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Last Name

profileEdward Leo Huttlin, PH.D.

TitleInstructor in Cell Biology
InstitutionHarvard Medical School
DepartmentCell Biology
AddressHarvard Medical School
Cell Biology
240 Longwood Ave
Boston MA 02115
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U41HG006673     (GYGI, STEVEN P)Aug 21, 2012 - Jun 30, 2018
Systematic Exploration of the Human Interactome
Role: Co-Principal Investigator

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Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. O'Brien JJ, O'Connell JD, Paulo JA, Thakurta S, Rose CM, Weekes MP, Huttlin EL, Gygi SP. Compositional Proteomics: Effects of Spatial Constraints on Protein Quantification Utilizing Isobaric Tags. J Proteome Res. 2018 Jan 05; 17(1):590-599. PMID: 29195270.
    View in: PubMed
  2. Schweppe DK, Huttlin EL, Harper JW, Gygi SP. BioPlex Display: An Interactive Suite for Large-Scale AP-MS Protein-Protein Interaction Data. J Proteome Res. 2018 Jan 05; 17(1):722-726. PMID: 29054129.
    View in: PubMed
  3. Pontano Vaites L, Paulo JA, Huttlin EL, Harper JW. Systematic analysis of human cells lacking ATG8 proteins uncovers roles for GABARAPs and the CCZ1/MON1 regulator C18orf8/RMC1 in macro and selective autophagic flux. Mol Cell Biol. 2017 Oct 16. PMID: 29038162.
    View in: PubMed
  4. Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW. Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 05 25; 545(7655):505-509. PMID: 28514442.
    View in: PubMed
  5. Anikster Y, Haack TB, Vilboux T, Pode-Shakked B, Thöny B, Shen N, Guarani V, Meissner T, Mayatepek E, Trefz FK, Marek-Yagel D, Martinez A, Huttlin EL, Paulo JA, Berutti R, Benoist JF, Imbard A, Dorboz I, Heimer G, Landau Y, Ziv-Strasser L, Malicdan MCV, Gemperle-Britschgi C, Cremer K, Engels H, Meili D, Keller I, Bruggmann R, Strom TM, Meitinger T, Mullikin JC, Schwartz G, Ben-Zeev B, Gahl WA, Harper JW, Blau N, Hoffmann GF, Prokisch H, Opladen T, Schiff M. Biallelic Mutations in DNAJC12 Cause Hyperphenylalaninemia, Dystonia, and Intellectual Disability. Am J Hum Genet. 2017 Feb 02; 100(2):257-266. PMID: 28132689.
    View in: PubMed
  6. Everley RA, Huttlin EL, Erickson AR, Beausoleil SA, Gygi SP. Neutral Loss Is a Very Common Occurrence in Phosphotyrosine-Containing Peptides Labeled with Isobaric Tags. J Proteome Res. 2017 Feb 03; 16(2):1069-1076. PMID: 27978624.
    View in: PubMed
  7. Chick JM, Munger SC, Simecek P, Huttlin EL, Choi K, Gatti DM, Raghupathy N, Svenson KL, Churchill GA, Gygi SP. Defining the consequences of genetic variation on a proteome-wide scale. Nature. 2016 06 23; 534(7608):500-5. PMID: 27309819.
    View in: PubMed
  8. Raman M, Sergeev M, Garnaas M, Lydeard JR, Huttlin EL, Goessling W, Shah JV, Harper JW. Corrigendum: Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Nat Cell Biol. 2016 Mar; 18(3):347. PMID: 26911913.
    View in: PubMed
  9. Raman M, Sergeev M, Garnaas M, Lydeard JR, Huttlin EL, Goessling W, Shah JV, Harper JW. Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Nat Cell Biol. 2015 Oct; 17(10):1356-69. PMID: 26389662; PMCID: PMC4610257.
  10. Chick JM, Kolippakkam D, Nusinow DP, Zhai B, Rad R, Huttlin EL, Gygi SP. Erratum: A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides. Nat Biotechnol. 2015 Aug; 33(8):882. PMID: 26252148.
    View in: PubMed
  11. Huttlin EL, Ting L, Bruckner RJ, Gebreab F, Gygi MP, Szpyt J, Tam S, Zarraga G, Colby G, Baltier K, Dong R, Guarani V, Vaites LP, Ordureau A, Rad R, Erickson BK, Wühr M, Chick J, Zhai B, Kolippakkam D, Mintseris J, Obar RA, Harris T, Artavanis-Tsakonas S, Sowa ME, De Camilli P, Paulo JA, Harper JW, Gygi SP. The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16; 162(2):425-440. PMID: 26186194; PMCID: PMC4617211.
  12. Chick JM, Kolippakkam D, Nusinow DP, Zhai B, Rad R, Huttlin EL, Gygi SP. A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides. Nat Biotechnol. 2015 Jul; 33(7):743-9. PMID: 26076430; PMCID: PMC4515955.
  13. Elia AE, Boardman AP, Wang DC, Huttlin EL, Everley RA, Dephoure N, Zhou C, Koren I, Gygi SP, Elledge SJ. Quantitative Proteomic Atlas of Ubiquitination and Acetylation in the DNA Damage Response. Mol Cell. 2015 Sep 03; 59(5):867-81. PMID: 26051181; PMCID: PMC4560960.
  14. Guarani V, McNeill EM, Paulo JA, Huttlin EL, Fröhlich F, Gygi SP, Van Vactor D, Harper JW. QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. Elife. 2015 May 21; 4. PMID: 25997101; PMCID: PMC4439739.
  15. McAlister GC, Nusinow DP, Jedrychowski MP, Wühr M, Huttlin EL, Erickson BK, Rad R, Haas W, Gygi SP. MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem. 2014 Jul 15; 86(14):7150-8. PMID: 24927332; PMCID: PMC4215866.
  16. Weekes MP, Tomasec P, Huttlin EL, Fielding CA, Nusinow D, Stanton RJ, Wang EC, Aicheler R, Murrell I, Wilkinson GW, Lehner PJ, Gygi SP. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell. 2014 Jun 05; 157(6):1460-72. PMID: 24906157; PMCID: PMC4048463.
  17. Tcherkezian J, Cargnello M, Romeo Y, Huttlin EL, Lavoie G, Gygi SP, Roux PP. Proteomic analysis of cap-dependent translation identifies LARP1 as a key regulator of 5'TOP mRNA translation. Genes Dev. 2014 Feb 15; 28(4):357-71. PMID: 24532714; PMCID: PMC3937514.
  18. Guarani V, Paulo J, Zhai B, Huttlin EL, Gygi SP, Harper JW. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol. 2014 Mar; 34(5):847-61. PMID: 24344204; PMCID: PMC4023825.
  19. Pease BN, Huttlin EL, Jedrychowski MP, Talevich E, Harmon J, Dillman T, Kannan N, Doerig C, Chakrabarti R, Gygi SP, Chakrabarti D. Global analysis of protein expression and phosphorylation of three stages of Plasmodium falciparum intraerythrocytic development. J Proteome Res. 2013 Sep 06; 12(9):4028-45. PMID: 23914800.
    View in: PubMed
  20. Ivancic MM, Huttlin EL, Chen X, Pleiman JK, Irving AA, Hegeman AD, Dove WF, Sussman MR. Candidate serum biomarkers for early intestinal cancer using 15N metabolic labeling and quantitative proteomics in the ApcMin/+ mouse. J Proteome Res. 2013 Sep 06; 12(9):4152-66. PMID: 23924158; PMCID: PMC3792563.
  21. McAllister FE, Niepel M, Haas W, Huttlin E, Sorger PK, Gygi SP. Mass spectrometry based method to increase throughput for kinome analyses using ATP probes. Anal Chem. 2013 May 07; 85(9):4666-74. PMID: 23607489; PMCID: PMC3771683.
  22. Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW. Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Nature. 2013 Apr 18; 496(7445):372-6. PMID: 23503661; PMCID: PMC3641819.
  23. Zhang X, Lavoie G, Fort L, Huttlin EL, Tcherkezian J, Galan JA, Gu H, Gygi SP, Carreno S, Roux PP. Gab2 phosphorylation by RSK inhibits Shp2 recruitment and cell motility. Mol Cell Biol. 2013 Apr; 33(8):1657-70. PMID: 23401857; PMCID: PMC3624252.
  24. Cargnello M, Tcherkezian J, Dorn JF, Huttlin EL, Maddox PS, Gygi SP, Roux PP. Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly. Mol Cell Biol. 2012 Nov; 32(22):4572-84. PMID: 22966201; PMCID: PMC3486182.
  25. McAlister GC, Huttlin EL, Haas W, Ting L, Jedrychowski MP, Rogers JC, Kuhn K, Pike I, Grothe RA, Blethrow JD, Gygi SP. Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses. Anal Chem. 2012 Sep 04; 84(17):7469-78. PMID: 22880955; PMCID: PMC3715028.
  26. Jedrychowski MP, Huttlin EL, Haas W, Sowa ME, Rad R, Gygi SP. Evaluation of HCD- and CID-type fragmentation within their respective detection platforms for murine phosphoproteomics. Mol Cell Proteomics. 2011 Dec; 10(12):M111.009910. PMID: 21917720; PMCID: PMC3237076.
  27. Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP. Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 2011 Oct 21; 44(2):325-40. PMID: 21906983; PMCID: PMC3200427.
  28. Wu R, Haas W, Dephoure N, Huttlin EL, Zhai B, Sowa ME, Gygi SP. A large-scale method to measure absolute protein phosphorylation stoichiometries. Nat Methods. 2011 Jul 03; 8(8):677-83. PMID: 21725298; PMCID: PMC3146562.
  29. Wu R, Dephoure N, Haas W, Huttlin EL, Zhai B, Sowa ME, Gygi SP. Correct interpretation of comprehensive phosphorylation dynamics requires normalization by protein expression changes. Mol Cell Proteomics. 2011 Aug; 10(8):M111.009654. PMID: 21551504; PMCID: PMC3149096.
  30. Huttlin EL, Jedrychowski MP, Elias JE, Goswami T, Rad R, Beausoleil SA, Villén J, Haas W, Sowa ME, Gygi SP. A tissue-specific atlas of mouse protein phosphorylation and expression. Cell. 2010 Dec 23; 143(7):1174-89. PMID: 21183079; PMCID: PMC3035969.
  31. Huttlin EL, Chen X, Barrett-Wilt GA, Hegeman AD, Halberg RB, Harms AC, Newton MA, Dove WF, Sussman MR. Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution. Proc Natl Acad Sci U S A. 2009 Oct 06; 106(40):17235-40. PMID: 19805096; PMCID: PMC2761368.
  32. Sussman MR, Huttlin EL, Wohlbach DJ. Democratization and integration of genomic profiling tools. Methods Mol Biol. 2009; 553:373-93. PMID: 19588117.
    View in: PubMed
  33. McIlwain S, Page D, Huttlin EL, Sussman MR. Matching isotopic distributions from metabolically labeled samples. Bioinformatics. 2008 Jul 01; 24(13):i339-47. PMID: 18586733; PMCID: PMC2718665.
  34. Mock T, Samanta MP, Iverson V, Berthiaume C, Robison M, Holtermann K, Durkin C, Bondurant SS, Richmond K, Rodesch M, Kallas T, Huttlin EL, Cerrina F, Sussman MR, Armbrust EV. Whole-genome expression profiling of the marine diatom Thalassiosira pseudonana identifies genes involved in silicon bioprocesses. Proc Natl Acad Sci U S A. 2008 Feb 05; 105(5):1579-84. PMID: 18212125; PMCID: PMC2234187.
  35. Hegeman AD, Schulte CF, Cui Q, Lewis IA, Huttlin EL, Eghbalnia H, Harms AC, Ulrich EL, Markley JL, Sussman MR. Stable isotope assisted assignment of elemental compositions for metabolomics. Anal Chem. 2007 Sep 15; 79(18):6912-21. PMID: 17708672.
    View in: PubMed
  36. McIlwain S, Page D, Huttlin EL, Sussman MR. Using dynamic programming to create isotopic distribution maps from mass spectra. Bioinformatics. 2007 Jul 01; 23(13):i328-36. PMID: 17646314.
    View in: PubMed
  37. Nelson CJ, Huttlin EL, Hegeman AD, Harms AC, Sussman MR. Implications of 15N-metabolic labeling for automated peptide identification in Arabidopsis thaliana. Proteomics. 2007 Apr; 7(8):1279-92. PMID: 17443642.
    View in: PubMed
  38. Huttlin EL, Hegeman AD, Harms AC, Sussman MR. Comparison of full versus partial metabolic labeling for quantitative proteomics analysis in Arabidopsis thaliana. Mol Cell Proteomics. 2007 May; 6(5):860-81. PMID: 17293592.
    View in: PubMed
  39. Huttlin EL, Hegeman AD, Harms AC, Sussman MR. Prediction of error associated with false-positive rate determination for peptide identification in large-scale proteomics experiments using a combined reverse and forward peptide sequence database strategy. J Proteome Res. 2007 Jan; 6(1):392-8. PMID: 17203984; PMCID: PMC2572755.
  40. Pischke MS, Huttlin EL, Hegeman AD, Sussman MR. A transcriptome-based characterization of habituation in plant tissue culture. Plant Physiol. 2006 Apr; 140(4):1255-78. PMID: 16489130; PMCID: PMC1435795.
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