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Keywords
Last Name
Institution

John M Asara, PH.D.

TitleAssistant Professor of Medicine
InstitutionBeth Israel Deaconess Medical Center
DepartmentMedicine
AddressCenter for Life Science
BIDMC - Rm 409
3 Blackfan Circle
Boston MA 02115
Phone617/735-2651
Fax617/735-2655

 Biography 
 awards and honors
2007 - 10 out of 100 Top Scientific Discoveries
2008 - Plenary Lecturer
2009 - Young Investigator Award
2010 - Faculty of 1000, Biology, Signal Transduction
2010 - Outstanding Poster Award
2012 - Shared Instrumentation Grant Award

 Overview 
 overview
Research activities in the Asara lab focusses on developing -omics (proteomics and metabolomics) approaches to personalized medicine by analyzing cancer cells and tumor samples in order help make appropriate therapeutic decisions with "smart" drugs. This is accomplished by understanding how cancer cells are signaling for growth and proliferation and how signaling talks to metabolic processes in order to develop mass spec quantitative assays. We are developing hypothesis directed proteomics and metabolomics strategies to understand the fundamental drivers of disease in cancers such as multiple myeloma, lung cancer and prostate cancer by performing protein-protein interaction studies, immunoprecipitation (IP)-tandem mass spectrometry (MS/MS), quantitative phosphoproteomics and metabolic flux analyses. Taken together, this information is vital in selecting the appropriate use of newly developed drugs. In addition to our internal research, we are involved in many collaborative projects that are coordinated through our state-of-the-art mass spectrometry technologies and services through our mass spectrometry facility. We were just awarded a NIH Shared Instrumentation Grant for a Thermo Velos Elite Orbitrap mass spectrometer to enhance the sensitivity and throughput of our personalized medicine approaches.


 Research 
 research resources
This researcher has shared information about their research resources
in the eagle-i Network. To update or add resource records, contact
eagle-i@hms.harvard.edu.
Mass Spectrometry Core (BIDMC) - Core services (5), Instruments (9) and Protocols (8)
DF/HCC Cancer Proteomics Center - Core services (5) and Instruments (11)

 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Ding J, Li T, Wang X, Zhao E, Choi JH, Yang L, Zha Y, Dong Z, Huang S, Asara JM, Cui H, Ding HF. The Histone H3 Methyltransferase G9A Epigenetically Activates the Serine-Glycine Synthesis Pathway to Sustain Cancer Cell Survival and Proliferation. Cell Metab. 2013 Dec 3; 18(6):896-907.
    View in: PubMed
  2. Parkhitko AA, Priolo C, Coloff JL, Yun J, Wu JJ, Mizumura K, Xu W, Malinowska IA, Yu J, Kwiatkowski DJ, Locasale JW, Asara JM, Choi AM, Finkel T, Henske EP. Autophagy-Dependent Metabolic Reprogramming Sensitizes TSC2-Deficient Cells to the Antimetabolite 6-Aminonicotinamide. Mol Cancer Res. 2014 Jan; 12(1):48-57.
    View in: PubMed
  3. Emerling BM, Hurov JB, Poulogiannis G, Tsukazawa KS, Choo-Wing R, Wulf GM, Bell EL, Shim HS, Lamia KA, Rameh LE, Bellinger G, Sasaki AT, Asara JM, Yuan X, Bullock A, Denicola GM, Song J, Brown V, Signoretti S, Cantley LC. Depletion of a Putatively Druggable Class of Phosphatidylinositol Kinases Inhibits Growth of p53-Null Tumors. Cell. 2013 Nov 7; 155(4):844-57.
    View in: PubMed
  4. Shyh-Chang N, Zhu H, Yvanka de Soysa T, Shinoda G, Seligson MT, Tsanov KM, Nguyen L, Asara JM, Cantley LC, Daley GQ. Lin28 enhances tissue repair by reprogramming cellular metabolism. Cell. 2013 Nov 7; 155(4):778-92.
    View in: PubMed
  5. Liu P, Gan W, Inuzuka H, Lazorchak AS, Gao D, Arojo O, Liu D, Wan L, Zhai B, Yu Y, Yuan M, Kim BM, Shaik S, Menon S, Gygi SP, Lee TH, Asara JM, Manning BD, Blenis J, Su B, Wei W. Sin1 phosphorylation impairs mTORC2 complex integrity and inhibits downstream Akt signalling to suppress tumorigenesis. Nat Cell Biol. 2013 Nov; 15(11):1340-50.
    View in: PubMed
  6. Shen CH, Yuan P, Perez-Lorenzo R, Zhang Y, Lee SX, Ou Y, Asara JM, Cantley LC, Zheng B. Phosphorylation of BRAF by AMPK Impairs BRAF-KSR1 Association and Cell Proliferation. Mol Cell. 2013 Oct 24; 52(2):161-72.
    View in: PubMed
  7. Reschke M, Clohessy JG, Seitzer N, Goldstein DP, Breitkopf SB, Schmolze DB, Ala U, Asara JM, Beck AH, Pandolfi PP. Characterization and analysis of the composition and dynamics of the Mammalian riboproteome. Cell Rep. 2013 Sep 26; 4(6):1276-87.
    View in: PubMed
  8. Yeo H, Lyssiotis CA, Zhang Y, Ying H, Asara JM, Cantley LC, Paik JH. FoxO3 coordinates metabolic pathways to maintain redox balance in neural stem cells. EMBO J. 2013 Oct 2; 32(19):2589-602.
    View in: PubMed
  9. Shinoda G, Shyh-Chang N, Soysa TY, Zhu H, Seligson MT, Shah SP, Abo-Sido N, Yabuuchi A, Hagan JP, Gregory RI, Asara JM, Cantley LC, Moss EG, Daley GQ. Fetal deficiency of lin28 programs life-long aberrations in growth and glucose metabolism. Stem Cells. 2013 Aug; 31(8):1563-73.
    View in: PubMed
  10. Liu Y, Marks K, Cowley GS, Carretero J, Liu Q, Nieland TJ, Xu C, Cohoon TJ, Gao P, Zhang Y, Chen Z, Altabef AB, Tchaicha JH, Wang X, Choe S, Driggers EM, Zhang J, Bailey ST, Sharpless NE, Hayes DN, Patel NM, Janne PA, Bardeesy N, Engelman JA, Manning BD, Shaw RJ, Asara JM, Scully R, Kimmelman A, Byers LA, Gibbons DL, Wistuba II, Heymach JV, Kwiatkowski DJ, Kim WY, Kung AL, Gray NS, Root DE, Cantley LC, Wong KK. Metabolic and functional genomic studies identify deoxythymidylate kinase as a target in LKB1-mutant lung cancer. Cancer Discov. 2013 Aug; 3(8):870-9.
    View in: PubMed
  11. Chen S, Jiang X, Gewinner CA, Asara JM, Simon NI, Cai C, Cantley LC, Balk SP. Tyrosine Kinase BMX Phosphorylates Phosphotyrosine-Primed Motif Mediating the Activation of Multiple Receptor Tyrosine Kinases. Sci Signal. 2013; 6(277):ra40.
    View in: PubMed
  12. Wang WJ, Tay HG, Soni R, Perumal GS, Goll MG, Macaluso FP, Asara JM, Amack JD, Bryan Tsou MF. CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. Nat Cell Biol. 2013 Jun; 15(6):591-601.
    View in: PubMed
  13. Riedel CG, Dowen RH, Lourenco GF, Kirienko NV, Heimbucher T, West JA, Bowman SK, Kingston RE, Dillin A, Asara JM, Ruvkun G. DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. Nat Cell Biol. 2013 May; 15(5):491-501.
    View in: PubMed
  14. Parent KN, Takagi Y, Cardone G, Olson NH, Ericsson M, Yang M, Lee Y, Asara JM, Fichorova RN, Baker TS, Nibert ML. Structure of a Protozoan Virus from the Human Genitourinary Parasite Trichomonas vaginalis. MBio. 2013; 4(2).
    View in: PubMed
  15. Son J, Lyssiotis CA, Ying H, Wang X, Hua S, Ligorio M, Perera RM, Ferrone CR, Mullarky E, Shyh-Chang N, Kang Y, Fleming JB, Bardeesy N, Asara JM, Haigis MC, DePinho RA, Cantley LC, Kimmelman AC. Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway. Nature. 2013 Apr 4; 496(7443):101-5.
    View in: PubMed
  16. Wu N, Zheng B, Shaywitz A, Dagon Y, Tower C, Bellinger G, Shen CH, Wen J, Asara J, McGraw TE, Kahn BB, Cantley LC. AMPK-Dependent Degradation of TXNIP upon Energy Stress Leads to Enhanced Glucose Uptake via GLUT1. Mol Cell. 2013 Mar 28; 49(6):1167-75.
    View in: PubMed
  17. Ben-Sahra I, Howell JJ, Asara JM, Manning BD. Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1. Science. 2013 Mar 15; 339(6125):1323-8.
    View in: PubMed
  18. Nicolay BN, Gameiro PA, Tschöp K, Korenjak M, Heilmann AM, Asara JM, Stephanopoulos G, Iliopoulos O, Dyson NJ. Loss of RBF1 changes glutamine catabolism. Genes Dev. 2013 Jan 15; 27(2):182-96.
    View in: PubMed
  19. Tanos BE, Yang HJ, Soni R, Wang WJ, Macaluso FP, Asara JM, Tsou MF. Centriole distal appendages promote membrane docking, leading to cilia initiation. Genes Dev. 2013 Jan 15; 27(2):163-8.
    View in: PubMed
  20. Anastasiou D, Yu Y, Israelsen WJ, Jiang JK, Boxer MB, Hong BS, Tempel W, Dimov S, Shen M, Jha A, Yang H, Mattaini KR, Metallo CM, Fiske BP, Courtney KD, Malstrom S, Khan TM, Kung C, Skoumbourdis AP, Veith H, Southall N, Walsh MJ, Brimacombe KR, Leister W, Lunt SY, Johnson ZR, Yen KE, Kunii K, Davidson SM, Christofk HR, Austin CP, Inglese J, Harris MH, Asara JM, Stephanopoulos G, Salituro FG, Jin S, Dang L, Auld DS, Park HW, Cantley LC, Thomas CJ, Vander Heiden MG. Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis. Nat Chem Biol. 2012 Dec; 8(12):1008.
    View in: PubMed
  21. Webhofer C, Gormanns P, Reckow S, Lebar M, Maccarrone G, Ludwig T, Pütz B, Asara JM, Holsboer F, Sillaber I, Zieglgänsberger W, Turck CW. Proteomic and metabolomic profiling reveals time-dependent changes in hippocampal metabolism upon paroxetine treatment and biomarker candidates. J Psychiatr Res. 2013 Mar; 47(3):289-98.
    View in: PubMed
  22. Shyh-Chang N, Locasale JW, Lyssiotis CA, Zheng Y, Teo RY, Ratanasirintrawoot S, Zhang J, Onder T, Unternaehrer JJ, Zhu H, Asara JM, Daley GQ, Cantley LC. Influence of threonine metabolism on s-adenosylmethionine and histone methylation. Science. 2013 Jan 11; 339(6116):222-6.
    View in: PubMed
  23. Anastasiou D, Yu Y, Israelsen WJ, Jiang JK, Boxer MB, Hong BS, Tempel W, Dimov S, Shen M, Jha A, Yang H, Mattaini KR, Metallo CM, Fiske BP, Courtney KD, Malstrom S, Khan TM, Kung C, Skoumbourdis AP, Veith H, Southall N, Walsh MJ, Brimacombe KR, Leister W, Lunt SY, Johnson ZR, Yen KE, Kunii K, Davidson SM, Christofk HR, Austin CP, Inglese J, Harris MH, Asara JM, Stephanopoulos G, Salituro FG, Jin S, Dang L, Auld DS, Park HW, Cantley LC, Thomas CJ, Vander Heiden MG. Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis. Nat Chem Biol. 2012 Oct; 8(10):839-47.
    View in: PubMed
  24. Zheng Y, Gierut J, Wang Z, Miao J, Asara JM, Tyner AL. Protein tyrosine kinase 6 protects cells from anoikis by directly phosphorylating focal adhesion kinase and activating AKT. Oncogene. 2013 Sep 5; 32(36):4304-12.
    View in: PubMed
  25. Breitkopf SB, Yuan M, Pihan GA, Asara JM. Detection of a rare BCR-ABL tyrosine kinase fusion protein in H929 multiple myeloma cells using immunoprecipitation (IP)-tandem mass spectrometry (MS/MS). Proc Natl Acad Sci U S A. 2012 Oct 2; 109(40):16190-5.
    View in: PubMed
  26. Juvekar A, Burga LN, Hu H, Lunsford EP, Ibrahim YH, Balmañà J, Rajendran A, Papa A, Spencer K, Lyssiotis CA, Nardella C, Pandolfi PP, Baselga J, Scully R, Asara JM, Cantley LC, Wulf GM. Combining a PI3K Inhibitor with a PARP Inhibitor Provides an Effective Therapy for BRCA1-Related Breast Cancer. Cancer Discov. 2012 Nov; 2(11):1048-63.
    View in: PubMed
  27. Mack HI, Zheng B, Asara J, Thomas SM. AMPK-dependent phosphorylation of ULK1 regulates ATG9 localization. Autophagy. 2012 Aug; 8(8):1197-214.
    View in: PubMed
  28. Dibble CC, Elis W, Menon S, Qin W, Klekota J, Asara JM, Finan PM, Kwiatkowski DJ, Murphy LO, Manning BD. TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1. Mol Cell. 2012 Aug 24; 47(4):535-46.
    View in: PubMed
  29. Li M, Aliotta JM, Asara JM, Tucker L, Quesenberry P, Lally M, Ramratnam B. Quantitative proteomic analysis of exosomes from HIV-1-infected lymphocytic cells. Proteomics. 2012 Jul; 12(13):2203-11.
    View in: PubMed
  30. Fukushima H, Matsumoto A, Inuzuka H, Zhai B, Lau AW, Wan L, Gao D, Shaik S, Yuan M, Gygi SP, Jimi E, Asara JM, Nakayama K, Nakayama KI, Wei W. SCF(Fbw7) modulates the NFkB signaling pathway by targeting NFkB2 for ubiquitination and destruction. Cell Rep. 2012 May 31; 1(5):434-43.
    View in: PubMed
  31. Brown JR, Hanna M, Tesar B, Werner L, Pochet N, Asara JM, Wang YE, Dal Cin P, Fernandes SM, Thompson C, Macconaill L, Wu CJ, Van de Peer Y, Correll M, Regev A, Neuberg D, Freedman AS. Integrative genomic analysis implicates gain of PIK3CA at 3q26 and MYC at 8q24 in chronic lymphocytic leukemia. Clin Cancer Res. 2012 Jul 15; 18(14):3791-802.
    View in: PubMed
  32. Turke AB, Song Y, Costa C, Cook R, Arteaga CL, Asara JM, Engelman JA. MEK inhibition leads to PI3K/AKT activation by relieving a negative feedback on ERBB receptors. Cancer Res. 2012 Jul 1; 72(13):3228-37.
    View in: PubMed
  33. Ying H, Kimmelman AC, Lyssiotis CA, Hua S, Chu GC, Fletcher-Sananikone E, Locasale JW, Son J, Zhang H, Coloff JL, Yan H, Wang W, Chen S, Viale A, Zheng H, Paik JH, Lim C, Guimaraes AR, Martin ES, Chang J, Hezel AF, Perry SR, Hu J, Gan B, Xiao Y, Asara JM, Weissleder R, Wang YA, Chin L, Cantley LC, DePinho RA. Oncogenic Kras maintains pancreatic tumors through regulation of anabolic glucose metabolism. Cell. 2012 Apr 27; 149(3):656-70.
    View in: PubMed
  34. Yuan M, Breitkopf SB, Yang X, Asara JM. A positive/negative ion-switching, targeted mass spectrometry-based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue. Nat Protoc. 2012 May; 7(5):872-81.
    View in: PubMed
  35. Breitkopf SB, Asara JM. Determining in vivo phosphorylation sites using mass spectrometry. Curr Protoc Mol Biol. 2012 Apr; Chapter 18:Unit18.19.1-27.
    View in: PubMed
  36. Kategaya LS, Hilliard A, Zhang L, Asara JM, Ptácek LJ, Fu YH. Casein kinase 1 proteomics reveal prohibitin 2 function in molecular clock. PLoS One. 2012; 7(2):e31987.
    View in: PubMed
  37. Locasale JW, Melman T, Song S, Yang X, Swanson KD, Cantley LC, Wong ET, Asara JM. Metabolomics of human cerebrospinal fluid identifies signatures of malignant glioma. Mol Cell Proteomics. 2012 Jun; 11(6):M111.014688.
    View in: PubMed
  38. Lu B, Asara JM, Sanda MG, Arredouani MS. The role of the transcription factor SIM2 in prostate cancer. PLoS One. 2011; 6(12):e28837.
    View in: PubMed
  39. Libert S, Pointer K, Bell EL, Das A, Cohen DE, Asara JM, Kapur K, Bergmann S, Preisig M, Otowa T, Kendler KS, Chen X, Hettema JM, van den Oord EJ, Rubio JP, Guarente L. SIRT1 activates MAO-A in the brain to mediate anxiety and exploratory drive. Cell. 2011 Dec 23; 147(7):1459-72.
    View in: PubMed
  40. Zheng Y, Asara JM, Tyner AL. Protein-tyrosine kinase 6 promotes peripheral adhesion complex formation and cell migration by phosphorylating p130 CRK-associated substrate. J Biol Chem. 2012 Jan 2; 287(1):148-58.
    View in: PubMed
  41. Anastasiou D, Poulogiannis G, Asara JM, Boxer MB, Jiang JK, Shen M, Bellinger G, Sasaki AT, Locasale JW, Auld DS, Thomas CJ, Vander Heiden MG, Cantley LC. Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. Science. 2011 Dec 2; 334(6060):1278-83.
    View in: PubMed
  42. Friedman AA, Tucker G, Singh R, Yan D, Vinayagam A, Hu Y, Binari R, Hong P, Sun X, Porto M, Pacifico S, Murali T, Finley RL, Asara JM, Berger B, Perrimon N. Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Sci Signal. 2011; 4(196):rs10.
    View in: PubMed
  43. Gao D, Inuzuka H, Tan MK, Fukushima H, Locasale JW, Liu P, Wan L, Zhai B, Chin YR, Shaik S, Lyssiotis CA, Gygi SP, Toker A, Cantley LC, Asara JM, Harper JW, Wei W. mTOR drives its own activation via SCF(ßTrCP)-dependent degradation of the mTOR inhibitor DEPTOR. Mol Cell. 2011 Oct 21; 44(2):290-303.
    View in: PubMed
  44. Kelly AD, Breitkopf SB, Yuan M, Goldsmith J, Spentzos D, Asara JM. Metabolomic profiling from formalin-fixed, paraffin-embedded tumor tissue using targeted LC/MS/MS: application in sarcoma. PLoS One. 2011; 6(10):e25357.
    View in: PubMed
  45. Yi CH, Pan H, Seebacher J, Jang IH, Hyberts SG, Heffron GJ, Vander Heiden MG, Yang R, Li F, Locasale JW, Sharfi H, Zhai B, Rodriguez-Mias R, Luithardt H, Cantley LC, Daley GQ, Asara JM, Gygi SP, Wagner G, Liu CF, Yuan J. Metabolic regulation of protein N-alpha-acetylation by Bcl-xL promotes cell survival. Cell. 2011 Aug 19; 146(4):607-20.
    View in: PubMed
  46. Yuan S, Yu X, Asara JM, Heuser JE, Ludtke SJ, Akey CW. The holo-apoptosome: activation of procaspase-9 and interactions with caspase-3. Structure. 2011 Aug 10; 19(8):1084-96.
    View in: PubMed
  47. Lee JY, Chiu YH, Asara J, Cantley LC. Inhibition of PI3K binding to activators by serine phosphorylation of PI3K regulatory subunit p85alpha Src homology-2 domains. Proc Natl Acad Sci U S A. 2011 Aug 23; 108(34):14157-62.
    View in: PubMed
  48. Christofk HR, Wu N, Cantley LC, Asara JM. Proteomic screening method for phosphopeptide motif binding proteins using peptide libraries. J Proteome Res. 2011 Sep 2; 10(9):4158-64.
    View in: PubMed
  49. Locasale JW, Grassian AR, Melman T, Lyssiotis CA, Mattaini KR, Bass AJ, Heffron G, Metallo CM, Muranen T, Sharfi H, Sasaki AT, Anastasiou D, Mullarky E, Vokes NI, Sasaki M, Beroukhim R, Stephanopoulos G, Ligon AH, Meyerson M, Richardson AL, Chin L, Wagner G, Asara JM, Brugge JS, Cantley LC, Vander Heiden MG. Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis. Nat Genet. 2011 Sep; 43(9):869-74.
    View in: PubMed
  50. Yang X, Turke AB, Qi J, Song Y, Rexer BN, Miller TW, Jänne PA, Arteaga CL, Cantley LC, Engelman JA, Asara JM. Using tandem mass spectrometry in targeted mode to identify activators of class IA PI3K in cancer. Cancer Res. 2011 Sep 15; 71(18):5965-75.
    View in: PubMed
  51. Tun-Kyi A, Finn G, Greenwood A, Nowak M, Lee TH, Asara JM, Tsokos GC, Fitzgerald K, Israel E, Li X, Exley M, Nicholson LK, Lu KP. Essential role for the prolyl isomerase Pin1 in Toll-like receptor signaling and type I interferon-mediated immunity. Nat Immunol. 2011 Aug; 12(8):733-41.
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  52. Sasaki AT, Carracedo A, Locasale JW, Anastasiou D, Takeuchi K, Kahoud ER, Haviv S, Asara JM, Pandolfi PP, Cantley LC. Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors. Sci Signal. 2011; 4(163):ra13.
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  53. Aboulaich N, Chui PC, Asara JM, Flier JS, Maratos-Flier E. Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism. Diabetes. 2011 Mar; 60(3):757-65.
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  54. Simabuco FM, Asara JM, Guerrero MC, Libermann TA, Zerbini LF, Ventura AM. Structural analysis of human respiratory syncytial virus p protein: identification of intrinsically disordered domains. Braz J Microbiol. 2011 Jan; 42(1):340-5.
    View in: PubMed
  55. Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase connects energy sensing to mitophagy. Science. 2011 Jan 28; 331(6016):456-61.
    View in: PubMed
  56. Tigno-Aranjuez JT, Asara JM, Abbott DW. Inhibition of RIP2's tyrosine kinase activity limits NOD2-driven cytokine responses. Genes Dev. 2010 Dec 1; 24(23):2666-77.
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  57. Vander Heiden MG, Locasale JW, Swanson KD, Sharfi H, Heffron GJ, Amador-Noguez D, Christofk HR, Wagner G, Rabinowitz JD, Asara JM, Cantley LC. Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science. 2010 Sep 17; 329(5998):1492-9.
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  58. Soltoff SP, Asara JM, Hedden L. Regulation and identification of Na,K-ATPase alpha1 subunit phosphorylation in rat parotid acinar cells. J Biol Chem. 2010 Nov 19; 285(47):36330-8.
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  59. Inuzuka H, Tseng A, Gao D, Zhai B, Zhang Q, Shaik S, Wan L, Ang XL, Mock C, Yin H, Stommel JM, Gygi S, Lahav G, Asara J, Xiao ZX, Kaelin WG, Harper JW, Wei W. Phosphorylation by casein kinase I promotes the turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin ligase. Cancer Cell. 2010 Aug 9; 18(2):147-59.
    View in: PubMed
  60. Zheng Y, Peng M, Wang Z, Asara JM, Tyner AL. Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor. Mol Cell Biol. 2010 Sep; 30(17):4280-92.
    View in: PubMed
  61. Nhek S, Ngo M, Yang X, Ng MM, Field SJ, Asara JM, Ridgway ND, Toker A. Regulation of oxysterol-binding protein Golgi localization through protein kinase D-mediated phosphorylation. Mol Biol Cell. 2010 Jul 1; 21(13):2327-37.
    View in: PubMed
  62. Jiang X, Chen S, Asara JM, Balk SP. Phosphoinositide 3-kinase pathway activation in phosphate and tensin homolog (PTEN)-deficient prostate cancer cells is independent of receptor tyrosine kinases and mediated by the p110beta and p110delta catalytic subunits. J Biol Chem. 2010 May 14; 285(20):14980-9.
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  63. Gwinn DM, Asara JM, Shaw RJ. Raptor is phosphorylated by cdc2 during mitosis. PLoS One. 2010; 5(2):e9197.
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  64. Palka-Hamblin HL, Gierut JJ, Bie W, Brauer PM, Zheng Y, Asara JM, Tyner AL. Identification of beta-catenin as a target of the intracellular tyrosine kinase PTK6. J Cell Sci. 2010 Jan 15; 123(Pt 2):236-45.
    View in: PubMed
  65. Li M, Aliotta JM, Asara JM, Wu Q, Dooner MS, Tucker LD, Wells A, Quesenberry PJ, Ramratnam B. Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture. J Biol Chem. 2010 Feb 26; 285(9):6285-97.
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  66. Kesavan K, Ratliff J, Johnson EW, Dahlberg W, Asara JM, Misra P, Frangioni JV, Jacoby DB. Annexin A2 is a molecular target for TM601, a peptide with tumor-targeting and anti-angiogenic effects. J Biol Chem. 2010 Feb 12; 285(7):4366-74.
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  67. Yang X, Friedman A, Nagpal S, Perrimon N, Asara JM. Use of a label-free quantitative platform based on MS/MS average TIC to calculate dynamics of protein complexes in insulin signaling. J Biomol Tech. 2009 Dec; 20(5):272-7.
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  68. Chen YJ, Dominguez-Brauer C, Wang Z, Asara JM, Costa RH, Tyner AL, Lau LF, Raychaudhuri P. A conserved phosphorylation site within the forkhead domain of FoxM1B is required for its activation by cyclin-CDK1. J Biol Chem. 2009 Oct 30; 284(44):30695-707.
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  69. Dibble CC, Asara JM, Manning BD. Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1. Mol Cell Biol. 2009 Nov; 29(21):5657-70.
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  70. Hutti JE, Shen RR, Abbott DW, Zhou AY, Sprott KM, Asara JM, Hahn WC, Cantley LC. Phosphorylation of the tumor suppressor CYLD by the breast cancer oncogene IKKepsilon promotes cell transformation. Mol Cell. 2009 May 14; 34(4):461-72.
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  71. Schweitzer MH, Zheng W, Organ CL, Avci R, Suo Z, Freimark LM, Lebleu VS, Duncan MB, Vander Heiden MG, Neveu JM, Lane WS, Cottrell JS, Horner JR, Cantley LC, Kalluri R, Asara JM. Biomolecular characterization and protein sequences of the Campanian hadrosaur B. canadensis. Science. 2009 May 1; 324(5927):626-31.
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  72. Karnchanaphanurach P, Mirchev R, Ghiran I, Asara JM, Papahadjopoulos-Sternberg B, Nicholson-Weller A, Golan DE. C3b deposition on human erythrocytes induces the formation of a membrane skeleton-linked protein complex. J Clin Invest. 2009 Apr; 119(4):788-801.
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  73. Zheng B, Jeong JH, Asara JM, Yuan YY, Granter SR, Chin L, Cantley LC. Oncogenic B-RAF negatively regulates the tumor suppressor LKB1 to promote melanoma cell proliferation. Mol Cell. 2009 Jan 30; 33(2):237-47.
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  74. Clements RT, Smejkal G, Sodha NR, Ivanov AR, Asara JM, Feng J, Lazarev A, Gautam S, Senthilnathan V, Khabbaz KR, Bianchi C, Sellke FW. Pilot proteomic profile of differentially regulated proteins in right atrial appendage before and after cardiac surgery using cardioplegia and cardiopulmonary bypass. Circulation. 2008 Sep 30; 118(14 Suppl):S24-31.
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  75. Organ CL, Schweitzer MH, Zheng W, Freimark LM, Cantley LC, Asara JM. Molecular phylogenetics of mastodon and Tyrannosaurus rex. Science. 2008 Apr 25; 320(5875):499.
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  76. Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC. Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature. 2008 Mar 13; 452(7184):181-6.
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  77. Asara JM, Christofk HR, Freimark LM, Cantley LC. A label-free quantification method by MS/MS TIC compared to SILAC and spectral counting in a proteomics screen. Proteomics. 2008 Mar; 8(5):994-9.
    View in: PubMed
  78. Zinkin NT, Grall F, Bhaskar K, Otu HH, Spentzos D, Kalmowitz B, Wells M, Guerrero M, Asara JM, Libermann TA, Afdhal NH. Serum proteomics and biomarkers in hepatocellular carcinoma and chronic liver disease. Clin Cancer Res. 2008 Jan 15; 14(2):470-7.
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  79. Asara JM, Garavelli JS, Slatter DA, Schweitzer MH, Freimark LM, Phillips M, Cantley LC. Interpreting sequences from mastodon and T. rex. Science. 2007 Sep 7; 317(5843):1324-5.
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  80. Hutti JE, Turk BE, Asara JM, Ma A, Cantley LC, Abbott DW. IkappaB kinase beta phosphorylates the K63 deubiquitinase A20 to cause feedback inhibition of the NF-kappaB pathway. Mol Cell Biol. 2007 Nov; 27(21):7451-61.
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  81. Asara JM, Schweitzer MH, Freimark LM, Phillips M, Cantley LC. Protein sequences from mastodon and Tyrannosaurus rex revealed by mass spectrometry. Science. 2007 Apr 13; 316(5822):280-5.
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  82. Schweitzer MH, Suo Z, Avci R, Asara JM, Allen MA, Arce FT, Horner JR. Analyses of soft tissue from Tyrannosaurus rex suggest the presence of protein. Science. 2007 Apr 13; 316(5822):277-80.
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  83. Asara JM, Zhang X, Zheng B, Christofk HH, Wu N, Cantley LC. In-Gel Stable-Isotope Labeling (ISIL): a strategy for mass spectrometry-based relative quantification. J Proteome Res. 2006 Jan; 5(1):155-63.
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  84. Asara JM, Zhang X, Zheng B, Maroney LA, Christofk HR, Wu N, Cantley LC. In-gel stable isotope labeling for relative quantification using mass spectrometry. Nat Protoc. 2006; 1(1):46-51.
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  85. Zhang X, Asara JM, Adamec J, Ouzzani M, Elmagarmid AK. Data pre-processing in liquid chromatography-mass spectrometry-based proteomics. Bioinformatics. 2005 Nov 1; 21(21):4054-9.
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  86. Zhang X, Hines W, Adamec J, Asara JM, Naylor S, Regnier FE. An automated method for the analysis of stable isotope labeling data in proteomics. J Am Soc Mass Spectrom. 2005 Jul; 16(7):1181-91.
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  87. Abbott DW, Wilkins A, Asara JM, Cantley LC. The Crohn's disease protein, NOD2, requires RIP2 in order to induce ubiquitinylation of a novel site on NEMO. Curr Biol. 2004 Dec 29; 14(24):2217-27.
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  88. Levine MZ, Calderón JC, Wilkins PP, Lane WS, Asara JM, Hancock K, Gonzalez AE, Garcia HH, Gilman RH, Tsang VC. Characterization, cloning, and expression of two diagnostic antigens for Taenia solium tapeworm infection. J Parasitol. 2004 Jun; 90(3):631-8.
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  89. Sano H, Kane S, Sano E, Mîinea CP, Asara JM, Lane WS, Garner CW, Lienhard GE. Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation. J Biol Chem. 2003 Apr 25; 278(17):14599-602.
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  90. Schweitzer M, Hill CL, Asara JM, Lane WS, Pincus SH. Identification of immunoreactive material in mammoth fossils. J Mol Evol. 2002 Dec; 55(6):696-705.
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  91. Kane S, Sano H, Liu SC, Asara JM, Lane WS, Garner CC, Lienhard GE. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain. J Biol Chem. 2002 Jun 21; 277(25):22115-8.
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  92. Ivan M, Kondo K, Yang H, Kim W, Valiando J, Ohh M, Salic A, Asara JM, Lane WS, Kaelin WG. HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Science. 2001 Apr 20; 292(5516):464-8.
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  93. Oh MH, Ray WK, Huber SC, Asara JM, Gage DA, Clouse SD. Recombinant brassinosteroid insensitive 1 receptor-like kinase autophosphorylates on serine and threonine residues and phosphorylates a conserved peptide motif in vitro. Plant Physiol. 2000 Oct; 124(2):751-66.
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  94. Asara JM, Allison J. Enhanced detection of oligonucleotides in UV MALDI MS using the tetraamine spermine as a matrix additive. Anal Chem. 1999 Jul 15; 71(14):2866-70.
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  95. Asara JM, Allison J. Enhanced detection of phosphopeptides in matrix-assisted laser desorption/ionization mass spectrometry using ammonium salts. J Am Soc Mass Spectrom. 1999 Jan; 10(1):35-44.
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