Carlos Manlio Diaz Garcia, Ph.D.
Research Fellow in Neurobiology (INT)
Harvard Medical School
Harvard Medical School
Neurobiology WAB 328
220 Longwood Ave
Boston MA 02115
Postdoc awardee presentation and travel award. 13th Intl Conference on Brain Energy Metabolim
2017 - 2020
Ruth L. Kirschstein National Research Service Award (NRSA) individual postdoctoral fellowship (F32)
2016 - 2017
Fix Fund Postdoctoral Fellowship, Department of Neurobiology, Harvard Medical School
Ph.D. awarded with Honorific Mention, National Autonomous University of Mexico
International Travel Award, 57th Annual Meeting of the Biophysical Society
Travel Award, 1st Congress of the Federation of Neuroscience Societies from Latin-America
2010 - 2014
Ph.D. Scholarship, National Council of Science and Technology of Mexico CONACyT
Scholarship, Institute of Science and Technology of Mexico City ICyTDF
Scholarship, Network of Public MacroUniversities of Latin America and the Caribbean
Award for the Best Academic Performance, University of La Habana, Cuba
B.S. in Biochemistry awarded with High Honors, University of La Habana, Cuba
Carlos Manlio Diaz-Garcia is a postdoctoral researcher in the laboratory of Gary Yellen.
Brain metabolism must accommodate a wide dynamic range of energy demands from time to time and from cell type to cell type. Although the metabolic response to increased brain activity is the basis of well-known functional MRI signals, the nature of this metabolic response is still very controversial. It has been demonstrated that metabolism plays a key regulatory role in several neurological conditions, including epilepsy and neurodegenerative diseases. However, little is known about the mechanisms coupling neuronal excitability to metabolism, though the main metabolic pathways have been well established for decades now. The study of real-time dynamics of brain metabolism has been hampered by the insufficient spatial and temporal resolution of the methods, but such limitations can now be overcome by the use of genetically- encoded fluorescent biosensors. I use a combination of genetically-encoded fluorescent biosensors to determine glucose consumption as well as the NADH/NAD+ and ATP/ADP ratios. These sensors can give calibrated quantitative readouts within intact brain tissue, by using two-photon microscopy with either ratiometric or fluorescence lifetime imaging (FLIM). The Ca2+ indicator RCaMP1h and electrophysiologic recordings provide a readout of neuronal activity. Additionally, I measure the NAD(P)H and FAD+ autofluorescence in brain tissue as a proxy for the TCA cycle, and use Clark electrodes to measure the O2 level.
My current goal is to use these methods to study the energy metabolism of neurons and astrocytes in brain slices.
Aim 1 is to characterize the dynamics of glucose and NADH and ATP levels in the astrocytes and neurons (both glutamatergic and GABAergic) in hippocampal slices during resting and active states. This work will also explore the glycolytic contribution to the neuronal activity-induced acceleration of energy metabolism.
Aim 2 is to determine the mechanisms linking the neuronal activity to an increased metabolism, both the triggering stimuli (Na+, K+ and Ca2+ fluxes) and subsequent signaling (such as ionotropic or G-protein coupled receptors and protein kinases).
Aim 3 is to compare the mechanisms coupling neuronal activity to metabolism among neuronal somata, dendrites and axons/synaptic terminals.
This research project should provide definitive answers to these mechanistic questions, a necessary step towards understanding and treating the alterations that occur in disease states.
The research activities and funding listed below are automatically derived from
NIH ExPORTER and other sources, which might result in incorrect or missing items.
to make corrections and additions.
(DIAZ GARCIA, CARLOS MANLIO)
Apr 1, 2017 - Mar 31, 2020
Defining the cellular metabolic responses to brain activity using fluorescent biosensors
Role: Principal Investigator
Local representatives can answer questions about the Profiles website or help with editing a profile or issues with profile data. For assistance with this profile: HMS/HSDM faculty should contact feedbackcatalyst.harvard.edu. For faculty or fellow appointment updates and changes, please ask your appointing department to contact HMS. For fellow personal and demographic information, contact HMS Human Resources at human_resourceshms.harvard.edu. For faculty personal and demographic information, contact HMS Office for Faculty Affairs at facappthms.harvard.edu.