Unsaturated phospholipid acyl chains are required to constitute membrane binding sites for factor VIII. Biochemistry. 1998 Sep 29; 37(39):13526-35.
Unsaturated phospholipid acyl chains are required to constitute membrane binding sites for factor VIII.
Sakurai Y, Hardy ET, Ahn B, Tran R, Fay ME, Ciciliano JC, Mannino RG, Myers DR, Qiu Y, Carden MA, Baldwin WH, Meeks SL, Gilbert GE, Jobe SM, Lam WA
A microengineered vascularized bleeding model that integrates the principal components of hemostasis. Nat Commun. 2018 02 06; 9(1):509.
A microengineered vascularized bleeding model that integrates the principal components of hemostasis.
Larsen E, Celi A, Gilbert GE, Furie BC, Erban JK, Bonfanti R, Wagner DD, Furie B
PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Cell. 1989 Oct 20; 59(2):305-12.
PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.
The evolving understanding of factor VIII binding sites and implications for the treatment of hemophilia A. Blood Rev. 2019 01; 33:1-5.
The evolving understanding of factor VIII binding sites and implications for the treatment of hemophilia A.
Grant MA, Baikeev RF, Gilbert GE, Rigby AC
Lysine 5 and phenylalanine 9 of the factor IX omega-loop interact with phosphatidylserine in a membrane-mimetic environment. Biochemistry. 2004 Dec 14; 43(49):15367-78.
Lysine 5 and phenylalanine 9 of the factor IX omega-loop interact with phosphatidylserine in a membrane-mimetic environment.
Meems H, Meijer AB, Cullinan DB, Mertens K, Gilbert GE
Factor VIII C1 domain residues Lys 2092 and Phe 2093 contribute to membrane binding and cofactor activity. Blood. 2009 Oct 29; 114(18):3938-46.
Factor VIII C1 domain residues Lys 2092 and Phe 2093 contribute to membrane binding and cofactor activity.
Phosphatidylethanolamine induces high affinity binding sites for factor VIII on membranes containing phosphatidyl-L-serine. J Biol Chem. 1995 Aug 04; 270(31):18500-5.
Phosphatidylethanolamine induces high affinity binding sites for factor VIII on membranes containing phosphatidyl-L-serine.
Shi J, Pipe SW, Rasmussen JT, Heegaard CW, Gilbert GE
Journal of thrombosis and haemostasis : JTH
Lactadherin blocks thrombosis and hemostasis in vivo: correlation with platelet phosphatidylserine exposure. J Thromb Haemost. 2008 Jul; 6(7):1167-74.
Lactadherin blocks thrombosis and hemostasis in vivo: correlation with platelet phosphatidylserine exposure.
Zhou J, Shi J, Hou J, Cao F, Zhang Y, Rasmussen JT, Heegaard CW, Gilbert GE
Phosphatidylserine exposure and procoagulant activity in acute promyelocytic leukemia. J Thromb Haemost. 2010 Apr; 8(4):773-82.
Phosphatidylserine exposure and procoagulant activity in acute promyelocytic leukemia.
Waehrens LN, Heegaard CW, Gilbert GE, Rasmussen JT
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Bovine lactadherin as a calcium-independent imaging agent of phosphatidylserine expressed on the surface of apoptotic HeLa cells. J Histochem Cytochem. 2009 Oct; 57(10):907-14.
Bovine lactadherin as a calcium-independent imaging agent of phosphatidylserine expressed on the surface of apoptotic HeLa cells.
Zhou J, Zheng Y, Shi J, Lu C, Hou J, Yu H, Qiao X, Qi S, Gilbert GE
Daunorubicin induces procoagulant response through phosphatidylserine exposure in red blood cells. Thromb Res. 2010 Feb; 125(2):178-83.
Daunorubicin induces procoagulant response through phosphatidylserine exposure in red blood cells.
Veterans Affairs Boston Healthcare System
Latham Family Professor of Biological Chemistry and Molecular Pharmacology
Professor of Pediatrics and Medicine, Emeritus
Shi Q, Carman CV, Chen Y, Sage PT, Xue F, Liang XM, Gilbert GE
Unexpected enhancement of FVIII immunogenicity by endothelial expression in lentivirus-transduced and transgenic mice. Blood Adv. 2020 05 26; 4(10):2272-2285.
Unexpected enhancement of FVIII immunogenicity by endothelial expression in lentivirus-transduced and transgenic mice.
Chatterjee M, Meeks S, Novakovic VA, Gilbert GE
Discordance between platelet-supported and vesicle-supported factor VIII activity in the presence of anti-C2 domain inhibitory antibodies. J Thromb Haemost. 2020 12; 18(12):3184-3193.
Discordance between platelet-supported and vesicle-supported factor VIII activity in the presence of anti-C2 domain inhibitory antibodies.
Procoagulant activities of skeletal and cardiac muscle myosin depend on contaminating phospholipid. Blood. 2020 11 19; 136(21):2469-2472.
Procoagulant activities of skeletal and cardiac muscle myosin depend on contaminating phospholipid.
Factor V mutation illuminates the dominant anticoagulant role and importance of an unidentified platelet modifier. J Thromb Haemost. 2021 05; 19(5):1168-1170.
Factor V mutation illuminates the dominant anticoagulant role and importance of an unidentified platelet modifier.
Factor VIII-antibody structure and membrane binding. Blood. 2021 05 27; 137(21):2866-2868.
Factor VIII-antibody structure and membrane binding.
Carman CV, Nikova DN, Sakurai Y, Shi J, Novakovic VA, Rasmussen JT, Lam WA, Gilbert GE
Membrane curvature and PS localize coagulation proteins to filopodia and retraction fibers of endothelial cells. Blood Adv. 2023 01 10; 7(1):60-72.
Membrane curvature and PS localize coagulation proteins to filopodia and retraction fibers of endothelial cells.
Unexpected bleeding with platelet phosphatidylserine exposure defect: new kindred motivates rethinking Scott syndrome. J Thromb Haemost. 2024 Aug; 22(8):2147-2149.
Unexpected bleeding with platelet phosphatidylserine exposure defect: new kindred motivates rethinking Scott syndrome.
Xie R, Gao C, Li W, Zhu J, Novakovic V, Wang J, Ma R, Zhou J, Gilbert GE, Shi J
Phagocytosis by macrophages and endothelial cells inhibits procoagulant and fibrinolytic activity of acute promyelocytic leukemia cells. Blood. 2012 Mar 08; 119(10):2325-34.
Phagocytosis by macrophages and endothelial cells inhibits procoagulant and fibrinolytic activity of acute promyelocytic leukemia cells.
Otzen DE, Blans K, Wang H, Gilbert GE, Rasmussen JT
Lactadherin binds to phosphatidylserine-containing vesicles in a two-step mechanism sensitive to vesicle size and composition. Biochim Biophys Acta. 2012 Apr; 1818(4):1019-27.
Lactadherin binds to phosphatidylserine-containing vesicles in a two-step mechanism sensitive to vesicle size and composition.
Nuzzio KM, Cullinan DB, Novakovic VA, Boettcher JM, Rienstra CM, Gilbert GE, Baleja JD
Backbone resonance assignments of the C2 domain of coagulation factor VIII. Biomol NMR Assign. 2013 Apr; 7(1):31-4.
Backbone resonance assignments of the C2 domain of coagulation factor VIII.
Gao C, Xie R, Li W, Zhou J, Liu S, Cao F, Liu Y, Ma R, Si Y, Liu Y, Bi Y, Gilbert GE, Shi J
Endothelial cell phagocytosis of senescent neutrophils decreases procoagulant activity. Thromb Haemost. 2013 Jun; 109(6):1079-90.
Endothelial cell phagocytosis of senescent neutrophils decreases procoagulant activity.
Lü J, Pipe SW, Miao H, Jacquemin M, Gilbert GE
A membrane-interactive surface on the factor VIII C1 domain cooperates with the C2 domain for cofactor function. Blood. 2011 Mar 17; 117(11):3181-9.
A membrane-interactive surface on the factor VIII C1 domain cooperates with the C2 domain for cofactor function.
Curbing an inhibitor for hemostasis. Blood. 2011 May 19; 117(20):5277-8.
Novakovic VA, Cullinan DB, Wakabayashi H, Fay PJ, Baleja JD, Gilbert GE
Membrane-binding properties of the Factor VIII C2 domain. Biochem J. 2011 Apr 01; 435(1):187-96.
Membrane-binding properties of the Factor VIII C2 domain.
Fu Y, Zhou J, Li H, Cao F, Su Y, Fan S, Li Y, Wang S, Li L, Gilbert GE, Shi J
Daunorubicin induces procoagulant activity of cultured endothelial cells through phosphatidylserine exposure and microparticles release. Thromb Haemost. 2010 Dec; 104(6):1235-41.
Daunorubicin induces procoagulant activity of cultured endothelial cells through phosphatidylserine exposure and microparticles release.
Gilbert GE, Novakovic VA, Kaufman RJ, Miao H, Pipe SW
Conservative mutations in the C2 domains of factor VIII and factor V alter phospholipid binding and cofactor activity. Blood. 2012 Aug 30; 120(9):1923-32.
Conservative mutations in the C2 domains of factor VIII and factor V alter phospholipid binding and cofactor activity.
Nyegaard S, Novakovic VA, Rasmussen JT, Gilbert GE
Lactadherin inhibits secretory phospholipase A2 activity on pre-apoptotic leukemia cells. PLoS One. 2013; 8(10):e77143.
Lactadherin inhibits secretory phospholipase A2 activity on pre-apoptotic leukemia cells.
Factor VIII inhibitor epitopes and an enigma. Blood. 2013 Dec 19; 122(26):4160-1.
Factor VIII inhibitor epitopes and an enigma.
Pantazatos D, Gessner CR, Woods VL, Gilbert GE
Changes in the Factor VIII C2 domain upon membrane binding determined by hydrogen-deuterium exchange MS. Biochem J. 2014 Aug 01; 461(3):443-51.
Changes in the Factor VIII C2 domain upon membrane binding determined by hydrogen-deuterium exchange MS.
Gilbert GE, Novakovic VA, Shi J, Rasmussen J, Pipe SW
Platelet binding sites for factor VIII in relation to fibrin and phosphatidylserine. Blood. 2015 Sep 03; 126(10):1237-44.
Platelet binding sites for factor VIII in relation to fibrin and phosphatidylserine.
Gao C, Xie R, Yu C, Ma R, Dong W, Meng H, Zhang Y, Si Y, Zhang Z, Novakovic V, Zhang Y, Kou J, Bi Y, Li B, Xie R, Gilbert GE, Zhou J, Shi J
Thrombotic Role of Blood and Endothelial Cells in Uremia through Phosphatidylserine Exposure and Microparticle Release. PLoS One. 2015; 10(11):e0142835.
Thrombotic Role of Blood and Endothelial Cells in Uremia through Phosphatidylserine Exposure and Microparticle Release.
PLATELET PROTEINS ASSOCIATED WITH PLATELET ACTIVATION
Endothelial topography, phosphatidylserine, and procoagulant activity
FUNCTION OF FACTOR VIII ON THE PLATELET MEMBRANE
COORDINATE FUNCTION OF ENZYME COMPLEXES IN HEMOSTASIS
Inhibitory antibodies against factor VIII C1 domain. Blood. 2016 10 20; 128(16):2001-2002.
Inhibitory antibodies against factor VIII C1 domain.
Saenko EL, Shima M, Gilbert GE, Scandella D
Slowed release of thrombin-cleaved factor VIII from von Willebrand factor by a monoclonal and a human antibody is a novel mechanism for factor VIII inhibition. J Biol Chem. 1996 Nov 01; 271(44):27424-31.
Slowed release of thrombin-cleaved factor VIII from von Willebrand factor by a monoclonal and a human antibody is a novel mechanism for factor VIII inhibition.
Partial activation of the factor VIIIa-factor IXa enzyme complex by dihexanoic phosphatidylserine at submicellar concentrations. Biochemistry. 1997 Sep 02; 36(35):10768-76.
Partial activation of the factor VIIIa-factor IXa enzyme complex by dihexanoic phosphatidylserine at submicellar concentrations.
Role of the liver in regulating numbers of circulating neutrophils. Blood. 2001 Aug 15; 98(4):1226-30.
Role of the liver in regulating numbers of circulating neutrophils.
Gilbert GE, Kaufman RJ, Arena AA, Miao H, Pipe SW
Four hydrophobic amino acids of the factor VIII C2 domain are constituents of both the membrane-binding and von Willebrand factor-binding motifs. J Biol Chem. 2002 Feb 22; 277(8):6374-81.
Four hydrophobic amino acids of the factor VIII C2 domain are constituents of both the membrane-binding and von Willebrand factor-binding motifs.
Blostein MD, Rigby AC, Furie BC, Furie B, Gilbert GE
Amphipathic helices support function of blood coagulation factor IXa. Biochemistry. 2000 Oct 03; 39(39):12000-6.
Amphipathic helices support function of blood coagulation factor IXa.
Fibrin stimulates platelets to increase factor VIIIa binding site expression. J Thromb Haemost. 2004 Oct; 2(10):1806-15.
Fibrin stimulates platelets to increase factor VIIIa binding site expression.
Binding of human factor VIII to phospholipid vesicles. J Biol Chem. 1990 Jan 15; 265(2):815-22.
Binding of human factor VIII to phospholipid vesicles.
Bendetowicz AV, Morris JA, Wise RJ, Gilbert GE, Kaufman RJ
Binding of factor VIII to von willebrand factor is enabled by cleavage of the von Willebrand factor propeptide and enhanced by formation of disulfide-linked multimers. Blood. 1998 Jul 15; 92(2):529-38.
Binding of factor VIII to von willebrand factor is enabled by cleavage of the von Willebrand factor propeptide and enhanced by formation of disulfide-linked multimers.
Lactadherin inhibits enzyme complexes of blood coagulation by competing for phospholipid-binding sites. Blood. 2003 Apr 01; 101(7):2628-36.
Lactadherin inhibits enzyme complexes of blood coagulation by competing for phospholipid-binding sites.
Where does von Willebrand factor go? Blood. 2008 Sep 01; 112(5):1549-50.
Shi J, Heegaard CW, Rasmussen JT, Gilbert GE
Lactadherin binds selectively to membranes containing phosphatidyl-L-serine and increased curvature. Biochim Biophys Acta. 2004 Nov 17; 1667(1):82-90.
Lactadherin binds selectively to membranes containing phosphatidyl-L-serine and increased curvature.
Zhou J, Liu S, Ma M, Hou J, Yu H, Lu C, Gilbert GE, Shi J
Procoagulant activity and phosphatidylserine of amniotic fluid cells. Thromb Haemost. 2009 May; 101(5):845-51.
Procoagulant activity and phosphatidylserine of amniotic fluid cells.
Bardelle C, Furie B, Furie BC, Gilbert GE
Membrane binding kinetics of factor VIII indicate a complex binding process. J Biol Chem. 1993 Apr 25; 268(12):8815-24.
Membrane binding kinetics of factor VIII indicate a complex binding process.
Shi J, Shi Y, Waehrens LN, Rasmussen JT, Heegaard CW, Gilbert GE
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Lactadherin detects early phosphatidylserine exposure on immortalized leukemia cells undergoing programmed cell death. Cytometry A. 2006 Dec 01; 69(12):1193-201.
Lactadherin detects early phosphatidylserine exposure on immortalized leukemia cells undergoing programmed cell death.
Shao C, Novakovic VA, Head JF, Seaton BA, Gilbert GE
Crystal structure of lactadherin C2 domain at 1.7A resolution with mutational and computational analyses of its membrane-binding motif. J Biol Chem. 2008 Mar 14; 283(11):7230-41.
Crystal structure of lactadherin C2 domain at 1.7A resolution with mutational and computational analyses of its membrane-binding motif.
Shcherbina A, Cooley J, Lutskiy MI, Benarafa C, Gilbert GE, Remold-O'Donnell E
WASP plays a novel role in regulating platelet responses dependent on alphaIIbbeta3 integrin outside-in signalling. Br J Haematol. 2010 Feb; 148(3):416-27.
WASP plays a novel role in regulating platelet responses dependent on alphaIIbbeta3 integrin outside-in signalling.
Structure and topography of the membrane-binding C2 domain of factor VIII in the presence of dodecylphosphocholine micelles. Biochem J. 1998 Jun 01; 332 ( Pt 2):549-55.
Structure and topography of the membrane-binding C2 domain of factor VIII in the presence of dodecylphosphocholine micelles.
Gilbert GE, Sims PJ, Wiedmer T, Furie B, Furie BC, Shattil SJ
Platelet-derived microparticles express high affinity receptors for factor VIII. J Biol Chem. 1991 Sep 15; 266(26):17261-8.
Platelet-derived microparticles express high affinity receptors for factor VIII.
Gary E Gilbert Jialan Shi Christian W. Heegaard Jan T Rasmussen
Method, composition, and kit for detecting phosphatidylserine(PS) on cell membranes
Method, composition, and kit for detecting phosphatidylserine(PS) on cell membranes. 2004.
Scandella D, Gilbert GE, Shima M, Nakai H, Eagleson C, Felch M, Prescott R, Rajalakshmi KJ, Hoyer LW, Saenko E
Some factor VIII inhibitor antibodies recognize a common epitope corresponding to C2 domain amino acids 2248 through 2312, which overlap a phospholipid-binding site. Blood. 1995 Sep 01; 86(5):1811-9.
Some factor VIII inhibitor antibodies recognize a common epitope corresponding to C2 domain amino acids 2248 through 2312, which overlap a phospholipid-binding site.
Membrane-binding peptide from the C2 domain of factor VIII forms an amphipathic structure as determined by NMR spectroscopy. Biochemistry. 1995 Mar 07; 34(9):3022-31.
Membrane-binding peptide from the C2 domain of factor VIII forms an amphipathic structure as determined by NMR spectroscopy.
Collagen-bound von Willebrand factor has reduced affinity for factor VIII. J Biol Chem. 1999 Apr 30; 274(18):12300-7.
Collagen-bound von Willebrand factor has reduced affinity for factor VIII.
Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, Grinstein S
Membrane phosphatidylserine regulates surface charge and protein localization. Science. 2008 Jan 11; 319(5860):210-3.
Membrane phosphatidylserine regulates surface charge and protein localization.
Larsen E, Palabrica T, Sajer S, Gilbert GE, Wagner DD, Furie BC, Furie B
PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15). Cell. 1990 Nov 02; 63(3):467-74.
PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15).
Use of microsphere-supported phospholipid membranes for analysis of protein-lipid interactions. Curr Protoc Cytom. 2005 Nov; Chapter 13:Unit13.3.
Use of microsphere-supported phospholipid membranes for analysis of protein-lipid interactions.
Specific membrane binding of factor VIII is mediated by O-phospho-L-serine, a moiety of phosphatidylserine. Biochemistry. 1993 Sep 21; 32(37):9577-85.
Specific membrane binding of factor VIII is mediated by O-phospho-L-serine, a moiety of phosphatidylserine.
Activation of the factor VIIIa-factor IXa enzyme complex of blood coagulation by membranes containing phosphatidyl-L-serine. J Biol Chem. 1996 May 10; 271(19):11120-5.
Activation of the factor VIIIa-factor IXa enzyme complex of blood coagulation by membranes containing phosphatidyl-L-serine.
Use of lactadherin as an anticoagulant. 2003.
Gilbert GE, Drinkwater D, Barter S, Clouse SB
Specificity of phosphatidylserine-containing membrane binding sites for factor VIII. Studies with model membranes supported by glass microspheres (lipospheres). J Biol Chem. 1992 Aug 05; 267(22):15861-8.
Specificity of phosphatidylserine-containing membrane binding sites for factor VIII. Studies with model membranes supported by glass microspheres (lipospheres).