Speer EM, Dowling DJ, Xu J, Ozog LS, Mathew JA, Chander A, Yin D, Levy O
Pentoxifylline, dexamethasone and azithromycin demonstrate distinct age-dependent and synergistic inhibition of TLR- and inflammasome-mediated cytokine production in human newborn and adult blood in vitro. PLoS One. 2018; 13(5):e0196352.
Pentoxifylline, dexamethasone and azithromycin demonstrate distinct age-dependent and synergistic inhibition of TLR- and inflammasome-mediated cytokine production in human newborn and adult blood in vitro.
Hamilton CM, Dowling DJ, Loscher CE, Morphew RM, Brophy PM, O'Neill SM
The Fasciola hepatica tegumental antigen suppresses dendritic cell maturation and function. Infect Immun. 2009 Jun; 77(6):2488-98.
The Fasciola hepatica tegumental antigen suppresses dendritic cell maturation and function.
LaCourse EJ, Perally S, Morphew RM, Moxon JV, Prescott M, Dowling DJ, O'Neill SM, Kipar A, Hetzel U, Hoey E, Zafra R, Buffoni L, Pérez Arévalo J, Brophy PM
The Sigma class glutathione transferase from the liver fluke Fasciola hepatica. PLoS Negl Trop Dis. 2012; 6(5):e1666.
The Sigma class glutathione transferase from the liver fluke Fasciola hepatica.
Speer EM, Diago-Navarro E, Ozog LS, Dowling DJ, Hou W, Raheel M, Fries BC, Levy O
Pentoxifylline Alone or in Combination with Gentamicin or Vancomycin Inhibits Live Microbe-Induced Proinflammatory Cytokine Production in Human Cord Blood and Cord Blood Monocytes In Vitro. Antimicrob Agents Chemother. 2018 12; 62(12).
Pentoxifylline Alone or in Combination with Gentamicin or Vancomycin Inhibits Live Microbe-Induced Proinflammatory Cytokine Production in Human Cord Blood and Cord Blood Monocytes In Vitro.
Queenan AM, Dowling DJ, Cheng WK, Faé K, Fernandez J, Flynn PJ, Joshi S, Brightman SE, Ramirez J, Serroyen J, Wiertsema S, Fortanier A, van den Dobbelsteen G, Levy O, Poolman J
Increasing FIM2/3 antigen-content improves efficacy of Bordetella pertussis vaccines in mice in vivo without altering vaccine-induced human reactogenicity biomarkers in vitro. Vaccine. 2019 01 03; 37(1):80-89.
Increasing FIM2/3 antigen-content improves efficacy of Bordetella pertussis vaccines in mice in vivo without altering vaccine-induced human reactogenicity biomarkers in vitro.
Philbin VJ, Dowling DJ, Gallington LC, Cortés G, Tan Z, Suter EE, Chi KW, Shuckett A, Stoler-Barak L, Tomai M, Miller RL, Mansfield K, Levy O
The Journal of allergy and clinical immunology
Imidazoquinoline Toll-like receptor 8 agonists activate human newborn monocytes and dendritic cells through adenosine-refractory and caspase-1-dependent pathways. J Allergy Clin Immunol. 2012 Jul; 130(1):195-204.e9.
Imidazoquinoline Toll-like receptor 8 agonists activate human newborn monocytes and dendritic cells through adenosine-refractory and caspase-1-dependent pathways.
Vukman KV, Adams PN, Dowling D, Metz M, Maurer M, O'Neill SM
The effects of Fasciola hepatica tegumental antigens on mast cell function. Int J Parasitol. 2013 Jun; 43(7):531-9.
The effects of Fasciola hepatica tegumental antigens on mast cell function.
Recent Advances in the Discovery and Delivery of TLR7/8 Agonists as Vaccine Adjuvants. Immunohorizons. 2018 07 02; 2(6):185-197.
Recent Advances in the Discovery and Delivery of TLR7/8 Agonists as Vaccine Adjuvants.
William Bosworth Castle Professor of Medicine
Elizabeth G. and Gary J. Nabel Family Professor Professor of Medicine
This project characterized mechanisms underlying BCG’s immunostimulation of neonatal immunity, including “trained” immunity, and applied these towards rational design of scalable, synthetic neonatal subunit vaccine formulations.
Leveraging immunostimulatory pathways that enhance BCG vaccination for the rational design of synthetic neonatal vaccine formulations
Toward precision adjuvants: optimizing science and safety. Curr Opin Pediatr. 2020 02; 32(1):125-138.
Toward precision adjuvants: optimizing science and safety.
The TLR5 Agonist Flagellin Shapes Phenotypical and Functional Activation of Lung Mucosal Antigen Presenting Cells in Neonatal Mice. Front Immunol. 2020; 11:171.
The TLR5 Agonist Flagellin Shapes Phenotypical and Functional Activation of Lung Mucosal Antigen Presenting Cells in Neonatal Mice.
Angelidou A, Diray-Arce J, Conti MG, Smolen KK, van Haren SD, Dowling DJ, Husson RN, Levy O
BCG as a Case Study for Precision Vaccine Development: Lessons From Vaccine Heterogeneity, Trained Immunity, and Immune Ontogeny. Front Microbiol. 2020; 11:332.
BCG as a Case Study for Precision Vaccine Development: Lessons From Vaccine Heterogeneity, Trained Immunity, and Immune Ontogeny.
This contract is to discover novel adjuvants for influenza and coronavirus vaccines by screening ~100,000 small molecules for activity towards human leukocytes, including newborn and elderly leukocytes, define mechanisms of action, and develop formulated analogs for in vivo murine and ferret studies.
Soni D, Bobbala S, Li S, Scott EA, Dowling DJ
The sixth revolution in pediatric vaccinology: immunoengineering and delivery systems. Pediatr Res. 2021 05; 89(6):1364-1372.
The sixth revolution in pediatric vaccinology: immunoengineering and delivery systems.
Sanchez-Schmitz G, Morrocchi E, Cooney M, Soni D, Khatun R, Palma P, Dowling DJ, Levy O
Neonatal monocytes demonstrate impaired homeostatic extravasation into a microphysiological human vascular model. Sci Rep. 2020 10 20; 10(1):17836.
Neonatal monocytes demonstrate impaired homeostatic extravasation into a microphysiological human vascular model.
Soni D, Van Haren SD, Idoko OT, Evans JT, Diray-Arce J, Dowling DJ, Levy O
Towards Precision Vaccines: Lessons From the Second International Precision Vaccines Conference. Front Immunol. 2020; 11:590373.
Towards Precision Vaccines: Lessons From the Second International Precision Vaccines Conference.
Nanishi E, Borriello F, O'Meara TR, McGrath ME, Saito Y, Haupt RE, Seo HS, van Haren SD, Brook B, Chen J, Diray-Arce J, Doss-Gollin S, Leon M, Chew K, Menon M, Song K, Xu AZ, Caradonna TM, Feldman J, Hauser BM, Schmidt AG, Sherman AC, Baden LR, Ernst RK, Dillen C, Weston SM, Johnson RM, Hammond HL, Mayer R, Burke A, Bottazzi ME, Hotez PJ, Strych U, Chang A, Yu J, Barouch DH, Dhe-Paganon S, Zanoni I, Ozonoff A, Frieman MB, Levy O, Dowling DJ
bioRxiv : the preprint server for biology
Alum:CpG adjuvant enables SARS-CoV-2 RBD-induced protection in aged mice and synergistic activation of human elder type 1 immunity. bioRxiv. 2021 May 21.
Alum:CpG adjuvant enables SARS-CoV-2 RBD-induced protection in aged mice and synergistic activation of human elder type 1 immunity.
Nanishi E, Borriello F, O'Meara TR, McGrath ME, Saito Y, Haupt RE, Seo HS, van Haren SD, Cavazzoni CB, Brook B, Barman S, Chen J, Diray-Arce J, Doss-Gollin S, De Leon M, Prevost-Reilly A, Chew K, Menon M, Song K, Xu AZ, Caradonna TM, Feldman J, Hauser BM, Schmidt AG, Sherman AC, Baden LR, Ernst RK, Dillen C, Weston SM, Johnson RM, Hammond HL, Mayer R, Burke A, Bottazzi ME, Hotez PJ, Strych U, Chang A, Yu J, Sage PT, Barouch DH, Dhe-Paganon S, Zanoni I, Ozonoff A, Frieman MB, Levy O, Dowling DJ
An aluminum hydroxide:CpG adjuvant enhances protection elicited by a SARS-CoV-2 receptor binding domain vaccine in aged mice. Sci Transl Med. 2022 Jan 26; 14(629):eabj5305.
An aluminum hydroxide:CpG adjuvant enhances protection elicited by a SARS-CoV-2 receptor binding domain vaccine in aged mice.
Borriello F, Poli V, Shrock E, Spreafico R, Liu X, Pishesha N, Carpenet C, Chou J, Di Gioia M, McGrath ME, Dillen CA, Barrett NA, Lacanfora L, Franco ME, Marongiu L, Iwakura Y, Pucci F, Kruppa MD, Ma Z, Lowman DW, Ensley HE, Nanishi E, Saito Y, O'Meara TR, Seo HS, Dhe-Paganon S, Dowling DJ, Frieman M, Elledge SJ, Levy O, Irvine DJ, Ploegh HL, Williams DL, Zanoni I
An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity. Cell. 2022 02 17; 185(4):614-629.e21.
An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity.
This contract is to develop an effective adjuvanted anti-fentanyl vaccine via the induction of anti-fentanyl antibodies that will block the movement of fentanyl from the bloodstream to the brain where it can suppress respiration and cause death. The production of vaccine formulation pilot lots will be completed under good laboratory practice (GLP) to support Investigational New Drug application (IND)-enabling activities in preparation for chemistry, manufacturing, and control (CMC) process-development and current Good Manufacturing Practice (cGMP) production grade formulation scaled up, to support animal and early-stage Phase I/II safety and immunogenicity human clinical trials in adults and/or youths with opioid use disorder (OUD).
This contract focuses on development of novel adjuvanted SARS-CoV-2 vaccines tailored for use in older humans.
This award advances the clinical development of patented adjuvants developed within the Dowling Laboratory.
This contract supplement supports the development of novel TLR7/8 agonist adjuvants for use in influenza and coronavirus vaccines.
Vaccine Adjuvant Discovery Program - SARS-CoV-2 Vaccine Development
Adjuvant optimization via structure-activity relationship studies
Development of Vaccines for the Treatment of Opioid Use Disorder
Advancement of TLR7/8 Adjuvant Development
Barman S, Soni D, Brook B, Nanishi E, Dowling DJ
Precision Vaccine Development: Cues From Natural Immunity. Front Immunol. 2021; 12:662218.
Precision Vaccine Development: Cues From Natural Immunity.
Nanishi E, Angelidou A, Rotman C, Dowling DJ, Levy O, Ozonoff A
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
Precision Vaccine Adjuvants for Older Adults: A Scoping Review. Clin Infect Dis. 2022 08 15; 75(Suppl 1):S72-S80.
Precision Vaccine Adjuvants for Older Adults: A Scoping Review.
A Precision Adjuvant Approach to Enhance Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Vaccines Optimized for Immunologically Distinct Vulnerable Populations. Clin Infect Dis. 2022 08 15; 75(Suppl 1):S30-S36.
A Precision Adjuvant Approach to Enhance Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Vaccines Optimized for Immunologically Distinct Vulnerable Populations.
Weitzman ER, Kossowsky J, Blakemore LM, Cox R, Dowling DJ, Levy O, Needles EW, Levy S
Acceptability of a Fentanyl Vaccine to Prevent Opioid Overdose and Need for Personalized Decision-Making. Clin Infect Dis. 2022 08 15; 75(Suppl 1):S98-S109.
Acceptability of a Fentanyl Vaccine to Prevent Opioid Overdose and Need for Personalized Decision-Making.
Chew K, Lee B, van Haren SD, Nanishi E, O'Meara T, Splaine JB, DeLeon M, Soni D, Seo HS, Dhe-Paganon S, Ozonoff A, Smith JA, Levy O, Dowling DJ
Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells. bioRxiv. 2022 Jul 11.
Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells.
Nanishi E, McGrath ME, O'Meara TR, Barman S, Yu J, Wan H, Dillen CA, Menon M, Seo HS, Song K, Xu AZ, Sebastian L, Brook B, Bosco AN, Borriello F, Ernst RK, Barouch DH, Dhe-Paganon S, Levy O, Frieman MB, Dowling DJ
mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant. Commun Biol. 2022 08 06; 5(1):790.
mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant.
Barman S, Borriello F, Brook B, Pietrasanta C, De Leon M, Sweitzer C, Menon M, van Haren SD, Soni D, Saito Y, Nanishi E, Yi S, Bobbala S, Levy O, Scott EA, Dowling DJ
Shaping Neonatal Immunization by Tuning the Delivery of Synergistic Adjuvants via Nanocarriers. ACS Chem Biol. 2022 09 16; 17(9):2559-2571.
Shaping Neonatal Immunization by Tuning the Delivery of Synergistic Adjuvants via Nanocarriers.
Dowling DJ, Barman S, Smith AJ, Borriello F, Chaney D, Brightman SE, Melhem G, Brook B, Menon M, Soni D, Schüller S, Siram K, Nanishi E, Bazin HG, Burkhart DJ, Levy O, Evans JT
Development of a TLR7/8 agonist adjuvant formulation to overcome early life hyporesponsiveness to DTaP vaccination. Sci Rep. 2022 10 18; 12(1):16860.
Development of a TLR7/8 agonist adjuvant formulation to overcome early life hyporesponsiveness to DTaP vaccination.
O'Meara TR, Nanishi E, McGrath ME, Barman S, Dong D, Dillen C, Menon M, Seo HS, Dhe-Paganon S, Ernst RK, Levy O, Frieman MB, Dowling DJ
Reduced SARS-CoV-2 mRNA vaccine immunogenicity and protection in mice with diet-induced obesity and insulin resistance. bioRxiv. 2022 Dec 07.
Reduced SARS-CoV-2 mRNA vaccine immunogenicity and protection in mice with diet-induced obesity and insulin resistance.
Brook B, Fatou B, Kumar Checkervarty A, Barman S, Sweitzer C, Bosco AN, Sherman AC, Baden LR, Morrocchi E, Sanchez-Schmitz G, Palma P, Nanishi E, O'Meara TR, McGrath ME, Frieman MB, Soni D, van Haren SD, Ozonoff A, Diray-Arce J, Steen H, Dowling DJ, Levy O
The mRNA vaccine BNT162b2 demonstrates impaired TH1 immunogenicity in human elders in vitro and aged mice in vivo. Res Sq. 2022 Dec 21.
The mRNA vaccine BNT162b2 demonstrates impaired TH1 immunogenicity in human elders in vitro and aged mice in vivo.
Nanishi E, Borriello F, Seo HS, O'Meara TR, McGrath ME, Saito Y, Chen J, Diray-Arce J, Song K, Xu AZ, Barman S, Menon M, Dong D, Caradonna TM, Feldman J, Hauser BM, Schmidt AG, Baden LR, Ernst RK, Dillen C, Yu J, Chang A, Hilgers L, Platenburg PP, Dhe-Paganon S, Barouch DH, Ozonoff A, Zanoni I, Frieman MB, Dowling DJ, Levy O
Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice. NPJ Vaccines. 2023 Feb 14; 8(1):18.
Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice.
Publisher Correction: Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice. NPJ Vaccines. 2023 Mar 03; 8(1):30.
Publisher Correction: Carbohydrate fatty acid monosulphate: oil-in-water adjuvant enhances SARS-CoV-2 RBD nanoparticle-induced immunogenicity and protection in mice.
Precision Vaccinology Approaches for the Development of Adjuvanted Vaccines Targeted to Distinct Vulnerable Populations. Pharmaceutics. 2023 Jun 19; 15(6).
Precision Vaccinology Approaches for the Development of Adjuvanted Vaccines Targeted to Distinct Vulnerable Populations.
Chew K, Lee B, Ozonoff A, Smith JA, Levy O, Dowling DJ, Van Haren S
A protocol for high-throughput screening for immunomodulatory compounds using human primary cells. STAR Protoc. 2023 09 15; 4(3):102405.
A protocol for high-throughput screening for immunomodulatory compounds using human primary cells.
Reduced SARS-CoV-2 mRNA vaccine immunogenicity and protection in mice with diet-induced obesity and insulin resistance. J Allergy Clin Immunol. 2023 11; 152(5):1107-1120.e6.
Doss-Gollin S, Thomas S, Brook B, Abedi K, Lebas C, Auderset F, Lugo-Rodriguez Y, Sanchez-Schmitz G, Dowling DJ, Levy O, van Haren SD
Human in vitro modeling of adjuvant formulations demonstrates enhancement of immune responses to SARS-CoV-2 antigen. NPJ Vaccines. 2023 Oct 26; 8(1):163.
Human in vitro modeling of adjuvant formulations demonstrates enhancement of immune responses to SARS-CoV-2 antigen.
Schüller SS, Barman S, Mendez-Giraldez R, Soni D, Daley J, Baden LR, Levy O, Dowling DJ
Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro. Commun Biol. 2024 06 08; 7(1):709.
Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro.
Soni D, Borriello F, Scott DA, Feru F, DeLeon M, Brightman SE, Cheng WK, Melhem G, Smith JA, Ramirez JC, Barman S, Cameron M, Kelly A, Walker K, Nanishi E, van Haren SD, Phan T, Qi Y, Kinsey R, Raczy MM, Ozonoff A, Pettengill MA, Hubbell JA, Fox CB, Dowling DJ, Levy O
From hit to vial: Precision discovery and development of an imidazopyrimidine TLR7/8 agonist adjuvant formulation. Sci Adv. 2024 07 05; 10(27):eadg3747.
From hit to vial: Precision discovery and development of an imidazopyrimidine TLR7/8 agonist adjuvant formulation.
Sherman ME, Michalski J, Das S, Yang H, Chandrasekaran L, O'Meara TR, Dowling DJ, Levy O, Barnoy S, Venkatesan M, Ernst RK
BECC-engineered live-attenuated Shigella vaccine candidates display reduced endotoxicity with robust immunogenicity in mice. Res Sq. 2024 Jun 11.
BECC-engineered live-attenuated Shigella vaccine candidates display reduced endotoxicity with robust immunogenicity in mice.
Weitzman ER, Pierce SE, Blakemore LM, Murdock A, Angelidou A, Dowling DJ, Levy O, Levy S
Need for strategic communications and stakeholder engagement to advance acceptability of an overdose preventing vaccine targeting fentanyl. Vaccine. 2024 10 24; 42(24):126082.
Need for strategic communications and stakeholder engagement to advance acceptability of an overdose preventing vaccine targeting fentanyl.
Brook B, Duval V, Barman S, Speciner L, Sweitzer C, Khanmohammed A, Menon M, Foster K, Ghosh P, Abedi K, Koster J, Nanishi E, Baden LR, Levy O, VanCott T, Micol R, Dowling DJ
Adjuvantation of a SARS-CoV-2 mRNA vaccine with controlled tissue-specific expression of an mRNA encoding IL-12p70. Sci Transl Med. 2024 07 24; 16(757):eadm8451.
Adjuvantation of a SARS-CoV-2 mRNA vaccine with controlled tissue-specific expression of an mRNA encoding IL-12p70.
Weitzman ER, Alegria M, Caplan A, Dowling D, Evans J, Fisher CE, Jordan A, Kossowsky J, Landau M, Larson H, Levy O, Levy S, Mnookin S, Reif S, Ross J, Sherman AC
Social complexity of a fentanyl vaccine to prevent opioid overdose conference proceedings: Radcliffe Institute for Advanced Study conference proceedings. Vaccine. 2025 01 12; 44:126324.
Social complexity of a fentanyl vaccine to prevent opioid overdose conference proceedings: Radcliffe Institute for Advanced Study conference proceedings.
Brook B, Checkervarty AK, Barman S, Sweitzer C, Bosco AN, Sherman AC, Baden LR, Morrocchi E, Sanchez-Schmitz G, Palma P, Nanishi E, O'Meara TR, McGrath ME, Frieman MB, Soni D, van Haren SD, Ozonoff A, Diray-Arce J, Steen H, Dowling DJ, Levy O
The BNT162b2 mRNA vaccine demonstrates reduced age-associated TH1 support in vitro and in vivo. iScience. 2024 Nov 15; 27(11):111055.
The BNT162b2 mRNA vaccine demonstrates reduced age-associated TH1 support in vitro and in vivo.
Goetz MJ, Park KS, Joshi M, Gottlieb AP, Dowling DJ, Mitragotri S
Journal of controlled release : official journal of the Controlled Release Society
An ionic liquid-based adjuvant for modulating cellular and humoral immune responses. J Control Release. 2024 Dec; 376:632-645.
An ionic liquid-based adjuvant for modulating cellular and humoral immune responses.
Koster JA, Prevost-Reilly A, Soni D, Kelly A, Walker K, Norton EB, Levy O, Haile CN, Dowling DJ
Protocol for measuring anti-fentanyl antibodies in mouse serum by enzyme-linked immunosorbent assay. STAR Protoc. 2024 12 20; 5(4):103402.
Protocol for measuring anti-fentanyl antibodies in mouse serum by enzyme-linked immunosorbent assay.
BECC-engineered live-attenuated Shigella vaccine candidates display reduced endotoxicity with robust immunogenicity in mice. Vaccine. 2025 03 19; 50:126779.
Angelidou A, Koster JA, Sherman AC, McLoughlin C, Lalwani P, Kelly A, Saeed A, McEnaney K, Baden LR, Brogna M, Weitzman ER, Levy S, Dowling DJ, Levy O
Product and trial design considerations on the path towards a vaccine to combat opioid overdose. NPJ Vaccines. 2025 Feb 19; 10(1):35.
Product and trial design considerations on the path towards a vaccine to combat opioid overdose.
Barman S, Kelly A, Dong D, Patel A, Buonopane MJ, Gonzales J, Janoschek B, Draghi A, Dowling DJ
Cytometry. Part A : the journal of the International Society for Analytical Cytology
OMIP-111: Immune-Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular Cytokines. Cytometry A. 2025 Apr; 107(4):221-225.
OMIP-111: Immune-Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular Cytokines.
Brook B, Goetz M, Duval V, Micol R, Dowling DJ
mRNA vaccines: miRNA-based controlled biodistribution and directed adjuvantation. Trends Immunol. 2025 May; 46(5):357-360.
mRNA vaccines: miRNA-based controlled biodistribution and directed adjuvantation.
Lee B, Dong D, Nanishi E, Awad JM, Abedi KZ, Saito Y, Borriello F, Barman S, Brook B, Kelly A, Menon M, Marques-Mourlet C, Gupta MV, Lister I, Oh C, Lyskawa K, Goetz M, Walker K, Cheng WK, Brightman SE, Sharma P, O'Meara TR, Chew K, Vieira D, Ryff K, Sweitzer C, Thomas S, van Haren SD, Pettengill M, Seo HS, Dhe-Paganon S, Zhang W, Levy O, Dowling DJ
Discovery of a small molecule TLR3 agonist adjuvant. Nat Commun. 2025 Dec 04; 17(1):364.
Discovery of a small molecule TLR3 agonist adjuvant.
Vílchez JI, Niehaus K, Dowling DN, González-López J, Manzanera M
Protection of Pepper Plants from Drought by Microbacterium sp. 3J1 by Modulation of the Plant's Glutamine and a-ketoglutarate Content: A Comparative Metabolomics Approach. Front Microbiol. 2018; 9:284.
Protection of Pepper Plants from Drought by Microbacterium sp. 3J1 by Modulation of the Plant's Glutamine and a-ketoglutarate Content: A Comparative Metabolomics Approach.
Martínez-Cuesta R, Conlon R, Wang M, Blanco-Romero E, Durán D, Redondo-Nieto M, Dowling D, Garrido-Sanz D, Martin M, Germaine K, Rivilla R
Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process. Front Microbiol. 2023; 14:1158130.
Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process.
Oh C, Jung HN, Park J, Awad JM, Jiang D, Dowling DJ, Im HJ
Nanomedicine and Spleen-Targeting Strategies for Precision Immunomodulation: Advances, Challenges, and Future Perspectives. ACS Nano. 2025 Jul 08; 19(26):23491-23516.
Nanomedicine and Spleen-Targeting Strategies for Precision Immunomodulation: Advances, Challenges, and Future Perspectives.
Journal of immunology (Baltimore, Md. : 1950)
Deep phenomics of PRR agonist activated human blood. J Immunol. 2025 Dec 01; 214(12):3294-3309.
Deep phenomics of PRR agonist activated human blood.
Rathore R, Dowling DN, Forristal PD, Spink J, Cotter PD, Bulgarelli D, Germaine KJ
Crop Establishment Practices Are a Driver of the Plant Microbiota in Winter Oilseed Rape (Brassica napus). Front Microbiol. 2017; 8:1489.
Crop Establishment Practices Are a Driver of the Plant Microbiota in Winter Oilseed Rape (Brassica napus).
Oteino N, Lally RD, Kiwanuka S, Lloyd A, Ryan D, Germaine KJ, Dowling DN
Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Front Microbiol. 2015; 6:745.
Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates.
Incorporating meningeal immunity into vaccine development. Nat Commun. 2025 Oct 09; 16(1):8981.
Incorporating meningeal immunity into vaccine development.
Palmer CD, Ninkovic J, Prokopowicz ZM, Mancuso CJ, Marin A, Andrianov AK, Dowling DJ, Levy O
The effect of stable macromolecular complexes of ionic polyphosphazene on HIV Gag antigen and on activation of human dendritic cells and presentation to T-cells. Biomaterials. 2014 Oct; 35(31):8876-8886.
The effect of stable macromolecular complexes of ionic polyphosphazene on HIV Gag antigen and on activation of human dendritic cells and presentation to T-cells.
Ontogeny of early life immunity. Trends Immunol. 2014 Jul; 35(7):299-310.
Ganapathi L, Van Haren S, Dowling DJ, Bergelson I, Shukla NM, Malladi SS, Balakrishna R, Tanji H, Ohto U, Shimizu T, David SA, Levy O
The Imidazoquinoline Toll-Like Receptor-7/8 Agonist Hybrid-2 Potently Induces Cytokine Production by Human Newborn and Adult Leukocytes. PLoS One. 2015; 10(8):e0134640.
The Imidazoquinoline Toll-Like Receptor-7/8 Agonist Hybrid-2 Potently Induces Cytokine Production by Human Newborn and Adult Leukocytes.
The Pediatric infectious disease journal
Pediatric Vaccine Adjuvants: Components of the Modern Vaccinologist's Toolbox. Pediatr Infect Dis J. 2015 Dec; 34(12):1395-8.
Pediatric Vaccine Adjuvants: Components of the Modern Vaccinologist's Toolbox.
Oh DY, Dowling DJ, Ahmed S, Choi H, Brightman S, Bergelson I, Berger ST, Sauld JF, Pettengill M, Kho AT, Pollack HJ, Steen H, Levy O
Adjuvant-induced Human Monocyte Secretome Profiles Reveal Adjuvant- and Age-specific Protein Signatures. Mol Cell Proteomics. 2016 06; 15(6):1877-94.
Adjuvant-induced Human Monocyte Secretome Profiles Reveal Adjuvant- and Age-specific Protein Signatures.
van Haren SD, Ganapathi L, Bergelson I, Dowling DJ, Banks M, Samuels RC, Reed SG, Marshall JD, Levy O
In vitro cytokine induction by TLR-activating vaccine adjuvants in human blood varies by age and adjuvant. Cytokine. 2016 07; 83:99-109.
In vitro cytokine induction by TLR-activating vaccine adjuvants in human blood varies by age and adjuvant.
Pettengill MA, van Haren SD, Li N, Dowling DJ, Bergelson I, Jans J, Ferwerda G, Levy O
Distinct TLR-mediated cytokine production and immunoglobulin secretion in human newborn naïve B cells. Innate Immun. 2016 08; 22(6):433-43.
Distinct TLR-mediated cytokine production and immunoglobulin secretion in human newborn naïve B cells.
Early life immune ontogeny - understanding how we build and sustain immunity to infection. Perspect Public Health. 2016 Jul; 136(4):205-7.
Early life immune ontogeny - understanding how we build and sustain immunity to infection.
van Haren SD, Dowling DJ, Foppen W, Christensen D, Andersen P, Reed SG, Hershberg RM, Baden LR, Levy O
Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization. J Immunol. 2016 12 01; 197(11):4413-4424.
Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization.
Dowling DJ, Sanders H, Cheng WK, Joshi S, Brightman S, Bergelson I, Pietrasanta C, van Haren SD, van Amsterdam S, Fernandez J, van den Dobbelsteen GP, Levy O
A Meningococcal Outer Membrane Vesicle Vaccine Incorporating Genetically Attenuated Endotoxin Dissociates Inflammation from Immunogenicity. Front Immunol. 2016; 7:562.
A Meningococcal Outer Membrane Vesicle Vaccine Incorporating Genetically Attenuated Endotoxin Dissociates Inflammation from Immunogenicity.
Speer EM, Dowling DJ, Ozog LS, Xu J, Yang J, Kennady G, Levy O
Pentoxifylline inhibits TLR- and inflammasome-mediated in vitro inflammatory cytokine production in human blood with greater efficacy and potency in newborns. Pediatr Res. 2017 May; 81(5):806-816.
Pentoxifylline inhibits TLR- and inflammasome-mediated in vitro inflammatory cytokine production in human blood with greater efficacy and potency in newborns.
Dowling DJ, van Haren SD, Scheid A, Bergelson I, Kim D, Mancuso CJ, Foppen W, Ozonoff A, Fresh L, Theriot TB, Lackner AA, Fichorova RN, Smirnov D, Vasilakos JP, Beaurline JM, Tomai MA, Midkiff CC, Alvarez X, Blanchard JL, Gilbert MH, Aye PP, Levy O
TLR7/8 adjuvant overcomes newborn hyporesponsiveness to pneumococcal conjugate vaccine at birth. JCI Insight. 2017 03 23; 2(6):e91020.
TLR7/8 adjuvant overcomes newborn hyporesponsiveness to pneumococcal conjugate vaccine at birth.
Dowling DJ, Scott EA, Scheid A, Bergelson I, Joshi S, Pietrasanta C, Brightman S, Sanchez-Schmitz G, Van Haren SD, Ninkovic J, Kats D, Guiducci C, de Titta A, Bonner DK, Hirosue S, Swartz MA, Hubbell JA, Levy O
Toll-like receptor 8 agonist nanoparticles mimic immunomodulating effects of the live BCG vaccine and enhance neonatal innate and adaptive immune responses. J Allergy Clin Immunol. 2017 Nov; 140(5):1339-1350.
Toll-like receptor 8 agonist nanoparticles mimic immunomodulating effects of the live BCG vaccine and enhance neonatal innate and adaptive immune responses.
Dowling DJ, Noone CM, Adams PN, Vukman KV, Molloy SF, Forde J, Asaolu S, O'Neill SM
Ascaris lumbricoides pseudocoelomic body fluid induces a partially activated dendritic cell phenotype with Th2 promoting ability in vivo. Int J Parasitol. 2011 Feb; 41(2):255-61.
Ascaris lumbricoides pseudocoelomic body fluid induces a partially activated dendritic cell phenotype with Th2 promoting ability in vivo.
Noone C, Parkinson M, Dowling DJ, Aldridge A, Kirwan P, Molloy SF, Asaolu SO, Holland C, O'Neill SM
Plasma cytokines, chemokines and cellular immune responses in pre-school Nigerian children infected with Plasmodium falciparum. Malar J. 2013 Jan 07; 12:5.
Plasma cytokines, chemokines and cellular immune responses in pre-school Nigerian children infected with Plasmodium falciparum.
A comparative analysis of cytokine responses, cell surface marker expression and MAPKs in DCs matured with LPS compared with a panel of TLR ligands. Cytokine. 2008 Mar; 41(3):254-62.
A comparative analysis of cytokine responses, cell surface marker expression and MAPKs in DCs matured with LPS compared with a panel of TLR ligands.
Dowling DJ, Tan Z, Prokopowicz ZM, Palmer CD, Matthews MA, Dietsch GN, Hershberg RM, Levy O
The ultra-potent and selective TLR8 agonist VTX-294 activates human newborn and adult leukocytes. PLoS One. 2013; 8(3):e58164.
The ultra-potent and selective TLR8 agonist VTX-294 activates human newborn and adult leukocytes.
Morphew RM, Hamilton CM, Wright HA, Dowling DJ, O'Neill SM, Brophy PM
Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40. Vet Parasitol. 2013 Jan 31; 191(3-4):379-85.
Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40.
Borriello F, Pietrasanta C, Lai JCY, Walsh LM, Sharma P, O'Driscoll DN, Ramirez J, Brightman S, Pugni L, Mosca F, Burkhart DJ, Dowling DJ, Levy O
Identification and Characterization of Stimulator of Interferon Genes As a Robust Adjuvant Target for Early Life Immunization. Front Immunol. 2017; 8:1772.
Identification and Characterization of Stimulator of Interferon Genes As a Robust Adjuvant Target for Early Life Immunization.
Dowling DJ, Hamilton CM, Donnelly S, La Course J, Brophy PM, Dalton J, O'Neill SM
Major secretory antigens of the helminth Fasciola hepatica activate a suppressive dendritic cell phenotype that attenuates Th17 cells but fails to activate Th2 immune responses. Infect Immun. 2010 Feb; 78(2):793-801.
Major secretory antigens of the helminth Fasciola hepatica activate a suppressive dendritic cell phenotype that attenuates Th17 cells but fails to activate Th2 immune responses.
Scheid A, Borriello F, Pietrasanta C, Christou H, Diray-Arce J, Pettengill MA, Joshi S, Li N, Bergelson I, Kollmann T, Dowling DJ, Levy O
Adjuvant Effect of Bacille Calmette-Guérin on Hepatitis B Vaccine Immunogenicity in the Preterm and Term Newborn. Front Immunol. 2018; 9:29.
Adjuvant Effect of Bacille Calmette-Guérin on Hepatitis B Vaccine Immunogenicity in the Preterm and Term Newborn.