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Mohamed Ismail Ashrafali, Ph.D.

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Biography
Christian Medical College, Vellore, IndiaPhD10/2013Virology

Overview
Our focus is to study the evolution of human adenoviruses associated with epidemic keratoconjunctivitis- a severe eye infection. We employ phylogenetics, recombination detection tools and in silico structural predictions for genetic characterization of novel adenoviruses. To study the differential viral gene regulation, I am mapping transcription factors occupancy across the genome by chromatin Immunoprecipitation and high throughput sequencing (ChIP-Seq). I am also studying adenovirus interactome by Affinity purification and Mass spectrometry methods. Studying viral DNA sensors and immune evasion strategies are my other current interests.

Research
The research activities and funding listed below are automatically derived from NIH ExPORTER and other sources, which might result in incorrect or missing items. Faculty can login to make corrections and additions.
  1. 04/2010 (IRB 7189) (Ashrafli Mohamed Ismail) Jun 1, 2010 - Jun 1, 2012
    Fluid Research Grant, Christian Medical College, Vellore India
    Development of LigAmp assay for sensitive detection of hepatitis B virus minor variants conferring resistance to antiviral drugs
    Role Description: A virus population often exists as a complex mixture of genetic populations. Antiviral-resistant mutants could be circulating as minority variants in the mixed virus population that are not detected by standard sequencing methods. This study was intended to develop a technique called the ligation amplification assay (LigAmp) to identify minor drug-resistant variants of hepatitis B virus (HBV). In our validation, LigAmp assay showed potential clinical utility for appropriate monitoring of HBV therapy
    Role: Principal Investigator
  2. No. 5/8/7/7/2008-ECD-1 (Priya Abraham) Dec 1, 2009 - Dec 1, 2012
    Indian Council of Medical Research (ICMR)
    Characterization of hepatitis B virus drug resistance in Indian subcontinent patients
    Role Description: Hepatitis B virus (HBV) infection is a global public health problem. We aimed to characterize the predominant mutations affecting the antiviral action of major nucleot(s)ide analogue in chronic hepatitis B patients. We also characterized the prevailing HBV subtypes and genotypes and showed its association with treatment response. This study demonstrated several clinical variables and antiviral resistance mutations that could determine the therapeutic outcome of currently available anti-HBV drugs.
    Role: Co-Investigator

Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Ismail AM, Lee JS, Lee JY, Singh G, Dyer DW, Seto D, Chodosh J, Rajaiya J. Adenoviromics: Mining the Human Adenovirus Species D Genome. Front Microbiol. 2018; 9:2178. PMID: 30254627.
    Citations:    
  2. Lee JY, Lee JS, Materne EC, Rajala R, Ismail AM, Seto D, Dyer DW, Rajaiya J, Chodosh J. Bacterial RecA Protein Promotes Adenoviral Recombination during In Vitro Infection. mSphere. 2018 06 27; 3(3). PMID: 29925671.
    Citations:    Translation:HumansCells
  3. Ismail AM, Cui T, Dommaraju K, Singh G, Dehghan S, Seto J, Shrivastava S, Fedorova NB, Gupta N, Stockwell TB, Halpin R, Madupu R, Heim A, Kajon AE, Romanowski EG, Kowalski RP, Malathi J, Therese KL, Madhavan HN, Zhang Q, Ferreyra LJ, Jones MS, Rajaiya J, Dyer DW, Chodosh J, Seto D. Genomic analysis of a large set of currently-and historically-important human adenovirus pathogens. Emerg Microbes Infect. 2018 Feb 07; 7(1):10. PMID: 29410402.
    Citations: 1     Fields:    Translation:HumansCells
  4. Ismail AM, Lee JS, Dyer DW, Seto D, Rajaiya J, Chodosh J. Selection Pressure in the Human Adenovirus Fiber Knob Drives Cell Specificity in Epidemic Keratoconjunctivitis. J Virol. 2016 Nov 01; 90(21):9598-9607. PMID: 27512073.
    Citations: 1     Fields:    Translation:HumansCells
  5. Velay A, Jeulin H, Eschlimann M, Malvé B, Goehringer F, Bensenane M, Frippiat JP, Abraham P, Ismail AM, Murray JM, Combet C, Zoulim F, Bronowicki JP, Schvoerer E. Characterization of hepatitis B virus surface antigen variability and impact on HBs antigen clearance under nucleos(t)ide analogue therapy. J Viral Hepat. 2016 May; 23(5):387-98. PMID: 26742490.
    Citations: 5     Fields:    Translation:HumansAnimals
  6. Singh G, Zhou X, Lee JY, Yousuf MA, Ramke M, Ismail AM, Lee JS, Robinson CM, Seto D, Dyer DW, Jones MS, Rajaiya J, Chodosh J. Recombination of the epsilon determinant and corneal tropism: Human adenovirus species D types 15, 29, 56, and 69. Virology. 2015 Nov; 485:452-9. PMID: 26343864; PMCID: PMC4619159.
    Citations: 4     Fields:    Translation:HumansAnimalsCells
  7. Ismail AM, Raghavendran A, Sivakumar J, Radhakrishnan M, Rose W, Abraham P. Mother to child transmission of hepatitis B virus: a cause for concern. Indian J Med Microbiol. 2015 Feb; 33 Suppl:140-3. PMID: 25657134.
    Citations:    Fields:    Translation:HumansCells
  8. Ismail AM, Sachithanandham J, Eapen CE, Kannangai R, Abraham P. Performance of LigAmp assay for sensitive detection of drug-resistant hepatitis B virus minor variants in comparison with standard nucleotide sequencing. Mol Diagn Ther. 2014 Dec; 18(6):655-63. PMID: 25208639.
    Citations:    Fields:    Translation:HumansCells
  9. Ismail AM, Samuel P, Ramachandran J, Eapen CE, Kannangai R, Abraham P. Lamivudine monotherapy in chronic hepatitis B patients from the Indian subcontinent: antiviral resistance mutations and predictive factors of treatment response. Mol Diagn Ther. 2014 Feb; 18(1):63-71. PMID: 24030850.
    Citations: 3     Fields:    Translation:HumansCells
  10. Ismail AM, Puhazhenthi KS, Sivakumar J, Eapen CE, Kannangai R, Abraham P. Molecular epidemiology and genetic characterization of hepatitis B virus in the Indian subcontinent. Int J Infect Dis. 2014 Mar; 20:1-10. PMID: 24445226.
    Citations: 5     Fields:    Translation:HumansCells
  11. Ismail AM, Ramachandran J, Kannangai R, Abraham P. Antiviral efficacy of adefovir dipivoxil in the treatment of chronic hepatitis B subjects from Indian subcontinent. Indian J Med Microbiol. 2014 Jan-Mar; 32(1):60-3. PMID: 24399391.
    Citations: 2     Fields:    Translation:HumansCells
  12. Ramachandran J, Ismail AM, Chawla G, Fletcher GJ, Goel A, Eapen CE, Abraham P. Serum HBsAg quantification in treatment-naïve Indian patients with chronic hepatitis B. Indian J Gastroenterol. 2014 Mar; 33(2):131-5. PMID: 24052375.
    Citations: 3     Fields:    Translation:HumansCells
  13. Ismail AM, Goel A, Kannangai R, Abraham P. Further evidence of hepatitis B virus genotype I circulation in Northeast India. Infect Genet Evol. 2013 Aug; 18:60-5. PMID: 23665463.
    Citations: 1     Fields:    Translation:HumansCells
  14. Ismail AM, Sharma OP, Kumar MS, Eapen CE, Kannangai R, Abraham P. Virological response and antiviral resistance mutations in chronic hepatitis B subjects experiencing entecavir therapy: an Indian subcontinent perspective. Antiviral Res. 2013 May; 98(2):209-16. PMID: 23485939.
    Citations: 3     Fields:    Translation:HumansCellsCTClinical Trials
  15. Ismail AM, Sharma OP, Kumar MS, Kannangai R, Abraham P. Impact of rtI233V mutation in hepatitis B virus polymerase protein and adefovir efficacy: Homology modeling and molecular docking studies. Bioinformation. 2013; 9(3):121-5. PMID: 23423477; PMCID: PMC3569598.
    Citations: 2     
  16. Ismail AM, Devakumar S, Anantharam R, Fletcher GJ, Subramani T, John GT, Daniel D, Abraham P. Low frequency of occult hepatitis B infection in anti-HBc seropositive blood donors: experience from a tertiary care centre in South India. Blood Transfus. 2012 Apr; 10(2):230-2. PMID: 22153686; PMCID: PMC3320787.
    Citations: 7     Fields:    Translation:HumansCells
  17. Ismail AM, Sivakumar J, Anantharam R, Dayalan S, Samuel P, Fletcher GJ, Gnanamony M, Abraham P. Performance characteristics and comparison of Abbott and artus real-time systems for hepatitis B virus DNA quantification. J Clin Microbiol. 2011 Sep; 49(9):3215-21. PMID: 21795507; PMCID: PMC3165604.
    Citations: 7     Fields:    Translation:HumansCells
  18. Fletcher GJ, Samuel P, Christdas J, Gnanamony M, Ismail AM, Anantharam R, Eapen CE, Chacko MP, Daniel D, Kannangai R, Abraham P. Association of HLA and TNF polymorphisms with the outcome of HBV infection in the South Indian population. Genes Immun. 2011 Oct; 12(7):552-8. PMID: 21593777.
    Citations: 9     Fields:    Translation:Humans
  19. Ismail AM, Samuel P, Eapen CE, Kannangai R, Abraham P. Antiviral resistance mutations and genotype-associated amino acid substitutions in treatment-naïve hepatitis B virus-infected individuals from the Indian subcontinent. Intervirology. 2012; 55(1):36-44. PMID: 21311172.
    Citations: 10     Fields:    Translation:HumansCells
  20. Fletcher GJ, Gnanamony M, Samuel P, Ismail AM, Kannangai R, Daniel D, Eapen CE, Abraham P. Association of mannose-binding lectin polymorphisms and HBV outcome in a South Indian population. Int J Immunogenet. 2010 Jun; 37(3):177-84. PMID: 20193030.
    Citations: 1     Fields:    Translation:HumansCells
  21. Fletcher GJ, Gnanamony M, David J, Ismail AM, Subramani T, Abraham P. Do we need an 'in-house' neutralization assay for confirmation of hepatitis B surface antigen? Answers from a tertiary care hospital in India. J Gastroenterol Hepatol. 2010 May; 25(5):942-5. PMID: 19929924.
    Citations: 1     Fields:    Translation:HumansCells
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Funded by the NIH/NCATS Clinical and Translational Science Award (CTSA) program, grant number UL1TR001102, and through institutional support from Harvard University, Harvard Medical School, Harvard T.H. Chan School of Public Health, Beth Israel Deaconess Medical Center, Boston Children's Hospital, Brigham and Women's Hospital, Massachusetts General Hospital and the Dana Farber Cancer Institute.