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John R. Engen, Ph.D.

Title
Institution
Department
Address

Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R01CA233978 (ENGEN, JOHN R) Dec 1, 2019 - Nov 30, 2024
    NIH/NCI
    Conformational Regulation and Therapeutic Targeting of Oncogenic KRAS
    Role: Principal Investigator
  2. R01GM101135 (ENGEN, JOHN R) Sep 30, 2012 - Jul 31, 2016
    NIH/NIGMS
    Extending the applications of hydrogen exchange mass spectrometry
    Role: Principal Investigator
  3. R01GM086507 (ENGEN, JOHN R) Sep 30, 2009 - Aug 31, 2014
    NIH/NIGMS
    Protein conformational change upon membrane association
    Role: Principal Investigator
  4. R01GM070590 (ENGEN, JOHN R) Apr 1, 2004 - Mar 31, 2010
    NIH/NIGMS
    Structural Dynamics of Src-Family Kinase Activation
    Role: Principal Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Bird GH, Fu A, Escudero S, Godes M, Opoku-Nsiah K, Wales TE, Cameron MD, Engen JR, Danial NN, Walensky LD. Hydrocarbon-stitched Peptide Agonists of Glucagon-Like Peptide-1 Receptor. ACS Chem Biol. 2020 Apr 29. PMID: 32348108.
    Citations:    
  2. Ben-Nun Y, Seo HS, Harvey EP, Hauseman ZJ, Wales TE, Newman CE, Cathcart AM, Engen JR, Dhe-Paganon S, Walensky LD. Identification of a Structural Determinant for Selective Targeting of HDMX. Structure. 2020 Apr 29. PMID: 32359398.
    Citations:    
  3. Harvey EP, Hauseman ZJ, Cohen DT, Rettenmaier TJ, Lee S, Huhn AJ, Wales TE, Seo HS, Luccarelli J, Newman CE, Guerra RM, Bird GH, Dhe-Paganon S, Engen JR, Wells JA, Walensky LD. Identification of a Covalent Molecular Inhibitor of Anti-apoptotic BFL-1 by Disulfide Tethering. Cell Chem Biol. 2020 Apr 21. PMID: 32413285.
    Citations:    
  4. Schauer NJ, Liu X, Magin RS, Doherty LM, Chan WC, Ficarro SB, Hu W, Roberts RM, Iacob RE, Stolte B, Giacomelli AO, Perera S, McKay K, Boswell SA, Weisberg EL, Ray A, Chauhan D, Dhe-Paganon S, Anderson KC, Griffin JD, Li J, Hahn WC, Sorger PK, Engen JR, Stegmaier K, Marto JA, Buhrlage SJ. Selective USP7 inhibition elicits cancer cell killing through a p53-dependent mechanism. Sci Rep. 2020 Mar 24; 10(1):5324. PMID: 32210275.
    Citations:    
  5. Cohen DT, Wales TE, McHenry MW, Engen JR, Walensky LD. Site-Dependent Cysteine Lipidation Potentiates the Activation of Proapoptotic BAX. Cell Rep. 2020 Mar 10; 30(10):3229-3239.e6. PMID: 32160532.
    Citations:    
  6. Fleischhacker AS, Gunawan AL, Kochert BA, Liu L, Wales TE, Borowy MC, Engen JR, Ragsdale SW. The heme-regulatory motifs of heme oxygenase-2 contribute to the transfer of heme to the catalytic site for degradation. J Biol Chem. 2020 Apr 17; 295(16):5177-5191. PMID: 32152224.
    Citations:    
  7. Kardon JR, Moroco JA, Engen JR, Baker TA. Mitochondrial ClpX activates an essential biosynthetic enzyme through partial unfolding. Elife. 2020 Feb 24; 9. PMID: 32091391.
    Citations:    
  8. Frame NM, Kumanan M, Wales TE, Bandara A, Fändrich M, Straub JE, Engen JR, Gursky O. Structural Basis for Lipid Binding and Function by an Evolutionarily Conserved Protein, Serum Amyloid A. J Mol Biol. 2020 Mar 27; 432(7):1978-1995. PMID: 32035904.
    Citations:    
  9. Makarov AA, Iacob RE, Pirrone GF, Rodriguez-Granillo A, Joyce L, Mangion I, Moore JC, Sherer EC, Engen JR. Combination of HDX-MS and in silico modeling to study enzymatic reactivity and stereo-selectivity at different solvent conditions. J Pharm Biomed Anal. 2020 Apr 15; 182:113141. PMID: 32036298.
    Citations:    
  10. Manthei KA, Patra D, Wilson CJ, Fawaz MV, Piersimoni L, Shenkar JC, Yuan W, Andrews PC, Engen JR, Schwendeman A, Ohi MD, Tesmer JJG. Structural analysis of lecithin:cholesterol acyltransferase bound to high density lipoprotein particles. Commun Biol. 2020 Jan 15; 3(1):28. PMID: 31942029.
    Citations:    
  11. Eggertson MJ, Fadgen K, Engen JR, Wales TE. Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data. Methods Mol Biol. 2020; 2051:407-435. PMID: 31552640.
    Citations:    
  12. Wang J, Su Y, Iacob RE, Engen JR, Springer TA. General structural features that regulate integrin affinity revealed by atypical aVß8. Nat Commun. 2019 12 02; 10(1):5481. PMID: 31792290.
    Citations:    
  13. Amatya N, Wales TE, Kwon A, Yeung W, Joseph RE, Fulton DB, Kannan N, Engen JR, Andreotti AH. Lipid-targeting pleckstrin homology domain turns its autoinhibitory face toward the TEC kinases. Proc Natl Acad Sci U S A. 2019 10 22; 116(43):21539-21544. PMID: 31591208.
    Citations:    
  14. Mourtada R, Herce HD, Yin DJ, Moroco JA, Wales TE, Engen JR, Walensky LD. Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice. Nat Biotechnol. 2019 10; 37(10):1186-1197. PMID: 31427820.
    Citations:    
  15. Bera AK, Lu J, Wales TE, Gondi S, Gurbani D, Nelson A, Engen JR, Westover KD. Structural basis of the atypical activation mechanism of KRASV14I. J Biol Chem. 2019 09 20; 294(38):13964-13972. PMID: 31341022.
    Citations:    
  16. Masson GR, Burke JE, Ahn NG, Anand GS, Borchers C, Brier S, Bou-Assaf GM, Engen JR, Englander SW, Faber J, Garlish R, Griffin PR, Gross ML, Guttman M, Hamuro Y, Heck AJR, Houde D, Iacob RE, Jørgensen TJD, Kaltashov IA, Klinman JP, Konermann L, Man P, Mayne L, Pascal BD, Reichmann D, Skehel M, Snijder J, Strutzenberg TS, Underbakke ES, Wagner C, Wales TE, Walters BT, Weis DD, Wilson DJ, Wintrode PL, Zhang Z, Zheng J, Schriemer DC, Rand KD. Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nat Methods. 2019 07; 16(7):595-602. PMID: 31249422.
    Citations:    
  17. Twomey EC, Ji Z, Wales TE, Bodnar NO, Ficarro SB, Marto JA, Engen JR, Rapoport TA. Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding. Science. 2019 08 02; 365(6452). PMID: 31249135.
    Citations:    
  18. Georgescauld F, Wales TE, Engen JR. Hydrogen deuterium exchange mass spectrometry applied to chaperones and chaperone-assisted protein folding. Expert Rev Proteomics. 2019 07; 16(7):613-625. PMID: 31215268.
    Citations:    
  19. Poulin EJ, Bera AK, Lu J, Lin YJ, Strasser SD, Paulo JA, Huang TQ, Morales C, Yan W, Cook J, Nowak JA, Brubaker DK, Joughin BA, Johnson CW, DeStefanis RA, Ghazi PC, Gondi S, Wales TE, Iacob RE, Bogdanova L, Gierut JJ, Li Y, Engen JR, Perez-Mancera PA, Braun BS, Gygi SP, Lauffenburger DA, Westover KD, Haigis KM. Tissue-Specific Oncogenic Activity of KRASA146T. Cancer Discov. 2019 06; 9(6):738-755. PMID: 30952657.
    Citations:    
  20. Kochert BA, Fleischhacker AS, Wales TE, Becker DF, Engen JR, Ragsdale SW. Dynamic and structural differences between heme oxygenase-1 and -2 are due to differences in their C-terminal regions. J Biol Chem. 2019 05 17; 294(20):8259-8272. PMID: 30944174.
    Citations:    
  21. Dautant A, Henri J, Wales TE, Meyer P, Engen JR, Georgescauld F. Remodeling of the Binding Site of Nucleoside Diphosphate Kinase Revealed by X-ray Structure and H/D Exchange. Biochemistry. 2019 03 12; 58(10):1440-1449. PMID: 30785730.
    Citations:    
  22. Shen K, Moroco JA, Patel RK, Shi H, Engen JR, Dorman HR, Smithgall TE. The Src family kinase Fgr is a transforming oncoprotein that functions independently of SH3-SH2 domain regulation. Sci Signal. 2018 10 23; 11(553). PMID: 30352950.
    Citations:    
  23. Feng J, Dong X, Pinello J, Zhang J, Lu C, Iacob RE, Engen JR, Snell WJ, Springer TA. Fusion surface structure, function, and dynamics of gamete fusogen HAP2. Elife. 2018 10 03; 7. PMID: 30281023.
    Citations:    Fields:    
  24. Wilson CJ, Das M, Jayaraman S, Gursky O, Engen JR. Effects of Disease-Causing Mutations on the Conformation of Human Apolipoprotein A-I in Model Lipoproteins. Biochemistry. 2018 07 31; 57(30):4583-4596. PMID: 30004693.
    Citations:    
  25. Bodnar NO, Kim KH, Ji Z, Wales TE, Svetlov V, Nudler E, Engen JR, Walz T, Rapoport TA. Structure of the Cdc48 ATPase with its ubiquitin-binding cofactor Ufd1-Npl4. Nat Struct Mol Biol. 2018 07; 25(7):616-622. PMID: 29967539.
    Citations:    Fields:    
  26. de Wispelaere M, Lian W, Potisopon S, Li PC, Jang J, Ficarro SB, Clark MJ, Zhu X, Kaplan JB, Pitts JD, Wales TE, Wang J, Engen JR, Marto JA, Gray NS, Yang PL. Inhibition of Flaviviruses by Targeting a Conserved Pocket on the Viral Envelope Protein. Cell Chem Biol. 2018 08 16; 25(8):1006-1016.e8. PMID: 29937406.
    Citations: 1     Fields:    
  27. Escudero S, Zaganjor E, Lee S, Mill CP, Morgan AM, Crawford EB, Chen J, Wales TE, Mourtada R, Luccarelli J, Bird GH, Steidl U, Engen JR, Haigis MC, Opferman JT, Walensky LD. Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD. Mol Cell. 2018 03 01; 69(5):729-743.e7. PMID: 29499131.
    Citations: 1     Fields:    Translation:AnimalsCells
  28. Le VQ, Iacob RE, Tian Y, McConaughy W, Jackson J, Su Y, Zhao B, Engen JR, Pirruccello-Straub M, Springer TA. Tolloid cleavage activates latent GDF8 by priming the pro-complex for dissociation. EMBO J. 2018 02 01; 37(3):384-397. PMID: 29343545.
    Citations: 1     Fields:    Translation:HumansAnimalsCells
  29. Kochert BA, Iacob RE, Wales TE, Makriyannis A, Engen JR. Hydrogen-Deuterium Exchange Mass Spectrometry to Study Protein Complexes. Methods Mol Biol. 2018; 1764:153-171. PMID: 29605914.
    Citations:    
  30. Moroco JA, Alvarado JJ, Staudt RP, Shi H, Wales TE, Smithgall TE, Engen JR. Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding. J Mol Biol. 2018 02 02; 430(3):310-321. PMID: 29258818.
    Citations:    
  31. Lamberto I, Liu X, Seo HS, Schauer NJ, Iacob RE, Hu W, Das D, Mikhailova T, Weisberg EL, Engen JR, Anderson KC, Chauhan D, Dhe-Paganon S, Buhrlage SJ. Structure-Guided Development of a Potent and Selective Non-covalent Active-Site Inhibitor of USP7. Cell Chem Biol. 2017 12 21; 24(12):1490-1500.e11. PMID: 29056421.
    Citations: 6     Fields:    Translation:HumansCells
  32. Kerres N, Steurer S, Schlager S, Bader G, Berger H, Caligiuri M, Dank C, Engen JR, Ettmayer P, Fischerauer B, Flotzinger G, Gerlach D, Gerstberger T, Gmaschitz T, Greb P, Han B, Heyes E, Iacob RE, Kessler D, Kölle H, Lamarre L, Lancia DR, Lucas S, Mayer M, Mayr K, Mischerikow N, Mück K, Peinsipp C, Petermann O, Reiser U, Rudolph D, Rumpel K, Salomon C, Scharn D, Schnitzer R, Schrenk A, Schweifer N, Thompson D, Traxler E, Varecka R, Voss T, Weiss-Puxbaum A, Winkler S, Zheng X, Zoephel A, Kraut N, McConnell D, Pearson M, Koegl M. Chemically Induced Degradation of the Oncogenic Transcription Factor BCL6. Cell Rep. 2017 Sep 19; 20(12):2860-2875. PMID: 28930682.
    Citations:    
  33. Joseph RE, Wales TE, Fulton DB, Engen JR, Andreotti AH. Achieving a Graded Immune Response: BTK Adopts a Range of Active/Inactive Conformations Dictated by Multiple Interdomain Contacts. Structure. 2017 10 03; 25(10):1481-1494.e4. PMID: 28867612.
    Citations:    
  34. Bhat JY, Milicic G, Thieulin-Pardo G, Bracher A, Maxwell A, Ciniawsky S, Mueller-Cajar O, Engen JR, Hartl FU, Wendler P, Hayer-Hartl M. Mechanism of Enzyme Repair by the AAA+ Chaperone Rubisco Activase. Mol Cell. 2017 Sep 07; 67(5):744-756.e6. PMID: 28803776.
    Citations: 3     Fields:    Translation:Cells
  35. Lu J, Harrison RA, Li L, Zeng M, Gondi S, Scott D, Gray NS, Engen JR, Westover KD. KRAS G12C Drug Development: Discrimination between Switch II Pocket Configurations Using Hydrogen/Deuterium-Exchange Mass Spectrometry. Structure. 2017 09 05; 25(9):1442-1448.e3. PMID: 28781083.
    Citations: 1     Fields:    Translation:HumansCells
  36. Pritz JR, Wachter F, Lee S, Luccarelli J, Wales TE, Cohen DT, Coote P, Heffron GJ, Engen JR, Massefski W, Walensky LD. Allosteric sensitization of proapoptotic BAX. Nat Chem Biol. 2017 Sep; 13(9):961-967. PMID: 28692068.
    Citations: 5     Fields:    Translation:HumansCells
  37. Chen TF, Sazinsky SL, Houde D, DiLillo DJ, Bird J, Li KK, Cheng GT, Qiu H, Engen JR, Ravetch JV, Wittrup KD. Engineering Aglycosylated IgG Variants with Wild-Type or Improved Binding Affinity to Human Fc Gamma RIIA and Fc Gamma RIIIAs. J Mol Biol. 2017 08 04; 429(16):2528-2541. PMID: 28694069.
    Citations:    
  38. Chaikuad A, Filippakopoulos P, Marcsisin SR, Picaud S, Schröder M, Sekine S, Ichijo H, Engen JR, Takeda K, Knapp S. Structures of PGAM5 Provide Insight into Active Site Plasticity and Multimeric Assembly. Structure. 2017 07 05; 25(7):1089-1099.e3. PMID: 28648608.
    Citations:    
  39. Wales TE, Fadgen KE, Eggertson MJ, Engen JR. Subzero Celsius separations in three-zone temperature controlled hydrogen deuterium exchange mass spectrometry. J Chromatogr A. 2017 Nov 10; 1523:275-282. PMID: 28596009.
    Citations:    
  40. Dong X, Zhao B, Iacob RE, Zhu J, Koksal AC, Lu C, Engen JR, Springer TA. Force interacts with macromolecular structure in activation of TGF-ß. Nature. 2017 02 02; 542(7639):55-59. PMID: 28117447.
    Citations: 19     Fields:    Translation:HumansCells
  41. Das M, Wilson CJ, Mei X, Wales T, Engen JR, Gursky O. Structural stability and local dynamics in disease-causing mutants of human apolipoprotein a-I: what makes the protein amyloidogenic? Amyloid. 2017 Mar; 24(sup1):11-12. PMID: 28042708.
    Citations:    
  42. Harrison RA, Lu J, Carrasco M, Hunter J, Manandhar A, Gondi S, Westover KD, Engen JR. Structural Dynamics in Ras and Related Proteins upon Nucleotide Switching. J Mol Biol. 2016 11 20; 428(23):4723-4735. PMID: 27751724.
    Citations:    
  43. Harrison RA, Engen JR. Conformational insight into multi-protein signaling assemblies by hydrogen-deuterium exchange mass spectrometry. Curr Opin Struct Biol. 2016 12; 41:187-193. PMID: 27552080.
    Citations:    
  44. Huang RY, Iacob RE, Krystek SR, Jin M, Wei H, Tao L, Das TK, Tymiak AA, Engen JR, Chen G. Characterization of Aggregation Propensity of a Human Fc-Fusion Protein Therapeutic by Hydrogen/Deuterium Exchange Mass Spectrometry. J Am Soc Mass Spectrom. 2017 05; 28(5):795-802. PMID: 27527097.
    Citations:    
  45. Badger J, Grover P, Shi H, Panjarian SB, Engen JR, Smithgall TE, Makowski L. c-Abl Tyrosine Kinase Adopts Multiple Active Conformational States in Solution. Biochemistry. 2016 06 14; 55(23):3251-60. PMID: 27166638.
    Citations:    
  46. Lee S, Wales TE, Escudero S, Cohen DT, Luccarelli J, Gallagher CG, Cohen NA, Huhn AJ, Bird GH, Engen JR, Walensky LD. Allosteric inhibition of antiapoptotic MCL-1. Nat Struct Mol Biol. 2016 06; 23(6):600-7. PMID: 27159560.
    Citations: 3     Fields:    Translation:HumansAnimalsCells
  47. Wales TE, Poe JA, Emert-Sedlak L, Morgan CR, Smithgall TE, Engen JR. Hydrogen Exchange Mass Spectrometry of Related Proteins with Divergent Sequences: A Comparative Study of HIV-1 Nef Allelic Variants. J Am Soc Mass Spectrom. 2016 06; 27(6):1048-61. PMID: 27032648.
    Citations:    
  48. Chopra N, Wales TE, Joseph RE, Boyken SE, Engen JR, Jernigan RL, Andreotti AH. Dynamic Allostery Mediated by a Conserved Tryptophan in the Tec Family Kinases. PLoS Comput Biol. 2016 Mar; 12(3):e1004826. PMID: 27010561.
    Citations:    
  49. Roberts JM, Tarafdar S, Joseph RE, Andreotti AH, Smithgall TE, Engen JR, Wales TE. Dynamics of the Tec-family tyrosine kinase SH3 domains. Protein Sci. 2016 Apr; 25(4):852-64. PMID: 26808198.
    Citations:    
  50. Shi Y, Chen X, Elsasser S, Stocks BB, Tian G, Lee BH, Shi Y, Zhang N, de Poot SA, Tuebing F, Sun S, Vannoy J, Tarasov SG, Engen JR, Finley D, Walters KJ. Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome. Science. 2016 Feb 19; 351(6275). PMID: 26912900.
    Citations: 31     Fields:    Translation:AnimalsCells
  51. Guttman M, Wales TE, Whittington D, Engen JR, Brown JM, Lee KK. Tuning a High Transmission Ion Guide to Prevent Gas-Phase Proton Exchange During H/D Exchange MS Analysis. J Am Soc Mass Spectrom. 2016 Apr; 27(4):662-8. PMID: 26810432.
    Citations:    
  52. Das M, Wilson CJ, Mei X, Wales TE, Engen JR, Gursky O. Structural Stability and Local Dynamics in Disease-Causing Mutants of Human Apolipoprotein A-I: What Makes the Protein Amyloidogenic? J Mol Biol. 2016 Jan 29; 428(2 Pt B):449-62. PMID: 26562506.
    Citations:    
  53. Wales TE, Hochrein JM, Morgan CR, Emert-Sedlak LA, Smithgall TE, Engen JR. Subtle Dynamic Changes Accompany Hck Activation by HIV-1 Nef and are Reversed by an Antiretroviral Kinase Inhibitor. Biochemistry. 2015 Oct 20; 54(41):6382-91. PMID: 26440750.
    Citations:    
  54. Pirrone GF, Emert-Sedlak LA, Wales TE, Smithgall TE, Kent MS, Engen JR. Membrane-Associated Conformation of HIV-1 Nef Investigated with Hydrogen Exchange Mass Spectrometry at a Langmuir Monolayer. Anal Chem. 2015 Jul 21; 87(14):7030-5. PMID: 26133569.
    Citations:    
  55. Pirrone GF, Vernon BC, Kent MS, Engen JR. Hydrogen Exchange Mass Spectrometry of Proteins at Langmuir Monolayers. Anal Chem. 2015 Jul 21; 87(14):7022-9. PMID: 26134943.
    Citations:    
  56. Black WA, Stocks BB, Mellors JS, Engen JR, Ramsey JM. Utilizing Microchip Capillary Electrophoresis Electrospray Ionization for Hydrogen Exchange Mass Spectrometry. Anal Chem. 2015 Jun 16; 87(12):6280-7. PMID: 25992468.
    Citations:    
  57. Marcsisin SR, Liptak C, Marineau J, Bradner JE, Engen JR. Tag and Capture Flow Hydrogen Exchange Mass Spectrometry with a Fluorous-Immobilized Probe. Anal Chem. 2015 Jun 16; 87(12):6349-56. PMID: 26023704.
    Citations:    Fields:    
  58. Engen JR, Wales TE. Analytical Aspects of Hydrogen Exchange Mass Spectrometry. Annu Rev Anal Chem (Palo Alto Calif). 2015; 8:127-48. PMID: 26048552.
    Citations:    
  59. Nevin P, Lu X, Zhang K, Engen JR, Beuning PJ. Noncognate DNA damage prevents the formation of the active conformation of the Y-family DNA polymerases DinB and DNA polymerase ?. FEBS J. 2015 Jul; 282(14):2646-60. PMID: 25899385.
    Citations:    
  60. Iacob RE, Krystek SR, Huang RY, Wei H, Tao L, Lin Z, Morin PE, Doyle ML, Tymiak AA, Engen JR, Chen G. Hydrogen/deuterium exchange mass spectrometry applied to IL-23 interaction characteristics: potential impact for therapeutics. Expert Rev Proteomics. 2015 Apr; 12(2):159-69. PMID: 25711416.
    Citations:    
  61. Barclay LA, Wales TE, Garner TP, Wachter F, Lee S, Guerra RM, Stewart ML, Braun CR, Bird GH, Gavathiotis E, Engen JR, Walensky LD. Inhibition of Pro-apoptotic BAX by a noncanonical interaction mechanism. Mol Cell. 2015 Mar 05; 57(5):873-886. PMID: 25684204.
    Citations: 24     Fields:    Translation:HumansCells
  62. Nevin P, Engen JR, Beuning PJ. Steric gate residues of Y-family DNA polymerases DinB and pol kappa are crucial for dNTP-induced conformational change. DNA Repair (Amst). 2015 May; 29:65-73. PMID: 25684709.
    Citations:    
  63. Moroco JA, Engen JR. Replication in bioanalytical studies with HDX MS: aim as high as possible. Bioanalysis. 2015; 7(9):1065-7. PMID: 26039804.
    Citations:    
  64. Pirrone GF, Iacob RE, Engen JR. Applications of hydrogen/deuterium exchange MS from 2012 to 2014. Anal Chem. 2015 Jan 06; 87(1):99-118. PMID: 25398026.
    Citations:    
  65. Iacob RE, Chen G, Ahn J, Houel S, Wei H, Mo J, Tao L, Cohen D, Xie D, Lin Z, Morin PE, Doyle ML, Tymiak AA, Engen JR. The influence of adnectin binding on the extracellular domain of epidermal growth factor receptor. J Am Soc Mass Spectrom. 2014 Dec; 25(12):2093-102. PMID: 25223306.
    Citations:    
  66. Boyken SE, Chopra N, Xie Q, Joseph RE, Wales TE, Fulton DB, Engen JR, Jernigan RL, Andreotti AH. A conserved isoleucine maintains the inactive state of Bruton's tyrosine kinase. J Mol Biol. 2014 Oct 23; 426(21):3656-69. PMID: 25193673.
    Citations:    
  67. Moroco JA, Craigo JK, Iacob RE, Wales TE, Engen JR, Smithgall TE. Differential sensitivity of Src-family kinases to activation by SH3 domain displacement. PLoS One. 2014; 9(8):e105629. PMID: 25144189.
    Citations:    
  68. Wang W, Iacob RE, Luoh RP, Engen JR, Lippard SJ. Electron transfer control in soluble methane monooxygenase. J Am Chem Soc. 2014 Jul 09; 136(27):9754-62. PMID: 24937475.
    Citations:    
  69. Georgescauld F, Popova K, Gupta AJ, Bracher A, Engen JR, Hayer-Hartl M, Hartl FU. GroEL/ES chaperonin modulates the mechanism and accelerates the rate of TIM-barrel domain folding. Cell. 2014 May 08; 157(4):922-934. PMID: 24813614.
    Citations:    
  70. Fang J, Nevin P, Kairys V, Venclovas C, Engen JR, Beuning PJ. Conformational analysis of processivity clamps in solution demonstrates that tertiary structure does not correlate with protein dynamics. Structure. 2014 Apr 08; 22(4):572-581. PMID: 24613485.
    Citations:    
  71. Parker CH, Morgan CR, Rand KD, Engen JR, Jorgenson JW, Stafford DW. A conformational investigation of propeptide binding to the integral membrane protein ?-glutamyl carboxylase using nanodisc hydrogen exchange mass spectrometry. Biochemistry. 2014 Mar 11; 53(9):1511-20. PMID: 24512177.
    Citations:    
  72. Lim SM, Westover KD, Ficarro SB, Harrison RA, Choi HG, Pacold ME, Carrasco M, Hunter J, Kim ND, Xie T, Sim T, Jänne PA, Meyerson M, Marto JA, Engen JR, Gray NS. Therapeutic targeting of oncogenic K-Ras by a covalent catalytic site inhibitor. Angew Chem Int Ed Engl. 2014 Jan 03; 53(1):199-204. PMID: 24259466.
    Citations: 65     Fields:    Translation:Cells
  73. Shi XE, Wales TE, Elkin C, Kawahata N, Engen JR, Annis DA. Hydrogen exchange-mass spectrometry measures stapled peptide conformational dynamics and predicts pharmacokinetic properties. Anal Chem. 2013 Dec 03; 85(23):11185-8. PMID: 24215480.
    Citations:    
  74. Iacob RE, Bou-Assaf GM, Makowski L, Engen JR, Berkowitz SA, Houde D. Investigating monoclonal antibody aggregation using a combination of H/DX-MS and other biophysical measurements. J Pharm Sci. 2013 Dec; 102(12):4315-29. PMID: 24136070.
    Citations:    
  75. Akgun B, Satija S, Nanda H, Pirrone GF, Shi X, Engen JR, Kent MS. Conformational transition of membrane-associated terminally acylated HIV-1 Nef. Structure. 2013 Oct 08; 21(10):1822-33. PMID: 24035710.
    Citations:    
  76. Guttman M, Weis DD, Engen JR, Lee KK. Analysis of overlapped and noisy hydrogen/deuterium exchange mass spectra. J Am Soc Mass Spectrom. 2013 Dec; 24(12):1906-12. PMID: 24018862.
    Citations:    
  77. Joseph RE, Kleino I, Wales TE, Xie Q, Fulton DB, Engen JR, Berg LJ, Andreotti AH. Activation loop dynamics determine the different catalytic efficiencies of B cell- and T cell-specific tec kinases. Sci Signal. 2013 Aug 27; 6(290):ra76. PMID: 23982207.
    Citations:    
  78. Wei H, Mo J, Tao L, Russell RJ, Tymiak AA, Chen G, Iacob RE, Engen JR. Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications. Drug Discov Today. 2014 Jan; 19(1):95-102. PMID: 23928097.
    Citations:    
  79. Karageorgos I, Wales TE, Janero DR, Zvonok N, Vemuri VK, Engen JR, Makriyannis A. Active-site inhibitors modulate the dynamic properties of human monoacylglycerol lipase: a hydrogen exchange mass spectrometry study. Biochemistry. 2013 Jul 23; 52(29):5016-26. PMID: 23795559.
    Citations: 4     Fields:    Translation:HumansCells
  80. Tiyanont K, Wales TE, Siebel CW, Engen JR, Blacklow SC. Insights into Notch3 activation and inhibition mediated by antibodies directed against its negative regulatory region. J Mol Biol. 2013 Sep 09; 425(17):3192-204. PMID: 23747483.
    Citations: 5     Fields:    Translation:HumansCells
  81. Tsukamoto K, Wales TE, Daniels K, Pal R, Sheng R, Cho W, Stafford W, Engen JR, Krieger M, Kocher O. Noncanonical role of the PDZ4 domain of the adaptor protein PDZK1 in the regulation of the hepatic high density lipoprotein receptor scavenger receptor class B, type I (SR-BI). J Biol Chem. 2013 Jul 05; 288(27):19845-60. PMID: 23720744.
    Citations: 5     Fields:    Translation:HumansAnimalsCells
  82. Nasr ML, Shi X, Bowman AL, Johnson M, Zvonok N, Janero DR, Vemuri VK, Wales TE, Engen JR, Makriyannis A. Membrane phospholipid bilayer as a determinant of monoacylglycerol lipase kinetic profile and conformational repertoire. Protein Sci. 2013 Jun; 22(6):774-87. PMID: 23553709.
    Citations: 13     Fields:    Translation:HumansCells
  83. Panjarian S, Iacob RE, Chen S, Engen JR, Smithgall TE. Structure and dynamic regulation of Abl kinases. J Biol Chem. 2013 Feb 22; 288(8):5443-50. PMID: 23316053.
    Citations:    
  84. Panjarian S, Iacob RE, Chen S, Wales TE, Engen JR, Smithgall TE. Enhanced SH3/linker interaction overcomes Abl kinase activation by gatekeeper and myristic acid binding pocket mutations and increases sensitivity to small molecule inhibitors. J Biol Chem. 2013 Mar 01; 288(9):6116-29. PMID: 23303187.
    Citations:    
  85. Houde D, Engen JR. Conformational analysis of recombinant monoclonal antibodies with hydrogen/deuterium exchange mass spectrometry. Methods Mol Biol. 2013; 988:269-89. PMID: 23475726.
    Citations:    
  86. Engen JR, Wales TE, Chen S, Marzluff EM, Hassell KM, Weis DD, Smithgall TE. Partial cooperative unfolding in proteins as observed by hydrogen exchange mass spectrometry. Int Rev Phys Chem. 2013 Jan 01; 32(1):96-127. PMID: 23682200.
    Citations:    
  87. Wales TE, Eggertson MJ, Engen JR. Considerations in the analysis of hydrogen exchange mass spectrometry data. Methods Mol Biol. 2013; 1007:263-88. PMID: 23666730.
    Citations:    
  88. Alapafuja SO, Nikas SP, Bharathan IT, Shukla VG, Nasr ML, Bowman AL, Zvonok N, Li J, Shi X, Engen JR, Makriyannis A. Sulfonyl fluoride inhibitors of fatty acid amide hydrolase. J Med Chem. 2012 Nov 26; 55(22):10074-89. PMID: 23083016.
    Citations: 5     Fields:    Translation:HumansAnimalsCells
  89. Ahn J, Cao MJ, Yu YQ, Engen JR. Accessing the reproducibility and specificity of pepsin and other aspartic proteases. Biochim Biophys Acta. 2013 Jun; 1834(6):1222-9. PMID: 23063535.
    Citations:    
  90. Ahn J, Jung MC, Wyndham K, Yu YQ, Engen JR. Pepsin immobilized on high-strength hybrid particles for continuous flow online digestion at 10,000 psi. Anal Chem. 2012 Aug 21; 84(16):7256-62. PMID: 22856522.
    Citations:    
  91. Sörensen PM, Iacob RE, Fritzsche M, Engen JR, Brieher WM, Charras G, Eggert US. The natural product cucurbitacin E inhibits depolymerization of actin filaments. ACS Chem Biol. 2012 Sep 21; 7(9):1502-8. PMID: 22724897.
    Citations: 21     Fields:    Translation:HumansAnimalsCells
  92. Berkowitz SA, Engen JR, Mazzeo JR, Jones GB. Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars. Nat Rev Drug Discov. 2012 Jun 29; 11(7):527-40. PMID: 22743980.
    Citations:    
  93. Iacob RE, Engen JR. Hydrogen exchange mass spectrometry: are we out of the quicksand? J Am Soc Mass Spectrom. 2012 Jun; 23(6):1003-10. PMID: 22476891.
    Citations:    
  94. Karageorgos I, Zvonok N, Janero DR, Vemuri VK, Shukla V, Wales TE, Engen JR, Makriyannis A. Endocannabinoid enzyme engineering: soluble human thio-monoacylglycerol lipase (sol-S-hMGL). ACS Chem Neurosci. 2012 May 16; 3(5):393-9. PMID: 22860208.
    Citations: 4     Fields:    Translation:Humans
  95. Pene-Dumitrescu T, Shu ST, Wales TE, Alvarado JJ, Shi H, Narute P, Moroco JA, Yeh JI, Engen JR, Smithgall TE. HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck. BMC Chem Biol. 2012 Mar 15; 12:1. PMID: 22420777.
    Citations:    
  96. Choi SH, Wales TE, Nam Y, O'Donovan DJ, Sliz P, Engen JR, Blacklow SC. Conformational locking upon cooperative assembly of notch transcription complexes. Structure. 2012 Feb 08; 20(2):340-9. PMID: 22325781.
    Citations: 23     Fields:    Translation:HumansCells
  97. Wei H, Ahn J, Yu YQ, Tymiak A, Engen JR, Chen G. Using hydrogen/deuterium exchange mass spectrometry to study conformational changes in granulocyte colony stimulating factor upon PEGylation. J Am Soc Mass Spectrom. 2012 Mar; 23(3):498-504. PMID: 22227798.
    Citations:    
  98. Yu W, Chory EJ, Wernimont AK, Tempel W, Scopton A, Federation A, Marineau JJ, Qi J, Barsyte-Lovejoy D, Yi J, Marcellus R, Iacob RE, Engen JR, Griffin C, Aman A, Wienholds E, Li F, Pineda J, Estiu G, Shatseva T, Hajian T, Al-Awar R, Dick JE, Vedadi M, Brown PJ, Arrowsmith CH, Bradner JE, Schapira M. Catalytic site remodelling of the DOT1L methyltransferase by selective inhibitors. Nat Commun. 2012; 3:1288. PMID: 23250418.
    Citations: 77     Fields:    Translation:HumansCells
  99. Kim M, Sun ZY, Rand KD, Shi X, Song L, Cheng Y, Fahmy AF, Majumdar S, Ofek G, Yang Y, Kwong PD, Wang JH, Engen JR, Wagner G, Reinherz EL. Antibody mechanics on a membrane-bound HIV segment essential for GP41-targeted viral neutralization. Nat Struct Mol Biol. 2011 Oct 16; 18(11):1235-43. PMID: 22002224.
    Citations: 42     Fields:    Translation:HumansCells
  100. Marcsisin SR, Narute PS, Emert-Sedlak LA, Kloczewiak M, Smithgall TE, Engen JR. On the solution conformation and dynamics of the HIV-1 viral infectivity factor. J Mol Biol. 2011 Jul 29; 410(5):1008-22. PMID: 21763503.
    Citations:    
  101. Rand KD, Pringle SD, Morris M, Engen JR, Brown JM. ETD in a traveling wave ion guide at tuned Z-spray ion source conditions allows for site-specific hydrogen/deuterium exchange measurements. J Am Soc Mass Spectrom. 2011 Oct; 22(10):1784-93. PMID: 21952892.
    Citations:    
  102. Morgan CR, Hebling CM, Rand KD, Stafford DW, Jorgenson JW, Engen JR. Conformational transitions in the membrane scaffold protein of phospholipid bilayer nanodiscs. Mol Cell Proteomics. 2011 Sep; 10(9):M111.010876. PMID: 21715319.
    Citations:    
  103. Fang J, Engen JR, Beuning PJ. Escherichia coli processivity clamp ß from DNA polymerase III is dynamic in solution. Biochemistry. 2011 Jul 05; 50(26):5958-68. PMID: 21657794.
    Citations:    
  104. Fang J, Rand KD, Beuning PJ, Engen JR. False EX1 signatures caused by sample carryover during HX MS analyses. Int J Mass Spectrom. 2011 Apr 30; 302(1-3):19-25. PMID: 21643454.
    Citations:    
  105. Tiyanont K, Wales TE, Aste-Amezaga M, Aster JC, Engen JR, Blacklow SC. Evidence for increased exposure of the Notch1 metalloprotease cleavage site upon conversion to an activated conformation. Structure. 2011 Apr 13; 19(4):546-54. PMID: 21481777.
    Citations: 23     Fields:    Translation:HumansCells
  106. Morgan CR, Miglionico BV, Engen JR. Effects of HIV-1 Nef on human N-myristoyltransferase 1. Biochemistry. 2011 Apr 26; 50(16):3394-403. PMID: 21449607.
    Citations:    
  107. Iacob RE, Zhang J, Gray NS, Engen JR. Allosteric interactions between the myristate- and ATP-site of the Abl kinase. PLoS One. 2011 Jan 10; 6(1):e15929. PMID: 21264348.
    Citations: 25     Fields:    Translation:HumansCells
  108. Houde D, Berkowitz SA, Engen JR. The utility of hydrogen/deuterium exchange mass spectrometry in biopharmaceutical comparability studies. J Pharm Sci. 2011 Jun; 100(6):2071-86. PMID: 21491437.
    Citations:    
  109. Kent MS, Murton JK, Sasaki DY, Satija S, Akgun B, Nanda H, Curtis JE, Majewski J, Morgan CR, Engen JR. Neutron reflectometry study of the conformation of HIV Nef bound to lipid membranes. Biophys J. 2010 Sep 22; 99(6):1940-8. PMID: 20858440.
    Citations:    
  110. Marcsisin SR, Engen JR. Molecular insight into the conformational dynamics of the Elongin BC complex and its interaction with HIV-1 Vif. J Mol Biol. 2010 Oct 08; 402(5):892-904. PMID: 20728451.
    Citations:    
  111. Hebling CM, Morgan CR, Stafford DW, Jorgenson JW, Rand KD, Engen JR. Conformational analysis of membrane proteins in phospholipid bilayer nanodiscs by hydrogen exchange mass spectrometry. Anal Chem. 2010 Jul 01; 82(13):5415-9. PMID: 20518534.
    Citations:    
  112. Fang J, Rand KD, Silva MC, Wales TE, Engen JR, Beuning PJ. Conformational dynamics of the Escherichia coli DNA polymerase manager proteins UmuD and UmuD'. J Mol Biol. 2010 Apr 23; 398(1):40-53. PMID: 20206636.
    Citations:    
  113. Marcsisin SR, Engen JR. Hydrogen exchange mass spectrometry: what is it and what can it tell us? Anal Bioanal Chem. 2010 Jun; 397(3):967-72. PMID: 20195578.
    Citations:    
  114. Houde D, Peng Y, Berkowitz SA, Engen JR. Post-translational modifications differentially affect IgG1 conformation and receptor binding. Mol Cell Proteomics. 2010 Aug; 9(8):1716-28. PMID: 20103567.
    Citations:    
  115. Zhang J, Adrián FJ, Jahnke W, Cowan-Jacob SW, Li AG, Iacob RE, Sim T, Powers J, Dierks C, Sun F, Guo GR, Ding Q, Okram B, Choi Y, Wojciechowski A, Deng X, Liu G, Fendrich G, Strauss A, Vajpai N, Grzesiek S, Tuntland T, Liu Y, Bursulaya B, Azam M, Manley PW, Engen JR, Daley GQ, Warmuth M, Gray NS. Targeting Bcr-Abl by combining allosteric with ATP-binding-site inhibitors. Nature. 2010 Jan 28; 463(7280):501-6. PMID: 20072125.
    Citations: 157     Fields:    Translation:HumansAnimalsCells
  116. Zhou W, Ercan D, Chen L, Yun CH, Li D, Capelletti M, Cortot AB, Chirieac L, Iacob RE, Padera R, Engen JR, Wong KK, Eck MJ, Gray NS, Jänne PA. Novel mutant-selective EGFR kinase inhibitors against EGFR T790M. Nature. 2009 Dec 24; 462(7276):1070-4. PMID: 20033049.
    Citations: 247     Fields:    Translation:AnimalsCells
  117. Rand KD, Pringle SD, Murphy JP, Fadgen KE, Brown J, Engen JR. Gas-phase hydrogen/deuterium exchange in a traveling wave ion guide for the examination of protein conformations. Anal Chem. 2009 Dec 15; 81(24):10019-28. PMID: 19921790.
    Citations:    
  118. Morgan CR, Engen JR. Investigating solution-phase protein structure and dynamics by hydrogen exchange mass spectrometry. Curr Protoc Protein Sci. 2009 Nov; Chapter 17:Unit 17.6.1-17. PMID: 19937720.
    Citations:    
  119. Engen JR. Analysis of protein conformation and dynamics by hydrogen/deuterium exchange MS. Anal Chem. 2009 Oct 01; 81(19):7870-5. PMID: 19788312.
    Citations:    
  120. Houde D, Arndt J, Domeier W, Berkowitz S, Engen JR. Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry. Anal Chem. 2009 Jul 15; 81(14):5966. PMID: 19606834.
    Citations:    
  121. Houde D, Arndt J, Domeier W, Berkowitz S, Engen JR. Characterization of IgG1 conformation and conformational dynamics by hydrogen/deuterium exchange mass spectrometry. Anal Chem. 2009 Apr 01; 81(7):2644-51. PMID: 19265386.
    Citations:    
  122. De Luca V, Maria G, De Mauro G, Catara G, Carginale V, Ruggiero G, Capasso A, Parisi E, Brier S, Engen JR, Capasso C. Aspartic proteinases in Antarctic fish. Mar Genomics. 2009 Mar; 2(1):1-10. PMID: 21798166.
    Citations:    
  123. Iacob RE, Pene-Dumitrescu T, Zhang J, Gray NS, Smithgall TE, Engen JR. Conformational disturbance in Abl kinase upon mutation and deregulation. Proc Natl Acad Sci U S A. 2009 Feb 03; 106(5):1386-91. PMID: 19164531.
    Citations: 35     Fields:    Translation:AnimalsCells
  124. Engen JR, Wales TE, Hochrein JM, Meyn MA, Banu Ozkan S, Bahar I, Smithgall TE. Structure and dynamic regulation of Src-family kinases. Cell Mol Life Sci. 2008 Oct; 65(19):3058-73. PMID: 18563293.
    Citations:    
  125. Iacob RE, Murphy JP, Engen JR. Ion mobility adds an additional dimension to mass spectrometric analysis of solution-phase hydrogen/deuterium exchange. Rapid Commun Mass Spectrom. 2008 Sep; 22(18):2898-904. PMID: 18727141.
    Citations:    
  126. Chen S, O'Reilly LP, Smithgall TE, Engen JR. Tyrosine phosphorylation in the SH3 domain disrupts negative regulatory interactions within the c-Abl kinase core. J Mol Biol. 2008 Nov 07; 383(2):414-23. PMID: 18775435.
    Citations:    
  127. Wales TE, Fadgen KE, Gerhardt GC, Engen JR. High-speed and high-resolution UPLC separation at zero degrees Celsius. Anal Chem. 2008 Sep 01; 80(17):6815-20. PMID: 18672890.
    Citations:    
  128. Chen S, Dumitrescu TP, Smithgall TE, Engen JR. Abl N-terminal cap stabilization of SH3 domain dynamics. Biochemistry. 2008 May 27; 47(21):5795-803. PMID: 18452309.
    Citations:    
  129. Brier S, Maria G, Carginale V, Capasso A, Wu Y, Taylor RM, Borotto NB, Capasso C, Engen JR. Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii. FEBS J. 2007 Dec; 274(23):6152-66. PMID: 17976195.
    Citations:    
  130. Trible RP, Emert-Sedlak L, Wales TE, Ayyavoo V, Engen JR, Smithgall TE. Allosteric loss-of-function mutations in HIV-1 Nef from a long-term non-progressor. J Mol Biol. 2007 Nov 16; 374(1):121-9. PMID: 17920628.
    Citations:    
  131. Chen S, Brier S, Smithgall TE, Engen JR. The Abl SH2-kinase linker naturally adopts a conformation competent for SH3 domain binding. Protein Sci. 2007 Apr; 16(4):572-81. PMID: 17327393.
    Citations:    
  132. Mitchell JL, Trible RP, Emert-Sedlak LA, Weis DD, Lerner EC, Applen JJ, Sefton BM, Smithgall TE, Engen JR. Functional characterization and conformational analysis of the Herpesvirus saimiri Tip-C484 protein. J Mol Biol. 2007 Mar 02; 366(4):1282-93. PMID: 17207813.
    Citations:    
  133. Weis DD, Kjellen P, Sefton BM, Engen JR. Altered dynamics in Lck SH3 upon binding to the LBD1 domain of Herpesvirus saimiri Tip. Protein Sci. 2006 Oct; 15(10):2402-10. PMID: 17008721.
    Citations:    
  134. Weis DD, Engen JR, Kass IJ. Semi-automated data processing of hydrogen exchange mass spectra using HX-Express. J Am Soc Mass Spectrom. 2006 Dec; 17(12):1700-3. PMID: 16931036.
    Citations:    
  135. Meyn MA, Wilson MB, Abdi FA, Fahey N, Schiavone AP, Wu J, Hochrein JM, Engen JR, Smithgall TE. Src family kinases phosphorylate the Bcr-Abl SH3-SH2 region and modulate Bcr-Abl transforming activity. J Biol Chem. 2006 Oct 13; 281(41):30907-16. PMID: 16912036.
    Citations:    
  136. Weis DD, Wales TE, Engen JR, Hotchko M, Ten Eyck LF. Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis. J Am Soc Mass Spectrom. 2006 Nov; 17(11):1498-1509. PMID: 16875839.
    Citations:    
  137. Hochrein JM, Wales TE, Lerner EC, Schiavone AP, Smithgall TE, Engen JR. Conformational features of the full-length HIV and SIV Nef proteins determined by mass spectrometry. Biochemistry. 2006 Jun 27; 45(25):7733-9. PMID: 16784224.
    Citations:    
  138. Wu Y, Kaveti S, Engen JR. Extensive deuterium back-exchange in certain immobilized pepsin columns used for H/D exchange mass spectrometry. Anal Chem. 2006 Mar 01; 78(5):1719-23. PMID: 16503628.
    Citations:    
  139. Wales TE, Engen JR. Partial unfolding of diverse SH3 domains on a wide timescale. J Mol Biol. 2006 Apr 14; 357(5):1592-604. PMID: 16487539.
    Citations:    
  140. Wu Y, Engen JR, Hobbins WB. Ultra performance liquid chromatography (UPLC) further improves hydrogen/deuterium exchange mass spectrometry. J Am Soc Mass Spectrom. 2006 Feb; 17(2):163-7. PMID: 16406808.
    Citations:    
  141. Wales TE, Engen JR. Hydrogen exchange mass spectrometry for the analysis of protein dynamics. Mass Spectrom Rev. 2006 Jan-Feb; 25(1):158-70. PMID: 16208684.
    Citations:    
  142. Kaveti S, Engen JR. Protein interactions probed with mass spectrometry. Methods Mol Biol. 2006; 316:179-97. PMID: 16671405.
    Citations:    
  143. Hochrein JM, Lerner EC, Schiavone AP, Smithgall TE, Engen JR. An examination of dynamics crosstalk between SH2 and SH3 domains by hydrogen/deuterium exchange and mass spectrometry. Protein Sci. 2006 Jan; 15(1):65-73. PMID: 16322569.
    Citations:    
  144. Lu X, Li L, Feng X, Wu Y, Dunaway-Mariano D, Engen JR, Mariano PS. L-canavanine is a time-controlled mechanism-based inhibitor of Pseudomonas aeruginosa arginine deiminase. J Am Chem Soc. 2005 Nov 30; 127(47):16412-3. PMID: 16305225.
    Citations:    
  145. Lerner EC, Trible RP, Schiavone AP, Hochrein JM, Engen JR, Smithgall TE. Activation of the Src family kinase Hck without SH3-linker release. J Biol Chem. 2005 Dec 09; 280(49):40832-7. PMID: 16210316.
    Citations:    
  146. Carter JM, Gurevich VV, Prossnitz ER, Engen JR. Conformational differences between arrestin2 and pre-activated mutants as revealed by hydrogen exchange mass spectrometry. J Mol Biol. 2005 Aug 26; 351(4):865-78. PMID: 16045931.
    Citations:    
  147. Caldeira JC, Wu Y, Mameli M, Purdy RH, Li PK, Akwa Y, Savage DD, Engen JR, Valenzuela CF. Fetal alcohol exposure alters neurosteroid levels in the developing rat brain. J Neurochem. 2004 Sep; 90(6):1530-9. PMID: 15341536.
    Citations:    
  148. Wu Y, Engen JR. What mass spectrometry can reveal about protein function. Analyst. 2004 Apr; 129(4):290-6. PMID: 15042158.
    Citations:    
  149. Heil DP, Engen J, Higginson BK. Influence of ski pole grip on peak upper body power output in cross-country skiers. Eur J Appl Physiol. 2004 Apr; 91(4):481-7. PMID: 14872248.
    Citations:    
  150. Engen JR. Analysis of protein complexes with hydrogen exchange and mass spectrometry. Analyst. 2003 Jun; 128(6):623-8. PMID: 12866878.
    Citations:    
  151. Takashima Y, Delfino FJ, Engen JR, Superti-Furga G, Smithgall TE. Regulation of c-Fes tyrosine kinase activity by coiled-coil and SH2 domains: analysis with Saccharomyces cerevisiae. Biochemistry. 2003 Apr 01; 42(12):3567-74. PMID: 12653561.
    Citations:    
  152. Harris MN, Burchiel SW, Winyard PG, Engen JR, Mobarak CD, Timmins GS. Determining the site of spin trapping of the equine myoglobin radical by combined use of EPR, electrophoretic purification, and mass spectrometry. Chem Res Toxicol. 2002 Dec; 15(12):1589-94. PMID: 12482241.
    Citations:    
  153. Engen JR, Bradbury EM, Chen X. Using stable-isotope-labeled proteins for hydrogen exchange studies in complex mixtures. Anal Chem. 2002 Apr 01; 74(7):1680-6. PMID: 12033260.
    Citations:    
  154. Dorey K, Engen JR, Kretzschmar J, Wilm M, Neubauer G, Schindler T, Superti-Furga G. Phosphorylation and structure-based functional studies reveal a positive and a negative role for the activation loop of the c-Abl tyrosine kinase. Oncogene. 2001 Dec 06; 20(56):8075-84. PMID: 11781820.
    Citations:    
  155. Engen JR, Smith DL. Investigating protein structure and dynamics by hydrogen exchange MS. Anal Chem. 2001 May 01; 73(9):256A-265A. PMID: 11354508.
    Citations:    
  156. Gmeiner WH, Xu I, Horita DA, Smithgall TE, Engen JR, Smith DL, Byrd RA. Intramolecular binding of a proximal PPII helix to an SH3 domain in the fusion protein SH3Hck : PPIIhGAP. Cell Biochem Biophys. 2001; 35(2):115-26. PMID: 11892787.
    Citations:    
  157. Engen JR, Smith DL. Investigating the higher order structure of proteins. Hydrogen exchange, proteolytic fragmentation, and mass spectrometry. Methods Mol Biol. 2000; 146:95-112. PMID: 10948498.
    Citations:    
  158. Engen JR, Gmeiner WH, Smithgall TE, Smith DL. Hydrogen exchange shows peptide binding stabilizes motions in Hck SH2. Biochemistry. 1999 Jul 13; 38(28):8926-35. PMID: 10413466.
    Citations:    
  159. Engen JR, Smithgall TE, Gmeiner WH, Smith DL. Comparison of SH3 and SH2 domain dynamics when expressed alone or in an SH(3+2) construct: the role of protein dynamics in functional regulation. J Mol Biol. 1999 Apr 02; 287(3):645-56. PMID: 10092465.
    Citations:    
  160. Engen JR, Smithgall TE, Gmeiner WH, Smith DL. Identification and localization of slow, natural, cooperative unfolding in the hematopoietic cell kinase SH3 domain by amide hydrogen exchange and mass spectrometry. Biochemistry. 1997 Nov 25; 36(47):14384-91. PMID: 9398156.
    Citations:    
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.