Harvard Catalyst Profiles

Contact, publication, and social network information about Harvard faculty and fellows.

David Walt, Ph.D.

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Institution
Department
Address
Other Positions
Title
Institution
Department

Mentoring
Available: 01/02/21, Expires: 10/31/23

Our overall goal is to develop a simple blood test that, when combined with mammographic imaging, can detect aggressive breast cancers early and accurately. Our aims are twofold: 1) Reduce the false-positive rates of mammographic screening. Mammographic screening for breast cancer is limited due to its high false-positive rate, resulting in unnecessary biopsies, high costs, and high patient morbidity. Mammography can detect a visible mass or calcifications but cannot distinguish between cancer and non-cancer in all cases. A blood biomarker test integrated with mammographic screening would provide additional biological information and disease stratification that could reduce the number of screen-detected false positives. 2) Accurately detect aggressive cancers that require treatment early. The high false-negative rate of current screening methods misses cancers that require treatment. Furthermore, our inability to differentiate aggressive from indolent cancer is a significant clinical problem that leads to overdiagnosis and overtreatment. A blood biomarker test integrated with mammographic imaging could provide additional biological information and disease stratification to improve early and accurate detection of aggressive cancers and reduce overdiagnosis and overtreatment. The student will identify biomarker candidates, learn the process of developing an ultrasensitive immunoassay, and test for the biomarkers in clinical blood samples as we examine the ability of the panel to discriminate subtypes of breast cancer and in samples preceding clinical diagnosis.

Available: 11/01/21, Expires: 10/31/23

This project focuses on translating the ultrasensitive digital ELISA platforms developed in the Walt lab to an integrated point-of-care device for blood-based detection of tuberculosis. We have previously developed a digital ELISA strategy with attomolar limits of protein detection, based on the generation of a localized fluorescent signal on single immunocomplexes captured on antibody-coated beads. This interdisciplinary project will combine work in microfluidics, analytical chemistry, and clinical diagnostics. Students will contribute to development and optimization of the point-of-care device, including sample processing and signal readout modules. Clinical validation of point-of-care assays for measuring both HIV and tuberculosis biomarker panels will be performed. Basic wet lab skills are required, and students with experiences in various areas such as microfabrication, programming, or infectious disease diagnostics are welcomed. The project will be carried out at the Wyss Institute for Biologically Inspired Engineering and Brigham and Women's Hospital (Hale Building for Transformative Medicine).

Available: 11/01/21, Expires: 10/31/23

We are developing new highly sensitive diagnostics for the detection of neurodegenerative disease (Alzheimer's, Parkinson's) from blood. The project is focused on defining and isolating brain-derived biomarkers in plasma, such as neuron-specific extracellular vesicles (and their RNA/protein contents), cell-free DNA, and proteins. We are using a variety of experimental and computational techniques to isolate and analyze these biomarkers, including high throughput sequencing and Single Molecule Array (Simoa) immunoassays. The project involves several aspects of technology development towards the isolation and measurement of these biomarkers, as well as testing them in patient biofluid samples. Working with a team of neuroscientists and bioengineers, a medical student would help to optimize protocols for both cell type specific EV isolation from Neurons and Glia as well as to explore total EV isolation in plasma and Cerebrospinal fluid. The medical student can pursue new molecular targets they are interested in while also contributing to the team's overall methods development goals.


Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R01HD097081 (MARON, JILL LAMANNA) Aug 9, 2019 - Apr 30, 2024
    NIH
    Salivary Diagnostics for Sepsis Screening in the Neonate
    Role: Co-Principal Investigator
  2. U01NS097051 (WALT, DAVID R.) Jul 15, 2016 - Nov 30, 2019
    NIH
    Detection of Post-Translationally Modified Proteins as a Biomarker Panel for Parkinson's Disease
    Role: Principal Investigator
  3. R25OD010547 (WALT, DAVID R.) Jun 1, 2012 - Jun 30, 2017
    NIH
    Bioinformatics Inquiry through Sequencing (BIOSEQ)
    Role: Principal Investigator
  4. R01HG006021 (WALT, DAVID R.) Nov 18, 2010 - Jul 31, 2015
    NIH
    Development of new tools for Single Cell Analysis
    Role: Principal Investigator
  5. U01DE017788 (WALT, DAVID R.) Sep 1, 2006 - Feb 28, 2013
    NIH
    Microsensor Arrays for Saliva Diagnostics
    Role: Principal Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Swank Z, Senussi Y, Manickas-Hill Z, Yu XG, Li JZ, Alter G, Walt DR. Reply to Alaedini and Wormser. Clin Infect Dis. 2022 Nov 17. PMID: 36385394.
    Citations:    Fields:    
  2. Swank Z, Senussi Y, Manickas-Hill Z, Yu XG, Li JZ, Alter G, Walt DR. Persistent circulating SARS-CoV-2 spike is associated with post-acute COVID-19 sequelae. Clin Infect Dis. 2022 Sep 02. PMID: 36052466.
    Citations: 7     Fields:    
  3. Sherman AC, Desjardins M, Cheng CA, Bausk B, Izaguirre N, Zhou G, Krauss J, Tolan N, Walt DR, Soiffer R, Ho VT, Issa NC, Baden LR. Severe Acute Respiratory Syndrome Coronavirus 2 Messenger RNA Vaccines in Allogeneic Hematopoietic Stem Cell Transplant Recipients: Immunogenicity and Reactogenicity. Clin Infect Dis. 2022 Aug 24; 75(1):e920-e923. PMID: 34726754; PMCID: PMC8689898.
    Citations: 4     Fields:    
  4. Sherman AC, Crombie JL, Cheng C, Desjardins M, Zhou G, Ometoruwa O, Rooks R, Senussi Y, McDonough M, Guerrero LI, Kupelian J, Doss-Gollin S, Smolen KK, van Haren SD, Armand P, Levy O, Walt DR, Baden LR, Issa NC. Immunogenicity of a Three-Dose Primary Series of mRNA COVID-19 Vaccines in Patients With Lymphoid Malignancies. Open Forum Infect Dis. 2022 Aug; 9(8):ofac417. PMID: 36043177.
    Citations:    
  5. Kawser Z, Hossain M, Suliman S, Lockman S, Gitaka J, Bandawe G, Rahmat R, Hasan I, Siddik AB, Afrad MH, Rahman MZ, Miller G, Walt DR, Ivers LC, LaRocque RC, Harris JB, Qadri F. An Assessment of a Rapid SARS-CoV-2 Antigen Test in Bangladesh. Am J Trop Med Hyg. 2022 10 12; 107(4):845-849. PMID: 35970285; PMCID: PMC9651516.
    Citations:    Fields:    Translation:HumansCells
  6. Najjar D, Rainbow J, Sharma Timilsina S, Jolly P, de Puig H, Yafia M, Durr N, Sallum H, Alter G, Li JZ, Yu XG, Walt DR, Paradiso JA, Estrela P, Collins JJ, Ingber DE. A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma. Nat Biomed Eng. 2022 08; 6(8):968-978. PMID: 35941191; PMCID: PMC9361916.
    Citations: 3     Fields:    Translation:HumansCells
  7. Cabrera C, Pilobello K, Dalvin S, Bobrow J, Shah D, Garg LF, Chalise S, Doyle P, Miller GA, Walt DR, Suliman S, Jolly P. Systematic Approach to Address Early Pandemic's Diagnostic Unmet Needs. Front Microbiol. 2022; 13:910156. PMID: 35783392; PMCID: PMC9247567.
    Citations:    
  8. Rajurkar M, Parikh AR, Solovyov A, You E, Kulkarni AS, Chu C, Xu KH, Jaicks C, Taylor MS, Wu C, Alexander KA, Good CR, Szabolcs A, Gerstberger S, Tran AV, Xu N, Ebright RY, Van Seventer EE, Vo KD, Tai EC, Lu C, Joseph-Chazan J, Raabe MJ, Nieman LT, Desai N, Arora KS, Ligorio M, Thapar V, Cohen L, Garden PM, Senussi Y, Zheng H, Allen JN, Blaszkowsky LS, Clark JW, Goyal L, Wo JY, Ryan DP, Corcoran RB, Deshpande V, Rivera MN, Aryee MJ, Hong TS, Berger SL, Walt DR, Burns KH, Park PJ, Greenbaum BD, Ting DT. Reverse Transcriptase Inhibition Disrupts Repeat Element Life Cycle in Colorectal Cancer. Cancer Discov. 2022 06 02; 12(6):1462-1481. PMID: 35320348; PMCID: PMC9167735.
    Citations:    Fields:    Translation:HumansAnimals
  9. Burnum-Johnson KE, Conrads TP, Drake RR, Herr AE, Iyengar R, Kelly RT, Lundberg E, MacCoss MJ, Naba A, Nolan GP, Pevzner PA, Rodland KD, Sechi S, Slavov N, Spraggins JM, Van Eyk JE, Vidal M, Vogel C, Walt DR, Kelleher NL. New Views of Old Proteins: Clarifying the Enigmatic Proteome. Mol Cell Proteomics. 2022 07; 21(7):100254. PMID: 35654359; PMCID: PMC9256833.
    Citations:    Fields:    
  10. Windsor WJ, Roell Y, Tucker H, Cheng CA, Suliman S, Peek LJ, Pestano GA, Lee WT, Zeichhardt H, Lamb MM, Kammel M, Wang H, Kedl R, Rester C, Morrison TE, Davenport BJ, Carson K, Yates J, Howard K, Kulas K, Walt DR, Dafni A, Taylor D, Chu M. Harmonization of Multiple SARS-CoV-2 Reference Materials Using the WHO IS (NIBSC 20/136): Results and Implications. Front Microbiol. 2022; 13:893801. PMID: 35707164; PMCID: PMC9190986.
    Citations:    
  11. Gilboa T, Ogata AF, Reilly CB, Walt DR. Single-molecule studies reveal method for tuning the heterogeneous activity of alkaline phosphatase. Biophys J. 2022 06 07; 121(11):2027-2034. PMID: 35527401; PMCID: PMC9247479.
    Citations:    Fields:    Translation:AnimalsCells
  12. Goyal G, Prabhala P, Mahajan G, Bausk B, Gilboa T, Xie L, Zhai Y, Lazarovits R, Mansour A, Kim MS, Patil A, Curran D, Long JM, Sharma S, Junaid A, Cohen L, Ferrante TC, Levy O, Prantil-Baun R, Walt DR, Ingber DE. Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ-on-a-Chip. Adv Sci (Weinh). 2022 05; 9(14):e2103241. PMID: 35289122; PMCID: PMC9109055.
    Citations: 3     Fields:    Translation:HumansAnimalsPHPublic Health
  13. Norman M, Gilboa T, Walt DR. High-Sensitivity Single Molecule Array Assays for Pathological Isoforms in Parkinson's Disease. Clin Chem. 2022 03 04; 68(3):431-440. PMID: 35064661.
    Citations:    Fields:    Translation:Humans
  14. Ogata AF, Cheng CA, Desjardins M, Senussi Y, Sherman AC, Powell M, Novack L, Von S, Li X, Baden LR, Walt DR. Circulating Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Vaccine Antigen Detected in the Plasma of mRNA-1273 Vaccine Recipients. Clin Infect Dis. 2022 03 01; 74(4):715-718. PMID: 34015087; PMCID: PMC8241425.
    Citations: 28     Fields:    Translation:HumansCells
  15. Yonker LM, Swank Z, Gilboa T, Senussi Y, Kenyon V, Papadakis L, Boribong BP, Carroll RW, Walt DR, Fasano A. Zonulin Antagonist, Larazotide (AT1001), As an Adjuvant Treatment for Multisystem Inflammatory Syndrome in Children: A Case Series. Crit Care Explor. 2022 Feb; 10(2):e0641. PMID: 35211683; PMCID: PMC8860335.
    Citations: 1     
  16. Wu C, Dougan TJ, Walt DR. High-Throughput, High-Multiplex Digital Protein Detection with Attomolar Sensitivity. ACS Nano. 2022 01 25; 16(1):1025-1035. PMID: 35029381; PMCID: PMC9499451.
    Citations: 1     Fields:    
  17. Gibson CJ, Kim HT, Zhao L, Murdock HM, Hambley B, Ogata A, Madero-Marroquin R, Wang S, Green L, Fleharty M, Dougan T, Cheng CA, Blumenstiel B, Cibulskis C, Tsuji J, Duran M, Gocke CD, Antin JH, Nikiforow S, DeZern AE, Chen YB, Ho VT, Jones RJ, Lennon NJ, Walt DR, Ritz J, Soiffer RJ, Gondek LP, Lindsley RC. Donor Clonal Hematopoiesis and Recipient Outcomes After Transplantation. J Clin Oncol. 2022 01 10; 40(2):189-201. PMID: 34793200; PMCID: PMC8718176.
    Citations: 6     Fields:    Translation:Humans
  18. Ter-Ovanesyan D, Norman M, Lazarovits R, Trieu W, Lee JH, Church GM, Walt DR. Framework for rapid comparison of extracellular vesicle isolation methods. Elife. 2021 11 16; 10. PMID: 34783650.
    Citations: 4     Fields:    Translation:HumansCells
  19. Gilboa T, Cohen L, Cheng CA, Lazarovits R, Uwamanzu-Nna A, Han I, Griswold K, Barry N, Thompson DB, Kohman RE, Woolley AE, Karlson EW, Walt DR. A SARS-CoV-2 Neutralization Assay Using Single Molecule Arrays. Angew Chem Int Ed Engl. 2021 12 01; 60(49):25966-25972. PMID: 34534408.
    Citations: 2     Fields:    Translation:HumansCells
  20. Ogata AF, Lazarovits R, Uwamanzu-Nna A, Gilboa T, Cheng CA, Walt DR. Severe Acute Respiratory Syndrome Coronavirus 2 Antigens as Targets of Antibody Responses. Clin Lab Med. 2022 03; 42(1):97-109. PMID: 35153051; PMCID: PMC8563368.
    Citations:    Fields:    Translation:HumansCells
  21. Nilles EJ, Karlson EW, Norman M, Gilboa T, Fischinger S, Atyeo C, Zhou G, Bennett CL, Tolan NV, Oganezova K, Walt DR, Alter G, Simmons DP, Schur P, Jarolim P, Woolley AE, Baden LR. Evaluation of Three Commercial and Two Non-Commercial Immunoassays for the Detection of Prior Infection to SARS-CoV-2. J Appl Lab Med. 2021 11 01; 6(6):1561-1570. PMID: 34196711; PMCID: PMC8420636.
    Citations: 3     Fields:    Translation:HumansCells
  22. Langellotto F, Dellacherie MO, Yeager C, Ijaz H, Yu J, Cheng CA, Dimitrakakis N, Seiler BT, Gebre MS, Gilboa T, Johnson R, Storm N, Bardales S, Graveline A, White D, Tringides CM, Cartwright MJ, Doherty EJ, Honko A, Griffiths A, Barouch DH, Walt DR, Mooney DJ. A Modular Biomaterial Scaffold-Based Vaccine Elicits Durable Adaptive Immunity to Subunit SARS-CoV-2 Antigens. Adv Healthc Mater. 2021 11; 10(22):e2101370. PMID: 34605223.
    Citations: 3     Fields:    Translation:HumansCells
  23. Gilboa T, Ogata AF, Walt DR. Single-Molecule Enzymology for Diagnostics: Profiling Alkaline Phosphatase Activity in Clinical Samples. Chembiochem. 2022 01 05; 23(1):e202100358. PMID: 34375495.
    Citations:    Fields:    Translation:Humans
  24. Crombie JL, Sherman AC, Cheng CA, Ryan CE, Zon R, Desjardins M, Baker P, McDonough M, Izaguirre N, Bausk B, Krauss J, Gilboa T, Senussi Y, Walt DR, Davids MS, Brown JR, Armand P, Baden LR, Issa N. Activity of mRNA COVID-19 vaccines in patients with lymphoid malignancies. Blood Adv. 2021 08 24; 5(16):3062-3065. PMID: 34387646; PMCID: PMC8362656.
    Citations: 4     Fields:    Translation:HumansCells
  25. Mysore V, Cullere X, Settles ML, Ji X, Kattan MW, Desjardins M, Durbin-Johnson B, Gilboa T, Baden LR, Walt DR, Lichtman AH, Jehi L, Mayadas TN. Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens. Med (N Y). 2021 09 10; 2(9):1050-1071.e7. PMID: 34414383; PMCID: PMC8363466.
    Citations: 12     Translation:HumansCells
  26. Yonker LM, Gilboa T, Ogata AF, Senussi Y, Lazarovits R, Boribong BP, Bartsch YC, Loiselle M, Rivas MN, Porritt RA, Lima R, Davis JP, Farkas EJ, Burns MD, Young N, Mahajan VS, Hajizadeh S, Lopez XIH, Kreuzer J, Morris R, Martinez EE, Han I, Griswold K, Barry NC, Thompson DB, Church G, Edlow AG, Haas W, Pillai S, Arditi M, Alter G, Walt DR, Fasano A. Multisystem inflammatory syndrome in children is driven by zonulin-dependent loss of gut mucosal barrier. J Clin Invest. 2021 07 15; 131(14). PMID: 34032635; PMCID: PMC8279585.
    Citations: 42     Fields:    Translation:HumansCells
  27. Trombetta BA, Kandigian SE, Kitchen RR, Grauwet K, Webb PK, Miller GA, Jennings CG, Jain S, Miller S, Kuo Y, Sweeney T, Gilboa T, Norman M, Simmons DP, Ramirez CE, Bedard M, Fink C, Ko J, De León Peralta EJ, Watts G, Gomez-Rivas E, Davis V, Barilla RM, Wang J, Cunin P, Bates S, Morrison-Smith C, Nicholson B, Wong E, El-Mufti L, Kann M, Bolling A, Fortin B, Ventresca H, Zhou W, Pardo S, Kwock M, Hazra A, Cheng L, Ahmad QR, Toombs JA, Larson R, Pleskow H, Luo NM, Samaha C, Pandya UM, De Silva P, Zhou S, Ganhadeiro Z, Yohannes S, Gay R, Slavik J, Mukerji SS, Jarolim P, Walt DR, Carlyle BC, Ritterhouse LL, Suliman S. Correction to: Evaluation of serological lateral flow assays for severe acute respiratory syndrome coronavirus-2. BMC Infect Dis. 2021 Jul 01; 21(1):628. PMID: 34210278.
    Citations:    Fields:    
  28. Polinski JM, Zimin AV, Clark KF, Kohn AB, Sadowski N, Timp W, Ptitsyn A, Khanna P, Romanova DY, Williams P, Greenwood SJ, Moroz LL, Walt DR, Bodnar AG. The American lobster genome reveals insights on longevity, neural, and immune adaptations. Sci Adv. 2021 06; 7(26). PMID: 34162536; PMCID: PMC8221624.
    Citations: 5     Fields:    Translation:Animals
  29. Trombetta BA, Kandigian SE, Kitchen RR, Grauwet K, Webb PK, Miller GA, Jennings CG, Jain S, Miller S, Kuo Y, Sweeney T, Gilboa T, Norman M, Simmons DP, Ramirez CE, Bedard M, Fink C, Ko J, De León Peralta EJ, Watts G, Gomez-Rivas E, Davis V, Barilla RM, Wang J, Cunin P, Bates S, Morrison-Smith C, Nicholson B, Wong E, El-Mufti L, Kann M, Bolling A, Fortin B, Ventresca H, Zhou W, Pardo S, Kwock M, Hazra A, Cheng L, Ahmad QR, Toombs JA, Larson R, Pleskow H, Luo NM, Samaha C, Pandya UM, De Silva P, Zhou S, Ganhadeiro Z, Yohannes S, Gay R, Slavik J, Mukerji SS, Jarolim P, Walt DR, Carlyle BC, Ritterhouse LL, Suliman S. Evaluation of serological lateral flow assays for severe acute respiratory syndrome coronavirus-2. BMC Infect Dis. 2021 Jun 16; 21(1):580. PMID: 34134647.
    Citations: 5     Fields:    Translation:HumansCells
  30. Norman M, Ter-Ovanesyan D, Trieu W, Lazarovits R, Kowal EJK, Lee JH, Chen-Plotkin AS, Regev A, Church GM, Walt DR. L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma. Nat Methods. 2021 06; 18(6):631-634. PMID: 34092791; PMCID: PMC9075416.
    Citations: 18     Fields:    Translation:HumansCells
  31. Mysore V, Cullere X, Settles ML, Ji X, Kattan MW, Desjardins M, Durbin-Johnson B, Gilboa T, Baden LR, Walt DR, Lichtman A, Jehi L, Mayadas TN. Protective heterologous T cell immunity in COVID-19 induced by MMR and Tdap vaccine antigens. bioRxiv. 2021 May 04. PMID: 33972940.
    Citations:    
  32. Ter-Ovanesyan D, Gilboa T, Lazarovits R, Rosenthal A, Yu X, Li JZ, Church GM, Walt DR. Ultrasensitive Measurement of Both SARS-CoV-2 RNA and Antibodies from Saliva. Anal Chem. 2021 04 06; 93(13):5365-5370. PMID: 33755419.
    Citations: 6     Fields:    Translation:HumansCells
  33. Etson CM, Todorov P, Shatery Nejad N, Shrestha N, Walt DR. Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage. J Vis Exp. 2021 02 07; (168). PMID: 33616095.
    Citations:    Fields:    Translation:Cells
  34. Ogata AF, Maley AM, Wu C, Gilboa T, Norman M, Lazarovits R, Mao CP, Newton G, Chang M, Nguyen K, Kamkaew M, Zhu Q, Gibson TE, Ryan ET, Charles RC, Marasco WA, Walt DR. Ultra-Sensitive Serial Profiling of SARS-CoV-2 Antigens and Antibodies in Plasma to Understand Disease Progression in COVID-19 Patients with Severe Disease. Clin Chem. 2020 12 01; 66(12):1562-1572. PMID: 32897389.
    Citations: 42     Fields:    Translation:HumansCells
  35. Ben-Othman R, Cai B, Liu AC, Varankovich N, He D, Blimkie TM, Lee AH, Gill EE, Novotny M, Aevermann B, Drissler S, Shannon CP, McCann S, Marty K, Bjornson G, Edgar RD, Lin DTS, Gladish N, Maclsaac J, Amenyogbe N, Chan Q, Llibre A, Collin J, Landais E, Le K, Reiss SM, Koff WC, Havenar-Daughton C, Heran M, Sangha B, Walt D, Krajden M, Crotty S, Sok D, Briney B, Burton DR, Duffy D, Foster LJ, Mohn WW, Kobor MS, Tebbutt SJ, Brinkman RR, Scheuermann RH, Hancock REW, Kollmann TR, Sadarangani M. Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults. Front Immunol. 2020; 11:580373. PMID: 33250895.
    Citations: 6     Fields:    Translation:HumansCellsPHPublic HealthCTClinical Trials
  36. Maley AM, Garden PM, Walt DR. Simplified Digital Enzyme-Linked Immunosorbent Assay Using Tyramide Signal Amplification and Fibrin Hydrogels. ACS Sens. 2020 10 23; 5(10):3037-3042. PMID: 32988208.
    Citations: 3     Fields:    
  37. Keegan A, Ricciuti B, Garden P, Cohen L, Nishihara R, Adeni A, Paweletz C, Supplee J, Jänne PA, Severgnini M, Awad MM, Walt DR. Plasma IL-6 changes correlate to PD-1 inhibitor responses in NSCLC. J Immunother Cancer. 2020 10; 8(2). PMID: 33020238.
    Citations: 20     Fields:    Translation:Humans
  38. Norman M, Gilboa T, Ogata AF, Maley AM, Cohen L, Busch EL, Lazarovits R, Mao CP, Cai Y, Zhang J, Feldman JE, Hauser BM, Caradonna TM, Chen B, Schmidt AG, Alter G, Charles RC, Ryan ET, Walt DR. Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19. Nat Biomed Eng. 2020 12; 4(12):1180-1187. PMID: 32948854; PMCID: PMC7498988.
    Citations: 50     Fields:    Translation:HumansCellsPHPublic Health
  39. Gilboa T, Maley AM, Ogata AF, Wu C, Walt DR. Sequential Protein Capture in Multiplex Single Molecule Arrays: A Strategy for Eliminating Assay Cross-Reactivity. Adv Healthc Mater. 2021 02; 10(4):e2001111. PMID: 32893488; PMCID: PMC8238389.
    Citations: 3     Fields:    Translation:HumansCells
  40. Haug V, Kollar B, Tasigiorgos S, Endo Y, Kauke M, Safi AF, Veeramani A, Abdulrazzak O, Bausk B, Walt D, Pomahac B. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours. Transplantation. 2020 09; 104(9):e260-e270. PMID: 32168040.
    Citations: 1     Fields:    Translation:Humans
  41. Wang X, Ogata AF, Walt DR. Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays. J Am Chem Soc. 2020 09 02; 142(35):15098-15106. PMID: 32797755.
    Citations:    Fields:    Translation:HumansCells
  42. Jiang Y, Li X, Walt DR. Single-Molecule Analysis Determines Isozymes of Human Alkaline Phosphatase in Serum. Angew Chem Int Ed Engl. 2020 10 05; 59(41):18010-18015. PMID: 32613710.
    Citations: 4     Fields:    Translation:HumansCells
  43. Adans-Dester CP, Bamberg S, Bertacchi FP, Caulfield B, Chappie K, Demarchi D, Erb MK, Estrada J, Fabara EE, Freni M, Friedl KE, Ghaffari R, Gill G, Greenberg MS, Hoyt RW, Jovanov E, Kanzler CM, Katabi D, Kernan M, Kigin C, Lee SI, Leonhardt S, Lovell NH, Mantilla J, McCoy TH, Luo NM, Miller GA, Moore J, O'Keeffe D, Palmer J, Parisi F, Patel S, Po J, Pugliese BL, Quatieri T, Rahman T, Ramasarma N, Rogers JA, Ruiz-Esparza GU, Sapienza S, Schiurring G, Schwamm L, Shafiee H, Kelly Silacci S, Sims NM, Talkar T, Tharion WJ, Toombs JA, Uschnig C, Vergara-Diaz GP, Wacnik P, Wang MD, Welch J, Williamson L, Zafonte R, Zai A, Zhang YT, Tearney GJ, Ahmad R, Walt DR, Bonato P. Can mHealth Technology Help Mitigate the Effects of the COVID-19 Pandemic? IEEE Open J Eng Med Biol. 2020; 1:243-248. PMID: 34192282; PMCID: PMC8023427.
    Citations: 18     
  44. Wang X, Walt DR. Simultaneous detection of small molecules, proteins and microRNAs using single molecule arrays. Chem Sci. 2020 Jul 08; 11(30):7896-7903. PMID: 34094160.
    Citations: 3     
  45. Cohen L, Cui N, Cai Y, Garden PM, Li X, Weitz DA, Walt DR. Single Molecule Protein Detection with Attomolar Sensitivity Using Droplet Digital Enzyme-Linked Immunosorbent Assay. ACS Nano. 2020 08 25; 14(8):9491-9501. PMID: 32589401.
    Citations: 15     Fields:    
  46. Wu C, Garden PM, Walt DR. Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays. J Am Chem Soc. 2020 07 15; 142(28):12314-12323. PMID: 32602703.
    Citations: 13     Fields:    Translation:Humans
  47. Nilles EJ, Karlson EW, Norman M, Gilboa T, Fischinger S, Atyeo C, Zhou G, Bennett CL, Tolan NV, Oganezova K, Walt DR, Alter G, Simmons DP, Schur P, Jarolim P, Baden LR. Evaluation of two commercial and two non-commercial immunoassays for the detection of prior infection to SARS-CoV-2. medRxiv. 2020 Jun 26. PMID: 32607518; PMCID: PMC7325183.
    Citations:    
  48. Norman M, Gilboa T, Ogata AF, Maley AM, Cohen L, Cai Y, Zhang J, Feldman JE, Hauser BM, Caradonna TM, Chen B, Schmidt AG, Alter G, Charles RC, Ryan ET, Walt DR. Ultra-Sensitive High-Resolution Profiling of Anti-SARS-CoV-2 Antibodies for Detecting Early Seroconversion in COVID-19 Patients. medRxiv. 2020 May 02. PMID: 32511657.
    Citations:    
  49. Cohen L, Keegan A, Walt DR. Single-Molecule Arrays for Ultrasensitive Detection of Blood-Based Biomarkers for Immunotherapy. Methods Mol Biol. 2020; 2055:399-412. PMID: 31502162.
    Citations: 1     Fields:    Translation:Humans
  50. Wu C, Maley AM, Walt DR. Single-molecule measurements in microwells for clinical applications. Crit Rev Clin Lab Sci. 2019 Dec 22; 1-21. PMID: 31865834.
    Citations: 3     Fields:    
  51. Ahmad R, Xie L, Pyle M, Suarez MF, Broger T, Steinberg D, Ame SM, Lucero MG, Szucs MJ, MacMullan M, Berven FS, Dutta A, Sanvictores DM, Tallo VL, Bencher R, Eisinger DP, Dhingra U, Deb S, Ali SM, Mehta S, Fawzi WW, Riley ID, Sazawal S, Premji Z, Black R, Murray CJL, Rodriguez B, Carr SA, Walt DR, Gillette MA. A rapid triage test for active pulmonary tuberculosis in adult patients with persistent cough. Sci Transl Med. 2019 10 23; 11(515). PMID: 31645455.
    Citations: 16     Fields:    Translation:Humans
  52. Jiang Y, Li X, Morrow BR, Pothukuchy A, Gollihar J, Novak R, Reilly CB, Ellington AD, Walt DR. Single-Molecule Mechanistic Study of Enzyme Hysteresis. ACS Cent Sci. 2019 Oct 23; 5(10):1691-1698. PMID: 31660437; PMCID: PMC6813718.
    Citations: 2     
  53. Walt DR. Clinical testing should be individualized, not based on populations. J Clin Invest. 2019 09 03; 129(9):3472-3473. PMID: 31478913; PMCID: PMC6715350.
    Citations: 1     Fields:    Translation:Humans
  54. Duffy DC, Walt DR. Protein Detection by Counting Molecules. Clin Chem. 2019 06; 65(6):809-810. PMID: 30692115.
    Citations: 2     Fields:    Translation:Humans
  55. Cohen L, Keegan A, Melanson SEF, Walt DR. Impact of clinical sample handling and processing on ultra-low level measurements of plasma cytokines. Clin Biochem. 2019 Mar; 65:38-44. PMID: 30633878.
    Citations: 8     Fields:    Translation:Humans
  56. Wang X, Cohen L, Wang J, Walt DR. Competitive Immunoassays for the Detection of Small Molecules Using Single Molecule Arrays. J Am Chem Soc. 2018 12 26; 140(51):18132-18139. PMID: 30495929.
    Citations: 24     Fields:    Translation:HumansAnimals
  57. Cohen L, Walt DR. Evaluation of Antibody Biotinylation Approaches for Enhanced Sensitivity of Single Molecule Array (Simoa) Immunoassays. Bioconjug Chem. 2018 10 17; 29(10):3452-3458. PMID: 30272951.
    Citations: 5     Fields:    
  58. Hwang SS, Chan H, Sorci M, Van Deventer J, Wittrup D, Belfort G, Walt D. Detection of amyloid ß oligomers toward early diagnosis of Alzheimer's disease. Anal Biochem. 2019 02 01; 566:40-45. PMID: 30267709.
    Citations: 4     Fields:    Translation:Humans
  59. Cohen L, Walt DR. Highly Sensitive and Multiplexed Protein Measurements. Chem Rev. 2019 01 09; 119(1):293-321. PMID: 30152694.
    Citations: 27     Fields:    Translation:HumansAnimals
  60. Chen-Plotkin AS, Albin R, Alcalay R, Babcock D, Bajaj V, Bowman D, Buko A, Cedarbaum J, Chelsky D, Cookson MR, Dawson TM, Dewey R, Foroud T, Frasier M, German D, Gwinn K, Huang X, Kopil C, Kremer T, Lasch S, Marek K, Marto JA, Merchant K, Mollenhauer B, Naito A, Potashkin J, Reimer A, Rosenthal LS, Saunders-Pullman R, Scherzer CR, Sherer T, Singleton A, Sutherland M, Thiele I, van der Brug M, Van Keuren-Jensen K, Vaillancourt D, Walt D, West A, Zhang J. Finding useful biomarkers for Parkinson's disease. Sci Transl Med. 2018 08 15; 10(454). PMID: 30111645; PMCID: PMC6097233.
    Citations: 37     Fields:    Translation:Humans
  61. Li X, Jiang Y, Chong S, Walt DR. Bottom-up single-molecule strategy for understanding subunit function of tetrameric ß-galactosidase. Proc Natl Acad Sci U S A. 2018 08 14; 115(33):8346-8351. PMID: 30061400; PMCID: PMC6099853.
    Citations: 3     Fields:    Translation:Cells
  62. Duan BK, Cavanagh PE, Li X, Walt DR. Ultrasensitive Single-Molecule Enzyme Detection and Analysis Using a Polymer Microarray. Anal Chem. 2018 03 06; 90(5):3091-3098. PMID: 29425025.
    Citations: 6     Fields:    Translation:Cells
  63. Aebersold R, Agar JN, Amster IJ, Baker MS, Bertozzi CR, Boja ES, Costello CE, Cravatt BF, Fenselau C, Garcia BA, Ge Y, Gunawardena J, Hendrickson RC, Hergenrother PJ, Huber CG, Ivanov AR, Jensen ON, Jewett MC, Kelleher NL, Kiessling LL, Krogan NJ, Larsen MR, Loo JA, Ogorzalek Loo RR, Lundberg E, MacCoss MJ, Mallick P, Mootha VK, Mrksich M, Muir TW, Patrie SM, Pesavento JJ, Pitteri SJ, Rodriguez H, Saghatelian A, Sandoval W, Schlüter H, Sechi S, Slavoff SA, Smith LM, Snyder MP, Thomas PM, Uhlén M, Van Eyk JE, Vidal M, Walt DR, White FM, Williams ER, Wohlschlager T, Wysocki VH, Yates NA, Young NL, Zhang B. How many human proteoforms are there? Nat Chem Biol. 2018 02 14; 14(3):206-214. PMID: 29443976; PMCID: PMC5837046.
    Citations: 188     Fields:    Translation:HumansCells
  64. Khanna P, Maron JL, Walt DR. Development of a Rapid Salivary Proteomic Platform for Oral Feeding Readiness in the Preterm Newborn. Front Pediatr. 2017; 5:268. PMID: 29312906; PMCID: PMC5733069.
    Citations: 3     
  65. Cohen L, Xie L, Xylas ME, Walt DR. Single Molecule Arrays for ultra-sensitive detection of rat cytokines in serum. J Immunol Methods. 2018 01; 452:20-25. PMID: 29037955.
    Citations: 2     Fields:    Translation:Animals
  66. Wu D, Dinh TL, Bausk BP, Walt DR. Long-Term Measurements of Human Inflammatory Cytokines Reveal Complex Baseline Variations between Individuals. Am J Pathol. 2017 Dec; 187(12):2620-2626. PMID: 28919109.
    Citations: 12     Fields:    Translation:Humans
  67. Cohen L, Hartman MR, Amardey-Wellington A, Walt DR. Digital direct detection of microRNAs using single molecule arrays. Nucleic Acids Res. 2017 Aug 21; 45(14):e137. PMID: 28637221; PMCID: PMC5737668.
    Citations: 20     Fields:    Translation:Humans
  68. Gwinn K, David KK, Swanson-Fischer C, Albin R, Hillaire-Clarke CS, Sieber BA, Lungu C, Bowman FD, Alcalay RN, Babcock D, Dawson TM, Dewey RB, Foroud T, German D, Huang X, Petyuk V, Potashkin JA, Saunders-Pullman R, Sutherland M, Walt DR, West AB, Zhang J, Chen-Plotkin A, Scherzer CR, Vaillancourt DE, Rosenthal LS. Parkinson's disease biomarkers: perspective from the NINDS Parkinson's Disease Biomarkers Program. Biomark Med. 2017 May; 11(6):451-473. PMID: 28644039.
    Citations: 23     Fields:    Translation:Humans
  69. Barney BT, Munkholm C, Walt DR, Palumbi SR. Highly localized divergence within supergenes in Atlantic cod (Gadus morhua) within the Gulf of Maine. BMC Genomics. 2017 03 31; 18(1):271. PMID: 28359300; PMCID: PMC5374575.
    Citations: 21     Fields:    Translation:Animals
  70. Cohen L, Walt DR. Single-Molecule Arrays for Protein and Nucleic Acid Analysis. Annu Rev Anal Chem (Palo Alto Calif). 2017 06 12; 10(1):345-363. PMID: 28301748.
    Citations: 22     Fields:    Translation:Humans
  71. Yang X, Hartman MR, Harrington KT, Etson CM, Fierman MB, Slonim DK, Walt DR. Using Next-Generation Sequencing to Explore Genetics and Race in the High School Classroom. CBE Life Sci Educ. 2017; 16(2). PMID: 28408407.
    Citations: 5     Fields:    Translation:Humans
  72. Hartman MR, Harrington KT, Etson CM, Fierman MB, Slonim DK, Walt DR. Personal microbiomes and next-generation sequencing for laboratory-based education. FEMS Microbiol Lett. 2016 12; 363(23). PMID: 27856569; PMCID: PMC5827621.
    Citations: 6     Fields:    Translation:Humans
  73. Dinh TL, Ngan KC, Shoemaker CB, Walt DR. Using Antigen-antibody Binding Kinetic Parameters to Understand Single-Molecule Array Immunoassay Performance. Anal Chem. 2016 12 06; 88(23):11335-11339. PMID: 27779850.
    Citations: 3     Fields:    Translation:Cells
  74. Mogalisetti P, Walt DR. Enzyme Kinetics in Femtoliter Arrays. Methods Enzymol. 2016; 581:541-560. PMID: 27793293.
    Citations: 1     Fields:    
  75. Wu D, Katilius E, Olivas E, Dumont Milutinovic M, Walt DR. Incorporation of Slow Off-Rate Modified Aptamers Reagents in Single Molecule Array Assays for Cytokine Detection with Ultrahigh Sensitivity. Anal Chem. 2016 09 06; 88(17):8385-9. PMID: 27529794.
    Citations: 10     Fields:    Translation:Humans
  76. Schubert SM, Walter SR, Manesse M, Walt DR. Protein Counting in Single Cancer Cells. Anal Chem. 2016 Mar 01; 88(5):2952-7. PMID: 26813414.
    Citations: 11     Fields:    Translation:Humans
  77. Agre P, Bertozzi C, Bissell M, Campbell KP, Cummings RD, Desai UR, Estes M, Flotte T, Fogleman G, Gage F, Ginsburg D, Gordon JI, Hart G, Hascall V, Kiessling L, Kornfeld S, Lowe J, Magnani J, Mahal LK, Medzhitov R, Roberts RJ, Sackstein R, Sarkar R, Schnaar R, Schwartz N, Varki A, Walt D, Weissman I. Training the next generation of biomedical investigators in glycosciences. J Clin Invest. 2016 Feb; 126(2):405-8. PMID: 26829621; PMCID: PMC4731185.
    Citations: 15     Fields:    Translation:HumansAnimals
  78. Wu D, Milutinovic MD, Walt DR. Single molecule array (Simoa) assay with optimal antibody pairs for cytokine detection in human serum samples. Analyst. 2015 Sep 21; 140(18):6277-82. PMID: 26270328.
    Citations: 18     Fields:    Translation:Humans
  79. Nie S, Zhang H, Mayer KM, Oppenheim FG, Little FF, Greenberg J, Uluer AZ, Walt DR. Correlations of salivary biomarkers with clinical assessments in patients with cystic fibrosis. PLoS One. 2015; 10(8):e0135237. PMID: 26258476.
    Citations: 7     Fields:    Translation:Humans
  80. Sweedler JV, Armstrong DW, Baba Y, Desmet G, Dovichi N, Ewing A, Fenselau CC, Kennedy RT, Larive CK, Ligler FS, McCreery RL, Niessner R, Pemberton JE, Tan W, Walt DR, Yates JR, Zenobi R, Zhang X. The Scope of Analytical Chemistry. Anal Chem. 2015 Jul 07; 87(13):6425. PMID: 26086069.
    Citations: 2     Fields:    
  81. Gaylord ST, Dinh TL, Goldman ER, Anderson GP, Ngan KC, Walt DR. Ultrasensitive Detection of Ricin Toxin in Multiple Sample Matrixes Using Single-Domain Antibodies. Anal Chem. 2015 Jul 07; 87(13):6570-7. PMID: 26001138.
    Citations: 10     Fields:    
  82. Schubert SM, Arendt LM, Zhou W, Baig S, Walter SR, Buchsbaum RJ, Kuperwasser C, Walt DR. Ultra-sensitive protein detection via Single Molecule Arrays towards early stage cancer monitoring. Sci Rep. 2015 Jun 08; 5:11034. PMID: 26052106.
    Citations: 11     Fields:    Translation:HumansAnimalsCells
  83. Carrasco S, Benito-Peña E, Walt DR, Moreno-Bondi MC. Fiber-optic array using molecularly imprinted microspheres for antibiotic analysis. Chem Sci. 2015 May 01; 6(5):3139-3147. PMID: 29142687; PMCID: PMC5657405.
    Citations: 5     
  84. Khanna P, Walt DR. Salivary diagnostics using a portable point-of-service platform: a review. Clin Ther. 2015 Mar 01; 37(3):498-504. PMID: 25732629.
    Citations: 9     Fields:    Translation:Humans
  85. Gaylord ST, Abdul-Aziz S, Walt DR. Single-molecule arrays for ultrasensitive detection of host immune response to dengue virus infection. J Clin Microbiol. 2015 May; 53(5):1722-4. PMID: 25694521; PMCID: PMC4400757.
    Citations: 2     Fields:    Translation:HumansCells
  86. Mogalisetti P, Walt DR. Stoichiometry of the a-complementation reaction of Escherichia coli ß-galactosidase as revealed through single-molecule studies. Biochemistry. 2015 Mar 03; 54(8):1583-8. PMID: 25668156.
    Citations: 4     Fields:    Translation:Cells
  87. Mayer KM, Shnipes J, Davis D, Walt DR. Catalytic kinetics of single gold nanoparticles observed via optical microwell arrays. Nanotechnology. 2015 Feb 06; 26(5):055704. PMID: 25590419.
    Citations: 1     Fields:    
  88. Raz N, Danin-Poleg Y, Hayman RB, Bar-On Y, Linetsky A, Shmoish M, Sanjuán E, Amaro C, Walt DR, Kashi Y. Genome-wide SNP-genotyping array to study the evolution of the human pathogen Vibrio vulnificus biotype 3. PLoS One. 2014; 9(12):e114576. PMID: 25526263.
    Citations: 11     Fields:    Translation:Cells
  89. Kelley SO, Mirkin CA, Walt DR, Ismagilov RF, Toner M, Sargent EH. Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering. Nat Nanotechnol. 2014 Dec; 9(12):969-80. PMID: 25466541.
    Citations: 80     Fields:    Translation:Humans
  90. Warren AD, Gaylord ST, Ngan KC, Dumont Milutinovic M, Kwong GA, Bhatia SN, Walt DR. Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers. J Am Chem Soc. 2014 Oct 01; 136(39):13709-14. PMID: 25198059.
    Citations: 20     Fields:    Translation:Animals
  91. Walt DR. Protein measurements in microwells. Lab Chip. 2014 Sep 07; 14(17):3195-200. PMID: 24825470.
    Citations: 7     Fields:    
  92. Nie S, Henley WH, Miller SE, Zhang H, Mayer KM, Dennis PJ, Oblath EA, Alarie JP, Wu Y, Oppenheim FG, Little FF, Uluer AZ, Wang P, Ramsey JM, Walt DR. An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples. Lab Chip. 2014 Mar 21; 14(6):1087-98. PMID: 24448498.
    Citations: 25     Fields:    Translation:HumansCTClinical Trials
  93. Rojek MJ, Walt DR. Observing single enzyme molecules interconvert between activity states upon heating. PLoS One. 2014; 9(1):e86224. PMID: 24465972.
    Citations: 3     Fields:    Translation:Cells
  94. Little FF, Delgado DM, Wexler PJ, Oppenheim FG, Mitchell P, Feldman JA, Walt DR, Peng RD, Matsui EC. Salivary inflammatory mediator profiling and correlation to clinical disease markers in asthma. PLoS One. 2014; 9(1):e84449. PMID: 24409298.
    Citations: 17     Fields:    Translation:Humans
  95. Nie S, Benito-Peña E, Zhang H, Wu Y, Walt DR. Multiplexed fluorescent microarray for human salivary protein analysis using polymer microspheres and fiber-optic bundles. J Vis Exp. 2013 Oct 10; (80). PMID: 24145242.
    Citations: 2     Fields:    Translation:Humans
  96. Nie S, Benito-Peña E, Zhang H, Wu Y, Walt DR. Multiplexed salivary protein profiling for patients with respiratory diseases using fiber-optic bundles and fluorescent antibody-based microarrays. Anal Chem. 2013 Oct 01; 85(19):9272-80. PMID: 23972398.
    Citations: 11     Fields:    Translation:Humans
  97. LaFratta CN, Pelse I, Falla JL, Liu Y, Palacios MA, Manesse M, Whitesides GM, Walt DR. Measuring atomic emission from beacons for long-distance chemical signaling. Anal Chem. 2013 Oct 01; 85(19):8933-6. PMID: 23987667.
    Citations:    Fields:    
  98. Manesse M, Phillips AF, LaFratta CN, Palacios MA, Hayman RB, Walt DR. Dynamic microbead arrays for biosensing applications. Lab Chip. 2013 Jun 07; 13(11):2153-60. PMID: 23615790.
    Citations: 4     Fields:    Translation:Cells
  99. Palacios MA, Lacy MM, Schubert SM, Manesse M, Walt DR. Assessing the stochastic intermittency of single quantum dot luminescence for robust quantification of biomolecules. Anal Chem. 2013 Jul 16; 85(14):6639-45. PMID: 23631644.
    Citations:    Fields:    
  100. Song L, Shan D, Zhao M, Pink BA, Minnehan KA, York L, Gardel M, Sullivan S, Phillips AF, Hayman RB, Walt DR, Duffy DC. Direct detection of bacterial genomic DNA at sub-femtomolar concentrations using single molecule arrays. Anal Chem. 2013 Feb 05; 85(3):1932-9. PMID: 23331316.
    Citations: 27     Fields:    Translation:Humans
  101. Walt DR. Optical methods for single molecule detection and analysis. Anal Chem. 2013 Feb 05; 85(3):1258-63. PMID: 23215010.
    Citations: 38     Fields:    
  102. Albilia JB, Tenenbaum HC, Clokie CM, Walt DR, Baker GI, Psutka DJ, Backstein D, Peel SA. Serum levels of BMP-2, 4, 7 and AHSG in patients with degenerative joint disease requiring total arthroplasty of the hip and temporomandibular joints. J Orthop Res. 2013 Jan; 31(1):44-52. PMID: 22778059.
    Citations: 14     Fields:    Translation:Humans
  103. Zhang H, Nie S, Etson CM, Wang RM, Walt DR. Oil-sealed femtoliter fiber-optic arrays for single molecule analysis. Lab Chip. 2012 Jun 21; 12(12):2229-39. PMID: 22311152.
    Citations: 10     Fields:    
  104. Anderson WA, Amasino RM, Ares M, Banerjee U, Bartel B, Corces VG, Drennan CL, Elgin SC, Epstein IR, Fanning E, Guillette LJ, Handelsman J, Hatfull GF, Hoy RR, Kelley D, Leinwand LA, Losick R, Lu Y, Lynn DG, Neuhauser C, O'Dowd DK, Olivera T, Pevzner P, Richards-Kortum RR, Rine J, Sah RL, Strobel SA, Walker GC, Walt DR, Warner IM, Wessler S, Willard HF, Zare RN. Competencies: a cure for pre-med curriculum. Science. 2011 Nov 11; 334(6057):760-1. PMID: 22076362.
    Citations: 1     Fields:    
  105. Palacios MA, Benito-Peña E, Manesse M, Mazzeo AD, Lafratta CN, Whitesides GM, Walt DR. InfoBiology by printed arrays of microorganism colonies for timed and on-demand release of messages. Proc Natl Acad Sci U S A. 2011 Oct 04; 108(40):16510-4. PMID: 21949364.
    Citations: 6     Fields:    Translation:Cells
  106. Thomadaki K, Helmerhorst EJ, Tian N, Sun X, Siqueira WL, Walt DR, Oppenheim FG. Whole-saliva proteolysis and its impact on salivary diagnostics. J Dent Res. 2011 Nov; 90(11):1325-30. PMID: 21917601; PMCID: PMC3188460.
    Citations: 37     Fields:    Translation:HumansCells
  107. Stitzel SE, Aernecke MJ, Walt DR. Artificial noses. Annu Rev Biomed Eng. 2011 Aug 15; 13:1-25. PMID: 21417721.
    Citations: 23     Fields:    Translation:HumansAnimals
  108. Whitaker RD, Pember S, Wallace BC, Brodley CE, Walt DR. Single cell time-resolved quorum responses reveal dependence on cell density and configuration. J Biol Chem. 2011 Jun 17; 286(24):21623-32. PMID: 21527637.
    Citations: 2     Fields:    Translation:Cells
  109. Walt DR, Kuhlik A, Epstein SK, Demmer LA, Knight M, Chelmow D, Rosenblatt M, Bianchi DW. Lessons learned from the introduction of personalized genotyping into a medical school curriculum. Genet Med. 2011 Jan; 13(1):63-6. PMID: 21057320.
    Citations: 28     Fields:    Translation:Humans
  110. Lafratta CN, Huh SP, Mallillin AC, Riviello PJ, Walt DR. Visualizing Fluorescence: Using a Homemade Fluorescence "Microscope" to View Latent Fingerprints on Paper. J Chem Educ. 2010 Oct 01; 87(10):1105-1107. PMID: 20852733.
    Citations:    Fields:    
  111. Walt DR. Bead-based optical fiber arrays for artificial olfaction. Curr Opin Chem Biol. 2010 Dec; 14(6):767-70. PMID: 20655803.
    Citations: 4     Fields:    Translation:HumansAnimals
  112. Gorris HH, Walt DR. Analytical chemistry on the femtoliter scale. Angew Chem Int Ed Engl. 2010 May 25; 49(23):3880-95. PMID: 20419719.
    Citations: 12     Fields:    
  113. Rissin DM, Kan CW, Campbell TG, Howes SC, Fournier DR, Song L, Piech T, Patel PP, Chang L, Rivnak AJ, Ferrell EP, Randall JD, Provuncher GK, Walt DR, Duffy DC. Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations. Nat Biotechnol. 2010 Jun; 28(6):595-9. PMID: 20495550.
    Citations: 489     Fields:    Translation:Humans
  114. Faustov AR, Webb MR, Walt DR. Note: Toward multiple addressable optical trapping. Rev Sci Instrum. 2010 Feb; 81(2):026109. PMID: 20192526.
    Citations: 1     Fields:    
  115. Aernecke MJ, Walt DR. Detection and classification of ignitable liquid residues using a fluorescence-based vapor-sensitive microsphere array. J Forensic Sci. 2010 Jan; 55(1):178-84. PMID: 20002259.
    Citations: 1     Fields:    
  116. Walt DR. Fibre optic microarrays. Chem Soc Rev. 2010 Jan; 39(1):38-50. PMID: 20023835.
    Citations: 25     Fields:    Translation:HumansAnimals
  117. Walt DR. Ubiquitous sensors: when will they be here? ACS Nano. 2009 Oct 27; 3(10):2876-80. PMID: 19856977.
    Citations: 6     Fields:    
  118. Konry T, Walt DR. Intelligent medical diagnostics via molecular logic. J Am Chem Soc. 2009 Sep 23; 131(37):13232-3. PMID: 19715272.
    Citations: 16     Fields:    Translation:Humans
  119. Webb MR, LaFratta CN, Walt DR. Chromatically resolved optical microscope (CROMoscope): a grating-based instrument for spectral imaging. Anal Chem. 2009 Sep 01; 81(17):7309-13. PMID: 19653690.
    Citations: 1     Fields:    Translation:AnimalsCells
  120. Aernecke MJ, Walt DR. Temporally resolved fluorescence spectroscopy of a microarray-based vapor sensing system. Anal Chem. 2009 Jul 15; 81(14):5762-9. PMID: 19518137.
    Citations: 2     Fields:    Translation:Cells
  121. Konry T, Hayman RB, Walt DR. Microsphere-based rolling circle amplification microarray for the detection of DNA and proteins in a single assay. Anal Chem. 2009 Jul 15; 81(14):5777-82. PMID: 19548682.
    Citations: 17     Fields:    Translation:Humans
  122. Aernecke MJ, Guo J, Sonkusale S, Walt DR. Design, implementation, and field testing of a portable fluorescence-based vapor sensor. Anal Chem. 2009 Jul 01; 81(13):5281-90. PMID: 19563211.
    Citations: 7     Fields:    
  123. Thomas SW, Chiechi RC, LaFratta CN, Webb MR, Lee A, Wiley BJ, Zakin MR, Walt DR, Whitesides GM. Infochemistry and infofuses for the chemical storage and transmission of coded information. Proc Natl Acad Sci U S A. 2009 Jun 09; 106(23):9147-50. PMID: 19470465.
    Citations: 6     Fields:    
  124. Deiss F, LaFratta CN, Symer M, Blicharz TM, Sojic N, Walt DR. Multiplexed sandwich immunoassays using electrochemiluminescence imaging resolved at the single bead level. J Am Chem Soc. 2009 May 06; 131(17):6088-9. PMID: 19361216.
    Citations: 17     Fields:    
  125. Gorris HH, Walt DR. Mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies. J Am Chem Soc. 2009 May 06; 131(17):6277-82. PMID: 19338338.
    Citations: 35     Fields:    
  126. Blicharz TM, Siqueira WL, Helmerhorst EJ, Oppenheim FG, Wexler PJ, Little FF, Walt DR. Fiber-optic microsphere-based antibody array for the analysis of inflammatory cytokines in saliva. Anal Chem. 2009 Mar 15; 81(6):2106-14. PMID: 19192965.
    Citations: 34     Fields:    Translation:Humans
  127. Tam JM, Song L, Walt DR. DNA detection on ultrahigh-density optical fiber-based nanoarrays. Biosens Bioelectron. 2009 Apr 15; 24(8):2488-93. PMID: 19195871.
    Citations: 6     Fields:    
  128. Kelley SO, Walt DR. Measurement science: the engine of chemical biology. Curr Opin Chem Biol. 2008 Oct; 12(5):473-4. PMID: 18782631.
    Citations:    Fields:    
  129. Li Z, Hayman RB, Walt DR. Detection of single-molecule DNA hybridization using enzymatic amplification in an array of femtoliter-sized reaction vessels. J Am Chem Soc. 2008 Sep 24; 130(38):12622-3. PMID: 18763768.
    Citations: 13     Fields:    Translation:Cells
  130. Blicharz TM, Rissin DM, Bowden M, Hayman RB, DiCesare C, Bhatia JS, Grand-Pierre N, Siqueira WL, Helmerhorst EJ, Loscalzo J, Oppenheim FG, Walt DR. Use of colorimetric test strips for monitoring the effect of hemodialysis on salivary nitrite and uric acid in patients with end-stage renal disease: a proof of principle. Clin Chem. 2008 Sep; 54(9):1473-80. PMID: 18676588.
    Citations: 16     Fields:    Translation:HumansCells
  131. Rissin DM, Gorris HH, Walt DR. Distinct and long-lived activity states of single enzyme molecules. J Am Chem Soc. 2008 Apr 16; 130(15):5349-53. PMID: 18318491.
    Citations: 19     Fields:    Translation:Cells
  132. LaFratta CN, Walt DR. Very high density sensing arrays. Chem Rev. 2008 Feb; 108(2):614-37. PMID: 18229955.
    Citations: 34     Fields:    
  133. Bake KD, Walt DR. Multiplexed spectroscopic detections. Annu Rev Anal Chem (Palo Alto Calif). 2008; 1:515-47. PMID: 20636088.
    Citations: 4     Fields:    Translation:HumansAnimals
  134. Whitaker RD, Walt DR. Multianalyte single-cell analysis with multiple cell lines using a fiber-optic array. Anal Chem. 2007 Dec 01; 79(23):9045-53. PMID: 17973503.
    Citations: 4     Fields:    Translation:HumansCells
  135. Gorris HH, Rissin DM, Walt DR. Stochastic inhibitor release and binding from single-enzyme molecules. Proc Natl Acad Sci U S A. 2007 Nov 06; 104(45):17680-5. PMID: 17965235.
    Citations: 21     Fields:    
  136. Gorris HH, Blicharz TM, Walt DR. Optical-fiber bundles. FEBS J. 2007 Nov; 274(21):5462-70. PMID: 17937772.
    Citations: 15     Fields:    
  137. Walt DR. Smells like bread. Nat Chem Biol. 2007 Jun; 3(6):306-7. PMID: 17510645.
    Citations:    Fields:    Translation:AnimalsCells
  138. Walt DR, Blicharz TM, Hayman RB, Rissin DM, Bowden M, Siqueira WL, Helmerhorst EJ, Grand-Pierre N, Oppenheim FG, Bhatia JS, Little FF, Brody JS. Microsensor arrays for saliva diagnostics. Ann N Y Acad Sci. 2007 Mar; 1098:389-400. PMID: 17435144; PMCID: PMC7168095.
    Citations: 15     Fields:    Translation:Humans
  139. Kuang Y, Walt DR. Detecting oxygen consumption in the proximity of Saccharomyces cerevisiae cells using self-assembled fluorescent nanosensors. Biotechnol Bioeng. 2007 Feb 01; 96(2):318-25. PMID: 16878334.
    Citations: 6     Fields:    Translation:Animals
  140. Blake WJ, Balázsi G, Kohanski MA, Isaacs FJ, Murphy KF, Kuang Y, Cantor CR, Walt DR, Collins JJ. Phenotypic consequences of promoter-mediated transcriptional noise. Mol Cell. 2006 Dec 28; 24(6):853-65. PMID: 17189188.
    Citations: 283     Fields:    Translation:AnimalsCells
  141. Walt DR. Fiber optic array biosensors. Biotechniques. 2006 Nov; 41(5):529, 531, 533 passim. PMID: 17140107.
    Citations: 3     Fields:    Translation:HumansAnimals
  142. Whitaker RD, Walt DR. Fiber-based single cell analysis of reporter gene expression in yeast two-hybrid systems. Anal Biochem. 2007 Jan 01; 360(1):63-74. PMID: 17113561.
    Citations: 3     Fields:    Translation:Animals
  143. Ahn S, Kulis DM, Erdner DL, Anderson DM, Walt DR. Fiber-optic microarray for simultaneous detection of multiple harmful algal bloom species. Appl Environ Microbiol. 2006 Sep; 72(9):5742-9. PMID: 16957189; PMCID: PMC1563625.
    Citations: 13     Fields:    Translation:AnimalsCells
  144. Rissin DM, Walt DR. Digital readout of target binding with attomole detection limits via enzyme amplification in femtoliter arrays. J Am Chem Soc. 2006 May 17; 128(19):6286-7. PMID: 16683771.
    Citations: 23     Fields:    
  145. Bencic-Nagale S, Sternfeld T, Walt DR. Microbead chemical switches: an approach to detection of reactive organophosphate chemical warfare agent vapors. J Am Chem Soc. 2006 Apr 19; 128(15):5041-8. PMID: 16608338.
    Citations: 4     Fields:    
  146. Rissin DM, Walt DR. Digital concentration readout of single enzyme molecules using femtoliter arrays and Poisson statistics. Nano Lett. 2006 Mar; 6(3):520-3. PMID: 16522055.
    Citations: 34     Fields:    
  147. Song L, Ahn S, Walt DR. Fiber-optic microsphere-based arrays for multiplexed biological warfare agent detection. Anal Chem. 2006 Feb 15; 78(4):1023-33. PMID: 16478092.
    Citations: 11     Fields:    
  148. Rissin DM, Walt DR. Duplexed sandwich immunoassays on a fiber-optic microarray. Anal Chim Acta. 2006 Mar 30; 564(1):34-9. PMID: 17723359.
    Citations: 8     Fields:    
  149. Kuang Y, Walt DR. Monitoring "promiscuous" drug effects on single cells of multiple cell types. Anal Biochem. 2005 Oct 15; 345(2):320-5. PMID: 16099418.
    Citations: 4     Fields:    Translation:Cells
  150. Bencic-Nagale S, Walt DR. Extending the longevity of fluorescence-based sensor arrays using adaptive exposure. Anal Chem. 2005 Oct 01; 77(19):6155-62. PMID: 16194073.
    Citations: 2     Fields:    
  151. Song L, Ahn S, Walt DR. Detecting biological warfare agents. Emerg Infect Dis. 2005 Oct; 11(10):1629-32. PMID: 16318712; PMCID: PMC3402143.
    Citations: 8     Fields:    Translation:HumansCells
  152. Brogan KL, Walt DR. Optical fiber-based sensors: application to chemical biology. Curr Opin Chem Biol. 2005 Oct; 9(5):494-500. PMID: 16125439.
    Citations: 7     Fields:    Translation:Cells
  153. Bowden M, Song L, Walt DR. Development of a microfluidic platform with an optical imaging microarray capable of attomolar target DNA detection. Anal Chem. 2005 Sep 01; 77(17):5583-8. PMID: 16131069.
    Citations: 9     Fields:    Translation:Cells
  154. Ahn S, Walt DR. Detection of Salmonella spp. using microsphere-based, fiber-optic DNA microarrays. Anal Chem. 2005 Aug 01; 77(15):5041-7. PMID: 16053320.
    Citations: 13     Fields:    Translation:CellsPHPublic Health
  155. Tam JM, Song L, Walt DR. Fabrication and optical characterization of imaging fiber-based nanoarrays. Talanta. 2005 Sep 15; 67(3):498-502. PMID: 18970195.
    Citations:    Fields:    
  156. Weis BK, Balshaw D, Barr JR, Brown D, Ellisman M, Lioy P, Omenn G, Potter JD, Smith MT, Sohn L, Suk WA, Sumner S, Swenberg J, Walt DR, Watkins S, Thompson C, Wilson SH. Personalized exposure assessment: promising approaches for human environmental health research. Environ Health Perspect. 2005 Jul; 113(7):840-8. PMID: 16002370;