Associate Professor of Medicine
Beth Israel Deaconess Medical Center
Center for Life Science
BIDMC - Rm 409
3 Blackfan Circle
Boston MA 02115
|Brandeis University, Waltham, MA||BA||05/1995||Chemistry|
|Michigan State University, East Lansing, MI||Ph.D.||12/1999||Analytical Chemistry|
|Harvard University, Cambridge, MA||PostDoc||04/2002||Proteomics|
10 out of 100 Top Scientific Discoveries
Young Investigator Award
Faculty of 1000, Biology, Signal Transduction
Outstanding Poster Award
Shared Instrumentation Grant Award
Capital Equipment Award
Academic Partnership Award
Research activities in the Asara lab focusses on the development of merging tri-omics (proteomics, metabolomics and lipidomics) data. We apply these methods for a personalized medicine approach by analyzing cancer cells and tumor samples in order help make appropriate therapeutic decisions with "smart" drugs. This is accomplished by understanding how cancer cells are signaling for growth and proliferation and how signaling talks to metabolic processes in order to develop mass spec quantitative assays. We are developing hypothesis directed proteomics and gloabl metabolomics and lipidomics strategies to understand the fundamental drivers of disease in cancers such as multiple myeloma, lung cancer and prostate cancer by performing protein-protein interaction studies, immunoprecipitation (IP)-tandem mass spectrometry (MS/MS), quantitative phosphoproteomics, metabolic and lipidomic profiling as well as metabolic flux analyses. Taken together, this information is vital in selecting the appropriate use of newly developed drugs. In addition to our internal research, we are involved in many collaborative projects that are coordinated through our state-of-the-art high resolution mass spectrometry technologies and services through our mass spectrometry facility.
Available: 11/01/18, Expires: 10/31/19
The Asara lab and BIDMC mass spectrometry core perform metabolomic, lipidomic, and proteomic profiling on various samples sources including tissues, cells, bodily fluids, etc. We apply a variety of analyses including metabolic flux analysis, unlabeled profiling, phosphoproteomics, etc. in an effort to integrate these data and learn about unique pathways of diseases such as cancer or processes in various organisms. Our technologies are state of the art and all mass spectrometry and bioinformatics based. We use non-targeted high resolution and targeted MS methods with LC-MS/MS workflows.
Available: 11/01/18, Expires: 06/01/19
The project aims to use tandem mass spectrometry (LC/MS/MS) to assess the signaling pathways in tumors in order to quantitatively determine the drivers of cancers from cell receptors to downstream kinases. The student will perform immunoprecipitations (IP) of key signaling nodes in cell and tumor lysates and then based on the quantitative mass spectrometry protein-protein interaction (PPI) data, validate the findings by choosing the appropriate single agent or combinations of tyrosine kinase inhibitor (TKI) drugs in order to both shut down the affected signaling pathways and kill the cancer cells. The students should have a strong background in biochemistry and have some experience and interest in both mass spectrometry and HPLC.
The research activities and funding listed below are automatically derived from
NIH ExPORTER and other sources, which might result in incorrect or missing items.
to make corrections and additions.
(ASARA, JOHN M)
May 1, 2012 - Apr 30, 2013
High Resolution Mass Spectrometry System
Role: Principal Investigator
(KWIATKOWSKI, DAVID J.)
Apr 1, 2006 - Jul 31, 2017
Molecular Pathogenesis of the Hamartoma Syndromes
Role: Co-Principal Investigator
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