Harvard Catalyst Profiles

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Emmanouil Rousakis, Ph.D.

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Overview
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I am a synthetic organic chemist, with expertise in the design and synthesis of luminescent imaging agents for quantifying physiological analytes and an interest in developing medical diagnostics based on optical detection. I am motivated by the ongoing need for portable, easy-to-use diagnostic technologies for monitoring tissue conditions in both pre-hospital environments and in-hospital care.

I am passionate about my work, and I enjoy working in an application-oriented environment where teams of clinicians and scientists with different backgrounds interact to generate ideas, advance them into discovery projects and solve complex problems that may arise in the process. In addition, I am excited at the prospect of translating research projects into tools or technologies that can have an impact in improving healthcare.

Bibliographic
selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
  1. Cascales JP, Li X, Roussakis E, Evans CL. A Patient-Ready Wearable Transcutaneous CO2 Sensor. Biosensors (Basel). 2022 May 13; 12(5). PMID: 35624634; PMCID: PMC9138690.
    Citations:    Fields:    Translation:Humans
  2. Marks HL, Cook K, Roussakis E, Cascales JP, Korunes-Miller JT, Grinstaff MW, Evans CL. Quantitative Luminescence Photography of a Swellable Hydrogel Dressing with a Traffic-Light Response to Oxygen. Adv Healthc Mater. 2022 05; 11(10):e2101605. PMID: 35120400.
    Citations:    Fields:    
  3. Li X, Roussakis E, Cascales JP, Marks HL, Witthauer L, Evers M, Manstein D, Evans CL . Optimization of bright, highly flexible, and humidity insensitive porphyrin-based oxygen-sensing materials. Journal of Materials Chemistry C. 2021; 9(24):7555-7567. View Publication.
  4. Cascales JP, Greenfield DA, Roussakis E, Witthauer L, Li X, Goss A, Evans CL . Wireless wearable sensor paired with machine learning for the quantification of tissue oxygenation. IEEE Internet of Things Journal. 2021; 8(24):17557 - 17567. View Publication.
  5. Peng F, Jeong S, Gonzalez G, Marks H, Ho A, Roussakis E, Krauledat PB, Hansen P, Evans CL. Assessment of Glial Fibrillary Acidic Protein Binding to the Surface of Leukocytes with Dark-Field Imaging and Computational Analysis. Advanced Functional Materials. 2021; 31(11):2009229. View Publication.
  6. Marks H, Bucknor A, Roussakis E, Nowell N, Kamali P, Cascales JP, Kazei D, Lin SJ, Evans CL. A paintable phosphorescent bandage for postoperative tissue oxygen assessment in DIEP flap reconstruction. Sci Adv. 2020 12; 6(51). PMID: 33355131.
    Citations: 2     Fields:    
  7. Witthauer L, Cascales JP, Roussakis E, Li X, Goss A, Chen Y, Evans CL. Portable Oxygen-Sensing Device for the Improved Assessment of Compartment Syndrome and other Hypoxia-Related Conditions. ACS Sens. 2021 01 22; 6(1):43-53. PMID: 33325684.
    Citations: 1     Fields:    Translation:Animals
  8. Cascales JP, Roussakis E, Witthauer L, Goss A, Li X, Chen Y, Marks HL, Evans CL. Wearable device for remote monitoring of transcutaneous tissue oxygenation. Biomed Opt Express. 2020 Dec 01; 11(12):6989-7002. PMID: 33408975.
    Citations: 2     
  9. Roussakis E, Cascales JP, Marks HL, Li X, Grinstaff M, Evans CL. Humidity-Insensitive Tissue Oxygen Tension Sensing for Wearable Devices†. Photochem Photobiol. 2020 03; 96(2):373-379. PMID: 31869435.
    Citations: 2     Fields:    Translation:Humans
  10. Roussakis E, Ortines RV, Pinsker BL, Mooers CT, Evans CL, Miller LS, Calderón-Colón X. Theranostic biocomposite scaffold membrane. Biomaterials. 2019 08; 212:17-27. PMID: 31100480.
    Citations: 3     Fields:    Translation:Animals
  11. Gkikas M, Peponis T, Mesar T, Hong C, Avery RK, Roussakis E, Yoo HJ, Parakh A, Patino M, Sahani DV, Watkins MT, Oklu R, Evans CL, Albadawi H, Velmahos G, Olsen BD. Systemically Administered Hemostatic Nanoparticles for Identification and Treatment of Internal Bleeding. ACS Biomater Sci Eng. 2019 May 13; 5(5):2563-2576. PMID: 33405762.
    Citations: 3     Fields:    
  12. Koolen PGL, Li Z, Roussakis E, Paul MA, Ibrahim AMS, Matyal R, Huang T, Evans CL, Lin SJ. Oxygen-Sensing Paint-On Bandage: Calibration of a Novel Approach in Tissue Perfusion Assessment. Plast Reconstr Surg. 2017 Jul; 140(1):89-96. PMID: 28654595.
    Citations: 4     Fields:    Translation:Animals
  13. Sakadžic S, Yaseen MA, Jaswal R, Roussakis E, Dale AM, Buxton RB, Vinogradov SA, Boas DA, Devor A. Two-photon microscopy measurement of cerebral metabolic rate of oxygen using periarteriolar oxygen concentration gradients. Neurophotonics. 2016 Oct; 3(4):045005. PMID: 27774493; PMCID: PMC5066455.
    Citations: 22     
  14. Roussakis E, Li Z, Nowell NH, Nichols AJ, Evans CL. Bright, "Clickable" Porphyrins for the Visualization of Oxygenation under Ambient Light. Angew Chem Int Ed Engl. 2015 Dec 01; 54(49):14728-31. PMID: 26510549; PMCID: PMC4715754.
    Citations: 9     Fields:    
  15. Roussakis E, Li Z, Nichols AJ, Evans CL. Oxygen-Sensing Methods in Biomedicine from the Macroscale to the Microscale. Angew Chem Int Ed Engl. 2015 Jul 13; 54(29):8340-62. PMID: 26084034.
    Citations: 25     Fields:    Translation:HumansAnimals
  16. Li Z, Roussakis E, Koolen PG, Ibrahim AM, Kim K, Rose LF, Wu J, Nichols AJ, Baek Y, Birngruber R, Apiou-Sbirlea G, Matyal R, Huang T, Chan R, Lin SJ, Evans CL. Non-invasive transdermal two-dimensional mapping of cutaneous oxygenation with a rapid-drying liquid bandage. Biomed Opt Express. 2014 Nov 01; 5(11):3748-64. PMID: 25426308; PMCID: PMC4242015.
    Citations: 16     
  17. Roussakis E, Spencer JA, Lin CP, Vinogradov SA. Two-photon antenna-core oxygen probe with enhanced performance. Anal Chem. 2014 Jun 17; 86(12):5937-45. PMID: 24848643.
    Citations: 25     Fields:    
  18. Nichols AJ, Roussakis E, Klein OJ, Evans CL. Click-assembled, oxygen-sensing nanoconjugates for depth-resolved, near-infrared imaging in a 3D cancer model. Angew Chem Int Ed Engl. 2014 Apr 01; 53(14):3671-4. PMID: 24590700.
    Citations: 13     Fields:    Translation:Humans
  19. Spencer JA, Ferraro F, Roussakis E, Klein A, Wu J, Runnels JM, Zaher W, Mortensen LJ, Alt C, Turcotte R, Yusuf R, Côté D, Vinogradov SA, Scadden DT, Lin CP. Direct measurement of local oxygen concentration in the bone marrow of live animals. Nature. 2014 Apr 10; 508(7495):269-73. PMID: 24590072.
    Citations: 419     Fields:    Translation:AnimalsCells
  20. Devor A, Sakadžic S, Yaseen MA, Roussakis E, Tian P, Slovin H, Vanzetta I, Teng I, Saisan PA, Sinks LE, Dale AM, Vinogradov SA, Boas DA. Optical Imaging of Neocortical Dynamics (Weber B., Helmchen F. (eds)). Functional Imaging of Cerebral Oxygenation with Intrinsic Optical Contrast and Phosphorescent Probes. 2014; 85:225-253. View Publication.
  21. Kazmi SM, Salvaggio AJ, Estrada AD, Hemati MA, Shaydyuk NK, Roussakis E, Jones TA, Vinogradov SA, Dunn AK. Three-dimensional mapping of oxygen tension in cortical arterioles before and after occlusion. Biomed Opt Express. 2013 Jul 01; 4(7):1061-73. PMID: 23847732.
    Citations: 24     
  22. Jung Y, Nichols AJ, Klein OJ, Roussakis E, Evans CL. Label-Free, Longitudinal Visualization of PDT Response In Vitro with Optical Coherence Tomography. Isr J Chem. 2012 Sep; 52(8-9):728-744. PMID: 23316088.
    Citations: 11     
  23. Devor A, Sakadzic S, Saisan PA, Yaseen MA, Roussakis E, Srinivasan VJ, Vinogradov SA, Rosen BR, Buxton RB, Dale AM, Boas DA. "Overshoot" of O2 is required to maintain baseline tissue oxygenation at locations distal to blood vessels. J Neurosci. 2011 Sep 21; 31(38):13676-81. PMID: 21940458.
    Citations: 89     Fields:    Translation:AnimalsCells
  24. Lecoq J, Parpaleix A, Roussakis E, Ducros M, Goulam Houssen Y, Vinogradov SA, Charpak S. Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels. Nat Med. 2011 Jun 05; 17(7):893-8. PMID: 21642977.
    Citations: 99     Fields:    Translation:Animals
  25. Sakadžic S, Roussakis E, Yaseen MA, Mandeville ET, Srinivasan VJ, Arai K, Ruvinskaya S, Wu W, Devor A, Lo EH, Vinogradov SA, Boas DA. Cerebral blood oxygenation measurement based on oxygen-dependent quenching of phosphorescence. J Vis Exp. 2011 May 04; (51). PMID: 21587157.
    Citations: 12     Fields:    Translation:Animals
  26. Sakadzic S, Roussakis E, Yaseen MA, Mandeville ET, Srinivasan VJ, Arai K, Ruvinskaya S, Devor A, Lo EH, Vinogradov SA, Boas DA. Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissue. Nat Methods. 2010 Sep; 7(9):755-9. PMID: 20693997.
    Citations: 156     Fields:    Translation:Animals
  27. Sinks LE, Robbins GP, Roussakis E, Troxler T, Hammer DA, Vinogradov SA. Two-photon microscopy of oxygen: polymersomes as probe carrier vehicles. J Phys Chem B. 2010 Nov 18; 114(45):14373-82. PMID: 20462225.
    Citations: 13     Fields:    
  28. Sinks LE, Roussakis E, Esipova TV, Vinogradov SA. Synthesis and calibration of phosphorescent nanoprobes for oxygen imaging in biological systems. J Vis Exp. 2010 Mar 03; (37). PMID: 20200497.
    Citations: 2     Fields:    Translation:AnimalsCells
  29. Roussakis E, Pergantis SA, Katerinopoulos HE. Coumarin-based ratiometric fluorescent indicators with high specificity for lead ions. Chem Commun (Camb). 2008 Dec 14; (46):6221-3. PMID: 19082126.
    Citations: 2     Fields:    Translation:Cells
  30. Roussakis E, Voutsadaki S, Pinakoulaki E, Sideris DP, Tokatlidis K, Katerinopoulos HE. ICPBCZin: a red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions. Cell Calcium. 2008 Sep; 44(3):270-5. PMID: 18243303.
    Citations: 3     Fields:    Translation:Cells
  31. Nifli AP, Theodoropoulos PA, Munier S, Castagnino C, Roussakis E, Katerinopoulos HE, Vercauteren J, Castanas E. Quercetin exhibits a specific fluorescence in cellular milieu: a valuable tool for the study of its intracellular distribution. J Agric Food Chem. 2007 Apr 18; 55(8):2873-8. PMID: 17385883.
    Citations: 7     Fields:    Translation:HumansAnimalsCells
  32. Roussakis E, Liepouri F, Nifli AP, Castanas E, Deligeorgiev TG, Katerinopoulos HE. ICPBC and C12-ICPBC: two new red emitting, fluorescent Ca2+ indicators excited with visible light. Cell Calcium. 2006 Jan; 39(1):3-11. PMID: 16236357.
    Citations: 1     Fields:    Translation:HumansCells
  33. Dakanali M, Roussakis E, Kay AR, Katerinopoulos HE. Synthesis and photophysical properties of a fluorescent TREN-type ligand incorporating the coumarin chromophore and its zinc complex. Tetrahedron Letters. 2005; 46(24):4193-4196. View Publication.
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Funded by the NIH National Center for Advancing Translational Sciences through its Clinical and Translational Science Awards Program, grant number UL1TR002541.