GHIRAN, IONITA CALIN;IVANOV, ALEXANDER R.
Effect of methodological and biological variability on molecular profiling of extracellular vesicles in cancer detection
Andersen MS, Lu S, Lopez GJ, Lassen AT, Shapiro NI, Ghiran IC
A Novel Implementation of Magnetic Levitation to Quantify Leukocyte Size, Morphology, and Magnetic Properties to Identify Patients With Sepsis. Shock. 2019 02; 51(2):147-152.
A Novel Implementation of Magnetic Levitation to Quantify Leukocyte Size, Morphology, and Magnetic Properties to Identify Patients With Sepsis.
Smith AS, Nowak RB, Zhou S, Giannetto M, Gokhin DS, Papoin J, Ghiran IC, Blanc L, Wan J, Fowler VM
Proceedings of the National Academy of Sciences of the United States of America
Myosin IIA interacts with the spectrin-actin membrane skeleton to control red blood cell membrane curvature and deformability. Proc Natl Acad Sci U S A. 2018 05 08; 115(19):E4377-E4385.
Myosin IIA interacts with the spectrin-actin membrane skeleton to control red blood cell membrane curvature and deformability.
Chen CH, Ghiran I, Beurskens FJ, Weaver G, Vincent JA, Nicholson-Weller A, Klickstein LB
Antibody CR1-2B11 recognizes a non-polymorphic epitope of human CR1 (CD35). Clin Exp Immunol. 2007 Jun; 148(3):546-54.
Antibody CR1-2B11 recognizes a non-polymorphic epitope of human CR1 (CD35).
Honczarenko M, Le Y, Swierkowski M, Ghiran I, Glodek AM, Silberstein LE
Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors. Stem Cells. 2006 Apr; 24(4):1030-41.
Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors.
Ogembo JG, Kannan L, Ghiran I, Nicholson-Weller A, Finberg RW, Tsokos GC, Fingeroth JD
Human complement receptor type 1/CD35 is an Epstein-Barr Virus receptor. Cell Rep. 2013 Feb 21; 3(2):371-85.
Human complement receptor type 1/CD35 is an Epstein-Barr Virus receptor.
Karnchanaphanurach P, Mirchev R, Ghiran I, Asara JM, Papahadjopoulos-Sternberg B, Nicholson-Weller A, Golan DE
C3b deposition on human erythrocytes induces the formation of a membrane skeleton-linked protein complex. J Clin Invest. 2009 Apr; 119(4):788-801.
C3b deposition on human erythrocytes induces the formation of a membrane skeleton-linked protein complex.
Giraldez MD, Spengler RM, Etheridge A, Godoy PM, Barczak AJ, Srinivasan S, De Hoff PL, Tanriverdi K, Courtright A, Lu S, Khoory J, Rubio R, Baxter D, Driedonks TAP, Buermans HPJ, Nolte-'t Hoen ENM, Jiang H, Wang K, Ghiran I, Wang YE, Van Keuren-Jensen K, Freedman JE, Woodruff PG, Laurent LC, Erle DJ, Galas DJ, Tewari M
Comprehensive multi-center assessment of small RNA-seq methods for quantitative miRNA profiling. Nat Biotechnol. 2018 09; 36(8):746-757.
Comprehensive multi-center assessment of small RNA-seq methods for quantitative miRNA profiling.
Giraldez MD, Spengler RM, Etheridge A, Godoy PM, Barczak AJ, Srinivasan S, Hoff PL, Tanriverdi K, Courtright A, Lu S, Khoory J, Rubio R, Baxter D, Driedonks TAP, Buermans HPJ, Hoen ENMN, Jiang H, Wang K, Ghiran I, Wang YE, Keuren-Jensen KV, Freedman JE, Woodruff PG, Laurent LC, Erle DJ, Galas DJ, Tewari M
Erratum: Comprehensive multi-center assessment of small RNA-seq methods for quantitative miRNA profiling. Nat Biotechnol. 2018 09 06; 36(9):899.
Erratum: Comprehensive multi-center assessment of small RNA-seq methods for quantitative miRNA profiling.
Li J, Wang JP, Ghiran I, Cerny A, Szalai AJ, Briles DE, Finberg RW
Complement receptor 1 expression on mouse erythrocytes mediates clearance of Streptococcus pneumoniae by immune adherence. Infect Immun. 2010 Jul; 78(7):3129-35.
Complement receptor 1 expression on mouse erythrocytes mediates clearance of Streptococcus pneumoniae by immune adherence.
Wang HB, Ghiran I, Matthaei K, Weller PF
Journal of immunology (Baltimore, Md. : 1950)
Airway eosinophils: allergic inflammation recruited professional antigen-presenting cells. J Immunol. 2007 Dec 01; 179(11):7585-92.
Airway eosinophils: allergic inflammation recruited professional antigen-presenting cells.
Neves JS, Perez SA, Spencer LA, Melo RC, Reynolds L, Ghiran I, Mahmudi-Azer S, Odemuyiwa SO, Dvorak AM, Moqbel R, Weller PF
Eosinophil granules function extracellularly as receptor-mediated secretory organelles. Proc Natl Acad Sci U S A. 2008 Nov 25; 105(47):18478-83.
Eosinophil granules function extracellularly as receptor-mediated secretory organelles.
Ghiran I, Klickstein LB, Nicholson-Weller A
Calreticulin is at the surface of circulating neutrophils and uses CD59 as an adaptor molecule. J Biol Chem. 2003 Jun 06; 278(23):21024-31.
Calreticulin is at the surface of circulating neutrophils and uses CD59 as an adaptor molecule.
Lim KH, Salahuddin S, Qiu L, Fang H, Vitkin E, Ghiran IC, Modell MD, Takoudes T, Itzkan I, Hanlon EB, Sachs BP, Perelman LT
Light-scattering spectroscopy differentiates fetal from adult nucleated red blood cells: may lead to noninvasive prenatal diagnosis. Opt Lett. 2009 May 01; 34(9):1483-5.
Light-scattering spectroscopy differentiates fetal from adult nucleated red blood cells: may lead to noninvasive prenatal diagnosis.
Celli JP, Turner BS, Afdhal NH, Keates S, Ghiran I, Kelly CP, Ewoldt RH, McKinley GH, So P, Erramilli S, Bansil R
Helicobacter pylori moves through mucus by reducing mucin viscoelasticity. Proc Natl Acad Sci U S A. 2009 Aug 25; 106(34):14321-6.
Helicobacter pylori moves through mucus by reducing mucin viscoelasticity.
Ghiran IC, Zeidel ML, Shevkoplyas SS, Burns JM, Tsokos GC, Kyttaris VC
SLE serum deposits C4d on red blood cells, decreases red blood cell membrane deformability, and promotes nitric oxide production. Arthritis Rheum. 2010 Nov 15.
SLE serum deposits C4d on red blood cells, decreases red blood cell membrane deformability, and promotes nitric oxide production.
Bioengineered red blood cells as extracellular vesicle-mediated delivery platforms for gene editing machinery
Mantel PY, Hoang AN, Goldowitz I, Potashnikova D, Hamza B, Vorobjev I, Ghiran I, Toner M, Irimia D, Ivanov AR, Barteneva N, Marti M
Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe. 2013 May 15; 13(5):521-534.
Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system.
Interdisciplinary Medicine and Biotechnology
Harvard T.H. Chan School of Public Health
John LaPorte Given Professor of Immunology and Infectious Diseases
William Bosworth Castle Professor of Medicine
Simon Christopher Robson, M.B.Ch.B., Ph.D.
Morales-Kastresana A, Musich TA, Welsh JA, Telford W, Demberg T, Wood JCS, Bigos M, Ross CD, Kachynski A, Dean A, Felton EJ, Van Dyke J, Tigges J, Toxavidis V, Parks DR, Overton WR, Kesarwala AH, Freeman GJ, Rosner A, Perfetto SP, Pasquet L, Terabe M, McKinnon K, Kapoor V, Trepel JB, Puri A, Kobayashi H, Yung B, Chen X, Guion P, Choyke P, Knox SJ, Ghiran I, Robert-Guroff M, Berzofsky JA, Jones JC
High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer. J Extracell Vesicles. 2019; 8(1):1597603.
High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer.
Integrative, multi-parametric characterization of the EV surface protein and nucleic acid landscape by nano-flow and sorting cytometry
Bei Y, Xu T, Lv D, Yu P, Xu J, Che L, Das A, Tigges J, Toxavidis V, Ghiran I, Shah R, Li Y, Zhang Y, Das S, Xiao J
Correction to: Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury. Basic Res Cardiol. 2019 Oct 08; 114(6):44.
Correction to: Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury.
Jones PS, Yekula A, Lansbury E, Small JL, Ayinon C, Mordecai S, Hochberg FH, Tigges J, Delcuze B, Charest A, Ghiran I, Balaj L, Carter BS
Characterization of plasma-derived protoporphyrin-IX-positive extracellular vesicles following 5-ALA use in patients with malignant glioma. EBioMedicine. 2019 Oct; 48:23-35.
Characterization of plasma-derived protoporphyrin-IX-positive extracellular vesicles following 5-ALA use in patients with malignant glioma.
Lee J, Vernet A, Redfield K, Lu S, Ghiran IC, Way JC, Silver PA
Rational Design of a Bifunctional AND-Gate Ligand To Modulate Cell-Cell Interactions. ACS Synth Biol. 2020 02 21; 9(2):191-197.
Rational Design of a Bifunctional AND-Gate Ligand To Modulate Cell-Cell Interactions.
Oliveira GP, Zigon E, Rogers G, Davodian D, Lu S, Jovanovic-Talisman T, Jones J, Tigges J, Tyagi S, Ghiran IC
Detection of Extracellular Vesicle RNA Using Molecular Beacons. iScience. 2020 Jan 24; 23(1):100782.
Detection of Extracellular Vesicle RNA Using Molecular Beacons.
Welsh JA, Van Der Pol E, Arkesteijn GJA, Bremer M, Brisson A, Coumans F, Dignat-George F, Duggan E, Ghiran I, Giebel B, Görgens A, Hendrix A, Lacroix R, Lannigan J, Libregts SFWM, Lozano-Andrés E, Morales-Kastresana A, Robert S, De Rond L, Tertel T, Tigges J, De Wever O, Yan X, Nieuwland R, Wauben MHM, Nolan JP, Jones JC
MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments. J Extracell Vesicles. 2020; 9(1):1713526.
MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments.
Rodosthenous RS, Hutchins E, Reiman R, Yeri AS, Srinivasan S, Whitsett TG, Ghiran I, Silverman MG, Laurent LC, Van Keuren-Jensen K, Das S
Profiling Extracellular Long RNA Transcriptome in Human Plasma and Extracellular Vesicles for Biomarker Discovery. iScience. 2020 Jun 26; 23(6):101182.
Profiling Extracellular Long RNA Transcriptome in Human Plasma and Extracellular Vesicles for Biomarker Discovery.
Sharda AV, Barr AM, Harrison JA, Wilkie AR, Fang C, Mendez LM, Ghiran IC, Italiano JE, Flaumenhaft R
VWF maturation and release are controlled by 2 regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. Blood. 2020 12 10; 136(24):2824-2837.
VWF maturation and release are controlled by 2 regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.
Nelson BC, Maragh S, Ghiran IC, Jones JC, DeRose PC, Elsheikh E, Vreeland WN, Wang L
Measurement and standardization challenges for extracellular vesicle therapeutic delivery vectors. Nanomedicine (Lond). 2020 09; 15(22):2149-2170.
Measurement and standardization challenges for extracellular vesicle therapeutic delivery vectors.
Srinivasan S, Treacy R, Herrero T, Olsen R, Leonardo TR, Zhang X, DeHoff P, To C, Poling LG, Fernando A, Leon-Garcia S, Knepper K, Tran V, Meads M, Tasarz J, Vuppala A, Park S, Laurent CD, Bui T, Cheah PS, Overcash RT, Ramos GA, Roeder H, Ghiran I, Parast M, Breakefield XO, Lueth AJ, Rust SR, Dufford MT, Fox AC, Hickok DE, Burchard J, Boniface JJ, Laurent LC
Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women. Cell Rep Med. 2020 05 19; 1(2).
Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic Women.
Li J, Salvador AM, Li G, Valkov N, Ziegler O, Yeri A, Yang Xiao C, Meechoovet B, Alsop E, Rodosthenous RS, Kundu P, Huan T, Levy D, Tigges J, Pico AR, Ghiran I, Silverman MG, Meng X, Kitchen R, Xu J, Van Keuren-Jensen K, Shah R, Xiao J, Das S
Mir-30d Regulates Cardiac Remodeling by Intracellular and Paracrine Signaling. Circ Res. 2021 01 08; 128(1):e1-e23.
Mir-30d Regulates Cardiac Remodeling by Intracellular and Paracrine Signaling.
Marie AL, Ray S, Lu S, Jones J, Ghiran I, Ivanov AR
High-Sensitivity Glycan Profiling of Blood-Derived Immunoglobulin G, Plasma, and Extracellular Vesicle Isolates with Capillary Zone Electrophoresis-Mass Spectrometry. Anal Chem. 2021 02 02; 93(4):1991-2002.
High-Sensitivity Glycan Profiling of Blood-Derived Immunoglobulin G, Plasma, and Extracellular Vesicle Isolates with Capillary Zone Electrophoresis-Mass Spectrometry.
Red Blood Cells shuttle beta amyloid between brain and heart: implications for the pathogenesis and the progression of Alzheimer's and Cardiomyopathy
Saha K, Sontheimer EJ, Brooks PJ, Dwinell MR, Gersbach CA, Liu DR, Murray SA, Tsai SQ, Wilson RC, Anderson DG, Asokan A, Banfield JF, Bankiewicz KS, Bao G, Bulte JWM, Bursac N, Campbell JM, Carlson DF, Chaikof EL, Chen ZY, Cheng RH, Clark KJ, Curiel DT, Dahlman JE, Deverman BE, Dickinson ME, Doudna JA, Ekker SC, Emborg ME, Feng G, Freedman BS, Gamm DM, Gao G, Ghiran IC, Glazer PM, Gong S, Heaney JD, Hennebold JD, Hinson JT, Khvorova A, Kiani S, Lagor WR, Lam KS, Leong KW, Levine JE, Lewis JA, Lutz CM, Ly DH, Maragh S, McCray PB, McDevitt TC, Mirochnitchenko O, Morizane R, Murthy N, Prather RS, Ronald JA, Roy S, Roy S, Sabbisetti V, Saltzman WM, Santangelo PJ, Segal DJ, Shimoyama M, Skala MC, Tarantal AF, Tilton JC, Truskey GA, Vandsburger M, Watts JK, Wells KD, Wolfe SA, Xu Q, Xue W, Yi G, Zhou J
The NIH Somatic Cell Genome Editing program. Nature. 2021 04; 592(7853):195-204.
The NIH Somatic Cell Genome Editing program.
Thompson L, Pinckney B, Lu S, Gregory M, Tigges J, Ghiran I
Journal of visualized experiments : JoVE
Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation. J Vis Exp. 2021 05 17; (171).
Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation.
Oliveira-Jr GP, Barbosa RH, Thompson L, Pinckney B, Murphy-Thornley M, Lu S, Jones J, Hansen CH, Tigges J, Wong WP, Ghiran IC
Electrophoretic mobility shift as a molecular beacon-based readout for miRNA detection. Biosens Bioelectron. 2021 Oct 01; 189:113307.
Electrophoretic mobility shift as a molecular beacon-based readout for miRNA detection.
GHIRAN, IONITA CALIN ;JONES, JENNIFER ;MILOSAVLJEVIC, ALEKSANDAR
Valkov N, Das A, Tucker NR, Li G, Salvador AM, Chaffin MD, Pereira De Oliveira Junior G, Kur I, Gokulnath P, Ziegler O, Yeri A, Lu S, Khamesra A, Xiao C, Rodosthenous R, Srinivasan S, Toxavidis V, Tigges J, Laurent LC, Momma S, Kitchen R, Ellinor P, Ghiran I, Das S
SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart. Life Sci Alliance. 2021 12; 4(12).
SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart.
Alsop E, Meechoovet B, Kitchen R, Sweeney T, Beach TG, Serrano GE, Hutchins E, Ghiran I, Reiman R, Syring M, Hsieh M, Courtright-Lim A, Valkov N, Whitsett TG, Rakela J, Pockros P, Rozowsky J, Gallego J, Huentelman MJ, Shah R, Nakaji P, Kalani MYS, Laurent L, Das S, Van Keuren-Jensen K
Frontiers in cell and developmental biology
A Novel Tissue Atlas and Online Tool for the Interrogation of Small RNA Expression in Human Tissues and Biofluids. Front Cell Dev Biol. 2022; 10:804164.
A Novel Tissue Atlas and Online Tool for the Interrogation of Small RNA Expression in Human Tissues and Biofluids.
Welsh JA, Killingsworth B, Kepley J, Traynor T, Cook S, Savage J, Marte J, Lee MJ, Maeng HM, Pleet ML, Magana S, Gorgens A, Maire CL, Lamszus K, Ricklefs FL, Merino MJ, Linehan WM, Greten T, Cooks T, Harris CC, Apolo A, Abdel-Mageed A, Ivanov AR, Trepel JB, Roth M, Tkach M, Milosavljevic A, Théry C, LeBlanc A, Berzofsky JA, Ruppin E, Aldape K, Camphausen K, Gulley JL, Ghiran I, Jacobson S, Jones JC
MPAPASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays. Cell Rep Methods. 2022 01 24; 2(1):100136.
MPAPASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays.
Li G, Chen T, Dahlman J, Eniola-Adefeso L, Ghiran IC, Kurre P, Lam WA, Lang JK, Marbán E, Martín P, Momma S, Moos M, Nelson DJ, Raffai RL, Ren X, Sluijter JPG, Stott SL, Vunjak-Novakovic G, Walker ND, Wang Z, Witwer KW, Yang PC, Lundberg MS, Ochocinska MJ, Wong R, Zhou G, Chan SY, Das S, Sundd P
Current challenges and future directions for engineering extracellular vesicles for heart, lung, blood and sleep diseases. J Extracell Vesicles. 2023 02; 12(2):e12305.
Current challenges and future directions for engineering extracellular vesicles for heart, lung, blood and sleep diseases.
Arce JE, Welsh JA, Cook S, Tigges J, Ghiran I, Jones JC, Jackson A, Roth M, Milosavljevic A
The NanoFlow Repository. Bioinformatics. 2023 06 01; 39(6).
Chornenkyy Y, Yamamoto T, Hara H, Stowell SR, Ghiran I, Robson SC, Cooper DKC
Future prospects for the clinical transfusion of pig red blood cells. Blood Rev. 2023 09; 61:101113.
Future prospects for the clinical transfusion of pig red blood cells.
LaPlante EL, Stürchler A, Fullem R, Chen D, Starner AC, Esquivel E, Alsop E, Jackson AR, Ghiran I, Pereira G, Rozowsky J, Chang J, Gerstein MB, Alexander RP, Roth ME, Franklin JL, Coffey RJ, Raffai RL, Mansuy IM, Stavrakis S, deMello AJ, Laurent LC, Wang YT, Tsai CF, Liu T, Jones J, Van Keuren-Jensen K, Van Nostrand E, Mateescu B, Milosavljevic A
exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers. Cell Genom. 2023 May 10; 3(5):100303.
exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers.
Chatterjee E, Rodosthenous RS, Kujala V, Gokulnath P, Spanos M, Lehmann HI, de Oliveira GP, Shi M, Miller-Fleming TW, Li G, Ghiran IC, Karalis K, Lindenfeld J, Mosley JD, Lau ES, Ho JE, Sheng Q, Shah R, Das S
Circulating extracellular vesicles in human cardiorenal syndrome promote renal injury in a kidney-on-chip system. JCI Insight. 2023 11 22; 8(22).
Circulating extracellular vesicles in human cardiorenal syndrome promote renal injury in a kidney-on-chip system.
de Oliveira Junior GP, Welsh JA, Pinckney B, Palu CC, Lu S, Zimmerman A, Barbosa RH, Sahu P, Noshin M, Gummuluru S, Tigges J, Jones JC, Ivanov AR, Ghiran IC
Human red blood cells release microvesicles with distinct sizes and protein composition that alter neutrophil phagocytosis. J Extracell Biol. 2023 Nov; 2(11).
Human red blood cells release microvesicles with distinct sizes and protein composition that alter neutrophil phagocytosis.
Zimmerman AJ, de Oliveira GP, Su X, Wood J, Fu Z, Pinckney B, Tigges J, Ghiran I, Ivanov AR
Multimode chromatography-based techniques for high purity isolation of extracellular vesicles from human blood plasma. J Extracell Biol. 2024 Mar; 3(3).
Multimode chromatography-based techniques for high purity isolation of extracellular vesicles from human blood plasma.
Kim J, Xu S, Jung SR, Nguyen A, Cheng Y, Zhao M, Fujimoto BS, Nelson W, Schiro P, Franklin JL, Higginbotham JN, Coffey RJ, Shi M, Vojtech LN, Hladik F, Tewari M, Tigges J, Ghiran I, Jovanovic-Talisman T, Laurent LC, Das S, Gololobova O, Witwer KW, Xu T, Charest A, Jensen KVK, Raffai RL, Jones JC, Welsh JA, Nolan JP, Chiu DT
Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer. J Extracell Vesicles. 2024 Aug; 13(8):e12498.
Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer.
Tran V, de Oliveira-Jr GP, Chidester S, Lu S, Pleet ML, Ivanov AR, Tigges J, Yang M, Jacobson S, Gonçalves MCB, Schmaier AA, Jones J, Ghiran IC
Choice of blood collection methods influences extracellular vesicles counts and miRNA profiling. J Extracell Biol. 2024 Oct; 3(10):e70008.
Choice of blood collection methods influences extracellular vesicles counts and miRNA profiling.
Su X, Júnior GPO, Marie AL, Gregus M, Figueroa-Navedo A, Ghiran IC, Ivanov AR
Enhanced proteomic profiling of human plasma-derived extracellular vesicles through charge-based fractionation to advance biomarker discovery potential. J Extracell Vesicles. 2024 Dec; 13(12):e70024.
Enhanced proteomic profiling of human plasma-derived extracellular vesicles through charge-based fractionation to advance biomarker discovery potential.
Okeoma CM, Premadasa LS, Tan CS, Ghiran IC, Mohan M
bioRxiv : the preprint server for biology
Cannabinoids shift the basal ganglia microRNA m 6 A methylation profile towards an anti-inflammatory phenotype in SIV-infected rhesus macaques. bioRxiv. 2024 Dec 09.
Cannabinoids shift the basal ganglia microRNA m 6 A methylation profile towards an anti-inflammatory phenotype in SIV-infected rhesus macaques.
Furuhashi K, Kakiuchi M, Ueda R, Oda H, Ummarino S, Ebralidze AK, Bassal MA, Meng C, Sato T, Lyu J, Han MG, Maruyama S, Watanabe Y, Sawa Y, Kato D, Wake H, Reizis B, Frangos JA, Owens DM, Tenen DG, Ghiran IC, Robson SC, Fujisaki J
Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance. Nature. 2025 02; 638(8049):206-215.
Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance.
Yang M, Hancco Zirena I, Kennedy QP, Patel A, Merrill-Skoloff G, Sack KD, Fulcidor E, Scartelli C, Guo S, Bekendam RH, Owegie OC, Xie H, Ghiran IC, Levy O, Lin L, Flaumenhaft R
Journal of thrombosis and haemostasis : JTH
Galloylated polyphenols represent a new class of antithrombotic agents with broad activity against thiol isomerases. J Thromb Haemost. 2025 Jun; 23(6):1850-1863.
Galloylated polyphenols represent a new class of antithrombotic agents with broad activity against thiol isomerases.
Melhorn MI, Brodsky AS, Estanislau J, Khoory JA, Illigens B, Hamachi I, Kurishita Y, Fraser AD, Nicholson-Weller A, Dolmatova E, Duffy HS, Ghiran IC
CR1-mediated ATP release by human red blood cells promotes CR1 clustering and modulates the immune transfer process. J Biol Chem. 2013 Oct 25; 288(43):31139-53.
CR1-mediated ATP release by human red blood cells promotes CR1 clustering and modulates the immune transfer process.
Muroya T, Kannan L, Ghiran IC, Shevkoplyas SS, Paz Z, Tsokos M, Dalle Lucca JJ, Shapiro NI, Tsokos GC
C4d deposits on the surface of RBCs in trauma patients and interferes with their function. Crit Care Med. 2014 May; 42(5):e364-72.
C4d deposits on the surface of RBCs in trauma patients and interferes with their function.
Cannabinoids shift the basal ganglia microRNA m6A methylation profile towards an anti-inflammatory phenotype in SIV-infected rhesus macaques. Commun Biol. 2025 Nov 24; 8(1):1659.
Cannabinoids shift the basal ganglia microRNA m6A methylation profile towards an anti-inflammatory phenotype in SIV-infected rhesus macaques.
Naushad W, Okeoma BC, Gartner C, Santos-Ortega Y, Vary CPH, Premadasa LS, Noghero A, Stapleton JT, Ghiran IC, Mohan M, Okeoma CM
Non-Vesicular Extracellular Particle (NVEP) Proteomes from Diverse Biological Sources Reveal Specific Marker Composition with Varying Enrichment Levels. Biomolecules. 2025 10 22; 15(11).
Non-Vesicular Extracellular Particle (NVEP) Proteomes from Diverse Biological Sources Reveal Specific Marker Composition with Varying Enrichment Levels.
Jena BP, Larsson L, Gatti DL, Ghiran I, Cho WJ
Understanding Brain-Skeletal Muscle Crosstalk Impacting Metabolism and Movement. Discoveries (Craiova). 2022 Jan-Mar; 10(1):e144.
Understanding Brain-Skeletal Muscle Crosstalk Impacting Metabolism and Movement.
Willms AG, Wöhler A, Schwab R, Schaaf S, Slomka A, Ghiran I, Robson SC, Kornek MT
Editorial: Unveiling immune biomarkers: advancing trauma care through cellular and vesicular insights. Front Immunol. 2025; 16:1716977.
Editorial: Unveiling immune biomarkers: advancing trauma care through cellular and vesicular insights.
Qi Y, Li X, Song X, Wei W, Ghiran I, Robson SC
Purinergic signal transduction and metabolic regulation by ENTPD5 and ENTPD6. Front Endocrinol (Lausanne). 2026; 17:1680378.
Purinergic signal transduction and metabolic regulation by ENTPD5 and ENTPD6.
El Assal R, Guven S, Gurkan UA, Gozen I, Shafiee H, Dalbeyler S, Abdalla N, Thomas G, Fuld W, Illigens BM, Estanislau J, Khoory J, Kaufman R, Zylberberg C, Lindeman N, Wen Q, Ghiran I, Demirci U
Advanced materials (Deerfield Beach, Fla.)
Bio-inspired cryo-ink preserves red blood cell phenotype and function during nanoliter vitrification. Adv Mater. 2014 Sep 03; 26(33):5815-22.
Bio-inspired cryo-ink preserves red blood cell phenotype and function during nanoliter vitrification.
Tullius SG, Biefer HR, Li S, Trachtenberg AJ, Edtinger K, Quante M, Krenzien F, Uehara H, Yang X, Kissick HT, Kuo WP, Ghiran I, de la Fuente MA, Arredouani MS, Camacho V, Tigges JC, Toxavidis V, El Fatimy R, Smith BD, Vasudevan A, ElKhal A
NAD+ protects against EAE by regulating CD4+ T-cell differentiation. Nat Commun. 2014 Oct 07; 5:5101.
NAD+ protects against EAE by regulating CD4+ T-cell differentiation.
Kreimer S, Belov AM, Ghiran I, Murthy SK, Frank DA, Ivanov AR
Mass-spectrometry-based molecular characterization of extracellular vesicles: lipidomics and proteomics. J Proteome Res. 2015 Jun 05; 14(6):2367-84.
Mass-spectrometry-based molecular characterization of extracellular vesicles: lipidomics and proteomics.
Gokhin DS, Nowak RB, Khoory JA, Piedra Ade L, Ghiran IC, Fowler VM
Dynamic actin filaments control the mechanical behavior of the human red blood cell membrane. Mol Biol Cell. 2015 May 01; 26(9):1699-710.
Dynamic actin filaments control the mechanical behavior of the human red blood cell membrane.
Tasoglu S, Khoory JA, Tekin HC, Thomas C, Karnoub AE, Ghiran IC, Demirci U
Levitational Image Cytometry with Temporal Resolution. Adv Mater. 2015 Jul 08; 27(26):3901-8.
Levitational Image Cytometry with Temporal Resolution.
Cytometry: Levitational Image Cytometry with Temporal Resolution (Adv. Mater. 26/2015). Adv Mater. 2015 Jul; 27(26):3900.
Cytometry: Levitational Image Cytometry with Temporal Resolution (Adv. Mater. 26/2015).
Durmus NG, Tekin HC, Guven S, Sridhar K, Arslan Yildiz A, Calibasi G, Ghiran I, Davis RW, Steinmetz LM, Demirci U
Magnetic levitation of single cells. Proc Natl Acad Sci U S A. 2015 Jul 14; 112(28):E3661-8.
Knowlton S, Yu CH, Jain N, Ghiran IC, Tasoglu S
Smart-Phone Based Magnetic Levitation for Measuring Densities. PLoS One. 2015; 10(8):e0134400.
Smart-Phone Based Magnetic Levitation for Measuring Densities.
Quesenberry PJ, Aliotta J, Camussi G, Abdel-Mageed AB, Wen S, Goldberg L, Zhang HG, Tetta C, Franklin J, Coffey RJ, Danielson K, Subramanya V, Ghiran I, Das S, Chen CC, Pusic KM, Pusic AD, Chatterjee D, Kraig RP, Balaj L, Dooner M
Potential functional applications of extracellular vesicles: a report by the NIH Common Fund Extracellular RNA Communication Consortium. J Extracell Vesicles. 2015; 4:27575.
Potential functional applications of extracellular vesicles: a report by the NIH Common Fund Extracellular RNA Communication Consortium.
Knowlton SM, Sencan I, Aytar Y, Khoory J, Heeney MM, Ghiran IC, Tasoglu S
Sickle cell detection using a smartphone. Sci Rep. 2015 Oct 22; 5:15022.
Sickle cell detection using a smartphone.
Danielson KM, Estanislau J, Tigges J, Toxavidis V, Camacho V, Felton EJ, Khoory J, Kreimer S, Ivanov AR, Mantel PY, Jones J, Akuthota P, Das S, Ghiran I
Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry. PLoS One. 2016; 11(1):e0144678.
Diurnal Variations of Circulating Extracellular Vesicles Measured by Nano Flow Cytometry.
Khoory J, Estanislau J, Elkhal A, Lazaar A, Melhorn MI, Brodsky A, Illigens B, Hamachi I, Kurishita Y, Ivanov AR, Shevkoplyas S, Shapiro NI, Ghiran IC
Ligation of Glycophorin A Generates Reactive Oxygen Species Leading to Decreased Red Blood Cell Function. PLoS One. 2016; 11(1):e0141206.
Ligation of Glycophorin A Generates Reactive Oxygen Species Leading to Decreased Red Blood Cell Function.
Elkhal A, Rodriguez Cetina Biefer H, Heinbokel T, Uehara H, Quante M, Seyda M, Schuitenmaker JM, Krenzien F, Camacho V, de la Fuente MA, Ghiran I, Tullius SG
NAD(+) regulates Treg cell fate and promotes allograft survival via a systemic IL-10 production that is CD4(+) CD25(+) Foxp3(+) T cells independent. Sci Rep. 2016 Mar 01; 6:22325.
NAD(+) regulates Treg cell fate and promotes allograft survival via a systemic IL-10 production that is CD4(+) CD25(+) Foxp3(+) T cells independent.
Felton EJ, Velasquez A, Lu S, Murphy RO, ElKhal A, Mazor O, Gorelik P, Sharda A, Ghiran IC
Detection and quantification of subtle changes in red blood cell density using a cell phone. Lab Chip. 2016 08 16; 16(17):3286-95.
Detection and quantification of subtle changes in red blood cell density using a cell phone.
Malaria screening in resource-poor settings using a simple, power-free, cell phone-friendly device
Impact of circadian rhythm in obtaining reference profiles of exRNAs in healthy i
Modulation of erythrocyte function by complement
Neutrophil ecto-Calreticulin: Implications for Immunity
Mantel PY, Hjelmqvist D, Walch M, Kharoubi-Hess S, Nilsson S, Ravel D, Ribeiro M, Grüring C, Ma S, Padmanabhan P, Trachtenberg A, Ankarklev J, Brancucci NM, Huttenhower C, Duraisingh MT, Ghiran I, Kuo WP, Filgueira L, Martinelli R, Marti M
Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria. Nat Commun. 2016 10 10; 7:12727.
Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria.
Akuthota P, Carmo LA, Bonjour K, Murphy RO, Silva TP, Gamalier JP, Capron KL, Tigges J, Toxavidis V, Camacho V, Ghiran I, Ueki S, Weller PF, Melo RC
Extracellular Microvesicle Production by Human Eosinophils Activated by "Inflammatory" Stimuli. Front Cell Dev Biol. 2016; 4:117.
Extracellular Microvesicle Production by Human Eosinophils Activated by "Inflammatory" Stimuli.
Melo RC, Spencer LA, Perez SA, Ghiran I, Dvorak AM, Weller PF
Human eosinophils secrete preformed, granule-stored interleukin-4 through distinct vesicular compartments. Traffic. 2005 Nov; 6(11):1047-57.
Human eosinophils secrete preformed, granule-stored interleukin-4 through distinct vesicular compartments.
Bandeira-Melo C, Perez SA, Melo RC, Ghiran I, Weller PF
EliCell assay for the detection of released cytokines from eosinophils. J Immunol Methods. 2003 May 01; 276(1-2):227-37.
EliCell assay for the detection of released cytokines from eosinophils.
Pilsczek FH, Nicholson-Weller A, Ghiran I
Phagocytosis of Salmonella montevideo by human neutrophils: immune adherence increases phagocytosis, whereas the bacterial surface determines the route of intracellular processing. J Infect Dis. 2005 Jul 15; 192(2):200-9.
Phagocytosis of Salmonella montevideo by human neutrophils: immune adherence increases phagocytosis, whereas the bacterial surface determines the route of intracellular processing.
Ghiran I, Barbashov SF, Klickstein LB, Tas SW, Jensenius JC, Nicholson-Weller A
Complement receptor 1/CD35 is a receptor for mannan-binding lectin. J Exp Med. 2000 Dec 18; 192(12):1797-808.
Complement receptor 1/CD35 is a receptor for mannan-binding lectin.
Simonson B, Subramanya V, Chan MC, Zhang A, Franchino H, Ottaviano F, Mishra MK, Knight AC, Hunt D, Ghiran I, Khurana TS, Kontaridis MI, Rosenzweig A, Das S
DDiT4L promotes autophagy and inhibits pathological cardiac hypertrophy in response to stress. Sci Signal. 2017 02 28; 10(468).
DDiT4L promotes autophagy and inhibits pathological cardiac hypertrophy in response to stress.
Ghiran I, Tyagi SR, Klickstein LB, Nicholson-Weller A
Expression and function of C1q receptors and C1q binding proteins at the cell surface. Immunobiology. 2002 Sep; 205(4-5):407-20.
Expression and function of C1q receptors and C1q binding proteins at the cell surface.
Systemic lupus erythematosus serum deposits C4d on red blood cells, decreases red blood cell membrane deformability, and promotes nitric oxide production. Arthritis Rheum. 2011 Feb; 63(2):503-12.
Systemic lupus erythematosus serum deposits C4d on red blood cells, decreases red blood cell membrane deformability, and promotes nitric oxide production.
Ghiran I, Glodek AM, Weaver G, Klickstein LB, Nicholson-Weller A
Ligation of erythrocyte CR1 induces its clustering in complex with scaffolding protein FAP-1. Blood. 2008 Oct 15; 112(8):3465-73.
Ligation of erythrocyte CR1 induces its clustering in complex with scaffolding protein FAP-1.
Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury. Basic Res Cardiol. 2017 07; 112(4):38.
Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury.
Otabor I, Tyagi S, Beurskens FJ, Ghiran I, Schwab P, Nicholson-Weller A, Klickstein LB
A role for lipid rafts in C1q-triggered O2- generation by human neutrophils. Mol Immunol. 2004 Jun; 41(2-3):185-90.
A role for lipid rafts in C1q-triggered O2- generation by human neutrophils.
Borschukova O, Paz Z, Ghiran IC, Liu CC, Kao AH, Manzi S, Ahearn JM, Tsokos GC
Complement fragment C3d is colocalized within the lipid rafts of T cells and promotes cytokine production. Lupus. 2012 Oct; 21(12):1294-304.
Complement fragment C3d is colocalized within the lipid rafts of T cells and promotes cytokine production.
Costa M, Ghiran I, Peng CK, Nicholson-Weller A, Goldberger AL
Physical review. E, Statistical, nonlinear, and soft matter physics
Complex dynamics of human red blood cell flickering: alterations with in vivo aging. Phys Rev E Stat Nonlin Soft Matter Phys. 2008 Aug; 78(2 Pt 1):020901.
Complex dynamics of human red blood cell flickering: alterations with in vivo aging.
Journal of applied oral science : revista FOB
Examining the role of microvesicles to develop prognostic and diagnostic assays. J Appl Oral Sci. 2010 Sep-Oct; 18(5):i.
Examining the role of microvesicles to develop prognostic and diagnostic assays.
Ueki S, Melo RC, Ghiran I, Spencer LA, Dvorak AM, Weller PF
Eosinophil extracellular DNA trap cell death mediates lytic release of free secretion-competent eosinophil granules in humans. Blood. 2013 Mar 14; 121(11):2074-83.
Eosinophil extracellular DNA trap cell death mediates lytic release of free secretion-competent eosinophil granules in humans.
Kuo WP, Tigges JC, Toxavidis V, Ghiran I
Methods in molecular biology (Clifton, N.J.)
Red Blood Cells: A Source of Extracellular Vesicles. Methods Mol Biol. 2017; 1660:15-22.
Red Blood Cells: A Source of Extracellular Vesicles.
Andersen MS, Howard E, Lu S, Richard M, Gregory M, Ogembo G, Mazor O, Gorelik P, Shapiro NI, Sharda AV, Ghiran I
Detection of membrane-bound and soluble antigens by magnetic levitation. Lab Chip. 2017 10 11; 17(20):3462-3473.
Detection of membrane-bound and soluble antigens by magnetic levitation.
Shah R, Yeri A, Das A, Courtright-Lim A, Ziegler O, Gervino E, Ocel J, Quintero-Pinzon P, Wooster L, Bailey CS, Tanriverdi K, Beaulieu LM, Freedman JE, Ghiran I, Lewis GD, Van Keuren-Jensen K, Das S
American journal of physiology. Heart and circulatory physiology
Small RNA-seq during acute maximal exercise reveal RNAs involved in vascular inflammation and cardiometabolic health: brief report. Am J Physiol Heart Circ Physiol. 2017 Dec 01; 313(6):H1162-H1167.
Small RNA-seq during acute maximal exercise reveal RNAs involved in vascular inflammation and cardiometabolic health: brief report.
Itzkan I, Qiu L, Fang H, Zaman MM, Vitkin E, Ghiran IC, Salahuddin S, Modell M, Andersson C, Kimerer LM, Cipolloni PB, Lim KH, Freedman SD, Bigio I, Sachs BP, Hanlon EB, Perelman LT
Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels. Proc Natl Acad Sci U S A. 2007 Oct 30; 104(44):17255-60.
Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels.
Bandeira-Melo C, Gillard G, Ghiran I, Weller PF
EliCell: a gel-phase dual antibody capture and detection assay to measure cytokine release from eosinophils. J Immunol Methods. 2000 Oct 20; 244(1-2):105-15.
EliCell: a gel-phase dual antibody capture and detection assay to measure cytokine release from eosinophils.
Repik A, Pincus SE, Ghiran I, Nicholson-Weller A, Asher DR, Cerny AM, Casey LS, Jones SM, Jones SN, Mohamed N, Klickstein LB, Spitalny G, Finberg RW
A transgenic mouse model for studying the clearance of blood-borne pathogens via human complement receptor 1 (CR1). Clin Exp Immunol. 2005 May; 140(2):230-40.
A transgenic mouse model for studying the clearance of blood-borne pathogens via human complement receptor 1 (CR1).
Huang IC, Bosch BJ, Li F, Li W, Lee KH, Ghiran S, Vasilieva N, Dermody TS, Harrison SC, Dormitzer PR, Farzan M, Rottier PJ, Choe H
SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells. J Biol Chem. 2006 Feb 10; 281(6):3198-203.
SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells.
Introduction to fluorescence microscopy. Methods Mol Biol. 2011; 689:93-136.
Introduction to fluorescence microscopy.
Glodek AM, Mirchev R, Golan DE, Khoory JA, Burns JM, Shevkoplyas SS, Nicholson-Weller A, Ghiran IC
Ligation of complement receptor 1 increases erythrocyte membrane deformability. Blood. 2010 Dec 23; 116(26):6063-71.
Ligation of complement receptor 1 increases erythrocyte membrane deformability.
Babatunde KA, Mbagwu S, Hernández-Castañeda MA, Adapa SR, Walch M, Filgueira L, Falquet L, Jiang RHY, Ghiran I, Mantel PY
Malaria infected red blood cells release small regulatory RNAs through extracellular vesicles. Sci Rep. 2018 01 17; 8(1):884.
Malaria infected red blood cells release small regulatory RNAs through extracellular vesicles.
Rodriguez Cetina Biefer H, Heinbokel T, Uehara H, Camacho V, Minami K, Nian Y, Koduru S, El Fatimy R, Ghiran I, Trachtenberg AJ, de la Fuente MA, Azuma H, Akbari O, Tullius SG, Vasudevan A, Elkhal A
The Journal of allergy and clinical immunology
Mast cells regulate CD4+ T-cell differentiation in the absence of antigen presentation. J Allergy Clin Immunol. 2018 12; 142(6):1894-1908.e7.
Mast cells regulate CD4+ T-cell differentiation in the absence of antigen presentation.