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One or more keywords matched the following properties of Asara, John
keywords Mass Spectrometry
overview Research activities in the Asara lab focusses on the development of merging tri-omics (proteomics, metabolomics and lipidomics) data. We apply these methods for a personalized medicine approach by analyzing cancer cells and tumor samples in order help make appropriate therapeutic decisions with "smart" drugs. This is accomplished by understanding how cancer cells are signaling for growth and proliferation and how signaling talks to metabolic processes in order to develop mass spec quantitative assays. We are developing hypothesis directed proteomics and gloabl metabolomics and lipidomics strategies to understand the fundamental drivers of disease in cancers such as multiple myeloma, lung cancer and prostate cancer by performing protein-protein interaction studies, immunoprecipitation (IP)-tandem mass spectrometry (MS/MS), quantitative phosphoproteomics, metabolic and lipidomic profiling as well as metabolic flux analyses. Taken together, this information is vital in selecting the appropriate use of newly developed drugs. In addition to our internal research, we are involved in many collaborative projects that are coordinated through our state-of-the-art high resolution mass spectrometry technologies and services through our mass spectrometry facility.
One or more keywords matched the following items that are connected to Asara, John
Item TypeName
Academic Article A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain.
Academic Article Analyses of soft tissue from Tyrannosaurus rex suggest the presence of protein.
Academic Article Data pre-processing in liquid chromatography-mass spectrometry-based proteomics.
Academic Article Interpreting sequences from mastodon and T. rex.
Academic Article Enhanced detection of phosphopeptides in matrix-assisted laser desorption/ionization mass spectrometry using ammonium salts.
Academic Article In-Gel Stable-Isotope Labeling (ISIL): a strategy for mass spectrometry-based relative quantification.
Academic Article Determining in vivo phosphorylation sites using mass spectrometry.
Academic Article Proteomic and metabolomic profiling reveals time-dependent changes in hippocampal metabolism upon paroxetine treatment and biomarker candidates.
Academic Article Protein sequences from mastodon and Tyrannosaurus rex revealed by mass spectrometry.
Academic Article Proteomic screening method for phosphopeptide motif binding proteins using peptide libraries.
Academic Article Metabolomic profiling from formalin-fixed, paraffin-embedded tumor tissue using targeted LC/MS/MS: application in sarcoma.
Academic Article Casein kinase 1 proteomics reveal prohibitin 2 function in molecular clock.
Academic Article HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing.
Academic Article Use of a label-free quantitative platform based on MS/MS average TIC to calculate dynamics of protein complexes in insulin signaling.
Academic Article Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism.
Academic Article Using tandem mass spectrometry in targeted mode to identify activators of class IA PI3K in cancer.
Academic Article The Crohn's disease protein, NOD2, requires RIP2 in order to induce ubiquitinylation of a novel site on NEMO.
Academic Article Enhanced detection of oligonucleotides in UV MALDI MS using the tetraamine spermine as a matrix additive.
Academic Article Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors.
Academic Article A positive/negative ion-switching, targeted mass spectrometry-based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue.
Academic Article MEK inhibition leads to PI3K/AKT activation by relieving a negative feedback on ERBB receptors.
Academic Article In-gel stable isotope labeling for relative quantification using mass spectrometry.
Academic Article C3b deposition on human erythrocytes induces the formation of a membrane skeleton-linked protein complex.
Academic Article Serum proteomics and biomarkers in hepatocellular carcinoma and chronic liver disease.
Academic Article Biomolecular characterization and protein sequences of the Campanian hadrosaur B. canadensis.
Academic Article Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1.
Academic Article Detection of a rare BCR-ABL tyrosine kinase fusion protein in H929 multiple myeloma cells using immunoprecipitation (IP)-tandem mass spectrometry (MS/MS).
Academic Article Annexin A2 is a molecular target for TM601, a peptide with tumor-targeting and anti-angiogenic effects.
Academic Article Phosphoinositide 3-kinase pathway activation in phosphate and tensin homolog (PTEN)-deficient prostate cancer cells is independent of receptor tyrosine kinases and mediated by the p110beta and p110delta catalytic subunits.
Academic Article Metabolomics of human cerebrospinal fluid identifies signatures of malignant glioma.
Academic Article A label-free quantification method by MS/MS TIC compared to SILAC and spectral counting in a proteomics screen.
Academic Article Regulation and identification of Na,K-ATPase alpha1 subunit phosphorylation in rat parotid acinar cells.
Academic Article Raptor is phosphorylated by cdc2 during mitosis.
Concept Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Concept Spectrometry, Mass, Secondary Ion
Concept Tandem Mass Spectrometry
Academic Article Tyrosine kinase BMX phosphorylates phosphotyrosine-primed motif mediating the activation of multiple receptor tyrosine kinases.
Academic Article Characterization and analysis of the composition and dynamics of the mammalian riboproteome.
Academic Article Behavioral extremes of trait anxiety in mice are characterized by distinct metabolic profiles.
Academic Article Gross cystic disease fluid protein-15/prolactin-inducible protein as a biomarker for keratoconus disease.
Academic Article Tear metabolite changes in keratoconus.
Academic Article Gain of glucose-independent growth upon metastasis of breast cancer cells to the brain.
Academic Article The ABRF Metabolomics Research Group 2013 Study: Investigation of Spiked Compound Differences in a Human Plasma Matrix.
Academic Article A Cross-Species Study of PI3K Protein-Protein Interactions Reveals the Direct Interaction of P85 and SHP2.
Academic Article Selective Mitochondrial Targeting Exerts Anxiolytic Effects In Vivo.
Academic Article Endocrine and Metabolic Pathways Linked to Keratoconus: Implications for the Role of Hormones in the Stromal Microenvironment.
Grant High Resolution Mass Spectrometry System
Academic Article An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling.
Academic Article Serial-omics of P53-/-, Brca1-/- Mouse Breast Tumor and Normal Mammary Gland.
Academic Article Ex vivo and in vivo stable isotope labelling of central carbon metabolism and related pathways with analysis by LC-MS/MS.
Academic Article Direct stimulation of NADP+ synthesis through Akt-mediated phosphorylation of NAD kinase.
Search Criteria
  • Mass Spectrometry
Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.