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Sidharth Venkata Puram, M.D.,Ph.D.

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Overview
My scientific background includes training in biochemistry, neurobiology, drug delivery, cancer biology, and residency training in otolaryngology (head and neck surgery). My research began as an undergraduate in the laboratory of Robert Langer at MIT, where I identified novel methods for DNA vaccine delivery using pH-sensitive, biocompatible polymer microspheres (Choleris et al, PNAS 2007; Little et al, Journal of Controlled Release 2005; Little et al, PNAS 2004). Following my undergraduate degrees in Biology and Neuroscience, I entered the Medical Scientist Training Program at Harvard Medical School where I graduated Magna Cum Laude (M.D.) with a PhD in Pathology from the Biology and Biomedical Sciences program. Working in the laboratory of Azad Bonni, I was able to define mechanisms that drive dendrite growth and retraction, including a centrosomal signaling pathway linking calcium signaling at the membrane to the ubiquitin proteasome system (Kim et al., Cell 2009; Puram et al, Nature Neuroscience 2011; Puram et al, Genes and Development, 2011; Puram et al., Cell Reports 2013). These studies identified an entirely novel function for the abundant protein CaMKIIß, which represents 1-2% of protein in the brain, together establishing a major area of research into the homeostatic mechanisms orchestrating dendrite pruning and remodeling (Valnegri*, Puram* et al, Trends in Neuroscience 2015; Puram et al, Development 2015; Puram and Bonni, Seminars in Cell and Developmental Biology 2011; Puram et al, Cell Cycle 2010). I was able to apply skills in biochemistry and cell biology to additional graduate studies on the context-dependent, dual role of the transcription factor STAT3 as an oncogene and tumor suppressor in human glioma models (de la Igelesia et al, Genes and Development 2008; Puram et al, Journal of Neuroscience 2012; Jahani-Asl et al, Nature Neuroscience 2016). These studies challenged the traditional view of transcription factors as unidirectional drivers in oncology, while identifying genetic context-specific pathways that may regulate glioma tumorigenesis (de la Iglesia et al, Current Molecular Medicine 2009). Together, these experiences stimulated a deep interest in tumor biology and the mechanisms that drive growth, invasion, and metastasis, while providing a rich skill set to complete further studies. I am now applying this experience to a post-doctoral project in Brad Bernstein’s laboratory at Massachusetts General Hospital where we are leveraging single cell RNA sequencing to define intratumoral heterogeneity in head and neck squamous cell carcinoma. I have successfully mentored trainees and developed collaborative efforts related to this project, while pursuing additional studies ranging from genomic analyses of adenoid cystic carcinoma, esthesioneuroblastoma, and cutaneous squamous cell carcinoma to basic biological studies of squamous cell differentiation. We hope that our efforts will provide an advanced understanding of diverse cellular populations and the genetic mechanisms governing cancers of the head and neck (Puram et al, Hematology Oncology Clinics of North America 2015). My clinical interests include electrophysiologic parameters defining intraoperative injury to the recurrent laryngeal nerve, transfusion outcomes following head and neck free and pedicled flap reconstruction, and national health care metrics after head and neck cancer surgery.

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Abt NB, Tarabanis C, Miller AL, Puram SV, Varvares MA. Preoperative anemia displays a dose-dependent effect on complications in head and neck oncologic surgery. Head Neck. 2019 Apr 29. PMID: 31034668.
    Citations:    
  2. Barbarite E, Puram SV, Derakhshan A, Rossi ED, Faquin WC, Varvares MA. A Call for Universal Acceptance of the Milan System for Reporting Salivary Gland Cytopathology. Laryngoscope. 2019 Mar 08. PMID: 30848480.
    Citations:    
  3. Puram SV, Bhattacharyya N. Surgery versus radiation for T1 glottic carcinoma: Second primary considerations. Laryngoscope. 2019 Feb 15. PMID: 30767222.
    Citations:    
  4. Silverman DA, Puram SV, Rocco JW, Old MO, Kang SY. Salvage laryngectomy following organ-preservation therapy - An evidence-based review. Oral Oncol. 2019 Jan; 88:137-144. PMID: 30616784.
    Citations:    Fields:    
  5. Yu PK, Sethi RKV, Rathi V, Puram SV, Lin DT, Emerick KS, Durand ML, Deschler DG. Postoperative care in an intermediate-level medical unit after head and neck microvascular free flap reconstruction. Laryngoscope Investig Otolaryngol. 2019 Feb; 4(1):39-42. PMID: 30828617.
    Citations:    
  6. Holbrook EH, Puram SV, See RB, Tripp AG, Nair DG. Induction of smell through transethmoid electrical stimulation of the olfactory bulb. Int Forum Allergy Rhinol. 2019 Feb; 9(2):158-164. PMID: 30480384.
    Citations:    Fields:    
  7. Abt NB, Puram SV, Sinha S, Sethi RKV, Goyal N, Emerick KS, Lin DT, Deschler DG. Transfusion in Head and Neck Cancer Patients Undergoing Pedicled Flap Reconstruction. Laryngoscope. 2018 12; 128(12):E409-E415. PMID: 30247764.
    Citations:    Fields:    
  8. Sethi RKV, Panth N, Puram SV, Varvares MA. Opioid Prescription Patterns Among Patients With Head and Neck Cancer. JAMA Otolaryngol Head Neck Surg. 2018 Apr 01; 144(4):382-383. PMID: 29522065.
    Citations:    Fields:    
  9. Puram SV, Parikh AS, Tirosh I. Single cell RNA-seq highlights a role for a partial EMT in head and neck cancer. Mol Cell Oncol. 2018; 5(3):e1448244. PMID: 30250901.
    Citations:    
  10. Abt NB, Sethi RK, Puram SV, Varvares MA. Preoperative laboratory data are associated with complications and surgical site infection in composite head and neck surgical resections. Am J Otolaryngol. 2018 May - Jun; 39(3):261-265. PMID: 29398185.
    Citations:    Fields:    Translation:Humans
  11. Puram SV, Bhattacharyya N. Identifying Metrics before and after Readmission following Head and Neck Surgery and Factors Affecting Readmission Rate. Otolaryngol Head Neck Surg. 2018 May; 158(5):860-866. PMID: 29336226.
    Citations:    Fields:    
  12. Puram SV, Tirosh I, Parikh AS, Patel AP, Yizhak K, Gillespie S, Rodman C, Luo CL, Mroz EA, Emerick KS, Deschler DG, Varvares MA, Mylvaganam R, Rozenblatt-Rosen O, Rocco JW, Faquin WC, Lin DT, Regev A, Bernstein BE. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell. 2017 Dec 14; 171(7):1611-1624.e24. PMID: 29198524.
    Citations: 19     Fields:    Translation:HumansCells
  13. Barber SR, Kozin ED, Remenschneider AK, Puram SV, Smith M, Herrmann BS, Cunnane ME, Brown MC, Lee DJ. Auditory Brainstem Implant Array Position Varies Widely Among Adult and Pediatric Patients and Is Associated With Perception. Ear Hear. 2017 Nov/Dec; 38(6):e343-e351. PMID: 28700445.
    Citations:    Fields:    Translation:Humans
  14. Rathi VK, Gadkaree SK, Ross JS, Kozin ED, Sethi RK, Naunheim MR, Puram SV, Gray ST. US Food and Drug Administration Clearance of Moderate-Risk Otolaryngologic Devices via the 510(k) Process, 1997-2016. Otolaryngol Head Neck Surg. 2017 10; 157(4):608-617. PMID: 28786317.
    Citations:    Fields:    Translation:HumansPHPublic Health
  15. Abt NB, Xie Y, Puram SV, Richmon JD, Varvares MA. Frailty index: Intensive care unit complications in head and neck oncologic regional and free flap reconstruction. Head Neck. 2017 08; 39(8):1578-1585. PMID: 28449296.
    Citations: 2     Fields:    Translation:Humans
  16. Puram SV, Doody J, Cunningham MJ. A Young Boy With Progressive Dysphonia. JAMA Otolaryngol Head Neck Surg. 2017 03 01; 143(3):313-314. PMID: 28097294.
    Citations:    Fields:    Translation:Humans
  17. Abt NB, Srikanth P, Puram SV, Deschler DG. Repair of complex pharyngocutaneous fistula using a staged temporoparietal fascial flap. Am J Otolaryngol. 2017 Mar - Apr; 38(2):254-256. PMID: 27916282.
    Citations:    Fields:    Translation:Humans
  18. Sinha S, Puram SV, Sethi RK, Goyal N, Emerick KS, Lin D, Durand ML, Deschler DG. Perioperative Deep Vein Thrombosis Risk Stratification. Otolaryngol Head Neck Surg. 2017 01; 156(1):118-121. PMID: 27600631.
    Citations:    Fields:    Translation:Humans
  19. Puram SV, Bhattacharyya N. Quality Indicators for Head and Neck Oncologic Surgery: Academic versus Nonacademic Outcomes. Otolaryngol Head Neck Surg. 2016 11; 155(5):733-739. PMID: 27329425.
    Citations: 1     Fields:    Translation:Humans
  20. Puram SV, Chow H, Wu CW, Heaton JT, Kamani D, Gorti G, Chiang FY, Dionigi G, Barczynski M, Schneider R, Dralle H, Lorenz K, Randolph GW. Posterior cricoarytenoid muscle electrophysiologic changes are predictive of vocal cord paralysis with recurrent laryngeal nerve compressive injury in a canine model. Laryngoscope. 2016 12; 126(12):2744-2751. PMID: 27113438.
    Citations: 1     Fields:    Translation:Animals
  21. Jahani-Asl A, Yin H, Soleimani VD, Haque T, Luchman HA, Chang NC, Sincennes MC, Puram SV, Scott AM, Lorimer IA, Perkins TJ, Ligon KL, Weiss S, Rudnicki MA, Bonni A. Control of glioblastoma tumorigenesis by feed-forward cytokine signaling. Nat Neurosci. 2016 06; 19(6):798-806. PMID: 27110918.
    Citations: 15     Fields:    Translation:HumansAnimalsCells
  22. Puram SV, Barber SR, Kozin ED, Shah P, Remenschneider A, Herrmann BS, Duhaime AC, Barker FG, Lee DJ. Outcomes following Pediatric Auditory Brainstem Implant Surgery: Early Experiences in a North American Center. Otolaryngol Head Neck Surg. 2016 07; 155(1):133-8. PMID: 27095049.
    Citations: 5     Fields:    Translation:Humans
  23. Puram SV, Lee DJ. Pediatric Auditory Brainstem Implant Surgery. Otolaryngol Clin North Am. 2015 Dec; 48(6):1117-48. PMID: 26553310.
    Citations: 1     Fields:    Translation:Humans
  24. Puram SV, Rocco JW. Molecular Aspects of Head and Neck Cancer Therapy. Hematol Oncol Clin North Am. 2015 Dec; 29(6):971-92. PMID: 26568543.
    Citations: 5     Fields:    Translation:HumansAnimalsCells
  25. Puram SV, Chow H, Wu CW, Heaton JT, Kamani D, Gorti G, Chiang FY, Dionigi G, Barczynski M, Schneider R, Dralle H, Lorenz K, Randolph GW. Vocal cord paralysis predicted by neural monitoring electrophysiologic changes with recurrent laryngeal nerve compressive neuropraxic injury in a canine model. Head Neck. 2016 04; 38 Suppl 1:E1341-50. PMID: 26348472.
    Citations: 6     Fields:    Translation:Animals
  26. Valnegri P, Puram SV, Bonni A. Regulation of dendrite morphogenesis by extrinsic cues. Trends Neurosci. 2015 Jul; 38(7):439-47. PMID: 26100142.
    Citations: 15     Fields:    Translation:HumansAnimalsCells
  27. Puram SV, Herrmann B, Barker FG, Lee DJ. Retrosigmoid Craniotomy for Auditory Brainstem Implantation in Adult Patients with Neurofibromatosis Type 2. J Neurol Surg B Skull Base. 2015 Dec; 76(6):440-50. PMID: 27054058.
    Citations:    
  28. Puram SV, Carter MS, Deschler D. Maggot infestation of an ulcerated neck wound. Ear Nose Throat J. 2015 Jun; 94(6):E47-8. PMID: 26053993.
    Citations:    Fields:    Translation:Humans
  29. Mohan S, Puram SV, Yarlagadda B, Nosé V, Deschler DG. Ossifying Parotid Carcinoma ex Pleomorphic Adenoma. Case Rep Otolaryngol. 2015; 2015:395358. PMID: 26075129.
    Citations: 1     
  30. Puram SV, Tward AD, Jung DH, Dilger AE, Herrmann BS, Duhaime AC, Barker FG, Lee DJ. Auditory brainstem implantation in a 16-month-old boy with cochlear hypoplasia. Otol Neurotol. 2015 Apr; 36(4):618-24. PMID: 25473959.
    Citations: 2     Fields:    Translation:HumansCTClinical Trials
  31. Noij KS, Remenschneider AK, Kozin ED, Puram S, Herrmann B, Cohen M, Cunnane MB, Lee DJ. Direct parasagittal magnetic resonance imaging of the internal auditory canal to determine cochlear or auditory brainstem implant candidacy in children. Laryngoscope. 2015 Oct; 125(10):2382-5. PMID: 25778542.
    Citations:    Fields:    Translation:Humans
  32. Puram SV, Yarlagadda BB, Sethi R, Muralidhar V, Chambers KJ, Emerick KS, Rocco JW, Lin DT, Deschler DG. Transfusion in head and neck free flap patients: practice patterns and a comparative analysis by flap type. Otolaryngol Head Neck Surg. 2015 Mar; 152(3):449-57. PMID: 25628368.
    Citations: 1     Fields:    Translation:Humans
  33. Kaplan AB, Kozin ED, Puram SV, Owoc MS, Shah PV, Hight AE, Sethi RKV, Remenschneider AK, Lee DJ. Auditory brainstem implant candidacy in the United States in children 0-17 years old. Int J Pediatr Otorhinolaryngol. 2015 Mar; 79(3):310-315. PMID: 25577282.
    Citations: 3     Fields:    Translation:Humans
  34. Puram SV, Kozin ED, Sethi RK, Hight AE, Shrime MG, Gray ST, Cohen MS, Lee DJ. Influence of trainee participation on operative times for adult and pediatric cochlear implantation. Cochlear Implants Int. 2015 May; 16(3):175-9. PMID: 25387322.
    Citations:    Fields:    Translation:Humans
  35. Puram SV, Kozin ED, Sethi R, Alkire B, Lee DJ, Gray ST, Shrime MG, Cohen M. Impact of resident surgeons on procedure length based on common pediatric otolaryngology cases. Laryngoscope. 2015 Apr; 125(4):991-7. PMID: 25251257.
    Citations: 4     Fields:    Translation:Humans
  36. Puram SV, Bonni A. Cell-intrinsic drivers of dendrite morphogenesis. Development. 2013 Dec; 140(23):4657-71. PMID: 24255095.
    Citations: 33     Fields:    Translation:AnimalsCells
  37. Puram SV, Roberts DS, Niesten ME, Dilger AE, Lee DJ. Cochlear implant outcomes in patients with superior canal dehiscence. Cochlear Implants Int. 2015 Jul; 16(4):213-21. PMID: 24074366.
    Citations: 1     Fields:    Translation:Humans
  38. Puram SV, Kim AH, Park HY, Anckar J, Bonni A. The ubiquitin receptor S5a/Rpn10 links centrosomal proteasomes with dendrite development in the mammalian brain. Cell Rep. 2013 Jul 11; 4(1):19-30. PMID: 23831032.
    Citations: 16     Fields:    Translation:AnimalsCells
  39. Puram SV, Hasserjian RP, Faquin WC, Lin HW, Rocco JW. Castleman disease presenting in the neck: report of a case and review of the literature. Am J Otolaryngol. 2013 May-Jun; 34(3):239-44. PMID: 23375540.
    Citations: 3     Fields:    Translation:Humans
  40. Puram SV, Yeung CM, Jahani-Asl A, Lin C, de la Iglesia N, Konopka G, Jackson-Grusby L, Bonni A. STAT3-iNOS Signaling Mediates EGFRvIII-Induced Glial Proliferation and Transformation. J Neurosci. 2012 Jun 06; 32(23):7806-18. PMID: 22674257.
    Citations: 13     Fields:    Translation:AnimalsCells
  41. Puram SV, Riccio A, Koirala S, Ikeuchi Y, Kim AH, Corfas G, Bonni A. A TRPC5-regulated calcium signaling pathway controls dendrite patterning in the mammalian brain. Genes Dev. 2011 Dec 15; 25(24):2659-73. PMID: 22135323.
    Citations: 17     Fields:    Translation:AnimalsCells
  42. Puram SV, Kim AH, Ikeuchi Y, Wilson-Grady JT, Merdes A, Gygi SP, Bonni A. A CaMKIIß signaling pathway at the centrosome regulates dendrite patterning in the brain. Nat Neurosci. 2011 Jul 03; 14(8):973-83. PMID: 21725312.
    Citations: 35     Fields:    Translation:AnimalsCells
  43. Puram SV, Bonni A. Novel functions for the anaphase-promoting complex in neurobiology. Semin Cell Dev Biol. 2011 Aug; 22(6):586-94. PMID: 21439392.
    Citations: 17     Fields:    Translation:HumansAnimalsCells
  44. Puram SV, Kim AH, Bonni A. An old dog learns new tricks: a novel function for Cdc20-APC in dendrite morphogenesis in neurons. Cell Cycle. 2010 Feb 01; 9(3):482-5. PMID: 20195072.
    Citations: 6     Fields:    Translation:AnimalsCells
  45. de la Iglesia N, Puram SV, Bonni A. STAT3 regulation of glioblastoma pathogenesis. Curr Mol Med. 2009 Jun; 9(5):580-90. PMID: 19601808.
    Citations: 36     Fields:    Translation:HumansAnimals
  46. Kim AH, Puram SV, Bilimoria PM, Ikeuchi Y, Keough S, Wong M, Rowitch D, Bonni A. A centrosomal Cdc20-APC pathway controls dendrite morphogenesis in postmitotic neurons. Cell. 2009 Jan 23; 136(2):322-36. PMID: 19167333.
    Citations: 95     Fields:    Translation:AnimalsCells
  47. de la Iglesia N, Konopka G, Puram SV, Chan JA, Bachoo RM, You MJ, Levy DE, Depinho RA, Bonni A. Identification of a PTEN-regulated STAT3 brain tumor suppressor pathway. Genes Dev. 2008 Feb 15; 22(4):449-62. PMID: 18258752.
    Citations: 135     Fields:    Translation:HumansAnimalsCells
  48. Choleris E, Little SR, Mong JA, Puram SV, Langer R, Pfaff DW. Microparticle-based delivery of oxytocin receptor antisense DNA in the medial amygdala blocks social recognition in female mice. Proc Natl Acad Sci U S A. 2007 Mar 13; 104(11):4670-5. PMID: 17360582.
    Citations: 31     Fields:    Translation:Animals
  49. Little SR, Lynn DM, Puram SV, Langer R. Formulation and characterization of poly (beta amino ester) microparticles for genetic vaccine delivery. J Control Release. 2005 Oct 20; 107(3):449-62. PMID: 16112767.
    Citations: 19     Fields:    Translation:AnimalsCells
  50. Little SR, Lynn DM, Ge Q, Anderson DG, Puram SV, Chen J, Eisen HN, Langer R. Poly-beta amino ester-containing microparticles enhance the activity of nonviral genetic vaccines. Proc Natl Acad Sci U S A. 2004 Jun 29; 101(26):9534-9. PMID: 15210954.
    Citations: 54     Fields:    Translation:AnimalsCells
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Funded by the NIH/NCATS Clinical and Translational Science Award (CTSA) program, grant number UL1TR001102, and through institutional support from Harvard University, Harvard Medical School, Harvard T.H. Chan School of Public Health, Beth Israel Deaconess Medical Center, Boston Children's Hospital, Brigham and Women's Hospital, Massachusetts General Hospital and the Dana Farber Cancer Institute.