JDM8JeffreyMacklisJeffrey Daniel Macklis, Dipl.ScNat, Dr.Sc.Tech, M.D.42.36258150000000-71.0670240000000075560Macklis, JeffreyDaniel617/495-5413Max and Anne Wien Professor of Stem Cell and Regenerative Biologycatalyst:advisorInMentoringCompletedStudentProjectcompleted student projectscatalyst:eCommonsLogineCommons LoginMentoring - Completed Student Projectprns:coAuthorOfcoauthor ofprns:endDateend dateFaculty Rankprns:fullNamefull nameprns:hasAuthorListauthor listprns:hasFacultyRankhas faculty rankprns:hasNetworkhas networkprns:hasPublicationVenuepublished inprns:informationResourceReferenceinformation resource referenceprns:isPrimaryPositionis primary positionprns:latitudelatitudeprns:longitudelongitudeprns:maxWeightmaximum weightprns:medlineTAjournal title abbreviationprns:meshDescriptorUIMeSH DescriptorUIprns:meshSemanticGroupNameMeSH semantic group nameprns:minWeightminimum weightprns:numberOfAuthorsnumber of authorsprns:numberOfConnectionsnumber of connectionsprns:numberOfPublicationsnumber of publicationsprns:personIdPerson IDprns:personInPrimaryPositionperson in primary positionprns:physicalNeighborOfphysical neighborprns:positionInDepartmentposition in departmentprns:positionInDivisionposition in divisionprns:predicateNodepredicate nodeprns:principalInvestigatorNameprincipal investigator nameprns:publicationDatepublication dateprns:similarTosimilar toprns:sortOrdersort orderprns:startDatestart dateprns:uniquenessWeightuniqueness weightprns:yearyearAcademic ArticleArticleDocumentbibo:pmidPubMed IdentifierAddressvivo:address1address line 1vivo:address2address line 2vivo:address3address line 3vivo:addressCitycityvivo:addressPostalCodepostal codevivo:addressStatestate or provincevivo:authorInAuthorshipselected publicationsvivo:authorRankauthor rank in publicationAuthorshipDepartmentDivisionGrantvivo:hasResearchArearesearch areasvivo:hasResearcherRoleresearch activitiesvivo:hrJobTitleHR job titleInformation Resourcevivo:linkedAuthorlinked authorvivo:linkedInformationResourcelinked information resourcevivo:mailingAddressmailing addressvivo:middleNamemiddle name or initialvivo:overviewoverviewvivo:personInPositionpositionsvivo:phoneNumberphonePositionvivo:positionInOrganizationposition in organizationvivo:preferredTitlepreferred titleResearcher Rolevivo:researcherRoleOfresearcher role ofvivo:roleContributesTocontributes tovivo:sponsorAwardIdsponsor award idrdf:predicatepredicaterdf:typetyperdfs:labellabelConceptAgentfoaf:firstNamefirst namefoaf:lastNamelast nameOrganizationPerson10719901Scharff C, Kirn JR, Grossman M, Macklis JD, Nottebohm FNeuronTargeted neuronal death affects neuronal replacement and vocal behavior in adult songbirds. Neuron. 2000 Feb; 25(2):481-92.Neuron2000-02-01T00:00:002000Targeted neuronal death affects neuronal replacement and vocal behavior in adult songbirds.10879536Magavi SS, Leavitt BR, Macklis JDNatureInduction of neurogenesis in the neocortex of adult mice. Nature. 2000 Jun 22; 405(6789):951-5.Nature2000-06-22T00:00:002000Induction of neurogenesis in the neocortex of adult mice.11750176Magavi SS, Macklis JDNeuropsychopharmacology : official publication of the American College of NeuropsychopharmacologyManipulation of neural precursors in situ: induction of neurogenesis in the neocortex of adult mice. Neuropsychopharmacology. 2001 Dec; 25(6):816-35.Neuropsychopharmacology2001-12-01T00:00:002001Manipulation of neural precursors in situ: induction of neurogenesis in the neocortex of adult mice.4014918Anderson P, Macklis J, Brown M, Ory DAnnals of internal medicineEosinophilic cerebrospinal fluid pleocytosis and cryptococcal meningitis. Ann Intern Med. 1985 Aug; 103(2):306-7.Ann Intern Med1985-08-01T00:00:001985Eosinophilic cerebrospinal fluid pleocytosis and cryptococcal meningitis.Authorship 1051008629507412Wuttke TV, Markopoulos F, Padmanabhan H, Wheeler AP, Murthy VN, Macklis JDNature neuroscienceDevelopmentally primed cortical neurons maintain fidelity of differentiation and establish appropriate functional connectivity after transplantation. Nat Neurosci. 2018 04; 21(4):517-529.Nat Neurosci2018-03-05T00:00:002018Developmentally primed cortical neurons maintain fidelity of differentiation and establish appropriate functional connectivity after transplantation.15534207Chen J, Magavi SS, Macklis JDProceedings of the National Academy of Sciences of the United States of AmericaNeurogenesis of corticospinal motor neurons extending spinal projections in adult mice. Proc Natl Acad Sci U S A. 2004 Nov 16; 101(46):16357-62.Proc Natl Acad Sci U S A2004-11-08T00:00:002004Neurogenesis of corticospinal motor neurons extending spinal projections in adult mice.3859483Macklis JD, Sidman RL, Shine HDIn vitro cellular & developmental biology : journal of the Tissue Culture AssociationCross-linked collagen surface for cell culture that is stable, uniform, and optically superior to conventional surfaces. In Vitro Cell Dev Biol. 1985 Mar; 21(3 Pt 1):189-94.In Vitro Cell Dev Biol1985-03-01T00:00:001985Cross-linked collagen surface for cell culture that is stable, uniform, and optically superior to conventional surfaces.9878177Wang Y, Sheen VL, Macklis JDExperimental neurologyCortical interneurons upregulate neurotrophins in vivo in response to targeted apoptotic degeneration of neighboring pyramidal neurons. Exp Neurol. 1998 Dec; 154(2):389-402.Exp Neurol1998-12-01T00:00:001998Cortical interneurons upregulate neurotrophins in vivo in response to targeted apoptotic degeneration of neighboring pyramidal neurons.1912455Macklis JD, Quattrochi JJNeuroreportRestricted diffusion and stability of carbachol-fluorescent nanospheres in-vivo. Neuroreport. 1991 May; 2(5):247-50.Neuroreport1991-05-01T00:00:001991Restricted diffusion and stability of carbachol-fluorescent nanospheres in-vivo.18957218Joshi PS, Molyneaux BJ, Feng L, Xie X, Macklis JD, Gan LNeuronBhlhb5 regulates the postmitotic acquisition of area identities in layers II-V of the developing neocortex. Neuron. 2008 Oct 23; 60(2):258-72.Neuron2008-10-23T00:00:002008Bhlhb5 regulates the postmitotic acquisition of area identities in layers II-V of the developing neocortex.19657336Azim E, Jabaudon D, Fame RM, Macklis JDNature neuroscienceSOX6 controls dorsal progenitor identity and interneuron diversity during neocortical development. Nat Neurosci. 2009 Oct; 12(10):1238-47.Nat Neurosci2009-08-05T00:00:002009SOX6 controls dorsal progenitor identity and interneuron diversity during neocortical development.20133588Tomassy GS, De Leonibus E, Jabaudon D, Lodato S, Alfano C, Mele A, Macklis JD, Studer MProceedings of the National Academy of Sciences of the United States of AmericaArea-specific temporal control of corticospinal motor neuron differentiation by COUP-TFI. Proc Natl Acad Sci U S A. 2010 Feb 23; 107(8):3576-81.Proc Natl Acad Sci U S A2010-02-02T00:00:002010Area-specific temporal control of corticospinal motor neuron differentiation by COUP-TFI.743887Macklis JD, Ketterer FDCryobiologyMicrowave properties of cryoprotectants. Cryobiology. 1978 Dec; 15(6):627-35.Cryobiology1978-12-01T00:00:001978Microwave properties of cryoprotectants.Authorship 102661321411657Ozdinler PH, Benn S, Yamamoto TH, Güzel M, Brown RH, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceCorticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G?³A transgenic ALS mice. J Neurosci. 2011 Mar 16; 31(11):4166-77.J Neurosci2011-03-16T00:00:002011Corticospinal motor neurons and related subcerebral projection neurons undergo early and specific neurodegeneration in hSOD1G?³A transgenic ALS mice.18199763Arlotta P, Molyneaux BJ, Jabaudon D, Yoshida Y, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceCtip2 controls the differentiation of medium spiny neurons and the establishment of the cellular architecture of the striatum. J Neurosci. 2008 Jan 16; 28(3):622-32.J Neurosci2008-01-16T00:00:002008Ctip2 controls the differentiation of medium spiny neurons and the establishment of the cellular architecture of the striatum.11698598Catapano LA, Arnold MW, Perez FA, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceSpecific neurotrophic factors support the survival of cortical projection neurons at distinct stages of development. J Neurosci. 2001 Nov 15; 21(22):8863-72.J Neurosci2001-11-15T00:00:002001Specific neurotrophic factors support the survival of cortical projection neurons at distinct stages of development.11951629Magavi SS, Macklis JDMethods in molecular biology (Clifton, N.J.)Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo. Methods Mol Biol. 2002; 198:283-90.Methods Mol Biol2002-01-01T00:00:002002Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo.21129791Fame RM, MacDonald JL, Macklis JDTrends in neurosciencesDevelopment, specification, and diversity of callosal projection neurons. Trends Neurosci. 2011 Jan; 34(1):41-50.Trends Neurosci2010-12-02T00:00:002010Development, specification, and diversity of callosal projection neurons.18371403Breunig JJ, Arellano JI, Macklis JD, Rakic PCell stem cellEverything that glitters isn't gold: a critical review of postnatal neural precursor analyses. Cell Stem Cell. 2007 Dec 13; 1(6):612-27.Cell Stem Cell2007-12-13T00:00:002007Everything that glitters isn't gold: a critical review of postnatal neural precursor analyses.21799210Jabaudon D, Shnider SJ, Tischfield DJ, Galazo MJ, Macklis JDCerebral cortex (New York, N.Y. : 1991)RORß induces barrel-like neuronal clusters in the developing neocortex. Cereb Cortex. 2012 May; 22(5):996-1006.Cereb Cortex2011-07-28T00:00:002011RORß induces barrel-like neuronal clusters in the developing neocortex.22116886Czupryn A, Zhou YD, Chen X, McNay D, Anderson MP, Flier JS, Macklis JDScience (New York, N.Y.)Transplanted hypothalamic neurons restore leptin signaling and ameliorate obesity in db/db mice. Science. 2011 Nov 25; 334(6059):1133-7.Science2011-11-25T00:00:002011Transplanted hypothalamic neurons restore leptin signaling and ameliorate obesity in db/db mice.22347158Sohur US, Arlotta P, Macklis JDFrontiers in neuroscienceDevelopmental Controls are Re-Expressed during Induction of Neurogenesis in the Neocortex of Young Adult Mice. Front Neurosci. 2012; 6:12.Front Neurosci2012-02-06T00:00:002012Developmental Controls are Re-Expressed during Induction of Neurogenesis in the Neocortex of Young Adult Mice.23034677Tharin S, Kothapalli CR, Ozdinler PH, Pasquina L, Chung S, Varner J, DeValence S, Kamm R, Macklis JDIntegrative biology : quantitative biosciences from nano to macroA microfluidic device to investigate axon targeting by limited numbers of purified cortical projection neuron subtypes. Integr Biol (Camb). 2012 Nov; 4(11):1398-405.Integr Biol (Camb)2012-11-01T00:00:002012A microfluidic device to investigate axon targeting by limited numbers of purified cortical projection neuron subtypes.15128396Catapano LA, Arlotta P, Cage TA, Macklis JDThe European journal of neuroscienceStage-specific and opposing roles of BDNF, NT-3 and bFGF in differentiation of purified callosal projection neurons toward cellular repair of complex circuitry. Eur J Neurosci. 2004 May; 19(9):2421-34.Eur J Neurosci2004-05-01T00:00:002004Stage-specific and opposing roles of BDNF, NT-3 and bFGF in differentiation of purified callosal projection neurons toward cellular repair of complex circuitry.Authorship 13391433365549Madison RD, Macklis JD, Frosch MPBrain researchNon-invasive laser microsurgery selectively damages populations of labeled mouse neurons: dependence on incident laser dose and absorption. Brain Res. 1988 Mar 29; 445(1):101-10.Brain Res1988-03-29T00:00:001988Non-invasive laser microsurgery selectively damages populations of labeled mouse neurons: dependence on incident laser dose and absorption.9326667Snyder EY, Yoon C, Flax JD, Macklis JDProceedings of the National Academy of Sciences of the United States of AmericaMultipotent neural precursors can differentiate toward replacement of neurons undergoing targeted apoptotic degeneration in adult mouse neocortex. Proc Natl Acad Sci U S A. 1997 Oct 14; 94(21):11663-8.Proc Natl Acad Sci U S A1997-10-14T00:00:001997Multipotent neural precursors can differentiate toward replacement of neurons undergoing targeted apoptotic degeneration in adult mouse neocortex.3103444Brasier AR, Macklis JD, Vaughan D, Warner L, Kirshenbaum JMThe American journal of medicineMyopericarditis as an initial presentation of meningococcemia. Unusual manifestation of infection with serotype W135. Am J Med. 1987 Mar 23; 82(3 Spec No):641-4.Am J Med1987-03-23T00:00:001987Myopericarditis as an initial presentation of meningococcemia. Unusual manifestation of infection with serotype W135.12846990Arlotta P, Magavi SS, Macklis JDAnnals of the New York Academy of SciencesInduction of adult neurogenesis: molecular manipulation of neural precursors in situ. Ann N Y Acad Sci. 2003 Jun; 991:229-36.Ann N Y Acad Sci2003-06-01T00:00:002003Induction of adult neurogenesis: molecular manipulation of neural precursors in situ.14980806Mellough CB, Cui Q, Spalding KL, Symons NA, Pollett MA, Snyder EY, Macklis JD, Harvey ARExperimental neurologyFate of multipotent neural precursor cells transplanted into mouse retina selectively depleted of retinal ganglion cells. Exp Neurol. 2004 Mar; 186(1):6-19.Exp Neurol2004-03-01T00:00:002004Fate of multipotent neural precursor cells transplanted into mouse retina selectively depleted of retinal ganglion cells.8339767Madison RD, Macklis JDExperimental neurologyNoninvasively induced degeneration of neocortical pyramidal neurons in vivo: selective targeting by laser activation of retrogradely transported photolytic chromophore. Exp Neurol. 1993 Jun; 121(2):153-9.Exp Neurol1993-06-01T00:00:001993Noninvasively induced degeneration of neocortical pyramidal neurons in vivo: selective targeting by laser activation of retrogradely transported photolytic chromophore.18369768Magavi SS, Macklis JDMethods in molecular biology (Clifton, N.J.)Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo. Methods Mol Biol. 2008; 438:335-43.Methods Mol Biol2008-01-01T00:00:002008Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo.11951631Magavi SS, Macklis JDMethods in molecular biology (Clifton, N.J.)Immunocytochemical analysis of neuronal differentiation. Methods Mol Biol. 2002; 198:291-7.Methods Mol Biol2002-01-01T00:00:002002Immunocytochemical analysis of neuronal differentiation.Authorship 1124174730626971Poulopoulos A, Murphy AJ, Ozkan A, Davis P, Hatch J, Kirchner R, Macklis JDNatureSubcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex. Nature. 2019 01; 565(7739):356-360.Nature2019-01-09T00:00:002019Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex.18494249Molyneaux BJ, Arlotta P, Macklis JDNovartis Foundation symposiumMolecular development of corticospinal motor neuron circuitry. Novartis Found Symp. 2007; 288:3-15; discussion 15-20, 96-8.Novartis Found Symp2007-01-01T00:00:002007Molecular development of corticospinal motor neuron circuitry.2475910Quattrochi JJ, Mamelak AN, Madison RD, Macklis JD, Hobson JAScience (New York, N.Y.)Mapping neuronal inputs to REM sleep induction sites with carbachol-fluorescent microspheres. Science. 1989 Sep 01; 245(4921):984-6.Science1989-09-01T00:00:001989Mapping neuronal inputs to REM sleep induction sites with carbachol-fluorescent microspheres.22634209Emsley JG, Menezes JR, Madeiro Da Costa RF, Martinez AM, Macklis JDExperimental neurologyIdentification of radial glia-like cells in the adult mouse olfactory bulb. Exp Neurol. 2012 Aug; 236(2):283-97.Exp Neurol2012-05-23T00:00:002012Identification of radial glia-like cells in the adult mouse olfactory bulb.477370Macklis JD, Ketterer FD, Cravalho EGCryobiologyTemperature dependence of the microwave properties of aqueous solutions of ethylene glycol between +15 degrees C and -70 degrees C. Cryobiology. 1979 Jun; 16(3):272-86.Cryobiology1979-06-01T00:00:001979Temperature dependence of the microwave properties of aqueous solutions of ethylene glycol between +15 degrees C and -70 degrees C.D011712Anatomy1502570.830632Pyramidal TractsD003337Anatomy3747390.684254Corpus CallosumD009474Anatomy222293360.24715NeuronsD019579Anatomy2124010.728781NeocortexD001369Anatomy66516890.540554AxonsDermatologyMedicineNeurobiologyNeurologyPediatricsStem Cell and Regenerative BiologyStem Cell and Regen BioGeneticsEndocrinologyBeth Israel Deaconess Medical CenterBrigham and Women's HospitalBoston Children's HospitalFaculty of Arts & SciencesHarvard Medical SchoolMassachusetts General HospitalHTK1HenryKeutmannHenry Todd Keutmann, M.D.42.36258150000000-71.0670240000000067900Keutmann, HenryAssociate Professor of Medicine (Biochemistry)11126selected publications5.578540.00274674458research areas2.106430.010936425coauthor of86.216819.626960similar toSIF1ShelleyFriedShelley Irwin Fried, Ph.D.42.36258150000000-71.067024000000007939Fried, ShelleyAssociate Professor of NeurosurgeryCW11ChristopherWalshChristopher A. Walsh, M.D., Ph.D.42.33849900000000-71.104460000000009592Walsh, ChristopherBullard Professor of Pediatrics and NeurologySY33Seok-HyunYunSeok-Hyun Yun, Ph.D.42.36258150000000-71.0670240000000087345Yun, Seok-HyunProfessor of Dermatology2018-12-28T00:00:002018-02-02T00:00:00Overcoming inhibition of axonal regeneration following spinal cord injury using subcellular RNA-proteome mapping in corticospinal neuron growth conesSummerUSS2UsharbudhSohurUsharbudh Shivraj Sohur, M.D.,Ph.D.42.36241700000000-71.0689370000000072106Sohur, UsharbudhInstructor in Neurology, Part-timeEWY2ElaineYuElaine Wei-Yin Yu, M.D.42.36258150000000-71.0670240000000022974Yu, ElaineAssociate Professor of MedicineBTH2BradleyHymanBradley Theodore Hyman, M.D., Ph.D.42.37904200000000-71.051481000000004393Hyman, BradleyJohn B. Penney, Jr. Professor of NeurologyBJM6BradleyMolyneauxBradley John Molyneaux, M.D., M.D.,Ph.D.42.38032740000000-71.1389101000000050602Molyneaux, BradleyMember of the Faculty of NeurologyMEG4MichaelGreenbergMichael Eldon Greenberg, Ph.D.42.33602040000000-71.1034325000000080338Greenberg, MichaelNathan Marsh Pusey Professor of NeurobiologyAuthorship 1370141VLS2VolneySheenVolney L Sheen, M.D., Ph.D.42.33837200000000-71.1032690000000010917Sheen, VolneyAssociate Professor of NeurologyMM74MichaelMannstadtMichael Mannstadt, M.D.42.36258150000000-71.0670240000000038064Mannstadt, MichaelAssociate Professor of MedicinePA5PaolaArlottaPaola Arlotta, Ph.D.42.36125700000000-71.0655270000000010833Arlotta, PaolaProfessor of Stem Cell and Regenerative Biologytrue1Associate Professor of NeurosurgeryAssociate Professor of Neurosurgerytrue1Associate Professor of NeurologyAssociate Professor of Neurologytrue1Associate Professor of Medicine (Biochemistry)Associate Professor of Medicine (Biochemistry)true1John B. Penney, Jr. Professor of NeurologyJohn B. Penney, Jr. Professor of Neurologytrue1Instructor in Neurology, Part-timeInstructor in Neurology, Part-timetrue1Associate Professor of MedicineAssociate Professor of Medicinetrue1Professor of DermatologyProfessor of DermatologyAuthorship 14684862025-01-31MACKLIS, JEFFREY D2020-03-15Molecular development and diversity of callosal projection neuronsR01NS104055Principal InvestigatorAuthorship 1682273Authorship 1246902432393583Ribeiro MC, Moore SM, Kishi N, Macklis JD, MacDonald JLeNeuroVitamin D Supplementation Rescues Aberrant NF-?B Pathway Activation and Partially Ameliorates Rett Syndrome Phenotypes in Mecp2 Mutant Mice. eNeuro. 2020 May/Jun; 7(3).eNeuro2020-05-22T00:00:002020Vitamin D Supplementation Rescues Aberrant NF-?B Pathway Activation and Partially Ameliorates Rett Syndrome Phenotypes in Mecp2 Mutant Mice.Authorship 1508333Authorship 1458443jas245JanetSongJanet Song, Ph.D.193281Song, JanetResearch Fellow in Pediatricstrue1Professor of NeurobiologyProfessor of NeurobiologyAuthorship 13061291433139574Diaz JL, Siththanandan VB, Lu V, Gonzalez-Nava N, Pasquina L, MacDonald JL, Woodworth MB, Ozkan A, Nair R, He Z, Sahni V, Sarnow P, Palmer TD, Macklis JD, Tharin SProceedings of the National Academy of Sciences of the United States of AmericaAn evolutionarily acquired microRNA shapes development of mammalian cortical projections. Proc Natl Acad Sci U S A. 2020 11 17; 117(46):29113-29122.Proc Natl Acad Sci U S A2020-11-02T00:00:002020An evolutionarily acquired microRNA shapes development of mammalian cortical projections.Authorship 1621475Authorship 2512035Authorship 1390567634473715Sadegh C, Ebina W, Arvanites AC, Davidow LS, Rubin LL, Macklis JDPloS oneSynthetic modified Fezf2 mRNA (modRNA) with concurrent small molecule SIRT1 inhibition enhances refinement of cortical subcerebral/corticospinal neuron identity from mouse embryonic stem cells. PLoS One. 2021; 16(9):e0254113.PLoS One2021-09-02T00:00:002021Synthetic modified Fezf2 mRNA (modRNA) with concurrent small molecule SIRT1 inhibition enhances refinement of cortical subcerebral/corticospinal neuron identity from mouse embryonic stem cells.Authorship 14042714Authorship 1404269734686320Sahni V, Shnider SJ, Jabaudon D, Song JHT, Itoh Y, Greig LC, Macklis JDCell reportsCorticospinal neuron subpopulation-specific developmental genes prospectively indicate mature segmentally specific axon projection targeting. Cell Rep. 2021 10 19; 37(3):109843.Cell Rep2021-10-19T00:00:002021Corticospinal neuron subpopulation-specific developmental genes prospectively indicate mature segmentally specific axon projection targeting.34686337Sahni V, Itoh Y, Shnider SJ, Macklis JDCell reportsCrim1 and Kelch-like 14 exert complementary dual-directional developmental control over segmentally specific corticospinal axon projection targeting. Cell Rep. 2021 10 19; 37(3):109842.Cell Rep2021-10-19T00:00:002021Crim1 and Kelch-like 14 exert complementary dual-directional developmental control over segmentally specific corticospinal axon projection targeting.Authorship 198465635022617Engmann AK, Hatch JJ, Nanda P, Veeraraghavan P, Ozkan A, Poulopoulos A, Murphy AJ, Macklis JDNature protocolsNeuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes. Nat Protoc. 2022 02; 17(2):222-251.Nat Protoc2022-01-12T00:00:002022Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.Authorship 14259078Authorship 2181033true1Associate Professor of MedicineAssociate Professor of Medicinetrue1Member of the Faculty of NeurologyMember of the Faculty of NeurologyAuthorship 4553703Authorship 15272045Authorship 1527288536823038Song JHT, Ruven C, Patel P, Ding F, Macklis JD, Sahni VThe Journal of neuroscience : the official journal of the Society for NeuroscienceCbln1 Directs Axon Targeting by Corticospinal Neurons Specifically toward Thoraco-Lumbar Spinal Cord. J Neurosci. 2023 03 15; 43(11):1871-1887.J Neurosci2023-02-23T00:00:002023Cbln1 Directs Axon Targeting by Corticospinal Neurons Specifically toward Thoraco-Lumbar Spinal Cord.36824714Greig LC, Woodworth MB, Poulopoulos A, Lim S, Macklis JDbioRxiv : the preprint server for biologyBEAM: a combinatorial recombinase toolbox for binary gene expression and mosaic analysis. bioRxiv. 2023 Feb 16.bioRxiv2023-02-16T00:00:002023BEAM: a combinatorial recombinase toolbox for binary gene expression and mosaic analysis.Authorship 1533698536934325Itoh Y, Sahni V, Shnider SJ, McKee H, Macklis JDCell reportsInter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations. Cell Rep. 2023 03 28; 42(3):112182.Cell Rep2023-03-17T00:00:002023Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations.Authorship 4887174Authorship 15842513Authorship 15851293Authorship 1585625337561632Galazo MJ, Sweetser DA, Macklis JDCell reportsTle4 controls both developmental acquisition and early post-natal maturation of corticothalamic projection neuron identity. Cell Rep. 2023 08 29; 42(8):112957.Cell Rep2023-08-09T00:00:002023Tle4 controls both developmental acquisition and early post-natal maturation of corticothalamic projection neuron identity.37609140Peter M, Shipman S, Macklis JDbioRxiv : the preprint server for biologyLimitations of fluorescent timer protein maturation kinetics to isolate transcriptionally synchronized cortically differentiating human pluripotent stem cells. bioRxiv. 2023 Aug 26.bioRxiv2023-08-26T00:00:002023Limitations of fluorescent timer protein maturation kinetics to isolate transcriptionally synchronized cortically differentiating human pluripotent stem cells.37624698Froberg JE, Durak O, Macklis JDCell reportsDevelopment of nanoRibo-seq enables study of regulated translation by cortical neuron subtypes, showing uORF translation in synaptic-axonal genes. Cell Rep. 2023 09 26; 42(9):112995.Cell Rep2023-08-24T00:00:002023Development of nanoRibo-seq enables study of regulated translation by cortical neuron subtypes, showing uORF translation in synaptic-axonal genes.Authorship 4578607Authorship 1779013Authorship 46933712026-08-31MACKLIS, JEFFREY D2023-09-01Subcellular mechanisms of subtype-specific neuron vulnerability in ALS and FTD: dysregulation of synapse-localized RNA, protein, and translation in mouse models and human cortico-spinal assembloidsRF1AG083085Principal Investigator3Professor of NeurologyProfessor of Neurology2Max and Anne Wien Professor of Stem Cell and Regenerative Biology and Professor of Surgery and Neurology, Harvard Medical SchoolMax and Anne Wien Professor of Stem Cell and Regenerative Biology and Professor of Surgery and Neurology, Harvard Medical Schooltrue1Professor of Stem Cell and Regenerative BiologyProfessor of Stem Cell and Regenerative Biologytrue1Max and Anne Wien Professor of Stem Cell and Regenerative BiologyMax and Anne Wien Professor of Stem Cell and Regenerative BiologyAuthorship 1628002738328182Veeraraghavan P, Engmann AK, Hatch JJ, Itoh Y, Nguyen D, Addison T, Macklis JDbioRxiv : the preprint server for biologyDynamic subtype- and context-specific subcellular RNA regulation in growth cones of developing neurons of the cerebral cortex. bioRxiv. 2024 Jan 23.bioRxiv2024-01-23T00:00:002024Dynamic subtype- and context-specific subcellular RNA regulation in growth cones of developing neurons of the cerebral cortex.true1Bullard Professor of Pediatrics and NeurologyBullard Professor of Pediatrics and Neurologytrue1Research Fellow in PediatricsResearch Fellow in Pediatricstrue1Nathan Marsh Pusey Professor of NeurobiologyNathan Marsh Pusey Professor of NeurobiologyAuthorship 3639851Authorship 2760045Authorship 6548221023678876Ravits J, Appel S, Baloh RH, Barohn R, Brooks BR, Elman L, Floeter MK, Henderson C, Lomen-Hoerth C, Macklis JD, McCluskey L, Mitsumoto H, Przedborski S, Rothstein J, Trojanowski JQ, van den Berg LH, Ringel SAmyotrophic lateral sclerosis & frontotemporal degenerationDeciphering amyotrophic lateral sclerosis: what phenotype, neuropathology and genetics are telling us about pathogenesis. Amyotroph Lateral Scler Frontotemporal Degener. 2013 May; 14 Suppl 1:5-18.Amyotroph Lateral Scler Frontotemporal Degener2013-05-01T00:00:002013Deciphering amyotrophic lateral sclerosis: what phenotype, neuropathology and genetics are telling us about pathogenesis.Authorship 5578862Authorship 1838387Authorship 1984134Authorship 680807524105342Greig LC, Woodworth MB, Galazo MJ, Padmanabhan H, Macklis JDNature reviews. NeuroscienceMolecular logic of neocortical projection neuron specification, development and diversity. Nat Rev Neurosci. 2013 11; 14(11):755-69.Nat Rev Neurosci2013-10-09T00:00:002013Molecular logic of neocortical projection neuron specification, development and diversity.Authorship 2772705Authorship 3155353NeurosurgeryAuthorship 2006153Authorship 2404134Authorship 704919523118198Sohur US, Padmanabhan HK, Kotchetkov IS, Menezes JR, Macklis JDCerebral cortex (New York, N.Y. : 1991)Anatomic and molecular development of corticostriatal projection neurons in mice. Cereb Cortex. 2014 Feb; 24(2):293-303.Cereb Cortex2012-10-31T00:00:002012Anatomic and molecular development of corticostriatal projection neurons in mice.Authorship 2457985Authorship 2476231Authorship 2809622Authorship 7361242Authorship 736584224610556Sadegh C, Macklis JDThe Journal of comparative neurologyEstablished monolayer differentiation of mouse embryonic stem cells generates heterogeneous neocortical-like neurons stalled at a stage equivalent to midcorticogenesis. J Comp Neurol. 2014 Aug 15; 522(12):2691-706.J Comp Neurol2014-04-12T00:00:002014Established monolayer differentiation of mouse embryonic stem cells generates heterogeneous neocortical-like neurons stalled at a stage equivalent to midcorticogenesis.24942076Sadegh C, Macklis JDThe Journal of comparative neurologyEstablished monolayer differentiation of mouse embryonic stem cells generates heterogeneous neocortical-like neurons stalled at a stage equivalent to midcorticogenesis. J Comp Neurol. 2014 Aug 15; 522(12):Spc1.J Comp Neurol2014-08-15T00:00:002014Established monolayer differentiation of mouse embryonic stem cells generates heterogeneous neocortical-like neurons stalled at a stage equivalent to midcorticogenesis.Authorship 3748782Authorship 3271782Authorship 4455551Authorship 3858853Authorship 417041Authorship 5051122Authorship 5277724Authorship 3661742Authorship 4180475Authorship 4355664Authorship 820655Authorship 5545882Authorship 927076Authorship 3033261Authorship 5161089Authorship 619344Authorship 4525922Authorship 1699532Authorship 3880065Authorship 5436712Authorship 5535603Authorship 5018307Authorship 4336554Authorship 5670275Authorship 634873Authorship 1878881Authorship 2324002Authorship 4634212Authorship 4817512Authorship 2646172Authorship 4006815Authorship 263102Authorship 328783Authorship 3697863Authorship 5476273Authorship 8059221425801576Jones AR, Troakes C, King A, Sahni V, De Jong S, Bossers K, Papouli E, Mirza M, Al-Sarraj S, Shaw CE, Shaw PJ, Kirby J, Veldink JH, Macklis JD, Powell JF, Al-Chalabi ANeurobiology of agingStratified gene expression analysis identifies major amyotrophic lateral sclerosis genes. Neurobiol Aging. 2015 May; 36(5):2006.e1-9.Neurobiol Aging2015-02-19T00:00:002015Stratified gene expression analysis identifies major amyotrophic lateral sclerosis genes.Authorship 3303422Authorship 2589774Authorship 338532Authorship 5430421Authorship 3031744Authorship 4774493Authorship 407772Authorship 841749625596590Jara JH, Genç B, Cox GA, Bohn MC, Roos RP, Macklis JD, Ulupinar E, Özdinler PHCerebral cortex (New York, N.Y. : 1991)Corticospinal Motor Neurons Are Susceptible to Increased ER Stress and Display Profound Degeneration in the Absence of UCHL1 Function. Cereb Cortex. 2015 Nov; 25(11):4259-72.Cereb Cortex2015-01-16T00:00:002015Corticospinal Motor Neurons Are Susceptible to Increased ER Stress and Display Profound Degeneration in the Absence of UCHL1 Function.Authorship 4157773Authorship 4742464Authorship 488702Authorship 4458457Authorship 4600915Authorship 4723526Authorship 866350626821816Kishi N, MacDonald JL, Ye J, Molyneaux BJ, Azim E, Macklis JDNature communicationsReduction of aberrant NF-?B signalling ameliorates Rett syndrome phenotypes in Mecp2-null mice. Nat Commun. 2016 Jan 29; 7:10520.Nat Commun2016-01-29T00:00:002016Reduction of aberrant NF-?B signalling ameliorates Rett syndrome phenotypes in Mecp2-null mice.Authorship 752752Authorship 3123812Authorship 3527733Authorship 875216927021939Sances S, Bruijn LI, Chandran S, Eggan K, Ho R, Klim JR, Livesey MR, Lowry E, Macklis JD, Rushton D, Sadegh C, Sareen D, Wichterle H, Zhang SC, Svendsen CNNature neuroscienceModeling ALS with motor neurons derived from human induced pluripotent stem cells. Nat Neurosci. 2016 Apr; 19(4):542-53.Nat Neurosci2016-04-01T00:00:002016Modeling ALS with motor neurons derived from human induced pluripotent stem cells.2005-08-26T00:00:002005-05-01T00:00:00Molecular Development of Corticospinal Motor Neurons in vivoSummerAuthorship 3956482Authorship 877968427100196Greig LC, Woodworth MB, Greppi C, Macklis JDNeuronCtip1 Controls Acquisition of Sensory Area Identity and Establishment of Sensory Input Fields in the Developing Neocortex. Neuron. 2016 04 20; 90(2):261-77.Neuron2016-04-20T00:00:002016Ctip1 Controls Acquisition of Sensory Area Identity and Establishment of Sensory Input Fields in the Developing Neocortex.2007-08-30T00:00:002007-05-30T00:00:00Computational Tools for Mapping Gene Regulatory Networks of Projection Neuron Subtype DifferentiationSummerAuthorship 880418627117402Woodworth MB, Greig LC, Liu KX, Ippolito GC, Tucker HO, Macklis JDCell reportsCtip1 Regulates the Balance between Specification of Distinct Projection Neuron Subtypes in Deep Cortical Layers. Cell Rep. 2016 05 03; 15(5):999-1012.Cell Rep2016-04-21T00:00:002016Ctip1 Regulates the Balance between Specification of Distinct Projection Neuron Subtypes in Deep Cortical Layers.Authorship 4716862001-08-31T00:00:002001-06-01T00:00:00Molecular Mechanisms of Cortical Projection Neuron Differentiation and NeurogenesisSummer2006-08-18T00:00:002006-06-19T00:00:00Genetic Expression Profile of Corticospinal Motor Neurons in Ctip2-deficient miceSummer27284167Shipman SL, Nivala J, Macklis JD, Church GMScience (New York, N.Y.)Molecular recordings by directed CRISPR spacer acquisition. Science. 2016 Jul 29; 353(6298):aaf1175.Science2016-06-09T00:00:002016Molecular recordings by directed CRISPR spacer acquisition.Authorship 886581527307230Fame RM, MacDonald JL, Dunwoodie SL, Takahashi E, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceCited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity. J Neurosci. 2016 06 15; 36(24):6403-19.J Neurosci2016-06-15T00:00:002016Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity.Authorship 912094Authorship 57379341Associate Professor6Fellow or Post Doc0Full Professor4Instructor5Other FacultyAuthorship 892015327321927Galazo MJ, Emsley JG, Macklis JDNeuronCorticothalamic Projection Neuron Development beyond Subtype Specification: Fog2 and Intersectional Controls Regulate Intraclass Neuronal Diversity. Neuron. 2016 07 06; 91(1):90-106.Neuron2016-06-16T00:00:002016Corticothalamic Projection Neuron Development beyond Subtype Specification: Fog2 and Intersectional Controls Regulate Intraclass Neuronal Diversity.Authorship 8948163Authorship 5697482GJF11GordFishellGord Fishell, Ph.D.152093Fishell, GordProfessor of Neurobiology2018-03-31MACKLIS, JEFFREY D2016-04-15Molecular development and diversity of callosal projection neuronsR56NS0933762012-08-31MACKLIS, JEFFREY D2000-09-21Neocortical Precursor Transplant for Circuitry RepairR37NS0415901996-07-31MACKLIS, JEFFREY D1991-08-01NEOCORTICAL NEURON GRAFTING INTO LASER-LESIONED MR MODELR29HD0284782021-05-31MACKLIS, JEFFREY D2002-08-01Molecular Controls over Neurogenesis, Subtype Development, and Diversity of Cortical Output Projection NeuronsR01NS0455232004-09-29MACKLIS, JEFFREY D2000-09-21NEOCORTICAL PRECURSOR TRANSPLANTS FOR CIRCUITRY REPAIRR01NS0415902018-01-31MACKLIS, JEFFREY D2012-05-01Molecular Mechanisms of CTIP2 Function in Corticospinal Motor Neuron DevelopmentR01NS0756722001-07-31MACKLIS, JEFFREY D1991-08-01NEOCORTICAL PRECURSOR TRANSPLANTS IN PHOTOLYTIC MR MODELR01HD0284782010-04-30MACKLIS, JEFFREY D2004-07-15Cortico-Spinal Repopulation from Precursors for RepairR01NS0495531991-06-30MACKLIS, JEFFREY D1988-07-26LASER KILLING OF FETAL NEURONSK08HD000859Principal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorPrincipal InvestigatorAuthorship 4137515Authorship 925538927707736Smith EC, Luc S, Croney DM, Woodworth MB, Greig LC, Fujiwara Y, Nguyen M, Sher F, Macklis JD, Bauer DE, Orkin SHBloodStrict in vivo specificity of the Bcl11a erythroid enhancer. Blood. 2016 11 10; 128(19):2338-2342.Blood2016-10-05T00:00:002016Strict in vivo specificity of the Bcl11a erythroid enhancer.Authorship 931700426874185Fame RM, Dehay C, Kennedy H, Macklis JDCerebral cortex (New York, N.Y. : 1991)Subtype-Specific Genes that Characterize Subpopulations of Callosal Projection Neurons in Mouse Identify Molecularly Homologous Populations in Macaque Cortex. Cereb Cortex. 2017 Mar 01; 27(3):1817-1830.Cereb Cortex2017-03-01T00:00:002017Subtype-Specific Genes that Characterize Subpopulations of Callosal Projection Neurons in Mouse Identify Molecularly Homologous Populations in Macaque Cortex.Authorship 949554Authorship 1923811Authorship 2372452Authorship 941650328010125Chen Z, Lin K, Macklis JD, Al-Chalabi AAmyotrophic lateral sclerosis & frontotemporal degenerationProposed association between the hexanucleotide repeat of C9orf72 and opposability index of the thumb. Amyotroph Lateral Scler Frontotemporal Degener. 2017 05; 18(3-4):175-181.Amyotroph Lateral Scler Frontotemporal Degener2016-12-23T00:00:002016Proposed association between the hexanucleotide repeat of C9orf72 and opposability index of the thumb.Authorship 9463361228053041Muralidharan B, Khatri Z, Maheshwari U, Gupta R, Roy B, Pradhan SJ, Karmodiya K, Padmanabhan H, Shetty AS, Balaji C, Kolthur-Seetharam U, Macklis JD, Galande S, Tole SThe Journal of neuroscience : the official journal of the Society for NeuroscienceLHX2 Interacts with the NuRD Complex and Regulates Cortical Neuron Subtype Determinants Fezf2 and Sox11. J Neurosci. 2017 01 04; 37(1):194-203.J Neurosci2017-01-04T00:00:002017LHX2 Interacts with the NuRD Complex and Regulates Cortical Neuron Subtype Determinants Fezf2 and Sox11.Authorship 9565631328178525Rodriguez-Muela N, Litterman NK, Norabuena EM, Mull JL, Galazo MJ, Sun C, Ng SY, Makhortova NR, White A, Lynes MM, Chung WK, Davidow LS, Macklis JD, Rubin LLCell reportsSingle-Cell Analysis of SMN Reveals Its Broader Role in Neuromuscular Disease. Cell Rep. 2017 02 07; 18(6):1484-1498.Cell Rep2017-02-07T00:00:002017Single-Cell Analysis of SMN Reveals Its Broader Role in Neuromuscular Disease.15111002Emsley JG, Arlotta P, Macklis JDTrends in neurosciencesStar-cross'd neurons: astroglial effects on neural repair in the adult mammalian CNS. Trends Neurosci. 2004 May; 27(5):238-40.Trends Neurosci2004-05-01T00:00:002004Star-cross'd neurons: astroglial effects on neural repair in the adult mammalian CNS.12019324Fricker-Gates RA, Shin JJ, Tai CC, Catapano LA, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceLate-stage immature neocortical neurons reconstruct interhemispheric connections and form synaptic contacts with increased efficiency in adult mouse cortex undergoing targeted neurodegeneration. J Neurosci. 2002 May 15; 22(10):4045-56.J Neurosci2002-05-15T00:00:002002Late-stage immature neocortical neurons reconstruct interhemispheric connections and form synaptic contacts with increased efficiency in adult mouse cortex undergoing targeted neurodegeneration.1520860Sheen VL, Dreyer EB, Macklis JDNeuroreportCalcium-mediated neuronal degeneration following singlet oxygen production. Neuroreport. 1992 Aug; 3(8):705-8.Neuroreport1992-08-01T00:00:001992Calcium-mediated neuronal degeneration following singlet oxygen production.10069337Lanier LM, Gates MA, Witke W, Menzies AS, Wehman AM, Macklis JD, Kwiatkowski D, Soriano P, Gertler FBNeuronMena is required for neurulation and commissure formation. Neuron. 1999 Feb; 22(2):313-25.Neuron1999-02-01T00:00:001999Mena is required for neurulation and commissure formation.11142025Gates MA, Fricker-Gates RA, Macklis JDProgress in brain researchReconstruction of cortical circuitry. Prog Brain Res. 2000; 127:115-56.Prog Brain Res2000-01-01T00:00:002000Reconstruction of cortical circuitry.17514196Molyneaux BJ, Arlotta P, Menezes JR, Macklis JDNature reviews. NeuroscienceNeuronal subtype specification in the cerebral cortex. Nat Rev Neurosci. 2007 Jun; 8(6):427-37.Nat Rev Neurosci2007-06-01T00:00:002007Neuronal subtype specification in the cerebral cortex.18160639Gao X, Arlotta P, Macklis JD, Chen JThe Journal of neuroscience : the official journal of the Society for NeuroscienceConditional knock-out of beta-catenin in postnatal-born dentate gyrus granule neurons results in dendritic malformation. J Neurosci. 2007 Dec 26; 27(52):14317-25.J Neurosci2007-12-26T00:00:002007Conditional knock-out of beta-catenin in postnatal-born dentate gyrus granule neurons results in dendritic malformation.18369770Catapano LA, Magavi SS, Macklis JDMethods in molecular biology (Clifton, N.J.)Neuroanatomical tracing of neuronal projections with Fluoro-Gold. Methods Mol Biol. 2008; 438:353-9.Methods Mol Biol2008-01-01T00:00:002008Neuroanatomical tracing of neuronal projections with Fluoro-Gold.11947937Magavi SS, Macklis JDBrain research. Developmental brain researchInduction of neuronal type-specific neurogenesis in the cerebral cortex of adult mice: manipulation of neural precursors in situ. Brain Res Dev Brain Res. 2002 Mar 31; 134(1-2):57-76.Brain Res Dev Brain Res2002-03-31T00:00:002002Induction of neuronal type-specific neurogenesis in the cerebral cortex of adult mice: manipulation of neural precursors in situ.10716243Catapano LA, Sheen VL, Leavitt BR, Macklis JDNeuroreportDifferentiation of transplanted neural precursors varies regionally in adults striatum. Neuroreport. 1999 Dec 16; 10(18):3971-7.Neuroreport1999-12-16T00:00:001999Differentiation of transplanted neural precursors varies regionally in adults striatum.15612019Mitchell BD, Macklis JDThe Journal of comparative neurologyLarge-scale maintenance of dual projections by callosal and frontal cortical projection neurons in adult mice. J Comp Neurol. 2005 Jan 31; 482(1):17-32.J Comp Neurol2005-01-31T00:00:002005Large-scale maintenance of dual projections by callosal and frontal cortical projection neurons in adult mice.10869838Gates MA, Tai CC, Macklis JDNeuroscienceNeocortical neurons lacking the protein-tyrosine kinase B receptor display abnormal differentiation and process elongation in vitro and in vivo. Neuroscience. 2000; 98(3):437-47.Neuroscience2000-01-01T00:00:002000Neocortical neurons lacking the protein-tyrosine kinase B receptor display abnormal differentiation and process elongation in vitro and in vivo.10448417Sheen VL, Arnold MW, Wang Y, Macklis JDExperimental neurologyNeural precursor differentiation following transplantation into neocortex is dependent on intrinsic developmental state and receptor competence. Exp Neurol. 1999 Jul; 158(1):47-62.Exp Neurol1999-07-01T00:00:001999Neural precursor differentiation following transplantation into neocortex is dependent on intrinsic developmental state and receptor competence.23141546Woodworth MB, Greig LC, Kriegstein AR, Macklis JDCellSnapShot: cortical development. Cell. 2012 Nov 09; 151(4):918-918.e1.Cell2012-11-09T00:00:002012SnapShot: cortical development.1836805Liu GT, Carrazana EJ, Macklis JD, Mikati MAJournal of clinical neuro-ophthalmologyDelayed oculogyric crises associated with striatocapsular infarction. J Clin Neuroophthalmol. 1991 Sep; 11(3):198-201.J Clin Neuroophthalmol1991-09-01T00:00:001991Delayed oculogyric crises associated with striatocapsular infarction.10222107Leavitt BR, Hernit-Grant CS, Macklis JDExperimental neurologyMature astrocytes transform into transitional radial glia within adult mouse neocortex that supports directed migration of transplanted immature neurons. Exp Neurol. 1999 May; 157(1):43-57.Exp Neurol1999-05-01T00:00:001999Mature astrocytes transform into transitional radial glia within adult mouse neocortex that supports directed migration of transplanted immature neurons.11007899Shin JJ, Fricker-Gates RA, Perez FA, Leavitt BR, Zurakowski D, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceTransplanted neuroblasts differentiate appropriately into projection neurons with correct neurotransmitter and receptor phenotype in neocortex undergoing targeted projection neuron degeneration. J Neurosci. 2000 Oct 01; 20(19):7404-16.J Neurosci2000-10-01T00:00:002000Transplanted neuroblasts differentiate appropriately into projection neurons with correct neurotransmitter and receptor phenotype in neocortex undergoing targeted projection neuron degeneration.Authorship 984380328535368Itoh Y, Poulopoulos A, Macklis JDDevelopmental cellUnfolding the Folding Problem of the Cerebral Cortex: Movin' and Groovin'. Dev Cell. 2017 05 22; 41(4):332-334.Dev Cell2017-05-22T00:00:002017Unfolding the Folding Problem of the Cerebral Cortex: Movin' and Groovin'.12904463Eyding D, Macklis JD, Neubacher U, Funke K, Wörgötter FThe Journal of neuroscience : the official journal of the Society for NeuroscienceSelective elimination of corticogeniculate feedback abolishes the electroencephalogram dependence of primary visual cortical receptive fields and reduces their spatial specificity. J Neurosci. 2003 Aug 06; 23(18):7021-33.J Neurosci2003-08-06T00:00:002003Selective elimination of corticogeniculate feedback abolishes the electroencephalogram dependence of primary visual cortical receptive fields and reduces their spatial specificity.2066774Macklis JD, Madison RDThe Journal of neuroscience : the official journal of the Society for NeuroscienceNeuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore. J Neurosci. 1991 Jul; 11(7):2055-62.J Neurosci1991-07-01T00:00:001991Neuroblastoma grafts are noninvasively removed within mouse neocortex by selective laser activation of intracellular photolytic chromophore.15717047Emsley JG, Mitchell BD, Magavi SS, Arlotta P, Macklis JDNeuroRx : the journal of the American Society for Experimental NeuroTherapeuticsThe repair of complex neuronal circuitry by transplanted and endogenous precursors. NeuroRx. 2004 Oct; 1(4):452-71.NeuroRx2004-10-01T00:00:002004The repair of complex neuronal circuitry by transplanted and endogenous precursors.18215621Lai T, Jabaudon D, Molyneaux BJ, Azim E, Arlotta P, Menezes JR, Macklis JDNeuronSOX5 controls the sequential generation of distinct corticofugal neuron subtypes. Neuron. 2008 Jan 24; 57(2):232-47.Neuron2008-01-24T00:00:002008SOX5 controls the sequential generation of distinct corticofugal neuron subtypes.Authorship 994035328700573Shipman SL, Nivala J, Macklis JD, Church GMNatureCRISPR-Cas encoding of a digital movie into the genomes of a population of living bacteria. Nature. 2017 07 20; 547(7663):345-349.Nature2017-07-12T00:00:002017CRISPR-Cas encoding of a digital movie into the genomes of a population of living bacteria.8366349Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceTransplanted neocortical neurons migrate selectively into regions of neuronal degeneration produced by chromophore-targeted laser photolysis. J Neurosci. 1993 Sep; 13(9):3848-63.J Neurosci1993-09-01T00:00:001993Transplanted neocortical neurons migrate selectively into regions of neuronal degeneration produced by chromophore-targeted laser photolysis.15519245Kishi N, Macklis JDMolecular and cellular neurosciencesMECP2 is progressively expressed in post-migratory neurons and is involved in neuronal maturation rather than cell fate decisions. Mol Cell Neurosci. 2004 Nov; 27(3):306-21.Mol Cell Neurosci2004-11-01T00:00:002004MECP2 is progressively expressed in post-migratory neurons and is involved in neuronal maturation rather than cell fate decisions.15711054Mitchell BD, Emsley JG, Magavi SS, Arlotta P, Macklis JDDevelopmental neuroscienceConstitutive and induced neurogenesis in the adult mammalian brain: manipulation of endogenous precursors toward CNS repair. Dev Neurosci. 2004 Mar-Aug; 26(2-4):101-17.Dev Neurosci2004-03-01T00:00:002004Constitutive and induced neurogenesis in the adult mammalian brain: manipulation of endogenous precursors toward CNS repair.7 Divinity AveFAS - Harvard, Bauer Laboratories, 1st floor,Depts of Stem Cell & Regenerative Biology; Neurology7 Divinity AveCambridge02138MA8635560Hernit-Grant CS, Macklis JDExperimental neurologyEmbryonic neurons transplanted to regions of targeted photolytic cell death in adult mouse somatosensory cortex re-form specific callosal projections. Exp Neurol. 1996 May; 139(1):131-42.Exp Neurol1996-05-01T00:00:001996Embryonic neurons transplanted to regions of targeted photolytic cell death in adult mouse somatosensory cortex re-form specific callosal projections.16492732Steele AD, Emsley JG, Ozdinler PH, Lindquist S, Macklis JDProceedings of the National Academy of Sciences of the United States of AmericaPrion protein (PrPc) positively regulates neural precursor proliferation during developmental and adult mammalian neurogenesis. Proc Natl Acad Sci U S A. 2006 Feb 28; 103(9):3416-21.Proc Natl Acad Sci U S A2006-02-21T00:00:002006Prion protein (PrPc) positively regulates neural precursor proliferation during developmental and adult mammalian neurogenesis.2224519Madison R, Macklis JD, Thies CBrain researchLatex nanosphere delivery system (LNDS): novel nanometer-sized carriers of fluorescent dyes and active agents selectively target neuronal subpopulations via uptake and retrograde transport. Brain Res. 1990 Jul 02; 522(1):90-8.Brain Res1990-07-02T00:00:001990Latex nanosphere delivery system (LNDS): novel nanometer-sized carriers of fluorescent dyes and active agents selectively target neuronal subpopulations via uptake and retrograde transport.17057708Ozdinler PH, Macklis JDNature neuroscienceIGF-I specifically enhances axon outgrowth of corticospinal motor neurons. Nat Neurosci. 2006 Nov; 9(11):1371-81.Nat Neurosci2006-10-22T00:00:002006IGF-I specifically enhances axon outgrowth of corticospinal motor neurons.19793993Molyneaux BJ, Arlotta P, Fame RM, MacDonald JL, MacQuarrie KL, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceNovel subtype-specific genes identify distinct subpopulations of callosal projection neurons. J Neurosci. 2009 Sep 30; 29(39):12343-54.J Neurosci2009-09-30T00:00:002009Novel subtype-specific genes identify distinct subpopulations of callosal projection neurons.2022-07-31MACKLIS, JEFFREY D2017-09-30Subcellular RNA-Proteome Mapping in Subtype- and Circuit-Specific Growth Cones: Development, Cell Biology, Disease, and RegenerationDP1NS106665Principal Investigator11268187Macklis JDNatureNeurobiology: New memories from new neurons. Nature. 2001 Mar 15; 410(6826):314-5, 317.Nature2001-03-15T00:00:002001Neurobiology: New memories from new neurons.1565255Macklis RM, Macklis JDNeurologyHistorical and phrenologic reflections on the nonmotor functions of the cerebellum: love under the tent? Neurology. 1992 Apr; 42(4):928-32.Neurology1992-04-01T00:00:001992Historical and phrenologic reflections on the nonmotor functions of the cerebellum: love under the tent?11951632Catapano LA, Magavi SS, Macklis JDMethods in molecular biology (Clifton, N.J.)Neuroanatomical tracing of neuronal projections with Fluoro-Gold. Methods Mol Biol. 2002; 198:299-304.Methods Mol Biol2002-01-01T00:00:002002Neuroanatomical tracing of neuronal projections with Fluoro-Gold.16291946Magavi SS, Mitchell BD, Szentirmai O, Carter BS, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceAdult-born and preexisting olfactory granule neurons undergo distinct experience-dependent modifications of their olfactory responses in vivo. J Neurosci. 2005 Nov 16; 25(46):10729-39.J Neurosci2005-11-16T00:00:002005Adult-born and preexisting olfactory granule neurons undergo distinct experience-dependent modifications of their olfactory responses in vivo.22632715Macklis JDNeuronHuman adult olfactory bulb neurogenesis? Novelty is the best policy. Neuron. 2012 May 24; 74(4):595-6.Neuron2012-05-24T00:00:002012Human adult olfactory bulb neurogenesis? Novelty is the best policy.15664173Arlotta P, Molyneaux BJ, Chen J, Inoue J, Kominami R, Macklis JDNeuronNeuronal subtype-specific genes that control corticospinal motor neuron development in vivo. Neuron. 2005 Jan 20; 45(2):207-21.Neuron2005-01-20T00:00:002005Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo.12626015Wörgötter F, Eyding D, Macklis JD, Funke KPhilosophical transactions of the Royal Society of London. Series B, Biological sciencesThe influence of the corticothalamic projection on responses in thalamus and cortex. Philos Trans R Soc Lond B Biol Sci. 2002 Dec 29; 357(1428):1823-34.Philos Trans R Soc Lond B Biol Sci2002-12-29T00:00:002002The influence of the corticothalamic projection on responses in thalamus and cortex.16939970Sohur US, Emsley JG, Mitchell BD, Macklis JDPhilosophical transactions of the Royal Society of London. Series B, Biological sciencesAdult neurogenesis and cellular brain repair with neural progenitors, precursors and stem cells. Philos Trans R Soc Lond B Biol Sci. 2006 Sep 29; 361(1473):1477-97.Philos Trans R Soc Lond B Biol Sci2006-09-29T00:00:002006Adult neurogenesis and cellular brain repair with neural progenitors, precursors and stem cells.17356684Emsley JG, Macklis JDNeuron glia biologyAstroglial heterogeneity closely reflects the neuronal-defined anatomy of the adult murine CNS. Neuron Glia Biol. 2006 Aug; 2(3):175-86.Neuron Glia Biol2006-08-01T00:00:002006Astroglial heterogeneity closely reflects the neuronal-defined anatomy of the adult murine CNS.2019-11-30MACKLIS, JEFFREY D2017-12-01Novel BEAM and R26-BEACON recombinase-based systems for mosaic analysis of gene functionR21NS104733Principal Investigator23575831Cederquist GY, Azim E, Shnider SJ, Padmanabhan H, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceLmo4 establishes rostral motor cortex projection neuron subtype diversity. J Neurosci. 2013 Apr 10; 33(15):6321-32.J Neurosci2013-04-10T00:00:002013Lmo4 establishes rostral motor cortex projection neuron subtype diversity.2308380Macklis JD, Madison RDJournal of neuroscience methodsProgressive incorporation of propidium iodide in cultured mouse neurons correlates with declining electrophysiological status: a fluorescence scale of membrane integrity. J Neurosci Methods. 1990 Jan; 31(1):43-6.J Neurosci Methods1990-01-01T00:00:001990Progressive incorporation of propidium iodide in cultured mouse neurons correlates with declining electrophysiological status: a fluorescence scale of membrane integrity.Authorship 10316961129222517Frangeul L, Kehayas V, Sanchez-Mut JV, Fièvre S, Krishna-K K, Pouchelon G, Telley L, Bellone C, Holtmaat A, Gräff J, Macklis JD, Jabaudon DNature communicationsInput-dependent regulation of excitability controls dendritic maturation in somatosensory thalamocortical neurons. Nat Commun. 2017 12 08; 8(1):2015.Nat Commun2017-12-08T00:00:002017Input-dependent regulation of excitability controls dendritic maturation in somatosensory thalamocortical neurons.20025874Kishi N, Macklis JDExperimental neurologyMeCP2 functions largely cell-autonomously, but also non-cell-autonomously, in neuronal maturation and dendritic arborization of cortical pyramidal neurons. Exp Neurol. 2010 Mar; 222(1):51-8.Exp Neurol2009-12-16T00:00:002009MeCP2 functions largely cell-autonomously, but also non-cell-autonomously, in neuronal maturation and dendritic arborization of cortical pyramidal neurons.2743289Schick RM, Jolesz F, Barnes PD, Macklis JDComputerized medical imaging and graphics : the official journal of the Computerized Medical Imaging SocietyMR diagnosis of dural venous sinus thrombosis complicating L-asparaginase therapy. Comput Med Imaging Graph. 1989 Jul-Aug; 13(4):319-27.Comput Med Imaging Graph1989-07-01T00:00:001989MR diagnosis of dural venous sinus thrombosis complicating L-asparaginase therapy.18369769Magavi SS, Macklis JDMethods in molecular biology (Clifton, N.J.)Immunocytochemical analysis of neuronal differentiation. Methods Mol Biol. 2008; 438:345-52.Methods Mol Biol2008-01-01T00:00:002008Immunocytochemical analysis of neuronal differentiation.19366868Azim E, Shnider SJ, Cederquist GY, Sohur US, Macklis JDCerebral cortex (New York, N.Y. : 1991)Lmo4 and Clim1 progressively delineate cortical projection neuron subtypes during development. Cereb Cortex. 2009 Jul; 19 Suppl 1:i62-9.Cereb Cortex2009-04-14T00:00:002009Lmo4 and Clim1 progressively delineate cortical projection neuron subtypes during development.3841019Macklis JD, Madison RBrain researchUnfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis. Brain Res. 1985 Dec 16; 359(1-2):158-65.Brain Res1985-12-16T00:00:001985Unfocused laser illumination kills dye-targeted mouse neurons by selective photothermolysis.7821398Sheen VL, Macklis JDExperimental neurologyApoptotic mechanisms in targeted neuronal cell death by chromophore-activated photolysis. Exp Neurol. 1994 Nov; 130(1):67-81.Exp Neurol1994-11-01T00:00:001994Apoptotic mechanisms in targeted neuronal cell death by chromophore-activated photolysis.8613770Sheen VL, Macklis JDThe Journal of neuroscience : the official journal of the Society for NeuroscienceTargeted neocortical cell death in adult mice guides migration and differentiation of transplanted embryonic neurons. J Neurosci. 1995 Dec; 15(12):8378-92.J Neurosci1995-12-01T00:00:001995Targeted neocortical cell death in adult mice guides migration and differentiation of transplanted embryonic neurons.8914798Snyder EY, Macklis JDClinical neuroscience (New York, N.Y.)Multipotent neural progenitor or stem-like cells may be uniquely suited for therapy for some neurodegenerative conditions. Clin Neurosci. 1995-1996; 3(5):310-6.Clin Neurosci1995-01-01T00:00:001995Multipotent neural progenitor or stem-like cells may be uniquely suited for therapy for some neurodegenerative conditions.Authorship 1043149429379878MacDonald JL, Fame RM, Gillis-Buck EM, Macklis JDeNeuroCaveolin1 Identifies a Specific Subpopulation of Cerebral Cortex Callosal Projection Neurons (CPN) Including Dual Projecting Cortical Callosal/Frontal Projection Neurons (CPN/FPN). eNeuro. 2018 Jan-Feb; 5(1).eNeuro2018-01-18T00:00:002018Caveolin1 Identifies a Specific Subpopulation of Cerebral Cortex Callosal Projection Neurons (CPN) Including Dual Projecting Cortical Callosal/Frontal Projection Neurons (CPN/FPN).12543275Arlotta P, Magavi SS, Macklis JDExperimental gerontologyMolecular manipulation of neural precursors in situ: induction of adult cortical neurogenesis. Exp Gerontol. 2003 Jan-Feb; 38(1-2):173-82.Exp Gerontol2003-01-01T00:00:002003Molecular manipulation of neural precursors in situ: induction of adult cortical neurogenesis.15913880Emsley JG, Mitchell BD, Kempermann G, Macklis JDProgress in neurobiologyAdult neurogenesis and repair of the adult CNS with neural progenitors, precursors, and stem cells. Prog Neurobiol. 2005 Apr; 75(5):321-41.Prog Neurobiol2005-04-01T00:00:002005Adult neurogenesis and repair of the adult CNS with neural progenitors, precursors, and stem cells.16009125Arlotta P, Macklis JDCellArcheo-cell biology: carbon dating is not just for pots and dinosaurs. Cell. 2005 Jul 15; 122(1):4-6.Cell2005-07-15T00:00:002005Archeo-cell biology: carbon dating is not just for pots and dinosaurs.16157277Molyneaux BJ, Arlotta P, Hirata T, Hibi M, Macklis JDNeuronFezl is required for the birth and specification of corticospinal motor neurons. Neuron. 2005 Sep 15; 47(6):817-31.Neuron2005-09-15T00:00:002005Fezl is required for the birth and specification of corticospinal motor neurons.16225831Kishi N, Macklis JDJournal of child neurologyDissecting MECP2 function in the central nervous system. J Child Neurol. 2005 Sep; 20(9):753-9.J Child Neurol2005-09-01T00:00:002005Dissecting MECP2 function in the central nervous system.