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Giuseppe Di Caprio, Ph.D.

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University of Naples Federico II, Napoli (Italy)MS07/2006Physics (cum laude)
University of Naples Federico II, Napoli (Italy)PhD12/2014Novel Technologies for Imaging (cum laude)
2013 - 2014
Best Paper award: “Hyperspectral Microscopy of Flowing Cells” - OSA Imaging and Applied Optics
2018 - 2027
National Academic Qualification as Associate Professor of Physics applied to Biology and Medicine

My research bridges Optical Microscopy, Biology and Image Processing. I am interested in mechanistic questions addressed at a molecular level aided by fluorescence microscopy imaging acquired with high temporal and spatial resolution. During the past years I focused on problems associated with viral particles endocytosis and fusion processes, in-vitro assembly of clathrin coated pits, the shape of intracellular energy gradients in living cells, Pyrin-mediated inflammasome assembly and activation, and the mechanisms that regulates cell internalization of Transferrin-decorated DNA origami barrels. Aspects of my studies involve close collaborations with other members in the Kirchhausen group and with external collaborators. Most of the imaging is carried using minimally invasive, 3D live-cell lattice light-sheet microscopy (LLSM); I have also been responsible for implementing the construction of one of these microscopes in the Kirchhausen lab as well as developing the computational processing tools used for the quantitative analysis of the data generated by myself and by other members of our group.

My second appointment is at the Massachusetts General Hospital, in collaboration with John Higgins, MD, and David Williams MD, where my research is dedicated to the development of material technologies for mimicking the environment of the circulatory system in vitro. This method provides biomarkers promising to improve management of sickle cell patients and to optimize the development and prioritization of candidate cures, and it’s currently used to validate the efficacy of BCL11A as therapeutic target in Sickle Cell Disease, Clinical Trials Identifier: NCT03282656 (Sponsor: David Williams – Boston Children’s Hospital).

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Kreutzberger AJB, Sanyal A, Saminathan A, Bloyet LM, Stumpf S, Liu Z, Ojha R, Patjas MT, Geneid A, Scanavachi G, Doyle CA, Somerville E, Correia RBDC, Di Caprio G, Toppila-Salmi S, Mäkitie A, Kiessling V, Vapalahti O, Whelan SPJ, Balistreri G, Kirchhausen T. SARS-CoV-2 requires acidic pH to infect cells. Proc Natl Acad Sci U S A. 2022 09 20; 119(38):e2209514119. PMID: 36048924; PMCID: PMC9499588.
    Citations: 1     Fields:    Translation:HumansCells
  2. Kreutzberger AJB, Sanyal A, Saminathan A, Bloyet LM, Stumpf S, Liu Z, Ojha R, Patjas MT, Geneid A, Scanavachi G, Doyle CA, Somerville E, Bango Da Cunha Correira R, Di Caprio G, Toppila-Salmi S, Mäkitie A, Kiessling V, Vapalahti O, Whelan SPJ, Balistreri G, Kirchhausen T. SARS-CoV-2 requires acidic pH to infect cells. bioRxiv. 2022 Jun 14. PMID: 35702155; PMCID: PMC9196115.
  3. Chou YY, Upadhyayula S, Houser J, He K, Skillern W, Scanavachi G, Dang S, Sanyal A, Ohashi KG, Di Caprio G, Kreutzberger AJB, Vadakkan TJ, Kirchhausen T. Inherited nuclear pore substructures template post-mitotic pore assembly. Dev Cell. 2021 06 21; 56(12):1786-1803.e9. PMID: 34129835; PMCID: PMC8261643.
    Citations: 3     Fields:    Translation:HumansCells
  4. Demers M, Sturtevant S, Guertin KR, Gupta D, Desai K, Vieira BF, Li W, Hicks A, Ismail A, Gonçalves BP, Di Caprio G, Schonbrun E, Hansen S, Musayev FN, Safo MK, Wood DK, Higgins JM, Light DR. MetAP2 inhibition modifies hemoglobin S to delay polymerization and improves blood flow in sickle cell disease. Blood Adv. 2021 03 09; 5(5):1388-1402. PMID: 33661300.
    Citations:    Fields:    Translation:HumansAnimalsCells
  5. Salman MM, Marsh G, Kusters I, Delincé M, Di Caprio G, Upadhyayula S, de Nola G, Hunt R, Ohashi KG, Gray T, Shimizu F, Sano Y, Kanda T, Obermeier B, Kirchhausen T. Design and Validation of a Human Brain Endothelial Microvessel-on-a-Chip Open Microfluidic Model Enabling Advanced Optical Imaging. Front Bioeng Biotechnol. 2020; 8:573775. PMID: 33117784.
    Citations: 50     
  6. Magupalli VG, Negro R, Tian Y, Hauenstein AV, Di Caprio G, Skillern W, Deng Q, Orning P, Alam HB, Maliga Z, Sharif H, Hu JJ, Evavold CL, Kagan JC, Schmidt FI, Fitzgerald KA, Kirchhausen T, Li Y, Wu H. HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin inflammasome activation. Science. 2020 09 18; 369(6510). PMID: 32943500.
    Citations: 55     Fields:    Translation:HumansAnimalsCells
  7. Di Caprio G, Schonbrun E, Gonçalves BP, Valdez JM, Wood DK, Higgins JM. High-throughput assessment of hemoglobin polymer in single red blood cells from sickle cell patients under controlled oxygen tension. Proc Natl Acad Sci U S A. 2019 12 10; 116(50):25236-25242. PMID: 31767751; PMCID: PMC6911208.
    Citations: 9     Fields:    Translation:HumansCells
  8. Schuler MH, Lewandowska A, Caprio GD, Skillern W, Upadhyayula S, Kirchhausen T, Shaw JM, Cunniff B. Miro1-mediated mitochondrial positioning shapes intracellular energy gradients required for cell migration. Mol Biol Cell. 2017 Aug 01; 28(16):2159-2169. PMID: 28615318; PMCID: PMC5531732.
    Citations: 58     Fields:    Translation:AnimalsCells
  9. Schonbrun E, Di Caprio G. A virtually imaged defocused array (VIDA) for high-speed 3D microscopy. J Biophotonics. 2016 10; 9(10):1044-1049. PMID: 26694084.
    Citations:    Fields:    Translation:HumansCells
  10. Di Caprio G, Stokes C, Higgins JM, Schonbrun E. Single-cell measurement of red blood cell oxygen affinity. Proc Natl Acad Sci U S A. 2015 Aug 11; 112(32):9984-9. PMID: 26216973; PMCID: PMC4538663.
    Citations: 18     Fields:    Translation:HumansCells
  11. Ferrara MA, Di Caprio G, Managò S, De Angelis A, Sirleto L, Coppola G, De Luca AC. Label-free imaging and biochemical characterization of bovine sperm cells. Biosensors (Basel). 2015 Apr 01; 5(2):141-57. PMID: 25836358; PMCID: PMC4493542.
    Citations: 14     Fields:    Translation:AnimalsCells
  12. Schonbrun E, Di Caprio G. Differentiating neutrophils using the optical coulter counter. J Biomed Opt. 2015; 20(11):111205. PMID: 26187324.
    Citations:    Fields:    Translation:HumansCells
  13. Di Caprio G, Ferrara MA, Miccio L, Merola F, Memmolo P, Ferraro P, Coppola G. Holographic imaging of unlabelled sperm cells for semen analysis: a review. J Biophotonics. 2015 Oct; 8(10):779-89. PMID: 25491593.
    Citations: 14     Fields:    Translation:HumansAnimalsCells
  14. Schonbrun E, Möller G, Di Caprio G. Polarization encoded color camera. Opt Lett. 2014 Mar 15; 39(6):1433-6. PMID: 24690806.
    Citations:    Fields:    
  15. Schonbrun E, Malka R, Di Caprio G, Schaak D, Higgins JM. Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume. Cytometry A. 2014 Apr; 85(4):332-8. PMID: 24677669.
    Citations: 16     Fields:    Translation:HumansCells
  16. Di Caprio G, El Mallahi A, Ferraro P, Dale R, Coppola G, Dale B, Coppola G, Dubois F. 4D tracking of clinical seminal samples for quantitative characterization of motility parameters. Biomed Opt Express. 2014 Mar 01; 5(3):690-700. PMID: 24688806.
    Citations: 21     
  17. Merola F, Miccio L, Memmolo P, Di Caprio G, Galli A, Puglisi R, Balduzzi D, Coppola G, Netti P, Ferraro P. Digital holography as a method for 3D imaging and estimating the biovolume of motile cells. Lab Chip. 2013 Dec 07; 13(23):4512-6. PMID: 24129638.
    Citations: 29     Fields:    Translation:AnimalsCells
  18. Di Caprio G, Schaak D, Schonbrun E. Hyperspectral fluorescence microfluidic (HFM) microscopy. Biomed Opt Express. 2013; 4(8):1486-93. PMID: 24010010.
    Citations: 5     
  19. Schonbrun E, Di Caprio G, Schaak D. Dye exclusion microfluidic microscopy. Opt Express. 2013 Apr 08; 21(7):8793-8. PMID: 23571968.
    Citations: 8     Fields:    Translation:HumansAnimalsCells
  20. Coppola G, Di Caprio G, Wilding M, Ferraro P, Esposito G, Di Matteo L, Dale R, Coppola G, Dale B. Digital holographic microscopy for the evaluation of human sperm structure. Zygote. 2014 Nov; 22(4):446-54. PMID: 23469807.
    Citations: 7     Fields:    Translation:HumansCells
  21. Di Caprio G, Coppola G, De Stefano L, De Stefano M, Antonucci A, Congestri R, De Tommasi E. Shedding light on diatom photonics by means of digital holography. J Biophotonics. 2014 May; 7(5):341-50. PMID: 23242911.
    Citations: 11     Fields:    Translation:AnimalsCells
  22. Di Caprio G, Dardano P, Coppola G, Cabrini S, Mocella V. Digital holographic microscopy characterization of superdirective beam by metamaterial. Opt Lett. 2012 Apr 01; 37(7):1142-4. PMID: 22466175.
    Citations: 1     Fields:    
  23. Memmolo P, Di Caprio G, Distante C, Paturzo M, Puglisi R, Balduzzi D, Galli A, Coppola G, Ferraro P. Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy. Opt Express. 2011 Nov 07; 19(23):23215-26. PMID: 22109200.
    Citations: 12     Fields:    Translation:AnimalsCells
  24. Coppola G, Di Caprio G, Gioffré M, Puglisi R, Balduzzi D, Galli A, Miccio L, Paturzo M, Grilli S, Finizio A, Ferraro P. Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction. Opt Lett. 2010 Oct 15; 35(20):3390-2. PMID: 20967076.
    Citations: 4     Fields:    Translation:AnimalsCells
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.