Martha Leonia Bulyk, Ph.D.
Title Professor of Medicine Institution Brigham and Women's Hospital Address Harvard Medical School Genetics/Medicine, HMS NRB 466d 77 Avenue Louis Pasteur Boston MA 02115
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Title
Professor of Pathology
Institution
Brigham and Women's Hospital
Title
Temp Course Instructor, DBMI
Institution
Harvard Medical School
Department
Biomedical Informatics

Mentoring
Available: 12/14/22, Expires: 12/01/23
More than 40 coding variants within the DNA-binding domain of the transcription factor KLF1 have been associated with a wide spectrum of red blood cell disorders and rare blood cell traits, spanning phenotypes such as clinically unremarkable blood group types and the benign hereditary persistence of fetal hemoglobin, to more severe diseases such as nonspherocytic hemolytic anemia and congenital dyserythropoietic anemia. To investigate possible differences in the gene regulatory effects of these variants that may result in differences in gene dysregulation, we created and profiled an allelic series of KLF1 coding variants. Briefly, by CRISPR/Cas9-mediated genome editing, we have created isogenic cell lines of KLF1 missense variants in the K562 human erythroleukemic cell line. We then performed protein-binding microarrays (PBMs), CUT&RUN, ATAC-seq, and RNA-seq on this allelic series, in order to determine the effects of these KLF1 variants on in vitro DNA binding activity, genomic occupancy in cells, chromatin accessibility, and gene expression. This project will focus on integrative computational analysis of these collected functional genomic datasets in order to better understand the relationship between the altered DNA binding activities of these KLF1 variants and their effects on gene regulation. This project has the potential to also involve some wetlab experiments to investigate the associated cellular phenotypes. The student working on this project will take a lead role on the computational analysis and potential wetlab experimentation, and will contribute the results to a manuscript in preparation. Students interested in this opportunity should have prior experience in analyzing genomic datasets and Illumina sequencing data.

Research
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R03HD099358
(BULYK, MARTHA L)
Sep 1, 2019 - Aug 31, 2021
Transcription factor mutationsunderlying birth defects or pediatric cancers
Role: Principal Investigator
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R01HG010501
(BULYK, MARTHA L)
May 1, 2019 - Jun 30, 2023
Impact of Coding Variation on Transcription Factor - DNA Recognition
Role: Principal Investigator
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U01CA232161
(VIDAL, MARC)
Sep 17, 2018 - Aug 31, 2023
Rewiring of regulatory networks in breast cancer by transcription factor isoforms
Role: Co-Principal Investigator
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R21HG010200
(BULYK, MARTHA L)
Aug 20, 2018 - May 31, 2021
AVATAR: highly parallel analysis of variation in transcription factors and their DNA binding sites
Role: Principal Investigator
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R01HG009723
(BULYK, MARTHA L)
Aug 1, 2017 - May 31, 2021
Transcriptional silencers inDrosophila
Role: Principal Investigator
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R21HG009268
(BULYK, MARTHA L)
Sep 28, 2016 - Jul 31, 2020
Surveying transcription factor pioneer interactions with nucleosomal DNA
Role: Principal Investigator
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R21HG007573
(BULYK, MARTHA L)
Sep 4, 2014 - Aug 31, 2017
Analysis of TALE-DNA Recognition
Role: Principal Investigator
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R01GM104062
(BULYK, MARTHA L)
Aug 10, 2013 - Mar 31, 2018
Drosophila Transcription Factor Interactome
Role: Principal Investigator
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R03AI092442
(BULYK, MARTHA L)
Dec 1, 2010 - Aug 31, 2013
Identifying novel small molecules for improved antifungal drug treatment
Role: Principal Investigator
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R01HG005287
(BULYK, MARTHA L)
Dec 1, 2009 - Jan 31, 2018
High-Throughput Analysis of Tissue/Cell-Type-Specific Cis Regulatory Modules
Role: Principal Investigator
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R21HG005149
(BULYK, MARTHA L)
Sep 1, 2009 - Dec 31, 2011
Physical and Functional Interactions of Cis Regulatory Modules
Role: Principal Investigator
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R01HG003985
(BULYK, MARTHA L)
Jul 26, 2006 - Jan 31, 2018
High-Resolution Analysis of Transcription Factors' DNA Binding Specificities
Role: Principal Investigator
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U01GM076603
(BULYK, MARTHA L)
Feb 1, 2006 - Jan 31, 2010
Integrated Genomic Analysis of a Drosophila Transcriptional Regulatory Network
Role: Principal Investigator
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U01HL081755
(BULYK, MARTHA L)
Sep 1, 2005 - May 31, 2010
Transcriptional control of Drosophila heart development
Role: Principal Investigator
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R01HG003420
(BULYK, MARTHA L)
Sep 24, 2004 - Mar 31, 2009
Yeast Transcription Factor Binding Specificities
Role: Principal Investigator
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R01HG002966
(BULYK, MARTHA L)
Sep 19, 2003 - Dec 30, 2008
Eukaryotic Transcription Factor Binding Site Locations
Role: Principal Investigator

Bibliographic
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