Associate Professor of Stem Cell and Regenerative Biology
The International journal of developmental biology
An integrative framework for salamander and mouse limb regeneration. Int J Dev Biol. 2018; 62(6-7-8):393-402.
An integrative framework for salamander and mouse limb regeneration.
Regeneration review reprise. J Biol. 2010; 9(2):15.
Leigh ND, Dunlap GS, Johnson K, Mariano R, Oshiro R, Wong AY, Bryant DM, Miller BM, Ratner A, Chen A, Ye WW, Haas BJ, Whited JL
Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution. Nat Commun. 2018 12 04; 9(1):5153.
Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution.
Limb regeneration revisited. J Biol. 2009; 8(1):5.
Proceedings of the National Academy of Sciences of the United States of America
Inducible genetic system for the axolotl. Proc Natl Acad Sci U S A. 2012 Aug 21; 109(34):13662-7.
Inducible genetic system for the axolotl.
TRANSLATING NEW INSIGHTS FROM AXOLOTL LIMB REGENERATION INTO MICE
Dorothy and Milton Flier Professor of Biomedical Science and Genetics
George Jacob and Jacqueline Hazel Leder Professor of Genetics
Professor of Stem Cell and Regenerative Biology
James Stillman Professor of Developmental Biology
Common themes in tetrapod appendage regeneration: a cellular perspective. Evodevo. 2019; 10:11.
Common themes in tetrapod appendage regeneration: a cellular perspective.
Salamander Models in Cross-Disciplinary Biological Research
IDENTIFYING ROADBLOCKS TO LIMB REGENERATION
Development: How Tadpoles ROC Tail Regeneration. Curr Biol. 2019 08 05; 29(15):R756-R758.
Development: How Tadpoles ROC Tail Regeneration.
Current opinion in genetics & development
Bioelectrical controls of morphogenesis: from ancient mechanisms of cell coordination to biomedical opportunities. Curr Opin Genet Dev. 2019 08; 57:61-69.
Bioelectrical controls of morphogenesis: from ancient mechanisms of cell coordination to biomedical opportunities.
Developmental dynamics : an official publication of the American Association of Anatomists
Discussing limb development and regeneration in Barcelona: The future is at hand. Dev Dyn. 2020 02; 249(2):160-163.
Discussing limb development and regeneration in Barcelona: The future is at hand.
de Girolamo L, Morlin Ambra LF, Perucca Orfei C, McQuilling JP, Kimmerling KA, Mowry KC, Johnson KA, Phan AT, Whited JL, Gomoll AH
Treatment with Human Amniotic Suspension Allograft Improves Tendon Healing in a Rat Model of Collagenase-Induced Tendinopathy. Cells. 2019 11 08; 8(11).
Treatment with Human Amniotic Suspension Allograft Improves Tendon Healing in a Rat Model of Collagenase-Induced Tendinopathy.
Parallels between wound healing, epimorphic regeneration and solid tumors. Development. 2020 01 02; 147(1).
Parallels between wound healing, epimorphic regeneration and solid tumors.
Tisza MJ, Pastrana DV, Welch NL, Stewart B, Peretti A, Starrett GJ, Pang YS, Krishnamurthy SR, Pesavento PA, McDermott DH, Murphy PM, Whited JL, Miller B, Brenchley J, Rosshart SP, Rehermann B, Doorbar J, Ta'ala BA, Pletnikova O, Troncoso JC, Resnick SM, Bolduc B, Sullivan MB, Varsani A, Segall AM, Buck CB
Discovery of several thousand highly diverse circular DNA viruses. Elife. 2020 02 04; 9.
Discovery of several thousand highly diverse circular DNA viruses.
Sousounis K, Bryant DM, Martinez Fernandez J, Eddy SS, Tsai SL, Gundberg GC, Han J, Courtemanche K, Levin M, Whited JL
Eya2 promotes cell cycle progression by regulating DNA damage response during vertebrate limb regeneration. Elife. 2020 03 06; 9.
Eya2 promotes cell cycle progression by regulating DNA damage response during vertebrate limb regeneration.
Leigh ND, Sessa S, Dragalzew AC, Payzin-Dogru D, Sousa JF, Aggouras AN, Johnson K, Dunlap GS, Haas BJ, Levin M, Schneider I, Whited JL
von Willebrand factor D and EGF domains is an evolutionarily conserved and required feature of blastemas capable of multitissue appendage regeneration. Evol Dev. 2020 07; 22(4):297-311.
von Willebrand factor D and EGF domains is an evolutionarily conserved and required feature of blastemas capable of multitissue appendage regeneration.
Sousounis K, Erdogan B, Levin M, Whited JL
Precise control of ion channel and gap junction expression is required for patterning of the regenerating axolotl limb. Int J Dev Biol. 2020; 64(10-11-12):485-494.
Precise control of ion channel and gap junction expression is required for patterning of the regenerating axolotl limb.
Engineered myosins drive filopodial transport. Nat Cell Biol. 2021 02; 23(2):113-115.
Engineered myosins drive filopodial transport.
A cross-species analysis of systemic mediators of repair and complex tissue regeneration. NPJ Regen Med. 2021 Apr 01; 6(1):21.
A cross-species analysis of systemic mediators of repair and complex tissue regeneration.
16th International Conference on Limb Development and Regeneration
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Finding Solutions for Fibrosis: Understanding the Innate Mechanisms Used by Super-Regenerator Vertebrates to Combat Scarring. Adv Sci (Weinh). 2021 08; 8(15):e2100407.
Finding Solutions for Fibrosis: Understanding the Innate Mechanisms Used by Super-Regenerator Vertebrates to Combat Scarring.
Salamander models for elucidating mechanisms of developmental biology, evolution, and regeneration: Part one. Dev Dyn. 2021 06; 250(6):750-752.
Salamander models for elucidating mechanisms of developmental biology, evolution, and regeneration: Part one.
Cable J, Elowitz MB, Domingos AI, Habib N, Itzkovitz S, Hamidzada H, Balzer MS, Yanai I, Liberali P, Whited J, Streets A, Cai L, Stergachis AB, Hong CKY, Keren L, Guilliams M, Alon U, Shalek AK, Hamel R, Pfau SJ, Raj A, Quake SR, Zhang NR, Fan J, Trapnell C, Wang B, Greenwald NF, Vento-Tormo R, Santos SDM, Spencer SL, Garcia HG, Arekatla G, Gaiti F, Arbel-Goren R, Rulands S, Junker JP, Klein AM, Morris SA, Murray JI, Galloway KE, Ratz M, Romeike M
Annals of the New York Academy of Sciences
Single cell biology-a Keystone Symposia report. Ann N Y Acad Sci. 2021 12; 1506(1):74-97.
Single cell biology-a Keystone Symposia report.
Salamander models for elucidating mechanisms of developmental biology, evolution, and regeneration: Part two. Dev Dyn. 2022 06; 251(6):903-905.
Salamander models for elucidating mechanisms of developmental biology, evolution, and regeneration: Part two.
Methods in molecular biology (Clifton, N.J.)
A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls. Methods Mol Biol. 2023; 2562:335-349.
A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.
Limb blastema formation: How much do we know at a genetic and epigenetic level? J Biol Chem. 2023 02; 299(2):102858.
Limb blastema formation: How much do we know at a genetic and epigenetic level?
Bhuiyan SA, Xu M, Yang L, Semizoglou E, Bhatia P, Pantaleo KI, Tochitsky I, Jain A, Erdogan B, Blair S, Cat V, Mwirigi JM, Sankaranarayanan I, Tavares-Ferreira D, Green U, McIlvried LA, Copits BA, Bertels Z, Del Rosario JS, Widman AJ, Slivicki RA, Yi J, Woolf CJ, Lennerz JK, Whited JL, Price TJ, Gereau RW, Renthal W
bioRxiv : the preprint server for biology
Harmonized cross-species cell atlases of trigeminal and dorsal root ganglia. bioRxiv. 2023 Jul 05.
Harmonized cross-species cell atlases of trigeminal and dorsal root ganglia.
Frontiers in cell and developmental biology
The salamander blastema within the broader context of metazoan regeneration. Front Cell Dev Biol. 2023; 11:1206157.
The salamander blastema within the broader context of metazoan regeneration.
James LM, Strickland Z, Lopez N, Whited JL, Maden M, Lewis J
Identification and Analysis of Axolotl Homologs for Proteins Implicated in Human Neurodegenerative Proteinopathies. Genes (Basel). 2024 02 28; 15(3).
Identification and Analysis of Axolotl Homologs for Proteins Implicated in Human Neurodegenerative Proteinopathies.
In preprints: cellular memory - the tension between old and new identities in the blastema. Development. 2024 Jan 01; 151(1).
In preprints: cellular memory - the tension between old and new identities in the blastema.
Roles of body-wide injury responses in axolotl limb regeneration
Bhuiyan SA, Xu M, Yang L, Semizoglou E, Bhatia P, Pantaleo KI, Tochitsky I, Jain A, Erdogan B, Blair S, Cat V, Mwirigi JM, Sankaranarayanan I, Tavares-Ferreira D, Green U, McIlvried LA, Copits BA, Bertels Z, Del Rosario JS, Widman AJ, Slivicki RA, Yi J, Sharif-Naeini R, Woolf CJ, Lennerz JK, Whited JL, Price TJ, Renthal W
Harmonized cross-species cell atlases of trigeminal and dorsal root ganglia. Sci Adv. 2024 06 21; 10(25):eadj9173.
An axolotl embryogenesis single-cell reference atlas to enable lineage-based developmental and regenerative studies
Automated aquatic husbandry equipment to enable rigorous and reproducible axolotl studies
Dooling KE, Kim RT, Kim EM, Chen E, Abouelela A, Tajer BJ, Lopez NJ, Paoli JC, Powell CJ, Luong AG, Wu SYC, Thornton KN, Singer HD, Savage AM, Bateman J, DiTommaso T, Payzin-Dogru D, Whited JL
Amputation Triggers Long-Range Epidermal Permeability Changes in Evolutionarily Distant Regenerative Organisms. bioRxiv. 2024 Aug 31.
Amputation Triggers Long-Range Epidermal Permeability Changes in Evolutionarily Distant Regenerative Organisms.
Putative epithelial-mesenchymal transitions during salamander limb regeneration: Current perspectives and future investigations. Ann N Y Acad Sci. 2024 Oct; 1540(1):89-103.
Putative epithelial-mesenchymal transitions during salamander limb regeneration: Current perspectives and future investigations.
Powell CJ, Singer HD, Juarez AR, Kim RT, Payzin-Dogru D, Savage AM, Lopez NJ, Blair SJ, Abouelela A, Dittrich A, Akeson SG, Jain M, Whited JL
Pancreatic injury induces ß-cell regeneration in axolotl. bioRxiv. 2025 Jan 23.
Pancreatic injury induces ß-cell regeneration in axolotl.
Dagdeviren S, Hoang MF, Wang J, Goldberger T, Yu AM, Blair SJ, Benoit JC, Ricci-Blair EM, Melnik VY, Kim BY, Tauer LA, Scholz JJ, Worthmann A, Schlein C, Stone D, Deuitch NT, Aksentijevich I, Schnappauf O, Whited JL, Kim JK, Lee RT
Covalent binding of thioredoxin to TXNIP is required for diet-induced insulin resistance in the liver. J Biol Chem. 2025 06; 301(6):110214.
Covalent binding of thioredoxin to TXNIP is required for diet-induced insulin resistance in the liver.
Tajer BJ, Kalu G, Jay S, Wynn E, Decaux A, Gilbert P, Singer HD, Kidd MD, Nelson JA, Harake N, Lopez NJ, Souchet NR, Luong AG, Savage AM, Min S, Karabacak A, Böhm S, Kim RT, Froitzheim T, Sousounis K, Courtemanche K, Han J, Payzin-Dogru D, Blair SJ, Roy S, Fei JF, Tanaka EM, Whited JL
Optimized toolkit for the manipulation of immortalized axolotl fibroblasts. Methods. 2025 Aug; 240:21-34.
Optimized toolkit for the manipulation of immortalized axolotl fibroblasts.
How axolotl cells 'remember' development to rebuild a lost limb. Nature. 2025 Jun; 642(8068):573-574.
How axolotl cells 'remember' development to rebuild a lost limb.
Amândio AR, Gaspar P, Whited JL, Janody F
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels. PLoS One. 2014; 9(5):e96326.
Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels.
Powell CJ, Singer HD, Juarez AR, Kim RT, Kim E, Payzin-Dogru D, Savage AM, Lopez NJ, Thornton K, Blair SJ, Abouelela A, Dittrich A, Akeson SG, Jain M, Whited JL
Pancreatic injury induces ß-cell regeneration in axolotl. Dev Dyn. 2025 Jul 18.
Savage AM, Wagner AC, Kim RT, Gilbert P, Singer HD, Chen E, Kim EM, Lopez N, Dooling KE, Paoli JC, Wu SYC, Böhm S, Froitzheim T, Chilambi R, Blair SJ, Powell CJ, Abouelela A, Luong AG, Thornton KN, Harake N, Karabacak A, Tajer B, Payzin-Dogru D, Whited JL
VEGF signaling promotes blastema growth and proliferation of vascular and non-vascular cells during axolotl limb regeneration. Dev Biol. 2025 Sep; 525:206-215.
VEGF signaling promotes blastema growth and proliferation of vascular and non-vascular cells during axolotl limb regeneration.
Payzin-Dogru D, Froitzheim T, Blair SJ, Jena SG, Singer H, Paoli JC, Kim RT, Kriukov E, Wilson SE, Hou R, Savage AM, Cat V, Cammarata LV, Wu SYC, Bothe V, Erdogan B, Hossain S, Lopez N, Losner J, Matos JV, Min S, Böhm S, Striker AE, Dooling KE, Freedman AH, Groves B, Tajer B, Kalu G, Wynn E, Wong AYL, Fröbisch N, Baranov P, Plikus MV, Buenrostro JD, Haas BJ, Chiu IM, Sackton TB, Whited JL
Adrenergic signaling coordinates distant and local responses to amputation in axolotl. bioRxiv. 2025 Jul 24.
Adrenergic signaling coordinates distant and local responses to amputation in axolotl.
Alvin and Esta Star Associate Professor of Stem Cell and Regenerative Biology
Pseudotyped retroviruses for infecting axolotl. Methods Mol Biol. 2015; 1290:127-40.
Pseudotyped retroviruses for infecting axolotl.
Defining the Mechanisms of Axon Growth after Limb Amputation
Farkas JE, Freitas PD, Bryant DM, Whited JL, Monaghan JR
Neuregulin-1 signaling is essential for nerve-dependent axolotl limb regeneration. Development. 2016 08 01; 143(15):2724-31.
Neuregulin-1 signaling is essential for nerve-dependent axolotl limb regeneration.
Kuo TH, Kowalko JE, DiTommaso T, Nyambi M, Montoro DT, Essner JJ, Whited JL
TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl. Regeneration (Oxf). 2015 Feb; 2(1):37-43.
TALEN-mediated gene editing of the thrombospondin-1 locus in axolotl.
Elucidating a Role for Blastema-Enriched Genes in Salamander Limb Regeneration,
EPIDERMAL FACTORS THAT PROMOTE INTERNAL TISSUE PROGENITOR ACTIVATION FOLLOWING AMPUTATION
Leveraging Single-Cell Analysis to Elucidate Mechanisms of Vertebrate LimbRegeneration
Cell Lineage Analysis in Vertebrate Limb Regeneration
Bryant DM, Johnson K, DiTommaso T, Tickle T, Couger MB, Payzin-Dogru D, Lee TJ, Leigh ND, Kuo TH, Davis FG, Bateman J, Bryant S, Guzikowski AR, Tsai SL, Coyne S, Ye WW, Freeman RM, Peshkin L, Tabin CJ, Regev A, Haas BJ, Whited JL
A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors. Cell Rep. 2017 01 17; 18(3):762-776.
A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors.
Bryant DM, Sousounis K, Farkas JE, Bryant S, Thao N, Guzikowski AR, Monaghan JR, Levin M, Whited JL
Repeated removal of developing limb buds permanently reduces appendage size in the highly-regenerative axolotl. Dev Biol. 2017 04 01; 424(1):1-9.
Repeated removal of developing limb buds permanently reduces appendage size in the highly-regenerative axolotl.
Whited JL, Cassell A, Brouillette M, Garrity PA
Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons. Development. 2004 Oct; 131(19):4677-86.
Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons.
Specifying axon identity with Syd-1. Nat Neurosci. 2002 Nov; 5(11):1107-8.
Saltman AJ, Barakat M, Bryant DM, Brodovskaya A, Whited JL
Journal of visualized experiments : JoVE
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum. J Vis Exp. 2017 06 16; (124).
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum.
Advances in Decoding Axolotl Limb Regeneration. Trends Genet. 2017 08; 33(8):553-565.
Advances in Decoding Axolotl Limb Regeneration.
Whited JL, Lehoczky JA, Austin CA, Tabin CJ
Dynamic expression of two thrombospondins during axolotl limb regeneration. Dev Dyn. 2011 May; 240(5):1249-58.
Dynamic expression of two thrombospondins during axolotl limb regeneration.
Whited JL, Robichaux MB, Yang JC, Garrity PA
Ptpmeg is required for the proper establishment and maintenance of axon projections in the central brain of Drosophila. Development. 2007 Jan; 134(1):43-53.
Ptpmeg is required for the proper establishment and maintenance of axon projections in the central brain of Drosophila.
Whited JL, Tsai SL, Beier KT, White JN, Piekarski N, Hanken J, Cepko CL, Tabin CJ
Pseudotyped retroviruses for infecting axolotl in vivo and in vitro. Development. 2013 Mar; 140(5):1137-46.
Pseudotyped retroviruses for infecting axolotl in vivo and in vitro.
Johnson K, Bateman J, DiTommaso T, Wong AY, Whited JL
Systemic cell cycle activation is induced following complex tissue injury in axolotl. Dev Biol. 2018 01 15; 433(2):461-472.
Systemic cell cycle activation is induced following complex tissue injury in axolotl.
Bryant DM, Sousounis K, Payzin-Dogru D, Bryant S, Sandoval AGW, Martinez Fernandez J, Mariano R, Oshiro R, Wong AY, Leigh ND, Johnson K, Whited JL
Identification of regenerative roadblocks via repeat deployment of limb regeneration in axolotls. NPJ Regen Med. 2017; 2:30.
Identification of regenerative roadblocks via repeat deployment of limb regeneration in axolotls.
Natarajan N, Abbas Y, Bryant DM, Gonzalez-Rosa JM, Sharpe M, Uygur A, Cocco-Delgado LH, Ho NN, Gerard NP, Gerard CJ, MacRae CA, Burns CE, Burns CG, Whited JL, Lee RT
Complement Receptor C5aR1 Plays an Evolutionarily Conserved Role in Successful Cardiac Regeneration. Circulation. 2018 05 15; 137(20):2152-2165.
Complement Receptor C5aR1 Plays an Evolutionarily Conserved Role in Successful Cardiac Regeneration.