Harvard Catalyst Profiles

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Dyann Fergus Wirth, Ph.D.

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Massachusetts Institute of TechnologyPh.D.1978
University of Wisconsin , MadisonB.A.
1972 - 1973
1973 - 1978
Predoctoral Fellowship
Award in Molecular Parasitology
1985 - 1990
Award in Molecular Parasitology
Bailey K. Ashford Award
Bailey K. Ashford Award
1998 - 1999
Joseph Augustin LePrince Medal
Faculty Mentoring Award
Lifetime Achievement Award

Protozoan parasites remain major causes of disease in developing countries throughout the world, yet little is known about the biology or molecular biology of these organisms. The long term goal of this work is to understand basic molecular mechanisms in protozoan parasites with the goal of discovering and applying parasite specific interventions.

The approach my laboratory has taken is to develop methods for molecular genetic manipulation of protozoan parasites in order to begin functional analysis of genes important for parasite virulence, with an emphasis of mechanisms of drug resistance in parasites. Drug resistance poses a particularly difficult problem in developing countries where newer chemotherapeutic agents are often unavailable or too expensive for routine use. Drug resistance is particularly acute in malaria where resistant parasites have spread throughout the endemic world.

Recent evidence from my laboratory and from several other groups worldwide has suggested that a major mechanism of drug resistance in protozoan parasites may be through the expression of a P-glycoprotein type molecule encoded by a multi-drug resistance gene. Genes related to mammalian multi-drug resistance genes have been identified in several protozoan parasites and overexpression of these genes is associated with drug resistance. The immediate goal of the research is to test the hypothesis that these mdr-like genes are indeed the cause of drug resistance in protozoan parasites. This work has been initiated in the Leishmania parasite where my laboratory has previously developed a transfection system, and thus the role of the Leishmania enriettii mdr-like genes can be directly tested.

In parallel, my laboratory has recently developed an analogous transfection system for the malaria parasite. By analyzing these two protozoan systems in parallel, one where the molecular tools are readily available and the other in which drug resistant parasites represent a major and immediate threat to world health, I hope both to understand the mechanism of drug resistance in protozoan parasites and to use this information to develop new approaches to either preventing or reversing drug resistance in these organisms.

Executive Assistant: Jessica Gard, jgard@hsph.harvard.edu

The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R01AI143723 (MAZITSCHEK, RALPH) Apr 1, 2020 - Mar 31, 2025
    Proline homeostasis: a novel mediator of drug tolerance in Plasmodium falciparum
    Role: Co-Principal Investigator
  2. R21AI117304 (WIRTH, DYANN F) Feb 17, 2016 - Jan 31, 2019
    Defining physiological correlates of the human malaria infectious reservoir
    Role: Principal Investigator
  3. R01AI099105 (WIRTH, DYANN F) Apr 5, 2013 - Jun 30, 2022
    Novel Genetic Mechanism of Artemisinin Resistance for Malaria