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Priscilla L. Yang, Ph.D.

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Overview
The focus of my research group is on the study of mammalian viruses that are significant causes of morbidity and mortality in humans, in particular hepatitis B virus (HBV), hepatitis C virus (HCV), and dengue virus (DENV). I have been especially interested in the development of a variety of chemical tools to characterize novel interactions between viral pathogens and their hosts at both the molecular and pathway levels.

First, I have led successful efforts in developing and using immunofluorescence image-based screens to identify both small molecules and shRNAs that perturb dengue virus replication. As we follow-up on our original kinase inhibitor screens, a significant portion of my group is currently focused on the characterization of the mechanisms by which Src, Abl, and other specific kinases regulate the dengue virus replication cycle.

Second, my group has developed small molecule inhibitors of dengue virus entry and used these as mechanistic probes in cellular and biochemical studies of the dengue virus entry. We have discovered inhibitors that can simultaneously inhibit two different steps of the dengue virus replication cycle through interactions with two distinct targets, one a host kinase and the other the envelope protein coating the virion surface.

Third, my group has developed global liquid chromatography - mass spectrometry based profiling as a way to identify host lipids and lipid metabolic pathways that are important for the replication of viral pathogens. In our initial work, we have focused on profiling HBV and HCV, two phylogenetically unrelated viruses that cause chronic infections in the liver. This work has led to the identification of virus-specific perturbations in sterol metabolism that promote viral replication. My group is actively investigating the mechanisms responsible for these perturbations as well as their biological significance in the viral life cycle and pathogenesis. In addition, we are performing comparative lipid metabolite profiling of additional viral pathogens to identify common pathways utilized by viruses that cause chronic versus acute infections and viruses that infect the liver versus those that infect other cells and tissues.

The members of my group come from diverse backgrounds including chemistry, biochemistry, cell biology, and virology. My group has the chemical and virological expertise as well as the instrumentation, collaborations, and institutional support to use these tools to study the basic virology and pathogenesis of these human viral pathogens. Our work with hepatitis B and C viruses as well as our work using small molecules to interrogate the signal transduction pathways of host protein kinases are geared towards understanding the role of viral pathogens in cancer biology.

Mentoring
Creating an in vivo Murine Model of Hepatitis C Virus Through Directed Evolution
Summer, 06/20/05 - 08/19/05
Creating a mouse model for Hepatitis C Virus (HCV) using a chemical selection
Summer, 06/01/06 - 09/01/05

Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R01AI146152 (YANG, PRISCILLA LI-NING) Mar 1, 2020 - Feb 28, 2025
    NIH/NIAID
    Validating the Flavivirus Envelope Protein as an Antiviral Target
    Role: Principal Investigator
  2. R21AI148972 (YANG, PRISCILLA LI-NING) Jan 1, 2020 - Dec 31, 2021
    NIH/NIAID
    How Hepatitis C Virus Regulates Desmosterol to Affect RNA Replication: a New Virus-Host Interaction
    Role: Principal Investigator
  3. U19AI109740 (WHELAN, SEAN PJ) Mar 1, 2014 - Feb 28, 2019
    NIH/NIAID
    Small molecule inhibitors of enveloped virus entry
    Role: Co-Principal Investigator
  4. R01AI095499 (YANG, PRISCILLA LI-NING) Nov 20, 2013 - Oct 31, 2016
    NIH/NIAID
    Chemical Tools for the Study of Dengue Virus Entry
    Role: Co-Principal Investigator
  5. R56AI095499 (YANG, PRISCILLA LI-NING) Sep 30, 2011 - Nov 19, 2013
    NIH/NIAID
    Chemical Tools for the Study of Dengue Virus Entry
    Role: Principal Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Ramanathan HN, Zhang S, Douam F, Mar KB, Chang J, Yang PL, Schoggins JW, Ploss A, Lindenbach BD. A Sensitive Yellow Fever Virus Entry Reporter Identifies Valosin-Containing Protein (VCP/p97) as an Essential Host Factor for Flavivirus Uncoating. mBio. 2020 04 14; 11(2). PMID: 32291299.
    Citations:    
  2. de Wispelaere M, Carocci M, Burri DJ, Neidermyer WJ, Olson CM, Roggenbach I, Liang Y, Wang J, Whelan SPJ, Gray NS, Yang PL. A broad-spectrum antiviral molecule, QL47, selectively inhibits eukaryotic translation. J Biol Chem. 2020 Feb 07; 295(6):1694-1703. PMID: 31914414.
    Citations:    
  3. Lian W, Jang J, Potisopon S, Li PC, Rahmeh A, Wang J, Kwiatkowski NP, Gray NS, Yang PL. Correction to Discovery of Immunologically Inspired Small Molecules That Target the Viral Envelope Protein. ACS Infect Dis. 2019 Dec 13; 5(12):2176. PMID: 31675213.
    Citations:    
  4. de Wispelaere M, Du G, Donovan KA, Zhang T, Eleuteri NA, Yuan JC, Kalabathula J, Nowak RP, Fischer ES, Gray NS, Yang PL. Small molecule degraders of the hepatitis C virus protease reduce susceptibility to resistance mutations. Nat Commun. 2019 08 01; 10(1):3468. PMID: 31371704.
    Citations:    
  5. Pitts J, Hsia CY, Lian W, Wang J, Pfeil MP, Kwiatkowski N, Li Z, Jang J, Gray NS, Yang PL. Identification of small molecule inhibitors targeting the Zika virus envelope protein. Antiviral Res. 2019 04; 164:147-153. PMID: 30771406.
    Citations:    
  6. Schmidt AG, Lee K, Yang PL, Harrison SC. Correction: Small-Molecule Inhibitors of Dengue-Virus Entry. PLoS Pathog. 2019 01; 15(1):e1007553. PMID: 30703168.
    Citations:    Fields:    
  7. Li PC, Jang J, Hsia CY, Groomes PV, Lian W, de Wispelaere M, Pitts JD, Wang J, Kwiatkowski N, Gray NS, Yang PL. Small Molecules Targeting the Flavivirus E Protein with Broad-Spectrum Activity and Antiviral Efficacy in Vivo. ACS Infect Dis. 2019 03 08; 5(3):460-472. PMID: 30608640.
    Citations:    Fields:    
  8. Kaushansky A, Hedstrom L, Goldman A, Singh J, Yang PL, Rathod PK, Cynamon M, Wodarz D, Mahadevan D, Tomaras A, Navia MA, Schiffer CA. A call to arms: Unifying the fight against resistance. Sci Signal. 2018 10 23; 11(553). PMID: 30352947.
    Citations:    Fields:    
  9. Lian W, Jang J, Potisopon S, Li PC, Rahmeh A, Wang J, Kwiatkowski NP, Gray NS, Yang PL. Discovery of Immunologically Inspired Small Molecules That Target the Viral Envelope Protein. ACS Infect Dis. 2018 09 14; 4(9):1395-1406. PMID: 30027735.
    Citations:    Fields:    
  10. Chao LH, Jang J, Johnson A, Nguyen A, Gray NS, Yang PL, Harrison SC. How small-molecule inhibitors of dengue-virus infection interfere with viral membrane fusion. Elife. 2018 07 12; 7. PMID: 29999491.
    Citations:    Fields:    
  11. de Wispelaere M, Lian W, Potisopon S, Li PC, Jang J, Ficarro SB, Clark MJ, Zhu X, Kaplan JB, Pitts JD, Wales TE, Wang J, Engen JR, Marto JA, Gray NS, Yang PL. Inhibition of Flaviviruses by Targeting a Conserved Pocket on the Viral Envelope Protein. Cell Chem Biol. 2018 08 16; 25(8):1006-1016.e8. PMID: 29937406.
    Citations: 1     Fields:    
  12. Pitts JD, Li PC, de Wispelaere M, Yang PL. Antiviral activity of N-(4-hydroxyphenyl) retinamide (4-HPR) against Zika virus. Antiviral Res. 2017 Nov; 147:124-130. PMID: 29051080.
    Citations: 1     Fields:    Translation:HumansAnimalsCells
  13. Liang Y, de Wispelaere M, Carocci M, Liu Q, Wang J, Yang PL, Gray NS. Structure-Activity Relationship Study of QL47: A Broad-Spectrum Antiviral Agent. ACS Med Chem Lett. 2017 Mar 09; 8(3):344-349. PMID: 28337328.
    Citations:    
  14. de Wispelaere M, Carocci M, Liang Y, Liu Q, Sun E, Vetter ML, Wang J, Gray NS, Yang PL. Discovery of host-targeted covalent inhibitors of dengue virus. Antiviral Res. 2017 Mar; 139:171-179. PMID: 28034743.
    Citations: 3     Fields:    Translation:HumansCells
  15. Costello DA, Villareal VA, Yang PL. Desmosterol Increases Lipid Bilayer Fluidity during Hepatitis C Virus Infection. ACS Infect Dis. 2016 11 11; 2(11):852-862. PMID: 27933788.
    Citations: 1     Fields:    Translation:HumansCells
  16. Villareal VA, Fu D, Costello DA, Xie XS, Yang PL. Hepatitis C Virus Selectively Alters the Intracellular Localization of Desmosterol. ACS Chem Biol. 2016 07 15; 11(7):1827-33. PMID: 27128812.
    Citations: 2     Fields:    Translation:HumansCells
  17. Clark MJ, Miduturu C, Schmidt AG, Zhu X, Pitts JD, Wang J, Potisopon S, Zhang J, Wojciechowski A, Hann Chu JJ, Gray NS, Yang PL. GNF-2 Inhibits Dengue Virus by Targeting Abl Kinases and the Viral E Protein. Cell Chem Biol. 2016 04 21; 23(4):443-52. PMID: 27105280.
    Citations: 6     Fields:    Translation:HumansAnimalsCells
  18. Chou YY, Cuevas C, Carocci M, Stubbs SH, Ma M, Cureton DK, Chao L, Evesson F, He K, Yang PL, Whelan SP, Ross SR, Kirchhausen T, Gaudin R. Identification and Characterization of a Novel Broad-Spectrum Virus Entry Inhibitor. J Virol. 2016 May; 90(9):4494-4510. PMID: 26912630.
    Citations: 2     Fields:    Translation:HumansAnimalsCells
  19. Carocci M, Yang PL. Lactimidomycin is a broad-spectrum inhibitor of dengue and other RNA viruses. Antiviral Res. 2016 Apr; 128:57-62. PMID: 26872864.
    Citations: 3     Fields:    Translation:HumansAnimalsCells
  20. Villareal VA, Rodgers MA, Costello DA, Yang PL. Targeting host lipid synthesis and metabolism to inhibit dengue and hepatitis C viruses. Antiviral Res. 2015 Dec; 124:110-21. PMID: 26526588.
    Citations: 7     Fields:    Translation:HumansCells
  21. Inci F, Filippini C, Baday M, Ozen MO, Calamak S, Durmus NG, Wang S, Hanhauser E, Hobbs KS, Juillard F, Kuang PP, Vetter ML, Carocci M, Yamamoto HS, Takagi Y, Yildiz UH, Akin D, Wesemann DR, Singhal A, Yang PL, Nibert ML, Fichorova RN, Lau DT, Henrich TJ, Kaye KM, Schachter SC, Kuritzkes DR, Steinmetz LM, Gambhir SS, Davis RW, Demirci U. Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE2RD) for diagnostics. Proc Natl Acad Sci U S A. 2015 Aug 11; 112(32):E4354-63. PMID: 26195743.
    Citations: 7     Fields:    Translation:HumansCells
  22. Carocci M, Hinshaw SM, Rodgers MA, Villareal VA, Burri DJ, Pilankatta R, Maharaj NP, Gack MU, Stavale EJ, Warfield KL, Yang PL. The bioactive lipid 4-hydroxyphenyl retinamide inhibits flavivirus replication. Antimicrob Agents Chemother. 2015 Jan; 59(1):85-95. PMID: 25313218.
    Citations: 9     Fields:    Translation:HumansAnimalsCells
  23. Vetter ML, Zhang Z, Liu S, Wang J, Cho H, Zhang J, Zhang W, Gray NS, Yang PL. Fluorescent visualization of Src by using dasatinib-BODIPY. Chembiochem. 2014 Jun 16; 15(9):1317-24. PMID: 24828915.
    Citations: 5     Fields:    Translation:HumansAnimalsCells
  24. de Wispelaere M, LaCroix AJ, Yang PL. The small molecules AZD0530 and dasatinib inhibit dengue virus RNA replication via Fyn kinase. J Virol. 2013 Jul; 87(13):7367-81. PMID: 23616652.
    Citations: 19     Fields:    Translation:HumansCells
  25. Vetter ML, Rodgers MA, Patricelli MP, Yang PL. Chemoproteomic profiling identifies changes in DNA-PK as markers of early dengue virus infection. ACS Chem Biol. 2012 Dec 21; 7(12):2019-26. PMID: 22999307.
    Citations: 3     Fields:    Translation:HumansCells
  26. Zhang Z, Kwiatkowski N, Zeng H, Lim SM, Gray NS, Zhang W, Yang PL. Leveraging kinase inhibitors to develop small molecule tools for imaging kinases by fluorescence microscopy. Mol Biosyst. 2012 Oct; 8(10):2523-6. PMID: 22673640.
    Citations: 6     Fields:    Translation:HumansCells
  27. de Wispelaere M, Yang PL. Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly. J Virol. 2012 Jul; 86(13):7072-83. PMID: 22532681.
    Citations: 7     Fields:    Translation:HumansCells
  28. Rodgers MA, Villareal VA, Schaefer EA, Peng LF, Corey KE, Chung RT, Yang PL. Lipid metabolite profiling identifies desmosterol metabolism as a new antiviral target for hepatitis C virus. J Am Chem Soc. 2012 Apr 25; 134(16):6896-9. PMID: 22480142.
    Citations: 11     Fields:    Translation:HumansCells
  29. Schmidt AG, Lee K, Yang PL, Harrison SC. Small-molecule inhibitors of dengue-virus entry. PLoS Pathog. 2012; 8(4):e1002627. PMID: 22496653.
    Citations: 16     Fields:    Translation:HumansAnimalsCells
  30. Yang PL, Gao M, Lin K, Liu Q, Villareal VA. Anti-HCV drugs in the pipeline. Curr Opin Virol. 2011 Dec; 1(6):607-16. PMID: 22440918.
    Citations: 13     Fields:    Translation:HumansAnimalsCells
  31. Schmidt AG, Yang PL, Harrison SC. Peptide inhibitors of flavivirus entry derived from the E protein stem. J Virol. 2010 Dec; 84(24):12549-54. PMID: 20881042.
    Citations: 18     Fields:    Translation:AnimalsCells
  32. Schmidt AG, Yang PL, Harrison SC. Peptide inhibitors of dengue-virus entry target a late-stage fusion intermediate. PLoS Pathog. 2010 Apr 08; 6(4):e1000851. PMID: 20386713.
    Citations: 40     Fields:    Translation:AnimalsCells
  33. Yang PL, Althage A, Chung J, Maier H, Wieland S, Isogawa M, Chisari FV. Immune effectors required for hepatitis B virus clearance. Proc Natl Acad Sci U S A. 2010 Jan 12; 107(2):798-802. PMID: 20080755.
    Citations: 73     Fields:    Translation:HumansAnimalsCells
  34. Sessions OM, Barrows NJ, Souza-Neto JA, Robinson TJ, Hershey CL, Rodgers MA, Ramirez JL, Dimopoulos G, Yang PL, Pearson JL, Garcia-Blanco MA. Discovery of insect and human dengue virus host factors. Nature. 2009 Apr 23; 458(7241):1047-50. PMID: 19396146.
    Citations: 161     Fields:    Translation:HumansAnimalsCells
  35. Rodgers MA, Saghatelian A, Yang PL. Identification of an overabundant cholesterol precursor in hepatitis B virus replicating cells by untargeted lipid metabolite profiling. J Am Chem Soc. 2009 Apr 15; 131(14):5030-1. PMID: 19301856.
    Citations: 14     Fields:    Translation:HumansCells
  36. Zhang J, Yang PL, Gray NS. Targeting cancer with small molecule kinase inhibitors. Nat Rev Cancer. 2009 Jan; 9(1):28-39. PMID: 19104514.
    Citations: 576     Fields:    Translation:HumansCells
  37. Hwang YC, Chu JJ, Yang PL, Chen W, Yates MV. Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay. Antiviral Res. 2008 Mar; 77(3):232-6. PMID: 18243348.
    Citations: 9     Fields:    Translation:AnimalsCells
  38. Chu JJ, Yang PL. c-Src protein kinase inhibitors block assembly and maturation of dengue virus. Proc Natl Acad Sci U S A. 2007 Feb 27; 104(9):3520-5. PMID: 17360676.
    Citations: 34     Fields:    Translation:HumansAnimalsCells
  39. Yang PL, Althage A, Chung J, Chisari FV. Hydrodynamic injection of viral DNA: a mouse model of acute hepatitis B virus infection. Proc Natl Acad Sci U S A. 2002 Oct 15; 99(21):13825-30. PMID: 12374864.
    Citations: 109     Fields:    Translation:HumansAnimalsCells
  40. Gildersleeve J, Janes J, Ulrich H, Yang P, Barbas C, Schultz PG. Development of a genetic selection for catalytic antibodies. Bioorg Med Chem Lett. 2002 Jun 17; 12(12):1691-4. PMID: 12039592.
    Citations:    Fields:    Translation:Cells
  41. P.L. Yang, A. Althage, J.S. Chung, F.V. Chisari. Hydrodynamic transfection: a new model for acute hepatitis B virus infection. Proc Natl Acad Sci USA. 2002; 99(21):13825-13830.
  42. Yin J, Mundorff EC, Yang PL, Wendt KU, Hanway D, Stevens RC, Schultz PG. A comparative analysis of the immunological evolution of antibody 28B4. Biochemistry. 2001 Sep 11; 40(36):10764-73. PMID: 11535051.
    Citations: 15     Fields:    Translation:AnimalsCells
  43. Yang PL, Schultz PG. Mutational analysis of the affinity maturation of antibody 48G7. J Mol Biol. 1999 Dec 17; 294(5):1191-201. PMID: 10600377.
    Citations: 10     Fields:    Translation:HumansAnimalsCells
  44. Priscilla Li-Ning Yang, Ph.D. Structure, Function, and Evolution of Catalytic Antibodies [dissertation]. 1999.
  45. P.A. Patten, N.S. Gray, P.L. Yang, C.B. Marks, G. J. Wedemayer, J.J. Boniface, R.C. Stevens, P.G. Schultz. The immunological evolution of catalysis. Science (Washington D C). 1996; 271(5252):1086-1091.
  46. Ulrich HD, Patten PA, Yang PL, Romesberg FE, Schultz PG. Expression studies of catalytic antibodies. Proc Natl Acad Sci U S A. 1995 Dec 05; 92(25):11907-11. PMID: 8524873.
    Citations: 11     Fields:    Translation:HumansAnimalsCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.