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Douglas Glenn Brownfield, Ph.D.

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Overview
The Brownfield lab aims to reverse-engineer developmental processes for the rational synthesis of tissues and organs. The long-term goal of the lab is to understand how tissues are constructed during development, maintained in adulthood, and disrupted in disease. To achieve this goal, the lab will harness single-cell transcriptomics, microfabricated 3D organotypic culture, and genetically-engineered mouse models. Focusing on the lung, Dr. Brownfield's previous work mapping the cellular and molecular programs that drive alveolar epithelial differentiation in the mouse lung have resulted in reconstruction of the full gene expression program as well as identification of the long-sought signal that selects and maintains throughout life alveolar epithelial cell fate. The lab hopes to translate its findings to applications for lung diseases such as bronchopulmonary dysplasia, pulmonary fibrosis, chronic obstructive pulmonary disease, and emphysema.

Dr. Douglas Brownfield was appointed the Mark and Catherine Winkler Assistant Professor of Cell and Developmental Biology in the Department of Environmental Health in July of 2019. He received a BS in Biomedical Engineering from Tulane University and a PhD in Bioengineering from the University of California, Berkeley (mentored by Dr. Mina Bissell) with a minor in Management of Technology from the Haas School of Business. He completed a postdoctoral fellowship in the Department of Biochemistry at Stanford University (mentored by Dr. Mark Krasnow). Dr. Brownfield has received the NIH Pathway to Independence Award, American Cancer Society Postdoctoral Fellowship, and the NSF Graduate Research fellowship.

Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R00HL127267 (BROWNFIELD, DOUGLAS GLENN) Aug 15, 2019 - Jul 31, 2022
    NIH
    Reverse Engineering the Alveolus: From cellular to microenvironment specification during development
    Role: Principal Investigator
  2. K99HL127267 (BROWNFIELD, DOUGLAS GLENN) Feb 15, 2016 - Jan 31, 2019
    NIH
    Reverse Engineering the Alveolus: From cellular to microenvironment specification during development
    Role: Principal Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Inde Z, Yapp C, Joshi GN, Spetz J, Fraser C, Deskin B, Ghelfi E, Sodhi C, Hackam D, Kobzik L, Croker B, Brownfield D, Jia H, Sarosiek KA. Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity. bioRxiv. 2020 Sep 13. PMID: 32935109.
    Citations:    
  2. Ricca BL, Venugopalan G, Furuta S, Tanner K, Orellana WA, Reber CD, Brownfield DG, Bissell MJ, Fletcher DA. Transient external force induces phenotypic reversion of malignant epithelial structures via nitric oxide signaling. Elife. 2018 03 21; 7. PMID: 29560858.
    Citations: 10     Fields:    Translation:HumansCells
  3. Nabhan AN, Brownfield DG, Harbury PB, Krasnow MA, Desai TJ. Single-cell Wnt signaling niches maintain stemness of alveolar type 2 cells. Science. 2018 03 09; 359(6380):1118-1123. PMID: 29420258.
    Citations: 132     Fields:    Translation:AnimalsCells
  4. Nam S, Lee J, Brownfield DG, Chaudhuri O. Viscoplasticity Enables Mechanical Remodeling of Matrix by Cells. Biophys J. 2016 Nov 15; 111(10):2296-2308. PMID: 27851951.
    Citations: 31     Fields:    Translation:AnimalsCells
  5. Vaughan AE, Brumwell AN, Xi Y, Gotts JE, Brownfield DG, Treutlein B, Tan K, Tan V, Liu FC, Looney MR, Matthay MA, Rock JR, Chapman HA. Lineage-negative progenitors mobilize to regenerate lung epithelium after major injury. Nature. 2015 Jan 29; 517(7536):621-5. PMID: 25533958.
    Citations: 207     Fields:    Translation:HumansAnimalsCells
  6. Treutlein B, Brownfield DG, Wu AR, Neff NF, Mantalas GL, Espinoza FH, Desai TJ, Krasnow MA, Quake SR. Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq. Nature. 2014 May 15; 509(7500):371-5. PMID: 24739965.
    Citations: 478     Fields:    Translation:AnimalsCells
  7. Desai TJ, Brownfield DG, Krasnow MA. Alveolar progenitor and stem cells in lung development, renewal and cancer. Nature. 2014 Mar 13; 507(7491):190-4. PMID: 24499815.
    Citations: 293     Fields:    Translation:AnimalsCells
  8. Brownfield DG, Venugopalan G, Lo A, Mori H, Tanner K, Fletcher DA, Bissell MJ. Patterned collagen fibers orient branching mammary epithelium through distinct signaling modules. Curr Biol. 2013 Apr 22; 23(8):703-9. PMID: 23562267.
    Citations: 51     Fields:    Translation:AnimalsCells
  9. Alcaraz J, Mori H, Ghajar CM, Brownfield D, Galgoczy R, Bissell MJ. Collective epithelial cell invasion overcomes mechanical barriers of collagenous extracellular matrix by a narrow tube-like geometry and MMP14-dependent local softening. Integr Biol (Camb). 2011 Dec; 3(12):1153-66. PMID: 21993836.
    Citations: 21     Fields:    Translation:HumansCells
  10. Chanson L, Brownfield D, Garbe JC, Kuhn I, Stampfer MR, Bissell MJ, LaBarge MA. Self-organization is a dynamic and lineage-intrinsic property of mammary epithelial cells. Proc Natl Acad Sci U S A. 2011 Feb 22; 108(8):3264-9. PMID: 21300877.
    Citations: 30     Fields:    Translation:HumansCells
  11. Alcaraz J, Xu R, Mori H, Nelson CM, Mroue R, Spencer VA, Brownfield D, Radisky DC, Bustamante C, Bissell MJ. Laminin and biomimetic extracellular elasticity enhance functional differentiation in mammary epithelia. EMBO J. 2008 Nov 05; 27(21):2829-38. PMID: 18843297.
    Citations: 78     Fields:    Translation:AnimalsCells
  12. Martin K, Brownfield D, Karmonik C, Sanford L, Torres L, Insull W, Morrisett J. Short-term tracking of atherosclerosis in operated and unoperated human carotid arteries by high resolution magnetic resonance imaging. World J Surg. 2007 Apr; 31(4):723-32. PMID: 17354027.
    Citations:    Fields:    Translation:HumansCTClinical Trials
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.