Harvard Catalyst Profiles

Contact, publication, and social network information about Harvard faculty and fellows.

Giuseppe Di Caprio, Ph.D.

Title
Institution
Department
Address
Profile Picture

Biography
University of Naples Federico II, Napoli (Italy)MS07/2006Physics (cum laude)
University of Naples Federico II, Napoli (Italy)PhD12/2014Novel Technologies for Imaging (cum laude)
2013 - 2014
Best Paper award: “Hyperspectral Microscopy of Flowing Cells” - OSA Imaging and Applied Optics
2018 - 2027
National Academic Qualification as Associate Professor of Physics applied to Biology and Medicine

Overview
My research bridges Optical Microscopy, Biology and Image Processing. I am interested in mechanistic questions addressed at a molecular level aided by fluorescence microscopy imaging acquired with high temporal and spatial resolution. During the past years I focused on problems associated with viral particles endocytosis and fusion processes, in-vitro assembly of clathrin coated pits, the shape of intracellular energy gradients in living cells, Pyrin-mediated inflammasome assembly and activation, and the mechanisms that regulates cell internalization of Transferrin-decorated DNA origami barrels. Aspects of my studies involve close collaborations with other members in the Kirchhausen group and with external collaborators. Most of the imaging is carried using minimally invasive, 3D live-cell lattice light-sheet microscopy (LLSM); I have also been responsible for implementing the construction of one of these microscopes in the Kirchhausen lab as well as developing the computational processing tools used for the quantitative analysis of the data generated by myself and by other members of our group.

My second appointment is at the Massachusetts General Hospital, in collaboration with John Higgins, MD, and David Williams MD, where my research is dedicated to the development of material technologies for mimicking the environment of the circulatory system in vitro. This method provides biomarkers promising to improve management of sickle cell patients and to optimize the development and prioritization of candidate cures, and it’s currently used to validate the efficacy of BCL11A as therapeutic target in Sickle Cell Disease, Clinical Trials Identifier: NCT03282656 (Sponsor: David Williams – Boston Children’s Hospital).

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Di Caprio G, Schonbrun E, Gonçalves BP, Valdez JM, Wood DK, Higgins JM. High-throughput assessment of hemoglobin polymer in single red blood cells from sickle cell patients under controlled oxygen tension. Proc Natl Acad Sci U S A. 2019 12 10; 116(50):25236-25242. PMID: 31767751.
    Citations:    
  2. Schuler MH, Lewandowska A, Caprio GD, Skillern W, Upadhyayula S, Kirchhausen T, Shaw JM, Cunniff B. Miro1-mediated mitochondrial positioning shapes intracellular energy gradients required for cell migration. Mol Biol Cell. 2017 Aug 01; 28(16):2159-2169. PMID: 28615318.
    Citations: 5     Fields:    Translation:AnimalsCells
  3. Schonbrun E, Di Caprio G. A virtually imaged defocused array (VIDA) for high-speed 3D microscopy. J Biophotonics. 2016 10; 9(10):1044-1049. PMID: 26694084.
    Citations:    Fields:    Translation:HumansCells
  4. Di Caprio G, Stokes C, Higgins JM, Schonbrun E. Single-cell measurement of red blood cell oxygen affinity. Proc Natl Acad Sci U S A. 2015 Aug 11; 112(32):9984-9. PMID: 26216973.
    Citations: 3     Fields:    Translation:HumansCells
  5. Ferrara MA, Di Caprio G, Managò S, De Angelis A, Sirleto L, Coppola G, De Luca AC. Label-free imaging and biochemical characterization of bovine sperm cells. Biosensors (Basel). 2015 Apr 01; 5(2):141-57. PMID: 25836358.
    Citations: 2     Fields:    Translation:AnimalsCells
  6. Schonbrun E, Di Caprio G. Differentiating neutrophils using the optical coulter counter. J Biomed Opt. 2015; 20(11):111205. PMID: 26187324.
    Citations:    Fields:    Translation:HumansCells
  7. Di Caprio G, Ferrara MA, Miccio L, Merola F, Memmolo P, Ferraro P, Coppola G. Holographic imaging of unlabelled sperm cells for semen analysis: a review. J Biophotonics. 2015 Oct; 8(10):779-89. PMID: 25491593.
    Citations: 3     Fields:    Translation:HumansAnimalsCells
  8. Schonbrun E, Möller G, Di Caprio G. Polarization encoded color camera. Opt Lett. 2014 Mar 15; 39(6):1433-6. PMID: 24690806.
    Citations:    Fields:    
  9. Schonbrun E, Malka R, Di Caprio G, Schaak D, Higgins JM. Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume. Cytometry A. 2014 Apr; 85(4):332-8. PMID: 24677669.
    Citations: 9     Fields:    Translation:HumansCells
  10. Di Caprio G, El Mallahi A, Ferraro P, Dale R, Coppola G, Dale B, Coppola G, Dubois F. 4D tracking of clinical seminal samples for quantitative characterization of motility parameters. Biomed Opt Express. 2014 Mar 01; 5(3):690-700. PMID: 24688806.
    Citations: 10     
  11. Merola F, Miccio L, Memmolo P, Di Caprio G, Galli A, Puglisi R, Balduzzi D, Coppola G, Netti P, Ferraro P. Digital holography as a method for 3D imaging and estimating the biovolume of motile cells. Lab Chip. 2013 Dec 07; 13(23):4512-6. PMID: 24129638.
    Citations:    
  12. Di Caprio G, Schaak D, Schonbrun E. Hyperspectral fluorescence microfluidic (HFM) microscopy. Biomed Opt Express. 2013; 4(8):1486-93. PMID: 24010010.
    Citations: 2     
  13. Schonbrun E, Di Caprio G, Schaak D. Dye exclusion microfluidic microscopy. Opt Express. 2013 Apr 08; 21(7):8793-8. PMID: 23571968.
    Citations: 7     Fields:    Translation:HumansAnimalsCells
  14. Coppola G, Di Caprio G, Wilding M, Ferraro P, Esposito G, Di Matteo L, Dale R, Coppola G, Dale B. Digital holographic microscopy for the evaluation of human sperm structure. Zygote. 2014 Nov; 22(4):446-54. PMID: 23469807.
    Citations:    
  15. Di Caprio G, Coppola G, De Stefano L, De Stefano M, Antonucci A, Congestri R, De Tommasi E. Shedding light on diatom photonics by means of digital holography. J Biophotonics. 2014 May; 7(5):341-50. PMID: 23242911.
    Citations: 6     Fields:    Translation:AnimalsCells
  16. Di Caprio G, Dardano P, Coppola G, Cabrini S, Mocella V. Digital holographic microscopy characterization of superdirective beam by metamaterial. Opt Lett. 2012 Apr 01; 37(7):1142-4. PMID: 22466175.
    Citations: 1     Fields:    
  17. Memmolo P, Di Caprio G, Distante C, Paturzo M, Puglisi R, Balduzzi D, Galli A, Coppola G, Ferraro P. Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy. Opt Express. 2011 Nov 07; 19(23):23215-26. PMID: 22109200.
    Citations:    
  18. Coppola G, Di Caprio G, Gioffré M, Puglisi R, Balduzzi D, Galli A, Miccio L, Paturzo M, Grilli S, Finizio A, Ferraro P. Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction. Opt Lett. 2010 Oct 15; 35(20):3390-2. PMID: 20967076.
    Citations:    
Local representatives can answer questions about the Profiles website or help with editing a profile or issues with profile data. For assistance with this profile: HMS/HSDM faculty should contact feedbackcatalyst.harvard.edu. For faculty or fellow appointment updates and changes, please ask your appointing department to contact HMS. For fellow personal and demographic information, contact HMS Human Resources at human_resourceshms.harvard.edu. For faculty personal and demographic information, contact HMS Office for Faculty Affairs at facappthms.harvard.edu.
Di Caprio's Networks
Click the
Explore
buttons for more information and interactive visualizations!
Concepts (68)
Explore
_
Co-Authors (4)
Explore
_
Similar People (60)
Explore
_
Same Department 
Explore
_
Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.