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Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
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Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase.
Glieder A, Farinas ET, Arnold FH. Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase. Nat Biotechnol. 2002 Nov; 20(11):1135-9.
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subject areas
Alcohols
Alkanes
Bacillus megaterium
Bacterial Proteins
Catalysis
Cytochrome P-450 Enzyme System
Enzyme Activation
Evolution, Molecular
Hydroxylation
Mixed Function Oxygenases
Mutagenesis, Site-Directed
Oxidation-Reduction
Proteomics
Quality Control
Recombinant Proteins
Sensitivity and Specificity
Solubility
Species Specificity
authors with profiles
Frances Lowe Arnold, Ph.D.