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Alan N. Engelman, Ph.D.

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Mentoring
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Available: 09/23/19, Expires: 09/30/21

HIV integrates into chromosomal DNA as an obligate step in the viral lifecycle, and certain integration sites can influence cellular gene expression and in doing so mold the reservoir of latently infected cells in AIDS patients. It therefore is important to understand the roles that cellular factors play in HIV integration, and the laboratory has over the past several years elucidated roles for the HIV integrase binding protein LEDGF/p75 and capsid binding protein CPSF6 in this process. We since have evidence from a yeast 2-hybrid screen for additional HIV integrase binding proteins, but the roles of these cellular factors in determining sites of HIV integration in the human genome are unknown.

The student will verify novel integrase-host binding interactions in cells using standard biochemistry (co-immunoprecipitation) and cell biology (co-localization by confocal microscopy) techniques. Cell factors that are verified to interact with integrase will be knocked out/ knocked down in cell lines, and HIV will be measured following virus infection by Illumina deep sequencing to map integration sites in the human genome.


Research
research activities and funding
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
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  1. P50GM103368 (OLSON, ARTHUR J.) Sep 1, 2012 - Aug 31, 2017
    NIH/NIGMS
    HIV Macromolecular Interactions and Impact on Viral Evolution of Drug Resistance
    Role: Co-Principal Investigator
  2. R01AI070042 (ENGELMAN, ALAN N.) Apr 1, 2006 - Mar 31, 2021
    NIH/NIAID
    Integrase Structural Virology
    Role: Principal Investigator
  3. R21AI062249 (ENGELMAN, ALAN N.) Jul 1, 2004 - Jun 30, 2006
    NIH/NIAID
    LEDGF-Integrase Structural Biology
    Role: Principal Investigator
  4. R01AI052014 (ENGELMAN, ALAN N.) Jan 15, 2003 - Feb 29, 2012
    NIH/NIAID
    HIV-1 Preintegration Trafficking and Nuclear Localization
    Role: Principal Investigator
  5. R21AI053812 (ENGELMAN, ALAN N.) Sep 20, 2002 - Aug 31, 2004
    NIH/NIAID
    Biochemical Mechanism of HIV DNA Integration
    Role: Principal Investigator

Bibliographic
selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Bester SM, Wei G, Zhao H, Adu-Ampratwum D, Iqbal N, Courouble VV, Francis AC, Annamalai AS, Singh PK, Shkriabai N, Van Blerkom P, Morrison J, Poeschla EM, Engelman AN, Melikyan GB, Griffin PR, Fuchs JR, Asturias FJ, Kvaratskhelia M. Structural and mechanistic bases for a potent HIV-1 capsid inhibitor. Science. 2020 10 16; 370(6514):360-364. PMID: 33060363.
    Citations:    
  2. Li W, Singh PK, Sowd GA, Bedwell GJ, Jang S, Achuthan V, Oleru AV, Wong D, Fadel HJ, Lee K, KewalRamani VN, Poeschla EM, Herschhorn A, Engelman AN. CPSF6-Dependent Targeting of Speckle-Associated Domains Distinguishes Primate from Nonprimate Lentiviral Integration. mBio. 2020 Sep 29; 11(5). PMID: 32994325.
    Citations:    
  3. Elliott JL, Eschbach JE, Koneru PC, Li W, Puray Chavez M, Townsend D, Lawson DQ, Engelman AN, Kvaratskhelia M, Kutluay SB. Integrase-RNA interactions underscore the critical role of integrase in HIV-1 virion morphogenesis. Elife. 2020 Sep 22; 9. PMID: 32960169.
    Citations:    
  4. Jiang C, Lian X, Gao C, Sun X, Einkauf KB, Chevalier JM, Chen SMY, Hua S, Rhee B, Chang K, Blackmer JE, Osborn M, Peluso MJ, Hoh R, Somsouk M, Milush J, Bertagnolli LN, Sweet SE, Varriale JA, Burbelo PD, Chun TW, Laird GM, Serrao E, Engelman AN, Carrington M, Siliciano RF, Siliciano JM, Deeks SG, Walker BD, Lichterfeld M, Yu XG. Distinct viral reservoirs in individuals with spontaneous control of HIV-1. Nature. 2020 09; 585(7824):261-267. PMID: 32848246.
    Citations:    
  5. Ni T, Gerard S, Zhao G, Dent K, Ning J, Zhou J, Shi J, Anderson-Daniels J, Li W, Jang S, Engelman AN, Aiken C, Zhang P. Intrinsic curvature of the HIV-1 CA hexamer underlies capsid topology and interaction with cyclophilin A. Nat Struct Mol Biol. 2020 09; 27(9):855-862. PMID: 32747784.
    Citations:    
  6. Francis AC, Marin M, Singh PK, Achuthan V, Prellberg MJ, Palermino-Rowland K, Lan S, Tedbury PR, Sarafianos SG, Engelman AN, Melikyan GB. HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun. 2020 07 14; 11(1):3505. PMID: 32665593.
    Citations:    
  7. Engelman AN, Cherepanov P. Close-up: HIV/SIV intasome structures shed new light on integrase inhibitor binding and viral escape mechanisms. FEBS J. 2020 Jun 07. PMID: 32506843.
    Citations:    
  8. Chaudhuri E, Dash S, Balasubramaniam M, Padron A, Holland J, Sowd GA, Villalta F, Engelman AN, Pandhare J, Dash C. The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b. J Biol Chem. 2020 Apr 10; 295(15):5081-5094. PMID: 32152226.
    Citations:    
  9. Li M, Chen X, Wang H, Jurado KA, Engelman AN, Craigie R. A Peptide Derived from Lens Epithelium-Derived Growth Factor Stimulates HIV-1 DNA Integration and Facilitates Intasome Structural Studies. J Mol Biol. 2020 03 27; 432(7):2055-2066. PMID: 32061936.
    Citations:    
  10. Cook NJ, Li W, Berta D, Badaoui M, Ballandras-Colas A, Nans A, Kotecha A, Rosta E, Engelman AN, Cherepanov P. Structural basis of second-generation HIV integrase inhibitor action and viral resistance. Science. 2020 02 14; 367(6479):806-810. PMID: 32001525.
    Citations:    
  11. Loyola L, Achuthan V, Gilroy K, Borland G, Kilbey A, Mackay N, Bell M, Hay J, Aiyer S, Fingerman D, Villanueva RA, Cameron E, Kozak CA, Engelman AN, Neil J, Roth MJ. Disrupting MLV integrase:BET protein interaction biases integration into quiescent chromatin and delays but does not eliminate tumor activation in a MYC/Runx2 mouse model. PLoS Pathog. 2019 12; 15(12):e1008154. PMID: 31815961.
    Citations:    
  12. Anderson-Daniels J, Singh PK, Sowd GA, Li W, Engelman AN, Aiken C. Dominant Negative MA-CA Fusion Protein Is Incorporated into HIV-1 Cores and Inhibits Nuclear Entry of Viral Preintegration Complexes. J Virol. 2019 11 01; 93(21). PMID: 31413124.
    Citations:    
  13. Engelman AN. Multifaceted HIV integrase functionalities and therapeutic strategies for their inhibition. J Biol Chem. 2019 10 11; 294(41):15137-15157. PMID: 31467082.
    Citations:    
  14. Zhang DW, Luo RH, Xu L, Yang LM, Xu XS, Bedwell GJ, Engelman AN, Zheng YT, Chang S. A HTRF based competitive binding assay for screening specific inhibitors of HIV-1 capsid assembly targeting the C-Terminal domain of capsid. Antiviral Res. 2019 09; 169:104544. PMID: 31254557.
    Citations:    
  15. Achuthan V, Perreira JM, Ahn JJ, Brass AL, Engelman AN. Capsid-CPSF6 interaction: Master regulator of nuclear HIV-1 positioning and integration. J Life Sci (Westlake Village). 2019 Jun; 1(1):39-45. PMID: 31448372.
    Citations:    
  16. Koneru PC, Francis AC, Deng N, Rebensburg SV, Hoyte AC, Lindenberger J, Adu-Ampratwum D, Larue RC, Wempe MF, Engelman AN, Lyumkis D, Fuchs JR, Levy RM, Melikyan GB, Kvaratskhelia M. HIV-1 integrase tetramers are the antiviral target of pyridine-based allosteric integrase inhibitors. Elife. 2019 05 23; 8. PMID: 31120420.
    Citations:    
  17. Jang S, Cook NJ, Pye VE, Bedwell GJ, Dudek AM, Singh PK, Cherepanov P, Engelman AN. Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6. Nucleic Acids Res. 2019 05 21; 47(9):4663-4683. PMID: 30916345.
    Citations:    
  18. Achuthan V, Perreira JM, Sowd GA, Puray-Chavez M, McDougall WM, Paulucci-Holthauzen A, Wu X, Fadel HJ, Poeschla EM, Multani AS, Hughes SH, Sarafianos SG, Brass AL, Engelman AN. Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA Integration. . 2018 09 12; 24(3):392-404.e8. PMID: 30173955.
    Citations:    Fields:    
  19. Engelman AN, Singh PK. Cellular and molecular mechanisms of HIV-1 integration targeting. Cell Mol Life Sci. 2018 Jul; 75(14):2491-2507. PMID: 29417178.
    Citations: 1     Fields:    Translation:HumansCells
  20. Zhu Y, Wang X, Forouzmand E, Jeong J, Qiao F, Sowd GA, Engelman AN, Xie X, Hertel KJ, Shi Y. Molecular Mechanisms for CFIm-Mediated Regulation of mRNA Alternative Polyadenylation. Mol Cell. 2018 01 04; 69(1):62-74.e4. PMID: 29276085.
    Citations: 1     Fields:    Translation:HumansAnimalsCells
  21. Puray-Chavez M, Tedbury PR, Huber AD, Ukah OB, Yapo V, Liu D, Ji J, Wolf JJ, Engelman AN, Sarafianos SG. Multiplex single-cell visualization of nucleic acids and protein during HIV infection. Nat Commun. 2017 12 01; 8(1):1882. PMID: 29192235.
    Citations: 4     Fields:    Translation:HumansCells
  22. El Ashkar S, Schwaller J, Pieters T, Goossens S, Demeulemeester J, Christ F, Van Belle S, Juge S, Boeckx N, Engelman A, Van Vlierberghe P, Debyser Z, De Rijck J. LEDGF/p75 is dispensable for hematopoiesis but essential for MLL-rearranged leukemogenesis. Blood. 2018 01 04; 131(1):95-107. PMID: 29084774.
    Citations: 1     Fields:    Translation:AnimalsCells
  23. Hoyte AC, Jamin AV, Koneru PC, Kobe MJ, Larue RC, Fuchs JR, Engelman AN, Kvaratskhelia M. Resistance to pyridine-based inhibitor KF116 reveals an unexpected role of integrase in HIV-1 Gag-Pol polyprotein proteolytic processing. J Biol Chem. 2017 12 01; 292(48):19814-19825. PMID: 28972144.
    Citations: 1     Fields:    Translation:HumansCells
  24. Alvarez FJD, He S, Perilla JR, Jang S, Schulten K, Engelman AN, Scheres SHW, Zhang P. CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction. Sci Adv. 2017 09; 3(9):e1701264. PMID: 28929138.
    Citations: 2     Fields:    
  25. Yamashita M, Engelman AN. Capsid-Dependent Host Factors in HIV-1 Infection. Trends Microbiol. 2017 09; 25(9):741-755. PMID: 28528781.
    Citations: 5     Fields:    Translation:HumansCells
  26. Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley GQ. Haematopoietic stem and progenitor cells from human pluripotent stem cells. Nature. 2017 05 25; 545(7655):432-438. PMID: 28514439.
    Citations: 37     Fields:    Translation:HumansAnimalsCells
  27. Lesbats P, Serrao E, Maskell DP, Pye VE, O'Reilly N, Lindemann D, Engelman AN, Cherepanov P. Structural basis for spumavirus GAG tethering to chromatin. Proc Natl Acad Sci U S A. 2017 05 23; 114(21):5509-5514. PMID: 28490494.
    Citations: 1     Fields:    Translation:Cells
  28. Engelman AN, Cherepanov P. Retroviral intasomes arising. Curr Opin Struct Biol. 2017 12; 47:23-29. PMID: 28458055.
    Citations: 3     Fields:    Translation:Cells
  29. Wang W, Zhou J, Halambage UD, Jurado KA, Jamin AV, Wang Y, Engelman AN, Aiken C. Inhibition of HIV-1 Maturation via Small-Molecule Targeting of the Amino-Terminal Domain in the Viral Capsid Protein. J Virol. 2017 05 01; 91(9). PMID: 28202766.
    Citations: 3     Fields:    Translation:HumansCells
  30. Grawenhoff J, Engelman AN. Retroviral integrase protein and intasome nucleoprotein complex structures. World J Biol Chem. 2017 Feb 26; 8(1):32-44. PMID: 28289517.
    Citations: 2     
  31. Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jónsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andrésdóttir V, Engelman AN, Costa A, Cherepanov P. A supramolecular assembly mediates lentiviral DNA integration. Science. 2017 01 06; 355(6320):93-95. PMID: 28059770.
    Citations: 14     Fields:    Translation:HumansCells
  32. Lu YF, Cahan P, Ross S, Sahalie J, Sousa PM, Hadland BK, Cai W, Serrao E, Engelman AN, Bernstein ID, Daley GQ. Engineered Murine HSCs Reconstitute Multi-lineage Hematopoiesis and Adaptive Immunity. Cell Rep. 2016 12 20; 17(12):3178-3192. PMID: 28009288.
    Citations: 2     Fields:    Translation:HumansAnimalsCells
  33. Zurnic I, Hütter S, Rzeha U, Helbig R, Stanke N, Reh J, Müllers E, Hamann MV, Kern T, Gerresheim GK, Lindel F, Serrao E, Lesbats P, Engelman AN, Cherepanov P, Lindemann D. Correction: Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration. PLoS Pathog. 2016 Oct; 12(10):e1005956. PMID: 27723818.
    Citations:    Fields:    
  34. Patel D, Antwi J, Koneru PC, Serrao E, Forli S, Kessl JJ, Feng L, Deng N, Levy RM, Fuchs JR, Olson AJ, Engelman AN, Bauman JD, Kvaratskhelia M, Arnold E. A New Class of Allosteric HIV-1 Integrase Inhibitors Identified by Crystallographic Fragment Screening of the Catalytic Core Domain. J Biol Chem. 2016 Nov 04; 291(45):23569-23577. PMID: 27645997.
    Citations: 2     Fields:    Translation:HumansCells
  35. Zurnic I, Hütter S, Rzeha U, Stanke N, Reh J, Müllers E, Hamann MV, Kern T, Gerresheim GK, Lindel F, Serrao E, Lesbats P, Engelman AN, Cherepanov P, Lindemann D. Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration. PLoS Pathog. 2016 08; 12(8):e1005860. PMID: 27579920.
    Citations: 3     Fields:    Translation:HumansAnimalsCells
  36. Zerbato JM, Serrao E, Lenzi G, Kim B, Ambrose Z, Watkins SC, Engelman AN, Sluis-Cremer N. Establishment and Reversal of HIV-1 Latency in Naive and Central Memory CD4+ T Cells In Vitro. J Virol. 2016 09 15; 90(18):8059-73. PMID: 27356901.
    Citations: 5     Fields:    Translation:HumansCells
  37. Deng N, Hoyte A, Mansour YE, Mohamed MS, Fuchs JR, Engelman AN, Kvaratskhelia M, Levy R. Allosteric HIV-1 integrase inhibitors promote aberrant protein multimerization by directly mediating inter-subunit interactions: Structural and thermodynamic modeling studies. Protein Sci. 2016 11; 25(11):1911-1917. PMID: 27503276.
    Citations: 3     Fields:    Translation:Cells
  38. Saito A, Henning MS, Serrao E, Dubose BN, Teng S, Huang J, Li X, Saito N, Roy SP, Siddiqui MA, Ahn J, Tsuji M, Hatziioannou T, Engelman AN, Yamashita M. Capsid-CPSF6 Interaction Is Dispensable for HIV-1 Replication in Primary Cells but Is Selected during Virus Passage In Vivo. J Virol. 2016 08 01; 90(15):6918-6935. PMID: 27307565.
    Citations: 6     Fields:    Translation:HumansAnimalsCells
  39. Saito A, Ferhadian D, Sowd GA, Serrao E, Shi J, Halambage UD, Teng S, Soto J, Siddiqui MA, Engelman AN, Aiken C, Yamashita M. Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74. J Virol. 2016 06 15; 90(12):5808-5823. PMID: 27076642.
    Citations: 9     Fields:    Translation:HumansCells
  40. Lesbats P, Engelman AN, Cherepanov P. Retroviral DNA Integration. Chem Rev. 2016 10 26; 116(20):12730-12757. PMID: 27198982.
    Citations: 23     Fields:    Translation:Cells
  41. Serrao E, Cherepanov P, Engelman AN. Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites. J Vis Exp. 2016 Mar 22; (109). PMID: 27023428.
    Citations: 5     Fields:    Translation:HumansCells
  42. Rasheedi S, Shun MC, Serrao E, Sowd GA, Qian J, Hao C, Dasgupta T, Engelman AN, Skowronski J. The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes. J Biol Chem. 2016 May 27; 291(22):11809-19. PMID: 26994143.
    Citations: 9     Fields:    Translation:HumansCells
  43. Feng L, Dharmarajan V, Serrao E, Hoyte A, Larue RC, Slaughter A, Sharma A, Plumb MR, Kessl JJ, Fuchs JR, Bushman FD, Engelman AN, Griffin PR, Kvaratskhelia M. The Competitive Interplay between Allosteric HIV-1 Integrase Inhibitor BI/D and LEDGF/p75 during the Early Stage of HIV-1 Replication Adversely Affects Inhibitor Potency. ACS Chem Biol. 2016 05 20; 11(5):1313-21. PMID: 26910179.
    Citations: 6     Fields:    Translation:HumansCells
  44. Ballandras-Colas A, Brown M, Cook NJ, Dewdney TG, Demeler B, Cherepanov P, Lyumkis D, Engelman AN. Cryo-EM reveals a novel octameric integrase structure for betaretroviral intasome function. Nature. 2016 Feb 18; 530(7590):358-61. PMID: 26887496.
    Citations: 25     Fields:    Translation:Cells
  45. Sowd GA, Serrao E, Wang H, Wang W, Fadel HJ, Poeschla EM, Engelman AN. A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin. Proc Natl Acad Sci U S A. 2016 Feb 23; 113(8):E1054-63. PMID: 26858452.
    Citations: 31     Fields:    Translation:HumansCells
  46. Serrao E, Engelman AN. Sites of retroviral DNA integration: From basic research to clinical applications. Crit Rev Biochem Mol Biol. 2016; 51(1):26-42. PMID: 26508664.
    Citations: 7     Fields:    Translation:Cells
  47. Nuñez JK, Harrington LB, Kranzusch PJ, Engelman AN, Doudna JA. Foreign DNA capture during CRISPR-Cas adaptive immunity. Nature. 2015 Nov 26; 527(7579):535-8. PMID: 26503043.
    Citations: 39     Fields:    Translation:Cells
  48. Wang H, Shun MC, Dickson AK, Engelman AN. Embryonic Lethality Due to Arrested Cardiac Development in Psip1/Hdgfrp2 Double-Deficient Mice. PLoS One. 2015; 10(9):e0137797. PMID: 26367869.
    Citations: 4     Fields:    Translation:Animals
  49. Fontana J, Jurado KA, Cheng N, Ly NL, Fuchs JR, Gorelick RJ, Engelman AN, Steven AC. Distribution and Redistribution of HIV-1 Nucleocapsid Protein in Immature, Mature, and Integrase-Inhibited Virions: a Role for Integrase in Maturation. J Virol. 2015 Oct; 89(19):9765-80. PMID: 26178982.
    Citations: 21     Fields:    Translation:HumansCells
  50. Maskell DP, Renault L, Serrao E, Lesbats P, Matadeen R, Hare S, Lindemann D, Engelman AN, Costa A, Cherepanov P. Structural basis for retroviral integration into nucleosomes. Nature. 2015 Jul 16; 523(7560):366-9. PMID: 26061770.
    Citations: 41     Fields:    Translation:Cells
  51. Serrao E, Ballandras-Colas A, Cherepanov P, Maertens GN, Engelman AN. Key determinants of target DNA recognition by retroviral intasomes. Retrovirology. 2015 Apr 30; 12:39. PMID: 25924943.
    Citations: 19     Fields:    Translation:HumansCells
  52. Nuñez JK, Lee AS, Engelman A, Doudna JA. Integrase-mediated spacer acquisition during CRISPR-Cas adaptive immunity. Nature. 2015 Mar 12; 519(7542):193-8. PMID: 25707795.
    Citations: 71     Fields:    Translation:Cells
  53. Engelman A, Cherepanov P. Retroviral Integrase Structure and DNA Recombination Mechanism. Microbiol Spectr. 2014 Dec; 2(6). PMID: 26104441.
    Citations: 4     Fields:    Translation:Cells
  54. Slaughter A, Jurado KA, Deng N, Feng L, Kessl JJ, Shkriabai N, Larue RC, Fadel HJ, Patel PA, Jena N, Fuchs JR, Poeschla E, Levy RM, Engelman A, Kvaratskhelia M. The mechanism of H171T resistance reveals the importance of Nd-protonated His171 for the binding of allosteric inhibitor BI-D to HIV-1 integrase. Retrovirology. 2014 Nov 25; 11:100. PMID: 25421939.
    Citations: 13     Fields:    Translation:HumansCells
  55. Matreyek KA, Wang W, Serrao E, Singh PK, Levin HL, Engelman A. Host and viral determinants for MxB restriction of HIV-1 infection. Retrovirology. 2014 Oct 25; 11:90. PMID: 25348155.
    Citations: 28     Fields:    Translation:HumansCells
  56. Fribourgh JL, Nguyen HC, Matreyek KA, Alvarez FJD, Summers BJ, Dewdney TG, Aiken C, Zhang P, Engelman A, Xiong Y. Structural insight into HIV-1 restriction by MxB. . 2014 Nov 12; 16(5):627-638. PMID: 25312384.
    Citations: 26     Fields:    Translation:HumansCells
  57. Kvaratskhelia M, Sharma A, Larue RC, Serrao E, Engelman A. Molecular mechanisms of retroviral integration site selection. Nucleic Acids Res. 2014; 42(16):10209-25. PMID: 25147212.
    Citations: 29     Fields:    Translation:HumansCells
  58. Li M, Jurado KA, Lin S, Engelman A, Craigie R. Engineered hyperactive integrase for concerted HIV-1 DNA integration. PLoS One. 2014; 9(8):e105078. PMID: 25119883.
    Citations: 9     Fields:    Translation:HumansCells
  59. Serrao E, Krishnan L, Shun MC, Li X, Cherepanov P, Engelman A, Maertens GN. Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding. Nucleic Acids Res. 2014 Apr; 42(8):5164-76. PMID: 24520116.
    Citations: 24     Fields:    Translation:Cells
  60. Maertens GN, Cook NJ, Wang W, Hare S, Gupta SS, Öztop I, Lee K, Pye VE, Cosnefroy O, Snijders AP, KewalRamani VN, Fassati A, Engelman A, Cherepanov P. Structural basis for nuclear import of splicing factors by human Transportin 3. Proc Natl Acad Sci U S A. 2014 Feb 18; 111(7):2728-33. PMID: 24449914.
    Citations: 25     Fields:    Translation:HumansCells
  61. Wang H, Shun MC, Li X, Di Nunzio F, Hare S, Cherepanov P, Engelman A. Efficient Transduction of LEDGF/p75 Mutant Cells by Gain-of-Function HIV-1 Integrase Mutant Viruses. Mol Ther Methods Clin Dev. 2014 Jan 08; 1. PMID: 25383358.
    Citations: 6     
  62. Engelman A, Cherepanov P. Retroviral Integrase Structure and DNA Recombination Mechanism. Microbiol Spectr. 2014; 2(6):1-22. PMID: 25705574.
    Citations: 14     Fields:    
  63. Jurado KA, Engelman A. Multimodal mechanism of action of allosteric HIV-1 integrase inhibitors. Expert Rev Mol Med. 2013 Nov 26; 15:e14. PMID: 24274067.
    Citations: 20     Fields:    Translation:HumansAnimalsCells
  64. Matreyek KA, Yücel SS, Li X, Engelman A. Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity. PLoS Pathog. 2013; 9(10):e1003693. PMID: 24130490.
    Citations: 58     Fields:    Translation:HumansCells
  65. Matreyek KA, Engelman A. Viral and cellular requirements for the nuclear entry of retroviral preintegration nucleoprotein complexes. Viruses. 2013 Oct 07; 5(10):2483-511. PMID: 24103892.
    Citations: 38     Fields:    Translation:HumansCells
  66. Ballandras-Colas A, Naraharisetty H, Li X, Serrao E, Engelman A. Biochemical characterization of novel retroviral integrase proteins. PLoS One. 2013; 8(10):e76638. PMID: 24124581.
    Citations: 11     Fields:    Translation:HumansAnimalsCells
  67. Engelman A, Kessl JJ, Kvaratskhelia M. Allosteric inhibition of HIV-1 integrase activity. Curr Opin Chem Biol. 2013 Jun; 17(3):339-45. PMID: 23647983.
    Citations: 25     Fields:    Translation:HumansCells
  68. Feng L, Sharma A, Slaughter A, Jena N, Koh Y, Shkriabai N, Larue RC, Patel PA, Mitsuya H, Kessl JJ, Engelman A, Fuchs JR, Kvaratskhelia M. The A128T resistance mutation reveals aberrant protein multimerization as the primary mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem. 2013 May 31; 288(22):15813-20. PMID: 23615903.
    Citations: 29     Fields:    Translation:HumansCells
  69. Jurado KA, Wang H, Slaughter A, Feng L, Kessl JJ, Koh Y, Wang W, Ballandras-Colas A, Patel PA, Fuchs JR, Kvaratskhelia M, Engelman A. Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation. Proc Natl Acad Sci U S A. 2013 May 21; 110(21):8690-5. PMID: 23610442.
    Citations: 60     Fields:    Translation:HumansCells
  70. Koh Y, Ballana E, Este J, Engelman A. Polymorphic LEDGF/p75 variants support efficient HIV-1 infection ex vivo. AIDS. 2013 Feb 20; 27(4):665-7. PMID: 23364444.
    Citations: 2     Fields:    Translation:HumansAnimalsCells
  71. Koh Y, Wu X, Ferris AL, Matreyek KA, Smith SJ, Lee K, KewalRamani VN, Hughes SH, Engelman A. Differential effects of human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity of viral DNA integration. J Virol. 2013 Jan; 87(1):648-58. PMID: 23097450.
    Citations: 40     Fields:    Translation:HumansAnimalsCells
  72. Shah VB, Shi J, Hout DR, Oztop I, Krishnan L, Ahn J, Shotwell MS, Engelman A, Aiken C. The host proteins transportin SR2/TNPO3 and cyclophilin A exert opposing effects on HIV-1 uncoating. J Virol. 2013 Jan; 87(1):422-32. PMID: 23097435.
    Citations: 49     Fields:    Translation:HumansCells
  73. Wang H, Jurado KA, Wu X, Shun MC, Li X, Ferris AL, Smith SJ, Patel PA, Fuchs JR, Cherepanov P, Kvaratskhelia M, Hughes SH, Engelman A. HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor. Nucleic Acids Res. 2012 Dec; 40(22):11518-30. PMID: 23042676.
    Citations: 34     Fields:    Translation:HumansAnimalsCells
  74. Krishnan L, Engelman A. Retroviral integrase proteins and HIV-1 DNA integration. J Biol Chem. 2012 Nov 30; 287(49):40858-66. PMID: 23043109.
    Citations: 31     Fields:    Translation:HumansCells
  75. Matreyek KA, Oztop I, Freed EO, Engelman A. Viral latency and potential eradication of HIV-1. Expert Rev Anti Infect Ther. 2012 Aug; 10(8):855-7. PMID: 23030323.
    Citations: 3     Fields:    Translation:HumansAnimalsCells
  76. Kessl JJ, Jena N, Koh Y, Taskent-Sezgin H, Slaughter A, Feng L, de Silva S, Wu L, Le Grice SF, Engelman A, Fuchs JR, Kvaratskhelia M. Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem. 2012 May 11; 287(20):16801-11. PMID: 22437836.
    Citations: 62     Fields:    Translation:HumansCells
  77. Engelman A, Cherepanov P. The structural biology of HIV-1: mechanistic and therapeutic insights. Nat Rev Microbiol. 2012 03 16; 10(4):279-90. PMID: 22421880.
    Citations: 56     Fields:    Translation:HumansCells
  78. Ambrose Z, Lee K, Ndjomou J, Xu H, Oztop I, Matous J, Takemura T, Unutmaz D, Engelman A, Hughes SH, KewalRamani VN. Human immunodeficiency virus type 1 capsid mutation N74D alters cyclophilin A dependence and impairs macrophage infection. J Virol. 2012 Apr; 86(8):4708-14. PMID: 22301145.
    Citations: 42     Fields:    Translation:HumansAnimalsCells
  79. Li X, Koh Y, Engelman A. Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus. J Virol. 2012 Apr; 86(7):3861-79. PMID: 22278243.
    Citations: 13     Fields:    Translation:HumansCells
  80. Matreyek KA, Engelman A. The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid. J Virol. 2011 Aug; 85(15):7818-27. PMID: 21593146.
    Citations: 78     Fields:    Translation:HumansCells
  81. Yan N, O'Day E, Wheeler LA, Engelman A, Lieberman J. HIV DNA is heavily uracilated, which protects it from autointegration. Proc Natl Acad Sci U S A. 2011 May 31; 108(22):9244-9. PMID: 21576478.
    Citations: 28     Fields:    Translation:HumansCells
  82. Zamborlini A, Coiffic A, Beauclair G, Delelis O, Paris J, Koh Y, Magne F, Giron ML, Tobaly-Tapiero J, Deprez E, Emiliani S, Engelman A, de Thé H, Saïb A. Impairment of human immunodeficiency virus type-1 integrase SUMOylation correlates with an early replication defect. J Biol Chem. 2011 Jun 10; 286(23):21013-22. PMID: 21454548.
    Citations: 21     Fields:    Translation:HumansCells
  83. Koh Y, Matreyek KA, Engelman A. Differential sensitivities of retroviruses to integrase strand transfer inhibitors. J Virol. 2011 Apr; 85(7):3677-82. PMID: 21270168.
    Citations: 13     Fields:    Translation:HumansAnimalsCells
  84. Li X, Krishnan L, Cherepanov P, Engelman A. Structural biology of retroviral DNA integration. Virology. 2011 Mar 15; 411(2):194-205. PMID: 21216426.
    Citations: 52     Fields:    Translation:HumansAnimalsCells
  85. Koh Y, Haim H, Engelman A. Identification and characterization of persistent intracellular human immunodeficiency virus type 1 integrase strand transfer inhibitor activity. Antimicrob Agents Chemother. 2011 Jan; 55(1):42-9. PMID: 21060108.
    Citations: 10     Fields:    Translation:HumansCells
  86. Krishnan L, Li X, Naraharisetty HL, Hare S, Cherepanov P, Engelman A. Structure-based modeling of the functional HIV-1 intasome and its inhibition. Proc Natl Acad Sci U S A. 2010 Sep 07; 107(36):15910-5. PMID: 20733078.
    Citations: 88     Fields:    Translation:HumansCells
  87. Lee K, Ambrose Z, Martin TD, Oztop I, Mulky A, Julias JG, Vandegraaff N, Baumann JG, Wang R, Yuen W, Takemura T, Shelton K, Taniuchi I, Li Y, Sodroski J, Littman DR, Coffin JM, Hughes SH, Unutmaz D, Engelman A, KewalRamani VN. Flexible use of nuclear import pathways by HIV-1. . 2010 Mar 18; 7(3):221-33. PMID: 20227665.
    Citations: 166     Fields:    Translation:HumansAnimalsCells
  88. Ferris AL, Wu X, Hughes CM, Stewart C, Smith SJ, Milne TA, Wang GG, Shun MC, Allis CD, Engelman A, Hughes SH. Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration. Proc Natl Acad Sci U S A. 2010 Feb 16; 107(7):3135-40. PMID: 20133638.
    Citations: 72     Fields:    Translation:AnimalsCells
  89. Hare S, Gupta SS, Valkov E, Engelman A, Cherepanov P. Retroviral intasome assembly and inhibition of DNA strand transfer. Nature. 2010 Mar 11; 464(7286):232-6. PMID: 20118915.
    Citations: 243     Fields:    Translation:Cells
  90. Hughes S, Jenkins V, Dar MJ, Engelman A, Cherepanov P. Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem. 2010 Jan 01; 285(1):541-54. PMID: 19864417.
    Citations: 28     Fields:    Translation:HumansCells
  91. Krishnan L, Matreyek KA, Oztop I, Lee K, Tipper CH, Li X, Dar MJ, Kewalramani VN, Engelman A. The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase. J Virol. 2010 Jan; 84(1):397-406. PMID: 19846519.
    Citations: 97     Fields:    Translation:HumansAnimalsCells
  92. Dar MJ, Monel B, Krishnan L, Shun MC, Di Nunzio F, Helland DE, Engelman A. Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase. Retrovirology. 2009 Oct 19; 6:94. PMID: 19840380.
    Citations: 19     Fields:    Translation:Cells
  93. Hare S, Di Nunzio F, Labeja A, Wang J, Engelman A, Cherepanov P. Structural basis for functional tetramerization of lentiviral integrase. PLoS Pathog. 2009 Jul; 5(7):e1000515. PMID: 19609359.
    Citations: 57     Fields:    Translation:Cells
  94. Engelman A. Mechanistic and pharmacological analyses of HIV-1 integration. Methods. 2009 Apr; 47(4):225-8. PMID: 19389610.
    Citations:    Fields:    Translation:HumansAnimalsCells
  95. Belshan M, Schweitzer CJ, Donnellan MR, Lu R, Engelman A. In vivo biotinylation and capture of HIV-1 matrix and integrase proteins. J Virol Methods. 2009 Aug; 159(2):178-84. PMID: 19490971.
    Citations: 11     Fields:    Translation:HumansCells
  96. Yan N, Cherepanov P, Daigle JE, Engelman A, Lieberman J. The SET complex acts as a barrier to autointegration of HIV-1. PLoS Pathog. 2009 Mar; 5(3):e1000327. PMID: 19266025.
    Citations: 40     Fields:    Translation:HumansAnimalsCells
  97. Engelman A, Oztop I, Vandegraaff N, Raghavendra NK. Quantitative analysis of HIV-1 preintegration complexes. Methods. 2009 Apr; 47(4):283-90. PMID: 19233280.
    Citations: 13     Fields:    Translation:HumansAnimalsCells
  98. Hare S, Shun MC, Gupta SS, Valkov E, Engelman A, Cherepanov P. A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75. PLoS Pathog. 2009 Jan; 5(1):e1000259. PMID: 19132083.
    Citations: 78     Fields:    Translation:HumansCells
  99. Engelman A. Isolation and analysis of HIV-1 preintegration complexes. Methods Mol Biol. 2009; 485:135-49. PMID: 19020823.
    Citations: 6     Fields:    Translation:HumansCells
  100. McKee CJ, Kessl JJ, Shkriabai N, Dar MJ, Engelman A, Kvaratskhelia M. Dynamic modulation of HIV-1 integrase structure and function by cellular lens epithelium-derived growth factor (LEDGF) protein. J Biol Chem. 2008 Nov 14; 283(46):31802-12. PMID: 18801737.
    Citations: 60     Fields:    Translation:Cells
  101. Shun MC, Botbol Y, Li X, Di Nunzio F, Daigle JE, Yan N, Lieberman J, Lavigne M, Engelman A. Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. J Virol. 2008 Dec; 82(23):11555-67. PMID: 18799576.
    Citations: 36     Fields:    Translation:HumansAnimalsCells
  102. Engelman A, Cherepanov P. The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. PLoS Pathog. 2008 Mar 28; 4(3):e1000046. PMID: 18369482.
    Citations: 103     Fields:    Translation:Cells
  103. Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ. Identification of host proteins required for HIV infection through a functional genomic screen. Science. 2008 Feb 15; 319(5865):921-6. PMID: 18187620.
    Citations: 642     Fields:    Translation:HumansCells
  104. Botbol Y, Raghavendra NK, Rahman S, Engelman A, Lavigne M. Chromatinized templates reveal the requirement for the LEDGF/p75 PWWP domain during HIV-1 integration in vitro. Nucleic Acids Res. 2008 Mar; 36(4):1237-46. PMID: 18174227.
    Citations: 27     Fields:    Translation:HumansCells
  105. Zhao Z, McKee CJ, Kessl JJ, Santos WL, Daigle JE, Engelman A, Verdine G, Kvaratskhelia M. Subunit-specific protein footprinting reveals significant structural rearrangements and a role for N-terminal Lys-14 of HIV-1 Integrase during viral DNA binding. J Biol Chem. 2008 Feb 29; 283(9):5632-41. PMID: 18093980.
    Citations: 34     Fields:    Translation:HumansCells
  106. Shun MC, Raghavendra NK, Vandegraaff N, Daigle JE, Hughes S, Kellam P, Cherepanov P, Engelman A. LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev. 2007 Jul 15; 21(14):1767-78. PMID: 17639082.
    Citations: 211     Fields:    Translation:HumansAnimalsCells
  107. Engelman A. AIDS/HIV. A reversal of fortune in HIV-1 integration. Science. 2007 Jun 29; 316(5833):1855-7. PMID: 17600205.
    Citations: 2     Fields:    Translation:HumansCells
  108. Mbisa JL, Barr R, Thomas JA, Vandegraaff N, Dorweiler IJ, Svarovskaia ES, Brown WL, Mansky LM, Gorelick RJ, Harris RS, Engelman A, Pathak VK. Human immunodeficiency virus type 1 cDNAs produced in the presence of APOBEC3G exhibit defects in plus-strand DNA transfer and integration. J Virol. 2007 Jul; 81(13):7099-110. PMID: 17428871.
    Citations: 169     Fields:    Translation:HumansCells
  109. Vandegraaff N, Engelman A. Molecular mechanisms of HIV integration and therapeutic intervention. Expert Rev Mol Med. 2007 Feb 26; 9(6):1-19. PMID: 17320002.
    Citations: 25     Fields:    Translation:HumansAnimalsCells
  110. Daelemans D, Lu R, De Clercq E, Engelman A. Characterization of a replication-competent, integrase-defective human immunodeficiency virus (HIV)/simian virus 40 chimera as a powerful tool for the discovery and validation of HIV integrase inhibitors. J Virol. 2007 Apr; 81(8):4381-5. PMID: 17287285.
    Citations: 5     Fields:    Translation:HumansCells
  111. Raghavendra NK, Engelman A. LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration. Virology. 2007 Mar 30; 360(1):1-5. PMID: 17258258.
    Citations: 22     Fields:    Translation:HumansCells
  112. Shun MC, Daigle JE, Vandegraaff N, Engelman A. Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein. J Virol. 2007 Jan; 81(1):166-72. PMID: 17035312.
    Citations: 34     Fields:    Translation:HumansAnimalsCells
  113. Anderson JL, Campbell EM, Wu X, Vandegraaff N, Engelman A, Hope TJ. Proteasome inhibition reveals that a functional preintegration complex intermediate can be generated during restriction by diverse TRIM5 proteins. J Virol. 2006 Oct; 80(19):9754-60. PMID: 16973579.
    Citations: 96     Fields:    Translation:HumansAnimalsCells
  114. Rahman S, Lu R, Vandegraaff N, Cherepanov P, Engelman A. Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness. Virology. 2007 Jan 05; 357(1):79-90. PMID: 16959283.
    Citations: 28     Fields:    Translation:HumansCells
  115. Maertens GN, Cherepanov P, Engelman A. Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin. J Cell Sci. 2006 Jun 15; 119(Pt 12):2563-71. PMID: 16735438.
    Citations: 62     Fields:    Translation:HumansCells
  116. Diaz-Griffero F, Vandegraaff N, Li Y, McGee-Estrada K, Stremlau M, Welikala S, Si Z, Engelman A, Sodroski J. Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1. Virology. 2006 Aug 01; 351(2):404-19. PMID: 16650449.
    Citations: 52     Fields:    Translation:HumansAnimalsCells
  117. Si Z, Vandegraaff N, O'huigin C, Song B, Yuan W, Xu C, Perron M, Li X, Marasco WA, Engelman A, Dean M, Sodroski J. Evolution of a cytoplasmic tripartite motif (TRIM) protein in cows that restricts retroviral infection. Proc Natl Acad Sci U S A. 2006 May 09; 103(19):7454-9. PMID: 16648259.
    Citations: 48     Fields:    Translation:AnimalsCells
  118. Turlure F, Maertens G, Rahman S, Cherepanov P, Engelman A. A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. Nucleic Acids Res. 2006; 34(5):1653-65. PMID: 16549878.
    Citations: 81     Fields:    Translation:HumansCells
  119. Vandegraaff N, Devroe E, Turlure F, Silver PA, Engelman A. Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication. Virology. 2006 Mar 15; 346(2):415-26. PMID: 16337983.
    Citations: 53     Fields:    Translation:HumansCells
  120. Cherepanov P, Ambrosio AL, Rahman S, Ellenberger T, Engelman A. Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75. Proc Natl Acad Sci U S A. 2005 Nov 29; 102(48):17308-13. PMID: 16260736.
    Citations: 159     Fields:    Translation:HumansCells
  121. Lu R, Vandegraaff N, Cherepanov P, Engelman A. Lys-34, dispensable for integrase catalysis, is required for preintegration complex function and human immunodeficiency virus type 1 replication. J Virol. 2005 Oct; 79(19):12584-91. PMID: 16160186.
    Citations: 16     Fields:    Translation:Cells
  122. Lu R, Ghory HZ, Engelman A. Genetic analyses of conserved residues in the carboxyl-terminal domain of human immunodeficiency virus type 1 integrase. J Virol. 2005 Aug; 79(16):10356-68. PMID: 16051828.
    Citations: 38     Fields:    Translation:HumansCells
  123. Cherepanov P, Sun ZY, Rahman S, Maertens G, Wagner G, Engelman A. Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75. Nat Struct Mol Biol. 2005 Jun; 12(6):526-32. PMID: 15895093.
    Citations: 93     Fields:    Translation:HumansAnimalsCells
  124. Lu R, Limón A, Ghory HZ, Engelman A. Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase. J Virol. 2005 Feb; 79(4):2493-505. PMID: 15681450.
    Citations: 37     Fields:    Translation:HumansCells
  125. Engelman A. The ups and downs of gene expression and retroviral DNA integration. Proc Natl Acad Sci U S A. 2005 Feb 01; 102(5):1275-6. PMID: 15677323.
    Citations: 14     Fields:    Translation:Cells
  126. Devroe E, Silver PA, Engelman A. HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases. Virology. 2005 Jan 05; 331(1):181-9. PMID: 15582665.
    Citations: 13     Fields:    Translation:HumansCells
  127. Lu R, Limón A, Devroe E, Silver PA, Cherepanov P, Engelman A. Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. J Virol. 2004 Dec; 78(23):12735-46. PMID: 15542626.
    Citations: 65     Fields:    Translation:HumansCells
  128. Cherepanov P, Devroe E, Silver PA, Engelman A. Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. J Biol Chem. 2004 Nov 19; 279(47):48883-92. PMID: 15371438.
    Citations: 113     Fields:    Translation:HumansAnimalsCells
  129. Turlure F, Devroe E, Silver PA, Engelman A. Human cell proteins and human immunodeficiency virus DNA integration. Front Biosci. 2004 Sep 01; 9:3187-208. PMID: 15353349.
    Citations: 74     Fields:    Translation:HumansAnimalsCells
  130. Maertens G, Cherepanov P, Debyser Z, Engelborghs Y, Engelman A. Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75. J Biol Chem. 2004 Aug 06; 279(32):33421-9. PMID: 15163664.
    Citations: 38     Fields:    Translation:HumansCells
  131. Byers KB, Engelman A, Fontes B. General guidelines for experimenting with HIV. Curr Protoc Immunol. 2004 May; Chapter 12:Unit 12.1. PMID: 18432923.
    Citations:    Fields:    Translation:HumansAnimalsCells
  132. Lu R, Nakajima N, Hofmann W, Benkirane M, Jeang KT, Sodroski J, Engelman A, Teh-Jeang K. Simian virus 40-based replication of catalytically inactive human immunodeficiency virus type 1 integrase mutants in nonpermissive T cells and monocyte-derived macrophages. J Virol. 2004 Jan; 78(2):658-68. PMID: 14694097.
    Citations: 14     Fields:    Translation:HumansCells
  133. Devroe E, Engelman A, Silver PA. Intracellular transport of human immunodeficiency virus type 1 integrase. J Cell Sci. 2003 Nov 01; 116(Pt 21):4401-8. PMID: 13130095.
    Citations: 26     Fields:    Translation:HumansCells
  134. Lin CW, Engelman A. The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes. J Virol. 2003 Apr; 77(8):5030-6. PMID: 12663813.
    Citations: 63     Fields:    Translation:HumansAnimalsCells
  135. Engelman A. The roles of cellular factors in retroviral integration. Curr Top Microbiol Immunol. 2003; 281:209-38. PMID: 12932079.
    Citations: 19     Fields:    Translation:HumansAnimalsCells
  136. Limón A, Nakajima N, Lu R, Ghory HZ, Engelman A. Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap. J Virol. 2002 Dec; 76(23):12078-86. PMID: 12414949.
    Citations: 48     Fields:    Translation:HumansCells
  137. Limón A, Devroe E, Lu R, Ghory HZ, Silver PA, Engelman A. Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import. J Virol. 2002 Nov; 76(21):10598-607. PMID: 12368302.
    Citations: 51     Fields:    Translation:HumansCells
  138. Chen H, Engelman A. Asymmetric processing of human immunodeficiency virus type 1 cDNA in vivo: implications for functional end coupling during the chemical steps of DNA transposition. Mol Cell Biol. 2001 Oct; 21(20):6758-67. PMID: 11564861.
    Citations: 33     Fields:    Translation:Cells
  139. Nakajima N, Lu R, Engelman A. Human immunodeficiency virus type 1 replication in the absence of integrase-mediated dna recombination: definition of permissive and nonpermissive T-cell lines. J Virol. 2001 Sep; 75(17):7944-55. PMID: 11483739.
    Citations: 51     Fields:    Translation:HumansCells
  140. Harris D, Engelman A. Both the structure and DNA binding function of the barrier-to-autointegration factor contribute to reconstitution of HIV type 1 integration in vitro. J Biol Chem. 2000 Dec 15; 275(50):39671-7. PMID: 11005805.
    Citations: 23     Fields:    Translation:HumansAnimalsCells
  141. Chen H, Engelman A. Characterization of a replication-defective human immunodeficiency virus type 1 att site mutant that is blocked after the 3' processing step of retroviral integration. J Virol. 2000 Sep; 74(17):8188-93. PMID: 10933731.
    Citations: 9     Fields:    Translation:HumansCells
  142. Brown HE, Chen H, Engelman A. Structure-based mutagenesis of the human immunodeficiency virus type 1 DNA attachment site: effects on integration and cDNA synthesis. J Virol. 1999 Nov; 73(11):9011-20. PMID: 10516007.
    Citations: 52     Fields:    Translation:HumansCells
  143. Chen H, Wei SQ, Engelman A. Multiple integrase functions are required to form the native structure of the human immunodeficiency virus type I intasome. J Biol Chem. 1999 Jun 11; 274(24):17358-64. PMID: 10358097.
    Citations: 56     Fields:    Translation:Cells
  144. Engelman A. In vivo analysis of retroviral integrase structure and function. Adv Virus Res. 1999; 52:411-26. PMID: 10384245.
    Citations: 73     Fields:    Translation:HumansAnimalsCells
  145. Chen H, Engelman A. The barrier-to-autointegration protein is a host factor for HIV type 1 integration. Proc Natl Acad Sci U S A. 1998 Dec 22; 95(26):15270-4. PMID: 9860958.
    Citations: 67     Fields:    Translation:HumansAnimalsCells
  146. Jenkins TM, Esposito D, Engelman A, Craigie R. Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photo-crosslinking. EMBO J. 1997 Nov 17; 16(22):6849-59. PMID: 9362498.
    Citations: 78     Fields:    Translation:Cells
  147. Du Z, Ilyinskii PO, Lally K, Desrosiers RC, Engelman A. A mutation in integrase can compensate for mutations in the simian immunodeficiency virus att site. J Virol. 1997 Nov; 71(11):8124-32. PMID: 9343162.
    Citations: 12     Fields:    Translation:Cells
  148. Engelman A, Liu Y, Chen H, Farzan M, Dyda F. Structure-based mutagenesis of the catalytic domain of human immunodeficiency virus type 1 integrase. J Virol. 1997 May; 71(5):3507-14. PMID: 9094622.
    Citations: 30     Fields:    Translation:HumansCells
  149. Taddeo B, Carlini F, Verani P, Engelman A. Reversion of a human immunodeficiency virus type 1 integrase mutant at a second site restores enzyme function and virus infectivity. J Virol. 1996 Dec; 70(12):8277-84. PMID: 8970947.
    Citations: 11     Fields:    Translation:HumansAnimalsCells
  150. Engelman A. Biochemical characterization of recombinant equine infectious anemia virus integrase. Protein Expr Purif. 1996 Nov; 8(3):299-304. PMID: 8936591.
    Citations: 3     Fields:    Translation:Cells
  151. Jenkins TM, Engelman A, Ghirlando R, Craigie R. A soluble active mutant of HIV-1 integrase: involvement of both the core and carboxyl-terminal domains in multimerization. J Biol Chem. 1996 Mar 29; 271(13):7712-8. PMID: 8631811.
    Citations: 99     Fields:    Translation:HumansCells
  152. Engelman A, Craigie R. Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity. J Virol. 1995 Sep; 69(9):5908-11. PMID: 7637039.
    Citations: 26     Fields:    Translation:Cells
  153. Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM. Solution structure of the DNA binding domain of HIV-1 integrase. Biochemistry. 1995 Aug 08; 34(31):9826-33. PMID: 7632683.
    Citations: 96     Fields:    Translation:Cells
  154. Engelman A, Englund G, Orenstein JM, Martin MA, Craigie R. Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication. J Virol. 1995 May; 69(5):2729-36. PMID: 7535863.
    Citations: 188     Fields:    Translation:HumansCells
  155. Englund G, Theodore TS, Freed EO, Engelman A, Martin MA. Integration is required for productive infection of monocyte-derived macrophages by human immunodeficiency virus type 1. J Virol. 1995 May; 69(5):3216-9. PMID: 7707554.
    Citations: 70     Fields:    Translation:HumansCells
  156. Dyda F, Hickman AB, Jenkins TM, Engelman A, Craigie R, Davies DR. Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. Science. 1994 Dec 23; 266(5193):1981-6. PMID: 7801124.
    Citations: 236     Fields:    Translation:Cells
  157. Vicenzi E, Dimitrov DS, Engelman A, Migone TS, Purcell DF, Leonard J, Englund G, Martin MA. An integration-defective U5 deletion mutant of human immunodeficiency virus type 1 reverts by eliminating additional long terminal repeat sequences. J Virol. 1994 Dec; 68(12):7879-90. PMID: 7966578.
    Citations: 38     Fields:    Translation:HumansCells
  158. Hickman AB, Palmer I, Engelman A, Craigie R, Wingfield P. Biophysical and enzymatic properties of the catalytic domain of HIV-1 integrase. J Biol Chem. 1994 Nov 18; 269(46):29279-87. PMID: 7961898.
    Citations: 22     Fields:    Translation:Cells
  159. Engelman A. Most of the avian genome appears available for retroviral DNA integration. Bioessays. 1994 Nov; 16(11):797-9. PMID: 7840756.
    Citations: 2     Fields:    Translation:AnimalsCells
  160. Engelman A, Hickman AB, Craigie R. The core and carboxyl-terminal domains of the integrase protein of human immunodeficiency virus type 1 each contribute to nonspecific DNA binding. J Virol. 1994 Sep; 68(9):5911-7. PMID: 8057470.
    Citations: 89     Fields:    Translation:Cells
  161. Mazumder A, Engelman A, Craigie R, Fesen M, Pommier Y. Intermolecular disintegration and intramolecular strand transfer activities of wild-type and mutant HIV-1 integrase. Nucleic Acids Res. 1994 Mar 25; 22(6):1037-43. PMID: 8152908.
    Citations: 23     Fields:    Translation:Cells
  162. Engelman A, Bushman FD, Craigie R. Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. EMBO J. 1993 Aug; 12(8):3269-75. PMID: 8344264.
    Citations: 146     Fields:    Translation:Cells
  163. Bushman FD, Engelman A, Palmer I, Wingfield P, Craigie R. Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. Proc Natl Acad Sci U S A. 1993 Apr 15; 90(8):3428-32. PMID: 8386373.
    Citations: 146     Fields:    Translation:Cells
  164. Engelman A, Craigie R. Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J Virol. 1992 Nov; 66(11):6361-9. PMID: 1404595.
    Citations: 200     Fields:    Translation:Cells
  165. Engelman A, Mizuuchi K, Craigie R. HIV-1 DNA integration: mechanism of viral DNA cleavage and DNA strand transfer. Cell. 1991 Dec 20; 67(6):1211-21. PMID: 1760846.
    Citations: 223     Fields:    Translation:Cells
  166. Craigie R, Mizuuchi K, Bushman FD, Engelman A. A rapid in vitro assay for HIV DNA integration. Nucleic Acids Res. 1991 May 25; 19(10):2729-34. PMID: 2041748.
    Citations: 34     Fields:    Translation:AnimalsCells
  167. Engelman A, Rosenberg N. Temperature-sensitive mutants of Abelson murine leukemia virus deficient in protein tyrosine kinase activity. J Virol. 1990 Sep; 64(9):4242-51. PMID: 1696637.
    Citations: 15     Fields:    Translation:AnimalsCells
  168. Engelman A, Rosenberg N. bcr/abl and src but not myc and ras replace v-abl in lymphoid transformation. Mol Cell Biol. 1990 Aug; 10(8):4365-9. PMID: 2164639.
    Citations: 12     Fields:    Translation:AnimalsCells
  169. Engelman A, Rosenberg N. The Abelson protein is required for initiation and maintenance for transformation in murine pre-B cells. Curr Top Microbiol Immunol. 1988; 141:310-5. PMID: 3265094.
    Citations:    Fields:    Translation:AnimalsCells
  170. Engelman A, Rosenberg N. Isolation of temperature-sensitive Abelson virus mutants by site-directed mutagenesis. Proc Natl Acad Sci U S A. 1987 Nov; 84(22):8021-5. PMID: 2825174.
    Citations: 16     Fields:    Translation:AnimalsCells
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