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Sasha Anna Singh, Ph.D.

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Mentoring
Available: 11/16/20, Expires: 10/01/21

The Center for Interdisciplinary Cardiovascular Sciences (CICS) at Brigham and Women’s Hospital/Harvard Medical School is located in the Center for Life Sciences Boston at 3 Blackfan Street (https://cics.bwh.harvard.edu). CICS is at the forefront of cardiovascular disease research with more than 30 peer-reviewed publications in the last two years alone. We comprise a community of researchers with diverse clinical and basic research backgrounds including molecular biology, biochemistry, engineering, physics, and mathematics. A mainstay of CICS is the Proteomics Group headed by Dr. Sasha Singh. All research projects at CICS are supported by mass spectrometry-enabled proteomics and metabolomics. We are therefore looking for a student that is skilled at or developing skills for the computational sciences, such as bioinformatics, to join the Proteomics Group.

In particular, the candidate will provide computational support on a recently matured cardiovascular proteomics project. CICS is equipped with multiple bioinformatics software that will facilitate the candidate’s work. Dr. Singh will dedicate significant time to train and mentor the candidate through the project, but the candidate is also expected to develop an autonomous work ethic.

CICS is committed to providing a fulfilling research experience that also includes a safe and COVID-compliant environment. We therefore expect the candidate to work onsite as needed. A completely remote training period is not conducive to the project’s needs.


Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Lupieri A, Nagata Y, Passos LSA, Beker-Greene D, Kirkwood KA, Wylie-Sears J, Alvandi Z, Higashi H, Hung JW, Singh SA, Bischoff J, Levine RA, Aikawa E. Integration of Functional Imaging, Cytometry, and Unbiased Proteomics Reveals New Features of Endothelial-to-Mesenchymal Transition in Ischemic Mitral Valve Regurgitation in Human Patients. Front Cardiovasc Med. 2021; 8:688396. PMID: 34458332.
    Citations:    
  2. Hutcheson JD, Schlotter F, Creager MD, Li X, Pham T, Vyas P, Higashi H, Body SC, Aikawa M, Singh SA, Kos L, Aikawa E. Elastogenesis Correlates With Pigment Production in Murine Aortic Valve Leaflets. Front Cardiovasc Med. 2021; 8:678401. PMID: 34239903.
    Citations:    
  3. Decano JL, Singh SA, Gasparotto Bueno C, Ho Lee L, Halu A, Chelvanambi S, Matamalas JT, Zhang H, Mlynarchik AK, Qiao J, Sharma A, Mukai S, Wang J, Anderson DG, Ozaki CK, Libby P, Aikawa E, Aikawa M. Systems Approach to Discovery of Therapeutic Targets for Vein Graft Disease: PPARa Pivotally Regulates Metabolism, Activation, and Heterogeneity of Macrophages and Lesion Development. Circulation. 2021 Jun 22; 143(25):2454-2470. PMID: 33821665.
    Citations: 2     Fields:    
  4. Singh SA, Andraski AB, Higashi H, Lee LH, Ramsaroop A, Sacks FM, Aikawa M. Metabolism of PLTP, CETP, and LCAT on multiple HDL sizes using the Orbitrap Fusion Lumos. JCI Insight. 2021 02 08; 6(3). PMID: 33351780.
    Citations:    Fields:    Translation:Humans
  5. Rogers MA, Hutcheson JD, Okui T, Goettsch C, Singh SA, Halu A, Schlotter F, Higashi H, Wang L, Whelan MC, Mlynarchik AK, Daugherty A, Nomura M, Aikawa M, Aikawa E. Dynamin-related protein 1 inhibition reduces hepatic PCSK9 secretion. Cardiovasc Res. 2021 Feb 01. PMID: 33523181.
    Citations:    Fields:    
  6. Schlotter F, de Freitas RCC, Rogers MA, Blaser MC, Wu PJ, Higashi H, Halu A, Iqbal F, Andraski AB, Rodia CN, Kuraoka S, Wen JR, Creager M, Pham T, Hutcheson JD, Body SC, Kohan AB, Sacks FM, Aikawa M, Singh SA, Aikawa E. ApoC-III is a novel inducer of calcification in human aortic valves. J Biol Chem. 2021 Jan-Jun; 296:100193. PMID: 33334888.
    Citations: 2     Fields:    Translation:HumansCells
  7. Atkins SK, Singh SA, Aikawa E. Calcific Aortic Valve Disease "Omics" Is Timely, But Are We Looking Too Late? JACC Basic Transl Sci. 2020 Dec; 5(12):1178-1180. PMID: 33427821.
    Citations: 2     
  8. Okui T, Iwashita M, Rogers MA, Halu A, Atkins SK, Kuraoka S, Abdelhamid I, Higashi H, Ramsaroop A, Aikawa M, Singh SA, Aikawa E. CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing Factor in Vascular Calcification via Promoting Fatty Acid Metabolism and Mitochondrial Dysfunction. Arterioscler Thromb Vasc Biol. 2021 02; 41(2):755-768. PMID: 33356393.
    Citations: 1     Fields:    Translation:HumansAnimalsCells
  9. Rogers MA, Buffolo F, Schlotter F, Atkins SK, Lee LH, Halu A, Blaser MC, Tsolaki E, Higashi H, Luther K, Daaboul G, Bouten CVC, Body SC, Singh SA, Bertazzo S, Libby P, Aikawa M, Aikawa E. Annexin A1-dependent tethering promotes extracellular vesicle aggregation revealed with single-extracellular vesicle analysis. Sci Adv. 2020 Sep; 6(38). PMID: 32938681.
    Citations: 12     Fields:    
  10. Kawakami R, Katsuki S, Travers R, Romero DC, Becker-Greene D, Passos LSA, Higashi H, Blaser MC, Sukhova GK, Buttigieg J, Kopriva D, Schmidt AM, Anderson DG, Singh SA, Cardoso L, Weinbaum S, Libby P, Aikawa M, Croce K, Aikawa E. S100A9-RAGE Axis Accelerates Formation of Macrophage-Mediated Extracellular Vesicle Microcalcification in Diabetes Mellitus. Arterioscler Thromb Vasc Biol. 2020 08; 40(8):1838-1853. PMID: 32460581.
    Citations: 6     Fields:    Translation:HumansAnimalsCells
  11. Engelbrecht E, Levesque MV, He L, Vanlandewijck M, Nitzsche A, Niazi H, Kuo A, Singh SA, Aikawa M, Holton K, Proia RL, Kono M, Pu WT, Camerer E, Betsholtz C, Hla T. Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta. Elife. 2020 02 24; 9. PMID: 32091396.
    Citations: 7     Fields:    Translation:AnimalsCells
  12. Fehr AR, Singh SA, Kerr CM, Mukai S, Higashi H, Aikawa M. The impact of PARPs and ADP-ribosylation on inflammation and host-pathogen interactions. Genes Dev. 2020 03 01; 34(5-6):341-359. PMID: 32029454.
    Citations: 36     Fields:    Translation:HumansCells
  13. Arima N, Sasaki Y, Lee LH, Zhang H, Figueiredo JL, Mlynarchik AK, Qiao J, Yamada I, Higashi H, Ha AH, Halu A, Mizuno K, Singh SA, Yamazaki Y, Aikawa M. Multiorgan Systems Study Reveals Igfbp7 as a Suppressor of Gluconeogenesis after Gastric Bypass Surgery. J Proteome Res. 2020 01 03; 19(1):129-143. PMID: 31661273.
    Citations:    Fields:    Translation:HumansAnimalsCells
  14. Andraski AB, Singh SA, Lee LH, Higashi H, Smith N, Zhang B, Aikawa M, Sacks FM. Effects of Replacing Dietary Monounsaturated Fat With Carbohydrate on HDL (High-Density Lipoprotein) Protein Metabolism and Proteome Composition in Humans. Arterioscler Thromb Vasc Biol. 2019 11; 39(11):2411-2430. PMID: 31554421.
    Citations: 4     Fields:    Translation:HumansCTClinical Trials
  15. Goto S, Rogers MA, Blaser MC, Higashi H, Lee LH, Schlotter F, Body SC, Aikawa M, Singh SA, Aikawa E. Standardization of Human Calcific Aortic Valve Disease in vitro Modeling Reveals Passage-Dependent Calcification. Front Cardiovasc Med. 2019; 6:49. PMID: 31041314.
    Citations: 10     
  16. Higashi H, Maejima T, Lee LH, Yamazaki Y, Hottiger MO, Singh SA, Aikawa M. A Study into the ADP-Ribosylome of IFN-?-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation. J Proteome Res. 2019 04 05; 18(4):1607-1622. PMID: 30848916.
    Citations: 5     Fields:    Translation:HumansCells
  17. Nakano T, Katsuki S, Chen M, Decano JL, Halu A, Lee LH, Pestana DVS, Kum AST, Kuromoto RK, Golden WS, Boff MS, Guimaraes GC, Higashi H, Kauffman KJ, Maejima T, Suzuki T, Iwata H, Barabási AL, Aster JC, Anderson DG, Sharma A, Singh SA, Aikawa E, Aikawa M. Uremic Toxin Indoxyl Sulfate Promotes Proinflammatory Macrophage Activation Via the Interplay of OATP2B1 and Dll4-Notch Signaling. Circulation. 2019 01 02; 139(1):78-96. PMID: 30586693.
    Citations: 32     Fields:    Translation:HumansAnimalsCells
  18. Creager MD, Hohl T, Hutcheson JD, Moss AJ, Schlotter F, Blaser MC, Park MA, Lee LH, Singh SA, Alcaide-Corral CJ, Tavares AAS, Newby DE, Kijewski MF, Aikawa M, Di Carli M, Dweck MR, Aikawa E. 18F-Fluoride Signal Amplification Identifies Microcalcifications Associated With Atherosclerotic Plaque Instability in Positron Emission Tomography/Computed Tomography Images. Circ Cardiovasc Imaging. 2019 01; 12(1):e007835. PMID: 30642216.
    Citations: 28     Fields:    Translation:HumansAnimalsCells
  19. Lee LH, Halu A, Morgan S, Iwata H, Aikawa M, Singh SA. XINA: A Workflow for the Integration of Multiplexed Proteomics Kinetics Data with Network Analysis. J Proteome Res. 2019 02 01; 18(2):775-781. PMID: 30370770.
    Citations: 4     Fields:    Translation:HumansAnimalsCells
  20. Halu A, Wang JG, Iwata H, Mojcher A, Abib AL, Singh SA, Aikawa M, Sharma A. Context-enriched interactome powered by proteomics helps the identification of novel regulators of macrophage activation. Elife. 2018 10 10; 7. PMID: 30303482.
    Citations: 4     Fields:    Translation:HumansCells
  21. Schlotter F, Halu A, Goto S, Blaser MC, Body SC, Lee LH, Higashi H, DeLaughter DM, Hutcheson JD, Vyas P, Pham T, Rogers MA, Sharma A, Seidman CE, Loscalzo J, Seidman JG, Aikawa M, Singh SA, Aikawa E. Spatiotemporal Multi-Omics Mapping Generates a Molecular Atlas of the Aortic Valve and Reveals Networks Driving Disease. Circulation. 2018 07 24; 138(4):377-393. PMID: 29588317.
    Citations: 45     Fields:    Translation:HumansCells
  22. Lei Y. WITHDRAWN: Catalytically inactive phosphatase MTMR12 is a novel regulator of osteoclast function through F-actin ring formation. Biochim Biophys Acta Mol Basis Dis. 2018 Jun 12. PMID: 29902551.
    Citations:    Fields:    
  23. Singh SA, Aikawa M. Unbiased and targeted mass spectrometry for the HDL proteome. Curr Opin Lipidol. 2017 02; 28(1):68-77. PMID: 28002079.
    Citations: 7     Fields:    Translation:HumansAnimals
  24. Lee LH, Andraski AB, Pieper B, Higashi H, Sacks FM, Aikawa M, Singh SA. Automation of PRM-dependent D3-Leu tracer enrichment in HDL to study the metabolism of apoA-I, LCAT and other apolipoproteins. Proteomics. 2017 01; 17(1-2). PMID: 27862954.
    Citations: 2     Fields:    Translation:Humans
  25. Iwata H, Goettsch C, Sharma A, Ricchiuto P, Goh WW, Halu A, Yamada I, Yoshida H, Hara T, Wei M, Inoue N, Fukuda D, Mojcher A, Mattson PC, Barabási AL, Boothby M, Aikawa E, Singh SA, Aikawa M. PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation. Nat Commun. 2016 10 31; 7:12849. PMID: 27796300.
    Citations: 60     Fields:    Translation:HumansAnimalsCells
  26. Singh SA, Aikawa E, Aikawa M. Current Trends and Future Perspectives of State-of-the-Art Proteomics Technologies Applied to Cardiovascular Disease Research. Circ J. 2016 Jul 25; 80(8):1674-83. PMID: 27430298.
    Citations: 3     Fields:    Translation:HumansAnimals
  27. Goettsch C, Hutcheson JD, Aikawa M, Iwata H, Pham T, Nykjaer A, Kjolby M, Rogers M, Michel T, Shibasaki M, Hagita S, Kramann R, Rader DJ, Libby P, Singh SA, Aikawa E. Sortilin mediates vascular calcification via its recruitment into extracellular vesicles. J Clin Invest. 2016 Apr 01; 126(4):1323-36. PMID: 26950419.
    Citations: 63     Fields:    Translation:HumansAnimalsCells
  28. Singh SA, Andraski AB, Pieper B, Goh W, Mendivil CO, Sacks FM, Aikawa M. Multiple apolipoprotein kinetics measured in human HDL by high-resolution/accurate mass parallel reaction monitoring. J Lipid Res. 2016 Apr; 57(4):714-28. PMID: 26862155.
    Citations: 15     Fields:    Translation:HumansCells
  29. Singh SA, Miyosawa K, Aikawa M. Mass spectrometry meets the challenge of understanding the complexity of the lipoproteome: recent findings regarding proteins involved in dyslipidemia and cardiovascular disease. Expert Rev Proteomics. 2015; 12(5):519-32. PMID: 26325144.
    Citations: 3     Fields:    Translation:HumansAnimals
  30. Ricchiuto P, Iwata H, Yabusaki K, Yamada I, Pieper B, Sharma A, Aikawa M, Singh SA. mIMT-visHTS: A novel method for multiplexing isobaric mass tagged datasets with an accompanying visualization high throughput screening tool for protein profiling. J Proteomics. 2015 Oct 14; 128:132-40. PMID: 26232111.
    Citations: 3     Fields:    Translation:AnimalsCells
  31. Miyosawa K, Watanabe Y, Murakami K, Murakami T, Shibata H, Iwashita M, Yamazaki H, Yamazaki K, Ohgiya T, Shibuya K, Mizuno K, Tanabe S, Singh SA, Aikawa M. New CETP inhibitor K-312 reduces PCSK9 expression: a potential effect on LDL cholesterol metabolism. Am J Physiol Endocrinol Metab. 2015 Jul 15; 309(2):E177-90. PMID: 26015437.
    Citations: 12     Fields:    Translation:HumansAnimalsCells
  32. Hutcheson JD, Goettsch C, Pham T, Iwashita M, Aikawa M, Singh SA, Aikawa E. Enrichment of calcifying extracellular vesicles using density-based ultracentrifugation protocol. J Extracell Vesicles. 2014; 3:25129. PMID: 25491249.
    Citations: 19     
  33. Singh SA, Winter D, Kirchner M, Chauhan R, Ahmed S, Ozlu N, Tzur A, Steen JA, Steen H. Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation. EMBO J. 2014 Feb 18; 33(4):385-99. PMID: 24510915.
    Citations: 29     Fields:    Translation:HumansCells
  34. Singh SA, Winter D, Bilimoria PM, Bonni A, Steen H, Steen JA. FLEXIQinase, a mass spectrometry-based assay, to unveil multikinase mechanisms. Nat Methods. 2012 Apr 08; 9(5):504-8. PMID: 22484849.
    Citations: 12     Fields:    Translation:Cells
  35. Singh S, Springer M, Steen J, Kirschner MW, Steen H. FLEXIQuant: a novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J Proteome Res. 2009 May; 8(5):2201-10. PMID: 19344176.
    Citations: 36     Fields:    Translation:HumansCells
  36. Wang Z, Dong G, Singh S, Steen H, Li J. A simple and effective method for detecting phosphopeptides for phosphoproteomic analysis. J Proteomics. 2009 Jul 21; 72(5):831-5. PMID: 19341826.
    Citations: 5     Fields:    Translation:AnimalsCells
  37. Singh S, Stavrinides J, Christendat D, Guttman DS. A phylogenomic analysis of the shikimate dehydrogenases reveals broadscale functional diversification and identifies one functionally distinct subclass. Mol Biol Evol. 2008 Oct; 25(10):2221-32. PMID: 18669580.
    Citations: 3     Fields:    Translation:Cells
  38. Singh SA, Christendat D. Structure of Arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase and implications for metabolic channeling in the shikimate pathway. Biochemistry. 2006 Jun 27; 45(25):7787-96. PMID: 16784230.
    Citations: 22     Fields:    Translation:AnimalsCells
  39. Bonvin J, Aponte RA, Marcantonio M, Singh S, Christendat D, Turnbull JL. Biochemical characterization of prephenate dehydrogenase from the hyperthermophilic bacterium Aquifex aeolicus. Protein Sci. 2006 Jun; 15(6):1417-32. PMID: 16731976.
    Citations: 8     Fields:    Translation:Cells
  40. Sun W, Singh S, Zhang R, Turnbull JL, Christendat D. Crystal structure of prephenate dehydrogenase from Aquifex aeolicus. Insights into the catalytic mechanism. J Biol Chem. 2006 May 05; 281(18):12919-28. PMID: 16513644.
    Citations: 9     Fields:    Translation:Cells
  41. Singh S, Korolev S, Koroleva O, Zarembinski T, Collart F, Joachimiak A, Christendat D. Crystal structure of a novel shikimate dehydrogenase from Haemophilus influenzae. J Biol Chem. 2005 Apr 29; 280(17):17101-8. PMID: 15735308.
    Citations: 11     Fields:    Translation:Cells
  42. Hardtke CS, Ckurshumova W, Vidaurre DP, Singh SA, Stamatiou G, Tiwari SB, Hagen G, Guilfoyle TJ, Berleth T. Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4. Development. 2004 Mar; 131(5):1089-100. PMID: 14973283.
    Citations: 110     Fields:    Translation:AnimalsCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.