Role Description: While there have been exciting advances in the treatment of CF in the past few years, chronic lung infections remain a vexing problem that make up the one of the most difficult aspects of care for patients. Vaccines to prevent the infections, particularly for the organism known as Pseudomonas aeruginosa, could greatly improve overall health and protect against lung damage and respiratory problems. However, discovering vaccines is an inexact science, with most of the work done to date following an empiric process with a lot of trial and error, and so, far, in regard to P. aeruginosa, no success. Our research proposal will use the tools of modern biology and genetics to try to expedite the process of discovering antigens by allowing the immune system of an immunized animal to determine which components of the microbe make the best target antigens. After using techniques of rapid and high-level sequencing of DNA and immunology, specific candidate vaccines will be prepared and used to immunize mice to see if the candidate does, indeed, elicit an immune response that protects against lung infection. With the candidates identified it will then be possible to further develop them for human testing, determine if CF patients lack immunity to the vaccine, thus explaining their susceptibility to infection, with the ultimate goal to alter the course of lung disease via successful vaccination.