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Last Name

profileSidharth Venkata Puram, M.D.,Ph.D.

TitleClinical Fellow in Otolaryngology (EXT)
InstitutionMassachusetts Eye and Ear Infirmary
AddressMassachusetts Eye & Ear Infrm
Otology and Laryngology
243 Charles St
Boston MA 02114
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Other Positions
TitleResearch Assistant Graduate Student in Neurobiology
InstitutionHarvard Medical School

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My scientific background includes training in biochemistry, neurobiology, drug delivery, cancer biology, and residency training in otolaryngology (head and neck surgery). My research began as an undergraduate in the laboratory of Robert Langer at MIT, where I identified novel methods for DNA vaccine delivery using pH-sensitive, biocompatible polymer microspheres (Choleris et al, PNAS 2007; Little et al, Journal of Controlled Release 2005; Little et al, PNAS 2004). Following my undergraduate degrees in Biology and Neuroscience, I entered the Medical Scientist Training Program at Harvard Medical School where I graduated Magna Cum Laude (M.D.) with a PhD in Pathology from the Biology and Biomedical Sciences program. Working in the laboratory of Azad Bonni, I was able to define mechanisms that drive dendrite growth and retraction, including a centrosomal signaling pathway linking calcium signaling at the membrane to the ubiquitin proteasome system (Kim et al., Cell 2009; Puram et al, Nature Neuroscience 2011; Puram et al, Genes and Development, 2011; Puram et al., Cell Reports 2013). These studies identified an entirely novel function for the abundant protein CaMKIIß, which represents 1-2% of protein in the brain, together establishing a major area of research into the homeostatic mechanisms orchestrating dendrite pruning and remodeling (Valnegri*, Puram* et al, Trends in Neuroscience 2015; Puram et al, Development 2015; Puram and Bonni, Seminars in Cell and Developmental Biology 2011; Puram et al, Cell Cycle 2010). I was able to apply skills in biochemistry and cell biology to additional graduate studies on the context-dependent, dual role of the transcription factor STAT3 as an oncogene and tumor suppressor in human glioma models (de la Igelesia et al, Genes and Development 2008; Puram et al, Journal of Neuroscience 2012; Jahani-Asl et al, Nature Neuroscience 2016). These studies challenged the traditional view of transcription factors as unidirectional drivers in oncology, while identifying genetic context-specific pathways that may regulate glioma tumorigenesis (de la Iglesia et al, Current Molecular Medicine 2009). Together, these experiences stimulated a deep interest in tumor biology and the mechanisms that drive growth, invasion, and metastasis, while providing a rich skill set to complete further studies. I am now applying this experience to a post-doctoral project in Brad Bernstein’s laboratory at Massachusetts General Hospital where we are leveraging single cell RNA sequencing to define intratumoral heterogeneity in head and neck squamous cell carcinoma. I have successfully mentored trainees and developed collaborative efforts related to this project, while pursuing additional studies ranging from genomic analyses of adenoid cystic carcinoma, esthesioneuroblastoma, and cutaneous squamous cell carcinoma to basic biological studies of squamous cell differentiation. We hope that our efforts will provide an advanced understanding of diverse cellular populations and the genetic mechanisms governing cancers of the head and neck (Puram et al, Hematology Oncology Clinics of North America 2015). My clinical interests include electrophysiologic parameters defining intraoperative injury to the recurrent laryngeal nerve, transfusion outcomes following head and neck free and pedicled flap reconstruction, and national health care metrics after head and neck cancer surgery.

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Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Abt NB, Sethi RK, Puram SV, Varvares MA. Preoperative laboratory data are associated with complications and surgical site infection in composite head and neck surgical resections. Am J Otolaryngol. 2018 Jan 31. PMID: 29398185.
    View in: PubMed
  2. Puram SV, Bhattacharyya N. Identifying Metrics before and after Readmission following Head and Neck Surgery and Factors Affecting Readmission Rate. Otolaryngol Head Neck Surg. 2018 Jan 01; 194599817750373. PMID: 29336226.
    View in: PubMed
  3. Puram SV, Tirosh I, Parikh AS, Patel AP, Yizhak K, Gillespie S, Rodman C, Luo CL, Mroz EA, Emerick KS, Deschler DG, Varvares MA, Mylvaganam R, Rozenblatt-Rosen O, Rocco JW, Faquin WC, Lin DT, Regev A, Bernstein BE. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer. Cell. 2017 Dec 14; 171(7):1611-1624.e24. PMID: 29198524.
    View in: PubMed
  4. Barber SR, Kozin ED, Remenschneider AK, Puram SV, Smith M, Herrmann BS, Cunnane ME, Brown MC, Lee DJ. Auditory Brainstem Implant Array Position Varies Widely Among Adult and Pediatric Patients and Is Associated With Perception. Ear Hear. 2017 Nov/Dec; 38(6):e343-e351. PMID: 28700445.
    View in: PubMed
  5. Rathi VK, Gadkaree SK, Ross JS, Kozin ED, Sethi RK, Naunheim MR, Puram SV, Gray ST. US Food and Drug Administration Clearance of Moderate-Risk Otolaryngologic Devices via the 510(k) Process, 1997-2016. Otolaryngol Head Neck Surg. 2017 Oct; 157(4):608-617. PMID: 28786317.
    View in: PubMed
  6. Abt NB, Xie Y, Puram SV, Richmon JD, Varvares MA. Frailty index: Intensive care unit complications in head and neck oncologic regional and free flap reconstruction. Head Neck. 2017 Aug; 39(8):1578-1585. PMID: 28449296.
    View in: PubMed
  7. Puram SV, Doody J, Cunningham MJ. A Young Boy With Progressive Dysphonia. JAMA Otolaryngol Head Neck Surg. 2017 Mar 01; 143(3):313-314. PMID: 28097294.
    View in: PubMed
  8. Abt NB, Srikanth P, Puram SV, Deschler DG. Repair of complex pharyngocutaneous fistula using a staged temporoparietal fascial flap. Am J Otolaryngol. 2017 Mar - Apr; 38(2):254-256. PMID: 27916282.
    View in: PubMed
  9. Sinha S, Puram SV, Sethi RK, Goyal N, Emerick KS, Lin D, Durand ML, Deschler DG. Perioperative Deep Vein Thrombosis Risk Stratification. Otolaryngol Head Neck Surg. 2017 Jan; 156(1):118-121. PMID: 27600631.
    View in: PubMed
  10. Puram SV, Bhattacharyya N. Quality Indicators for Head and Neck Oncologic Surgery: Academic versus Nonacademic Outcomes. Otolaryngol Head Neck Surg. 2016 Nov; 155(5):733-739. PMID: 27329425.
    View in: PubMed
  11. Puram SV, Chow H, Wu CW, Heaton JT, Kamani D, Gorti G, Chiang FY, Dionigi G, Barczynski M, Schneider R, Dralle H, Lorenz K, Randolph GW. Posterior cricoarytenoid muscle electrophysiologic changes are predictive of vocal cord paralysis with recurrent laryngeal nerve compressive injury in a canine model. Laryngoscope. 2016 Dec; 126(12):2744-2751. PMID: 27113438.
    View in: PubMed
  12. Jahani-Asl A, Yin H, Soleimani VD, Haque T, Luchman HA, Chang NC, Sincennes MC, Puram SV, Scott AM, Lorimer IA, Perkins TJ, Ligon KL, Weiss S, Rudnicki MA, Bonni A. Control of glioblastoma tumorigenesis by feed-forward cytokine signaling. Nat Neurosci. 2016 06; 19(6):798-806. PMID: 27110918; PMCID: PMC4984527.
  13. Puram SV, Barber SR, Kozin ED, Shah P, Remenschneider A, Herrmann BS, Duhaime AC, Barker FG, Lee DJ. Outcomes following Pediatric Auditory Brainstem Implant Surgery: Early Experiences in a North American Center. Otolaryngol Head Neck Surg. 2016 07; 155(1):133-8. PMID: 27095049.
    View in: PubMed
  14. Puram SV, Lee DJ. Pediatric Auditory Brainstem Implant Surgery. Otolaryngol Clin North Am. 2015 Dec; 48(6):1117-48. PMID: 26553310.
    View in: PubMed
  15. Puram SV, Rocco JW. Molecular Aspects of Head and Neck Cancer Therapy. Hematol Oncol Clin North Am. 2015 Dec; 29(6):971-92. PMID: 26568543; PMCID: PMC4648693 [Available on 12/01/16].
  16. Puram SV, Chow H, Wu CW, Heaton JT, Kamani D, Gorti G, Chiang FY, Dionigi G, Barczynski M, Schneider R, Dralle H, Lorenz K, Randolph GW. Vocal cord paralysis predicted by neural monitoring electrophysiologic changes with recurrent laryngeal nerve compressive neuropraxic injury in a canine model. Head Neck. 2016 Apr; 38 Suppl 1:E1341-50. PMID: 26348472.
    View in: PubMed
  17. Valnegri P, Puram SV, Bonni A. Regulation of dendrite morphogenesis by extrinsic cues. Trends Neurosci. 2015 Jul; 38(7):439-47. PMID: 26100142; PMCID: PMC4492469.
  18. Puram SV, Herrmann B, Barker FG, Lee DJ. Retrosigmoid Craniotomy for Auditory Brainstem Implantation in Adult Patients with Neurofibromatosis Type 2. J Neurol Surg B Skull Base. 2015 Dec; 76(6):440-50. PMID: 27054058; PMCID: PMC4813683 [Available on 12/01/16].
  19. Puram SV, Carter MS, Deschler D. Maggot infestation of an ulcerated neck wound. Ear Nose Throat J. 2015 Jun; 94(6):E47-8. PMID: 26053993.
    View in: PubMed
  20. Mohan S, Puram SV, Yarlagadda B, Nosé V, Deschler DG. Ossifying Parotid Carcinoma ex Pleomorphic Adenoma. Case Rep Otolaryngol. 2015; 2015:395358. PMID: 26075129; PMCID: PMC4449911.
  21. Puram SV, Tward AD, Jung DH, Dilger AE, Herrmann BS, Duhaime AC, Barker FG, Lee DJ. Auditory brainstem implantation in a 16-month-old boy with cochlear hypoplasia. Otol Neurotol. 2015 Apr; 36(4):618-24. PMID: 25473959.
    View in: PubMed
  22. Noij KS, Remenschneider AK, Kozin ED, Puram S, Herrmann B, Cohen M, Cunnane MB, Lee DJ. Direct parasagittal magnetic resonance imaging of the internal auditory canal to determine cochlear or auditory brainstem implant candidacy in children. Laryngoscope. 2015 Oct; 125(10):2382-5. PMID: 25778542.
    View in: PubMed
  23. Puram SV, Yarlagadda BB, Sethi R, Muralidhar V, Chambers KJ, Emerick KS, Rocco JW, Lin DT, Deschler DG. Transfusion in head and neck free flap patients: practice patterns and a comparative analysis by flap type. Otolaryngol Head Neck Surg. 2015 Mar; 152(3):449-57. PMID: 25628368.
    View in: PubMed
  24. Kaplan AB, Kozin ED, Puram SV, Owoc MS, Shah PV, Hight AE, Sethi RKV, Remenschneider AK, Lee DJ. Auditory brainstem implant candidacy in the United States in children 0-17 years old. Int J Pediatr Otorhinolaryngol. 2015 Mar; 79(3):310-315. PMID: 25577282; PMCID: PMC4477282.
  25. Puram SV, Kozin ED, Sethi RK, Hight AE, Shrime MG, Gray ST, Cohen MS, Lee DJ. Influence of trainee participation on operative times for adult and pediatric cochlear implantation. Cochlear Implants Int. 2015 May; 16(3):175-9. PMID: 25387322; PMCID: PMC4476316.
  26. Puram SV, Kozin ED, Sethi R, Alkire B, Lee DJ, Gray ST, Shrime MG, Cohen M. Impact of resident surgeons on procedure length based on common pediatric otolaryngology cases. Laryngoscope. 2015 Apr; 125(4):991-7. PMID: 25251257; PMCID: PMC4469133.
  27. Puram SV, Bonni A. Cell-intrinsic drivers of dendrite morphogenesis. Development. 2013 Dec; 140(23):4657-71. PMID: 24255095; PMCID: PMC3833426.
  28. Puram SV, Roberts DS, Niesten ME, Dilger AE, Lee DJ. Cochlear implant outcomes in patients with superior canal dehiscence. Cochlear Implants Int. 2015 Jul; 16(4):213-21. PMID: 24074366.
    View in: PubMed
  29. Puram SV, Kim AH, Park HY, Anckar J, Bonni A. The ubiquitin receptor S5a/Rpn10 links centrosomal proteasomes with dendrite development in the mammalian brain. Cell Rep. 2013 Jul 11; 4(1):19-30. PMID: 23831032; PMCID: PMC4103748.
  30. Puram SV, Hasserjian RP, Faquin WC, Lin HW, Rocco JW. Castleman disease presenting in the neck: report of a case and review of the literature. Am J Otolaryngol. 2013 May-Jun; 34(3):239-44. PMID: 23375540.
    View in: PubMed
  31. Puram SV, Yeung CM, Jahani-Asl A, Lin C, de la Iglesia N, Konopka G, Jackson-Grusby L, Bonni A. STAT3-iNOS Signaling Mediates EGFRvIII-Induced Glial Proliferation and Transformation. J Neurosci. 2012 Jun 06; 32(23):7806-18. PMID: 22674257; PMCID: PMC3409246.
  32. Puram SV, Riccio A, Koirala S, Ikeuchi Y, Kim AH, Corfas G, Bonni A. A TRPC5-regulated calcium signaling pathway controls dendrite patterning in the mammalian brain. Genes Dev. 2011 Dec 15; 25(24):2659-73. PMID: 22135323; PMCID: PMC3248686.
  33. Puram SV, Kim AH, Ikeuchi Y, Wilson-Grady JT, Merdes A, Gygi SP, Bonni A. A CaMKIIß signaling pathway at the centrosome regulates dendrite patterning in the brain. Nat Neurosci. 2011 Jul 03; 14(8):973-83. PMID: 21725312; PMCID: PMC3391735.
  34. Puram SV, Bonni A. Novel functions for the anaphase-promoting complex in neurobiology. Semin Cell Dev Biol. 2011 Aug; 22(6):586-94. PMID: 21439392; PMCID: PMC3177029.
  35. Puram SV, Kim AH, Bonni A. An old dog learns new tricks: a novel function for Cdc20-APC in dendrite morphogenesis in neurons. Cell Cycle. 2010 Feb 01; 9(3):482-5. PMID: 20195072; PMCID: PMC2853878.
  36. de la Iglesia N, Puram SV, Bonni A. STAT3 regulation of glioblastoma pathogenesis. Curr Mol Med. 2009 Jun; 9(5):580-90. PMID: 19601808; PMCID: PMC2712135.
  37. Kim AH, Puram SV, Bilimoria PM, Ikeuchi Y, Keough S, Wong M, Rowitch D, Bonni A. A centrosomal Cdc20-APC pathway controls dendrite morphogenesis in postmitotic neurons. Cell. 2009 Jan 23; 136(2):322-36. PMID: 19167333; PMCID: PMC2707082.
  38. de la Iglesia N, Konopka G, Puram SV, Chan JA, Bachoo RM, You MJ, Levy DE, Depinho RA, Bonni A. Identification of a PTEN-regulated STAT3 brain tumor suppressor pathway. Genes Dev. 2008 Feb 15; 22(4):449-62. PMID: 18258752; PMCID: PMC2238667.
  39. Choleris E, Little SR, Mong JA, Puram SV, Langer R, Pfaff DW. Microparticle-based delivery of oxytocin receptor antisense DNA in the medial amygdala blocks social recognition in female mice. Proc Natl Acad Sci U S A. 2007 Mar 13; 104(11):4670-5. PMID: 17360582; PMCID: PMC1838659.
  40. Little SR, Lynn DM, Puram SV, Langer R. Formulation and characterization of poly (beta amino ester) microparticles for genetic vaccine delivery. J Control Release. 2005 Oct 20; 107(3):449-62. PMID: 16112767.
    View in: PubMed
  41. Little SR, Lynn DM, Ge Q, Anderson DG, Puram SV, Chen J, Eisen HN, Langer R. Poly-beta amino ester-containing microparticles enhance the activity of nonviral genetic vaccines. Proc Natl Acad Sci U S A. 2004 Jun 29; 101(26):9534-9. PMID: 15210954; PMCID: PMC470709.
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