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profileLynn Bry, PH.D., M.D.

TitleAssociate Professor of Pathology
InstitutionBrigham and Women's Hospital
DepartmentPathology
AddressBrigham and Womens Hospital
Dept. Pathology, EBRC, 411
221 Longwood Ave
Boston MA 02115
Phone617/732-7763
Fax617/264-6898
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Research: Mucosal Immunology/Infectious Disease

My laboratory studies the following aspects of host defense against microbial pathogens (1) host reponses against attaching and effacing pathogens such as Citrobacter rodentium and the enteropathogenic E. coli (EPEC), (2) the molecular basis of immunoprotection from colonization with commensals and (3) recruitment of adaptive memory responses against non-invasive enteric pathogens to fight primary infection with phylogenetically related invasive species.

We have defined the effector functions and locations at which protective T cell and B cell responses develop against the murine attaching and effacing pathogen Citrobacter rodentium. This EPEC-like organism produces a primary infection of the apical surface of the gut epithelium. Surprisingly, systemic and CD4+ T cell dependent IgG, and not mucosal IgA, proved the critical adaptive response needed to successfully control mucosal infection. Pathogen-specific IgG acts systemically and in the gut lumen to effect erradication of pathogens from the host. We have subsequently shown that interactions with complement are important for this erradication and for the development of specific IgG.

We have further developed this model to elucidate how the commensal flora primes adaptive responses recruited early in infection with C. rodentium. By forming the HDDC Gnotobiotic Mouse Core, we expect to be able to define how members of the commensal flora provide protection against phylogenetically related or unrelated organisms, through mechanisms including colonization resistance as well as cognate and non-cognate effects upon the immune system.

Research: Crimson Application - IT infrastructure to support high-through sample collection and downstream applications for a research enterprise.

Crimson prospectively matches discarded samples from clinical laboratories per investigator-defined criteria. This feature effectively harnesses the thousands of samples and associated data generated daily in clinical laboratories for productive use in IRB-approved studies. Additional functions of Crimson include management of sample inventories, user workgroups, and auditing capabilities to insure compliance with HIPAA and local IRBs. As of May 2007, Crimson has been integrated with the Research Patient Data Registry (RPDR), a clinical data repository that houses electronic information generated at BWH and MGH. This integration allows an investigator to define a cohort of patients on the RPDR and forward it to Crimson for collection of discarded samples.

Crimson went live on 2/1/2007 and has since handled >15,000 samples in its first year of operations, including >10,000 clinical trials samples and >5,000 discarded pathology samples collected for use by IRB-approved studies. The application was developed by myself and Neil Herring in the Pathology IT Division at BWH with software development support for Daedalus Software Inc. in Cambridge MA. Crimson remains a unique an innovative system, one that is being adopted by i2b2 (Informatics for Integrating Biology to the Bedside), an NIH Roadmap initiative.


Teaching:

I teach in the medical school courses IN704.0: "Immunology, Microbiology and Infectious Disease," HST-040: "Mechanisms of Microbial Pathogenesis" and the clerkship PA511M.J: "Clinical Laboratory Medicine." I am also course director for the clinical microbiology rotation at Brigham & Women's Hospital through which pathology residents and infectious disease fellows rotate, and organize the weekly plate rounds between the Clinical Laboratories and Division of Infectious Disease at Brigham & Women’s Hospital.

I am also a lecturer and mentor for the Project Success Program at Harvard Medical School.

Clinical:

As Associate Medical Director in the Clinical Microbiology Laboratory at Brigham and Women's Hospital, my duties include oversight of routine microbiological testing, evaluation and implementation of new modalities for diagnosing infectious diseases and determining antimicrobial resistance.

Since 2006 I have successfully worked to bring in additional high-throughput platforms for the molecular diagnosis of infectious agents, including HIV, HCV, gonorrhea and Chlamydia. I have also worked with clinicians in the renal division to implement decision/support trees based on key data from testing for BK virus in renal transplant patients, and with individuals in the pulmonary division to assist with microbiological assessment of cystic fibrosis patients seen at BWH.


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eagle-i@hms.harvard.edu.
BWH Specimen Bank - Core services (10) and Protocols (4)
Gnotobiotic and Microbiology Core (BCH) - Core services (7) and Instruments (7)

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Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Jangi S, Gandhi R, Cox LM, Li N, von Glehn F, Yan R, Patel B, Mazzola MA, Liu S, Glanz BL, Cook S, Tankou S, Stuart F, Melo K, Nejad P, Smith K, Topçuolu BD, Holden J, Kivisäkk P, Chitnis T, De Jager PL, Quintana FJ, Gerber GK, Bry L, Weiner HL. Alterations of the human gut microbiome in multiple sclerosis. Nat Commun. 2016; 7:12015. PMID: 27352007.
    View in: PubMed
  2. Boutin NT, Mathieu K, Hoffnagle AG, Allen NL, Castro VM, Morash M, O'Rourke PP, Hohmann EL, Herring N, Bry L, Slaugenhaupt SA, Karlson EW, Weiss ST, Smoller JW. Implementation of Electronic Consent at a Biobank: An Opportunity for Precision Medicine Research. J Pers Med. 2016; 6(2). PMID: 27294961.
    View in: PubMed
  3. Bucci V, Tzen B, Li N, Simmons M, Tanoue T, Bogart E, Deng L, Yeliseyev V, Delaney ML, Liu Q, Olle B, Stein RR, Honda K, Bry L, Gerber GK. MDSINE: Microbial Dynamical Systems INference Engine for microbiome time-series analyses. Genome Biol. 2016; 17(1):121. PMID: 27259475.
    View in: PubMed
  4. Allegretti JR, Kearney S, Li N, Bogart E, Bullock K, Gerber GK, Bry L, Clish CB, Alm E, Korzenik JR. Recurrent Clostridium difficile infection associates with distinct bile acid and microbiome profiles. Aliment Pharmacol Ther. 2016 Jun; 43(11):1142-53. PMID: 27086647.
    View in: PubMed
  5. Redhu N, Shouval D, Bakthavatchalu V, Wang C, Conaway E, Goettel J, Mack M, Bleich A, Bry L, Fox J, Snapper S, Horwitz B. O-005 YI Microbiota Drives Inflammation by Altering Intestinal Lamina Propria Macrophage Phenotype in a Novel IL10R-Deficient Model of Very Early Onset IBD. Inflamm Bowel Dis. 2016 Mar; 22 Suppl 1:S2-3. PMID: 26849723.
    View in: PubMed
  6. Liu S, da Cunha AP, Rezende RM, Cialic R, Wei Z, Bry L, Comstock LE, Gandhi R, Weiner HL. The Host Shapes the Gut Microbiota via Fecal MicroRNA. Cell Host Microbe. 2016 Jan 13; 19(1):32-43. PMID: 26764595.
    View in: PubMed
  7. Yu DH, Gadkari M, Zhou Q, Yu S, Gao N, Guan Y, Schady D, Roshan TN, Chen MH, Laritsky E, Ge Z, Wang H, Chen R, Westwater C, Bry L, Waterland RA, Moriarty C, Hwang C, Swennes AG, Moore SR, Shen L. Postnatal epigenetic regulation of intestinal stem cells requires DNA methylation and is guided by the microbiome. Genome Biol. 2015; 16(1):211. PMID: 26420038; PMCID: PMC4589031.
  8. Ussar S, Griffin NW, Bezy O, Fujisaka S, Vienberg S, Softic S, Deng L, Bry L, Gordon JI, Kahn CR. Interactions between Gut Microbiota, Host Genetics and Diet Modulate the Predisposition to Obesity and Metabolic Syndrome. Cell Metab. 2015 Sep 1; 22(3):516-30. PMID: 26299453; PMCID: PMC4570502 [Available on 09/01/16].
  9. Pecora ND, Li N, Allard M, Li C, Albano E, Delaney M, Dubois A, Onderdonk AB, Bry L. Genomically Informed Surveillance for Carbapenem-Resistant Enterobacteriaceae in a Health Care System. MBio. 2015; 6(4):e01030. PMID: 26220969; PMCID: PMC4551976.
  10. Wang S, Charbonnier LM, Noval Rivas M, Georgiev P, Li N, Gerber G, Bry L, Chatila TA. MyD88 Adaptor-Dependent Microbial Sensing by Regulatory T Cells Promotes Mucosal Tolerance and Enforces Commensalism. Immunity. 2015 Aug 18; 43(2):289-303. PMID: 26231118; PMCID: PMC4545404 [Available on 08/18/16].
  11. Louis DN, Feldman M, Carter AB, Dighe AS, Pfeifer JD, Bry L, Almeida JS, Saltz J, Braun J, Tomaszewski JE, Gilbertson JR, Sinard JH, Gerber GK, Galli SJ, Golden JA, Becich MJ. Computational Pathology: A Path Ahead. Arch Pathol Lab Med. 2016 Jan; 140(1):41-50. PMID: 26098131.
    View in: PubMed
  12. Yaung SJ, Deng L, Li N, Braff JL, Church GM, Bry L, Wang HH, Gerber GK. Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics. Mol Syst Biol. 2015 Jan; 11(1):788. PMID: 25762151; PMCID: PMC4380924.
  13. Yaung SJ, Deng L, Li N, Braff JL, Church GM, Bry L, Wang HH, Gerber GK. Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics. Mol Syst Biol. 2015 Mar; 11(3):788. PMID: 26148351.
    View in: PubMed
  14. Robb JA, Bry L, Sluss PM, Wagar EA, Kennedy MF. A Call to Standardize Preanalytic Data Elements for Biospecimens, Part II. Arch Pathol Lab Med. 2015 Sep; 139(9):1125-8. PMID: 25594725.
    View in: PubMed
  15. Mietto C, Pinciroli R, Piriyapatsom A, Thomas JG, Bry L, Delaney ML, Du Bois A, Truelove J, Ackman JB, Wojtkiewicz GR, Nahrendorf M, Kacmarek RM, Berra L. Tracheal tube obstruction in mechanically ventilated patients assessed by high-resolution computed tomography. Anesthesiology. 2014 Dec; 121(6):1226-35. PMID: 25254903.
    View in: PubMed
  16. Lyon E, Cockerill FR, Bale SJ, Beadling C, Bry L, Hagenkord J, Kulkarni S, Press R, Palomaki GE. Next generation sequencing in clinical diagnostics: experiences of early adopters. Clin Chem. 2015 Jan; 61(1):41-9. PMID: 25421800.
    View in: PubMed
  17. Louis DN, Gerber GK, Baron JM, Bry L, Dighe AS, Getz G, Higgins JM, Kuo FC, Lane WJ, Michaelson JS, Le LP, Mermel CH, Gilbertson JR, Golden JA. Computational pathology: an emerging definition. Arch Pathol Lab Med. 2014 Sep; 138(9):1133-8. PMID: 25171694.
    View in: PubMed
  18. Aziz N, Zhao Q, Bry L, Driscoll DK, Funke B, Gibson JS, Grody WW, Hegde MR, Hoeltge GA, Leonard DG, Merker JD, Nagarajan R, Palicki LA, Robetorye RS, Schrijver I, Weck KE, Voelkerding KV. College of American Pathologists' laboratory standards for next-generation sequencing clinical tests. Arch Pathol Lab Med. 2015 Apr; 139(4):481-93. PMID: 25152313.
    View in: PubMed
  19. Belzer C, Gerber GK, Roeselers G, Delaney M, DuBois A, Liu Q, Belavusava V, Yeliseyev V, Houseman A, Onderdonk A, Cavanaugh C, Bry L. Dynamics of the microbiota in response to host infection. PLoS One. 2014; 9(7):e95534. PMID: 25014551; PMCID: PMC4094490.
  20. Crawford JM, Bry L, Pfeifer J, Caughron SK, Black-Schaffer S, Kant JA, Kaufman JH. The business of genomic testing: a survey of early adopters. Genet Med. 2014 Dec; 16(12):954-61. PMID: 25010053; PMCID: PMC4262758.
  21. Ghosh P, Vaidya A, Sahoo R, Goldfine A, Herring N, Bry L, Chorev M, Halperin JA. Glycation of the complement regulatory protein CD59 is a novel biomarker for glucose handling in humans. J Clin Endocrinol Metab. 2014 Jun; 99(6):E999-E1006. PMID: 24628556; PMCID: PMC4037733.
  22. Ghosh P, Sahoo R, Vaidya A, Cantel S, Kavishwar A, Goldfine A, Herring N, Bry L, Chorev M, Halperin JA. A specific and sensitive assay for blood levels of glycated CD59: a novel biomarker for diabetes. Am J Hematol. 2013 Aug; 88(8):670-6. PMID: 23670858; PMCID: PMC3775575.
  23. Cebula A, Seweryn M, Rempala GA, Pabla SS, McIndoe RA, Denning TL, Bry L, Kraj P, Kisielow P, Ignatowicz L. Thymus-derived regulatory T cells contribute to tolerance to commensal microbiota. Nature. 2013 May 9; 497(7448):258-62. PMID: 23624374; PMCID: PMC3711137.
  24. Noval Rivas M, Burton OT, Wise P, Zhang YQ, Hobson SA, Garcia Lloret M, Chehoud C, Kuczynski J, DeSantis T, Warrington J, Hyde ER, Petrosino JF, Gerber GK, Bry L, Oettgen HC, Mazmanian SK, Chatila TA. A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis. J Allergy Clin Immunol. 2013 Jan; 131(1):201-12. PMID: 23201093; PMCID: PMC3860814.
  25. Chen L, Chen Z, Baker K, Halvorsen EM, da Cunha AP, Flak MB, Gerber G, Huang YH, Hosomi S, Arthur JC, Dery KJ, Nagaishi T, Beauchemin N, Holmes KV, Ho JW, Shively JE, Jobin C, Onderdonk AB, Bry L, Weiner HL, Higgins DE, Blumberg RS. The short isoform of the CEACAM1 receptor in intestinal T cells regulates mucosal immunity and homeostasis via Tfh cell induction. Immunity. 2012 Nov 16; 37(5):930-46. PMID: 23123061; PMCID: PMC3516394.
  26. Gerber GK, Onderdonk AB, Bry L. Inferring dynamic signatures of microbes in complex host ecosystems. PLoS Comput Biol. 2012; 8(8):e1002624. PMID: 22876171; PMCID: PMC3410865.
  27. Lange NE, Celedón JC, Forno E, Ly NP, Onderdonk A, Bry L, Delaney ML, DuBois AM, Gold DR, Weiss ST, Litonjua AA. Maternal intestinal flora and wheeze in early childhood. Clin Exp Allergy. 2012 Jun; 42(6):901-8. PMID: 22909161; PMCID: PMC3428746.
  28. Dash RC, Robb JA, Booker DL, Foo WC, Witte DL, Bry L. Biospecimens and biorepositories for the community pathologist. Arch Pathol Lab Med. 2012 Jun; 136(6):668-78. PMID: 22646276; PMCID: PMC3679406.
  29. Kurreeman F, Liao K, Chibnik L, Hickey B, Stahl E, Gainer V, Li G, Bry L, Mahan S, Ardlie K, Thomson B, Szolovits P, Churchill S, Murphy SN, Cai T, Raychaudhuri S, Kohane I, Karlson E, Plenge RM. Genetic basis of autoantibody positive and negative rheumatoid arthritis risk in a multi-ethnic cohort derived from electronic health records. Am J Hum Genet. 2011 Jan 7; 88(1):57-69. PMID: 21211616; PMCID: PMC3014362.
  30. Garrett WS, Gallini CA, Yatsunenko T, Michaud M, DuBois A, Delaney ML, Punit S, Karlsson M, Bry L, Glickman JN, Gordon JI, Onderdonk AB, Glimcher LH. Enterobacteriaceae act in concert with the gut microbiota to induce spontaneous and maternally transmitted colitis. Cell Host Microbe. 2010 Sep 16; 8(3):292-300. PMID: 20833380; PMCID: PMC2952357.
  31. Murphy S, Churchill S, Bry L, Chueh H, Weiss S, Lazarus R, Zeng Q, Dubey A, Gainer V, Mendis M, Glaser J, Kohane I. Instrumenting the health care enterprise for discovery research in the genomic era. Genome Res. 2009 Sep; 19(9):1675-81. PMID: 19602638; PMCID: PMC2752136.
  32. Bry, L. BWH Clinical Microbiology Manual for CP Residents. 2009.
  33. Mantis, N., Bry, L. The intestinal epithelium: At the Interface between host and pathogen. Immunity against mucosal pathogens. 2008.
  34. Brown MD, Bry L, Li Z, Sacks DB. Actin pedestal formation by enteropathogenic Escherichia coli is regulated by IQGAP1, calcium, and calmodulin. J Biol Chem. 2008 Dec 12; 283(50):35212-22. PMID: 18809683; PMCID: PMC2596374.
  35. Bry, L. Crimson Project (Word Mark). 2008.
  36. Mantis N., Bry L. The intestinal epithelium: The interface between host and pathogen. 2008.
  37. Baram-Tsbari, A., Sethi, R., Bry, L., Yarden, A. Asking scientists: a decade of questions analyzed by age, gender and country. Science Education. 2008; (In press).
  38. Brown MD, Bry L, Li Z, Sacks DB. IQGAP1 regulates Salmonella invasion through interactions with actin, Rac1, and Cdc42. J Biol Chem. 2007 Oct 12; 282(41):30265-72. PMID: 17693642.
    View in: PubMed
  39. Yoshida M, Kobayashi K, Kuo TT, Bry L, Glickman JN, Claypool SM, Kaser A, Nagaishi T, Higgins DE, Mizoguchi E, Wakatsuki Y, Roopenian DC, Mizoguchi A, Lencer WI, Blumberg RS. Neonatal Fc receptor for IgG regulates mucosal immune responses to luminal bacteria. J Clin Invest. 2006 Aug; 116(8):2142-2151. PMID: 16841095; PMCID: PMC1501111.
  40. Ayelet Baram, Ricky Sethi, Lynn Bry, Anat Yarden. Using questions sent to an Ask-A-Scientist site to identify children's interests in science. Science Education. 2006; 90(6):1050-1072.
  41. Bry L, Brigl M, Brenner MB. CD4+-T-cell effector functions and costimulatory requirements essential for surviving mucosal infection with Citrobacter rodentium. Infect Immun. 2006 Jan; 74(1):673-81. PMID: 16369024; PMCID: PMC1346620.
  42. Bry L. BWH Clinical Microbiology Handbook. 2005. 2005.
  43. Bry L., Vargas SO., McAdam A. The role of Burkholderia cepacia complex infections in patients with cystic fibrosis. CAP Check Survey 2004. 2004; In press.
  44. Bry L. Chapter 12: Prenatal diagnosis and screening of congenital infection. Handbook of Clinical Laboratory Testing During Pregnancy, Ann Gronowski ed. 2004; 245-289.
  45. Lynn Bry. MadSci (Word Mark). 2004.
  46. Bry L, Brenner MB. Critical role of T cell-dependent serum antibody, but not the gut-associated lymphoid tissue, for surviving acute mucosal infection with Citrobacter rodentium, an attaching and effacing pathogen. J Immunol. 2004 Jan 1; 172(1):433-41. PMID: 14688352.
    View in: PubMed
  47. Brigl M, Bry L, Kent SC, Gumperz JE, Brenner MB. Mechanism of CD1d-restricted natural killer T cell activation during microbial infection. Nat Immunol. 2003 Dec; 4(12):1230-7. PMID: 14578883.
    View in: PubMed
  48. Haspel R., Bry L. . Study Guide, HMS Clerkship PA511M.J Introduction to the Clinical Laboratory. 2003.
  49. Ellerin T.B., Bry L. Cavitary pneumonia caused by Rhodococcus equi in an HIV+ man. J. of Clin. Microbiol. 2003; 41(7):iii.
  50. Bry L. BWH Clinical Microbiology Handbook. 2001.
  51. Bry L, Chen PC, Sacks DB. Effects of hemoglobin variants and chemically modified derivatives on assays for glycohemoglobin. Clin Chem. 2001 Feb; 47(2):153-63. PMID: 11159762.
    View in: PubMed
  52. Bry L. Web-based methods for analyzing and processing large volumes of question and answer information. Digital Reference Models for the New Millenium, R. David Lankes, Abby S. Kasowitz Eds. 2000; 54-62.
  53. Bry,\ L. . Study Guide, HMS Clerkship PA511M.J Introduction to the Clinical Laboratory. 1999.
  54. Bry, L. Developing and implementing an Ask-An-Expert Service on the World Wide Web. How to Build and Maintain Digital Reference Services. R. David Lankes and Abby S. Kasowtiz, eds. 1998; 42-48.
  55. Hooper LV, Bry L, Falk PG, Gordon JI. Host-microbial symbiosis in the mammalian intestine: exploring an internal ecosystem. Bioessays. 1998 Apr; 20(4):336-43. PMID: 9619105.
    View in: PubMed
  56. Gordon JI, Hooper LV, McNevin MS, Wong M, Bry L. Epithelial cell growth and differentiation. III. Promoting diversity in the intestine: conversations between the microflora, epithelium, and diffuse GALT. Am J Physiol. 1997 Sep; 273(3 Pt 1):G565-70. PMID: 9316460.
    View in: PubMed
  57. Bry L, Falk PG, Midtvedt T, Gordon JI. A model of host-microbial interactions in an open mammalian ecosystem. Science. 1996 Sep 6; 273(5280):1380-3. PMID: 8703071.
    View in: PubMed
  58. Lynn Bry. Tansgenic and Gnotobiotic Models of Host-Microbial Interactions in the Gut. 1996.
  59. Bry L, Falk PG, Gordon JL. Genetic engineering of carbohydrate biosynthetic pathways in transgenic mice demonstrates cell cycle-associated regulation of glycoconjugate production in small intestinal epithelial cells. Proc Natl Acad Sci U S A. 1996 Feb 6; 93(3):1161-6. PMID: 8577733; PMCID: PMC40049.
  60. Rubio CA, Kanter L, Björk J, Poppen B, Bry L. Paneth cell-rich flat adenoma of the rectum: report of a case. Jpn J Cancer Res. 1996 Jan; 87(1):109-12. PMID: 8609042.
    View in: PubMed
  61. Bry L, Sethi R, Kelly K, et al. MadSci Network: www.madsci.org. 1995.
  62. Falk PG, Bry L, Holgersson J, Gordon JI. Expression of a human alpha-1,3/4-fucosyltransferase in the pit cell lineage of FVB/N mouse stomach results in production of Leb-containing glycoconjugates: a potential transgenic mouse model for studying Helicobacter pylori infection. Proc Natl Acad Sci U S A. 1995 Feb 28; 92(5):1515-9. PMID: 7878011; PMCID: PMC42550.
  63. Bry L, Falk P, Huttner K, Ouellette A, Midtvedt T, Gordon JI. Paneth cell differentiation in the developing intestine of normal and transgenic mice. Proc Natl Acad Sci U S A. 1994 Oct 25; 91(22):10335-9. PMID: 7937951; PMCID: PMC45014.
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