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Martin Purschke, Ph.D.

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Biography
University Hospital Hamburg Eppendorf, GermanyPhD06/2004Radiation Biology
University of Oldenburg, GermanyMS06/1999Biochemistry
2010 - 2011
ASLMS Reseach Grant
2017 - 2018
STTR-UV-emitting nanoparticles as novel radiation sensitizers targeting hypoxic cells

Overview
I am an Instructor in Dermatology at the Wellman Center for Photomedicine. During my Ph.D. theses, I studied radiation induced DNA damage and got interested in the field of radiation oncology research. I started my first postdoctoral fellowship in 2005 at the Radiation Oncology Department at MGH continuing my previous research on radiation induced DNA damage. During my second post doctoral fellowship at the Dermatology Department I was involved in several translational research projects such as heat and lidocaine sensitivity on cells or skin tissue engineering. In 2010 I was promoted to an instructor in Dermatology. 3 years ago, I combined my radiation oncology background with my achieved translational research knowledge and started 2 new projects involving light and heat to treat cancer:
One is focusing on the stress response of non-heated cells which are in close proximity to heated ones. Recently, I discovered that DNA damage and loss of cell viability can be induced in human cells simply by being co-cultured with previously heat exposed cells. Subsequently, I demonstrated that this “Active Thermal Bystander Effect” (ATBE) is cell cycle dependent. The in vitro data demonstrated that cells which are actively participating in the cell cycle exhibit a significant higher ATBE, whereas non-dividing or differentiated cells do not exhibit an ATBE. In addition, I observed an increased level of ATBE in more active cell cycling cancer cells (HeLa). These in vitro data were the foundation to investigate the ATBE in vivo to selectively target cancer cells. In a subcutaneous cancer model in mice I observed an up to 30% reduction of tumor growth after induction of the ATBE compare to non-treated control mice. These highly promising in vivo data suggest that the use of remote moderate heat as an adjuvant treatment to conventional cancer treatment might result in an increased tumor control.
The second approach to improve cancer treatment is the deliver UV-C emitting nanoparticles into tumor tissue. These specific nanoparticles emit UV-C once excited with x-ray. These UV photons are more potent to damage the DNA in close proximity due to a direct and O2 independent interaction. The additional UV-C photons kill more tumor cells, in particular in hypoxic tumor regions which are typically more radiationresistant. Currently, I am testing different compounds in respect to UV-C emission efficiency and tumor deliver. The first in vitro data support the hypothesis and showed an increased cell death after UV-C emitting nanoparticle treatment combined with convetional x-ray radiation. Irradiation with 2 Gy with UV emitting nanoparticles resulted in a surviving fraction which is equivalent to a 4 Gy radiation alone.
With my current UV nanoparticles project, I started my own group in 2016 within my supervisor Dr. Andersons group and hired a postdoctoral fellow, who will support this project. I focus on light emitting nanoparticles for medical problems. I already got awarded an STTR grant to continue the UV nanoparticle project and an institutional discovery grant to study blue light emitting nanoparticles to treat wound infections.

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Garibyan L, Moradi Tuchayi S, Javorsky E, Farinelli WA, Wang Y, Purschke M, Tam J, Ni P, Lian CG, Anderson RR. Subcutaneous Fat Reduction with Injected Ice Slurry. Plast Reconstr Surg. 2020 Apr; 145(4):725e-733e. PMID: 32221206.
    Citations:    
  2. Tam J, Purschke M, Fuchs C, Wang Y, Anderson RR. Skin Microcolumns as a Source of Paracrine Signaling Factors. Adv Wound Care (New Rochelle). 2020 04 01; 9(4):174-183. PMID: 32117581.
    Citations:    
  3. Garibyan L, Moradi Tuchayi S, Wang Y, Khodorova A, Stemmer-Rachamimov A, Purschke M, Osseiran S, Evans CL, Mao J, Strichartz G, Anderson RR. Neural Selective Cryoneurolysis with Ice Slurry Injection in a Rat Model. Anesthesiology. 2020 Jan 15. PMID: 31977524.
    Citations:    
  4. Garibyan L, Moradi Tuchayi S, Javorsky E, Farinelli WA, Wang Y, Purschke M, Tam J, Ni P, Lian CG, Anderson RR. Subcutaneous Fat Reduction with Injected Ice Slurry. Plast Reconstr Surg. 2020 Jan 07. PMID: 31917745.
    Citations:    
  5. Müller M, Espinoza S, Jüstel T, Held KD, Anderson RR, Purschke M. UVC-Emitting LuPO4:Pr3+ Nanoparticles Decrease Radiation Resistance of Hypoxic Cancer Cells. Radiat Res. 2020 01; 193(1):82-87. PMID: 31738663.
    Citations:    
  6. Russe E, Purschke M, Herold M, Sakamoto FH, Wechselberger G, Russe-Wilflingseder K. Evaluation of Safety and Efficacy of Laser Hair Removal With the Long-Pulsed 755?nm Wavelength Laser: A Two-Center Study With 948 Patients. Lasers Surg Med. 2020 Jan; 52(1):77-83. PMID: 31579971.
    Citations:    
  7. Raff AB, Thomas CN, Chuang GS, Avram MM, Le MH, Anderson RR, Purschke M. Lidocaine-induced potentiation of thermal damage in skin and carcinoma cells. Lasers Surg Med. 2019 01; 51(1):88-94. PMID: 30320907.
    Citations:    Fields:    
  8. Schwaiger K, Panzenbeck P, Purschke M, Russe E, Kaplan R, Heinrich K, Mandal P, Wechselberger G. Surgical decompression of the lateral femoral cutaneous nerve (LFCN) for Meralgia paresthetica treatment: Experimental or state of the art? A single-center outcome analysis. Medicine (Baltimore). 2018 Aug; 97(33):e11914. PMID: 30113491.
    Citations:    Fields:    Translation:Humans
  9. Müller M, Wang Y, Squillante MR, Held KD, Anderson RR, Purschke M. UV scintillating particles as radiosensitizer enhance cell killing after X-ray excitation. Radiother Oncol. 2018 12; 129(3):589-594. PMID: 30539764.
    Citations:    Fields:    
  10. Squillante MR, Jüstel T, Anderson RR, Brecher C, Chartier D, Christian JF, Cicchetti N, Espinoza S, McAdams DR, Müller M, Tornifoglio B, Wang Y, Purschke M. Fabrication and characterization of UV-emitting nanoparticles as novel radiation sensitizers targeting hypoxic tumor cells. Opt Mater (Amst). 2018 Jun; 80:197-202. PMID: 30692715.
    Citations:    
  11. Russe E, Purschke M, Limpiangkanan W, Farinelli WA, Wang Y, Doukas AG, Sakamoto FH, Wechselberger G, Anderson RR. Significant skin-tightening by closure of fractional ablative laser holes. Lasers Surg Med. 2018 01; 50(1):64-69. PMID: 29058788.
    Citations:    Fields:    
  12. Jank BJ, Goverman J, Guyette JP, Charest JM, Randolph M, Gaudette GR, Gershlak JR, Purschke M, Javorsky E, Nazarian RM, Leonard DA, Cetrulo CL, Austen WG, Ott HC. Creation of a Bioengineered Skin Flap Scaffold with a Perfusable Vascular Pedicle. Tissue Eng Part A. 2017 07; 23(13-14):696-707. PMID: 28323545.
    Citations: 2     Fields:    Translation:Animals
  13. Russe E, Purschke M, Farinelli WA, Wang Y, Doukas AG, Limpiangkanan W, Sakamoto FH, Tam J, Wechselberger G, Anderson RR. Micro-fractional, directional skin tightening: A porcine model. Lasers Surg Med. 2016 Mar; 48(3):264-9. PMID: 26627306.
    Citations: 1     Fields:    Translation:Animals
  14. Tran TN, Hoang MV, Phan QA, Phung TL, Purschke M, Ferinelli WA, Sabir S, Ziegler A, Nelson S, Anderson RR. Fractional epidermal grafting in combination with laser therapy as a novel approach in treating radiation dermatitis. Semin Cutan Med Surg. 2015 Mar; 34(1):42-7. PMID: 25922957.
    Citations: 3     Fields:    
  15. Purschke M, Asrani FA, Sabir SA, Farinelli WA, Anderson RR. Novel methods for generating fractional epidermal micrografts. Br J Dermatol. 2015 Apr; 172(4):1021-8. PMID: 25307844.
    Citations:    Fields:    Translation:HumansCells
  16. Tam J, Wang Y, Farinelli WA, Jiménez-Lozano J, Franco W, Sakamoto FH, Cheung EJ, Purschke M, Doukas AG, Anderson RR. Fractional Skin Harvesting: Autologous Skin Grafting without Donor-site Morbidity. Plast Reconstr Surg Glob Open. 2013 Sep; 1(6):e47. PMID: 25289241.
    Citations: 3     
  17. Chiu CY, Gomolka B, Dierkes C, Huang NR, Schroeder M, Purschke M, Manstein D, Dangi B, Weylandt KH. Erratum to: Omega-6 docosapentaenoic acid-derived resolvins and 17-hydroxydocosahexaenoic acid modulate macrophage function and alleviate experimental colitis. Inflamm Res. 2012 Nov; 61(11):1293. PMID: 22975931.
    Citations:    
  18. Chiu CY, Gomolka B, Dierkes C, Huang NR, Schroeder M, Purschke M, Manstein D, Dangi B, Weylandt KH. Omega-6 docosapentaenoic acid-derived resolvins and 17-hydroxydocosahexaenoic acid modulate macrophage function and alleviate experimental colitis. Inflamm Res. 2012 Sep; 61(9):967-76. PMID: 22618200.
    Citations: 14     Fields:    Translation:AnimalsCells
  19. Purschke M, Anderson RR, Zurakowski D, Manstein D. Cell-cycle-dependent active thermal bystander effect (ATBE). Lasers Surg Med. 2011 Mar; 43(3):230-5. PMID: 21308697.
    Citations: 2     Fields:    Translation:HumansCells
  20. Purschke M, Rubio N, Held KD, Redmond RW. Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy. Photochem Photobiol Sci. 2010 Dec; 9(12):1634-9. PMID: 20931137.
    Citations: 12     Fields:    Translation:AnimalsCells
  21. Purschke M, Laubach HJ, Anderson RR, Manstein D. Thermal injury causes DNA damage and lethality in unheated surrounding cells: active thermal bystander effect. J Invest Dermatol. 2010 Jan; 130(1):86-92. PMID: 19587691.
    Citations: 8     Fields:    Translation:HumansCells
  22. Kuhnert VM, Kachnic LA, Li L, Purschke M, Gheorghiu L, Lee R, Held KD, Willers H. FANCD2-deficient human fibroblasts are hypersensitive to ionising radiation at oxygen concentrations of 0% and 3% but not under normoxic conditions. Int J Radiat Biol. 2009 Jun; 85(6):523-31. PMID: 19466639.
    Citations: 6     Fields:    Translation:HumansAnimalsCells
  23. Habbel P, Weylandt KH, Lichopoj K, Nowak J, Purschke M, Wang JD, He CW, Baumgart DC, Kang JX. Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells. World J Gastroenterol. 2009 Mar 07; 15(9):1079-84. PMID: 19266600.
    Citations: 3     Fields:    Translation:HumansCells
  24. Willers H, Kachnic LA, Luo CM, Li L, Purschke M, Borgmann K, Held KD, Powell SN. Biomarkers and mechanisms of FANCD2 function. J Biomed Biotechnol. 2008; 2008:821529. PMID: 18483568.
    Citations: 9     Fields:    Translation:HumansCells
  25. Purschke M, Kasten-Pisula U, Brammer I, Dikomey E. Human and rodent cell lines showing no differences in the induction but differing in the repair kinetics of radiation-induced DNA base damage. Int J Radiat Biol. 2004 Jan; 80(1):29-38. PMID: 14761848.
    Citations: 3     Fields:    Translation:HumansAnimalsCells
  26. Purschke M, Jacobi H, Witte I. Differences in genotoxicity of H(2)O(2) and tetrachlorohydroquinone in human fibroblasts. Mutat Res. 2002 Jan 15; 513(1-2):159-67. PMID: 11719101.
    Citations: 1     Fields:    Translation:HumansCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.