Harvard Catalyst Profiles

Contact, publication, and social network information about Harvard faculty and fellows.

Seth Leo Alper, M.D., Ph.D.

Co-Author

This page shows the publications co-authored by Seth Alper and Carlo Brugnara.
Connection Strength

4.637
  1. Alper SL, Vandorpe DH, Peters LL, Brugnara C. Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes. Blood Cells Mol Dis. 2008 Jul-Aug; 41(1):22-34.
    View in: PubMed
    Score: 0.423
  2. Shmukler BE, Rivera A, Bhargava P, Nishimura K, Hsu A, Kim EH, Trudel M, Rust MB, Hubner CA, Brugnara C, Alper SL. Corrigendum to "Combined genetic disruption of K-Cl cotransporters and Gardos channel KCNN4 rescues erythrocyte dehydration in the SAD mouse model of sickle cell disease" [Blood Cells Mol. Dis. (2019) start page-end page not yet assigned] https://doi.org/10.1016/j.bcmd.2019.102346. Blood Cells Mol Dis. 2020 Mar; 81:102390.
    View in: PubMed
    Score: 0.240
  3. Shmukler BE, Rivera A, Bhargava P, Nishimura K, Kim EH, Hsu A, Wohlgemuth JG, Morton J, Snyder LM, De Franceschi L, Rust MB, Hubner CA, Brugnara C, Alper SL. Genetic disruption of KCC cotransporters in a mouse model of thalassemia intermedia. Blood Cells Mol Dis. 2020 03; 81:102389.
    View in: PubMed
    Score: 0.238
  4. Shmukler BE, Rivera A, Bhargava P, Nishimura K, Hsu A, Kim EH, Trudel M, Rust MB, Hubner CA, Brugnara C, Alper SL. Combined genetic disruption of K-Cl cotransporters and Gardos channel KCNN4 rescues erythrocyte dehydration in the SAD mouse model of sickle cell disease. Blood Cells Mol Dis. 2019 11; 79:102346.
    View in: PubMed
    Score: 0.232
  5. Rivera A, Vandorpe DH, Shmukler BE, Andolfo I, Iolascon A, Archer NM, Shabani E, Auerbach M, Hamerschlak N, Morton J, Wohlgemuth JG, Brugnara C, Snyder LM, Alper SL. Erythrocyte ion content and dehydration modulate maximal Gardos channel activity in KCNN4 V282M/+ hereditary xerocytosis red cells. Am J Physiol Cell Physiol. 2019 08 01; 317(2):C287-C302.
    View in: PubMed
    Score: 0.229
  6. Gnanasambandam R, Rivera A, Vandorpe DH, Shmukler BE, Brugnara C, Snyder LM, Andolfo I, Iolascon A, Silveira PA, Hamerschlak N, Gottlieb P, Alper SL. Increased Red Cell KCNN4 Activity in Sporadic Hereditary Xerocytosis Associated With Enhanced Single Channel Pressure Sensitivity of PIEZO1 Mutant V598M. Hemasphere. 2018 10; 2(5):e55.
    View in: PubMed
    Score: 0.220
  7. Rivera A, Vandorpe DH, Shmukler BE, Gallagher DR, Fikry CC, Kuypers FA, Brugnara C, Snyder LM, Alper SL. Erythrocytes from hereditary xerocytosis patients heterozygous for KCNN4 V282M exhibit increased spontaneous Gardos channel-like activity inhibited by senicapoc. Am J Hematol. 2017 06; 92(6):E108-E110.
    View in: PubMed
    Score: 0.199
  8. Brugnara C, Armsby CC, De Franceschi L, Crest M, Euclaire MF, Alper SL. Ca(2+)-activated K+ channels of human and rabbit erythrocytes display distinctive patterns of inhibition by venom peptide toxins. J Membr Biol. 1995 Sep; 147(1):71-82.
    View in: PubMed
    Score: 0.177
  9. Brugnara C, De Franceschi L, Armsby CC, Saadane N, Trudel M, Beuzard Y, Rittenhouse A, Rifai N, Platt O, Alper SL. A new therapeutic approach for sickle cell disease. Blockade of the red cell Ca(2+)-activated K+ channel by clotrimazole. Ann N Y Acad Sci. 1995 Jul 12; 763:262-71.
    View in: PubMed
    Score: 0.176
  10. Shmukler BE, Vandorpe DH, Rivera A, Auerbach M, Brugnara C, Alper SL. Dehydrated stomatocytic anemia due to the heterozygous mutation R2456H in the mechanosensitive cation channel PIEZO1: a case report. Blood Cells Mol Dis. 2014 Jan; 52(1):53-4.
    View in: PubMed
    Score: 0.154
  11. Brugnara C, de Franceschi L, Alper SL. Inhibition of Ca(2+)-dependent K+ transport and cell dehydration in sickle erythrocytes by clotrimazole and other imidazole derivatives. J Clin Invest. 1993 Jul; 92(1):520-6.
    View in: PubMed
    Score: 0.153
  12. Brugnara C, De Franceschi L, Alper SL. Ca(2+)-activated K+ transport in erythrocytes. Comparison of binding and transport inhibition by scorpion toxins. J Biol Chem. 1993 Apr 25; 268(12):8760-8.
    View in: PubMed
    Score: 0.151
  13. Rivera A, Zee RY, Alper SL, Peters LL, Brugnara C. Strain-specific variations in cation content and transport in mouse erythrocytes. Physiol Genomics. 2013 May 01; 45(9):343-50.
    View in: PubMed
    Score: 0.150
  14. Stewart AK, Shmukler BE, Vandorpe DH, Rivera A, Heneghan JF, Li X, Hsu A, Karpatkin M, O'Neill AF, Bauer DE, Heeney MM, John K, Kuypers FA, Gallagher PG, Lux SE, Brugnara C, Westhoff CM, Alper SL. Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S. Am J Physiol Cell Physiol. 2011 Dec; 301(6):C1325-43.
    View in: PubMed
    Score: 0.134
  15. Stewart AK, Kedar PS, Shmukler BE, Vandorpe DH, Hsu A, Glader B, Rivera A, Brugnara C, Alper SL. Functional characterization and modified rescue of novel AE1 mutation R730C associated with overhydrated cation leak stomatocytosis. Am J Physiol Cell Physiol. 2011 May; 300(5):C1034-46.
    View in: PubMed
    Score: 0.129
  16. Vandorpe DH, Xu C, Shmukler BE, Otterbein LE, Trudel M, Sachs F, Gottlieb PA, Brugnara C, Alper SL. Hypoxia activates a Ca2+-permeable cation conductance sensitive to carbon monoxide and to GsMTx-4 in human and mouse sickle erythrocytes. PLoS One. 2010 Jan 15; 5(1):e8732.
    View in: PubMed
    Score: 0.120
  17. Casula S, Zolotarev AS, Stuart-Tilley AK, Wilhelm S, Shmukler BE, Brugnara C, Alper SL. Chemical crosslinking studies with the mouse Kcc1 K-Cl cotransporter. Blood Cells Mol Dis. 2009 May-Jun; 42(3):233-40.
    View in: PubMed
    Score: 0.114
  18. Casula S, Shmukler BE, Wilhelm S, Stuart-Tilley AK, Su W, Chernova MN, Brugnara C, Alper SL. A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity. J Biol Chem. 2001 Nov 09; 276(45):41870-8.
    View in: PubMed
    Score: 0.067
  19. Brugnara C, De Franceschi L, Bennekou P, Alper SL, Christophersen P. Novel therapies for prevention of erythrocyte dehydration in sickle cell anemia. Drug News Perspect. 2001 May; 14(4):208-20.
    View in: PubMed
    Score: 0.066
  20. Shmukler BE, Brugnara C, Alper SL. Structure and genetic polymorphism of the mouse KCC1 gene. Biochim Biophys Acta. 2000 Jul 24; 1492(2-3):353-61.
    View in: PubMed
    Score: 0.062
  21. Su W, Shmukler BE, Chernova MN, Stuart-Tilley AK, de Franceschi L, Brugnara C, Alper SL. Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation. Am J Physiol. 1999 11; 277(5):C899-912.
    View in: PubMed
    Score: 0.059
  22. Tanphaichitr VS, Sumboonnanonda A, Ideguchi H, Shayakul C, Brugnara C, Takao M, Veerakul G, Alper SL. Novel AE1 mutations in recessive distal renal tubular acidosis. Loss-of-function is rescued by glycophorin A. J Clin Invest. 1998 Dec 15; 102(12):2173-9.
    View in: PubMed
    Score: 0.056
  23. Vandorpe DH, Shmukler BE, Jiang L, Lim B, Maylie J, Adelman JP, de Franceschi L, Cappellini MD, Brugnara C, Alper SL. cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation. J Biol Chem. 1998 Aug 21; 273(34):21542-53.
    View in: PubMed
    Score: 0.055
  24. Jarolim P, Shayakul C, Prabakaran D, Jiang L, Stuart-Tilley A, Rubin HL, Simova S, Zavadil J, Herrin JT, Brouillette J, Somers MJ, Seemanova E, Brugnara C, Guay-Woodford LM, Alper SL. Autosomal dominant distal renal tubular acidosis is associated in three families with heterozygosity for the R589H mutation in the AE1 (band 3) Cl-/HCO3- exchanger. J Biol Chem. 1998 Mar 13; 273(11):6380-8.
    View in: PubMed
    Score: 0.053
  25. Kaufman HW, Niles JK, Gallagher DR, Rivera A, Alper SL, Brugnara C, Snyder LM. Revised prevalence estimate of possible Hereditary Xerocytosis as derived from a large U.S. Laboratory database. Am J Hematol. 2018 01; 93(1):E9-E12.
    View in: PubMed
    Score: 0.052
  26. De Franceschi L, Bachir D, Galacteros F, Tchernia G, Cynober T, Alper S, Platt O, Beuzard Y, Brugnara C. Oral magnesium supplements reduce erythrocyte dehydration in patients with sickle cell disease. J Clin Invest. 1997 Oct 01; 100(7):1847-52.
    View in: PubMed
    Score: 0.051
  27. Rittenhouse AR, Parker C, Brugnara C, Morgan KG, Alper SL. Inhibition of maxi-K currents in ferret portal vein smooth muscle cells by the antifungal clotrimazole. Am J Physiol. 1997 Jul; 273(1 Pt 1):C45-56.
    View in: PubMed
    Score: 0.050
  28. Rittenhouse AR, Vandorpe DH, Brugnara C, Alper SL. The antifungal imidazole clotrimazole and its major in vivo metabolite are potent blockers of the calcium-activated potassium channel in murine erythroleukemia cells. J Membr Biol. 1997 May 15; 157(2):177-91.
    View in: PubMed
    Score: 0.050
  29. Ilboudo Y, Bartolucci P, Rivera A, Sedzro JC, Beaudoin M, Trudel M, Alper SL, Brugnara C, Galactéros F, Lettre G. Genome-wide association study of erythrocyte density in sickle cell disease patients. Blood Cells Mol Dis. 2017 06; 65:60-65.
    View in: PubMed
    Score: 0.050
  30. Shmukler B, Sun T, Brugnara C, Alper SL. Reinterpretation of the RACTK1 K+ channel. Am J Physiol. 1997 Jan; 272(1 Pt 1):C350-4.
    View in: PubMed
    Score: 0.049
  31. Andolfo I, Russo R, Manna F, De Rosa G, Gambale A, Zouwail S, Detta N, Pardo CL, Alper SL, Brugnara C, Sharma AK, De Franceschi L, Iolascon A. Functional characterization of novel ABCB6 mutations and their clinical implications in familial pseudohyperkalemia. Haematologica. 2016 08; 101(8):909-17.
    View in: PubMed
    Score: 0.047
  32. Brugnara C, Gee B, Armsby CC, Kurth S, Sakamoto M, Rifai N, Alper SL, Platt OS. Therapy with oral clotrimazole induces inhibition of the Gardos channel and reduction of erythrocyte dehydration in patients with sickle cell disease. J Clin Invest. 1996 Mar 01; 97(5):1227-34.
    View in: PubMed
    Score: 0.046
  33. Armsby CC, Stuart-Tilley AK, Alper SL, Brugnara C. Resistance to osmotic lysis in BXD-31 mouse erythrocytes: association with upregulated K-Cl cotransport. Am J Physiol. 1996 Mar; 270(3 Pt 1):C866-77.
    View in: PubMed
    Score: 0.046
  34. Tubman VN, Mejia P, Shmukler BE, Bei AK, Alper SL, Mitchell JR, Brugnara C, Duraisingh MT. The Clinically Tested Gardos Channel Inhibitor Senicapoc Exhibits Antimalarial Activity. Antimicrob Agents Chemother. 2016 01; 60(1):613-6.
    View in: PubMed
    Score: 0.045
  35. Andolfo I, Russo R, Manna F, Shmukler BE, Gambale A, Vitiello G, De Rosa G, Brugnara C, Alper SL, Snyder LM, Iolascon A. Novel Gardos channel mutations linked to dehydrated hereditary stomatocytosis (xerocytosis). Am J Hematol. 2015 Oct; 90(10):921-6.
    View in: PubMed
    Score: 0.045
  36. Coupry I, Armsby CC, Alper S, Parini A, Brugnara C. Inhibition of red cell Ca(2+)-activated K+ transport by clotrimazole does not take place via imidazoline binding sites. Ann N Y Acad Sci. 1995 Jul 12; 763:287-9.
    View in: PubMed
    Score: 0.044
  37. Brugnara C, Armsby CC, Sakamoto M, Rifai N, Alper SL, Platt O. Oral administration of clotrimazole and blockade of human erythrocyte Ca(++)-activated K+ channel: the imidazole ring is not required for inhibitory activity. J Pharmacol Exp Ther. 1995 Apr; 273(1):266-72.
    View in: PubMed
    Score: 0.043
  38. Armsby CC, Brugnara C, Alper SL. Cation transport in mouse erythrocytes: role of K(+)-Cl- cotransport in regulatory volume decrease. Am J Physiol. 1995 Apr; 268(4 Pt 1):C894-902.
    View in: PubMed
    Score: 0.043
  39. Archer NM, Shmukler BE, Andolfo I, Vandorpe DH, Gnanasambandam R, Higgins JM, Rivera A, Fleming MD, Sachs F, Gottlieb PA, Iolascon A, Brugnara C, Alper SL, Nathan DG. Hereditary xerocytosis revisited. Am J Hematol. 2014 Dec; 89(12):1142-6.
    View in: PubMed
    Score: 0.041
  40. Jarolim P, Rubin HL, Liu SC, Cho MR, Brabec V, Derick LH, Yi SJ, Saad ST, Alper S, Brugnara C, et al. Duplication of 10 nucleotides in the erythroid band 3 (AE1) gene in a kindred with hereditary spherocytosis and band 3 protein deficiency (band 3PRAGUE). J Clin Invest. 1994 Jan; 93(1):121-30.
    View in: PubMed
    Score: 0.040
  41. Andolfo I, Alper SL, De Franceschi L, Auriemma C, Russo R, De Falco L, Vallefuoco F, Esposito MR, Vandorpe DH, Shmukler BE, Narayan R, Montanaro D, D'Armiento M, Vetro A, Limongelli I, Zuffardi O, Glader BE, Schrier SL, Brugnara C, Stewart GW, Delaunay J, Iolascon A. Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1. Blood. 2013 May 09; 121(19):3925-35, S1-12.
    View in: PubMed
    Score: 0.037
  42. Andolfo I, Alper SL, Delaunay J, Auriemma C, Russo R, Asci R, Esposito MR, Sharma AK, Shmukler BE, Brugnara C, De Franceschi L, Iolascon A. Missense mutations in the ABCB6 transporter cause dominant familial pseudohyperkalemia. Am J Hematol. 2013 Jan; 88(1):66-72.
    View in: PubMed
    Score: 0.037
  43. Rust MB, Alper SL, Rudhard Y, Shmukler BE, Vicente R, Brugnara C, Trudel M, Jentsch TJ, Hübner CA. Disruption of erythroid K-Cl cotransporters alters erythrocyte volume and partially rescues erythrocyte dehydration in SAD mice. J Clin Invest. 2007 Jun; 117(6):1708-17.
    View in: PubMed
    Score: 0.025
  44. De Franceschi L, Villa-Moruzzi E, Biondani A, Siciliano A, Brugnara C, Alper SL, Lowell CA, Berton G. Regulation of K-Cl cotransport by protein phosphatase 1alpha in mouse erythrocytes. Pflugers Arch. 2006 Mar; 451(6):760-8.
    View in: PubMed
    Score: 0.022
  45. Begenisich T, Nakamoto T, Ovitt CE, Nehrke K, Brugnara C, Alper SL, Melvin JE. Physiological roles of the intermediate conductance, Ca2+-activated potassium channel Kcnn4. J Biol Chem. 2004 Nov 12; 279(46):47681-7.
    View in: PubMed
    Score: 0.021
  46. De Franceschi L, Villa-Moruzzi E, Fumagalli L, Brugnara C, Turrini F, Motta R, Veghini E, Corato C, Alper SL, Berton G. K-Cl cotransport modulation by intracellular Mg in erythrocytes from mice bred for low and high Mg levels. Am J Physiol Cell Physiol. 2001 Oct; 281(4):C1385-95.
    View in: PubMed
    Score: 0.017
  47. Rufo PA, Merlin D, Riegler M, Ferguson-Maltzman MH, Dickinson BL, Brugnara C, Alper SL, Lencer WI. The antifungal antibiotic, clotrimazole, inhibits chloride secretion by human intestinal T84 cells via blockade of distinct basolateral K+ conductances. Demonstration of efficacy in intact rabbit colon and in an in vivo mouse model of cholera. J Clin Invest. 1997 Dec 15; 100(12):3111-20.
    View in: PubMed
    Score: 0.013
  48. Rufo PA, Jiang L, Moe SJ, Brugnara C, Alper SL, Lencer WI. The antifungal antibiotic, clotrimazole, inhibits Cl- secretion by polarized monolayers of human colonic epithelial cells. J Clin Invest. 1996 Nov 01; 98(9):2066-75.
    View in: PubMed
    Score: 0.012
  49. Peters LL, Shivdasani RA, Liu SC, Hanspal M, John KM, Gonzalez JM, Brugnara C, Gwynn B, Mohandas N, Alper SL, Orkin SH, Lux SE. Anion exchanger 1 (band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton. Cell. 1996 Sep 20; 86(6):917-27.
    View in: PubMed
    Score: 0.012
  50. de Franceschi L, Rouyer-Fessard P, Alper SL, Jouault H, Brugnara C, Beuzard Y. Combination therapy of erythropoietin, hydroxyurea, and clotrimazole in a beta thalassemic mouse: a model for human therapy. Blood. 1996 Feb 01; 87(3):1188-95.
    View in: PubMed
    Score: 0.011
  51. Coupry I, Armsby CC, Alper SL, Brugnara C, Parini A. Clotrimazole and efaroxan inhibit red cell Gardos channel independently of imidazoline I1 and I2 binding sites. Eur J Pharmacol. 1996 Jan 04; 295(1):109-12.
    View in: PubMed
    Score: 0.011
  52. Jarolim P, Rubin HL, Brabec V, Chrobak L, Zolotarev AS, Alper SL, Brugnara C, Wichterle H, Palek J. Mutations of conserved arginines in the membrane domain of erythroid band 3 lead to a decrease in membrane-associated band 3 and to the phenotype of hereditary spherocytosis. Blood. 1995 Feb 01; 85(3):634-40.
    View in: PubMed
    Score: 0.011
  53. De Franceschi L, Saadane N, Trudel M, Alper SL, Brugnara C, Beuzard Y. Treatment with oral clotrimazole blocks Ca(2+)-activated K+ transport and reverses erythrocyte dehydration in transgenic SAD mice. A model for therapy of sickle cell disease. J Clin Invest. 1994 Apr; 93(4):1670-6.
    View in: PubMed
    Score: 0.010
Connection Strength
The connection strength for co-authors is the sum of the scores for each of their shared publications.

Publication scores are based on many factors, including how long ago they were written and whether the person is a first or senior author.
Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.