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Susan James Hagen, Ph.D.


Available: 01/10/22, Expires: 03/01/24

Them1, or acyl-CoA thioesterase, regulates metabolism by forming puncta in cells that change to a diffuse pattern after stimulation by cold exposure or after eating a high fat diet. The aim of this project would be to characterize the nature of puncta by using biophysics and cell/molecular biology methods. Biomolecular condensates form by liquid-liquid phase transition in cells by increasing concentration, temperature, pH, or by other properties of the protein. The student would thus use use purified Them1 and microscopy techniques to determine how Them1 phase separates into biomolecular condensates and then test the learned principles in cultured cells. The student would do hands-on bench experiments and learn to use the confocal microsocope. They would design experiments after reading papers, present the design to the PI and senior lab manager, carry-out experiments with help/supervision if needed, analyze data, present data at a weekly lab meeting, (if successful) present data at a National/International scientific meeting and write this section/co-author a paper for publication. Some wet bench skills in advance including pipetting and designing experiments would be helpful.

Role of Ammonia and H. Pylori Vacuolating Cytotoxin in Restitution
Full Time/Year Long, 07/01/98 - 06/30/99

The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R01DK103046 (COHEN, DAVID E.) Jul 5, 2015 - Jun 30, 2024
    Them1-Mediated Metabolic Regulation and Pathogenic Role in NAFLD
    Role: Multi-PI
  2. S10OD019988 (HAGEN, SUSAN J) Apr 1, 2015 - Mar 31, 2016
    Wohlwend High Pressure Freezer for the BIDMC Electron Microscopy Core
    Role: Principal Investigator
  3. S10RR017927 (HAGEN, SUSAN J) May 1, 2006 - Apr 30, 2007
    Confocal Microscope for the BIDMC Imaging Core Facility
    Role: Principal Investigator
  4. R55DK044287 (HAGEN, SUSAN J) Sep 30, 1991 - Sep 29, 1993
    Role: Principal Investigator
  5. P30DK034854 (LENCER, WAYNE I) Sep 30, 1984 - Mar 31, 2026
    Integrated Epithelial and Muscosal Biology
    Role: Co-Principal Investigator

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Kalashyan M, Raghunathan K, Oller H, Theres MB, Jimenez L, Roland JT, Kolobova E, Hagen SJ, Goldsmith JD, Shub MD, Goldenring JR, Kaji I, Thiagarajah JR. Therapy Development for Microvillus Inclusion Disease using Patient-derived Enteroids. bioRxiv. 2023 Jan 29. PMID: 36747680; PMCID: PMC9900906.
  2. Hagen SJ. Gastroduodenal injury and repair: novel targets for therapeutic intervention. Curr Opin Gastroenterol. 2022 11 01; 38(6):607-612. PMID: 36219128; PMCID: PMC9558095.
    Citations:    Fields:    Translation:HumansAnimalsCells
  3. Hagen SJ. Mucosal defense: gastroduodenal injury and repair mechanisms. Curr Opin Gastroenterol. 2021 11 01; 37(6):609-614. PMID: 34475337; PMCID: PMC8511296.
    Citations: 2     Fields:    Translation:Humans
  4. Li Y, Imai N, Nicholls HT, Roberts BR, Goyal S, Krisko TI, Ang LH, Tillman MC, Roberts AM, Baqai M, Ortlund EA, Cohen DE, Hagen SJ. Thioesterase superfamily member 1 undergoes stimulus-coupled conformational reorganization to regulate metabolism in mice. Nat Commun. 2021 06 09; 12(1):3493. PMID: 34108467; PMCID: PMC8190112.
    Citations:    Fields:    Translation:AnimalsCells
  5. Raya-Sandino A, Luissint AC, Kusters DHM, Narayanan V, Flemming S, Garcia-Hernandez V, Godsel LM, Green KJ, Hagen SJ, Conway DE, Parkos CA, Nusrat A. Regulation of intestinal epithelial intercellular adhesion and barrier function by desmosomal cadherin desmocollin-2. Mol Biol Cell. 2021 04 15; 32(8):753-768. PMID: 33596089; PMCID: PMC8108520.
    Citations: 9     Fields:    Translation:HumansAnimalsCells
  6. Caron TJ, Scott KE, Sinha N, Muthupalani S, Baqai M, Ang LH, Li Y, Turner JR, Fox JG, Hagen SJ. Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells. Cell Mol Gastroenterol Hepatol. 2021; 11(3):783-801. PMID: 33069918.
    Citations: 3     Fields:    Translation:AnimalsCells
  7. Wang CH, Lundh M, Fu A, Kriszt R, Huang TL, Lynes MD, Leiria LO, Shamsi F, Darcy J, Greenwood BP, Narain NR, Tolstikov V, Smith KL, Emanuelli B, Chang YT, Hagen S, Danial NN, Kiebish MA, Tseng YH. CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice. Sci Transl Med. 2020 08 26; 12(558). PMID: 32848096.
    Citations: 29     Fields:    Translation:HumansAnimalsCells
  8. Tillman MC, Imai N, Li Y, Khadka M, Okafor CD, Juneja P, Adhiyaman A, Hagen SJ, Cohen DE, Ortlund EA. Allosteric regulation of thioesterase superfamily member 1 by lipid sensor domain binding fatty acids and lysophosphatidylcholine. Proc Natl Acad Sci U S A. 2020 09 08; 117(36):22080-22089. PMID: 32820071.
    Citations: 3     Fields:    Translation:HumansCells
  9. Hagen SJ. Do Ulcers Heal Structurally and Functionally? Dig Dis Sci. 2020 01; 65(1):3-5. PMID: 31707528.
    Citations:    Fields:    Translation:Humans
  10. Muthupalani S, Ge Z, Joy J, Feng Y, Dobey C, Cho HY, Langenbach R, Wang TC, Hagen SJ, Fox JG. Muc5ac null mice are predisposed to spontaneous gastric antro-pyloric hyperplasia and adenomas coupled with attenuated H. pylori-induced corpus mucous metaplasia. Lab Invest. 2019 12; 99(12):1887-1905. PMID: 31399638; PMCID: PMC6927550.
    Citations: 6     Fields:    Translation:AnimalsCells
  11. Hagen SJ. Unraveling a New Role for Claudins in Gastric Tumorigenesis. Cell Mol Gastroenterol Hepatol. 2019; 8(1):151-152. PMID: 31047875; PMCID: PMC6599093.
    Citations: 1     Fields:    Translation:Animals
  12. Hagen SJ, Ang LH, Zheng Y, Karahan SN, Wu J, Wang YE, Caron TJ, Gad AP, Muthupalani S, Fox JG. Loss of Tight Junction Protein Claudin 18 Promotes Progressive Neoplasia Development in Mouse Stomach. Gastroenterology. 2018 12; 155(6):1852-1867. PMID: 30195448; PMCID: PMC6613545.
    Citations: 35     Fields:    Translation:AnimalsCells
  13. Desai A, Alves-Bezerra M, Li Y, Ozdemir C, Bare CJ, Li Y, Hagen SJ, Cohen DE. Regulation of fatty acid trafficking in liver by thioesterase superfamily member 1. J Lipid Res. 2018 02; 59(2):368-379. PMID: 29208699.
    Citations: 2     Fields:    Translation:Animals
  14. Palmer CJ, Bruckner RJ, Paulo JA, Kazak L, Long JZ, Mina AI, Deng Z, LeClair KB, Hall JA, Hong S, Zushin PH, Smith KL, Gygi SP, Hagen S, Cohen DE, Banks AS. Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue. Mol Metab. 2017 10; 6(10):1212-1225. PMID: 29031721; PMCID: PMC5641635.
    Citations: 25     Fields:    Translation:AnimalsCells
  15. Hagen SJ. Non-canonical functions of claudin proteins: Beyond the regulation of cell-cell adhesions. Tissue Barriers. 2017 04 03; 5(2):e1327839. PMID: 28548895.
    Citations: 37     Fields:    Translation:Humans
  16. Okada K, LeClair KB, Zhang Y, Li Y, Ozdemir C, Krisko TI, Hagen SJ, Betensky RA, Banks AS, Cohen DE. Thioesterase superfamily member 1 suppresses cold thermogenesis by limiting the oxidation of lipid droplet-derived fatty acids in brown adipose tissue. Mol Metab. 2016 May; 5(5):340-351. PMID: 27110486.
    Citations: 22     Fields:    
  17. Caron TJ, Scott KE, Fox JG, Hagen SJ. Tight junction disruption: Helicobacter pylori and dysregulation of the gastric mucosal barrier. World J Gastroenterol. 2015 Oct 28; 21(40):11411-27. PMID: 26523106; PMCID: PMC4616217.
    Citations: 26     Fields:    Translation:HumansAnimalsCells
  18. Adolph TE, Tomczak MF, Niederreiter L, Ko HJ, Böck J, Martinez-Naves E, Glickman JN, Tschurtschenthaler M, Hartwig J, Hosomi S, Flak MB, Cusick JL, Kohno K, Iwawaki T, Billmann-Born S, Raine T, Bharti R, Lucius R, Kweon MN, Marciniak SJ, Choi A, Hagen SJ, Schreiber S, Rosenstiel P, Kaser A, Blumberg RS. Paneth cells as a site of origin for intestinal inflammation. Nature. 2013 Nov 14; 503(7475):272-6. PMID: 24089213; PMCID: PMC3862182.
    Citations: 335     Fields:    Translation:AnimalsCells
  19. Kang HW, Ozdemir C, Kawano Y, LeClair KB, Vernochet C, Kahn CR, Hagen SJ, Cohen DE. Thioesterase superfamily member 2/Acyl-CoA thioesterase 13 (Them2/Acot13) regulates adaptive thermogenesis in mice. J Biol Chem. 2013 Nov 15; 288(46):33376-86. PMID: 24072708.
    Citations: 19     Fields:    Translation:AnimalsCells
  20. Seo JH, Fox JG, Peek RM, Hagen SJ. N-methyl D-aspartate channels link ammonia and epithelial cell death mechanisms in Helicobacter pylori Infection. Gastroenterology. 2011 Dec; 141(6):2064-75. PMID: 21925124; PMCID: PMC3658443.
    Citations: 8     Fields:    Translation:AnimalsCells
  21. Song P, Groos S, Riederer B, Feng Z, Krabbenhöft A, Manns MP, Smolka A, Hagen SJ, Neusch C, Seidler U. Kir4.1 channel expression is essential for parietal cell control of acid secretion. J Biol Chem. 2011 Apr 22; 286(16):14120-8. PMID: 21367857.
    Citations: 12     Fields:    Translation:AnimalsCells
  22. Shanahan MT, Vidrich A, Shirafuji Y, Dubois CL, Henschen-Edman A, Hagen SJ, Cohn SM, Ouellette AJ. Elevated expression of Paneth cell CRS4C in ileitis-prone SAMP1/YitFc mice: regional distribution, subcellular localization, and mechanism of action. J Biol Chem. 2010 Mar 05; 285(10):7493-504. PMID: 20056603.
    Citations: 9     Fields:    Translation:AnimalsCells
  23. Kang HW, Ribich S, Kim BW, Hagen SJ, Bianco AC, Cohen DE. Mice lacking Pctp /StarD2 exhibit increased adaptive thermogenesis and enlarged mitochondria in brown adipose tissue. J Lipid Res. 2009 Nov; 50(11):2212-21. PMID: 19502644.
    Citations: 17     Fields:    Translation:AnimalsCells
  24. Hagen SJ, Ohtani M, Zhou JR, Taylor NS, Rickman BH, Blackburn GL, Fox JG. Inflammation and foveolar hyperplasia are reduced by supplemental dietary glutamine during Helicobacter pylori infection in mice. J Nutr. 2009 May; 139(5):912-8. PMID: 19261732; PMCID: PMC2714391.
    Citations: 9     Fields:    Translation:AnimalsCells
  25. Hayashi S, Nakamura E, Kubo Y, Takahashi N, Yamaguchi A, Matsui H, Hagen SJ, Takeuchi K. Impairment by allyl isothiocyanate of gastric epithelial wound repair through inhibition of ion transporters. J Physiol Pharmacol. 2008 Dec; 59(4):691-706. PMID: 19212004.
    Citations: 6     Fields:    Translation:AnimalsCells
  26. Tashima K, Zhang S, Ragasa R, Nakamura E, Seo JH, Muvaffak A, Hagen SJ. Hepatocyte growth factor regulates the development of highly pure cultured chief cells from rat stomach by stimulating chief cell proliferation in vitro. Am J Physiol Gastrointest Liver Physiol. 2009 Feb; 296(2):G319-29. PMID: 19023030.
    Citations: 3     Fields:    Translation:HumansAnimalsCells
  27. Hagen SJ, Yang DX, Tashima K, Taylor NS, Fox JG. Epithelial cell expression of BCL-2 family proteins predicts mechanisms that regulate Helicobacter pylori-induced pathology in the mouse stomach. Lab Invest. 2008 Nov; 88(11):1227-44. PMID: 18779780; PMCID: PMC2766781.
    Citations: 3     Fields:    Translation:AnimalsCells
  28. Kaufhold MA, Krabbenhöft A, Song P, Engelhardt R, Riederer B, Fährmann M, Klöcker N, Beil W, Manns M, Hagen SJ, Seidler U. Localization, trafficking, and significance for acid secretion of parietal cell Kir4.1 and KCNQ1 K+ channels. Gastroenterology. 2008 Apr; 134(4):1058-69. PMID: 18395087.
    Citations: 19     Fields:    Translation:AnimalsCells
  29. Ragasa R, Nakamura E, Marrone L, Yanaka S, Hayashi S, Takeuchi K, Hagen SJ. Isothiocyanate inhibits restitution and wound repair after injury in the stomach: ex vivo and in vitro studies. J Pharmacol Exp Ther. 2007 Oct; 323(1):1-9. PMID: 17609422.
    Citations: 3     Fields:    Translation:AnimalsCells
  30. Hagen SJ, Morrison SW, Law CS, Yang DX. Restitution of the bullfrog gastric mucosa is dependent on a DIDS-inhibitable pathway not related to HCO3- ion transport. Am J Physiol Gastrointest Liver Physiol. 2004 Apr; 286(4):G596-605. PMID: 14604862.
    Citations: 6     Fields:    Translation:AnimalsCells
  31. Nakamura E, Hagen SJ. Role of glutamine and arginase in protection against ammonia-induced cell death in gastric epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2002 Dec; 283(6):G1264-75. PMID: 12388179.
    Citations: 14     Fields:    Translation:AnimalsCells
  32. He D, Sougioultzis S, Hagen S, Liu J, Keates S, Keates AC, Pothoulakis C, Lamont JT. Clostridium difficile toxin A triggers human colonocyte IL-8 release via mitochondrial oxygen radical generation. Gastroenterology. 2002 Apr; 122(4):1048-57. PMID: 11910356.
    Citations: 44     Fields:    Translation:HumansCells
  33. Abdul-Ghaffar Al-Shaibani TA, Hagen SJ. Regulation of acid secretion and paracellular permeability by F-actin in the bullfrog, Rana catesbeiana. Am J Physiol Gastrointest Liver Physiol. 2002 Mar; 282(3):G519-26. PMID: 11842002.
    Citations: 1     Fields:    Translation:AnimalsCells
  34. Ayabe T, Satchell DP, Pesendorfer P, Tanabe H, Wilson CL, Hagen SJ, Ouellette AJ. Activation of Paneth cell alpha-defensins in mouse small intestine. J Biol Chem. 2002 Feb 15; 277(7):5219-28. PMID: 11733520.
    Citations: 78     Fields:    Translation:AnimalsCells
  35. Cheng AM, Morrison SW, Yang DX, Hagen SJ. Energy dependence of restitution in the gastric mucosa. Am J Physiol Cell Physiol. 2001 Aug; 281(2):C430-8. PMID: 11443042.
    Citations: 10     Fields:    Translation:AnimalsCells
  36. Ouellette AJ, Satchell DP, Hsieh MM, Hagen SJ, Selsted ME. Characterization of luminal paneth cell alpha-defensins in mouse small intestine. Attenuated antimicrobial activities of peptides with truncated amino termini. J Biol Chem. 2000 Oct 27; 275(43):33969-73. PMID: 10942762.
    Citations: 26     Fields:    Translation:AnimalsCells
  37. Hagen SJ, Wu H, Morrison SW. NH(4)Cl inhibition of acid secretion: possible involvement of an apical K(+) channel in bullfrog oxyntic cells. Am J Physiol Gastrointest Liver Physiol. 2000 Aug; 279(2):G400-10. PMID: 10915650.
    Citations: 3     Fields:    Translation:AnimalsCells
  38. He D, Hagen SJ, Pothoulakis C, Chen M, Medina ND, Warny M, LaMont JT. Clostridium difficile toxin A causes early damage to mitochondria in cultured cells. Gastroenterology. 2000 Jul; 119(1):139-50. PMID: 10889163.
    Citations: 35     Fields:    Translation:AnimalsCells
  39. Saubermann LJ, Beck P, De Jong YP, Pitman RS, Ryan MS, Kim HS, Exley M, Snapper S, Balk SP, Hagen SJ, Kanauchi O, Motoki K, Sakai T, Terhorst C, Koezuka Y, Podolsky DK, Blumberg RS. Activation of natural killer T cells by alpha-galactosylceramide in the presence of CD1d provides protection against colitis in mice. Gastroenterology. 2000 Jul; 119(1):119-28. PMID: 10889161.
    Citations: 64     Fields:    Translation:AnimalsCells
  40. Esteves MI, Schrenzel MD, Marini RP, Taylor NS, Xu S, Hagen S, Feng Y, Shen Z, Fox JG. Helicobacter pylori gastritis in cats with long-term natural infection as a model of human disease. Am J Pathol. 2000 Feb; 156(2):709-21. PMID: 10666399; PMCID: PMC1850051.
    Citations: 4     Fields:    Translation:AnimalsCells
  41. Unno N, Menconi MJ, Smith M, Hagen SJ, Brown DA, Aguirre DE, Fink MP. Acidic conditions ameliorate both adenosine triphosphate depletion and the development of hyperpermeability in cultured Caco-2BBe enterocytic monolayers subjected to metabolic inhibition. Surgery. 1997 Jun; 121(6):668-80. PMID: 9186468.
    Citations: 2     Fields:    Translation:HumansCells
  42. Hagen SJ, Takahashi S, Jansons R. Role of vacuolation in the death of gastric epithelial cells. Am J Physiol. 1997 Jan; 272(1 Pt 1):C48-58. PMID: 9038810.
    Citations: 9     Fields:    Translation:AnimalsCells
  43. Unno N, Menconi MJ, Salzman AL, Smith M, Hagen S, Ge Y, Ezzell RM, Fink MP. Hyperpermeability and ATP depletion induced by chronic hypoxia or glycolytic inhibition in Caco-2BBe monolayers. Am J Physiol. 1996 Jun; 270(6 Pt 1):G1010-21. PMID: 8764209.
    Citations: 15     Fields:    Translation:HumansCells
  44. Crawford JM, Möckel GM, Crawford AR, Hagen SJ, Hatch VC, Barnes S, Godleski JJ, Carey MC. Imaging biliary lipid secretion in the rat: ultrastructural evidence for vesiculation of the hepatocyte canalicular membrane. J Lipid Res. 1995 Oct; 36(10):2147-63. PMID: 8576641.
    Citations: 24     Fields:    Translation:AnimalsCells
  45. Hagen SJ, Yanaka A, Jansons R. Localization of brush border cytoskeletal proteins in gastric oxynticopeptic cells from the bullfrog Rana catesbeiana. Cell Tissue Res. 1994 Feb; 275(2):255-67. PMID: 8111837.
    Citations:    Fields:    Translation:AnimalsCells
  46. Hagen SJ, Trier JS, Dambrauskas R. Exposure of the rat small intestine to raw kidney beans results in reorganization of absorptive cell microvilli. Gastroenterology. 1994 Jan; 106(1):73-84. PMID: 8276211.
    Citations: 6     Fields:    Translation:AnimalsCells
  47. Ouellette AJ, Frederick D, Hagen SJ, Katz JD. Class II antigen-associated invariant chain mRNA in mouse small intestine. Biochem Biophys Res Commun. 1991 Sep 30; 179(3):1642-8. PMID: 1718272.
    Citations:    Fields:    Translation:AnimalsCells
  48. Bleicher PA, Balk SP, Hagen SJ, Blumberg RS, Flotte TJ, Terhorst C. Expression of murine CD1 on gastrointestinal epithelium. Science. 1990 Nov 02; 250(4981):679-82. PMID: 1700477.
    Citations: 54     Fields:    Translation:AnimalsCells
  49. Hagen SJ. Lowicryl embedding of gastrointestinal tissues. J Electron Microsc Tech. 1990 Sep; 16(1):37-44. PMID: 2213230.
    Citations: 1     Fields:    Translation:Animals
  50. Trier JS, Allan CH, Abrahamson DR, Hagen SJ. Epithelial basement membrane of mouse jejunum. Evidence for laminin turnover along the entire crypt-villus axis. J Clin Invest. 1990 Jul; 86(1):87-95. PMID: 2195064; PMCID: PMC296694.
    Citations: 17     Fields:    Translation:Animals
  51. Weinman MD, Allan CH, Trier JS, Hagen SJ. Repair of microvilli in the rat small intestine after damage with lectins contained in the red kidney bean. Gastroenterology. 1989 Nov; 97(5):1193-204. PMID: 2792657.
    Citations: 13     Fields:    Translation:AnimalsCells
  52. Hagen SJ, Trier JS. Immunocytochemical localization of actin in epithelial cells of rat small intestine by light and electron microscopy. J Histochem Cytochem. 1988 Jul; 36(7):717-27. PMID: 3290330.
    Citations: 11     Fields:    Translation:AnimalsCells
  53. Hagen SJ, Allan CH, Trier JS. Demonstration of microtubules in the terminal web of mature absorptive cells from the small intestine of the rat. Cell Tissue Res. 1987 Jun; 248(3):709-11. PMID: 3607856.
    Citations: 6     Fields:    Translation:AnimalsCells
  54. Bjorkman DJ, Allan CH, Hagen SJ, Trier JS. Structural features of absorptive cell and microvillus membrane preparations from rat small intestine. Gastroenterology. 1986 Dec; 91(6):1401-14. PMID: 3533700.
    Citations: 10     Fields:    Translation:AnimalsCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.