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Daniel Irimia, Ph.D., M.D.

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Pioneers of Miniaturisation Prize

I am a bioengineer, trained as a physician, passionate about understanding the clinical consequences of neutrophil activities during disease. Neutrophils are the white blood cells that not only protect us against microbes, but in many instances, change their behavior and becomes detrimental. My research focuses on designing sophisticated tools to measure relevant neutrophil behaviors with the highest precision, directly from whole blood, towards better predictions, diagnostic, monitoring and treatment of in inflammation, infections and sepsis.

Recently, I found that neutrophils from patients after severe burn injuries lose their ability to pick up the shorter route through mazes towards chemical stimuli. Moreover, neutrophils from patients can sometimes move spontaneously though micron-sized channels, in the absence of directional signals. This neutrophil phenotype, never observed for healthy neutrophils, can predict, two days in advance, the occurrence of sepsis in patients after major burns. Further demonstrating the link between the defective neutrophil motility and sepsis, correcting neutrophil motility in animal models of burn injury could protect the animals from sepsis.

Overall, these observations indicate that neutrophils are key to monitoring and preventing sepsis. New tools are emerging to quantify the neutrophil phenotype in patients during inflammation, infections, and sepsis from just one droplet of blood.

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Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Otawara M, Roushan M, Wang X, Ellett F, Yu YM, Irimia D. Microfluidic Assay Measures Increased Neutrophil Extracellular Traps Circulating in Blood after Burn Injuries. Sci Rep. 2018 Nov 19; 8(1):16983. PMID: 30451882.
    View in: PubMed
  2. Raymond SL, Hawkins RB, Stortz JA, Murphy TJ, Ungaro R, Dirain ML, Nacionales DC, Hollen MK, Rincon JC, Larson SD, Brakenridge SC, Moore FA, Irimia D, Efron PA, Moldawer LL. Sepsis is associated with reduced spontaneous neutrophil migration velocity in human adults. PLoS One. 2018; 13(10):e0205327. PMID: 30300408.
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  3. Wang X, Jodoin E, Jorgensen J, Lee J, Markmann JJ, Cataltepe S, Irimia D. Progressive mechanical confinement of chemotactic neutrophils induces arrest, oscillations, and retrotaxis. J Leukoc Biol. 2018 Dec; 104(6):1253-1261. PMID: 30129679.
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  4. Muldur S, Marand AL, Ellett F, Irimia D. Measuring spontaneous neutrophil motility signatures from a drop of blood using microfluidics. Methods Cell Biol. 2018; 147:93-107. PMID: 30165965.
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  5. Ellett F, Jorgensen J, Marand AL, Liu YM, Martinez MM, Sein V, Butler KL, Lee J, Irimia D. Diagnosis of sepsis from a drop of blood by measurement of spontaneous neutrophil motility in a microfluidic assay. Nature Biomedical Engineering. 2018; 2(2):207–214.
  6. Krishnamoorthy N, Douda DN, Brüggemann TR, Ricklefs I, Duvall MG, Abdulnour RE, Martinod K, Tavares L, Wang X, Cernadas M, Israel E, Mauger DT, Bleecker ER, Castro M, Erzurum SC, Gaston BM, Jarjour NN, Wenzel S, Dunican E, Fahy JV, Irimia D, Wagner DD, Levy BD. Neutrophil cytoplasts induce TH17 differentiation and skew inflammation toward neutrophilia in severe asthma. Sci Immunol. 2018 Aug 03; 3(26). PMID: 30076281.
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  7. DeVos SL, Corjuc BT, Commins C, Dujardin S, Bannon RN, Corjuc D, Moore BD, Bennett RE, Jorfi M, Gonzales JA, Dooley PM, Roe AD, Pitstick R, Irimia D, Frosch MP, Carlson GA, Hyman BT. Tau reduction in the presence of amyloid-ß prevents tau pathology and neuronal death in vivo. Brain. 2018 Jul 01; 141(7):2194-2212. PMID: 29733334.
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  8. Kim JJ, Reátegui E, Hopke A, Jalali F, Roushan M, Doyle PS, Irimia D. Large-scale patterning of living colloids for dynamic studies of neutrophil-microbe interactions. Lab Chip. 2018 05 29; 18(11):1514-1520. PMID: 29770423.
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  9. Raymond SL, Hawkins RB, Murphy TJ, Rincon JC, Stortz JA, López MC, Ungaro R, Ellett F, Baker HV, Wynn JL, Moldawer LL, Irimia D, Larson SD. Impact of toll-like receptor 4 stimulation on human neonatal neutrophil spontaneous migration, transcriptomics, and cytokine production. J Mol Med (Berl). 2018 Jul; 96(7):673-684. PMID: 29808244.
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  10. Irimia D, Wang X. Inflammation-on-a-Chip: Probing the Immune System Ex Vivo. Trends Biotechnol. 2018 Sep; 36(9):923-937. PMID: 29728272.
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  11. Inoue Y, Liu YM, Otawara M, Chico Calero I, Stephanie Nam A, Yu YM, Chang P, Butler KL, Nazarian RM, Goverman J, Vakoc BJ, Irimia D. Resolvin D2 Limits Secondary Tissue Necrosis After Burn Wounds in Rats. J Burn Care Res. 2018 Apr 20; 39(3):423-432. PMID: 28877131.
    View in: PubMed
  12. Ellett F, Jorgensen J, Marand AL, Liu YM, Martinez MM, Sein V, Butler KL, Lee J, Irimia D. Diagnosis of sepsis from a drop of blood by measurement of spontaneous neutrophil motility in a microfluidic assay. Nat Biomed Eng. 2018 Apr; 2(4):207-214. PMID: 30283724.
    View in: PubMed
  13. Jorfi M, D'Avanzo C, Tanzi RE, Kim DY, Irimia D. Human Neurospheroid Arrays for In Vitro Studies of Alzheimer's Disease. Sci Rep. 2018 02 05; 8(1):2450. PMID: 29402979.
    View in: PubMed
  14. Boneschansker L, Jorgensen J, Ellett F, Briscoe DM, Irimia D. Convergent and Divergent Migratory Patterns of Human Neutrophils inside Microfluidic Mazes. Sci Rep. 2018 01 30; 8(1):1887. PMID: 29382882.
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  15. Wang X, Irimia D. Neutrophil Chemotaxis in One Droplet of Blood Using Microfluidic Assays. Methods Mol Biol. 2018; 1749:351-360. PMID: 29526009.
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  16. Ellett F, Irimia D. Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae. J Vis Exp. 2017 12 08; (130). PMID: 29286475.
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  17. Reátegui E, Jalali F, Khankhel AH, Wong E, Cho H, Lee J, Serhan CN, Dalli J, Elliott H, Irimia D. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering. 2017; 1(7):0094.
  18. Weng L, Ellett F, Edd J, Wong KHK, Uygun K, Irimia D, Stott SL, Toner M. A highly-occupied, single-cell trapping microarray for determination of cell membrane permeability. Lab Chip. 2017 11 21; 17(23):4077-4088. PMID: 29068447.
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  19. Raymond SL, Mathias BJ, Murphy TJ, Rincon JC, López MC, Ungaro R, Ellett F, Jorgensen J, Wynn JL, Baker HV, Moldawer LL, Irimia D, Larson SD. Neutrophil chemotaxis and transcriptomics in term and preterm neonates. Transl Res. 2017 12; 190:4-15. PMID: 28873345.
    View in: PubMed
  20. Jorfi M, D'Avanzo C, Kim DY, Irimia D. Three-Dimensional Models of the Human Brain Development and Diseases. Adv Healthc Mater. 2018 Jan; 7(1). PMID: 28845922.
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  21. Sandlin RD, Wong KHK, Boneschansker L, Carey TR, Miller KL, Rose G, Haber DA, Maheswaran S, Irimia D, Stott SL, Toner M. Preservative solution that stabilizes erythrocyte morphology and leukocyte viability under ambient conditions. Sci Rep. 2017 Jul 18; 7(1):5658. PMID: 28720788.
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  22. Reátegui E, Jalali F, Khankhel AH, Wong E, Cho H, Lee J, Serhan CN, Dalli J, Elliott H, Irimia D. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nat Biomed Eng. 2017; 1. PMID: 29057147.
    View in: PubMed
  23. Jalali F, Ellett F, Irimia D. Rapid antibiotic sensitivity testing in microwell arrays. Technology (Singap World Sci). 2017 Jun; 5(2):107-114. PMID: 28781994.
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  24. Dang I, Linkner J, Yan J, Irimia D, Faix J, Gautreau A. The Arp2/3 inhibitory protein Arpin is dispensable for chemotaxis. Biol Cell. 2017 Apr; 109(4):162-166. PMID: 28186323.
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  25. Ellett F, Irimia D. Microstructured Surface Arrays for Injection of Zebrafish Larvae. Zebrafish. 2017 04; 14(2):140-145. PMID: 28151697.
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  26. Ellett F, Jorgensen J, Frydman GH, Jones CN, Irimia D. Neutrophil Interactions Stimulate Evasive Hyphal Branching by Aspergillus fumigatus. PLoS Pathog. 2017 01; 13(1):e1006154. PMID: 28076396.
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  27. Chandrasekaran A, Ellett F, Jorgensen J, Irimia D. Temporal gradients limit the accumulation of neutrophils towards sources of chemoattractant. Microsyst Nanoeng. 2017; 3. PMID: 28713624.
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  28. Frydman GH, Le A, Ellett F, Jorgensen J, Fox JG, Tompkins RG, Irimia D. Technical Advance: Changes in neutrophil migration patterns upon contact with platelets in a microfluidic assay. J Leukoc Biol. 2017 03; 101(3):797-806. PMID: 27630219.
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  29. Kim JJ, Bong KW, Reátegui E, Irimia D, Doyle PS. Porous microwells for geometry-selective, large-scale microparticle arrays. Nat Mater. 2017 01; 16(1):139-146. PMID: 27595351.
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  30. Boneschansker L, Nakayama H, Eisenga M, Wedel J, Klagsbrun M, Irimia D, Briscoe DM. Netrin-1 Augments Chemokinesis in CD4+ T Cells In Vitro and Elicits a Proinflammatory Response In Vivo. J Immunol. 2016 08 15; 197(4):1389-98. PMID: 27430720; PMCID: PMC4976028 [Available on 08/15/17].
  31. Skoge M, Wong E, Hamza B, Bae A, Martel J, Kataria R, Keizer-Gunnink I, Kortholt A, Van Haastert PJ, Charras G, Janetopoulos C, Irimia D. A Worldwide Competition to Compare the Speed and Chemotactic Accuracy of Neutrophil-Like Cells. PLoS One. 2016; 11(6):e0154491. PMID: 27332963; PMCID: PMC4917115.
  32. Irimia D, Ellett F. Big insights from small volumes: deciphering complex leukocyte behaviors using microfluidics. J Leukoc Biol. 2016 08; 100(2):291-304. PMID: 27194799; PMCID: PMC4945356 [Available on 08/01/17].
  33. Inoue Y, Yu YM, Kurihara T, Vasilyev A, Ibrahim A, Oklu R, Zhao G, Nair AV, Brown D, Fischman AJ, Tompkins RG, Irimia D. Kidney and Liver Injuries After Major Burns in Rats Are Prevented by Resolvin D2. Crit Care Med. 2016 May; 44(5):e241-52. PMID: 26509319; PMCID: PMC4833510 [Available on 05/01/17].
  34. Norling LV, Headland SE, Dalli J, Arnardottir HH, Haworth O, Jones HR, Irimia D, Serhan CN, Perretti M. Proresolving and cartilage-protective actions of resolvin D1 in inflammatory arthritis. JCI Insight. 2016 Apr 21; 1(5):e85922. PMID: 27158677.
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  35. Wong KH, Sandlin RD, Carey TR, Miller KL, Shank AT, Oklu R, Maheswaran S, Haber DA, Irimia D, Stott SL, Toner M. The Role of Physical Stabilization in Whole Blood Preservation. Sci Rep. 2016 Feb 15; 6:21023. PMID: 26876805; PMCID: PMC4753451.
  36. Jones CN, Hoang AN, Martel JM, Dimisko L, Mikkola A, Inoue Y, Kuriyama N, Yamada M, Hamza B, Kaneki M, Warren HS, Brown DE, Irimia D. Microfluidic assay for precise measurements of mouse, rat, and human neutrophil chemotaxis in whole-blood droplets. J Leukoc Biol. 2016 07; 100(1):241-7. PMID: 26819316.
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  37. Boneschansker L, Inoue Y, Oklu R, Irimia D. Capillary plexuses are vulnerable to neutrophil extracellular traps. Integr Biol (Camb). 2016 Feb; 8(2):149-55. PMID: 26797289; PMCID: PMC4755882.
  38. Prentice-Mott HV, Meroz Y, Carlson A, Levine MA, Davidson MW, Irimia D, Charras GT, Mahadevan L, Shah JV. Directional memory arises from long-lived cytoskeletal asymmetries in polarized chemotactic cells. Proc Natl Acad Sci U S A. 2016 Feb 02; 113(5):1267-72. PMID: 26764383; PMCID: PMC4747767.
  39. Orr SK, Butler KL, Hayden D, Tompkins RG, Serhan CN, Irimia D. Gene Expression of Proresolving Lipid Mediator Pathways Is Associated With Clinical Outcomes in Trauma Patients. Crit Care Med. 2015 Dec; 43(12):2642-50. PMID: 26488221; PMCID: PMC4651815 [Available on 12/01/16].
  40. Bong KW, Kim JJ, Cho H, Lim E, Doyle PS, Irimia D. Synthesis of Cell-Adhesive Anisotropic Multifunctional Particles by Stop Flow Lithography and Streptavidin-Biotin Interactions. Langmuir. 2015 Dec 08; 31(48):13165-71. PMID: 26545155; PMCID: PMC4820324 [Available on 12/08/16].
  41. Jain NG, Wong EA, Aranyosi AJ, Boneschansker L, Markmann JF, Briscoe DM, Irimia D. Microfluidic mazes to characterize T-cell exploration patterns following activation in vitro. Integr Biol (Camb). 2015 Nov; 7(11):1423-31. PMID: 26325525; PMCID: PMC4630132.
  42. Cho H, Seo JH, Wong KH, Terasaki Y, Park J, Bong K, Arai K, Lo EH, Irimia D. Three-Dimensional Blood-Brain Barrier Model for in vitro Studies of Neurovascular Pathology. Sci Rep. 2015 Oct 27; 5:15222. PMID: 26503597; PMCID: PMC4622078.
  43. Takeda S, Wegmann S, Cho H, DeVos SL, Commins C, Roe AD, Nicholls SB, Carlson GA, Pitstick R, Nobuhara CK, Costantino I, Frosch MP, Müller DJ, Irimia D, Hyman BT. Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain. Nat Commun. 2015 Oct 13; 6:8490. PMID: 26458742; PMCID: PMC4608380.
  44. Jones CN, Dimisko L, Forrest K, Judice K, Poznansky MC, Markmann JF, Vyas JM, Irimia D. Human Neutrophils Are Primed by Chemoattractant Gradients for Blocking the Growth of Aspergillus fumigatus. J Infect Dis. 2016 Feb 01; 213(3):465-75. PMID: 26272935.
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  45. Hamza B, Irimia D. Whole blood human neutrophil trafficking in a microfluidic model of infection and inflammation. Lab Chip. 2015 Jun 21; 15(12):2625-33. PMID: 25987163; PMCID: PMC4457540.
  46. Bruneau S, Wedel J, Fakhouri F, Nakayama H, Boneschansker L, Irimia D, Daly KP, Briscoe DM. Translational implications of endothelial cell dysfunction in association with chronic allograft rejection. Pediatr Nephrol. 2016 Jan; 31(1):41-51. PMID: 25903640; PMCID: PMC4619184 [Available on 12/01/16].
  47. Hsu CH, Chen C, Irimia D, Toner M. Fast sorting of CD4+ T cells from whole blood using glass microbubbles. Technology (Singap World Sci). 2015 Mar; 3(1):38-44. PMID: 26161433.
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  48. Heo YS, Nagrath S, Moore AL, Zeinali M, Irimia D, Stott SL, Toth TL, Toner M. "Universal" vitrification of cells by ultra-fast cooling. Technology (Singap World Sci). 2015 Mar; 3(1):64-71. PMID: 25914896.
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  49. Aranyosi AJ, Wong EA, Irimia D. A neutrophil treadmill to decouple spatial and temporal signals during chemotaxis. Lab Chip. 2015 Jan 21; 15(2):549-556. PMID: 25412288; PMCID: PMC4268067.
  50. Jones CN, Moore M, Dimisko L, Alexander A, Ibrahim A, Hassell BA, Warren HS, Tompkins RG, Fagan SP, Irimia D. Spontaneous neutrophil migration patterns during sepsis after major burns. PLoS One. 2014; 9(12):e114509. PMID: 25489947.
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  51. Hori Y, Takeda S, Cho H, Wegmann S, Shoup TM, Takahashi K, Irimia D, Elmaleh DR, Hyman BT, Hudry E. A Food and Drug Administration-approved asthma therapeutic agent impacts amyloid ß in the brain in a transgenic model of Alzheimer disease. J Biol Chem. 2015 Jan 23; 290(4):1966-78. PMID: 25468905; PMCID: PMC4303653.
  52. Nery FC, da Hora CC, Yaqub U, Zhang X, McCarthy DM, Bhide PG, Irimia D, Breakefield XO. New methods for investigation of neuronal migration in embryonic brain explants. J Neurosci Methods. 2015 Jan 15; 239:80-4. PMID: 25291524; PMCID: PMC4268085.
  53. Boneschansker L, Yan J, Wong E, Briscoe DM, Irimia D. Microfluidic platform for the quantitative analysis of leukocyte migration signatures. Nat Commun. 2014 Sep 03; 5:4787. PMID: 25183261; PMCID: PMC4155519.
  54. Yan J, Irimia D. Stochastic variations of migration speed between cells in clonal populations. Technology (Singap World Sci). 2014 Sep; 2(3):185-188. PMID: 25436220.
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  55. Wong IY, Javaid S, Wong EA, Perk S, Haber DA, Toner M, Irimia D. Collective and individual migration following the epithelial-mesenchymal transition. Nat Mater. 2014 Nov; 13(11):1063-71. PMID: 25129619; PMCID: PMC4209198.
  56. Jones CN, Hoang AN, Dimisko L, Hamza B, Martel J, Irimia D. Microfluidic platform for measuring neutrophil chemotaxis from unprocessed whole blood. J Vis Exp. 2014 Jun 03; (88). PMID: 24962731; PMCID: PMC4186496.
  57. Nery FC, Atai NA, da Hora CC, Kim EY, Hettich J, Mempel TR, Breakefield XO, Irimia D. Microfluidic platform to evaluate migration of cells from patients with DYT1 dystonia. J Neurosci Methods. 2014 Jul 30; 232:181-188. PMID: 24880044.
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  58. Cho H, Hamza B, Wong EA, Irimia D. On-demand, competing gradient arrays for neutrophil chemotaxis. Lab Chip. 2014 Mar 07; 14(5):972-978. PMID: 24430002; PMCID: PMC3950309.
  59. Sung Y, Lue N, Hamza B, Martel J, Irimia D, Dasari RR, Choi W, Yaqoob Z, So P. Three-Dimensional Holographic Refractive-Index Measurement of Continuously Flowing Cells in a Microfluidic Channel. Phys Rev Appl. 2014 Feb 27; 1. PMID: 25419536.
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  60. Robertson AL, Holmes GR, Bojarczuk AN, Burgon J, Loynes CA, Chimen M, Sawtell AK, Hamza B, Willson J, Walmsley SR, Anderson SR, Coles MC, Farrow SN, Solari R, Jones S, Prince LR, Irimia D, Rainger GE, Kadirkamanathan V, Whyte MK, Renshaw SA. A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism. Sci Transl Med. 2014 Feb 26; 6(225):225ra29. PMID: 24574340; PMCID: PMC4247228.
  61. Hamza B, Wong E, Patel S, Cho H, Martel J, Irimia D. Retrotaxis of human neutrophils during mechanical confinement inside microfluidic channels. Integr Biol (Camb). 2014 Feb; 6(2):175-83. PMID: 24419464; PMCID: PMC3928968.
  62. Baik SH, Cha MY, Hyun YM, Cho H, Hamza B, Kim DK, Han SH, Choi H, Kim KH, Moon M, Lee J, Kim M, Irimia D, Mook-Jung I. Migration of neutrophils targeting amyloid plaques in Alzheimer's disease mouse model. Neurobiol Aging. 2014 Jun; 35(6):1286-92. PMID: 24485508; PMCID: PMC4248665.
  63. Irimia D. Cell migration in confined environments. Methods Cell Biol. 2014; 121:141-53. PMID: 24560508; PMCID: PMC4254565.
  64. Prentice-Mott HV, Chang CH, Mahadevan L, Mitchison TJ, Irimia D, Shah JV. Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments. Proc Natl Acad Sci U S A. 2013 Dec 24; 110(52):21006-11. PMID: 24324148; PMCID: PMC3876268.
  65. Hoang AN, Jones CN, Dimisko L, Hamza B, Martel J, Kojic N, Irimia D. Measuring neutrophil speed and directionality during chemotaxis, directly from a droplet of whole blood. Technology (Singap World Sci). 2013 Oct 02; 1(1):49. PMID: 24809064.
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  66. Mantel PY, Hoang AN, Goldowitz I, Potashnikova D, Hamza B, Vorobjev I, Ghiran I, Toner M, Irimia D, Ivanov AR, Barteneva N, Marti M. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe. 2013 May 15; 13(5):521-534. PMID: 23684304.
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  67. Desai SP, Bhatia SN, Toner M, Irimia D. Mitochondrial localization and the persistent migration of epithelial cancer cells. Biophys J. 2013 May 07; 104(9):2077-88. PMID: 23663851; PMCID: PMC3647149.
  68. Kurihara T, Jones CN, Yu YM, Fischman AJ, Watada S, Tompkins RG, Fagan SP, Irimia D. Resolvin D2 restores neutrophil directionality and improves survival after burns. FASEB J. 2013 Jun; 27(6):2270-81. PMID: 23430978; PMCID: PMC3659356.
  69. Cho H, Hashimoto T, Wong E, Hori Y, Wood LB, Zhao L, Haigis KM, Hyman BT, Irimia D. Microfluidic chemotaxis platform for differentiating the roles of soluble and bound amyloid-ß on microglial accumulation. Sci Rep. 2013; 3:1823. PMID: 23665843; PMCID: PMC3650586.
  70. Jones CN, Dalli J, Dimisko L, Wong E, Serhan CN, Irimia D. Microfluidic chambers for monitoring leukocyte trafficking and humanized nano-proresolving medicines interactions. Proc Natl Acad Sci U S A. 2012 Dec 11; 109(50):20560-5. PMID: 23185003; PMCID: PMC3528552.
  71. Bohr S, Patel SJ, Sarin D, Irimia D, Yarmush ML, Berthiaume F. Resolvin D2 prevents secondary thrombosis and necrosis in a mouse burn wound model. Wound Repair Regen. 2013 Jan-Feb; 21(1):35-43. PMID: 23110665; PMCID: PMC3746505.
  72. El-Haibi CP, Bell GW, Zhang J, Collmann AY, Wood D, Scherber CM, Csizmadia E, Mariani O, Zhu C, Campagne A, Toner M, Bhatia SN, Irimia D, Vincent-Salomon A, Karnoub AE. Critical role for lysyl oxidase in mesenchymal stem cell-driven breast cancer malignancy. Proc Natl Acad Sci U S A. 2012 Oct 23; 109(43):17460-5. PMID: 23033492; PMCID: PMC3491529.
  73. Xu C, Poh YK, Roes I, O'Cearbhaill ED, Matthiesen ME, Mu L, Yang SY, Miranda-Nieves D, Irimia D, Karp JM. A portable chemotaxis platform for short and long term analysis. PLoS One. 2012; 7(9):e44995. PMID: 23028729; PMCID: PMC3444504.
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  75. Ritch JJ, Valencia A, Alexander J, Sapp E, Gatune L, Sangrey GR, Sinha S, Scherber CM, Zeitlin S, Sadri-Vakili G, Irimia D, Difiglia M, Kegel KB. Multiple phenotypes in Huntington disease mouse neural stem cells. Mol Cell Neurosci. 2012 May; 50(1):70-81. PMID: 22508027; PMCID: PMC3383872.
  76. Oh SF, Dona M, Fredman G, Krishnamoorthy S, Irimia D, Serhan CN. Resolvin E2 formation and impact in inflammation resolution. J Immunol. 2012 May 01; 188(9):4527-34. PMID: 22450811; PMCID: PMC3331964.
  77. Scherber C, Aranyosi AJ, Kulemann B, Thayer SP, Toner M, Iliopoulos O, Irimia D. Epithelial cell guidance by self-generated EGF gradients. Integr Biol (Camb). 2012 Mar; 4(3):259-69. PMID: 22314635; PMCID: PMC3440622.
  78. Aldridge BB, Fernandez-Suarez M, Heller D, Ambravaneswaran V, Irimia D, Toner M, Fortune SM. Asymmetry and aging of mycobacterial cells lead to variable growth and antibiotic susceptibility. Science. 2012 Jan 06; 335(6064):100-4. PMID: 22174129; PMCID: PMC3397429.
  79. Watkins N, Irimia D, Toner M, Bashir R. On a chip. IEEE Pulse. 2011 Nov; 2(6):19-27. PMID: 22147065; PMCID: PMC4493052.
  80. Heo YS, Lee HJ, Hassell BA, Irimia D, Toth TL, Elmoazzen H, Toner M. Controlled loading of cryoprotectants (CPAs) to oocyte with linear and complex CPA profiles on a microfluidic platform. Lab Chip. 2011 Oct 21; 11(20):3530-7. PMID: 21887438; PMCID: PMC3755277.
  81. Yang R, Fredman G, Krishnamoorthy S, Agrawal N, Irimia D, Piomelli D, Serhan CN. Decoding functional metabolomics with docosahexaenoyl ethanolamide (DHEA) identifies novel bioactive signals. J Biol Chem. 2011 Sep 09; 286(36):31532-41. PMID: 21757729; PMCID: PMC3173121.
  82. Hoerning A, Koss K, Datta D, Boneschansker L, Jones CN, Wong IY, Irimia D, Calzadilla K, Benitez F, Hoyer PF, Harmon WE, Briscoe DM. Subsets of human CD4(+) regulatory T cells express the peripheral homing receptor CXCR3. Eur J Immunol. 2011 Aug; 41(8):2291-302. PMID: 21538345; PMCID: PMC3383044.
  83. Rosenbach AE, Koria P, Goverman J, Kotz KT, Gupta A, Yu M, Fagan SP, Irimia D, Tompkins RG. Microfluidics for T- lymphocyte cell separation and inflammation monitoring in burn patients. Clin Transl Sci. 2011 Feb; 4(1):63-8. PMID: 21348958; PMCID: PMC3057059.
  84. Konry T, Smolina I, Yarmush JM, Irimia D, Yarmush ML. Ultrasensitive detection of low-abundance surface-marker protein using isothermal rolling circle amplification in a microfluidic nanoliter platform. Small. 2011 Feb 07; 7(3):395-400. PMID: 21294269; PMCID: PMC3110589.
  85. Smolen GA, Zhang J, Zubrowski MJ, Edelman EJ, Luo B, Yu M, Ng LW, Scherber CM, Schott BJ, Ramaswamy S, Irimia D, Root DE, Haber DA. A genome-wide RNAi screen identifies multiple RSK-dependent regulators of cell migration. Genes Dev. 2010 Dec 01; 24(23):2654-65. PMID: 21062900; PMCID: PMC2994039.
  86. Kane BJ, Younan G, Helm D, Dastouri P, Prentice-Mott H, Irimia D, Chan RK, Toner M, Orgill DP. Controlled induction of distributed microdeformation in wounded tissue via a microchamber array dressing. J Biomed Mater Res A. 2010 Nov; 95(2):333-40. PMID: 20607869; PMCID: PMC2943997.
  87. Prentice-Mott H, Toner M, Irimia D. Microfluidic proportional flow controller. J Micromech Microeng. 2010 Oct 15; 20(11):1-8. PMID: 21874096.
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  88. Stott SL, Hsu CH, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, Floyd FP, Gilman AJ, Lord JB, Winokur D, Springer S, Irimia D, Nagrath S, Sequist LV, Lee RJ, Isselbacher KJ, Maheswaran S, Haber DA, Toner M. Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proc Natl Acad Sci U S A. 2010 Oct 26; 107(43):18392-7. PMID: 20930119; PMCID: PMC2972993.
  89. Kotz KT, Xiao W, Miller-Graziano C, Qian WJ, Russom A, Warner EA, Moldawer LL, De A, Bankey PE, Petritis BO, Camp DG, Rosenbach AE, Goverman J, Fagan SP, Brownstein BH, Irimia D, Xu W, Wilhelmy J, Mindrinos MN, Smith RD, Davis RW, Tompkins RG, Toner M. Clinical microfluidics for neutrophil genomics and proteomics. Nat Med. 2010 Sep; 16(9):1042-7. PMID: 20802500; PMCID: PMC3136804.
  90. Irimia D. Microfluidic technologies for temporal perturbations of chemotaxis. Annu Rev Biomed Eng. 2010 Aug 15; 12:259-84. PMID: 20450351; PMCID: PMC3153854.
  91. Ambravaneswaran V, Wong IY, Aranyosi AJ, Toner M, Irimia D. Directional decisions during neutrophil chemotaxis inside bifurcating channels. Integr Biol (Camb). 2010 Nov; 2(11-12):639-47. PMID: 20676444; PMCID: PMC3001269.
  92. Butler KL, Ambravaneswaran V, Agrawal N, Bilodeau M, Toner M, Tompkins RG, Fagan S, Irimia D. Burn injury reduces neutrophil directional migration speed in microfluidic devices. PLoS One. 2010 Jul 30; 5(7):e11921. PMID: 20689600; PMCID: PMC2912851.
  93. Wolfer A, Wittner BS, Irimia D, Flavin RJ, Lupien M, Gunawardane RN, Meyer CA, Lightcap ES, Tamayo P, Mesirov JP, Liu XS, Shioda T, Toner M, Loda M, Brown M, Brugge JS, Ramaswamy S. MYC regulation of a "poor-prognosis" metastatic cancer cell state. Proc Natl Acad Sci U S A. 2010 Feb 23; 107(8):3698-703. PMID: 20133671; PMCID: PMC2840447.
  94. Chen C, Skog J, Hsu CH, Lessard RT, Balaj L, Wurdinger T, Carter BS, Breakefield XO, Toner M, Irimia D. Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip. 2010 Feb 21; 10(4):505-11. PMID: 20126692; PMCID: PMC3136803.
  95. Irimia D. Cutting edge: electronic counting of white blood cells. Lab Chip. 2009 Oct 21; 9(20):2875-6. PMID: 19789737; PMCID: PMC4482756.
  96. Stoothoff W, Jones PB, Spires-Jones TL, Joyner D, Chhabra E, Bercury K, Fan Z, Xie H, Bacskai B, Edd J, Irimia D, Hyman BT. Differential effect of three-repeat and four-repeat tau on mitochondrial axonal transport. J Neurochem. 2009 Oct; 111(2):417-27. PMID: 19686388; PMCID: PMC2831412.
  97. Irimia D, Toner M. Spontaneous migration of cancer cells under conditions of mechanical confinement. Integr Biol (Camb). 2009 Sep; 1(8-9):506-12. PMID: 20023765; PMCID: PMC3763902.
  98. Russom A, Irimia D, Toner M. Chemical gradient-mediated melting curve analysis for genotyping of SNPs. Electrophoresis. 2009 Jul; 30(14):2536-43. PMID: 19593749; PMCID: PMC2784656.
  99. Irimia D, Balázsi G, Agrawal N, Toner M. Adaptive-control model for neutrophil orientation in the direction of chemical gradients. Biophys J. 2009 May 20; 96(10):3897-916. PMID: 19450463; PMCID: PMC2712156.
  100. Edd JF, Humphry KJ, Irimia D, Weitz DA, Toner M. Nucleation and solidification in static arrays of monodisperse drops. Lab Chip. 2009 Jul 07; 9(13):1859-65. PMID: 19532960; PMCID: PMC4476639.
  101. Kasuga K, Yang R, Porter TF, Agrawal N, Petasis NA, Irimia D, Toner M, Serhan CN. Rapid appearance of resolvin precursors in inflammatory exudates: novel mechanisms in resolution. J Immunol. 2008 Dec 15; 181(12):8677-87. PMID: 19050288; PMCID: PMC2664686.
  102. Irimia D, Mindrinos M, Russom A, Xiao W, Wilhelmy J, Wang S, Heath JD, Kurn N, Tompkins RG, Davis RW, Toner M. Genome-wide transcriptome analysis of 150 cell samples. Integr Biol (Camb). 2009 Jan; 1(1):99-107. PMID: 20023796; PMCID: PMC3763906.
  103. Agrawal N, Toner M, Irimia D. Neutrophil migration assay from a drop of blood. Lab Chip. 2008 Dec; 8(12):2054-61. PMID: 19023468; PMCID: PMC3770894.
  104. Hsu CH, Di Carlo D, Chen C, Irimia D, Toner M. Microvortex for focusing, guiding and sorting of particles. Lab Chip. 2008 Dec; 8(12):2128-34. PMID: 19023476; PMCID: PMC4142319.
  105. Nery FC, Zeng J, Niland BP, Hewett J, Farley J, Irimia D, Li Y, Wiche G, Sonnenberg A, Breakefield XO. TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton. J Cell Sci. 2008 Oct 15; 121(Pt 20):3476-86. PMID: 18827015; PMCID: PMC3539201.
  106. Prabhakarpandian B, Pant K, Scott RC, Pattillo CB, Patillo CB, Irimia D, Kiani MF, Sundaram S. Synthetic microvascular networks for quantitative analysis of particle adhesion. Biomed Microdevices. 2008 Aug; 10(4):585-95. PMID: 18327641; PMCID: PMC4476637.
  107. Maheswaran S, Sequist LV, Nagrath S, Ulkus L, Brannigan B, Collura CV, Inserra E, Diederichs S, Iafrate AJ, Bell DW, Digumarthy S, Muzikansky A, Irimia D, Settleman J, Tompkins RG, Lynch TJ, Toner M, Haber DA. Detection of mutations in EGFR in circulating lung-cancer cells. N Engl J Med. 2008 Jul 24; 359(4):366-77. PMID: 18596266; PMCID: PMC3551471.
  108. Edd JF, Di Carlo D, Humphry KJ, Köster S, Irimia D, Weitz DA, Toner M. Controlled encapsulation of single-cells into monodisperse picolitre drops. Lab Chip. 2008 Aug; 8(8):1262-4. PMID: 18651066; PMCID: PMC2570196.
  109. Park K, Jang J, Irimia D, Sturgis J, Lee J, Robinson JP, Toner M, Bashir R. 'Living cantilever arrays' for characterization of mass of single live cells in fluids. Lab Chip. 2008 Jul; 8(7):1034-41. PMID: 18584076; PMCID: PMC3804646.
  110. Kelly RT, Tang K, Irimia D, Toner M, Smith RD. Elastomeric microchip electrospray emitter for stable cone-jet mode operation in the nanoflow regime. Anal Chem. 2008 May 15; 80(10):3824-31. PMID: 18419138; PMCID: PMC2692495.
  111. Parekkadan B, Berdichevsky Y, Irimia D, Leeder A, Yarmush G, Toner M, Levine JB, Yarmush ML. Cell-cell interaction modulates neuroectodermal specification of embryonic stem cells. Neurosci Lett. 2008 Jun 20; 438(2):190-5. PMID: 18467031; PMCID: PMC2448393.
  112. Russom A, Sethu P, Irimia D, Mindrinos MN, Calvano SE, Garcia I, Finnerty C, Tannahill C, Abouhamze A, Wilhelmy J, López MC, Baker HV, Herndon DN, Lowry SF, Maier RV, Davis RW, Moldawer LL, Tompkins RG, Toner M. Microfluidic leukocyte isolation for gene expression analysis in critically ill hospitalized patients. Clin Chem. 2008 May; 54(5):891-900. PMID: 18375483; PMCID: PMC4011019.
  113. Di Carlo D, Edd JF, Irimia D, Tompkins RG, Toner M. Equilibrium separation and filtration of particles using differential inertial focusing. Anal Chem. 2008 Mar 15; 80(6):2204-11. PMID: 18275222.
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  114. Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M. Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 2007 Dec 20; 450(7173):1235-9. PMID: 18097410; PMCID: PMC3090667.
  115. Di Carlo D, Irimia D, Tompkins RG, Toner M. Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci U S A. 2007 Nov 27; 104(48):18892-7. PMID: 18025477; PMCID: PMC2141878.
  116. Vu HN, Li Y, Casali M, Irimia D, Megeed Z, Yarmush ML. A microfluidic bioreactor for increased active retrovirus output. Lab Chip. 2008 Jan; 8(1):75-80. PMID: 18094764.
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  117. Irimia D, Charras G, Agrawal N, Mitchison T, Toner M. Polar stimulation and constrained cell migration in microfluidic channels. Lab Chip. 2007 Dec; 7(12):1783-90. PMID: 18030401; PMCID: PMC3001245.
  118. Cheng X, Irimia D, Dixon M, Ziperstein JC, Demirci U, Zamir L, Tompkins RG, Toner M, Rodriguez WR. A microchip approach for practical label-free CD4+ T-cell counting of HIV-infected subjects in resource-poor settings. J Acquir Immune Defic Syndr. 2007 Jul 01; 45(3):257-61. PMID: 17414933.
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  119. Cheng X, Liu YS, Irimia D, Demirci U, Yang L, Zamir L, Rodríguez WR, Toner M, Bashir R. Cell detection and counting through cell lysate impedance spectroscopy in microfluidic devices. Lab Chip. 2007 Jun; 7(6):746-55. PMID: 17538717; PMCID: PMC4476634.
  120. Daniel Irimia. Pneumatic Valves in Encyclopedia of Micro- and Nanofluidics edited by Li, Dongqing. 2006.
  121. Daniel Irimia. Techniques of Manipulating Cells in Encyclopedia of Micro- and Nanofluidics edited by Li, Dongqing. 2006.
  122. Cheng X, Irimia D, Dixon M, Sekine K, Demirci U, Zamir L, Tompkins RG, Rodriguez W, Toner M. A microfluidic device for practical label-free CD4(+) T cell counting of HIV-infected subjects. Lab Chip. 2007 Feb; 7(2):170-8. PMID: 17268618; PMCID: PMC4028372.
  123. King KR, Wang S, Irimia D, Jayaraman A, Toner M, Yarmush ML. A high-throughput microfluidic real-time gene expression living cell array. Lab Chip. 2007 Jan; 7(1):77-85. PMID: 17180208; PMCID: PMC3205973.
  124. Irimia D, Geba DA, Toner M. Universal microfluidic gradient generator. Anal Chem. 2006 May 15; 78(10):3472-7. PMID: 16689552; PMCID: PMC3770893.
  125. Vianello F, Papeta N, Chen T, Kraft P, White N, Hart WK, Kircher MF, Swart E, Rhee S, Palù G, Irimia D, Toner M, Weissleder R, Poznansky MC. Murine B16 melanomas expressing high levels of the chemokine stromal-derived factor-1/CXCL12 induce tumor-specific T cell chemorepulsion and escape from immune control. J Immunol. 2006 Mar 01; 176(5):2902-14. PMID: 16493048.
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  126. Irimia D, Toner M. Cell handling using microstructured membranes. Lab Chip. 2006 Mar; 6(3):345-52. PMID: 16511616; PMCID: PMC3769100.
  127. Daniel Irimia. Capillary Force Valves in Encyclopedia of Micro- and Nanofluidics edited by Li, Dongqing. 2006.
  128. Aksan A, Irimia D, He X, Toner M. Desiccation kinetics of biopreservation solutions in microchannels. J Appl Phys. 2006; 99(6):2181280. PMID: 24155501.
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  129. Irimia D, Liu SY, Tharp WG, Samadani A, Toner M, Poznansky MC. Microfluidic system for measuring neutrophil migratory responses to fast switches of chemical gradients. Lab Chip. 2006 Feb; 6(2):191-8. PMID: 16450027; PMCID: PMC3763904.
  130. Tharp WG, Yadav R, Irimia D, Upadhyaya A, Samadani A, Hurtado O, Liu SY, Munisamy S, Brainard DM, Mahon MJ, Nourshargh S, van Oudenaarden A, Toner MG, Poznansky MC. Neutrophil chemorepulsion in defined interleukin-8 gradients in vitro and in vivo. J Leukoc Biol. 2006 Mar; 79(3):539-54. PMID: 16365152.
    View in: PubMed
  131. Toner M, Irimia D. Blood-on-a-chip. Annu Rev Biomed Eng. 2005; 7:77-103. PMID: 16004567; PMCID: PMC3779643.
  132. Irimia D, Tompkins RG, Toner M. Single-cell chemical lysis in picoliter-scale closed volumes using a microfabricated device. Anal Chem. 2004 Oct 15; 76(20):6137-43. PMID: 15481964.
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  133. Irimia D, Karlsson JO. Kinetics of intracellular ice formation in one-dimensional arrays of interacting biological cells. Biophys J. 2005 Jan; 88(1):647-60. PMID: 15475590; PMCID: PMC1305042.
  134. Stott SL, Irimia D, Karlsson JO. Parametric analysis of intercellular ice propagation during cryosurgery, simulated using monte carlo techniques. Technol Cancer Res Treat. 2004 Apr; 3(2):113-23. PMID: 15059017.
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  135. Irimia, D., Karlsson, J.O.M. Development of a Cell Patterning Technique Using Poly(Ethylene Glycol) Disilane. Journal of Biomedical Microdevices. 2003; 5(3):185-194.
  136. Irimia D, Karlsson JO. Kinetics and mechanism of intercellular ice propagation in a micropatterned tissue construct. Biophys J. 2002 Apr; 82(4):1858-68. PMID: 11916845; PMCID: PMC1301983.
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Funded by the NIH/NCATS Clinical and Translational Science Award (CTSA) program, grant number UL1TR001102, and through institutional support from Harvard University, Harvard Medical School, Harvard T.H. Chan School of Public Health, Beth Israel Deaconess Medical Center, Boston Children's Hospital, Brigham and Women's Hospital, Massachusetts General Hospital and the Dana Farber Cancer Institute.