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Alain Charest, Ph.D.

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Mentoring
Available: 10/01/18, Expires: 08/31/21

Using genetically engineered mouse models of glioma, we aim to determine the effects of standard of care therapy on the intratumoral immune composition and function. We take a direct approach to this question by using state of the art single cell RNAseq methods and analyses. The student role will be to take ownership of mouse glioma isolation and single cell processing procedures and immerse themselves in downstream bioinformatics analysis.


Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. R21CA245337 (CHAREST, ALAIN) Dec 1, 2019 - Nov 30, 2021
    NIH/NCI
    Therapeutic vulnerabilities associated with PTEN missense mutations
    Role: Principal Investigator
  2. UG3CA241685 (COFFEY, ROBERT J.) Jul 15, 2019 - Jun 30, 2021
    NIH/NCI
    Uncovering exRNA and protein determinants of secreted vesicle heterogeneity by flow cytometric purification of vesicle subsets from cells and plasma
    Role: Co-Principal Investigator
  3. R01CA229784 (CHAREST, ALAIN) Jul 1, 2018 - Jun 30, 2023
    NIH/NCI
    Advancing treatment outcomes in malignant glioma by integrating immunotherapy and standard of care using genetically engineered mice that recapitulate molecular feature of human glioma
    Role: Principal Investigator
  4. R01CA185137 (CHAREST, ALAIN) Sep 1, 2014 - Aug 31, 2018
    NIH/NCI
    (PQB5)Molecular Wiring and Therapeutic Targeting of EGFR and PDGFR Signaling Netw
    Role: Principal Investigator
  5. U19CA179563 (BREAKEFIELD, XANDRA OWENS) Sep 1, 2013 - Aug 31, 2018
    NIH/NCI
    exRNA released by glioblastoma alters brain microenvironment
    Role: Co-Principal Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Bardhan K, Aksoylar HI, Le Bourgeois T, Strauss L, Weaver JD, Delcuze B, Charest A, Patsoukis N, Boussiotis VA. Publisher Correction: Phosphorylation of PD-1-Y248 is a marker of PD-1-mediated inhibitory function in human T cells. Sci Rep. 2020 Sep 23; 10(1):15905. PMID: 32963251.
    Citations:    
  2. Charest A. Experimental and Biological Insights from Proteomic Analyses of Extracellular Vesicle Cargos in Normalcy and Disease. Adv Biosyst. 2020 Aug 19; e2000069. PMID: 32815324.
    Citations:    
  3. Zeng A, Wei Z, Rabinovsky R, Jun HJ, El Fatimy R, Deforzh E, Arora R, Yao Y, Yao S, Yan W, Uhlmann EJ, Charest A, You Y, Krichevsky AM. Glioblastoma-Derived Extracellular Vesicles Facilitate Transformation of Astrocytes via Reprogramming Oncogenic Metabolism. iScience. 2020 Aug 21; 23(8):101420. PMID: 32795915.
    Citations:    
  4. Bardhan K, Aksoylar HI, Le Bourgeois T, Strauss L, Weaver JD, Delcuze B, Charest A, Patsoukis N, Boussiotis VA. Phosphorylation of PD-1-Y248 is a marker of PD-1-mediated inhibitory function in human T cells. Sci Rep. 2019 11 21; 9(1):17252. PMID: 31754127.
    Citations:    
  5. Jones PS, Yekula A, Lansbury E, Small JL, Ayinon C, Mordecai S, Hochberg FH, Tigges J, Delcuze B, Charest A, Ghiran I, Balaj L, Carter BS. Characterization of plasma-derived protoporphyrin-IX-positive extracellular vesicles following 5-ALA use in patients with malignant glioma. EBioMedicine. 2019 Oct; 48:23-35. PMID: 31628025.
    Citations:    
  6. Gyuris A, Navarrete-Perea J, Jo A, Cristea S, Zhou S, Fraser K, Wei Z, Krichevsky AM, Weissleder R, Lee H, Gygi SP, Charest A. Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity. Cell Rep. 2019 06 25; 27(13):3972-3987.e6. PMID: 31242427.
    Citations:    
  7. Das S, Ansel KM, Bitzer M, Breakefield XO, Charest A, Galas DJ, Gerstein MB, Gupta M, Milosavljevic A, McManus MT, Patel T, Raffai RL, Rozowsky J, Roth ME, Saugstad JA, Van Keuren-Jensen K, Weaver AM, Laurent LC. The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research. Cell. 2019 04 04; 177(2):231-242. PMID: 30951667.
    Citations:    
  8. Jun HJ, Appleman VA, Wu HJ, Rose CM, Pineda JJ, Yeo AT, Delcuze B, Lee C, Gyuris A, Zhu H, Woolfenden S, Bronisz A, Nakano I, Chiocca EA, Bronson RT, Ligon KL, Sarkaria JN, Gygi SP, Michor F, Mitchison TJ, Charest A. A PDGFRa-driven mouse model of glioblastoma reveals a stathmin1-mediated mechanism of sensitivity to vinblastine. Nat Commun. 2018 08 06; 9(1):3116. PMID: 30082792.
    Citations:    Fields:    Translation:HumansAnimalsCells
  9. Zhou S, Appleman VA, Rose CM, Jun HJ, Yang J, Zhou Y, Bronson RT, Gygi SP, Charest A. Chronic platelet-derived growth factor receptor signaling exerts control over initiation of protein translation in glioma. Life Sci Alliance. 2018 Jun; 1(3):e201800029. PMID: 30456354.
    Citations:    
  10. Boussiotis VA, Charest A. Immunotherapies for malignant glioma. Oncogene. 2018 03; 37(9):1121-1141. PMID: 29242608.
    Citations: 2     Fields:    
  11. Yeo AT, Charest A. Immune Checkpoint Blockade Biology in Mouse Models of Glioblastoma. J Cell Biochem. 2017 09; 118(9):2516-2527. PMID: 28230277.
    Citations: 2     Fields:    Translation:HumansAnimals
  12. Ricklefs F, Mineo M, Rooj AK, Nakano I, Charest A, Weissleder R, Breakefield XO, Chiocca EA, Godlewski J, Bronisz A. Extracellular Vesicles from High-Grade Glioma Exchange Diverse Pro-oncogenic Signals That Maintain Intratumoral Heterogeneity. Cancer Res. 2016 05 15; 76(10):2876-81. PMID: 27013191.
    Citations: 15     Fields:    Translation:HumansAnimalsCells
  13. Ali ZA, de Jesus Perez V, Yuan K, Orcholski M, Pan S, Qi W, Chopra G, Adams C, Kojima Y, Leeper NJ, Qu X, Zaleta-Rivera K, Kato K, Yamada Y, Oguri M, Kuchinsky A, Hazen SL, Jukema JW, Ganesh SK, Nabel EG, Channon K, Leon MB, Charest A, Quertermous T, Ashley EA. Oxido-reductive regulation of vascular remodeling by receptor tyrosine kinase ROS1. J Clin Invest. 2014 Dec; 124(12):5159-74. PMID: 25401476.
    Citations: 8     Fields:    Translation:HumansAnimalsCells
  14. Jun HJ, Roy J, Smith TB, Wood LB, Lane K, Woolfenden S, Punko D, Bronson RT, Haigis KM, Breton S, Charest A. ROS1 signaling regulates epithelial differentiation in the epididymis. Endocrinology. 2014 Sep; 155(9):3661-73. PMID: 24971615.
    Citations: 5     Fields:    Translation:AnimalsCells
  15. Jun HJ, Bronson RT, Charest A. Inhibition of EGFR induces a c-MET-driven stem cell population in glioblastoma. Stem Cells. 2014 Feb; 32(2):338-48. PMID: 24115218.
    Citations: 21     Fields:    Translation:HumansAnimalsCells
  16. Shao H, Chung J, Balaj L, Charest A, Bigner DD, Carter BS, Hochberg FH, Breakefield XO, Weissleder R, Lee H. Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy. Nat Med. 2012 Dec; 18(12):1835-40. PMID: 23142818.
    Citations: 141     Fields:    Translation:HumansCells
  17. Jun HJ, Johnson H, Bronson RT, de Feraudy S, White F, Charest A. The oncogenic lung cancer fusion kinase CD74-ROS activates a novel invasiveness pathway through E-Syt1 phosphorylation. Cancer Res. 2012 Aug 01; 72(15):3764-74. PMID: 22659450.
    Citations: 19     Fields:    Translation:HumansAnimalsCells
  18. Agrawal A, Min DH, Singh N, Zhu H, Birjiniuk A, von Maltzahn G, Harris TJ, Xing D, Woolfenden SD, Sharp PA, Charest A, Bhatia S. Functional delivery of siRNA in mice using dendriworms. ACS Nano. 2009 Sep 22; 3(9):2495-504. PMID: 19673534.
    Citations: 37     Fields:    Translation:HumansAnimalsCells
  19. McCarty JH, Lacy-Hulbert A, Charest A, Bronson RT, Crowley D, Housman D, Savill J, Roes J, Hynes RO. Selective ablation of alphav integrins in the central nervous system leads to cerebral hemorrhage, seizures, axonal degeneration and premature death. Development. 2005 Jan; 132(1):165-76. PMID: 15576410.
    Citations: 97     Fields:    Translation:HumansAnimalsCells
  20. Charest A, Lane K, McMahon K, Park J, Preisinger E, Conroy H, Housman D. Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21). Genes Chromosomes Cancer. 2003 May; 37(1):58-71. PMID: 12661006.
    Citations: 65     Fields:    Translation:HumansAnimalsCells
  21. Jordan B, Charest A, Dowd JF, Blumenstiel JP, Yeh Rf RF, Osman A, Housman DE, Landers JE. Genome complexity reduction for SNP genotyping analysis. Proc Natl Acad Sci U S A. 2002 Mar 05; 99(5):2942-7. PMID: 11880640.
    Citations: 10     Fields:    Translation:HumansAnimals
  22. Heesen M, Berman MA, Charest A, Housman D, Gerard C, Dorf ME. Cloning and chromosomal mapping of an orphan chemokine receptor: mouse RDC1. Immunogenetics. 1998 Apr; 47(5):364-70. PMID: 9510554.
    Citations: 16     Fields:    Translation:HumansAnimalsCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.