Mohamed Ismail Ashrafali, Ph.D.
Instructor in Ophthalmology
Massachusetts Eye and Ear Infirmary
Massachusetts Eye & Ear Infrm
Howe Lab, Rm 550
243 Charles St
Boston MA 02114
|Christian Medical College, Vellore, India||PhD||10/2013||Virology|
Our focus is to study the evolution of human adenoviruses associated with epidemic keratoconjunctivitis- a severe eye infection. We employ phylogenetics, recombination detection tools and in silico structural predictions for genetic characterization of novel adenoviruses. To study the differential viral gene regulation, I am mapping transcription factors occupancy across the genome by chromatin Immunoprecipitation and high throughput sequencing (ChIP-Seq). I am also studying adenovirus interactome by Affinity purification and Mass spectrometry methods. Studying viral DNA sensors and immune evasion strategies are my other current interests.
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04/2010 (IRB 7189)
(Ashrafli Mohamed Ismail)
Jun 1, 2010 - Jun 1, 2012
Fluid Research Grant, Christian Medical College, Vellore India
Development of LigAmp assay for sensitive detection of hepatitis B virus minor variants conferring resistance to antiviral drugs
Role Description: A virus population often exists as a complex mixture of genetic populations. Antiviral-resistant mutants could be circulating as minority variants in the mixed virus population that are not detected by standard sequencing methods. This study was intended to develop a technique called the ligation amplification assay (LigAmp) to identify minor drug-resistant variants of hepatitis B virus (HBV). In our validation, LigAmp assay showed potential clinical utility for appropriate monitoring of HBV therapy
Role: Principal Investigator
Dec 1, 2009 - Dec 1, 2012
Indian Council of Medical Research (ICMR)
Characterization of hepatitis B virus drug resistance in Indian subcontinent patients
Role Description: Hepatitis B virus (HBV) infection is a global public health problem. We aimed to characterize the predominant mutations affecting the antiviral action of major nucleot(s)ide analogue in chronic hepatitis B patients. We also characterized the prevailing HBV subtypes and genotypes and showed its association with treatment response. This study demonstrated several clinical variables and antiviral resistance mutations that could determine the therapeutic outcome of currently available anti-HBV drugs.
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