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Last Name

profileStephen Buratowski, PH.D.

TitleProfessor of Biological Chemistry and Molecular Pharmacology
InstitutionHarvard Medical School
DepartmentBiological Chemistry & Molecular Pharmacology
AddressHarvard Medical School
Dept. BCMP C1-210
240 Longwood Ave
Boston MA 02115
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The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
R01GM056663     (BURATOWSKI, STEPHEN)Jul 1, 1999 - Jun 30, 2019
mRNA Capping Enzyme
Role: Principal Investigator

R01GM046498     (BURATOWSKI, STEPHEN)Jul 6, 1991 - Mar 31, 2021
The RNA polymerase II transcription complex
Role: Principal Investigator

R29GM046498     (BURATOWSKI, STEPHEN)Jul 6, 1991 - Jun 30, 1996
Role: Principal Investigator

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Collapse selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. du Mee DJM, Ivanov M, Parker JP, Buratowski S, Marquardt S. Efficient termination of nuclear lncRNA transcription promotes mitochondrial genome maintenance. Elife. 2018 Mar 05; 7. PMID: 29504936.
    View in: PubMed
  2. Kim JH, Lee BB, Oh YM, Zhu C, Steinmetz LM, Lee Y, Kim WK, Lee SB, Buratowski S, Kim T. Erratum: Modulation of mRNA and lncRNA expression dynamics by the Set2-Rpd3S pathway. Nat Commun. 2017 Nov 29; 8:16122. PMID: 29184145.
    View in: PubMed
  3. Soares LM, He PC, Chun Y, Suh H, Kim T, Buratowski S. Determinants of Histone H3K4 Methylation Patterns. Mol Cell. 2017 Nov 16; 68(4):773-785.e6. PMID: 29129639.
    View in: PubMed
  4. Joo YJ, Ficarro SB, Soares LM, Chun Y, Marto JA, Buratowski S. Downstream promoter interactions of TFIID TAFs facilitate transcription reinitiation. Genes Dev. 2017 11 01; 31(21):2162-2174. PMID: 29203645.
    View in: PubMed
  5. Church VA, Pressman S, Isaji M, Truscott M, Cizmecioglu NT, Buratowski S, Frolov MV, Carthew RW. Microprocessor Recruitment to Elongating RNA Polymerase II Is Required for Differential Expression of MicroRNAs. Cell Rep. 2017 Sep 26; 20(13):3123-3134. PMID: 28954229.
    View in: PubMed
  6. Woo H, Dam Ha S, Lee SB, Buratowski S, Kim T. Modulation of gene expression dynamics by co-transcriptional histone methylations. Exp Mol Med. 2017 04 28; 49(4):e326. PMID: 28450734.
    View in: PubMed
  7. Kim JH, Lee BB, Oh YM, Zhu C, Steinmetz LM, Lee Y, Kim WK, Lee SB, Buratowski S, Kim T. Modulation of mRNA and lncRNA expression dynamics by the Set2-Rpd3S pathway. Nat Commun. 2016 Nov 28; 7:13534. PMID: 27892458.
    View in: PubMed
  8. Hahn S, Buratowski S. Structural biology: Snapshots of transcription initiation. Nature. 2016 05 19; 533(7603):331-2. PMID: 27193677.
    View in: PubMed
  9. Suh H, Ficarro SB, Kang UB, Chun Y, Marto JA, Buratowski S. Direct Analysis of Phosphorylation Sites on the Rpb1 C-Terminal Domain of RNA Polymerase II. Mol Cell. 2016 Jan 21; 61(2):297-304. PMID: 26799764; PMCID: PMC4724063 [Available on 01/21/17].
  10. Marquardt S, Escalante-Chong R, Pho N, Wang J, Churchman LS, Springer M, Buratowski S. A Chromatin-Based Mechanism for Limiting Divergent Noncoding Transcription. Cell. 2014 Jul 17; 158(2):462. PMID: 28915368.
    View in: PubMed
  11. Marquardt S, Escalante-Chong R, Pho N, Wang J, Churchman LS, Springer M, Buratowski S. A chromatin-based mechanism for limiting divergent noncoding transcription. Cell. 2014 Jun 19; 157(7):1712-23. PMID: 24949978; PMCID: PMC4090027.
  12. Soares LM, Radman-Livaja M, Lin SG, Rando OJ, Buratowski S. Feedback control of Set1 protein levels is important for proper H3K4 methylation patterns. Cell Rep. 2014 Mar 27; 6(6):961-972. PMID: 24613354; PMCID: PMC3999964.
  13. Heo DH, Yoo I, Kong J, Lidschreiber M, Mayer A, Choi BY, Hahn Y, Cramer P, Buratowski S, Kim M. The RNA polymerase II C-terminal domain-interacting domain of yeast Nrd1 contributes to the choice of termination pathway and couples to RNA processing by the nuclear exosome. J Biol Chem. 2013 Dec 20; 288(51):36676-90. PMID: 24196955; PMCID: PMC3868778.
  14. Suh H, Hazelbaker DZ, Soares LM, Buratowski S. The C-terminal domain of Rpb1 functions on other RNA polymerase II subunits. Mol Cell. 2013 Sep 26; 51(6):850-8. PMID: 24035501; PMCID: PMC3819399.
  15. Soares LM, Buratowski S. Histone Crosstalk: H2Bub and H3K4 Methylation. Mol Cell. 2013 Mar 28; 49(6):1019-20. PMID: 23541037; PMCID: PMC3753804.
  16. Fowler T, Suh H, Buratowski S, Roy AL. Regulation of primary response genes in B cells. J Biol Chem. 2013 May 24; 288(21):14906-16. PMID: 23536186; PMCID: PMC3663512.
  17. Hazelbaker DZ, Marquardt S, Wlotzka W, Buratowski S. Kinetic competition between RNA Polymerase II and Sen1-dependent transcription termination. Mol Cell. 2013 Jan 10; 49(1):55-66. PMID: 23177741; PMCID: PMC3545030.
  18. Hazelbaker DZ, Buratowski S. Transcription: base J blocks the way. Curr Biol. 2012 Nov 20; 22(22):R960-2. PMID: 23174300; PMCID: PMC3648658.
  19. van Werven FJ, Neuert G, Hendrick N, Lardenois A, Buratowski S, van Oudenaarden A, Primig M, Amon A. Transcription of two long noncoding RNAs mediates mating-type control of gametogenesis in budding yeast. Cell. 2012 Sep 14; 150(6):1170-81. PMID: 22959267; PMCID: PMC3472370.
  20. Kim T, Xu Z, Clauder-Münster S, Steinmetz LM, Buratowski S. Set3 HDAC mediates effects of overlapping noncoding transcription on gene induction kinetics. Cell. 2012 Sep 14; 150(6):1158-69. PMID: 22959268; PMCID: PMC3461055.
  21. Sikorski TW, Joo YJ, Ficarro SB, Askenazi M, Buratowski S, Marto JA. Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters. J Biol Chem. 2012 Oct 12; 287(42):35397-408. PMID: 22902623; PMCID: PMC3471693.
  22. Weiner A, Chen HV, Liu CL, Rahat A, Klien A, Soares L, Gudipati M, Pfeffner J, Regev A, Buratowski S, Pleiss JA, Friedman N, Rando OJ. Systematic dissection of roles for chromatin regulators in a yeast stress response. PLoS Biol. 2012; 10(7):e1001369. PMID: 22912562; PMCID: PMC3416867.
  23. Radman-Livaja M, Quan TK, Valenzuela L, Armstrong JA, van Welsem T, Kim T, Lee LJ, Buratowski S, van Leeuwen F, Rando OJ, Hartzog GA. A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast. PLoS Genet. 2012; 8(7):e1002811. PMID: 22807688; PMCID: PMC3395613.
  24. Soares LM, Buratowski S. Yeast Swd2 is essential because of antagonism between Set1 histone methyltransferase complex and APT (associated with Pta1) termination factor. J Biol Chem. 2012 May 04; 287(19):15219-31. PMID: 22431730; PMCID: PMC3346109.
  25. Buratowski S. Gene expression: transcription initiation unwrapped. Nature. 2012 Mar 14; 483(7389):286-7. PMID: 22422261; PMCID: PMC3642986.
  26. Sikorski TW, Ficarro SB, Holik J, Kim T, Rando OJ, Marto JA, Buratowski S. Sub1 and RPA associate with RNA polymerase II at different stages of transcription. Mol Cell. 2011 Nov 04; 44(3):397-409. PMID: 22055186; PMCID: PMC3227220.
  27. Terzi N, Churchman LS, Vasiljeva L, Weissman J, Buratowski S. H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway. Mol Cell Biol. 2011 Sep; 31(17):3569-83. PMID: 21709022; PMCID: PMC3165552.
  28. Lenstra TL, Benschop JJ, Kim T, Schulze JM, Brabers NA, Margaritis T, van de Pasch LA, van Heesch SA, Brok MO, Groot Koerkamp MJ, Ko CW, van Leenen D, Sameith K, van Hooff SR, Lijnzaad P, Kemmeren P, Hentrich T, Kobor MS, Buratowski S, Holstege FC. The specificity and topology of chromatin interaction pathways in yeast. Mol Cell. 2011 May 20; 42(4):536-49. PMID: 21596317; PMCID: PMC4435841.
  29. Marquardt S, Hazelbaker DZ, Buratowski S. Distinct RNA degradation pathways and 3' extensions of yeast non-coding RNA species. Transcription. 2011 May; 2(3):145-154. PMID: 21826286.
    View in: PubMed
  30. Buratowski S, Kim T. The role of cotranscriptional histone methylations. Cold Spring Harb Symp Quant Biol. 2010; 75:95-102. PMID: 21447819; PMCID: PMC3229092.
  31. Lunde BM, Reichow SL, Kim M, Suh H, Leeper TC, Yang F, Mutschler H, Buratowski S, Meinhart A, Varani G. Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain. Nat Struct Mol Biol. 2010 Oct; 17(10):1195-201. PMID: 20818393; PMCID: PMC2950884.
  32. Dermody JL, Buratowski S. Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment. J Biol Chem. 2010 Oct 29; 285(44):33671-9. PMID: 20732871; PMCID: PMC2962465.
  33. Kim TS, Liu CL, Yassour M, Holik J, Friedman N, Buratowski S, Rando OJ. RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast. Genome Biol. 2010; 11(7):R75. PMID: 20637075; PMCID: PMC2926786.
  34. Buratowski S. Progression through the RNA polymerase II CTD cycle. Mol Cell. 2009 Nov 25; 36(4):541-6. PMID: 19941815; PMCID: PMC3232742.
  35. Johnson A, Li G, Sikorski TW, Buratowski S, Woodcock CL, Moazed D. Reconstitution of heterochromatin-dependent transcriptional gene silencing. Mol Cell. 2009 Sep 24; 35(6):769-81. PMID: 19782027; PMCID: PMC2842978.
  36. Kim M, Suh H, Cho EJ, Buratowski S. Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7. J Biol Chem. 2009 Sep 25; 284(39):26421-6. PMID: 19679665; PMCID: PMC2785330.
  37. Sikorski TW, Buratowski S. The basal initiation machinery: beyond the general transcription factors. Curr Opin Cell Biol. 2009 Jun; 21(3):344-51. PMID: 19411170; PMCID: PMC2692371.
  38. Kim T, Buratowski S. Dimethylation of H3K4 by Set1 recruits the Set3 histone deacetylase complex to 5' transcribed regions. Cell. 2009 Apr 17; 137(2):259-72. PMID: 19379692; PMCID: PMC2802783.
  39. Ahn SH, Keogh MC, Buratowski S. Ctk1 promotes dissociation of basal transcription factors from elongating RNA polymerase II. EMBO J. 2009 Feb 04; 28(3):205-12. PMID: 19131970; PMCID: PMC2632940.
  40. Buratowski S. Transcription. Gene expression--where to start? Science. 2008 Dec 19; 322(5909):1804-5. PMID: 19095933; PMCID: PMC3516868.
  41. Dermody JL, Dreyfuss JM, Villén J, Ogundipe B, Gygi SP, Park PJ, Ponticelli AS, Moore CL, Buratowski S, Bucheli ME. Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation. PLoS One. 2008 Sep 26; 3(9):e3273. PMID: 18818768; PMCID: PMC2538588.
  42. Vasiljeva L, Kim M, Mutschler H, Buratowski S, Meinhart A. The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nat Struct Mol Biol. 2008 Aug; 15(8):795-804. PMID: 18660819; PMCID: PMC2597375.
  43. Vasiljeva L, Kim M, Terzi N, Soares LM, Buratowski S. Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin. Mol Cell. 2008 Feb 15; 29(3):313-23. PMID: 18280237.
    View in: PubMed
  44. Runner VM, Podolny V, Buratowski S. The Rpb4 subunit of RNA polymerase II contributes to cotranscriptional recruitment of 3' processing factors. Mol Cell Biol. 2008 Mar; 28(6):1883-91. PMID: 18195044; PMCID: PMC2268395.
  45. Deka P, Bucheli ME, Moore C, Buratowski S, Varani G. Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals. J Mol Biol. 2008 Jan 04; 375(1):136-50. PMID: 18022637; PMCID: PMC2214835.
  46. Bucheli ME, He X, Kaplan CD, Moore CL, Buratowski S. Polyadenylation site choice in yeast is affected by competition between Npl3 and polyadenylation factor CFI. RNA. 2007 Oct; 13(10):1756-64. PMID: 17684230; PMCID: PMC1986811.
  47. Huarte M, Lan F, Kim T, Vaughn MW, Zaratiegui M, Martienssen RA, Buratowski S, Shi Y. The fission yeast Jmj2 reverses histone H3 Lysine 4 trimethylation. J Biol Chem. 2007 Jul 27; 282(30):21662-70. PMID: 17550896.
    View in: PubMed
  48. Kim T, Buratowski S. Two Saccharomyces cerevisiae JmjC domain proteins demethylate histone H3 Lys36 in transcribed regions to promote elongation. J Biol Chem. 2007 Jul 20; 282(29):20827-35. PMID: 17525156.
    View in: PubMed
  49. Dion MF, Kaplan T, Kim M, Buratowski S, Friedman N, Rando OJ. Dynamics of replication-independent histone turnover in budding yeast. Science. 2007 Mar 09; 315(5817):1405-8. PMID: 17347438.
    View in: PubMed
  50. Kim M, Vasiljeva L, Rando OJ, Zhelkovsky A, Moore C, Buratowski S. Distinct pathways for snoRNA and mRNA termination. Mol Cell. 2006 Dec 08; 24(5):723-34. PMID: 17157255.
    View in: PubMed
  51. Voynov V, Verstrepen KJ, Jansen A, Runner VM, Buratowski S, Fink GR. Genes with internal repeats require the THO complex for transcription. Proc Natl Acad Sci U S A. 2006 Sep 26; 103(39):14423-8. PMID: 16983072; PMCID: PMC1599979.
  52. Keogh MC, Mennella TA, Sawa C, Berthelet S, Krogan NJ, Wolek A, Podolny V, Carpenter LR, Greenblatt JF, Baetz K, Buratowski S. The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4. Genes Dev. 2006 Mar 15; 20(6):660-5. PMID: 16543219; PMCID: PMC1413285.
  53. Vasiljeva L, Buratowski S. Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts. Mol Cell. 2006 Jan 20; 21(2):239-48. PMID: 16427013.
    View in: PubMed
  54. Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J. gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Mol Cell. 2005 Dec 09; 20(5):801-9. PMID: 16310392.
    View in: PubMed
  55. Keogh MC, Kim JA, Downey M, Fillingham J, Chowdhury D, Harrison JC, Onishi M, Datta N, Galicia S, Emili A, Lieberman J, Shen X, Buratowski S, Haber JE, Durocher D, Greenblatt JF, Krogan NJ. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature. 2006 Jan 26; 439(7075):497-501. PMID: 16299494.
    View in: PubMed
  56. Keogh MC, Kurdistani SK, Morris SA, Ahn SH, Podolny V, Collins SR, Schuldiner M, Chin K, Punna T, Thompson NJ, Boone C, Emili A, Weissman JS, Hughes TR, Strahl BD, Grunstein M, Greenblatt JF, Buratowski S, Krogan NJ. Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. Cell. 2005 Nov 18; 123(4):593-605. PMID: 16286008.
    View in: PubMed
  57. Liu CL, Kaplan T, Kim M, Buratowski S, Schreiber SL, Friedman N, Rando OJ. Single-nucleosome mapping of histone modifications in S. cerevisiae. PLoS Biol. 2005 Oct; 3(10):e328. PMID: 16122352; PMCID: PMC1195719.
  58. Buratowski S, Moazed D. Gene regulation: expression and silencing coupled. Nature. 2005 Jun 30; 435(7046):1174-5. PMID: 15988510.
    View in: PubMed
  59. Buratowski S. Connections between mRNA 3' end processing and transcription termination. Curr Opin Cell Biol. 2005 Jun; 17(3):257-61. PMID: 15901494.
    View in: PubMed
  60. Bucheli ME, Buratowski S. Npl3 is an antagonist of mRNA 3' end formation by RNA polymerase II. EMBO J. 2005 Jun 15; 24(12):2150-60. PMID: 15902270; PMCID: PMC1150882.
  61. Krogan NJ, Lam MH, Fillingham J, Keogh MC, Gebbia M, Li J, Datta N, Cagney G, Buratowski S, Emili A, Greenblatt JF. Proteasome involvement in the repair of DNA double-strand breaks. Mol Cell. 2004 Dec 22; 16(6):1027-34. PMID: 15610744.
    View in: PubMed
  62. Kim M, Krogan NJ, Vasiljeva L, Rando OJ, Nedea E, Greenblatt JF, Buratowski S. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature. 2004 Nov 25; 432(7016):517-22. PMID: 15565157.
    View in: PubMed
  63. Auty R, Steen H, Myers LC, Persinger J, Bartholomew B, Gygi SP, Buratowski S. Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem. 2004 Nov 26; 279(48):49973-81. PMID: 15448131.
    View in: PubMed
  64. Krogan NJ, Baetz K, Keogh MC, Datta N, Sawa C, Kwok TC, Thompson NJ, Davey MG, Pootoolal J, Hughes TR, Emili A, Buratowski S, Hieter P, Greenblatt JF. Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4. Proc Natl Acad Sci U S A. 2004 Sep 14; 101(37):13513-8. PMID: 15353583; PMCID: PMC518788.
  65. Sawa C, Nedea E, Krogan N, Wada T, Handa H, Greenblatt J, Buratowski S. Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2. Mol Cell Biol. 2004 Jun; 24(11):4734-42. PMID: 15143168; PMCID: PMC416403.
  66. Kim M, Ahn SH, Krogan NJ, Greenblatt JF, Buratowski S. Transitions in RNA polymerase II elongation complexes at the 3' ends of genes. EMBO J. 2004 Jan 28; 23(2):354-64. PMID: 14739930; PMCID: PMC1271760.
  67. Ahn SH, Kim M, Buratowski S. Phosphorylation of serine 2 within the RNA polymerase II C-terminal domain couples transcription and 3' end processing. Mol Cell. 2004 Jan 16; 13(1):67-76. PMID: 14731395.
    View in: PubMed
  68. Keogh MC, Buratowski S. Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in Saccharomyces cerevisiae. Methods Mol Biol. 2004; 257:1-16. PMID: 14769992.
    View in: PubMed
  69. Matangkasombut O, Auty R, Buratowski S. Structure and function of the TFIID complex. Adv Protein Chem. 2004; 67:67-92. PMID: 14969724.
    View in: PubMed
  70. Krogan NJ, Keogh MC, Datta N, Sawa C, Ryan OW, Ding H, Haw RA, Pootoolal J, Tong A, Canadien V, Richards DP, Wu X, Emili A, Hughes TR, Buratowski S, Greenblatt JF. A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1. Mol Cell. 2003 Dec; 12(6):1565-76. PMID: 14690608.
    View in: PubMed
  71. Keogh MC, Podolny V, Buratowski S. Bur1 kinase is required for efficient transcription elongation by RNA polymerase II. Mol Cell Biol. 2003 Oct; 23(19):7005-18. PMID: 12972617; PMCID: PMC193923.
  72. Buratowski S. The CTD code. Nat Struct Biol. 2003 Sep; 10(9):679-80. PMID: 12942140.
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  73. Nedea E, He X, Kim M, Pootoolal J, Zhong G, Canadien V, Hughes T, Buratowski S, Moore CL, Greenblatt J. Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem. 2003 Aug 29; 278(35):33000-10. PMID: 12819204.
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  74. Krogan NJ, Kim M, Tong A, Golshani A, Cagney G, Canadien V, Richards DP, Beattie BK, Emili A, Boone C, Shilatifard A, Buratowski S, Greenblatt J. Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II. Mol Cell Biol. 2003 Jun; 23(12):4207-18. PMID: 12773564; PMCID: PMC427527.
  75. Takagi T, Walker AK, Sawa C, Diehn F, Takase Y, Blackwell TK, Buratowski S. The Caenorhabditis elegans mRNA 5'-capping enzyme. In vitro and in vivo characterization. J Biol Chem. 2003 Apr 18; 278(16):14174-84. PMID: 12576476.
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  76. Matangkasombut O, Buratowski S. Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation. Mol Cell. 2003 Feb; 11(2):353-63. PMID: 12620224.
    View in: PubMed
  77. Buratowski RM, Downs J, Buratowski S. Interdependent interactions between TFIIB, TATA binding protein, and DNA. Mol Cell Biol. 2002 Dec; 22(24):8735-43. PMID: 12446790; PMCID: PMC139873.
  78. Krogan NJ, Kim M, Ahn SH, Zhong G, Kobor MS, Cagney G, Emili A, Shilatifard A, Buratowski S, Greenblatt JF. RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol Cell Biol. 2002 Oct; 22(20):6979-92. PMID: 12242279; PMCID: PMC139818.
  79. Takagi T, Cho EJ, Janoo RT, Polodny V, Takase Y, Keogh MC, Woo SA, Fresco-Cohen LD, Hoffman CS, Buratowski S. Divergent subunit interactions among fungal mRNA 5'-capping machineries. Eukaryot Cell. 2002 Jun; 1(3):448-57. PMID: 12455993; PMCID: PMC118010.
  80. Keogh MC, Cho EJ, Podolny V, Buratowski S. Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation. Mol Cell Biol. 2002 Mar; 22(5):1288-97. PMID: 11839796; PMCID: PMC134711.
  81. Cho EJ, Kobor MS, Kim M, Greenblatt J, Buratowski S. Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain. Genes Dev. 2001 Dec 15; 15(24):3319-29. PMID: 11751637; PMCID: PMC312848.
  82. Selleck W, Howley R, Fang Q, Podolny V, Fried MG, Buratowski S, Tan S. A histone fold TAF octamer within the yeast TFIID transcriptional coactivator. Nat Struct Biol. 2001 Aug; 8(8):695-700. PMID: 11473260.
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  83. Buratowski S, Chodosh LA. Mobility shift DNA-binding assay using gel electrophoresis. Curr Protoc Pharmacol. 2001 Aug; Chapter 6:Unit6.8. PMID: 21959763.
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  84. Buratowski S, Chodosh LA. Mobility shift DNA-binding assay using gel electrophoresis. Curr Protoc Mol Biol. 2001 May; Chapter 12:Unit 12.2. PMID: 18265085.
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  85. Chodosh LA, Buratowski S. Purification of DNA-binding proteins using biotin/streptavidin affinity systems. Curr Protoc Protein Sci. 2001 May; Chapter 9:Unit 9.7. PMID: 18429216.
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  86. Takagi T, Buratowski S. A Plasmodium falciparum protein related to fungal RNA 5'-triphosphatases. Mol Biochem Parasitol. 2001 May; 114(2):239-44. PMID: 11378203.
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  87. Takase Y, Takagi T, Komarnitsky PB, Buratowski S. The essential interaction between yeast mRNA capping enzyme subunits is not required for triphosphatase function in vivo. Mol Cell Biol. 2000 Dec; 20(24):9307-16. PMID: 11094081; PMCID: PMC102187.
  88. Komarnitsky P, Cho EJ, Buratowski S. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription. Genes Dev. 2000 Oct 01; 14(19):2452-60. PMID: 11018013; PMCID: PMC316976.
  89. Buratowski S. Snapshots of RNA polymerase II transcription initiation. Curr Opin Cell Biol. 2000 Jun; 12(3):320-5. PMID: 10801469.
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  90. Matangkasombut O, Buratowski RM, Swilling NW, Buratowski S. Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev. 2000 Apr 15; 14(8):951-62. PMID: 10783167; PMCID: PMC316539.
  91. Rodriguez CR, Cho EJ, Keogh MC, Moore CL, Greenleaf AL, Buratowski S. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol. 2000 Jan; 20(1):104-12. PMID: 10594013; PMCID: PMC85066.
  92. Morehouse H, Buratowski RM, Silver PA, Buratowski S. The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc Natl Acad Sci U S A. 1999 Oct 26; 96(22):12542-7. PMID: 10535958; PMCID: PMC22979.
  93. Komarnitsky PB, Michel B, Buratowski S. TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo. Genes Dev. 1999 Oct 01; 13(19):2484-9. PMID: 10521393; PMCID: PMC317065.
  94. Cho EJ, Buratowski S. Evidence that transcription factor IIB is required for a post-assembly step in transcription initiation. J Biol Chem. 1999 Sep 03; 274(36):25807-13. PMID: 10464320.
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  95. Deshpande T, Takagi T, Hao L, Buratowski S, Charbonneau H. Human PIR1 of the protein-tyrosine phosphatase superfamily has RNA 5'-triphosphatase and diphosphatase activities. J Biol Chem. 1999 Jun 04; 274(23):16590-4. PMID: 10347225.
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  96. Rodriguez CR, Takagi T, Cho EJ, Buratowski S. A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme. Nucleic Acids Res. 1999 May 15; 27(10):2181-8. PMID: 10219091; PMCID: PMC148438.
  97. Cho EJ, Rodriguez CR, Takagi T, Buratowski S. Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain. Genes Dev. 1998 Nov 15; 12(22):3482-7. PMID: 9832501; PMCID: PMC317247.
  98. Michel B, Komarnitsky P, Buratowski S. Histone-like TAFs are essential for transcription in vivo. Mol Cell. 1998 Nov; 2(5):663-73. PMID: 9844638.
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  99. Takagi T, Taylor GS, Kusakabe T, Charbonneau H, Buratowski S. A protein tyrosine phosphatase-like protein from baculovirus has RNA 5'-triphosphatase and diphosphatase activities. Proc Natl Acad Sci U S A. 1998 Aug 18; 95(17):9808-12. PMID: 9707557; PMCID: PMC21418.
  100. Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs. Genes Dev. 1998 Feb 01; 12(3):343-56. PMID: 9450929; PMCID: PMC316480.
  101. Cho EJ, Takagi T, Moore CR, Buratowski S. mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain. Genes Dev. 1997 Dec 15; 11(24):3319-26. PMID: 9407025; PMCID: PMC316800.
  102. Buratowski S. Multiple TATA-binding factors come back into style. Cell. 1997 Oct 03; 91(1):13-5. PMID: 9335330.
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  103. Kuldell NH, Buratowski S. Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions. Mol Cell Biol. 1997 Sep; 17(9):5288-98. PMID: 9271406; PMCID: PMC232379.
  104. Takagi T, Moore CR, Diehn F, Buratowski S. An RNA 5'-triphosphatase related to the protein tyrosine phosphatases. Cell. 1997 Jun 13; 89(6):867-73. PMID: 9200605.
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  105. Sachs AB, Buratowski S. Common themes in translational and transcriptional regulation. Trends Biochem Sci. 1997 Jun; 22(6):189-92. PMID: 9204702.
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  106. Moqtaderi Z, Yale JD, Struhl K, Buratowski S. Yeast homologues of higher eukaryotic TFIID subunits. Proc Natl Acad Sci U S A. 1996 Dec 10; 93(25):14654-8. PMID: 8962109; PMCID: PMC26190.
  107. Fresco LD, Buratowski S. Conditional mutants of the yeast mRNA capping enzyme show that the cap enhances, but is not required for, mRNA splicing. RNA. 1996 Jun; 2(6):584-96. PMID: 8718687; PMCID: PMC1369397.
  108. Buratowski S. Mechanisms of gene activation. Science. 1995 Dec 15; 270(5243):1773-4. PMID: 8525364.
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  109. Wang Z, Buratowski S, Svejstrup JQ, Feaver WJ, Wu X, Kornberg RD, Donahue TF, Friedberg EC. The yeast TFB1 and SSL1 genes, which encode subunits of transcription factor IIH, are required for nucleotide excision repair and RNA polymerase II transcription. Mol Cell Biol. 1995 Apr; 15(4):2288-93. PMID: 7891722; PMCID: PMC230456.
  110. Matsui P, DePaulo J, Buratowski S. An interaction between the Tfb1 and Ssl1 subunits of yeast TFIIH correlates with DNA repair activity. Nucleic Acids Res. 1995 Mar 11; 23(5):767-72. PMID: 7708491; PMCID: PMC306757.
  111. Fresco LD, Buratowski S. Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases. Proc Natl Acad Sci U S A. 1994 Jul 05; 91(14):6624-8. PMID: 8022828; PMCID: PMC44255.
  112. Buratowski S. The basics of basal transcription by RNA polymerase II. Cell. 1994 Apr 08; 77(1):1-3. PMID: 8156586.
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  113. Buratowski S. RNA polymerase III transcription in the yeast Saccharomyces cerevisiae. Genet Eng (N Y). 1994; 16:1-9. PMID: 7765192.
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  114. Feaver WJ, Svejstrup JQ, Bardwell L, Bardwell AJ, Buratowski S, Gulyas KD, Donahue TF, Friedberg EC, Kornberg RD. Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair. Cell. 1993 Dec 31; 75(7):1379-87. PMID: 8269516.
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  115. Buratowski S, Zhou H. Functional domains of transcription factor TFIIB. Proc Natl Acad Sci U S A. 1993 Jun 15; 90(12):5633-7. PMID: 8516312; PMCID: PMC46775.
  116. Buratowski S. DNA repair and transcription: the helicase connection. Science. 1993 Apr 02; 260(5104):37-8. PMID: 8465198.
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  117. Buratowski S, Zhou H. A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB. Cell. 1992 Oct 16; 71(2):221-30. PMID: 1423590.
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  118. Koleske AJ, Buratowski S, Nonet M, Young RA. A novel transcription factor reveals a functional link between the RNA polymerase II CTD and TFIID. Cell. 1992 May 29; 69(5):883-94. PMID: 1591782.
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  119. Buratowski S, Zhou H. Transcription factor IID mutants defective for interaction with transcription factor IIA. Science. 1992 Feb 28; 255(5048):1130-2. PMID: 1546314.
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  120. Buratowski S, Sopta M, Greenblatt J, Sharp PA. RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex. Proc Natl Acad Sci U S A. 1991 Sep 01; 88(17):7509-13. PMID: 1881889; PMCID: PMC52330.
  121. Buratowski S, Sharp PA. Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of the largest subunit. Mol Cell Biol. 1990 Oct; 10(10):5562-4. PMID: 2398901; PMCID: PMC361275.
  122. Hahn S, Buratowski S, Sharp PA, Guarente L. Identification of a yeast protein homologous in function to the mammalian general transcription factor, TFIIA. EMBO J. 1989 Nov; 8(11):3379-82. PMID: 2684641; PMCID: PMC401482.
  123. Hahn S, Buratowski S, Sharp PA, Guarente L. Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions. Cell. 1989 Sep 22; 58(6):1173-81. PMID: 2550146.
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  124. Hahn S, Buratowski S, Sharp PA, Guarente L. Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences. Proc Natl Acad Sci U S A. 1989 Aug; 86(15):5718-22. PMID: 2569738; PMCID: PMC297701.
  125. Buratowski S, Hahn S, Guarente L, Sharp PA. Five intermediate complexes in transcription initiation by RNA polymerase II. Cell. 1989 Feb 24; 56(4):549-61. PMID: 2917366.
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  126. Chodosh LA, Buratowski S, Sharp PA. A yeast protein possesses the DNA-binding properties of the adenovirus major late transcription factor. Mol Cell Biol. 1989 Feb; 9(2):820-2. PMID: 2651905; PMCID: PMC362660.
  127. Buratowski S, Hahn S, Sharp PA, Guarente L. Function of a yeast TATA element-binding protein in a mammalian transcription system. Nature. 1988 Jul 07; 334(6177):37-42. PMID: 3290687.
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