Harvard Catalyst Profiles

Contact, publication, and social network information about Harvard faculty and fellows.

Martha Leonia Bulyk, Ph.D.

Title
Institution
Department
Address
Phone
Fax
Other Positions
Title
Institution
Department
Title
Institution
Department

Mentoring
Available: 12/14/22, Expires: 12/01/23

More than 40 coding variants within the DNA-binding domain of the transcription factor KLF1 have been associated with a wide spectrum of red blood cell disorders and rare blood cell traits, spanning phenotypes such as clinically unremarkable blood group types and the benign hereditary persistence of fetal hemoglobin, to more severe diseases such as nonspherocytic hemolytic anemia and congenital dyserythropoietic anemia. To investigate possible differences in the gene regulatory effects of these variants that may result in differences in gene dysregulation, we created and profiled an allelic series of KLF1 coding variants. Briefly, by CRISPR/Cas9-mediated genome editing, we have created isogenic cell lines of KLF1 missense variants in the K562 human erythroleukemic cell line. We then performed protein-binding microarrays (PBMs), CUT&RUN, ATAC-seq, and RNA-seq on this allelic series, in order to determine the effects of these KLF1 variants on in vitro DNA binding activity, genomic occupancy in cells, chromatin accessibility, and gene expression. This project will focus on integrative computational analysis of these collected functional genomic datasets in order to better understand the relationship between the altered DNA binding activities of these KLF1 variants and their effects on gene regulation. This project has the potential to also involve some wetlab experiments to investigate the associated cellular phenotypes. The student working on this project will take a lead role on the computational analysis and potential wetlab experimentation, and will contribute the results to a manuscript in preparation. Students interested in this opportunity should have prior experience in analyzing genomic datasets and Illumina sequencing data.


Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R03HD099358 (BULYK, MARTHA L) Sep 1, 2019 - Aug 31, 2021
    NIH
    Transcription factor mutationsunderlying birth defects or pediatric cancers
    Role: Principal Investigator
  2. R01HG010501 (BULYK, MARTHA L) May 1, 2019 - Jun 30, 2023
    NIH
    Impact of Coding Variation on Transcription Factor - DNA Recognition
    Role: Principal Investigator
  3. U01CA232161 (VIDAL, MARC) Sep 17, 2018 - Aug 31, 2023
    NIH
    Rewiring of regulatory networks in breast cancer by transcription factor isoforms
    Role: Co-Principal Investigator
  4. R21HG010200 (BULYK, MARTHA L) Aug 20, 2018 - May 31, 2021
    NIH
    AVATAR: highly parallel analysis of variation in transcription factors and their DNA binding sites
    Role: Principal Investigator
  5. R01HG009723 (BULYK, MARTHA L) Aug 1, 2017 - May 31, 2021
    NIH
    Transcriptional silencers inDrosophila
    Role: Principal Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
Updating...
This operation might take several minutes to complete. Please do not close your browser.
Local representatives can answer questions about the Profiles website or help with editing a profile or issues with profile data. For assistance with this profile: HMS/HSDM faculty should contact contactcatalyst.harvard.edu. For faculty or fellow appointment updates and changes, please ask your appointing department to contact HMS. For fellow personal and demographic information, contact HMS Human Resources at human_resourceshms.harvard.edu. For faculty personal and demographic information, contact HMS Office for Faculty Affairs at facappthms.harvard.edu.
Bulyk's Networks
Click the
Explore
buttons for more information and interactive visualizations!
Concepts (440)
Explore
_
Co-Authors (71)
Explore
_
Similar People (60)
Explore
_
Same Department 
Explore
_
Physical Neighbors
_
Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.