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Stefano Gianni, M.D.

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Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Laursen L, Kliche J, Gianni S, Jemth P. Supertertiary protein structure affects an allosteric network. Proc Natl Acad Sci U S A. 2020 Sep 29; 117(39):24294-24304. PMID: 32929026.
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  2. Toto A, Ma S, Malagrinò F, Visconti L, Pagano L, Stromgaard K, Gianni S. Comparing the binding properties of peptides mimicking the Envelope protein of SARS-CoV and SARS-CoV-2 to the PDZ domain of the tight junction-associated PALS1 protein. Protein Sci. 2020 10; 29(10):2038-2042. PMID: 32822073.
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  3. Safaee Fakhr B, Wiegand SB, Pinciroli R, Gianni S, Morais CCA, Ikeda T, Miyazaki Y, Marutani E, Di Fenza R, Larson GM, Parcha V, Gibson LE, Chang MG, Arora P, Carroll RW, Kacmarek RM, Ichinose F, Barth WH, Kaimal A, Hohmann EL, Zapol WM, Berra L. High Concentrations of Nitric Oxide Inhalation Therapy in Pregnant Patients With Severe Coronavirus Disease 2019 (COVID-19). Obstet Gynecol. 2020 Aug 26. PMID: 32852324.
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  4. Gianni S, Morais CCA, Larson G, Pinciroli R, Carroll R, Yu B, Zapol WM, Berra L. Ideation and assessment of a nitric oxide delivery system for spontaneously breathing subjects. Nitric Oxide. 2020 Aug 21; 104-105:29-35. PMID: 32835810.
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  5. Gautier C, Troilo F, Cordier F, Malagrinò F, Toto A, Visconti L, Zhu Y, Brunori M, Wolff N, Gianni S. Hidden kinetic traps in multidomain folding highlight the presence of a misfolded but functionally competent intermediate. Proc Natl Acad Sci U S A. 2020 08 18; 117(33):19963-19969. PMID: 32747559.
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  6. Malagrinò F, Visconti L, Pagano L, Toto A, Troilo F, Gianni S. Understanding the Binding Induced Folding of Intrinsically Disordered Proteins by Protein Engineering: Caveats and Pitfalls. Int J Mol Sci. 2020 May 15; 21(10). PMID: 32429036.
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  7. Visconti L, Toto A, Jarvis JA, Troilo F, Malagrinò F, De Simone A, Gianni S. Demonstration of Binding Induced Structural Plasticity in a SH2 Domain. Front Mol Biosci. 2020; 7:89. PMID: 32528972.
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  8. Toto A, Malagrinò F, Visconti L, Troilo F, Pagano L, Brunori M, Jemth P, Gianni S. Templated folding of intrinsically disordered proteins. J Biol Chem. 2020 May 08; 295(19):6586-6593. PMID: 32253236.
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  9. Lei C, Su B, Dong H, Bellavia A, Di Fenza R, Safaee Fakhr B, Gianni S, Grassi LG, Kacmarek R, Araujo Morais CC, Pinciroli R, Vassena E, Berra L. Protocol of a randomized controlled trial testing inhaled Nitric Oxide in mechanically ventilated patients with severe acute respiratory syndrome in COVID-19 (SARS-CoV-2). medRxiv. 2020 Mar 13. PMID: 32511534.
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  10. Lei C, Su B, Dong H, Fakhr BS, Grassi LG, Di Fenza R, Gianni S, Pinciroli R, Vassena E, Morais CCA, Bellavia A, Spina S, Kacmarek R, Berra L. Protocol for a randomized controlled trial testing inhaled nitric oxide therapy in spontaneously breathing patients with COVID-19. medRxiv. 2020 Mar 13. PMID: 32511450.
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  11. Toto A, Malagrinò F, Visconti L, Troilo F, Gianni S. Unveiling the Molecular Basis of the Noonan Syndrome-Causing Mutation T42A of SHP2. Int J Mol Sci. 2020 Jan 10; 21(2). PMID: 31936901.
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  12. Toto A, Troilo F, Malagrinò F, Gianni S. Understanding Binding-Induced Folding by Temperature Jump. Methods Mol Biol. 2020; 2141:651-661. PMID: 32696382.
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  13. Gianni S, Jemth P. Affinity versus specificity in coupled binding and folding reactions. Protein Eng Des Sel. 2019 12 31; 32(8):355-357. PMID: 31397874.
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  14. Gianni S, Jemth P. Erratum to: Affinity versus specificity in coupled binding and folding reactions. Protein Eng Des Sel. 2019 12 31; 32(4):203. PMID: 31587044.
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  15. Troilo F, Malagrinò F, Visconti L, Toto A, Gianni S. The Effect of Proline cis-trans Isomerization on the Folding of the C-Terminal SH2 Domain from p85. Int J Mol Sci. 2019 Dec 23; 21(1). PMID: 31878075.
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  16. Laursen L, Karlsson E, Gianni S, Jemth P. Functional interplay between protein domains in a supramodular structure involving the postsynaptic density protein PSD-95. J Biol Chem. 2020 02 14; 295(7):1992-2000. PMID: 31831623.
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  17. Toto A, Troilo F, Visconti L, Malagrinò F, Bignon C, Longhi S, Gianni S. Binding induced folding: Lessons from the kinetics of interaction between NTAIL and XD. Arch Biochem Biophys. 2019 08 15; 671:255-261. PMID: 31326517.
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  18. Visconti L, Malagrinò F, Gianni S, Toto A. Structural characterization of an on-pathway intermediate and transition state in the folding of the N-terminal SH2 domain from SHP2. FEBS J. 2019 12; 286(23):4769-4777. PMID: 31287606.
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  19. Petrosino M, Pasquo A, Novak L, Toto A, Gianni S, Mantuano E, Veneziano L, Minicozzi V, Pastore A, Puglisi R, Capriotti E, Chiaraluce R, Consalvi V. Characterization of human frataxin missense variants in cancer tissues. Hum Mutat. 2019 09; 40(9):1400-1413. PMID: 31074541.
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  20. Malagrinò F, Troilo F, Bonetti D, Toto A, Gianni S. Mapping the allosteric network within a SH3 domain. Sci Rep. 2019 06 04; 9(1):8279. PMID: 31164678.
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  21. Visconti L, Malagrinò F, Toto A, Gianni S. The kinetics of folding of the NSH2 domain from p85. Sci Rep. 2019 03 11; 9(1):4058. PMID: 30858483.
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  22. Troilo F, Bonetti D, Bignon C, Longhi S, Gianni S. Understanding Intramolecular Crosstalk in an Intrinsically Disordered Protein. ACS Chem Biol. 2019 03 15; 14(3):337-341. PMID: 30715849.
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  23. Visconti L, Malagrinò F, Broggini L, De Luca CMG, Moda F, Gianni S, Ricagno S, Toto A. Investigating the Molecular Basis of the Aggregation Propensity of the Pathological D76N Mutant of Beta-2 Microglobulin: Role of the Denatured State. Int J Mol Sci. 2019 Jan 18; 20(2). PMID: 30669253.
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  24. Coluccia A, La Regina G, Naccarato V, Nalli M, Orlando V, Biagioni S, De Angelis ML, Baiocchi M, Gautier C, Gianni S, Di Pastena F, Di Magno L, Canettieri G, Coluccia AML, Silvestri R. Drug Design and Synthesis of First in Class PDZ1 Targeting NHERF1 Inhibitors as Anticancer Agents. ACS Med Chem Lett. 2019 Apr 11; 10(4):499-503. PMID: 30996786.
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  25. Bignon C, Troilo F, Gianni S, Longhi S. Modulation of Measles Virus NTAIL Interactions through Fuzziness and Sequence Features of Disordered Binding Sites. Biomolecules. 2018 12 27; 9(1). PMID: 30591682.
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  26. Karlsson E, Andersson E, Dogan J, Gianni S, Jemth P, Camilloni C. A structurally heterogeneous transition state underlies coupled binding and folding of disordered proteins. J Biol Chem. 2019 01 25; 294(4):1230-1239. PMID: 30514761.
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  27. Troilo F, Bignon C, Gianni S, Fuxreiter M, Longhi S. Experimental Characterization of Fuzzy Protein Assemblies: Interactions of Paramyxoviral NTAIL Domains With Their Functional Partners. Methods Enzymol. 2018; 611:137-192. PMID: 30471687.
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  28. Gautier C, Laursen L, Jemth P, Gianni S. Seeking allosteric networks in PDZ domains. Protein Eng Des Sel. 2018 10 01; 31(10):367-373. PMID: 30690500.
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  29. Gianni S, McCully ME, Malagrinò F, Bonetti D, De Simone A, Brunori M, Daggett V. A Carboxylate to Amide Substitution That Switches Protein Folds. Angew Chem Int Ed Engl. 2018 09 24; 57(39):12795-12798. PMID: 30098087.
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  30. Troilo F, Bonetti D, Camilloni C, Toto A, Longhi S, Brunori M, Gianni S. Folding Mechanism of the SH3 Domain from Grb2. J Phys Chem B. 2018 12 13; 122(49):11166-11173. PMID: 30091591.
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  31. Bonetti D, Troilo F, Toto A, Travaglini-Allocatelli C, Brunori M, Gianni S. Mechanism of Folding and Binding of the N-Terminal SH2 Domain from SHP2. J Phys Chem B. 2018 12 13; 122(49):11108-11114. PMID: 30047735.
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  32. Del Poggetto E, Toto A, Aloise C, Di Piro F, Gori L, Malatesta F, Gianni S, Chiti F, Bemporad F. Stability of an aggregation-prone partially folded state of human profilin-1 correlates with aggregation propensity. J Biol Chem. 2018 06 29; 293(26):10303-10313. PMID: 29760185.
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  33. Bonetti D, Troilo F, Brunori M, Longhi S, Gianni S. How Robust Is the Mechanism of Folding-Upon-Binding for an Intrinsically Disordered Protein? Biophys J. 2018 04 24; 114(8):1889-1894. PMID: 29694866.
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  34. Saponaro C, Sergio S, Coluccia A, De Luca M, La Regina G, Mologni L, Famiglini V, Naccarato V, Bonetti D, Gautier C, Gianni S, Vergara D, Salzet M, Fournier I, Bucci C, Silvestri R, Passerini CG, Maffia M, Coluccia AML. ß-catenin knockdown promotes NHERF1-mediated survival of colorectal cancer cells: implications for a double-targeted therapy. Oncogene. 2018 06; 37(24):3301-3316. PMID: 29551770.
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  35. Malgieri G, D'Abrosca G, Pirone L, Toto A, Palmieri M, Russo L, Sciacca MFM, Tatè R, Sivo V, Baglivo I, Majewska R, Coletta M, Pedone PV, Isernia C, De Stefano M, Gianni S, Pedone EM, Milardi D, Fattorusso R. Folding mechanisms steer the amyloid fibril formation propensity of highly homologous proteins. Chem Sci. 2018 Apr 07; 9(13):3290-3298. PMID: 29780459.
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  36. Caflisch A, Gianni S. Editorial overview: Folding and binding: In silico, in vitro and in cellula. Curr Opin Struct Biol. 2018 02; 48:iv-vii. PMID: 29475490.
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  37. Schramm A, Lieutaud P, Gianni S, Longhi S, Bignon C. InSiDDe: A Server for Designing Artificial Disordered Proteins. Int J Mol Sci. 2017 Dec 29; 19(1). PMID: 29286306.
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  38. Bignon C, Troilo F, Gianni S, Longhi S. Partner-Mediated Polymorphism of an Intrinsically Disordered Protein. J Mol Biol. 2018 08 03; 430(16):2493-2507. PMID: 29197511.
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  39. Gautier C, Visconti L, Jemth P, Gianni S. Addressing the role of the a-helical extension in the folding of the third PDZ domain from PSD-95. Sci Rep. 2017 10 03; 7(1):12593. PMID: 28974728.
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  40. Gianni S, Jemth P. How Fast Is Protein-Ligand Association? Trends Biochem Sci. 2017 11; 42(11):847-849. PMID: 28917971.
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  41. Caillet-Saguy C, Toto A, Guerois R, Maisonneuve P, di Silvio E, Sawyer K, Gianni S, Wolff N. Regulation of the Human Phosphatase PTPN4 by the inter-domain linker connecting the PDZ and the phosphatase domains. Sci Rep. 2017 08 11; 7(1):7875. PMID: 28801650.
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  42. Toto A, Bonetti D, De Simone A, Gianni S. Understanding the mechanism of binding between Gab2 and the C terminal SH3 domain from Grb2. Oncotarget. 2017 Oct 10; 8(47):82344-82351. PMID: 29137268.
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  43. Bonetti D, Troilo F, Toto A, Brunori M, Longhi S, Gianni S. Analyzing the Folding and Binding Steps of an Intrinsically Disordered Protein by Protein Engineering. Biochemistry. 2017 07 25; 56(29):3780-3786. PMID: 28661120.
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  44. Longhi S, Bloyet LM, Gianni S, Gerlier D. How order and disorder within paramyxoviral nucleoproteins and phosphoproteins orchestrate the molecular interplay of transcription and replication. Cell Mol Life Sci. 2017 09; 74(17):3091-3118. PMID: 28600653.
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  45. Landoni G, Lomivorotov V, Pisano A, Nigro Neto C, Benedetto U, Biondi Zoccai G, Gemma M, Frassoni S, Agrò FE, Baiocchi M, Barbosa Gomes Galas FR, Bautin A, Bradic N, Carollo C, Crescenzi G, Elnakera AM, El-Tahan MR, Fominskiy E, Farag AG, Gazivoda G, Gianni S, Grigoryev E, Guarracino F, Hanafi S, Huang W, Kunst G, Kunstyr J, Lei C, Lembo R, Li ZJ, Likhvantsev V, Lozovskiy A, Ma J, Monaco F, Navalesi P, Nazar B, Pasyuga V, Porteri E, Royse C, Ruggeri L, Riha H, Santos Silva F, Severi L, Shmyrev V, Uvaliev N, Wang CB, Wang CY, Winterton D, Yong CY, Yu J, Bellomo R, Zangrillo A. MortalitY in caRdIAc surgery (MYRIAD): A randomizeD controlled trial of volatile anesthetics. Rationale and design. Contemp Clin Trials. 2017 08; 59:38-43. PMID: 28533194.
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  46. Kukic P, Pustovalova Y, Camilloni C, Gianni S, Korzhnev DM, Vendruscolo M. Structural Characterization of the Early Events in the Nucleation-Condensation Mechanism in a Protein Folding Process. J Am Chem Soc. 2017 05 24; 139(20):6899-6910. PMID: 28401755.
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  47. Troilo F, Bonetti D, Toto A, Visconti L, Brunori M, Longhi S, Gianni S. The Folding Pathway of the KIX Domain. ACS Chem Biol. 2017 06 16; 12(6):1683-1690. PMID: 28459531.
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  48. Meroni R, Gianni S, Guarnieri M, Saglietti F, Gemma M, Zangrillo A, Bignami E. Feasibility of Anesthesia Maintenance With Sevoflurane During Cardiopulmonary Bypass: A Pilot Pharmacokinetics Study. J Cardiothorac Vasc Anesth. 2017 Aug; 31(4):1210-1217. PMID: 28283250.
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  49. Toto A, Mattei A, Jemth P, Gianni S. Understanding the role of phosphorylation in the binding mechanism of a PDZ domain. Protein Eng Des Sel. 2017 Jan; 30(1):1-5. PMID: 27760803.
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  50. Dogan J, Toto A, Andersson E, Gianni S, Jemth P. Activation Barrier-Limited Folding and Conformational Sampling of a Dynamic Protein Domain. Biochemistry. 2016 09 20; 55(37):5289-95. PMID: 27542287.
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  51. Camilloni C, Bonetti D, Morrone A, Giri R, Dobson CM, Brunori M, Gianni S, Vendruscolo M. Towards a structural biology of the hydrophobic effect in protein folding. Sci Rep. 2016 07 27; 6:28285. PMID: 27461719.
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  52. Toto A, Gianni S. Mutational Analysis of the Binding-Induced Folding Reaction of the Mixed-Lineage Leukemia Protein to the KIX Domain. Biochemistry. 2016 07 19; 55(28):3957-62. PMID: 27341615.
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  53. Brunori M, Gianni S. Molecular medicine - To be or not to be. Biophys Chem. 2016 Jul-Aug; 214-215:33-46. PMID: 27214761.
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  54. Zangrillo A, Alvaro G, Pisano A, Guarracino F, Lobreglio R, Bradic N, Lembo R, Gianni S, Calabrò MG, Likhvantsev V, Grigoryev E, Buscaglia G, Pala G, Auci E, Amantea B, Monaco F, De Vuono G, Corcione A, Galdieri N, Cariello C, Bove T, Fominskiy E, Auriemma S, Baiocchi M, Bianchi A, Frontini M, Paternoster G, Sangalli F, Wang CY, Zucchetti MC, Biondi-Zoccai G, Gemma M, Lipinski MJ, Lomivorotov VV, Landoni G. A randomized controlled trial of levosimendan to reduce mortality in high-risk cardiac surgery patients (CHEETAH): Rationale and design. Am Heart J. 2016 Jul; 177:66-73. PMID: 27297851.
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  55. Bonetti D, Camilloni C, Visconti L, Longhi S, Brunori M, Vendruscolo M, Gianni S. Identification and Structural Characterization of an Intermediate in the Folding of the Measles Virus X Domain. J Biol Chem. 2016 May 13; 291(20):10886-92. PMID: 27002146.
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  56. Gianni S, Jemth P. Protein folding: Vexing debates on a fundamental problem. Biophys Chem. 2016 May; 212:17-21. PMID: 27018826.
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  57. Toto A, Pedersen SW, Karlsson OA, Moran GE, Andersson E, Chi CN, Strømgaard K, Gianni S, Jemth P. Ligand binding to the PDZ domains of postsynaptic density protein 95. Protein Eng Des Sel. 2016 May; 29(5):169-75. PMID: 26941280.
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  58. Toto A, Camilloni C, Giri R, Brunori M, Vendruscolo M, Gianni S. Molecular Recognition by Templated Folding of an Intrinsically Disordered Protein. Sci Rep. 2016 Feb 25; 6:21994. PMID: 26912067.
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  59. Bolognesi C, Forcato C, Buson G, Fontana F, Mangano C, Doffini A, Sero V, Lanzellotto R, Signorini G, Calanca A, Sergio M, Romano R, Gianni S, Medoro G, Giorgini G, Morreau H, Barberis M, Corver WE, Manaresi N. Digital Sorting of Pure Cell Populations Enables Unambiguous Genetic Analysis of Heterogeneous Formalin-Fixed Paraffin-Embedded Tumors by Next Generation Sequencing. Sci Rep. 2016 Feb 11; 6:20944. PMID: 26864208.
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  60. Gruet A, Dosnon M, Blocquel D, Brunel J, Gerlier D, Das RK, Bonetti D, Gianni S, Fuxreiter M, Longhi S, Bignon C. Fuzzy regions in an intrinsically disordered protein impair protein-protein interactions. FEBS J. 2016 Feb; 283(4):576-94. PMID: 26684000.
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  61. Gianni S, Dogan J, Jemth P. Coupled binding and folding of intrinsically disordered proteins: what can we learn from kinetics? Curr Opin Struct Biol. 2016 Feb; 36:18-24. PMID: 26720267.
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  62. Di Silvio E, Brunori M, Gianni S. Frustration Sculpts the Early Stages of Protein Folding. Angew Chem Int Ed Engl. 2015 Sep 07; 54(37):10867-9. PMID: 26212018.
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  63. Di Silvio E, Toto A, Bonetti D, Morrone A, Gianni S. Understanding the effect of alternative splicing in the folding and function of the second PDZ from protein tyrosine phosphatase-BL. Sci Rep. 2015 Mar 19; 5:9299. PMID: 25788329.
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  64. Dosnon M, Bonetti D, Morrone A, Erales J, di Silvio E, Longhi S, Gianni S. Demonstration of a folding after binding mechanism in the recognition between the measles virus NTAIL and X domains. ACS Chem Biol. 2015 Mar 20; 10(3):795-802. PMID: 25511246.
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  65. Gianni S, Dogan J, Jemth P. Deciphering the mechanisms of binding induced folding at nearly atomic resolution: The F value analysis applied to IDPs. Intrinsically Disord Proteins. 2014; 2(1):e970900. PMID: 28232881.
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  66. Polzer B, Medoro G, Pasch S, Fontana F, Zorzino L, Pestka A, Andergassen U, Meier-Stiegen F, Czyz ZT, Alberter B, Treitschke S, Schamberger T, Sergio M, Bregola G, Doffini A, Gianni S, Calanca A, Signorini G, Bolognesi C, Hartmann A, Fasching PA, Sandri MT, Rack B, Fehm T, Giorgini G, Manaresi N, Klein CA. Molecular profiling of single circulating tumor cells with diagnostic intention. EMBO Mol Med. 2014 Nov; 6(11):1371-86. PMID: 25358515.
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  67. Gianni S, Camilloni C, Giri R, Toto A, Bonetti D, Morrone A, Sormanni P, Brunori M, Vendruscolo M. Understanding the frustration arising from the competition between function, misfolding, and aggregation in a globular protein. Proc Natl Acad Sci U S A. 2014 Sep 30; 111(39):14141-6. PMID: 25228761.
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  68. Gianni S, Jemth P. Conserved nucleation sites reinforce the significance of Phi value analysis in protein-folding studies. IUBMB Life. 2014 Jul; 66(7):449-52. PMID: 25044918.
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  69. Conti S, Li X, Gianni S, Ghadami SA, Buxbaum J, Cecchi C, Chiti F, Bemporad F. A complex equilibrium among partially unfolded conformations in monomeric transthyretin. Biochemistry. 2014 Jul 15; 53(27):4381-92. PMID: 24945718.
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  70. Di Silvio E, Bonetti D, Toto A, Morrone A, Gianni S. The mechanism of binding of the second PDZ domain from the Protein Tyrosine Phosphatase-BL to the Adenomatous Polyposis Coli tumor suppressor. Protein Eng Des Sel. 2014 Aug; 27(8):249-53. PMID: 24928580.
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  71. Toto A, Giri R, Brunori M, Gianni S. The mechanism of binding of the KIX domain to the mixed lineage leukemia protein and its allosteric role in the recognition of c-Myb. Protein Sci. 2014 Jul; 23(7):962-9. PMID: 24753318.
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  72. Gianni S, Dogan J, Jemth P. Distinguishing induced fit from conformational selection. Biophys Chem. 2014 May; 189:33-9. PMID: 24747333.
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  73. Bonetti D, Toto A, Giri R, Morrone A, Sanfelice D, Pastore A, Temussi P, Gianni S, Brunori M. The kinetics of folding of frataxin. Phys Chem Chem Phys. 2014 Apr 14; 16(14):6391-7. PMID: 24429875.
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  74. Dogan J, Gianni S, Jemth P. The binding mechanisms of intrinsically disordered proteins. Phys Chem Chem Phys. 2014 Apr 14; 16(14):6323-31. PMID: 24317797.
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  75. Ascenzi P, Gianni S. Functional role of transient conformations: Rediscovering "chronosteric effects" thirty years later. IUBMB Life. 2013 Oct; 65(10):836-44. PMID: 24078391.
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  76. Giri R, Morrone A, Toto A, Brunori M, Gianni S. Structure of the transition state for the binding of c-Myb and KIX highlights an unexpected order for a disordered system. Proc Natl Acad Sci U S A. 2013 Sep 10; 110(37):14942-7. PMID: 23980173.
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  77. Hultqvist G, Haq SR, Punekar AS, Chi CN, Engström Å, Bach A, Strømgaard K, Selmer M, Gianni S, Jemth P. Energetic pathway sampling in a protein interaction domain. Structure. 2013 Jul 02; 21(7):1193-1202. PMID: 23810696.
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  78. Eildal JN, Hultqvist G, Balle T, Stuhr-Hansen N, Padrah S, Gianni S, Strømgaard K, Jemth P. Probing the role of backbone hydrogen bonds in protein-peptide interactions by amide-to-ester mutations. J Am Chem Soc. 2013 Sep 04; 135(35):12998-3007. PMID: 23705582.
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  79. Chiarella S, Federici L, Di Matteo A, Brunori M, Gianni S. The folding pathway of a functionally competent C-terminal domain of nucleophosmin: protein stability and denatured state residual structure. Biochem Biophys Res Commun. 2013 May 24; 435(1):64-8. PMID: 23618861.
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  80. Hultqvist G, Punekar AS, Morrone A, Chi CN, Engström A, Selmer M, Gianni S, Jemth P. Tolerance of protein folding to a circular permutation in a PDZ domain. PLoS One. 2012; 7(11):e50055. PMID: 23185531.
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  81. Polticelli F, Caprari S, Gianni S, Ascenzi P. GA/GB fold switching may modulate fatty acid transfer from human serum albumin to bacteria. IUBMB Life. 2012 Nov; 64(11):885-8. PMID: 23086813.
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  82. Chi CN, Haq SR, Rinaldo S, Dogan J, Cutruzzolà F, Engström Å, Gianni S, Lundström P, Jemth P. Interactions outside the boundaries of the canonical binding groove of a PDZ domain influence ligand binding. Biochemistry. 2012 Nov 06; 51(44):8971-9. PMID: 23046383.
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  83. Morrone A, Giri R, Brunori M, Gianni S. Reassessing the folding of the KIX domain: evidence for a two-state mechanism. Protein Sci. 2012 Nov; 21(11):1775-9. PMID: 23011783.
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  84. Gianni S, Morrone A, Giri R, Brunori M. A folding-after-binding mechanism describes the recognition between the transactivation domain of c-Myb and the KIX domain of the CREB-binding protein. Biochem Biophys Res Commun. 2012 Nov 16; 428(2):205-9. PMID: 23026051.
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  85. Chi CN, Bach A, Strømgaard K, Gianni S, Jemth P. Ligand binding by PDZ domains. Biofactors. 2012 Sep-Oct; 38(5):338-48. PMID: 22674855.
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  86. Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S. Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A. 2012 Oct 30; 109(44):17772-6. PMID: 22652570.
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  87. Hultqvist G, Pedersen SW, Chi CN, Strømgaard K, Gianni S, Jemth P. An expanded view of the protein folding landscape of PDZ domains. Biochem Biophys Res Commun. 2012 May 11; 421(3):550-3. PMID: 22521641.
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  88. Brunori M, Gianni S, Giri R, Morrone A, Travaglini-Allocatelli C. Morphogenesis of a protein: folding pathways and the energy landscape. Biochem Soc Trans. 2012 Apr; 40(2):429-32. PMID: 22435825.
    Citations:    
  89. Haq SR, Chi CN, Bach A, Dogan J, Engström Å, Hultqvist G, Karlsson OA, Lundström P, Montemiglio LC, Strømgaard K, Gianni S, Jemth P. Side-chain interactions form late and cooperatively in the binding reaction between disordered peptides and PDZ domains. J Am Chem Soc. 2012 Jan 11; 134(1):599-605. PMID: 22129097.
    Citations:    
  90. Giardina G, Montioli R, Gianni S, Cellini B, Paiardini A, Voltattorni CB, Cutruzzolà F. Open conformation of human DOPA decarboxylase reveals the mechanism of PLP addition to Group II decarboxylases. Proc Natl Acad Sci U S A. 2011 Dec 20; 108(51):20514-9. PMID: 22143761.
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  91. Morrone A, Giri R, Toofanny RD, Travaglini-Allocatelli C, Brunori M, Daggett V, Gianni S. GB1 is not a two-state folder: identification and characterization of an on-pathway intermediate. Biophys J. 2011 Oct 19; 101(8):2053-60. PMID: 22004760.
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  92. Gianni S, Haq SR, Montemiglio LC, Jürgens MC, Engström Å, Chi CN, Brunori M, Jemth P. Sequence-specific long range networks in PSD-95/discs large/ZO-1 (PDZ) domains tune their binding selectivity. J Biol Chem. 2011 Aug 05; 286(31):27167-75. PMID: 21653701.
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  93. Morrone A, McCully ME, Bryan PN, Brunori M, Daggett V, Gianni S, Travaglini-Allocatelli C. The denatured state dictates the topology of two proteins with almost identical sequence but different native structure and function. J Biol Chem. 2011 Feb 04; 286(5):3863-72. PMID: 21118804.
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  94. Gianni S, Ivarsson Y, De Simone A, Travaglini-Allocatelli C, Brunori M, Vendruscolo M. Structural characterization of a misfolded intermediate populated during the folding process of a PDZ domain. Nat Struct Mol Biol. 2010 Dec; 17(12):1431-7. PMID: 21076399.
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  95. Montemiglio LC, Gianni S, Vallone B, Savino C. Azole drugs trap cytochrome P450 EryK in alternative conformational states. Biochemistry. 2010 Nov 02; 49(43):9199-206. PMID: 20845962.
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  96. Di Matteo A, Calosci N, Gianni S, Jemth P, Brunori M, Travaglini-Allocatelli C. Structural and functional characterization of CcmG from Pseudomonas aeruginosa, a key component of the bacterial cytochrome c maturation apparatus. Proteins. 2010 Aug 01; 78(10):2213-21. PMID: 20544959.
    Citations:    
  97. Haq SR, Jürgens MC, Chi CN, Koh CS, Elfström L, Selmer M, Gianni S, Jemth P. The plastic energy landscape of protein folding: a triangular folding mechanism with an equilibrium intermediate for a small protein domain. J Biol Chem. 2010 Jun 04; 285(23):18051-9. PMID: 20356847.
    Citations:    
  98. Scaloni F, Federici L, Brunori M, Gianni S. Deciphering the folding transition state structure and denatured state properties of nucleophosmin C-terminal domain. Proc Natl Acad Sci U S A. 2010 Mar 23; 107(12):5447-52. PMID: 20212148.
    Citations:    
  99. Gianni S, Brunori M, Jemth P, Oliveberg M, Zhang M. Distinguishing between smooth and rough free energy barriers in protein folding. Biochemistry. 2009 Dec 15; 48(49):11825-30. PMID: 19877713.
    Citations:    
  100. Giansanti F, Di Leandro L, Koutris I, Pitari G, Fabbrini MS, Lombardi A, Flavell DJ, Flavell SU, Gianni S, Ippoliti R. Engineering a switchable toxin: the potential use of PDZ domains in the expression, targeting and activation of modified saporin variants. Protein Eng Des Sel. 2010 Feb; 23(2):61-8. PMID: 19933699.
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  101. Ivarsson Y, Travaglini-Allocatelli C, Brunori M, Gianni S. Engineered symmetric connectivity of secondary structure elements highlights malleability of protein folding pathways. J Am Chem Soc. 2009 Aug 26; 131(33):11727-33. PMID: 19722594.
    Citations:    
  102. Chi CN, Bach A, Engström A, Wang H, Strømgaard K, Gianni S, Jemth P. A sequential binding mechanism in a PDZ domain. Biochemistry. 2009 Aug 04; 48(30):7089-97. PMID: 19496620.
    Citations:    
  103. Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B. Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate. J Biol Chem. 2009 Oct 16; 284(42):29170-9. PMID: 19625248.
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  104. Sicorello A, Torrassa S, Soldi G, Gianni S, Travaglini-Allocatelli C, Taddei N, Relini A, Chiti F. Agitation and high ionic strength induce amyloidogenesis of a folded PDZ domain in native conditions. Biophys J. 2009 Mar 18; 96(6):2289-98. PMID: 19289055.
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  105. Scaloni F, Gianni S, Federici L, Falini B, Brunori M. Folding mechanism of the C-terminal domain of nucleophosmin: residual structure in the denatured state and its pathophysiological significance. FASEB J. 2009 Aug; 23(8):2360-5. PMID: 19289604.
    Citations:    
  106. Federici L, Masulli M, Gianni S, Di Ilio C, Allocati N. A conserved hydrogen-bond network stabilizes the structure of Beta class glutathione S-transferases. Biochem Biophys Res Commun. 2009 May 08; 382(3):525-9. PMID: 19289099.
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  107. Travaglini-Allocatelli C, Ivarsson Y, Jemth P, Gianni S. Folding and stability of globular proteins and implications for function. Curr Opin Struct Biol. 2009 Feb; 19(1):3-7. PMID: 19157852.
    Citations:    
  108. Calosci N, Chi CN, Richter B, Camilloni C, Engström A, Eklund L, Travaglini-Allocatelli C, Gianni S, Vendruscolo M, Jemth P. Comparison of successive transition states for folding reveals alternative early folding pathways of two homologous proteins. Proc Natl Acad Sci U S A. 2008 Dec 09; 105(49):19241-6. PMID: 19033470.
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  109. Parrini C, Bemporad F, Baroncelli A, Gianni S, Travaglini-Allocatelli C, Kohn JE, Ramazzotti M, Chiti F, Taddei N. The folding process of acylphosphatase from Escherichia coli is remarkably accelerated by the presence of a disulfide bond. J Mol Biol. 2008 Jun 20; 379(5):1107-18. PMID: 18495159.
    Citations:    
  110. Borgia A, Gianni S, Brunori M, Travaglini-Allocatelli C. Fast folding kinetics and stabilization of apo-cytochrome c. FEBS Lett. 2008 Mar 19; 582(6):1003-7. PMID: 18307988.
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  111. Ivarsson Y, Travaglini-Allocatelli C, Brunori M, Gianni S. Folding and misfolding in a naturally occurring circularly permuted PDZ domain. J Biol Chem. 2008 Apr 04; 283(14):8954-60. PMID: 18263589.
    Citations:    
  112. Jemth P, Johnson CM, Gianni S, Fersht AR. Demonstration by burst-phase analysis of a robust folding intermediate in the FF domain. Protein Eng Des Sel. 2008 Mar; 21(3):207-14. PMID: 18239073.
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  113. Ivarsson Y, Travaglini-Allocatelli C, Brunori M, Gianni S. Mechanisms of protein folding. Eur Biophys J. 2008 Jul; 37(6):721-8. PMID: 18185928.
    Citations:    
  114. Ivarsson Y, Travaglini-Allocatelli C, Morea V, Brunori M, Gianni S. The folding pathway of an engineered circularly permuted PDZ domain. Protein Eng Des Sel. 2008 Mar; 21(3):155-60. PMID: 18175779.
    Citations:    
  115. Gianni S, Ivarsson Y, Bah A, Bush-Pelc LA, Di Cera E. Mechanism of Na(+) binding to thrombin resolved by ultra-rapid kinetics. Biophys Chem. 2007 Dec; 131(1-3):111-4. PMID: 17935858.
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  116. Jemth P, Gianni S. PDZ domains: folding and binding. Biochemistry. 2007 Jul 31; 46(30):8701-8. PMID: 17620015.
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  117. Di Matteo A, Gianni S, Schininà ME, Giorgi A, Altieri F, Calosci N, Brunori M, Travaglini-Allocatelli C. A strategic protein in cytochrome c maturation: three-dimensional structure of CcmH and binding to apocytochrome c. J Biol Chem. 2007 Sep 14; 282(37):27012-9. PMID: 17623665.
    Citations:    
  118. Gianni S, Brunori M, Travaglini-Allocatelli C. Plasticity of the protein folding landscape: switching between on- and off-pathway intermediates. Arch Biochem Biophys. 2007 Oct 15; 466(2):172-6. PMID: 17658452.
    Citations:    
  119. Gianni S, Ivarsson Y, Jemth P, Brunori M, Travaglini-Allocatelli C. Identification and characterization of protein folding intermediates. Biophys Chem. 2007 Jul; 128(2-3):105-13. PMID: 17498862.
    Citations:    
  120. Federici L, Masulli M, Bonivento D, Di Matteo A, Gianni S, Favaloro B, Di Ilio C, Allocati N. Role of Ser11 in the stabilization of the structure of Ochrobactrum anthropi glutathione transferase. Biochem J. 2007 Apr 15; 403(2):267-74. PMID: 17223798.
    Citations:    
  121. Chi CN, Gianni S, Calosci N, Travaglini-Allocatelli C, Engström K, Jemth P. A conserved folding mechanism for PDZ domains. FEBS Lett. 2007 Mar 20; 581(6):1109-13. PMID: 17316619.
    Citations:    
  122. Ivarsson Y, Travaglini-Allocatelli C, Jemth P, Malatesta F, Brunori M, Gianni S. An on-pathway intermediate in the folding of a PDZ domain. J Biol Chem. 2007 Mar 23; 282(12):8568-72. PMID: 17251182.
    Citations:    
  123. Gianni S, Geierhaas CD, Calosci N, Jemth P, Vuister GW, Travaglini-Allocatelli C, Vendruscolo M, Brunori M. A PDZ domain recapitulates a unifying mechanism for protein folding. Proc Natl Acad Sci U S A. 2007 Jan 02; 104(1):128-33. PMID: 17179214.
    Citations:    
  124. Gianni S, Walma T, Arcovito A, Calosci N, Bellelli A, Engström A, Travaglini-Allocatelli C, Brunori M, Jemth P, Vuister GW. Demonstration of long-range interactions in a PDZ domain by NMR, kinetics, and protein engineering. Structure. 2006 Dec; 14(12):1801-9. PMID: 17161370.
    Citations:    
  125. Chi CN, Engström A, Gianni S, Larsson M, Jemth P. Two conserved residues govern the salt and pH dependencies of the binding reaction of a PDZ domain. J Biol Chem. 2006 Dec 01; 281(48):36811-8. PMID: 17018532.
    Citations:    
  126. Gianni S, Engström A, Larsson M, Calosci N, Malatesta F, Eklund L, Ngang CC, Travaglini-Allocatelli C, Jemth P. The kinetics of PDZ domain-ligand interactions and implications for the binding mechanism. J Biol Chem. 2005 Oct 14; 280(41):34805-12. PMID: 16049001.
    Citations:    
  127. Gianni S, Calosci N, Aelen JM, Vuister GW, Brunori M, Travaglini-Allocatelli C. Kinetic folding mechanism of PDZ2 from PTP-BL. Protein Eng Des Sel. 2005 Aug; 18(8):389-95. PMID: 16043447.
    Citations:    
  128. White GW, Gianni S, Grossmann JG, Jemth P, Fersht AR, Daggett V. Simulation and experiment conspire to reveal cryptic intermediates and a slide from the nucleation-condensation to framework mechanism of folding. J Mol Biol. 2005 Jul 22; 350(4):757-75. PMID: 15967458.
    Citations:    
  129. Jemth P, Day R, Gianni S, Khan F, Allen M, Daggett V, Fersht AR. The structure of the major transition state for folding of an FF domain from experiment and simulation. J Mol Biol. 2005 Jul 08; 350(2):363-78. PMID: 15935381.
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  130. Travaglini-Allocatelli C, Gianni S, Dubey VK, Borgia A, Di Matteo A, Bonivento D, Cutruzzolà F, Bren KL, Brunori M. An obligatory intermediate in the folding pathway of cytochrome c552 from Hydrogenobacter thermophilus. J Biol Chem. 2005 Jul 08; 280(27):25729-34. PMID: 15883159.
    Citations:    
  131. Travaglini-Allocatelli C, Gianni S, Brunori M. A common folding mechanism in the cytochrome c family. Trends Biochem Sci. 2004 Oct; 29(10):535-41. PMID: 15450608.
    Citations:    
  132. Jemth P, Gianni S, Day R, Li B, Johnson CM, Daggett V, Fersht AR. Demonstration of a low-energy on-pathway intermediate in a fast-folding protein by kinetics, protein engineering, and simulation. Proc Natl Acad Sci U S A. 2004 Apr 27; 101(17):6450-5. PMID: 15096617.
    Citations:    
  133. Gianni S, Mayor U, Fersht AR. Structural insights in the folding of small single-domain proteins. Ital J Biochem. 2003 Dec; 52(4):154-61. PMID: 15141482.
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  134. Gianni S, Guydosh NR, Khan F, Caldas TD, Mayor U, White GW, DeMarco ML, Daggett V, Fersht AR. Unifying features in protein-folding mechanisms. Proc Natl Acad Sci U S A. 2003 Nov 11; 100(23):13286-91. PMID: 14595026.
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  135. Khan F, Chuang JI, Gianni S, Fersht AR. The kinetic pathway of folding of barnase. J Mol Biol. 2003 Oct 10; 333(1):169-86. PMID: 14516751.
    Citations:    
  136. Gianni S, Travaglini-Allocatelli C, Cutruzzolà F, Brunori M, Shastry MC, Roder H. Parallel pathways in cytochrome c(551) folding. J Mol Biol. 2003 Jul 25; 330(5):1145-52. PMID: 12860134.
    Citations:    
  137. Travaglini-Allocatelli C, Gianni S, Morea V, Tramontano A, Soulimane T, Brunori M. Exploring the cytochrome c folding mechanism: cytochrome c552 from thermus thermophilus folds through an on-pathway intermediate. J Biol Chem. 2003 Oct 17; 278(42):41136-40. PMID: 12842869.
    Citations:    
  138. Brunori M, Bigotti MG, Cutruzzolà F, Gianni S, Travaglini-Allocatelli C. Cytochrome c(551) as a model system for protein folding. Biophys Chem. 2003; 100(1-3):409-19. PMID: 12646380.
    Citations:    
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.