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Randall W. King, M.D., Ph.D.

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Mentoring
Ubiquitination in Xenopus egg extract
Summer, 06/01/06 - 08/31/06

Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R35GM127032 (KING, RANDALL W) Apr 1, 2018 - Mar 31, 2023
    NIH/NIGMS
    Mechanistic Analysis of the Ubiquitin-Proteasome System
    Role: Principal Investigator
  2. R01GM066492 (KING, RANDALL W) May 1, 2002 - Nov 30, 2016
    NIH/NIGMS
    Chemical Genetic and Biochemical Studies of Mitotic Proteolysis
    Role: Principal Investigator
  3. R56GM066492 (KING, RANDALL W) May 1, 2002 - Apr 30, 2008
    NIH/NIGMS
    Chemical Genetic and Biochmechical Studies of Mitotic Proteolysis
    Role: Principal Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Boselli M, Lee BH, Robert J, Prado MA, Min SW, Cheng C, Silva MC, Seong C, Elsasser S, Hatle KM, Gahman TC, Gygi SP, Haggarty SJ, Gan L, King RW, Finley D. An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons. J Biol Chem. 2017 11 24; 292(47):19209-19225. PMID: 28972160.
    Citations: 9     Fields:    Translation:AnimalsCells
  2. Lee BH, Lu Y, Prado MA, Shi Y, Tian G, Sun S, Elsasser S, Gygi SP, King RW, Finley D. USP14 deubiquitinates proteasome-bound substrates that are ubiquitinated at multiple sites. Nature. 2016 Apr 21; 532(7599):398-401. PMID: 27074503.
    Citations: 29     Fields:    Translation:HumansAnimalsCells
  3. Lub S, Maes A, Maes K, De Veirman K, De Bruyne E, Menu E, Fostier K, Kassambara A, Moreaux J, Hose D, Leleu X, King RW, Vanderkerken K, Van Valckenborgh E. Inhibiting the anaphase promoting complex/cyclosome induces a metaphase arrest and cell death in multiple myeloma cells. Oncotarget. 2016 Jan 26; 7(4):4062-76. PMID: 26716651.
    Citations: 4     Fields:    Translation:HumansCells
  4. de Lange J, Faramarz A, Oostra AB, de Menezes RX, van der Meulen IH, Rooimans MA, Rockx DA, Brakenhoff RH, van Beusechem VW, King RW, de Winter JP, Wolthuis RMF. Defective sister chromatid cohesion is synthetically lethal with impaired APC/C function. Nat Commun. 2015 Oct 01; 6:8399. PMID: 26423134.
    Citations: 8     Fields:    Translation:HumansCells
  5. Lu Y, Lee BH, King RW, Finley D, Kirschner MW. Substrate degradation by the proteasome: a single-molecule kinetic analysis. Science. 2015 Apr 10; 348(6231):1250834. PMID: 25859050.
    Citations: 46     Fields:    Translation:HumansCells
  6. King RW, Finley D. Sculpting the proteome with small molecules. Nat Chem Biol. 2014 Nov; 10(11):870-4. PMID: 25325686.
    Citations: 5     Fields:    Translation:HumansAnimalsCells
  7. Sackton KL, Dimova N, Zeng X, Tian W, Zhang M, Sackton TB, Meaders J, Pfaff KL, Sigoillot F, Yu H, Luo X, King RW. Synergistic blockade of mitotic exit by two chemical inhibitors of the APC/C. Nature. 2014 Oct 30; 514(7524):646-9. PMID: 25156254.
    Citations: 47     Fields:    Translation:Cells
  8. Lim HJ, Dimova NV, Tan MK, Sigoillot FD, King RW, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013 Nov; 33(21):4166-80. PMID: 23979597.
    Citations: 17     Fields:    Translation:HumansCells
  9. Pfaff KL, King RW. Determinants of human cyclin B1 association with mitotic chromosomes. PLoS One. 2013; 8(3):e59169. PMID: 23505570.
    Citations: 2     Fields:    Translation:HumansCells
  10. Lee BH, Finley D, King RW. A High-Throughput Screening Method for Identification of Inhibitors of the Deubiquitinating Enzyme USP14. Curr Protoc Chem Biol. 2012 Dec 01; 4(4):311-30. PMID: 23788557.
    Citations: 9     Fields:    
  11. Zeng X, King RW. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. Nat Chem Biol. 2012 Feb 26; 8(4):383-92. PMID: 22366722.
    Citations: 19     Fields:    Translation:AnimalsCells
  12. Adamson B, Smogorzewska A, Sigoillot FD, King RW, Elledge SJ. A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Nat Cell Biol. 2012 Feb 19; 14(3):318-28. PMID: 22344029.
    Citations: 133     Fields:    Translation:HumansCells
  13. Sigoillot FD, Lyman S, Huckins JF, Adamson B, Chung E, Quattrochi B, King RW. A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens. Nat Methods. 2012 Feb 19; 9(4):363-6. PMID: 22343343.
    Citations: 68     Fields:    Translation:HumansAnimalsCells
  14. Dimova NV, Hathaway NA, Lee BH, Kirkpatrick DS, Berkowitz ML, Gygi SP, Finley D, King RW. APC/C-mediated multiple monoubiquitylation provides an alternative degradation signal for cyclin B1. Nat Cell Biol. 2012 Jan 29; 14(2):168-76. PMID: 22286100.
    Citations: 54     Fields:    Translation:HumansAnimalsCells
  15. Sigoillot FD, Huckins JF, Li F, Zhou X, Wong ST, King RW. A time-series method for automated measurement of changes in mitotic and interphase duration from time-lapse movies. PLoS One. 2011; 6(9):e25511. PMID: 21966537.
    Citations: 6     Fields:    Translation:HumansCells
  16. Miduturu CV, Deng X, Kwiatkowski N, Yang W, Brault L, Filippakopoulos P, Chung E, Yang Q, Schwaller J, Knapp S, King RW, Lee JD, Herrgard S, Zarrinkar P, Gray NS. High-throughput kinase profiling: a more efficient approach toward the discovery of new kinase inhibitors. Chem Biol. 2011 Jul 29; 18(7):868-79. PMID: 21802008.
    Citations: 29     Fields:    Translation:Humans
  17. Harper JW, King RW. Stuck in the middle: drugging the ubiquitin system at the e2 step. Cell. 2011 Jun 24; 145(7):1007-9. PMID: 21703444.
    Citations: 3     Fields:    
  18. Krajcovic M, Johnson NB, Sun Q, Normand G, Hoover N, Yao E, Richardson AL, King RW, Cibas ES, Schnitt SJ, Brugge JS, Overholtzer M. A non-genetic route to aneuploidy in human cancers. Nat Cell Biol. 2011 Mar; 13(3):324-30. PMID: 21336303.
    Citations: 53     Fields:    Translation:HumansCells
  19. Sigoillot FD, King RW. Vigilance and validation: Keys to success in RNAi screening. ACS Chem Biol. 2011 Jan 21; 6(1):47-60. PMID: 21142076.
    Citations: 54     Fields:    Translation:Cells
  20. Zeng X, Sigoillot F, Gaur S, Choi S, Pfaff KL, Oh DC, Hathaway N, Dimova N, Cuny GD, King RW. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. Cancer Cell. 2010 Oct 19; 18(4):382-95. PMID: 20951947.
    Citations: 105     Fields:    Translation:HumansAnimalsCells
  21. Lee BH, Lee MJ, Park S, Oh DC, Elsasser S, Chen PC, Gartner C, Dimova N, Hanna J, Gygi SP, Wilson SM, King RW, Finley D. Enhancement of proteasome activity by a small-molecule inhibitor of USP14. Nature. 2010 Sep 09; 467(7312):179-84. PMID: 20829789.
    Citations: 277     Fields:    Translation:HumansAnimalsCells
  22. Lee MJ, Lee BH, Hanna J, King RW, Finley D. Trimming of ubiquitin chains by proteasome-associated deubiquitinating enzymes. Mol Cell Proteomics. 2011 May; 10(5):R110.003871. PMID: 20823120.
    Citations: 83     Fields:    Translation:HumansAnimals
  23. Normand G, King RW. Understanding cytokinesis failure. Adv Exp Med Biol. 2010; 676:27-55. PMID: 20687468.
    Citations: 29     Fields:    Translation:HumansAnimalsCells
  24. King RW. When 2+2=5: the origins and fates of aneuploid and tetraploid cells. Biochim Biophys Acta. 2008 Sep; 1786(1):4-14. PMID: 18703117.
    Citations: 42     Fields:    Translation:HumansAnimalsCells
  25. Normand G, King RW. Understanding Cytokinesis Failure. Polyploidization and Cancer, Randy Poon, Ed. 2008; In press.
  26. King, RW. When 2+2=5: The Origins and Fates of Aneuploid and Tetraploid Cells. Biochem Biophys Acta Reviews on Cancer. 2008; In press.
  27. Overholtzer M, Mailleux AA, Mouneimne G, Normand G, Schnitt SJ, King RW, Cibas ES, Brugge JS. A nonapoptotic cell death process, entosis, that occurs by cell-in-cell invasion. Cell. 2007 Nov 30; 131(5):966-79. PMID: 18045538.
    Citations: 153     Fields:    Translation:HumansCells
  28. Kleijnen MF, Roelofs J, Park S, Hathaway NA, Glickman M, King RW, Finley D. Stability of the proteasome can be regulated allosterically through engagement of its proteolytic active sites. Nat Struct Mol Biol. 2007 Dec; 14(12):1180-8. PMID: 18026118.
    Citations: 67     Fields:    Translation:Cells
  29. Wang M, Zhou X, Li F, Huckins J, King RW, Wong ST. Novel cell segmentation and online SVM for cell cycle phase identification in automated microscopy. Bioinformatics. 2008 Jan 01; 24(1):94-101. PMID: 17989093.
    Citations: 36     Fields:    Translation:Cells
  30. Bentley AM, Normand G, Hoyt J, King RW. Distinct sequence elements of cyclin B1 promote localization to chromatin, centrosomes, and kinetochores during mitosis. Mol Biol Cell. 2007 Dec; 18(12):4847-58. PMID: 17881737.
    Citations: 22     Fields:    Translation:HumansAnimalsCells
  31. Wang M, Zhou X, King RW, Wong ST. Context based mixture model for cell phase identification in automated fluorescence microscopy. BMC Bioinformatics. 2007; 30:8-32.
  32. Crosas B, Hanna J, Kirkpatrick DS, Zhang DP, Tone Y, Hathaway NA, Buecker C, Leggett DS, Schmidt M, King RW, Gygi SP, Finley D. Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell. 2006 Dec 29; 127(7):1401-13. PMID: 17190603.
    Citations: 128     Fields:    Translation:Animals
  33. Hanna J, Hathaway NA, Tone Y, Crosas B, Elsasser S, Kirkpatrick DS, Leggett DS, Gygi SP, King RW, Finley D. Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation. Cell. 2006 Oct 06; 127(1):99-111. PMID: 17018280.
    Citations: 135     Fields:    Translation:HumansAnimals
  34. Kirkpatrick DS, Hathaway NA, Hanna J, Elsasser S, Rush J, Finley D, King RW, Gygi SP. Quantitative analysis of in vitro ubiquitinated cyclin B1 reveals complex chain topology. Nat Cell Biol. 2006 Jul; 8(7):700-10. PMID: 16799550.
    Citations: 171     Fields:    Translation:AnimalsCells
  35. Stern HM, Murphey RD, Shepard JL, Amatruda JF, Straub CT, Pfaff KL, Weber G, Tallarico JA, King RW, Zon LI. Small molecules that delay S phase suppress a zebrafish bmyb mutant. Nat Chem Biol. 2005 Dec; 1(7):366-70. PMID: 16372403.
    Citations: 59     Fields:    Translation:AnimalsCells
  36. Shi Q, King RW. Chromosome nondisjunction yields tetraploid rather than aneuploid cells in human cell lines. Nature. 2005 Oct 13; 437(7061):1038-42. PMID: 16222248.
    Citations: 120     Fields:    Translation:HumansCells
  37. Lorang J, King RW. Cytological profiling: providing more haystacks for chemists' needles. Genome Biol. 2005; 6(8):228. PMID: 16086858.
    Citations: 1     Fields:    Translation:Cells
  38. Hathaway NA, King RW. Dissecting cell biology with chemical scalpels. Curr Opin Cell Biol. 2005 Feb; 17(1):12-9. PMID: 15661514.
    Citations:    Fields:    Translation:HumansAnimalsCells
  39. Hoyt J, King RW. Adenoviral expression of reporter proteins for high-throughput cell-based screening. Methods Mol Biol. 2005; 310:187-95. PMID: 16353377.
    Citations: 1     Fields:    Translation:HumansAnimalsCells
  40. Stern HM, Murphey RD, Shepard JL, Amatruda JF, Straub CT, Pfaff KL, Weber G, Tallarico JA, King RW, Zon LI. Persynthamide suppresses bmyb-associated cell cycle defects via the intra-S checkpoint. Nature Chem. Biol. 2005; 1:366-370.
  41. Salic A, King RW. Identifying small molecule inhibitors of the ubiquitin-proteasome pathway in Xenopus egg extracts. Methods Enzymol. 2005; 399:567-85. PMID: 16338382.
    Citations: 5     Fields:    Translation:AnimalsCells
  42. Lorang J, King RW. Cytological profiling: Identifying more haystacks for chemists' needles. 2005; 6:228.
  43. Tochtrop GP, King RW. Target identification strategies in chemical genetics. Comb Chem High Throughput Screen. 2004 Nov; 7(7):677-88. PMID: 15578930.
    Citations: 5     Fields:    
  44. Verma R, Peters NR, D'Onofrio M, Tochtrop GP, Sakamoto KM, Varadan R, Zhang M, Coffino P, Fushman D, Deshaies RJ, King RW. Ubistatins inhibit proteasome-dependent degradation by binding the ubiquitin chain. Science. 2004 Oct 01; 306(5693):117-20. PMID: 15459393.
    Citations: 57     Fields:    Translation:AnimalsCells
  45. Cheng D, Yadav N, King RW, Swanson MS, Weinstein EJ, Bedford MT. Small molecule regulators of protein arginine methyltransferases. J Biol Chem. 2004 Jun 04; 279(23):23892-9. PMID: 15056663.
    Citations: 74     Fields:    Translation:HumansCells
  46. Tochtrop G, King RW. Target identification strategies for chemical genetics. J. Comb. Chem. 2004; 7:677-688.
  47. King RW. Roughing up Smoothened: chemical modulators of hedgehog signaling. J Biol. 2002 Nov 06; 1(2):8. PMID: 12437769.
    Citations: 2     Fields:    Translation:HumansAnimalsCells
  48. Clemons PA, Koehler AN, Wagner BK, Sprigings TG, Spring DR, King RW, Schreiber SL, Foley MA. A one-bead, one-stock solution approach to chemical genetics: part 2. Chem Biol. 2001 Dec; 8(12):1183-95. PMID: 11755397.
    Citations: 13     Fields:    Translation:HumansCells
  49. Walling LA, Peters NR, Horn EJ, King RW. New technologies for chemical genetics. J Cell Biochem Suppl. 2001; Suppl 37:7-12. PMID: 11842422.
    Citations:    Fields:    
  50. Haggarty SJ, Mayer TU, Miyamoto DT, Fathi R, King RW, Mitchison TJ, Schreiber SL. Dissecting cellular processes using small molecules: identification of colchicine-like, taxol-like and other small molecules that perturb mitosis. Chem Biol. 2000 Apr; 7(4):275-86. PMID: 10780927.
    Citations: 26     Fields:    Translation:HumansAnimalsCells
  51. King RW. Chemistry or biology: which comes first after the genome is sequenced? Chem Biol. 1999 Dec; 6(12):R327-33. PMID: 10631509.
    Citations: 1     Fields:    
  52. Mayer TU, Kapoor TM, Haggarty SJ, King RW, Schreiber SL, Mitchison TJ. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science. 1999 Oct 29; 286(5441):971-4. PMID: 10542155.
    Citations: 371     Fields:    Translation:AnimalsCells
  53. Yu H, Peters JM, King RW, Page AM, Hieter P, Kirschner MW. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science. 1998 Feb 20; 279(5354):1219-22. PMID: 9469815.
    Citations: 74     Fields:    Translation:HumansAnimalsCells
  54. Peters JM, King RW, Deshaies RJ. Cell cycle control by ubiquitin-dependent proteolysis. Ubiquitin and the Biology of the Cell, Peters JM, Harris JR, and Finley D, Eds. 1998; 345-387.
  55. King RW, Lustig KD, Stukenberg PT, McGarry TJ, Kirschner MW. Expression cloning in the test tube. Science. 1997 Aug 15; 277(5328):973-4. PMID: 9281074.
    Citations: 12     Fields:    Translation:Cells
  56. Stukenberg PT, Lustig KD, McGarry TJ, King RW, Kuang J, Kirschner MW. Systematic identification of mitotic phosphoproteins. Curr Biol. 1997 May 01; 7(5):338-48. PMID: 9115395.
    Citations: 60     Fields:    Translation:AnimalsCells
  57. Lustig KD, Stukenberg PT, McGarry TJ, King RW, Cryns VL, Mead PE, Zon LI, Yuan J, Kirschner MW. Small pool expression screening: A novel strategy for the identification of genes involved in cell cycle control, apoptosis, and early development. Meth. Enzym. 1997; 283:83-99.
  58. Kirschner MW, King RW, Peters JM. Assay and reagents for detecting inhibitors of ubiquitin-dependent degradation of cell-cycle regulatory proteins. 1997.
  59. Mohrig JR, Rosenberg R, Apostol JW, Bastienaansen M, Evans JW, Franklin SJ, Frisbie CD, Fu SS, Hamm ML, Hirose CB, Hundstad DA, James TL, King RW, Larson CJ, Latham HA, Owen DA, Stein KA ,Warnet R. Diastereoselectivity of enolate anion protonation. H/D exchange of ƒÒ-substituted ethyl butanoates in ethanol-d. J. Amer. Chem. Soc. 1997; 119:479-486.
  60. King RW, Deshaies RJ, Peters JM, Kirschner MW. How proteolysis drives the cell cycle. Science. 1996 Dec 06; 274(5293):1652-9. PMID: 8939846.
    Citations: 318     Fields:    Translation:HumansAnimalsCells
  61. Peters JM, King RW, Höög C, Kirschner MW. Identification of BIME as a subunit of the anaphase-promoting complex. Science. 1996 Nov 15; 274(5290):1199-201. PMID: 8895470.
    Citations: 63     Fields:    Translation:AnimalsCells
  62. King RW, Glotzer M, Kirschner MW. Mutagenic analysis of the destruction signal of mitotic cyclins and structural characterization of ubiquitinated intermediates. Mol Biol Cell. 1996 Sep; 7(9):1343-57. PMID: 8885231.
    Citations: 103     Fields:    Translation:AnimalsCells
  63. Yu H, King RW, Peters JM, Kirschner MW. Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation. Curr Biol. 1996 Apr 01; 6(4):455-66. PMID: 8723350.
    Citations: 62     Fields:    Translation:HumansAnimalsCells
  64. King RW, Peters JM, Tugendreich S, Rolfe M, Hieter P, Kirschner MW. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. Cell. 1995 Apr 21; 81(2):279-88. PMID: 7736580.
    Citations: 306     Fields:    Translation:AnimalsCells
  65. King RW, Lustig KD, Stukenberg PT, Kirschner MW. Improved method of expression cloning. 1995.
  66. King, RW. Biochemical characterization of the cyclin B ubiquitin conjugation system. 1995.
  67. King RW, Jackson PK, Kirschner MW. Mitosis in transition. Cell. 1994 Nov 18; 79(4):563-71. PMID: 7954823.
    Citations: 161     Fields:    Translation:AnimalsCells
  68. Holloway SL, Glotzer M, King RW, Murray AW. Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor. Cell. 1993 Jul 02; 73(7):1393-402. PMID: 8391932.
    Citations: 180     Fields:    Translation:AnimalsCells
  69. Mohrig JR, Fu SS, King RW, Warnet RW, Gustafson G. Stereochemistry of nucleophilic conjugate addition. Addition of ethanol-d and 2-methyl-2-propanethiol-d to ethyl crotonate. J. Am. Chem. Soc. 1990; 112:3665-3667.
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.