. Integr Biol (Camb). 2009 Sep; 1(8-9):506-12.
    View in: PubMed
  54. Russom A, Irimia D, Toner M. Chemical gradient-mediated melting curve analysis for genotyping of SNPs. Electrophoresis. 2009 Jul; 30(14):2536-43.
    View in: PubMed
  55. Irimia D, Balázsi G, Agrawal N, Toner M. Adaptive-control model for neutrophil orientation in the direction of chemical gradients. Biophys J. 2009 May 20; 96(10):3897-916.
    View in: PubMed
  56. Edd JF, Humphry KJ, Irimia D, Weitz DA, Toner M. Nucleation and solidification in static arrays of monodisperse drops. Lab Chip. 2009 Jul 7; 9(13):1859-65.
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  57. Kasuga K, Yang R, Porter TF, Agrawal N, Petasis NA, Irimia D, Toner M, Serhan CN. Rapid appearance of resolvin precursors in inflammatory exudates: novel mechanisms in resolution. J Immunol. 2008 Dec 15; 181(12):8677-87.
    View in: PubMed
  58. Irimia D, Mindrinos M, Russom A, Xiao W, Wilhelmy J, Wang S, Heath JD, Kurn N, Tompkins RG, Davis RW, Toner M. Genome-wide transcriptome analysis of 150 cell samples. Integr Biol (Camb). 2009 Jan; 1(1):99-107.
    View in: PubMed
  59. Agrawal N, Toner M, Irimia D. Neutrophil migration assay from a drop of blood. Lab Chip. 2008 Dec; 8(12):2054-61.
    View in: PubMed
  60. Hsu CH, Di Carlo D, Chen C, Irimia D, Toner M. Microvortex for focusing, guiding and sorting of particles. Lab Chip. 2008 Dec; 8(12):2128-34.
    View in: PubMed
  61. Nery FC, Zeng J, Niland BP, Hewett J, Farley J, Irimia D, Li Y, Wiche G, Sonnenberg A, Breakefield XO. TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton. J Cell Sci. 2008 Oct 15; 121(Pt 20):3476-86.
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  62. Prabhakarpandian B, Pant K, Scott RC, Pattillo CB, Patillo CB, Irimia D, Kiani MF, Sundaram S. Synthetic microvascular networks for quantitative analysis of particle adhesion. Biomed Microdevices. 2008 Aug; 10(4):585-95.
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  63. Maheswaran S, Sequist LV, Nagrath S, Ulkus L, Brannigan B, Collura CV, Inserra E, Diederichs S, Iafrate AJ, Bell DW, Digumarthy S, Muzikansky A, Irimia D, Settleman J, Tompkins RG, Lynch TJ, Toner M, Haber DA. Detection of mutations in EGFR in circulating lung-cancer cells. N Engl J Med. 2008 Jul 24; 359(4):366-77.
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  64. Edd JF, Di Carlo D, Humphry KJ, Köster S, Irimia D, Weitz DA, Toner M. Controlled encapsulation of single-cells into monodisperse picolitre drops. Lab Chip. 2008 Aug; 8(8):1262-4.
    View in: PubMed
  65. Park K, Jang J, Irimia D, Sturgis J, Lee J, Robinson JP, Toner M, Bashir R. 'Living cantilever arrays' for characterization of mass of single live cells in fluids. Lab Chip. 2008 Jul; 8(7):1034-41.
    View in: PubMed
  66. Kelly RT, Tang K, Irimia D, Toner M, Smith RD. Elastomeric microchip electrospray emitter for stable cone-jet mode operation in the nanoflow regime. Anal Chem. 2008 May 15; 80(10):3824-31.
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  67. Parekkadan B, Berdichevsky Y, Irimia D, Leeder A, Yarmush G, Toner M, Levine JB, Yarmush ML. Cell-cell interaction modulates neuroectodermal specification of embryonic stem cells. Neurosci Lett. 2008 Jun 20; 438(2):190-5.
    View in: PubMed
  68. Russom A, Sethu P, Irimia D, Mindrinos MN, Calvano SE, Garcia I, Finnerty C, Tannahill C, Abouhamze A, Wilhelmy J, López MC, Baker HV, Herndon DN, Lowry SF, Maier RV, Davis RW, Moldawer LL, Tompkins RG, Toner M. Microfluidic leukocyte isolation for gene expression analysis in critically ill hospitalized patients. Clin Chem. 2008 May; 54(5):891-900.
    View in: PubMed
  69. Di Carlo D, Edd JF, Irimia D, Tompkins RG, Toner M. Equilibrium separation and filtration of particles using differential inertial focusing. Anal Chem. 2008 Mar 15; 80(6):2204-11.
    View in: PubMed
  70. Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M. Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 2007 Dec 20; 450(7173):1235-9.
    View in: PubMed
  71. Di Carlo D, Irimia D, Tompkins RG, Toner M. Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci U S A. 2007 Nov 27; 104(48):18892-7.
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  72. Vu HN, Li Y, Casali M, Irimia D, Megeed Z, Yarmush ML. A microfluidic bioreactor for increased active retrovirus output. Lab Chip. 2008 Jan; 8(1):75-80.
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  73. Irimia D, Charras G, Agrawal N, Mitchison T, Toner M. Polar stimulation and constrained cell migration in microfluidic channels. Lab Chip. 2007 Dec; 7(12):1783-90.
    View in: PubMed
  74. Cheng X, Irimia D, Dixon M, Ziperstein JC, Demirci U, Zamir L, Tompkins RG, Toner M, Rodriguez WR. A microchip approach for practical label-free CD4+ T-cell counting of HIV-infected subjects in resource-poor settings. J Acquir Immune Defic Syndr. 2007 Jul 1; 45(3):257-61.
    View in: PubMed
  75. Cheng X, Liu YS, Irimia D, Demirci U, Yang L, Zamir L, Rodríguez WR, Toner M, Bashir R. Cell detection and counting through cell lysate impedance spectroscopy in microfluidic devices. Lab Chip. 2007 Jun; 7(6):746-55.
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  76. Daniel Irimia. Pneumatic Valves in Encyclopedia of Micro- and Nanofluidics edited by Li, Dongqing. 2006.
  77. Daniel Irimia. Techniques of Manipulating Cells in Encyclopedia of Micro- and Nanofluidics edited by Li, Dongqing. 2006.
  78. Cheng X, Irimia D, Dixon M, Sekine K, Demirci U, Zamir L, Tompkins RG, Rodriguez W, Toner M. A microfluidic device for practical label-free CD4(+) T cell counting of HIV-infected subjects. Lab Chip. 2007 Feb; 7(2):170-8.
    View in: PubMed
  79. King KR, Wang S, Irimia D, Jayaraman A, Toner M, Yarmush ML. A high-throughput microfluidic real-time gene expression living cell array. Lab Chip. 2007 Jan; 7(1):77-85.
    View in: PubMed
  80. Irimia D, Geba DA, Toner M. Universal microfluidic gradient generator. Anal Chem. 2006 May 15; 78(10):3472-7.
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  81. Vianello F, Papeta N, Chen T, Kraft P, White N, Hart WK, Kircher MF, Swart E, Rhee S, Palù G, Irimia D, Toner M, Weissleder R, Poznansky MC. Murine B16 melanomas expressing high levels of the chemokine stromal-derived factor-1/CXCL12 induce tumor-specific T cell chemorepulsion and escape from immune control. J Immunol. 2006 Mar 1; 176(5):2902-14.
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  82. Irimia D, Toner M. Cell handling using microstructured membranes. Lab Chip. 2006 Mar; 6(3):345-52.
    View in: PubMed
  83. Daniel Irimia. Capillary Force Valves in Encyclopedia of Micro- and Nanofluidics edited by Li, Dongqing. 2006.
  84. Aksan A, Irimia D, He X, Toner M. Desiccation kinetics of biopreservation solutions in microchannels. J Appl Phys. 2006; 99(6):2181280.
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  85. Irimia D, Liu SY, Tharp WG, Samadani A, Toner M, Poznansky MC. Microfluidic system for measuring neutrophil migratory responses to fast switches of chemical gradients. Lab Chip. 2006 Feb; 6(2):191-8.
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  86. Tharp WG, Yadav R, Irimia D, Upadhyaya A, Samadani A, Hurtado O, Liu SY, Munisamy S, Brainard DM, Mahon MJ, Nourshargh S, van Oudenaarden A, Toner MG, Poznansky MC. Neutrophil chemorepulsion in defined interleukin-8 gradients in vitro and in vivo. J Leukoc Biol. 2006 Mar; 79(3):539-54.
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  87. Toner M, Irimia D. Blood-on-a-chip. Annu Rev Biomed Eng. 2005; 7:77-103.
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  88. Irimia D, Tompkins RG, Toner M. Single-cell chemical lysis in picoliter-scale closed volumes using a microfabricated device. Anal Chem. 2004 Oct 15; 76(20):6137-43.
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  89. Irimia D, Karlsson JO. Kinetics of intracellular ice formation in one-dimensional arrays of interacting biological cells. Biophys J. 2005 Jan; 88(1):647-60.
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  90. Stott SL, Irimia D, Karlsson JO. Parametric analysis of intercellular ice propagation during cryosurgery, simulated using monte carlo techniques. Technol Cancer Res Treat. 2004 Apr; 3(2):113-23.
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  91. Irimia, D., Karlsson, J.O.M. Development of a Cell Patterning Technique Using Poly(Ethylene Glycol) Disilane. Journal of Biomedical Microdevices. 2003; 5(3):185-194.
  92. Irimia D, Karlsson JO. Kinetics and mechanism of intercellular ice propagation in a micropatterned tissue construct. Biophys J. 2002 Apr; 82(4):1858-68.
    View in: PubMed
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