Professor of Biological Chemistry and Molecular Pharmacology
Proceedings of the National Academy of Sciences of the United States of America
The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components. Proc Natl Acad Sci U S A. 1999 Oct 26; 96(22):12679-84.
The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components.
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation. Cell. 1990 Feb 23; 60(4):649-64.
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
Choi KY, Satterberg B, Lyons DM, Elion EA
Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell. 1994 Aug 12; 78(3):499-512.
Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae.
Localized feedback phosphorylation of Ste5p scaffold by associated MAPK cascade. J Biol Chem. 2004 Nov 05; 279(45):47391-401.
Localized feedback phosphorylation of Ste5p scaffold by associated MAPK cascade.
Detection of protein-protein interactions by coprecipitation. Curr Protoc Immunol. 2007 Feb; Chapter 8:8.7.1-8.7.10.
Detection of protein-protein interactions by coprecipitation.
A role for autophosphorylation revealed by activated alleles of FUS3, the yeast MAP kinase homolog. Mol Biol Cell. 1994 Mar; 5(3):297-312.
A role for autophosphorylation revealed by activated alleles of FUS3, the yeast MAP kinase homolog.
Feng Y, Song LY, Kincaid E, Mahanty SK, Elion EA
Functional binding between Gbeta and the LIM domain of Ste5 is required to activate the MEKK Ste11. Curr Biol. 1998 Feb 26; 8(5):267-78.
Functional binding between Gbeta and the LIM domain of Ste5 is required to activate the MEKK Ste11.
far4, far5, and far6 define three genes required for efficient activation of MAPKs Fus3 and Kss1 and accumulation of glycogen. Curr Genet. 2001 Aug; 40(1):13-26.
far4, far5, and far6 define three genes required for efficient activation of MAPKs Fus3 and Kss1 and accumulation of glycogen.
Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation. J Cell Biol. 1995 Sep; 130(6):1283-96.
Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation.
Signal transduction. Signaling specificity in yeast. Science. 2005 Feb 04; 307(5710):687-8.
Signal transduction. Signaling specificity in yeast.
Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene. BMC Mol Biol. 2011 Nov 24; 12:51.
Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene.
An RNA polymerase I enhancer in Saccharomyces cerevisiae. Mol Cell Biol. 1986 Jun; 6(6):2089-97.
An RNA polymerase I enhancer in Saccharomyces cerevisiae.
Hamilton Kuhn Professor of Biological Chemistry and Molecular Pharmacology
Lecturer on Biological Chemistry and Molecular Pharmacology
David Wesley Gaiser Professor of Biological Chemistry and Molecular Pharmacology
Don W. Fawcett Professor of Cell Biology
Research Associate in Biological Chemistry and Molecular Pharmacology
Instructor in Biological Chemistry and Molecular Pharmacology
Farley FW, McCully RR, Maslo PB, Yu L, Sheff MA, Sadeghi H, Elion EA
Effects of HSP70 chaperones Ssa1 and Ssa2 on Ste5 scaffold and the mating mitogen-activated protein kinase (MAPK) pathway in Saccharomyces cerevisiae. PLoS One. 2023; 18(10):e0289339.
Effects of HSP70 chaperones Ssa1 and Ssa2 on Ste5 scaffold and the mating mitogen-activated protein kinase (MAPK) pathway in Saccharomyces cerevisiae.
Making protein immunoprecipitates. Methods in Molecular Biology 2004; 284: 1-14
Making protein immunoprecipitates. Methods in Molecular Biology 2004; 284: 1-14.
Co-precipitating proteins. Current Protocols in Molecular Biology, Ausubel,F, Brent R Kingston R, Moore D, Smith JA, Seidman J, Struhl K, editors.. Guilford, CT: Greene Publishing Associates and Wiley
Co-precipitating proteins. Current Protocols in Molecular Biology, Ausubel,F, Brent R Kingston R, Moore D, Smith JA, Seidman J, Struhl K, editors.. Guilford, CT: Greene Publishing Associates and Wiley. 2000.
Characterization of a yeast RNA polymerase I enhancer. Transcriptional Control Mechanisms, UCLA Symposia on Molecular and Cellular Biology, New Series, Volume 52, Granner DK, Rosenfeld G and Chang S, editors
Characterization of a yeast RNA polymerase I enhancer. Transcriptional Control Mechanisms, UCLA Symposia on Molecular and Cellular Biology, New Series, Volume 52, Granner DK, Rosenfeld G and Chang S, editors. 1985.
Co-precipitating proteins. For: Signal Transduction Protocols in Methods in Molecular Biology, R.C. Dickson RC and Mendenhall MD, editors. Human Press
Co-precipitating proteins. For: Signal Transduction Protocols in Methods in Molecular Biology, R.C. Dickson RC and Mendenhall MD, editors. Human Press. 2004.
Warner J R, Elion E A, Dabeva M D, and Schwindinger WF
Ribosomes: Genetics, Structure and Function
The ribosomal genes of yeast and their regulation. Ribosomes: Genetics, Structure and Function. 1988.
The ribosomal genes of yeast and their regulation
Fillit H, Elion E, Sullivan J, Sherman R, Zabriskie JB
Thiobarbituric acid reactive material in uremic blood. Nephron. 1981; 29(1-2):40-3.
Thiobarbituric acid reactive material in uremic blood.
MAP kinase in yeast. Handbook of Cellular Signaling, Bradshaw R. and Dennis E, editors, T. Hunter T and Scott JD, associate editors. Academic Press
MAP kinase in yeast. Handbook of Cellular Signaling, Bradshaw R. and Dennis E, editors, T. Hunter T and Scott JD, associate editors. Academic Press. 2008.
MAP Kinase Modules in Signaling, Biomedical Sciences - Cancer and Endocrine Diseases, in the section Pathobiology of Cancer and Neoplasia, pp1-30 Michael Caplan, editor. Elsevier, Inc., Waltham, MA in press
MAP Kinase Modules in Signaling, Biomedical Sciences - Cancer and Endocrine Diseases, in the section Pathobiology of Cancer and Neoplasia, pp1-30 Michael Caplan, editor. Elsevier, Inc., Waltham, MA in press. 2015.
MAP Kinase Modules in Signaling; Encyclopedia of Cancer, Second Edition I. Biology of Cancer, Wang J, editor. San Diego, California: Academic Press
MAP Kinase Modules in Signaling; Encyclopedia of Cancer, Second Edition I. Biology of Cancer, Wang J, editor. San Diego, California: Academic Press. 2002.
FUS3. In: Protein Kinase Factsbook, Hanks S and Hardie DG, editors, Academic Press
FUS3. In: Protein Kinase Factsbook, Hanks S and Hardie DG, editors, Academic Press. 1988.
MAP kinase in yeast. Handbook of Cellular Signaling, Bradshaw R. and Dennis E, editors, T. Hunter T and Scott JD, associate editors. Academic Press. 2003.
Functional elements of the yeast rRNA transcription uni
Sequence Specificty in Transcription and Translation, UCLA Symposia on Molecular and Cellular Biology, New Series, Calendar R and Gold L, editors. Functional elements of the yeast rRNA transcription uni. 1985; 30.
Sequence Specificty in Transcription and Translation, UCLA Symposia on Molecular and Cellular Biology, New Series, Calendar R and Gold L, editors
Cherkasova V McCully R Wang Y Hinnebusch A and Elion EA.
A novel functional link between MAPKs and the Ras/cAMP pathway required for survival. Current Biology. 2003; (13):1214-1219.
A novel functional link between MAPKs and the Ras/cAMP pathway required for survival
. Using the polymerase chain reaction to construct recombinant DNA molecules (Unit 3.17) In: Current Protocols in Molecular Biology, Ausubel,F, Brent R Kingston R, Moore D, Smith JA, Seidman J, Struhl K, editors.. Guilford, CT: Greene Publishing Associates and Wiley
. Using the polymerase chain reaction to construct recombinant DNA molecules (Unit 3.17) In: Current Protocols in Molecular Biology, Ausubel,F, Brent R Kingston R, Moore D, Smith JA, Seidman J, Struhl K, editors.. Guilford, CT: Greene Publishing Associates and Wiley. 1995.
Hu Z, Wang Y, Yu L, Mahanty SK, Mendoza N, Elion EA
Biochemistry and cell biology = Biochimie et biologie cellulaire
Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export. Biochem Cell Biol. 2016 Apr; 94(2):109-28.
Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export.
Cell Cycle Arrest and Signal Transduction in Yeast
Lyons DM, Mahanty SK, Choi KY, Manandhar M, Elion EA
The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae. Mol Cell Biol. 1996 Aug; 16(8):4095-106.
The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae.
Hall JP, Cherkasova V, Elion E, Gustin MC, Winter E
The osmoregulatory pathway represses mating pathway activity in Saccharomyces cerevisiae: isolation of a FUS3 mutant that is insensitive to the repression mechanism. Mol Cell Biol. 1996 Dec; 16(12):6715-23.
The osmoregulatory pathway represses mating pathway activity in Saccharomyces cerevisiae: isolation of a FUS3 mutant that is insensitive to the repression mechanism.
Pheromone response, mating and cell biology. Curr Opin Microbiol. 2000 Dec; 3(6):573-81.
Pheromone response, mating and cell biology.
MAP kinase pathways. J Cell Sci. 2005 Aug 15; 118(Pt 16):3569-72.
Methods for analyzing MAPK cascades. Methods. 2006 Nov; 40(3):207-8.
Cherkasova VA, McCully R, Wang Y, Hinnebusch A, Elion EA
A novel functional link between MAP kinase cascades and the Ras/cAMP pathway that regulates survival. Curr Biol. 2003 Jul 15; 13(14):1220-6.
A novel functional link between MAP kinase cascades and the Ras/cAMP pathway that regulates survival.
Detection of protein-protein interactions by coprecipitation. Curr Protoc Protein Sci. 2007 Aug; Chapter 19:19.4.1-19.4.10.
How to monitor nuclear shuttling. Methods Enzymol. 2002; 351:607-22.
Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components. Mol Biol Cell. 2003 Jun; 14(6):2543-58.
Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components.
Cold Spring Harbor symposia on quantitative biology
Functional redundancy in the yeast cell cycle: FUS3 and KSS1 have both overlapping and unique functions. Cold Spring Harb Symp Quant Biol. 1991; 56:41-9.
Functional redundancy in the yeast cell cycle: FUS3 and KSS1 have both overlapping and unique functions.
Choi KY, Kranz JE, Mahanty SK, Park KS, Elion EA
Characterization of Fus3 localization: active Fus3 localizes in complexes of varying size and specific activity. Mol Biol Cell. 1999 May; 10(5):1553-68.
Characterization of Fus3 localization: active Fus3 localizes in complexes of varying size and specific activity.
Andersson J, Simpson DM, Qi M, Wang Y, Elion EA
Differential input by Ste5 scaffold and Msg5 phosphatase route a MAPK cascade to multiple outcomes. EMBO J. 2004 Jul 07; 23(13):2564-76.
Differential input by Ste5 scaffold and Msg5 phosphatase route a MAPK cascade to multiple outcomes.
Routing MAP kinase cascades. Science. 1998 Sep 11; 281(5383):1625-6.
Formin-induced actin cables are required for polarized recruitment of the Ste5 scaffold and high level activation of MAPK Fus3. J Cell Sci. 2005 Jul 01; 118(Pt 13):2837-48.
Formin-induced actin cables are required for polarized recruitment of the Ste5 scaffold and high level activation of MAPK Fus3.
The major promoter element of rRNA transcription in yeast lies 2 kb upstream. Cell. 1984 Dec; 39(3 Pt 2):663-73.
The major promoter element of rRNA transcription in yeast lies 2 kb upstream.
Detection of protein-protein interactions by coprecipitation. Curr Protoc Mol Biol. 2006 Nov; Chapter 20:Unit20.5.
Constructing recombinant DNA molecules by PCR. Curr Protoc Mol Biol. 2007 Apr; Chapter 3:Unit 3.17.
Constructing recombinant DNA molecules by PCR.
Methods in molecular biology (Clifton, N.J.)
Analysis of mitogen-activated protein kinase activity in yeast. Methods Mol Biol. 2010; 661:387-99.
Analysis of mitogen-activated protein kinase activity in yeast.
FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell. 1993 May; 4(5):495-510.
FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1.
POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2. Genetics. 1999 Feb; 151(2):531-43.
POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2.
The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5. Genes Dev. 1994 Feb 01; 8(3):313-27.
The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5.
Farley FW, Satterberg B, Goldsmith EJ, Elion EA
Relative dependence of different outputs of the Saccharomyces cerevisiae pheromone response pathway on the MAP kinase Fus3p. Genetics. 1999 Apr; 151(4):1425-44.
Relative dependence of different outputs of the Saccharomyces cerevisiae pheromone response pathway on the MAP kinase Fus3p.
Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade. Cell. 1999 Aug 20; 98(4):501-12.
Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade.
Ste5: a meeting place for MAP kinases and their associates. Trends Cell Biol. 1995 Aug; 5(8):322-7.
Ste5: a meeting place for MAP kinases and their associates.
FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc Natl Acad Sci U S A. 1991 Nov 01; 88(21):9392-6.
FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction.
The Ste5p scaffold. J Cell Sci. 2001 Nov; 114(Pt 22):3967-78.
Counteractive control of polarized morphogenesis during mating by mitogen-activated protein kinase Fus3 and G1 cyclin-dependent kinase. Mol Biol Cell. 2008 Apr; 19(4):1739-52.
Counteractive control of polarized morphogenesis during mating by mitogen-activated protein kinase Fus3 and G1 cyclin-dependent kinase.
Making protein immunoprecipitates. Methods Mol Biol. 2004; 284:1-14.
Detection of protein-protein interactions by coprecipitation. Curr Protoc Neurosci. 2006 May; Chapter 5:Unit 5.25.
Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999 Mar; 151(3):989-1004.
Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p.
Cdc24 regulates nuclear shuttling and recruitment of the Ste5 scaffold to a heterotrimeric G protein in Saccharomyces cerevisiae. J Biol Chem. 2005 Apr 01; 280(13):13084-96.
Cdc24 regulates nuclear shuttling and recruitment of the Ste5 scaffold to a heterotrimeric G protein in Saccharomyces cerevisiae.