Contact, publication, and social network information about Harvard faculty and fellows. Harvard Catalyst Profiles
Check out the new look and feel of Harvard Catalyst Profiles! Learn more on our About and Help pages.

Pamela A. Silver, Ph.D.

Title
Institution
Department
Address
Phone
Fax

Mentoring
current student opportunities
Available: 07/06/17, Expires: 07/05/19

We have general capabilities in construction and testing of targeted fusion proteins and also bacteria and mammalian cells engineered with complex genetic circuits. We seek students with interest in a particular disease that they want to treat. We currently are developing treatments for anemia, cancer, certain viruses and other diseases and disorders.


Research
research activities and funding
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
Most Recent  |  List All
  1. T32GM080177 (MITCHISON, TIMOTHY J) Jul 1, 2010 - Jun 30, 2020
    NIH/NIGMS
    Harvard Systems Biology Graduate Program
    Role: Co-Principal Investigator
  2. R01GM057476 (SILVER, PAMELA A.) May 1, 1998 - Apr 30, 2011
    NIH/NIGMS
    Connecting the Genome to the Cytoplasm
    Role: Principal Investigator
  3. R37GM036373 (SILVER, PAMELA A) Jun 1, 1993 - Mar 31, 2013
    NIH/NIGMS
    Transport of Proteins In and Out of the Nucleus
    Role: Principal Investigator
  4. R01GM047385 (SILVER, PAMELA A.) May 1, 1992 - May 31, 1997
    NIH/NIGMS
    EUKARYOTIC DNA J PROTEINS THAT REGULATE HSP70 ACTION
    Role: Principal Investigator
  5. R01GM036373 (SILVER, PAMELA A) Apr 1, 1986 - Mar 31, 2017
    NIH/NIGMS
    Designing Biological Circuits
    Role: Principal Investigator

Bibliographic
selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Spady ES, Wyche TP, Rollins NJ, Clardy J, Way JC, Silver PA. Mammalian Cells Engineered to Produce Novel Steroids. Chembiochem. 2018 Jun 21. PMID: 29931794.
    View in: PubMed
  2. Ziesack M, Rollins N, Shah A, Dusel B, Webster G, Silver PA, Way JC. Chimeric Fatty Acyl-Acyl Carrier Protein Thioesterases Provide Mechanistic Insight into Enzyme Specificity and Expression. Appl Environ Microbiol. 2018 May 15; 84(10). PMID: 29549102.
    View in: PubMed
  3. Lau YH, Giessen TW, Altenburg WJ, Silver PA. Prokaryotic nanocompartments form synthetic organelles in a eukaryote. Nat Commun. 2018 Apr 03; 9(1):1311. PMID: 29615617.
    View in: PubMed
  4. Riglar DT, Silver PA. Engineering bacteria for diagnostic and therapeutic applications. Nat Rev Microbiol. 2018 Apr; 16(4):214-225. PMID: 29398705.
    View in: PubMed
  5. Stirling F, Bitzan L, O'Keefe S, Redfield E, Oliver JWK, Way J, Silver PA. Rational Design of Evolutionarily Stable Microbial Kill Switches. Mol Cell. 2017 Nov 16; 68(4):686-697.e3. PMID: 29149596.
    View in: PubMed
  6. Nangle SN, Sakimoto KK, Silver PA, Nocera DG. Biological-inorganic hybrid systems as a generalized platform for chemical production. Curr Opin Chem Biol. 2017 Dec; 41:107-113. PMID: 29136557.
    View in: PubMed
  7. Kuo J, Stirling F, Lau YH, Shulgina Y, Way JC, Silver PA. Synthetic genome recoding: new genetic codes for new features. Curr Genet. 2018 Apr; 64(2):327-333. PMID: 28983660.
    View in: PubMed
  8. Niederhuber MJ, Lambert TJ, Yapp C, Silver PA, Polka JK. Superresolution microscopy of the ß-carboxysome reveals a homogeneous matrix. Mol Biol Cell. 2017 Oct 01; 28(20):2734-2745. PMID: 28963440.
    View in: PubMed
  9. Niederhuber MJ, Lambert TJ, Yapp C, Silver PA, Polka JK. Super-resolution microscopy of the ß-carboxysome reveals a homogenous matrix. Mol Biol Cell. 2017 Aug 09. PMID: 28794262.
    View in: PubMed
  10. Green AA, Kim J, Ma D, Silver PA, Collins JJ, Yin P. Complex cellular logic computation using ribocomputing devices. Nature. 2017 08 03; 548(7665):117-121. PMID: 28746304.
    View in: PubMed
  11. Lau YH, Stirling F, Kuo J, Karrenbelt MAP, Chan YA, Riesselman A, Horton CA, Schäfer E, Lips D, Weinstock MT, Gibson DG, Way JC, Silver PA. Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA. Nucleic Acids Res. 2017 Jun 20; 45(11):6971-6980. PMID: 28499033.
    View in: PubMed
  12. Liu C, Sakimoto KK, Colón BC, Silver PA, Nocera DG. Ambient nitrogen reduction cycle using a hybrid inorganic-biological system. Proc Natl Acad Sci U S A. 2017 06 20; 114(25):6450-6455. PMID: 28588143.
    View in: PubMed
  13. Liu C, Nangle SN, Colón BC, Silver PA, Nocera DG. 13C-Labeling the carbon-fixation pathway of a highly efficient artificial photosynthetic system. Faraday Discuss. 2017 Jun 02; 198:529-537. PMID: 28294218.
    View in: PubMed
  14. Riglar DT, Giessen TW, Baym M, Kerns SJ, Niederhuber MJ, Bronson RT, Kotula JW, Gerber GK, Way JC, Silver PA. Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation. Nat Biotechnol. 2017 Jul; 35(7):653-658. PMID: 28553941.
    View in: PubMed
  15. Brown DM, Chan YA, Desai PJ, Grzesik P, Oldfield LM, Vashee S, Way JC, Silver PA, Glass JI. Efficient size-independent chromosome delivery from yeast to cultured cell lines. Nucleic Acids Res. 2017 Apr 20; 45(7):e50. PMID: 27980064.
    View in: PubMed
  16. Giessen TW, Silver PA. Widespread distribution of encapsulin nanocompartments reveals functional diversity. Nat Microbiol. 2017 Mar 06; 2:17029. PMID: 28263314.
    View in: PubMed
  17. Hays SG, Yan LLW, Silver PA, Ducat DC. Synthetic photosynthetic consortia define interactions leading to robustness and photoproduction. J Biol Eng. 2017; 11:4. PMID: 28127397.
    View in: PubMed
  18. Giessen TW, Silver PA. Engineering carbon fixation with artificial protein organelles. Curr Opin Biotechnol. 2017 08; 46:42-50. PMID: 28126670.
    View in: PubMed
  19. Liu X, Lopez PA, Giessen TW, Giles M, Way JC, Silver PA. Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution. Sci Rep. 2016 11 29; 6:38019. PMID: 27897245.
    View in: PubMed
  20. Giessen TW, Silver PA. A Catalytic Nanoreactor Based on in Vivo Encapsulation of Multiple Enzymes in an Engineered Protein Nanocompartment. Chembiochem. 2016 10 17; 17(20):1931-1935. PMID: 27504846.
    View in: PubMed
  21. Myhrvold C, Polka JK, Silver PA. Synthetic Lipid-Containing Scaffolds Enhance Production by Colocalizing Enzymes. ACS Synth Biol. 2016 12 16; 5(12):1396-1403. PMID: 27487319.
    View in: PubMed
  22. Polka JK, Hays SG, Silver PA. Building Spatial Synthetic Biology with Compartments, Scaffolds, and Communities. Cold Spring Harb Perspect Biol. 2016 Aug 01; 8(8). PMID: 27270297.
    View in: PubMed
  23. Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA, Del Vecchio D, Collins JJ. Creating Single-Copy Genetic Circuits. Mol Cell. 2016 07 21; 63(2):329-336. PMID: 27425413.
    View in: PubMed
  24. Giessen TW, Silver PA. Converting a Natural Protein Compartment into a Nanofactory for the Size-Constrained Synthesis of Antimicrobial Silver Nanoparticles. ACS Synth Biol. 2016 12 16; 5(12):1497-1504. PMID: 27276075.
    View in: PubMed
  25. Liu C, Colón BC, Ziesack M, Silver PA, Nocera DG. Water splitting-biosynthetic system with CO2 reduction efficiencies exceeding photosynthesis. Science. 2016 Jun 03; 352(6290):1210-3. PMID: 27257255.
    View in: PubMed
  26. Boeke JD, Church G, Hessel A, Kelley NJ, Arkin A, Cai Y, Carlson R, Chakravarti A, Cornish VW, Holt L, Isaacs FJ, Kuiken T, Lajoie M, Lessor T, Lunshof J, Maurano MT, Mitchell LA, Rine J, Rosser S, Sanjana NE, Silver PA, Valle D, Wang H, Way JC, Yang L. GENOME ENGINEERING. The Genome Project-Write. Science. 2016 Jul 08; 353(6295):126-7. PMID: 27256881.
    View in: PubMed
  27. Burrill DR, Vernet A, Collins JJ, Silver PA, Way JC. Targeted erythropoietin selectively stimulates red blood cell expansion in vivo. Proc Natl Acad Sci U S A. 2016 May 10; 113(19):5245-50. PMID: 27114509; PMCID: PMC4868492 [Available on 11/10/16].
    View in: PubMed, PubMed Central
  28. Polka JK, Silver PA. A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth Biol. 2016 Apr 15; 5(4):303-11. PMID: 26814170.
    View in: PubMed
  29. Liu X, Hicks WM, Silver PA, Way JC. Engineering acyl carrier protein to enhance production of shortened fatty acids. Biotechnol Biofuels. 2016; 9:24. PMID: 26839587; PMCID: PMC4736557.
    View in: PubMed, PubMed Central
  30. MacKellar D, Lieber L, Norman JS, Bolger A, Tobin C, Murray JW, Oksaksin M, Chang RL, Ford TJ, Nguyen PQ, Woodward J, Permingeat HR, Joshi NS, Silver PA, Usadel B, Rutherford AW, Friesen ML, Prell J. Streptomyces thermoautotrophicus does not fix nitrogen. Sci Rep. 2016 Feb 01; 6:20086. PMID: 26833023; PMCID: PMC4735515.
    View in: PubMed, PubMed Central
  31. Biteen JS, Blainey PC, Cardon ZG, Chun M, Church GM, Dorrestein PC, Fraser SE, Gilbert JA, Jansson JK, Knight R, Miller JF, Ozcan A, Prather KA, Quake SR, Ruby EG, Silver PA, Taha S, van den Engh G, Weiss PS, Wong GC, Wright AT, Young TD. Tools for the Microbiome: Nano and Beyond. ACS Nano. 2016 Jan 26; 10(1):6-37. PMID: 26695070.
    View in: PubMed
  32. Myhrvold C, Kotula JW, Hicks WM, Conway NJ, Silver PA. A distributed cell division counter reveals growth dynamics in the gut microbiota. Nat Commun. 2015 Nov 30; 6:10039. PMID: 26615910; PMCID: PMC4674677.
    View in: PubMed, PubMed Central
  33. Giessen TW, Silver PA. Encapsulation as a Strategy for the Design of Biological Compartmentalization. J Mol Biol. 2016 Feb 27; 428(5 Pt B):916-27. PMID: 26403362.
    View in: PubMed
  34. Hays SG, Patrick WG, Ziesack M, Oxman N, Silver PA. Better together: engineering and application of microbial symbioses. Curr Opin Biotechnol. 2015 Dec; 36:40-9. PMID: 26319893.
    View in: PubMed
  35. Ford TJ, Silver PA. Synthetic biology expands chemical control of microorganisms. Curr Opin Chem Biol. 2015 Oct; 28:20-8. PMID: 26056951; PMCID: PMC4961477.
    View in: PubMed, PubMed Central
  36. Chang ZL, Silver PA, Chen YY. Identification and selective expansion of functionally superior T cells expressing chimeric antigen receptors. J Transl Med. 2015 May 20; 13:161. PMID: 25990251; PMCID: PMC4457995.
    View in: PubMed, PubMed Central
  37. Torella JP, Gagliardi CJ, Chen JS, Bediako DK, Colón B, Way JC, Silver PA, Nocera DG. Efficient solar-to-fuels production from a hybrid microbial-water-splitting catalyst system. Proc Natl Acad Sci U S A. 2015 Feb 24; 112(8):2337-42. PMID: 25675518; PMCID: PMC4345567.
    View in: PubMed, PubMed Central
  38. Myhrvold C, Silver PA. Using synthetic RNAs as scaffolds and regulators. Nat Struct Mol Biol. 2015 Jan; 22(1):8-10. PMID: 25565027.
    View in: PubMed
  39. Chen AH, Lubkowicz D, Yeong V, Chang RL, Silver PA. Transplantability of a circadian clock to a noncircadian organism. Sci Adv. 2015; 1(5). PMID: 26229984.
    View in: PubMed
  40. Robinson-Mosher A, Chen JH, Way J, Silver PA. Designing cell-targeted therapeutic proteins reveals the interplay between domain connectivity and cell binding. Biophys J. 2014 Nov 18; 107(10):2456-66. PMID: 25418314; PMCID: PMC4241446.
    View in: PubMed, PubMed Central
  41. Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell. 2014 Nov 06; 159(4):925-39. PMID: 25417166; PMCID: PMC4265554.
    View in: PubMed, PubMed Central
  42. Polka JK, Silver PA. Induced sensitivity of Bacillus subtilis colony morphology to mechanical media compression. PeerJ. 2014; 2:e597. PMID: 25289183; PMCID: PMC4183962.
    View in: PubMed, PubMed Central
  43. Torella JP, Lienert F, Boehm CR, Chen JH, Way JC, Silver PA. Unique nucleotide sequence-guided assembly of repetitive DNA parts for synthetic biology applications. Nat Protoc. 2014 Sep; 9(9):2075-89. PMID: 25101822; PMCID: PMC4899833.
    View in: PubMed, PubMed Central
  44. Sachdeva G, Garg A, Godding D, Way JC, Silver PA. In vivo co-localization of enzymes on RNA scaffolds increases metabolic production in a geometrically dependent manner. Nucleic Acids Res. 2014 Aug; 42(14):9493-503. PMID: 25034694; PMCID: PMC4132732.
    View in: PubMed, PubMed Central
  45. Silver PA, Way JC, Arnold FH, Meyerowitz JT. Synthetic biology: Engineering explored. Nature. 2014 May 08; 509(7499):166-7. PMID: 24805338.
    View in: PubMed
  46. Mattozzi MD, Voges MJ, Silver PA, Way JC. Transient gene expression in tobacco using Gibson assembly and the Gene Gun. J Vis Exp. 2014 Apr 18; (86). PMID: 24796418; PMCID: PMC4172236.
    View in: PubMed, PubMed Central
  47. Way JC, Collins JJ, Keasling JD, Silver PA. Integrating biological redesign: where synthetic biology came from and where it needs to go. Cell. 2014 Mar 27; 157(1):151-61. PMID: 24679533.
    View in: PubMed
  48. Kotula JW, Kerns SJ, Shaket LA, Siraj L, Collins JJ, Way JC, Silver PA. Programmable bacteria detect and record an environmental signal in the mammalian gut. Proc Natl Acad Sci U S A. 2014 Apr 01; 111(13):4838-43. PMID: 24639514; PMCID: PMC3977281.
    View in: PubMed, PubMed Central
  49. Weyn-Vanhentenryck SM, Mele A, Yan Q, Sun S, Farny N, Zhang Z, Xue C, Herre M, Silver PA, Zhang MQ, Krainer AR, Darnell RB, Zhang C. HITS-CLIP and integrative modeling define the Rbfox splicing-regulatory network linked to brain development and autism. Cell Rep. 2014 Mar 27; 6(6):1139-1152. PMID: 24613350; PMCID: PMC3992522.
    View in: PubMed, PubMed Central
  50. Lienert F, Lohmueller JJ, Garg A, Silver PA. Synthetic biology in mammalian cells: next generation research tools and therapeutics. Nat Rev Mol Cell Biol. 2014 Feb; 15(2):95-107. PMID: 24434884; PMCID: PMC4032074.
    View in: PubMed, PubMed Central
  51. Polka JK, Silver PA. Building synthetic cellular organization. Mol Biol Cell. 2013 Dec; 24(23):3585-7. PMID: 24288075; PMCID: PMC3842987.
    View in: PubMed, PubMed Central
  52. Wintermute EH, Lieberman TD, Silver PA. An objective function exploiting suboptimal solutions in metabolic networks. BMC Syst Biol. 2013 Oct 03; 7:98. PMID: 24088221; PMCID: PMC4016239.
    View in: PubMed, PubMed Central
  53. Torella JP, Boehm CR, Lienert F, Chen JH, Way JC, Silver PA. Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic Acids Res. 2014 Jan; 42(1):681-9. PMID: 24078086; PMCID: PMC3874176.
    View in: PubMed, PubMed Central
  54. Inniss MC, Silver PA. Building synthetic memory. Curr Biol. 2013 Sep 09; 23(17):R812-6. PMID: 24028965; PMCID: PMC3821973.
    View in: PubMed, PubMed Central
  55. Voges MJ, Silver PA, Way JC, Mattozzi MD. Targeting a heterologous protein to multiple plant organelles via rationally designed 5' mRNA tags. J Biol Eng. 2013 Sep 08; 7(1):20. PMID: 24011257; PMCID: PMC3847293.
    View in: PubMed, PubMed Central
  56. Chen AH, Robinson-Mosher A, Savage DF, Silver PA, Polka JK. The bacterial carbon-fixing organelle is formed by shell envelopment of preassembled cargo. PLoS One. 2013; 8(9):e76127. PMID: 24023971; PMCID: PMC3762834.
    View in: PubMed, PubMed Central
  57. Lienert F, Torella JP, Chen JH, Norsworthy M, Richardson RR, Silver PA. Two- and three-input TALE-based AND logic computation in embryonic stem cells. Nucleic Acids Res. 2013 Nov; 41(21):9967-75. PMID: 23982518; PMCID: PMC3834826.
    View in: PubMed, PubMed Central
  58. Myhrvold C, Dai M, Silver PA, Yin P. Isothermal self-assembly of complex DNA structures under diverse and biocompatible conditions. Nano Lett. 2013 Sep 11; 13(9):4242-8. PMID: 23978049.
    View in: PubMed
  59. Torella JP, Ford TJ, Kim SN, Chen AM, Way JC, Silver PA. Tailored fatty acid synthesis via dynamic control of fatty acid elongation. Proc Natl Acad Sci U S A. 2013 Jul 09; 110(28):11290-5. PMID: 23798438; PMCID: PMC3710846.
    View in: PubMed, PubMed Central
  60. Robinson-Mosher A, Shinar T, Silver PA, Way J. Dynamics simulations for engineering macromolecular interactions. Chaos. 2013 Jun; 23(2):025110. PMID: 23822508; PMCID: PMC3695996.
    View in: PubMed, PubMed Central
  61. Selgrade DF, Lohmueller JJ, Lienert F, Silver PA. Protein scaffold-activated protein trans-splicing in mammalian cells. J Am Chem Soc. 2013 May 22; 135(20):7713-9. PMID: 23621664; PMCID: PMC4006946.
    View in: PubMed, PubMed Central
  62. Wang Q, Moore MJ, Adelmant G, Marto JA, Silver PA. PQBP1, a factor linked to intellectual disability, affects alternative splicing associated with neurite outgrowth. Genes Dev. 2013 Mar 15; 27(6):615-26. PMID: 23512658; PMCID: PMC3613609.
    View in: PubMed, PubMed Central
  63. Mattozzi Md, Ziesack M, Voges MJ, Silver PA, Way JC. Expression of the sub-pathways of the Chloroflexus aurantiacus 3-hydroxypropionate carbon fixation bicycle in E. coli: Toward horizontal transfer of autotrophic growth. Metab Eng. 2013 Mar; 16:130-9. PMID: 23376595.
    View in: PubMed
  64. Chen AH, Afonso B, Silver PA, Savage DF. Spatial and temporal organization of chromosome duplication and segregation in the cyanobacterium Synechococcus elongatus PCC 7942. PLoS One. 2012; 7(10):e47837. PMID: 23112856; PMCID: PMC3480399.
    View in: PubMed, PubMed Central
  65. Delebecque CJ, Silver PA, Lindner AB. Designing and using RNA scaffolds to assemble proteins in vivo. Nat Protoc. 2012 Oct; 7(10):1797-807. PMID: 22955695.
    View in: PubMed
  66. Chen AH, Silver PA. Designing biological compartmentalization. Trends Cell Biol. 2012 Dec; 22(12):662-70. PMID: 22841504.
    View in: PubMed
  67. Chen AJ, Paik JH, Zhang H, Shukla SA, Mortensen R, Hu J, Ying H, Hu B, Hurt J, Farny N, Dong C, Xiao Y, Wang YA, Silver PA, Chin L, Vasudevan S, Depinho RA. STAR RNA-binding protein Quaking suppresses cancer via stabilization of specific miRNA. Genes Dev. 2012 Jul 01; 26(13):1459-72. PMID: 22751500; PMCID: PMC3403014.
    View in: PubMed, PubMed Central
  68. Burrill DR, Inniss MC, Boyle PM, Silver PA. Synthetic memory circuits for tracking human cell fate. Genes Dev. 2012 Jul 01; 26(13):1486-97. PMID: 22751502; PMCID: PMC3403016.
    View in: PubMed, PubMed Central
  69. Boyle PM, Burrill DR, Inniss MC, Agapakis CM, Deardon A, DeWerd JG, Gedeon MA, Quinn JY, Paull ML, Raman AM, Theilmann MR, Wang L, Winn JC, Medvedik O, Schellenberg K, Haynes KA, Viel A, Brenner TJ, Church GM, Shah JV, Silver PA. A BioBrick compatible strategy for genetic modification of plants. J Biol Eng. 2012 Jun 20; 6(1):8. PMID: 22716313; PMCID: PMC3537565.
    View in: PubMed, PubMed Central
  70. Ducat DC, Silver PA. Improving carbon fixation pathways. Curr Opin Chem Biol. 2012 Aug; 16(3-4):337-44. PMID: 22647231; PMCID: PMC3424341.
    View in: PubMed, PubMed Central
  71. Agapakis CM, Boyle PM, Silver PA. Natural strategies for the spatial optimization of metabolism in synthetic biology. Nat Chem Biol. 2012 May 17; 8(6):527-35. PMID: 22596204.
    View in: PubMed
  72. Garg A, Lohmueller JJ, Silver PA, Armel TZ. Engineering synthetic TAL effectors with orthogonal target sites. Nucleic Acids Res. 2012 Aug; 40(15):7584-95. PMID: 22581776; PMCID: PMC3424557.
    View in: PubMed, PubMed Central
  73. Adelmant G, Calkins AS, Garg BK, Card JD, Askenazi M, Miron A, Sobhian B, Zhang Y, Nakatani Y, Silver PA, Iglehart JD, Marto JA, Lazaro JB. DNA ends alter the molecular composition and localization of Ku multicomponent complexes. Mol Cell Proteomics. 2012 Aug; 11(8):411-21. PMID: 22535209; PMCID: PMC3412971.
    View in: PubMed, PubMed Central
  74. Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. A sensitive switch for visualizing natural gene silencing in single cells. ACS Synth Biol. 2012 Mar 16; 1(3):99-106. PMID: 22530199; PMCID: PMC3331714.
    View in: PubMed, PubMed Central
  75. Nishida K, Silver PA. Induction of biogenic magnetization and redox control by a component of the target of rapamycin complex 1 signaling pathway. PLoS Biol. 2012; 10(2):e1001269. PMID: 22389629; PMCID: PMC3289596.
    View in: PubMed, PubMed Central
  76. Lohmueller JJ, Armel TZ, Silver PA. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Res. 2012 Jun; 40(11):5180-7. PMID: 22323524; PMCID: PMC3367183.
    View in: PubMed, PubMed Central
  77. Silver P. Pamela Silver: synthesizing a new biology. Interview by Caitlin Sedwick. J Cell Biol. 2012 Feb 06; 196(3):302-3. PMID: 22312001; PMCID: PMC3275369.
    View in: PubMed, PubMed Central
  78. Ducat DC, Avelar-Rivas JA, Way JC, Silver PA. Rerouting carbon flux to enhance photosynthetic productivity. Appl Environ Microbiol. 2012 Apr; 78(8):2660-8. PMID: 22307292; PMCID: PMC3318813.
    View in: PubMed, PubMed Central
  79. Bonacci W, Teng PK, Afonso B, Niederholtmeyer H, Grob P, Silver PA, Savage DF. Modularity of a carbon-fixing protein organelle. Proc Natl Acad Sci U S A. 2012 Jan 10; 109(2):478-83. PMID: 22184212; PMCID: PMC3258634.
    View in: PubMed, PubMed Central
  80. Burrill DR, Boyle PM, Silver PA. A new approach to an old problem: synthetic biology tools for human disease and metabolism. Cold Spring Harb Symp Quant Biol. 2011; 76:145-54. PMID: 22169233.
    View in: PubMed
  81. Boyle PM, Silver PA. Parts plus pipes: synthetic biology approaches to metabolic engineering. Metab Eng. 2012 May; 14(3):223-32. PMID: 22037345; PMCID: PMC3293987.
    View in: PubMed, PubMed Central
  82. Burrill DR, Silver PA. Recording cellular experiences of DNA damage. Cell Cycle. 2011 Aug 01; 10(15):2410-1. PMID: 21670599.
    View in: PubMed
  83. Waks Z, Klein AM, Silver PA. Cell-to-cell variability of alternative RNA splicing. Mol Syst Biol. 2011 Jul 05; 7:506. PMID: 21734645; PMCID: PMC3159976.
    View in: PubMed, PubMed Central
  84. Delebecque CJ, Lindner AB, Silver PA, Aldaye FA. Organization of intracellular reactions with rationally designed RNA assemblies. Science. 2011 Jul 22; 333(6041):470-4. PMID: 21700839.
    View in: PubMed
  85. Haynes KA, Silver PA. Synthetic reversal of epigenetic silencing. J Biol Chem. 2011 Aug 05; 286(31):27176-82. PMID: 21669865; PMCID: PMC3149311.
    View in: PubMed, PubMed Central
  86. Shih JD, Waks Z, Kedersha N, Silver PA. Visualization of single mRNAs reveals temporal association of proteins with microRNA-regulated mRNA. Nucleic Acids Res. 2011 Sep 01; 39(17):7740-9. PMID: 21653551; PMCID: PMC3177202.
    View in: PubMed, PubMed Central
  87. Barstow B, Agapakis CM, Boyle PM, Grandl G, Silver PA, Wintermute EH. A synthetic system links FeFe-hydrogenases to essential E. coli sulfur metabolism. J Biol Eng. 2011 May 26; 5:7. PMID: 21615937; PMCID: PMC3130634.
    View in: PubMed, PubMed Central
  88. Agapakis CM, Niederholtmeyer H, Noche RR, Lieberman TD, Megason SG, Way JC, Silver PA. Towards a synthetic chloroplast. PLoS One. 2011 Apr 20; 6(4):e18877. PMID: 21533097.
    View in: PubMed
  89. Senapedis WT, Kennedy CJ, Boyle PM, Silver PA. Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. Mol Biol Cell. 2011 May 15; 22(10):1791-805. PMID: 21460183; PMCID: PMC3093329.
    View in: PubMed, PubMed Central
  90. Waks Z, Silver PA. Nuclear origins of cell-to-cell variability. Cold Spring Harb Symp Quant Biol. 2010; 75:87-94. PMID: 21447820.
    View in: PubMed
  91. Smolke CD, Silver PA. Informing biological design by integration of systems and synthetic biology. Cell. 2011 Mar 18; 144(6):855-9. PMID: 21414477; PMCID: PMC3173940.
    View in: PubMed, PubMed Central
  92. Burrill DR, Silver PA. Synthetic circuit identifies subpopulations with sustained memory of DNA damage. Genes Dev. 2011 Mar 01; 25(5):434-9. PMID: 21363961; PMCID: PMC3049284.
    View in: PubMed, PubMed Central
  93. Ducat DC, Sachdeva G, Silver PA. Rewiring hydrogenase-dependent redox circuits in cyanobacteria. Proc Natl Acad Sci U S A. 2011 Mar 08; 108(10):3941-6. PMID: 21368150; PMCID: PMC3053959.
    View in: PubMed, PubMed Central
  94. Ducat DC, Way JC, Silver PA. Engineering cyanobacteria to generate high-value products. Trends Biotechnol. 2011 Feb; 29(2):95-103. PMID: 21211860.
    View in: PubMed
  95. Wintermute EH, Silver PA. Dynamics in the mixed microbial concourse. Genes Dev. 2010 Dec 01; 24(23):2603-14. PMID: 21123647; PMCID: PMC2994034.
    View in: PubMed, PubMed Central
  96. Wang Q, Silver PA. Genome-wide RNAi screen discovers functional coupling of alternative splicing and cell cycle control to apoptosis regulation. Cell Cycle. 2010 Nov 15; 9(22):4419-21. PMID: 21088477; PMCID: PMC3142361.
    View in: PubMed, PubMed Central
  97. Agapakis CM, Silver PA. Modular electron transfer circuits for synthetic biology: insulation of an engineered biohydrogen pathway. Bioeng Bugs. 2010 Nov-Dec; 1(6):413-8. PMID: 21468209; PMCID: PMC3056092.
    View in: PubMed, PubMed Central
  98. Aldaye FA, Senapedis WT, Silver PA, Way JC. A structurally tunable DNA-based extracellular matrix. J Am Chem Soc. 2010 Oct 27; 132(42):14727-9. PMID: 20925350; PMCID: PMC3092387.
    View in: PubMed, PubMed Central
  99. Wintermute EH, Silver PA. Emergent cooperation in microbial metabolism. Mol Syst Biol. 2010 Sep 07; 6:407. PMID: 20823845; PMCID: PMC2964121.
    View in: PubMed, PubMed Central
  100. Moore MJ, Wang Q, Kennedy CJ, Silver PA. An alternative splicing network links cell-cycle control to apoptosis. Cell. 2010 Aug 20; 142(4):625-36. PMID: 20705336; PMCID: PMC2924962.
    View in: PubMed, PubMed Central
  101. Silver PA. Knowing when to change: reprogramming (my) life. Nat Cell Biol. 2010 Aug; 12(8):730. PMID: 20680000.
    View in: PubMed
  102. Niederholtmeyer H, Wolfstädter BT, Savage DF, Silver PA, Way JC. Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol. 2010 Jun; 76(11):3462-6. PMID: 20363793; PMCID: PMC2876443.
    View in: PubMed, PubMed Central
  103. Savage DF, Afonso B, Chen AH, Silver PA. Spatially ordered dynamics of the bacterial carbon fixation machinery. Science. 2010 Mar 05; 327(5970):1258-61. PMID: 20203050.
    View in: PubMed
  104. Agapakis CM, Ducat DC, Boyle PM, Wintermute EH, Way JC, Silver PA. Insulation of a synthetic hydrogen metabolism circuit in bacteria. J Biol Eng. 2010 Feb 25; 4:3. PMID: 20184755; PMCID: PMC2847965.
    View in: PubMed, PubMed Central
  105. Afonso B, Silver PA, Ajo-Franklin CM. A synthetic circuit for selectively arresting daughter cells to create aging populations. Nucleic Acids Res. 2010 May; 38(8):2727-35. PMID: 20150416; PMCID: PMC2860115.
    View in: PubMed, PubMed Central
  106. Taylor ND, Way JC, Silver PA, Cironi P. Anti-glycophorin single-chain Fv fusion to low-affinity mutant erythropoietin improves red blood cell-lineage specificity. Protein Eng Des Sel. 2010 Apr; 23(4):251-60. PMID: 20083493.
    View in: PubMed
  107. Burrill DR, Silver PA. Making cellular memories. Cell. 2010 Jan 08; 140(1):13-8. PMID: 20085698; PMCID: PMC2882105.
    View in: PubMed, PubMed Central
  108. Haynes KA, Silver PA. Eukaryotic systems broaden the scope of synthetic biology. J Cell Biol. 2009 Nov 30; 187(5):589-96. PMID: 19948487; PMCID: PMC2806586.
    View in: PubMed, PubMed Central
  109. Farny NG, Kedersha NL, Silver PA. Metazoan stress granule assembly is mediated by P-eIF2alpha-dependent and -independent mechanisms. RNA. 2009 Oct; 15(10):1814-21. PMID: 19661161; PMCID: PMC2743051.
    View in: PubMed, PubMed Central
  110. Kennedy CJ, Boyle PM, Waks Z, Silver PA. Systems-level engineering of nonfermentative metabolism in yeast. Genetics. 2009 Sep; 183(1):385-97. PMID: 19564482; PMCID: PMC2746161.
    View in: PubMed, PubMed Central
  111. Agapakis CM, Silver PA. Synthetic biology: exploring and exploiting genetic modularity through the design of novel biological networks. Mol Biosyst. 2009 Jul; 5(7):704-13. PMID: 19562109.
    View in: PubMed
  112. Hurt JA, Obar RA, Zhai B, Farny NG, Gygi SP, Silver PA. A conserved CCCH-type zinc finger protein regulates mRNA nuclear adenylation and export. J Cell Biol. 2009 Apr 20; 185(2):265-77. PMID: 19364924; PMCID: PMC2700372.
    View in: PubMed, PubMed Central
  113. Boyle PM, Silver PA. Harnessing nature's toolbox: regulatory elements for synthetic biology. J R Soc Interface. 2009 Aug 06; 6 Suppl 4:S535-46. PMID: 19324675; PMCID: PMC2843961.
    View in: PubMed, PubMed Central
  114. Waks Z, Silver PA. Engineering a synthetic dual-organism system for hydrogen production. Appl Environ Microbiol. 2009 Apr; 75(7):1867-75. PMID: 19201964; PMCID: PMC2663214.
    View in: PubMed, PubMed Central
  115. Lee SI, Dudley AM, Drubin D, Silver PA, Krogan NJ, Pe'er D, Koller D. Learning a prior on regulatory potential from eQTL data. PLoS Genet. 2009 Jan; 5(1):e1000358. PMID: 19180192; PMCID: PMC2627940.
    View in: PubMed, PubMed Central
  116. Hurt JA, Silver PA. mRNA nuclear export and human disease. Dis Model Mech. 2008 Sep-Oct; 1(2-3):103-8. PMID: 19048072; PMCID: PMC2562179.
    View in: PubMed, PubMed Central
  117. Swinburne IA, Miguez DG, Landgraf D, Silver PA. Intron length increases oscillatory periods of gene expression in animal cells. Genes Dev. 2008 Sep 01; 22(17):2342-6. PMID: 18703678; PMCID: PMC2532923.
    View in: PubMed, PubMed Central
  118. Swinburne IA, Silver PA. Intron delays and transcriptional timing during development. Dev Cell. 2008 Mar; 14(3):324-30. PMID: 18331713; PMCID: PMC2825037.
    View in: PubMed, PubMed Central
  119. Brown CR, Kennedy CJ, Delmar VA, Forbes DJ, Silver PA. Global histone acetylation induces functional genomic reorganization at mammalian nuclear pore complexes. Genes Dev. 2008 Mar 01; 22(5):627-39. PMID: 18316479; PMCID: PMC2259032.
    View in: PubMed, PubMed Central
  120. Cironi P, Swinburne IA, Silver PA. Enhancement of cell type specificity by quantitative modulation of a chimeric ligand. J Biol Chem. 2008 Mar 28; 283(13):8469-76. PMID: 18230610; PMCID: PMC2417160.
    View in: PubMed, PubMed Central
  121. Savage DF, Way J, Silver PA. Defossiling fuel: how synthetic biology can transform biofuel production. ACS Chem Biol. 2008 Jan 18; 3(1):13-6. PMID: 18205286.
    View in: PubMed
  122. Komili S, Silver PA. Coupling and coordination in gene expression processes: a systems biology view. Nat Rev Genet. 2008 Jan; 9(1):38-48. PMID: 18071322.
    View in: PubMed
  123. Frietze S, Lupien M, Silver PA, Brown M. CARM1 regulates estrogen-stimulated breast cancer growth through up-regulation of E2F1. Cancer Res. 2008 Jan 01; 68(1):301-6. PMID: 18172323.
    View in: PubMed
  124. Farny NG, Hurt JA, Silver PA. Definition of global and transcript-specific mRNA export pathways in metazoans. Genes Dev. 2008 Jan 01; 22(1):66-78. PMID: 18086857; PMCID: PMC2151015.
    View in: PubMed, PubMed Central
  125. Moore MJ, Silver PA. Global analysis of mRNA splicing. RNA. 2008 Feb; 14(2):197-203. PMID: 18083834; PMCID: PMC2212243.
    View in: PubMed, PubMed Central
  126. Komili S, Farny NG, Roth FP, Silver PA. Functional specificity among ribosomal proteins regulates gene expression. Cell. 2007 Nov 02; 131(3):557-71. PMID: 17981122; PMCID: PMC2443060.
    View in: PubMed, PubMed Central
  127. Ajo-Franklin CM, Drubin DA, Eskin JA, Gee EP, Landgraf D, Phillips I, Silver PA. Rational design of memory in eukaryotic cells. Genes Dev. 2007 Sep 15; 21(18):2271-6. PMID: 17875664; PMCID: PMC1973140.
    View in: PubMed, PubMed Central
  128. Silver PA, Way JC. Molecular systems biology in drug development. Clin Pharmacol Ther. 2007 Nov; 82(5):586-90. PMID: 17637784.
    View in: PubMed
  129. McKee AE, Silver PA. Systems perspectives on mRNA processing. Cell Res. 2007 Jul; 17(7):581-90. PMID: 17621309.
    View in: PubMed
  130. Zhou Z, Cironi P, Lin AJ, Xu Y, Hrvatin S, Golan DE, Silver PA, Walsh CT, Yin J. Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases. ACS Chem Biol. 2007 May 22; 2(5):337-46. PMID: 17465518.
    View in: PubMed
  131. Silver PA. Why do so few women speak at science meetings? Nature. 2007 Apr 19; 446(7138):856. PMID: 17443161.
    View in: PubMed
  132. Brown CR, Silver PA. Transcriptional regulation at the nuclear pore complex. Curr Opin Genet Dev. 2007 Apr; 17(2):100-6. PMID: 17317147.
    View in: PubMed
  133. Drubin DA, Way JC, Silver PA. Designing biological systems. Genes Dev. 2007 Feb 01; 21(3):242-54. PMID: 17289915.
    View in: PubMed
  134. McKee AE, Neretti N, Carvalho LE, Meyer CA, Fox EA, Brodsky AS, Silver PA. Exon expression profiling reveals stimulus-mediated exon use in neural cells. Genome Biol. 2007; 8(8):R159. PMID: 17683528; PMCID: PMC2374990.
    View in: PubMed, PubMed Central
  135. Moore MJ, Schwartzfarb EM, Silver PA, Yu MC. Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing. Mol Cell. 2006 Dec 28; 24(6):903-15. PMID: 17189192.
    View in: PubMed
  136. Yu MC, Lamming DW, Eskin JA, Sinclair DA, Silver PA. The role of protein arginine methylation in the formation of silent chromatin. Genes Dev. 2006 Dec 01; 20(23):3249-54. PMID: 17158743; PMCID: PMC1686602.
    View in: PubMed, PubMed Central
  137. Tsankov AM, Brown CR, Yu MC, Win MZ, Silver PA, Casolari JM. Communication between levels of transcriptional control improves robustness and adaptivity. Mol Syst Biol. 2006; 2:65. PMID: 17130867; PMCID: PMC1682026.
    View in: PubMed, PubMed Central
  138. Goffin L, Vodala S, Fraser C, Ryan J, Timms M, Meusburger S, Catimel B, Nice EC, Silver PA, Xiao CY, Jans DA, Gething MJ. The unfolded protein response transducer Ire1p contains a nuclear localization sequence recognized by multiple beta importins. Mol Biol Cell. 2006 Dec; 17(12):5309-23. PMID: 17035634.
    View in: PubMed
  139. Damelin M, Silver P. Experimental Design for In Vivo FRET Analysis. CSH Protoc. 2006 Oct 01; 2006(5). PMID: 22485930.
    View in: PubMed
  140. Damelin M, Silver P. Equipment for In Vivo FRET Analysis. CSH Protoc. 2006 Oct 01; 2006(5). PMID: 22485936.
    View in: PubMed
  141. Damelin M, Silver P. An Explanation of Symbols Used for Analysis of FRET Data. CSH Protoc. 2006 Oct 01; 2006(5). PMID: 22485937.
    View in: PubMed
  142. Damelin M, Silver P. Analysis of Protein Interactions In Vivo with Fluorescence Resonance Energy Transfer (FRET). CSH Protoc. 2006 Oct 01; 2006(5). PMID: 22485962.
    View in: PubMed
  143. Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF, Wang Q, Bekiranov S, Sementchenko V, Fox EA, Silver PA, Gingeras TR, Liu XS, Brown M. Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006 Nov; 38(11):1289-97. PMID: 17013392.
    View in: PubMed
  144. Arbiser JL, Kau T, Konar M, Narra K, Ramchandran R, Summers SA, Vlahos CJ, Ye K, Perry BN, Matter W, Fischl A, Cook J, Silver PA, Bain J, Cohen P, Whitmire D, Furness S, Govindarajan B, Bowen JP. Solenopsin, the alkaloidal component of the fire ant (Solenopsis invicta), is a naturally occurring inhibitor of phosphatidylinositol-3-kinase signaling and angiogenesis. Blood. 2007 Jan 15; 109(2):560-5. PMID: 16990598; PMCID: PMC1785094.
    View in: PubMed, PubMed Central
  145. Auld KL, Silver PA. Transcriptional regulation by the proteasome as a mechanism for cellular protein homeostasis. Cell Cycle. 2006 Jul; 5(14):1503-5. PMID: 16861887.
    View in: PubMed
  146. Auld KL, Hitchcock AL, Doherty HK, Frietze S, Huang LS, Silver PA. The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae. Genetics. 2006 Sep; 174(1):215-27. PMID: 16816426; PMCID: PMC1569774.
    View in: PubMed, PubMed Central
  147. Swinburne IA, Meyer CA, Liu XS, Silver PA, Brodsky AS. Genomic localization of RNA binding proteins reveals links between pre-mRNA processing and transcription. Genome Res. 2006 Jul; 16(7):912-21. PMID: 16769980; PMCID: PMC1484458.
    View in: PubMed, PubMed Central
  148. McKee AE, Silver PA. Systems biology of gene regulation fulfills its promise. Genome Biol. 2006; 7(5):316. PMID: 16719937; PMCID: PMC1779525.
    View in: PubMed, PubMed Central
  149. Brown CR, Silver PA. Pore-ing the right dose. Nat Cell Biol. 2006 May; 8(5):430-1. PMID: 16691206.
    View in: PubMed
  150. Drubin DA, Garakani AM, Silver PA. Motion as a phenotype: the use of live-cell imaging and machine visual screening to characterize transcription-dependent chromosome dynamics. BMC Cell Biol. 2006 Apr 24; 7:19. PMID: 16635267; PMCID: PMC1488840.
    View in: PubMed, PubMed Central
  151. Auld KL, Brown CR, Casolari JM, Komili S, Silver PA. Genomic association of the proteasome demonstrates overlapping gene regulatory activity with transcription factor substrates. Mol Cell. 2006 Mar 17; 21(6):861-71. PMID: 16543154.
    View in: PubMed
  152. Bachand F, Lackner DH, Bähler J, Silver PA. Autoregulation of ribosome biosynthesis by a translational response in fission yeast. Mol Cell Biol. 2006 Mar; 26(5):1731-42. PMID: 16478994.
    View in: PubMed
  153. Hitchcock AL, Kahana JA, Silver PA. The uses of green fluorescent protein in yeasts. Methods Biochem Anal. 2006; 47:179-201. PMID: 16335714.
    View in: PubMed
  154. Gama-Carvalho M, Barbosa-Morais NL, Brodsky AS, Silver PA, Carmo-Fonseca M. Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors. Genome Biol. 2006; 7(11):R113. PMID: 17137510; PMCID: PMC1794580.
    View in: PubMed, PubMed Central
  155. Vandegraaff N, Devroe E, Turlure F, Silver PA, Engelman A. Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication. Virology. 2006 Mar 15; 346(2):415-26. PMID: 16337983.
    View in: PubMed
  156. Robinson MA, Park S, Sun ZY, Silver PA, Wagner G, Hogle JM. Multiple conformations in the ligand-binding site of the yeast nuclear pore-targeting domain of Nup116p. J Biol Chem. 2005 Oct 21; 280(42):35723-32. PMID: 16105837.
    View in: PubMed
  157. McKee AE, Minet E, Stern C, Riahi S, Stiles CD, Silver PA. A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. BMC Dev Biol. 2005 Jul 20; 5:14. PMID: 16033648; PMCID: PMC1199591.
    View in: PubMed, PubMed Central
  158. Brodsky AS, Meyer CA, Swinburne IA, Hall G, Keenan BJ, Liu XS, Fox EA, Silver PA. Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells. Genome Biol. 2005; 6(8):R64. PMID: 16086846; PMCID: PMC1273631.
    View in: PubMed, PubMed Central
  159. Carroll JS, Liu XS, Brodsky AS, Li W, Meyer CA, Szary AJ, Eeckhoute J, Shao W, Hestermann EV, Geistlinger TR, Fox EA, Silver PA, Brown M. Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell. 2005 Jul 15; 122(1):33-43. PMID: 16009131.
    View in: PubMed
  160. Park S, Isaacson R, Kim HT, Silver PA, Wagner G. Ufd1 exhibits the AAA-ATPase fold with two distinct ubiquitin interaction sites. Structure. 2005 Jul; 13(7):995-1005. PMID: 16004872.
    View in: PubMed
  161. Casolari JM, Brown CR, Drubin DA, Rando OJ, Silver PA. Developmentally induced changes in transcriptional program alter spatial organization across chromosomes. Genes Dev. 2005 May 15; 19(10):1188-98. PMID: 15905407; PMCID: PMC1132005.
    View in: PubMed, PubMed Central
  162. Schroeder FC, Kau TR, Silver PA, Clardy J. The psammaplysenes, specific inhibitors of FOXO1a nuclear export. J Nat Prod. 2005 Apr; 68(4):574-6. PMID: 15844952.
    View in: PubMed
  163. Devroe E, Silver PA, Engelman A. HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases. Virology. 2005 Jan 05; 331(1):181-9. PMID: 15582665.
    View in: PubMed
  164. Lu R, Limón A, Devroe E, Silver PA, Cherepanov P, Engelman A. Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. J Virol. 2004 Dec; 78(23):12735-46. PMID: 15542626; PMCID: PMC525011.
    View in: PubMed, PubMed Central
  165. Hieronymus H, Silver PA. A systems view of mRNP biology. Genes Dev. 2004 Dec 01; 18(23):2845-60. PMID: 15574591.
    View in: PubMed
  166. Hieronymus H, Yu MC, Silver PA. Genome-wide mRNA surveillance is coupled to mRNA export. Genes Dev. 2004 Nov 01; 18(21):2652-62. PMID: 15489286; PMCID: PMC525545.
    View in: PubMed, PubMed Central
  167. Cherepanov P, Devroe E, Silver PA, Engelman A. Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. J Biol Chem. 2004 Nov 19; 279(47):48883-92. PMID: 15371438.
    View in: PubMed
  168. Turlure F, Devroe E, Silver PA, Engelman A. Human cell proteins and human immunodeficiency virus DNA integration. Front Biosci. 2004 Sep 01; 9:3187-208. PMID: 15353349.
    View in: PubMed
  169. Yu MC, Bachand F, McBride AE, Komili S, Casolari JM, Silver PA. Arginine methyltransferase affects interactions and recruitment of mRNA processing and export factors. Genes Dev. 2004 Aug 15; 18(16):2024-35. PMID: 15314027; PMCID: PMC514182.
    View in: PubMed, PubMed Central
  170. Bachand F, Silver PA. PRMT3 is a ribosomal protein methyltransferase that affects the cellular levels of ribosomal subunits. EMBO J. 2004 Jul 07; 23(13):2641-50. PMID: 15175657; PMCID: PMC449775.
    View in: PubMed, PubMed Central
  171. McKee AE, Silver PA. REF-ereeing the cytoplasmic fate of mRNA via nuclear export. Dev Cell. 2004 Jun; 6(6):740-2. PMID: 15177019.
    View in: PubMed
  172. Casolari JM, Brown CR, Komili S, West J, Hieronymus H, Silver PA. Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization. Cell. 2004 May 14; 117(4):427-39. PMID: 15137937.
    View in: PubMed
  173. Casolari JM, Silver PA. Guardian at the gate: preventing unspliced pre-mRNA export. Trends Cell Biol. 2004 May; 14(5):222-5. PMID: 15130577.
    View in: PubMed
  174. Devroe E, Erdjument-Bromage H, Tempst P, Silver PA. Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. J Biol Chem. 2004 Jun 04; 279(23):24444-51. PMID: 15067004.
    View in: PubMed
  175. Devroe E, Silver PA. Therapeutic potential of retroviral RNAi vectors. Expert Opin Biol Ther. 2004 Mar; 4(3):319-27. PMID: 15006726.
    View in: PubMed
  176. Kau TR, Way JC, Silver PA. Nuclear transport and cancer: from mechanism to intervention. Nat Rev Cancer. 2004 Feb; 4(2):106-17. PMID: 14732865.
    View in: PubMed
  177. Jiang Y, Minet E, Zhang Z, Silver PA, Bai M. Modulation of interprotomer relationships is important for activation of dimeric calcium-sensing receptor. J Biol Chem. 2004 Apr 02; 279(14):14147-56. PMID: 14729680.
    View in: PubMed
  178. Kau TR, Schroeder F, Ramaswamy S, Wojciechowski CL, Zhao JJ, Roberts TM, Clardy J, Sellers WR, Silver PA. A chemical genetic screen identifies inhibitors of regulated nuclear export of a Forkhead transcription factor in PTEN-deficient tumor cells. Cancer Cell. 2003 Dec; 4(6):463-76. PMID: 14706338.
    View in: PubMed
  179. Haggarty SJ, Koeller KM, Kau TR, Silver PA, Roberge M, Schreiber SL. Small molecule modulation of the human chromatid decatenation checkpoint. Chem Biol. 2003 Dec; 10(12):1267-79. PMID: 14700634.
    View in: PubMed
  180. Hitchcock AL, Auld K, Gygi SP, Silver PA. A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. Proc Natl Acad Sci U S A. 2003 Oct 28; 100(22):12735-40. PMID: 14557538; PMCID: PMC240687.
    View in: PubMed, PubMed Central
  181. Devroe E, Engelman A, Silver PA. Intracellular transport of human immunodeficiency virus type 1 integrase. J Cell Sci. 2003 Nov 01; 116(Pt 21):4401-8. PMID: 13130095.
    View in: PubMed
  182. Brodsky AS, Johnston AP, Trau M, Silver PA. Analysis of RNA-protein interactions by flow cytometry. Curr Opin Mol Ther. 2003 Jun; 5(3):235-40. PMID: 12870432.
    View in: PubMed
  183. Lei EP, Stern CA, Fahrenkrog B, Krebber H, Moy TI, Aebi U, Silver PA. Sac3 is an mRNA export factor that localizes to cytoplasmic fibrils of nuclear pore complex. Mol Biol Cell. 2003 Mar; 14(3):836-47. PMID: 12631707.
    View in: PubMed
  184. Kau TR, Silver PA. Nuclear transport as a target for cell growth. Drug Discov Today. 2003 Jan 15; 8(2):78-85. PMID: 12565010.
    View in: PubMed
  185. Hieronymus H, Silver PA. Genome-wide analysis of RNA-protein interactions illustrates specificity of the mRNA export machinery. Nat Genet. 2003 Feb; 33(2):155-61. PMID: 12524544.
    View in: PubMed
  186. Beilharz T, Egan B, Silver PA, Hofmann K, Lithgow T. Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae. J Biol Chem. 2003 Mar 07; 278(10):8219-23. PMID: 12514182.
    View in: PubMed
  187. Damelin M, Silver PA. In situ analysis of spatial relationships between proteins of the nuclear pore complex. Biophys J. 2002 Dec; 83(6):3626-36. PMID: 12496130; PMCID: PMC1302438.
    View in: PubMed, PubMed Central
  188. Brodsky AS, Silver PA. A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry. Mol Cell Proteomics. 2002 Dec; 1(12):922-9. PMID: 12543929.
    View in: PubMed
  189. Lei EP, Silver PA. Intron status and 3'-end formation control cotranscriptional export of mRNA. Genes Dev. 2002 Nov 01; 16(21):2761-6. PMID: 12414728; PMCID: PMC187473.
    View in: PubMed, PubMed Central
  190. Limón A, Devroe E, Lu R, Ghory HZ, Silver PA, Engelman A. Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import. J Virol. 2002 Nov; 76(21):10598-607. PMID: 12368302; PMCID: PMC136649.
    View in: PubMed, PubMed Central
  191. Devroe E, Silver PA. Retrovirus-delivered siRNA. BMC Biotechnol. 2002 Aug 28; 2:15. PMID: 12199908; PMCID: PMC126223.
    View in: PubMed, PubMed Central
  192. Moy TI, Silver PA. Requirements for the nuclear export of the small ribosomal subunit. J Cell Sci. 2002 Jul 15; 115(Pt 14):2985-95. PMID: 12082158.
    View in: PubMed
  193. Moy TI, Boettner D, Rhodes JC, Silver PA, Askew DS. Identification of a role for Saccharomyces cerevisiae Cgr1p in pre-rRNA processing and 60S ribosome subunit synthesis. Microbiology. 2002 Apr; 148(Pt 4):1081-90. PMID: 11932453.
    View in: PubMed
  194. Damelin M, Simon I, Moy TI, Wilson B, Komili S, Tempst P, Roth FP, Young RA, Cairns BR, Silver PA. The genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress. Mol Cell. 2002 Mar; 9(3):563-73. PMID: 11931764.
    View in: PubMed
  195. Lei EP, Silver PA. Protein and RNA export from the nucleus. Dev Cell. 2002 Mar; 2(3):261-72. PMID: 11879632.
    View in: PubMed
  196. Valentini SR, Casolari JM, Oliveira CC, Silver PA, McBride AE. Genetic interactions of yeast eukaryotic translation initiation factor 5A (eIF5A) reveal connections to poly(A)-binding protein and protein kinase C signaling. Genetics. 2002 Feb; 160(2):393-405. PMID: 11861547; PMCID: PMC1461981.
    View in: PubMed, PubMed Central
  197. Brodsky AS, Silver PA. Identifying proteins that affect mRNA localization in living cells. Methods. 2002 Feb; 26(2):151-5. PMID: 12054891.
    View in: PubMed
  198. Damelin M, Silver PA, Corbett AH. Nuclear protein transport. Methods Enzymol. 2002; 351:587-607. PMID: 12073370.
    View in: PubMed
  199. Hitchcock AL, Krebber H, Frietze S, Lin A, Latterich M, Silver PA. The conserved npl4 protein complex mediates proteasome-dependent membrane-bound transcription factor activation. Mol Biol Cell. 2001 Oct; 12(10):3226-41. PMID: 11598205.
    View in: PubMed
  200. Lei EP, Krebber H, Silver PA. Messenger RNAs are recruited for nuclear export during transcription. Genes Dev. 2001 Jul 15; 15(14):1771-82. PMID: 11459827; PMCID: PMC312744.
    View in: PubMed, PubMed Central
  201. McBride AE, Silver PA. State of the arg: protein methylation at arginine comes of age. Cell. 2001 Jul 13; 106(1):5-8. PMID: 11461695.
    View in: PubMed
  202. Tham WH, Wyithe JS, Ko Ferrigno P, Silver PA, Zakian VA. Localization of yeast telomeres to the nuclear periphery is separable from transcriptional repression and telomere stability functions. Mol Cell. 2001 Jul; 8(1):189-99. PMID: 11511372.
    View in: PubMed
  203. Kahana JA, Silver PA. Use of the A. victoria green fluorescent protein to study protein dynamics in vivo. Curr Protoc Mol Biol. 2001 May; Chapter 9:Unit9.7C. PMID: 18265287.
    View in: PubMed
  204. Kahana JA, Silver PA. Use of the A. victoria green fluorescent protein to study protein dynamics in vivo. Curr Protoc Neurosci. 2001 May; Chapter 5:Unit 5.15. PMID: 18428498.
    View in: PubMed
  205. Kahana JA, Silver PA. Use of the A. victoria green fluorescent protein to study protein dynamics in vivo. Curr Protoc Pharmacol. 2001 May; Chapter 6:Unit 6.6. PMID: 21965077.
    View in: PubMed
  206. Hood JK, Hwang WW, Silver PA. The Saccharomyces cerevisiae cyclin Clb2p is targeted to multiple subcellular locations by cis- and trans-acting determinants. J Cell Sci. 2001 Feb; 114(Pt 3):589-97. PMID: 11171327.
    View in: PubMed
  207. Colas P, Cohen B, Ko Ferrigno P, Silver PA, Brent R. Targeted modification and transportation of cellular proteins. Proc Natl Acad Sci U S A. 2000 Dec 05; 97(25):13720-5. PMID: 11106396; PMCID: PMC17642.
    View in: PubMed, PubMed Central
  208. Weiss VH, McBride AE, Soriano MA, Filman DJ, Silver PA, Hogle JM. The structure and oligomerization of the yeast arginine methyltransferase, Hmt1. Nat Struct Biol. 2000 Dec; 7(12):1165-71. PMID: 11101900.
    View in: PubMed
  209. Brodsky AS, Silver PA. Pre-mRNA processing factors are required for nuclear export. RNA. 2000 Dec; 6(12):1737-49. PMID: 11142374; PMCID: PMC1370044.
    View in: PubMed, PubMed Central
  210. Stage-Zimmermann T, Schmidt U, Silver PA. Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell. 2000 Nov; 11(11):3777-89. PMID: 11071906.
    View in: PubMed
  211. Prinz WA, Grzyb L, Veenhuis M, Kahana JA, Silver PA, Rapoport TA. Mutants affecting the structure of the cortical endoplasmic reticulum in Saccharomyces cerevisiae. J Cell Biol. 2000 Aug 07; 150(3):461-74. PMID: 10931860; PMCID: PMC2175198.
    View in: PubMed, PubMed Central
  212. Shen EC, Stage-Zimmermann T, Chui P, Silver PA. 7The yeast mRNA-binding protein Npl3p interacts with the cap-binding complex. J Biol Chem. 2000 Aug 04; 275(31):23718-24. PMID: 10823828.
    View in: PubMed
  213. Hood JK, Silver PA. Diverse nuclear transport pathways regulate cell proliferation and oncogenesis. Biochim Biophys Acta. 2000 Jul 31; 1471(1):M31-41. PMID: 10967423.
    View in: PubMed
  214. Ferrigno P, Silver PA. Polyglutamine expansions: proteolysis, chaperones, and the dangers of promiscuity. Neuron. 2000 Apr; 26(1):9-12. PMID: 10798387.
    View in: PubMed
  215. Hood JK, Casolari JM, Silver PA. Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-alpha export. J Cell Sci. 2000 Apr; 113 ( Pt 8):1471-80. PMID: 10725229.
    View in: PubMed
  216. Jones AL, Quimby BB, Hood JK, Ferrigno P, Keshava PH, Silver PA, Corbett AH. SAC3 may link nuclear protein export to cell cycle progression. Proc Natl Acad Sci U S A. 2000 Mar 28; 97(7):3224-9. PMID: 10716708; PMCID: PMC16220.
    View in: PubMed, PubMed Central
  217. McBride AE, Weiss VH, Kim HK, Hogle JM, Silver PA. Analysis of the yeast arginine methyltransferase Hmt1p/Rmt1p and its in vivo function. Cofactor binding and substrate interactions. J Biol Chem. 2000 Feb 04; 275(5):3128-36. PMID: 10652296.
    View in: PubMed
  218. Damelin M, Silver PA. Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex. Mol Cell. 2000 Jan; 5(1):133-40. PMID: 10678175.
    View in: PubMed
  219. Krebber H, Silver PA. Directing proteins to nucleus by fusion to nuclear localization signal tags. Methods Enzymol. 2000; 327:283-96. PMID: 11044991.
    View in: PubMed
  220. Ferrigno P, Silver PA. Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival. Oncogene. 1999 Nov 01; 18(45):6129-34. PMID: 10557104.
    View in: PubMed
  221. Morehouse H, Buratowski RM, Silver PA, Buratowski S. The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc Natl Acad Sci U S A. 1999 Oct 26; 96(22):12542-7. PMID: 10535958; PMCID: PMC22979.
    View in: PubMed, PubMed Central
  222. Moy TI, Silver PA. Nuclear export of the small ribosomal subunit requires the ran-GTPase cycle and certain nucleoporins. Genes Dev. 1999 Aug 15; 13(16):2118-33. PMID: 10465789; PMCID: PMC316945.
    View in: PubMed, PubMed Central
  223. Krebber H, Taura T, Lee MS, Silver PA. Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export. Genes Dev. 1999 Aug 01; 13(15):1994-2004. PMID: 10444597; PMCID: PMC316916.
    View in: PubMed, PubMed Central
  224. Zeng X, Kahana JA, Silver PA, Morphew MK, McIntosh JR, Fitch IT, Carbon J, Saunders WS. Slk19p is a centromere protein that functions to stabilize mitotic spindles. J Cell Biol. 1999 Jul 26; 146(2):415-25. PMID: 10427094; PMCID: PMC3206577.
    View in: PubMed, PubMed Central
  225. Brodsky AS, Silver PA. Nuclear transport HEATs up. Nat Cell Biol. 1999 Jul; 1(3):E66-7. PMID: 10559913.
    View in: PubMed
  226. Hood JK, Silver PA. In or out? Regulating nuclear transport. Curr Opin Cell Biol. 1999 Apr; 11(2):241-7. PMID: 10209150.
    View in: PubMed
  227. Defossez PA, Prusty R, Kaeberlein M, Lin SJ, Ferrigno P, Silver PA, Keil RL, Guarente L. Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol Cell. 1999 Apr; 3(4):447-55. PMID: 10230397.
    View in: PubMed
  228. Valentini SR, Weiss VH, Silver PA. Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation. RNA. 1999 Feb; 5(2):272-80. PMID: 10024178; PMCID: PMC1369758.
    View in: PubMed, PubMed Central
  229. Seedorf M, Damelin M, Kahana J, Taura T, Silver PA. Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase. Mol Cell Biol. 1999 Feb; 19(2):1547-57. PMID: 9891088; PMCID: PMC116083.
    View in: PubMed, PubMed Central
  230. Ferrigno P, Silver PA. Analysis of nuclear transport in vivo. Methods Cell Biol. 1999; 58:107-22. PMID: 9891377.
    View in: PubMed
  231. Hood JK, Silver PA. Cse1p is required for export of Srp1p/importin-alpha from the nucleus in Saccharomyces cerevisiae. J Biol Chem. 1998 Dec 25; 273(52):35142-6. PMID: 9857050.
    View in: PubMed
  232. Silberstein S, Schlenstedt G, Silver PA, Gilmore R. A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum. J Cell Biol. 1998 Nov 16; 143(4):921-33. PMID: 9817751; PMCID: PMC2132949.
    View in: PubMed, PubMed Central
  233. Ferrigno P, Posas F, Koepp D, Saito H, Silver PA. Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin beta homologs NMD5 and XPO1. EMBO J. 1998 Oct 01; 17(19):5606-14. PMID: 9755161; PMCID: PMC1170889.
    View in: PubMed, PubMed Central
  234. Kahana JA, Schlenstedt G, Evanchuk DM, Geiser JR, Hoyt MA, Silver PA. The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Mol Biol Cell. 1998 Jul; 9(7):1741-56. PMID: 9658168; PMCID: PMC25412.
    View in: PubMed, PubMed Central
  235. Finley D, Tanaka K, Mann C, Feldmann H, Hochstrasser M, Vierstra R, Johnston S, Hampton R, Haber J, Mccusker J, Silver P, Frontali L, Thorsness P, Varshavsky A, Byers B, Madura K, Reed SI, Wolf D, Jentsch S, Sommer T, Baumeister W, Goldberg A, Fried V, Rubin DM, Toh-e A, et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci. 1998 Jul; 23(7):244-5. PMID: 9697412.
    View in: PubMed
  236. Taura T, Krebber H, Silver PA. A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export. Proc Natl Acad Sci U S A. 1998 Jun 23; 95(13):7427-32. PMID: 9636166; PMCID: PMC22639.
    View in: PubMed, PubMed Central
  237. Webb CD, Graumann PL, Kahana JA, Teleman AA, Silver PA, Losick R. Use of time-lapse microscopy to visualize rapid movement of the replication origin region of the chromosome during the cell cycle in Bacillus subtilis. Mol Microbiol. 1998 Jun; 28(5):883-92. PMID: 9663676.
    View in: PubMed
  238. Koepp DM, Silver PA. Nucleocytoplasmic transport and cell proliferation. Biochim Biophys Acta. 1998 Apr 17; 1377(2):M39-47. PMID: 9606975.
    View in: PubMed
  239. Scott HS, Antonarakis SE, Lalioti MD, Rossier C, Silver PA, Henry MF. Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2). Genomics. 1998 Mar 15; 48(3):330-40. PMID: 9545638.
    View in: PubMed
  240. Shen EC, Henry MF, Weiss VH, Valentini SR, Silver PA, Lee MS. Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev. 1998 Mar 01; 12(5):679-91. PMID: 9499403; PMCID: PMC316575.
    View in: PubMed, PubMed Central
  241. Huyett A, Kahana J, Silver P, Zeng X, Saunders WS. The Kar3p and Kip2p motors function antagonistically at the spindle poles to influence cytoplasmic microtubule numbers. J Cell Sci. 1998 Feb; 111 ( Pt 3):295-301. PMID: 9427678.
    View in: PubMed
  242. Vodicka MA, Koepp DM, Silver PA, Emerman M. HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection. Genes Dev. 1998 Jan 15; 12(2):175-85. PMID: 9436978; PMCID: PMC316441.
    View in: PubMed, PubMed Central
  243. Taura T, Schlenstedt G, Silver PA. Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue. J Biol Chem. 1997 Dec 12; 272(50):31877-84. PMID: 9395535.
    View in: PubMed
  244. Clarkson WD, Corbett AH, Paschal BM, Kent HM, McCoy AJ, Gerace L, Silver PA, Stewart M. Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran. J Mol Biol. 1997 Oct 10; 272(5):716-30. PMID: 9368653.
    View in: PubMed
  245. Kessler MM, Henry MF, Shen E, Zhao J, Gross S, Silver PA, Moore CL. Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast. Genes Dev. 1997 Oct 01; 11(19):2545-56. PMID: 9334319; PMCID: PMC316558.
    View in: PubMed, PubMed Central
  246. Seedorf M, Silver PA. Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci U S A. 1997 Aug 05; 94(16):8590-5. PMID: 9238021; PMCID: PMC23031.
    View in: PubMed, PubMed Central
  247. Wong DH, Corbett AH, Kent HM, Stewart M, Silver PA. Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport. Mol Cell Biol. 1997 Jul; 17(7):3755-67. PMID: 9199309; PMCID: PMC232227.
    View in: PubMed, PubMed Central
  248. Corbett AH, Silver PA. Nucleocytoplasmic transport of macromolecules. Microbiol Mol Biol Rev. 1997 Jun; 61(2):193-211. PMID: 9184010; PMCID: PMC232607.
    View in: PubMed, PubMed Central
  249. Campbell KS, Mullane KP, Aksoy IA, Stubdal H, Zalvide J, Pipas JM, Silver PA, Roberts TM, Schaffhausen BS, DeCaprio JA. DnaJ/hsp40 chaperone domain of SV40 large T antigen promotes efficient viral DNA replication. Genes Dev. 1997 May 01; 11(9):1098-110. PMID: 9159391.
    View in: PubMed
  250. Lee MS, Silver PA. RNA movement between the nucleus and the cytoplasm. Curr Opin Genet Dev. 1997 Apr; 7(2):212-9. PMID: 9115427.
    View in: PubMed
  251. Lithgow T, Cuezva JM, Silver PA. Highways for protein delivery to the mitochondria. Trends Biochem Sci. 1997 Apr; 22(4):110-3. PMID: 9149527.
    View in: PubMed
  252. Corbett A, Ferrigno P, Henry M, Kahana J, Koepp D, Lee M, Nguyen L, Schlenstedt G, Seedorf M, Shen E, Taura T, Wong D, Silver P. Genetic analysis of macromolecular transport across the nuclear envelope. Exp Cell Res. 1996 Dec 15; 229(2):212-6. PMID: 8986600.
    View in: PubMed
  253. DeHoratius C, Silver PA. Nuclear transport defects and nuclear envelope alterations are associated with mutation of the Saccharomyces cerevisiae NPL4 gene. Mol Biol Cell. 1996 Nov; 7(11):1835-55. PMID: 8930904; PMCID: PMC276030.
    View in: PubMed, PubMed Central
  254. Koepp DM, Silver PA. A GTPase controlling nuclear trafficking: running the right way or walking RANdomly? Cell. 1996 Oct 04; 87(1):1-4. PMID: 8858141.
    View in: PubMed
  255. Haarer BK, Corbett A, Kweon Y, Petzold AS, Silver P, Brown SS. SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection. Genetics. 1996 Oct; 144(2):495-510. PMID: 8889515; PMCID: PMC1207545.
    View in: PubMed, PubMed Central
  256. Corbett AH, Silver PA. The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo. J Biol Chem. 1996 Aug 02; 271(31):18477-84. PMID: 8702493.
    View in: PubMed
  257. Henry MF, Silver PA. A novel methyltransferase (Hmt1p) modifies poly(A)+-RNA-binding proteins. Mol Cell Biol. 1996 Jul; 16(7):3668-78. PMID: 8668183; PMCID: PMC231362.
    View in: PubMed, PubMed Central
  258. Koepp DM, Wong DH, Corbett AH, Silver PA. Dynamic localization of the nuclear import receptor and its interactions with transport factors. J Cell Biol. 1996 Jun; 133(6):1163-76. PMID: 8682856; PMCID: PMC2120906.
    View in: PubMed, PubMed Central
  259. Lee MS, Henry M, Silver PA. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes Dev. 1996 May 15; 10(10):1233-46. PMID: 8675010.
    View in: PubMed
  260. Henry M, Borland CZ, Bossie M, Silver PA. Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3. Genetics. 1996 Jan; 142(1):103-15. PMID: 8770588; PMCID: PMC1206938.
    View in: PubMed, PubMed Central
  261. Schlenstedt G, Wong DH, Koepp DM, Silver PA. Mutants in a yeast Ran binding protein are defective in nuclear transport. EMBO J. 1995 Nov 01; 14(21):5367-78. PMID: 7489726; PMCID: PMC394646.
    View in: PubMed, PubMed Central
  262. Kahana JA, Schnapp BJ, Silver PA. Kinetics of spindle pole body separation in budding yeast. Proc Natl Acad Sci U S A. 1995 Oct 10; 92(21):9707-11. PMID: 7568202; PMCID: PMC40871.
    View in: PubMed, PubMed Central
  263. Corbett AH, Koepp DM, Schlenstedt G, Lee MS, Hopper AK, Silver PA. Rna1p, a Ran/TC4 GTPase activating protein, is required for nuclear import. J Cell Biol. 1995 Sep; 130(5):1017-26. PMID: 7657689; PMCID: PMC2120547.
    View in: PubMed, PubMed Central
  264. Loeb JD, Schlenstedt G, Pellman D, Kornitzer D, Silver PA, Fink GR. The yeast nuclear import receptor is required for mitosis. Proc Natl Acad Sci U S A. 1995 Aug 15; 92(17):7647-51. PMID: 7644471; PMCID: PMC41202.
    View in: PubMed, PubMed Central
  265. Schlenstedt G, Harris S, Risse B, Lill R, Silver PA. A yeast DnaJ homologue, Scj1p, can function in the endoplasmic reticulum with BiP/Kar2p via a conserved domain that specifies interactions with Hsp70s. J Cell Biol. 1995 May; 129(4):979-88. PMID: 7744969; PMCID: PMC2120480.
    View in: PubMed, PubMed Central
  266. Schlenstedt G, Saavedra C, Loeb JD, Cole CN, Silver PA. The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm. Proc Natl Acad Sci U S A. 1995 Jan 03; 92(1):225-9. PMID: 7816822; PMCID: PMC42850.
    View in: PubMed, PubMed Central
  267. Flach J, Bossie M, Vogel J, Corbett A, Jinks T, Willins DA, Silver PA. A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm. Mol Cell Biol. 1994 Dec; 14(12):8399-407. PMID: 7969175; PMCID: PMC359379.
    View in: PubMed, PubMed Central
  268. Osborne MA, Schlenstedt G, Jinks T, Silver PA. Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol. 1994 May; 125(4):853-66. PMID: 8188751; PMCID: PMC2120077.
    View in: PubMed, PubMed Central
  269. Wiedmann B, Silver P, Schunck WH, Wiedmann M. Overexpression of the ER-membrane protein P-450 CYP52A3 mimics sec mutant characteristics in Saccharomyces cerevisiae. Biochim Biophys Acta. 1993 Dec 12; 1153(2):267-76. PMID: 8274497.
    View in: PubMed
  270. Schlenstedt G, Hurt E, Doye V, Silver PA. Reconstitution of nuclear protein transport with semi-intact yeast cells. J Cell Biol. 1993 Nov; 123(4):785-98. PMID: 8227140; PMCID: PMC2200159.
    View in: PubMed, PubMed Central
  271. Kurihara T, Silver P. Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus. Mol Biol Cell. 1993 Sep; 4(9):919-30. PMID: 8257794; PMCID: PMC275722.
    View in: PubMed, PubMed Central
  272. Silver PA, Way JC. Eukaryotic DnaJ homologs and the specificity of Hsp70 activity. Cell. 1993 Jul 16; 74(1):5-6. PMID: 8334705.
    View in: PubMed
  273. Nelson MK, Kurihara T, Silver PA. Extragenic suppressors of mutations in the cytoplasmic C terminus of SEC63 define five genes in Saccharomyces cerevisiae. Genetics. 1993 May; 134(1):159-73. PMID: 8514125; PMCID: PMC1205419.
    View in: PubMed, PubMed Central
  274. Stochaj U, Bossie MA, van Zee K, Whalen AM, Silver PA. Analysis of conserved binding proteins for nuclear localization sequences. J Cell Sci. 1993 Jan; 104 ( Pt 1):89-95. PMID: 7680661.
    View in: PubMed
  275. Osborne MA, Silver PA. Nucleocytoplasmic transport in the yeast Saccharomyces cerevisiae. Annu Rev Biochem. 1993; 62:219-54. PMID: 8352590.
    View in: PubMed
  276. Stochaj U, Silver P. Nucleocytoplasmic traffic of proteins. Eur J Cell Biol. 1992 Oct; 59(1):1-11. PMID: 1468433.
    View in: PubMed
  277. Bossie MA, Silver PA. Movement of macromolecules between the cytoplasm and the nucleus in yeast. Curr Opin Genet Dev. 1992 Oct; 2(5):768-74. PMID: 1458025.
    View in: PubMed
  278. Bossie MA, DeHoratius C, Barcelo G, Silver P. A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast. Mol Biol Cell. 1992 Aug; 3(8):875-93. PMID: 1392078.
    View in: PubMed
  279. Stochaj U, Silver PA. A conserved phosphoprotein that specifically binds nuclear localization sequences is involved in nuclear import. J Cell Biol. 1992 May; 117(3):473-82. PMID: 1572892; PMCID: PMC2289452.
    View in: PubMed, PubMed Central
  280. Blumberg H, Silver P. A split zinc-finger protein is required for normal yeast growth. Gene. 1991 Oct 30; 107(1):101-10. PMID: 1743508.
    View in: PubMed
  281. Stochaj U, Osborne M, Kurihara T, Silver P. A yeast protein that binds nuclear localization signals: purification localization, and antibody inhibition of binding activity. J Cell Biol. 1991 Jun; 113(6):1243-54. PMID: 2045410; PMCID: PMC2289025.
    View in: PubMed, PubMed Central
  282. Blumberg H, Silver PA. A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast. Nature. 1991 Feb 14; 349(6310):627-30. PMID: 2000136.
    View in: PubMed
  283. Silver PA. How proteins enter the nucleus. Cell. 1991 Feb 08; 64(3):489-97. PMID: 1991319.
    View in: PubMed
  284. Sadler I, Chiang A, Kurihara T, Rothblatt J, Way J, Silver P. A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein. J Cell Biol. 1989 Dec; 109(6 Pt 1):2665-75. PMID: 2556404; PMCID: PMC2115965.
    View in: PubMed, PubMed Central
  285. Silver P, Sadler I, Osborne MA. Yeast proteins that recognize nuclear localization sequences. J Cell Biol. 1989 Sep; 109(3):983-9. PMID: 2670959; PMCID: PMC2115749.
    View in: PubMed, PubMed Central
  286. Nelson M, Silver P. Context affects nuclear protein localization in Saccharomyces cerevisiae. Mol Cell Biol. 1989 Feb; 9(2):384-9. PMID: 2496300; PMCID: PMC362612.
    View in: PubMed, PubMed Central
  287. Silver P, Goodson H. Nuclear protein transport. Crit Rev Biochem Mol Biol. 1989; 24(4):419-35. PMID: 2670452.
    View in: PubMed
  288. Silver PA, Chiang A, Sadler I. Mutations that alter both localization and production of a yeast nuclear protein. Genes Dev. 1988 Jun; 2(6):707-17. PMID: 3138162.
    View in: PubMed
  289. Silver PA, Brent R, Ptashne M. DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae. Mol Cell Biol. 1986 Dec; 6(12):4763-6. PMID: 3099172; PMCID: PMC367265.
    View in: PubMed, PubMed Central
  290. Silver PA, Keegan LP, Ptashne M. Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization. Proc Natl Acad Sci U S A. 1984 Oct; 81(19):5951-5. PMID: 6091123; PMCID: PMC391836.
    View in: PubMed, PubMed Central
  291. Date T, Silver P, Wickner W. Molecular genetics of Escherichia coli leader peptidase. Methods Enzymol. 1983; 97:46-57. PMID: 6318034.
    View in: PubMed
  292. Watts C, Goodman JM, Silver P, Wickner W. Analysis of M13 procoat assembly into membranes in vitro. Methods Enzymol. 1983; 97:130-8. PMID: 6361469.
    View in: PubMed
  293. Watts C, Silver P, Wickner W. Membrane assembly from purified components. II. Assembly of M13 procoat into liposomes reconstituted with purified leader peptidase. Cell. 1981 Aug; 25(2):347-53. PMID: 7026043.
    View in: PubMed
  294. Silver P, Watts C, Wickner W. Membrane assembly from purified components. I. Isolated M13 procoat does not require ribosomes or soluble proteins for processing by membranes. Cell. 1981 Aug; 25(2):341-5. PMID: 7026042.
    View in: PubMed
  295. Silver P, Wickner WT. Role of M13 gene 1 protein in filamentous virus assembly. J Virol. 1980 Jul; 35(1):256-8. PMID: 7411692; PMCID: PMC288803.
    View in: PubMed, PubMed Central
  296. Omar S, Brown MF, Silver P, Schleich T. Histidyl and tyrosyl residue ionization studies of subtilisin Novo. Biochim Biophys Acta. 1979 Jun 19; 578(2):261-8. PMID: 39621.
    View in: PubMed
Local representatives can answer questions about the Profiles website or help with editing a profile or issues with profile data. For assistance with this profile: HMS/HSDM faculty should contact feedbackcatalyst.harvard.edu. For faculty or fellow appointment updates and changes, please ask your appointing department to contact HMS. For fellow personal and demographic information, contact HMS Human Resources at human_resourceshms.harvard.edu. For faculty personal and demographic information, contact HMS Office for Faculty Affairs at facappthms.harvard.edu.
Silver's Networks
Click the
Explore
buttons for more information and interactive visualizations!
Concepts (825)
Explore
_
Co-Authors (56)
Explore
_
Similar People (60)
Explore
_
Same Department 
Explore
_
Physical Neighbors
_