Harvard Catalyst Profiles

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Edward Leo Huttlin, Ph.D.


The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. U24HG006673 (GYGI, STEVEN P) Aug 21, 2012 - Jun 30, 2022
    Systematic Exploration of the Human Interactome
    Role: Co-Principal Investigator
  2. U41HG006673 (GYGI, STEVEN P) Aug 21, 2012 - Jun 30, 2018
    Systematic Exploration of the Human Interactome
    Role: Co-Principal Investigator

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Qin Y, Huttlin EL, Winsnes CF, Gosztyla ML, Wacheul L, Kelly MR, Blue SM, Zheng F, Chen M, Schaffer LV, Licon K, Bäckström A, Vaites LP, Lee JJ, Ouyang W, Liu SN, Zhang T, Silva E, Park J, Pitea A, Kreisberg JF, Gygi SP, Ma J, Harper JW, Yeo GW, Lafontaine DLJ, Lundberg E, Ideker T. A multi-scale map of cell structure fusing protein images and interactions. Nature. 2021 Dec; 600(7889):536-542. PMID: 34819669.
    Citations: 1     Fields:    
  2. Li J, Cai Z, Vaites LP, Shen N, Mitchell DC, Huttlin EL, Paulo JA, Harry BL, Gygi SP. Proteome-wide mapping of short-lived proteins in human cells. Mol Cell. 2021 11 18; 81(22):4722-4735.e5. PMID: 34626566.
    Citations:    Fields:    Translation:HumansCells
  3. Huttlin EL, Bruckner RJ, Navarrete-Perea J, Cannon JR, Baltier K, Gebreab F, Gygi MP, Thornock A, Zarraga G, Tam S, Szpyt J, Gassaway BM, Panov A, Parzen H, Fu S, Golbazi A, Maenpaa E, Stricker K, Guha Thakurta S, Zhang T, Rad R, Pan J, Nusinow DP, Paulo JA, Schweppe DK, Vaites LP, Harper JW, Gygi SP. Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell. 2021 05 27; 184(11):3022-3040.e28. PMID: 33961781.
    Citations: 44     Fields:    Translation:HumansCells
  4. Li J, Van Vranken JG, Paulo JA, Huttlin EL, Gygi SP. Selection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay. J Proteome Res. 2020 05 01; 19(5):2159-2166. PMID: 32243163.
    Citations: 2     Fields:    
  5. Schweppe DK, Eng JK, Yu Q, Bailey D, Rad R, Navarrete-Perea J, Huttlin EL, Erickson BK, Paulo JA, Gygi SP. Full-Featured, Real-Time Database Searching Platform Enables Fast and Accurate Multiplexed Quantitative Proteomics. J Proteome Res. 2020 05 01; 19(5):2026-2034. PMID: 32126768.
    Citations: 36     Fields:    
  6. Li J, Van Vranken JG, Pontano Vaites L, Schweppe DK, Huttlin EL, Etienne C, Nandhikonda P, Viner R, Robitaille AM, Thompson AH, Kuhn K, Pike I, Bomgarden RD, Rogers JC, Gygi SP, Paulo JA. TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples. Nat Methods. 2020 04; 17(4):399-404. PMID: 32203386.
    Citations: 59     Fields:    Translation:HumansCells
  7. Xiao H, Jedrychowski MP, Schweppe DK, Huttlin EL, Yu Q, Heppner DE, Li J, Long J, Mills EL, Szpyt J, He Z, Du G, Garrity R, Reddy A, Vaites LP, Paulo JA, Zhang T, Gray NS, Gygi SP, Chouchani ET. A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging. Cell. 2020 03 05; 180(5):968-983.e24. PMID: 32109415.
    Citations: 54     Fields:    Translation:HumansAnimalsCells
  8. Nobre LV, Nightingale K, Ravenhill BJ, Antrobus R, Soday L, Nichols J, Davies JA, Seirafian S, Wang EC, Davison AJ, Wilkinson GW, Stanton RJ, Huttlin EL, Weekes MP. Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. Elife. 2019 12 24; 8. PMID: 31873071.
    Citations: 25     Fields:    Translation:HumansCells
  9. Li J, Paulo JA, Nusinow DP, Huttlin EL, Gygi SP. Investigation of Proteomic and Phosphoproteomic Responses to Signaling Network Perturbations Reveals Functional Pathway Organizations in Yeast. Cell Rep. 2019 11 12; 29(7):2092-2104.e4. PMID: 31722220.
    Citations: 10     Fields:    Translation:AnimalsCells
  10. Nightingale K, Lin KM, Ravenhill BJ, Davies C, Nobre L, Fielding CA, Ruckova E, Fletcher-Etherington A, Soday L, Nichols H, Sugrue D, Wang ECY, Moreno P, Umrania Y, Huttlin EL, Antrobus R, Davison AJ, Wilkinson GWG, Stanton RJ, Tomasec P, Weekes MP. High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms. Cell Host Microbe. 2018 09 12; 24(3):447-460.e11. PMID: 30122656.
    Citations: 37     Fields:    Translation:HumansCells
  11. Pease BN, Huttlin EL, Jedrychowski MP, Dorin-Semblat D, Sebastiani D, Segarra DT, Roberts BF, Chakrabarti R, Doerig C, Gygi SP, Chakrabarti D. Characterization of Plasmodium falciparum Atypical Kinase PfPK7- Dependent Phosphoproteome. J Proteome Res. 2018 06 01; 17(6):2112-2123. PMID: 29678115.
    Citations: 9     Fields:    Translation:HumansAnimalsCells
  12. Bouchereau J, Huttlin EL, Guarani V, Pichard S, Anikster Y, Schiff M. DNAJC12: A molecular chaperone involved in proteostasis, PKU, biogenic amines metabolism and beyond? Mol Genet Metab. 2018 03; 123(3):285-286. PMID: 29396030.
    Citations:    Fields:    Translation:HumansCells
  13. O'Brien JJ, O'Connell JD, Paulo JA, Thakurta S, Rose CM, Weekes MP, Huttlin EL, Gygi SP. Compositional Proteomics: Effects of Spatial Constraints on Protein Quantification Utilizing Isobaric Tags. J Proteome Res. 2018 01 05; 17(1):590-599. PMID: 29195270.
    Citations: 22     Fields:    
  14. Vaites LP, Paulo JA, Huttlin EL, Harper JW. Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux. Mol Cell Biol. 2018 01 01; 38(1). PMID: 29038162.
    Citations: 40     Fields:    Translation:HumansCells
  15. Schweppe DK, Huttlin EL, Harper JW, Gygi SP. BioPlex Display: An Interactive Suite for Large-Scale AP-MS Protein-Protein Interaction Data. J Proteome Res. 2018 01 05; 17(1):722-726. PMID: 29054129.
    Citations: 16     Fields:    Translation:Humans
  16. Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW. Architecture of the human interactome defines protein communities and disease networks. Nature. 2017 05 25; 545(7655):505-509. PMID: 28514442.
    Citations: 482     Fields:    Translation:HumansCells
  17. Anikster Y, Haack TB, Vilboux T, Pode-Shakked B, Thöny B, Shen N, Guarani V, Meissner T, Mayatepek E, Trefz FK, Marek-Yagel D, Martinez A, Huttlin EL, Paulo JA, Berutti R, Benoist JF, Imbard A, Dorboz I, Heimer G, Landau Y, Ziv-Strasser L, Malicdan MCV, Gemperle-Britschgi C, Cremer K, Engels H, Meili D, Keller I, Bruggmann R, Strom TM, Meitinger T, Mullikin JC, Schwartz G, Ben-Zeev B, Gahl WA, Harper JW, Blau N, Hoffmann GF, Prokisch H, Opladen T, Schiff M. Biallelic Mutations in DNAJC12 Cause Hyperphenylalaninemia, Dystonia, and Intellectual Disability. Am J Hum Genet. 2017 02 02; 100(2):257-266. PMID: 28132689.
    Citations: 40     Fields:    Translation:HumansCells
  18. Everley RA, Huttlin EL, Erickson AR, Beausoleil SA, Gygi SP. Neutral Loss Is a Very Common Occurrence in Phosphotyrosine-Containing Peptides Labeled with Isobaric Tags. J Proteome Res. 2017 02 03; 16(2):1069-1076. PMID: 27978624.
    Citations: 3     Fields:    
  19. Chick JM, Munger SC, Simecek P, Huttlin EL, Choi K, Gatti DM, Raghupathy N, Svenson KL, Churchill GA, Gygi SP. Defining the consequences of genetic variation on a proteome-wide scale. Nature. 2016 06 23; 534(7608):500-5. PMID: 27309819.
    Citations: 137     Fields:    Translation:AnimalsCells
  20. Raman M, Sergeev M, Garnaas M, Lydeard JR, Huttlin EL, Goessling W, Shah JV, Harper JW. Corrigendum: Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Nat Cell Biol. 2016 Mar; 18(3):347. PMID: 26911913.
    Citations:    Fields:    
  21. Raman M, Sergeev M, Garnaas M, Lydeard JR, Huttlin EL, Goessling W, Shah JV, Harper JW. Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Nat Cell Biol. 2015 Oct; 17(10):1356-69. PMID: 26389662.
    Citations: 37     Fields:    Translation:HumansAnimalsCells
  22. Chick JM, Kolippakkam D, Nusinow DP, Zhai B, Rad R, Huttlin EL, Gygi SP. Erratum: A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides. Nat Biotechnol. 2015 Aug; 33(8):882. PMID: 26252148.
    Citations: 2     Fields:    
  23. Huttlin EL, Ting L, Bruckner RJ, Gebreab F, Gygi MP, Szpyt J, Tam S, Zarraga G, Colby G, Baltier K, Dong R, Guarani V, Vaites LP, Ordureau A, Rad R, Erickson BK, Wühr M, Chick J, Zhai B, Kolippakkam D, Mintseris J, Obar RA, Harris T, Artavanis-Tsakonas S, Sowa ME, De Camilli P, Paulo JA, Harper JW, Gygi SP. The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell. 2015 Jul 16; 162(2):425-440. PMID: 26186194.
    Citations: 585     Fields:    Translation:Humans
  24. Chick JM, Kolippakkam D, Nusinow DP, Zhai B, Rad R, Huttlin EL, Gygi SP. A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides. Nat Biotechnol. 2015 Jul; 33(7):743-9. PMID: 26076430.
    Citations: 100     Fields:    Translation:HumansCells
  25. Elia AE, Boardman AP, Wang DC, Huttlin EL, Everley RA, Dephoure N, Zhou C, Koren I, Gygi SP, Elledge SJ. Quantitative Proteomic Atlas of Ubiquitination and Acetylation in the DNA Damage Response. Mol Cell. 2015 Sep 03; 59(5):867-81. PMID: 26051181.
    Citations: 129     Fields:    Translation:HumansCells
  26. Guarani V, McNeill EM, Paulo JA, Huttlin EL, Fröhlich F, Gygi SP, Van Vactor D, Harper JW. QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. Elife. 2015 May 21; 4. PMID: 25997101.
    Citations: 65     Fields:    Translation:HumansAnimalsCells
  27. McAlister GC, Nusinow DP, Jedrychowski MP, Wühr M, Huttlin EL, Erickson BK, Rad R, Haas W, Gygi SP. MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across cancer cell line proteomes. Anal Chem. 2014 Jul 15; 86(14):7150-8. PMID: 24927332.
    Citations: 460     Fields:    Translation:HumansCells
  28. Weekes MP, Tomasec P, Huttlin EL, Fielding CA, Nusinow D, Stanton RJ, Wang ECY, Aicheler R, Murrell I, Wilkinson GWG, Lehner PJ, Gygi SP. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell. 2014 Jun 05; 157(6):1460-1472. PMID: 24906157.
    Citations: 217     Fields:    Translation:HumansCells
  29. Tcherkezian J, Cargnello M, Romeo Y, Huttlin EL, Lavoie G, Gygi SP, Roux PP. Proteomic analysis of cap-dependent translation identifies LARP1 as a key regulator of 5'TOP mRNA translation. Genes Dev. 2014 Feb 15; 28(4):357-71. PMID: 24532714.
    Citations: 118     Fields:    Translation:HumansAnimalsCells
  30. Guarani V, Paulo J, Zhai B, Huttlin EL, Gygi SP, Harper JW. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol. 2014 Mar; 34(5):847-61. PMID: 24344204.
    Citations: 42     Fields:    Translation:HumansCells
  31. Pease BN, Huttlin EL, Jedrychowski MP, Talevich E, Harmon J, Dillman T, Kannan N, Doerig C, Chakrabarti R, Gygi SP, Chakrabarti D. Global analysis of protein expression and phosphorylation of three stages of Plasmodium falciparum intraerythrocytic development. J Proteome Res. 2013 Sep 06; 12(9):4028-45. PMID: 23914800.
    Citations: 69     Fields:    Translation:HumansAnimalsCells
  32. Ivancic MM, Huttlin EL, Chen X, Pleiman JK, Irving AA, Hegeman AD, Dove WF, Sussman MR. Candidate serum biomarkers for early intestinal cancer using 15N metabolic labeling and quantitative proteomics in the ApcMin/+ mouse. J Proteome Res. 2013 Sep 06; 12(9):4152-66. PMID: 23924158.
    Citations: 13     Fields:    Translation:HumansAnimalsCells
  33. McAllister FE, Niepel M, Haas W, Huttlin E, Sorger PK, Gygi SP. Mass spectrometry based method to increase throughput for kinome analyses using ATP probes. Anal Chem. 2013 May 07; 85(9):4666-74. PMID: 23607489.
    Citations: 14     Fields:    Translation:HumansCells
  34. Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW. Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Nature. 2013 Apr 18; 496(7445):372-6. PMID: 23503661.
    Citations: 454     Fields:    Translation:HumansAnimalsCells
  35. Zhang X, Lavoie G, Fort L, Huttlin EL, Tcherkezian J, Galan JA, Gu H, Gygi SP, Carreno S, Roux PP. Gab2 phosphorylation by RSK inhibits Shp2 recruitment and cell motility. Mol Cell Biol. 2013 Apr; 33(8):1657-70. PMID: 23401857.
    Citations: 12     Fields:    Translation:HumansAnimalsCells
  36. Cargnello M, Tcherkezian J, Dorn JF, Huttlin EL, Maddox PS, Gygi SP, Roux PP. Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly. Mol Cell Biol. 2012 Nov; 32(22):4572-84. PMID: 22966201.
    Citations: 19     Fields:    Translation:HumansCells
  37. McAlister GC, Huttlin EL, Haas W, Ting L, Jedrychowski MP, Rogers JC, Kuhn K, Pike I, Grothe RA, Blethrow JD, Gygi SP. Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses. Anal Chem. 2012 Sep 04; 84(17):7469-78. PMID: 22880955.
    Citations: 245     Fields:    Translation:HumansAnimalsCells
  38. Jedrychowski MP, Huttlin EL, Haas W, Sowa ME, Rad R, Gygi SP. Evaluation of HCD- and CID-type fragmentation within their respective detection platforms for murine phosphoproteomics. Mol Cell Proteomics. 2011 Dec; 10(12):M111.009910. PMID: 21917720.
    Citations: 66     Fields:    Translation:AnimalsCells
  39. Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP. Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 2011 Oct 21; 44(2):325-40. PMID: 21906983.
    Citations: 756     Fields:    Translation:HumansCells
  40. Wu R, Haas W, Dephoure N, Huttlin EL, Zhai B, Sowa ME, Gygi SP. A large-scale method to measure absolute protein phosphorylation stoichiometries. Nat Methods. 2011 Jul 03; 8(8):677-83. PMID: 21725298.
    Citations: 131     Fields:    Translation:Cells
  41. Wu R, Dephoure N, Haas W, Huttlin EL, Zhai B, Sowa ME, Gygi SP. Correct interpretation of comprehensive phosphorylation dynamics requires normalization by protein expression changes. Mol Cell Proteomics. 2011 Aug; 10(8):M111.009654. PMID: 21551504.
    Citations: 79     Fields:    Translation:AnimalsCells
  42. Huttlin EL, Jedrychowski MP, Elias JE, Goswami T, Rad R, Beausoleil SA, Villén J, Haas W, Sowa ME, Gygi SP. A tissue-specific atlas of mouse protein phosphorylation and expression. Cell. 2010 Dec 23; 143(7):1174-89. PMID: 21183079.
    Citations: 810     Fields:    Translation:AnimalsCells
  43. Huttlin EL, Chen X, Barrett-Wilt GA, Hegeman AD, Halberg RB, Harms AC, Newton MA, Dove WF, Sussman MR. Discovery and validation of colonic tumor-associated proteins via metabolic labeling and stable isotopic dilution. Proc Natl Acad Sci U S A. 2009 Oct 06; 106(40):17235-40. PMID: 19805096.
    Citations: 11     Fields:    Translation:HumansAnimalsCells
  44. Sussman MR, Huttlin EL, Wohlbach DJ. Democratization and integration of genomic profiling tools. Methods Mol Biol. 2009; 553:373-93. PMID: 19588117.
    Citations:    Fields:    Translation:Animals
  45. McIlwain S, Page D, Huttlin EL, Sussman MR. Matching isotopic distributions from metabolically labeled samples. Bioinformatics. 2008 Jul 01; 24(13):i339-47. PMID: 18586733.
    Citations: 2     Fields:    Translation:Cells
  46. Mock T, Samanta MP, Iverson V, Berthiaume C, Robison M, Holtermann K, Durkin C, Bondurant SS, Richmond K, Rodesch M, Kallas T, Huttlin EL, Cerrina F, Sussman MR, Armbrust EV. Whole-genome expression profiling of the marine diatom Thalassiosira pseudonana identifies genes involved in silicon bioprocesses. Proc Natl Acad Sci U S A. 2008 Feb 05; 105(5):1579-84. PMID: 18212125.
    Citations: 78     Fields:    Translation:Animals
  47. Hegeman AD, Schulte CF, Cui Q, Lewis IA, Huttlin EL, Eghbalnia H, Harms AC, Ulrich EL, Markley JL, Sussman MR. Stable isotope assisted assignment of elemental compositions for metabolomics. Anal Chem. 2007 Sep 15; 79(18):6912-21. PMID: 17708672.
    Citations: 33     Fields:    Translation:AnimalsCells
  48. McIlwain S, Page D, Huttlin EL, Sussman MR. Using dynamic programming to create isotopic distribution maps from mass spectra. Bioinformatics. 2007 Jul 01; 23(13):i328-36. PMID: 17646314.
    Citations: 8     Fields:    
  49. Nelson CJ, Huttlin EL, Hegeman AD, Harms AC, Sussman MR. Implications of 15N-metabolic labeling for automated peptide identification in Arabidopsis thaliana. Proteomics. 2007 Apr; 7(8):1279-92. PMID: 17443642.
    Citations: 30     Fields:    Translation:Animals
  50. Huttlin EL, Hegeman AD, Harms AC, Sussman MR. Comparison of full versus partial metabolic labeling for quantitative proteomics analysis in Arabidopsis thaliana. Mol Cell Proteomics. 2007 May; 6(5):860-81. PMID: 17293592.
    Citations: 28     Fields:    Translation:AnimalsCells
  51. Huttlin EL, Hegeman AD, Harms AC, Sussman MR. Prediction of error associated with false-positive rate determination for peptide identification in large-scale proteomics experiments using a combined reverse and forward peptide sequence database strategy. J Proteome Res. 2007 Jan; 6(1):392-8. PMID: 17203984.
    Citations: 29     Fields:    Translation:HumansAnimals
  52. Pischke MS, Huttlin EL, Hegeman AD, Sussman MR. A transcriptome-based characterization of habituation in plant tissue culture. Plant Physiol. 2006 Apr; 140(4):1255-78. PMID: 16489130.
    Citations: 24     Fields:    Translation:AnimalsCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.