Contact, publication, and social network information about Harvard faculty and fellows. Harvard Catalyst Profiles
Keywords
Last Name
Institution

Bakhos A. Tannous, PH.D.

TitleAssociate Professor of Neurology
InstitutionMassachusetts General Hospital
DepartmentNeurology
AddressMassachusetts General Hospital
Room 6101
149 13th Street
Charlestown MA 02129
Phone617/726-6026
Fax617/724-1537

 Mentoring 
 current student opportunities
Available: 07/05/11, Expires: 07/01/15

Gliomas account for about 60% of all primary CNS tumors. Glioblastoma (GBM) or grade IV gliomas which comprise 50.9% of all gliomas are the most malignant form. Glioblastoma tumors are highly heterogeneous and there is a complex interaction among different types of tumor cells and stromal cells within the tumor. Recently it has been shown that the majority of tumor cells do not have the capacity to recapitulate a phenocopy of the original tumor and that only a small subpopulation of cells in the tumor, called cancer stem cells, have that ability upon xenotransplantation in nude mice. These cancer stem cells appear to be more resistant to conventional therapy, like chemotherapy and radiation, as compared to the non-cancer stem cells. Following current therapy for high-grade glioma tumors, most patients die within a year from a new secondary tumor foci forming within one centimeter of the resected area. These foci are enriched for cancer stem cells, and it is likely that they are responsible for tumor recurrence. Our proposal focuses on identifying small molecule drugs which can increase therapeutic efficacy for GBM stem cells. We plan to identity drug candidates which either kill primary GBM stem cells directly or can sensitize these cells to temozolomide, a commonly used chemotherapeutic agent for GBM. We will use a high throughput apoptosis-screening assay which we have developed based on the naturally secreted Gaussia luciferase and screen different small molecule libraries including ones containing FDA-approved drugs which crosses the blood-brain barrier. We will validate the most promising drug hits in our experimental glioma stem cells-bioluminescent model in vivo. Students would help the post-doctoral fellow in performing in culture as well as in vivo work related to this project.


 Research 
 research resources
This researcher has shared information about their research resources
in the eagle-i Network. To update or add resource records, contact
eagle-i@hms.harvard.edu.

 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Lewandrowski GK, Magee CN, Mounayar M, Tannous BA, Azzi J. Simultaneous in vivo monitoring of regulatory and effector T lymphocytes using secreted gaussia luciferase, firefly luciferase, and secreted alkaline phosphatase. Methods Mol Biol. 2014; 1098:211-27.
    View in: PubMed
  2. Degeling MH, Bovenberg MS, Tannous M, Tannous BA. Gaussia luciferase-based Mycoplasma detection assay in Mammalian cell culture. Methods Mol Biol. 2014; 1098:47-55.
    View in: PubMed
  3. Lai CP, Tannous BA, Breakefield XO. Noninvasive in vivo monitoring of extracellular vesicles. Methods Mol Biol. 2014; 1098:249-58.
    View in: PubMed
  4. Bovenberg MS, Degeling MH, Hejazi S, Amante RJ, van Keulen M, Jeuken JW, Akbaripanahi S, Vleggeert-Lankamp CL, Tannous M, Wesseling P, Wurdinger T, Tannous BA. Multiplex blood reporters for simultaneous monitoring of cellular processes. Anal Chem. 2013 Nov 5; 85(21):10205-10.
    View in: PubMed
  5. Tannous BA, Kerami M, Van der Stoop PM, Kwiatkowski N, Wang J, Zhou W, Kessler AF, Lewandrowski G, Hiddingh L, Sol N, Lagerweij T, Wedekind L, Niers JM, Barazas M, Nilsson RJ, Geerts D, De Witt Hamer PC, Hagemann C, Vandertop WP, Van Tellingen O, Noske DP, Gray NS, Würdinger T. Effects of the selective MPS1 inhibitor MPS1-IN-3 on glioblastoma sensitivity to antimitotic drugs. J Natl Cancer Inst. 2013 Sep 4; 105(17):1322-31.
    View in: PubMed
  6. Maguire CA, Crommentuijn MH, Mu D, Hudry E, Serrano-Pozo A, Hyman BT, Tannous BA. Mouse Gender Influences Brain Transduction by Intravascularly Administered AAV9. Mol Ther. 2013 Aug; 21(8):1470-1.
    View in: PubMed
  7. Maguire CA, Bovenberg MS, Crommentuijn MH, Niers JM, Kerami M, Teng J, Sena-Esteves M, Badr CE, Tannous BA. Triple bioluminescence imaging for in vivo monitoring of cellular processes. Mol Ther Nucleic Acids. 2013; 2:e99.
    View in: PubMed
  8. Bovenberg MS, Degeling MH, Tannous BA. Cell-based Immunotherapy Against Gliomas: From Bench to Bedside. Mol Ther. 2013 Jul; 21(7):1297-305.
    View in: PubMed
  9. Bovenberg MS, Degeling MH, Tannous BA. Advances in stem cell therapy against gliomas. Trends Mol Med. 2013 May; 19(5):281-91.
    View in: PubMed
  10. Badr CE, Van Hoppe S, Dumbuya H, Tjon-Kon-Fat LA, Tannous BA. Targeting cancer cells with the natural compound obtusaquinone. J Natl Cancer Inst. 2013 May 1; 105(9):643-53.
    View in: PubMed
  11. Degeling MH, Bovenberg MS, Lewandrowski GK, de Gooijer MC, Vleggeert-Lankamp CL, Tannous M, Maguire CA, Tannous BA. Directed molecular evolution reveals gaussia luciferase variants with enhanced light output stability. Anal Chem. 2013 Mar 5; 85(5):3006-12.
    View in: PubMed
  12. van Rijn S, Nilsson J, Noske DP, Vandertop WP, Tannous BA, Würdinger T. Functional multiplex reporter assay using tagged Gaussia luciferase. Sci Rep. 2013; 3:1046.
    View in: PubMed
  13. Pike LS, Tannous BA, Deliolanis NC, Hsich G, Morse D, Tung CH, Sena-Esteves M, Breakefield XO. Imaging gene delivery in a mouse model of congenital neuronal ceroid lipofuscinosis. Gene Ther. 2012 Nov; 19(11):1121.
    View in: PubMed
  14. Kodack DP, Chung E, Yamashita H, Incio J, Duyverman AM, Song Y, Farrar CT, Huang Y, Ager E, Kamoun W, Goel S, Snuderl M, Lussiez A, Hiddingh L, Mahmood S, Tannous BA, Eichler AF, Fukumura D, Engelman JA, Jain RK. Combined targeting of HER2 and VEGFR2 for effective treatment of HER2-amplified breast cancer brain metastases. Proc Natl Acad Sci U S A. 2012 Nov 6; 109(45):E3119-27.
    View in: PubMed
  15. Degeling MH, Maguire CA, Bovenberg MS, Tannous BA. Sensitive assay for mycoplasma detection in mammalian cell culture. Anal Chem. 2012 May 1; 84(9):4227-32.
    View in: PubMed
  16. Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, Ahn HJ, Ait-Mohamed O, Ait-Si-Ali S, Akematsu T, Akira S, Al-Younes HM, Al-Zeer MA, Albert ML, Albin RL, Alegre-Abarrategui J, Aleo MF, Alirezaei M, Almasan A, Almonte-Becerril M, Amano A, Amaravadi R, Amarnath S, Amer AO, Andrieu-Abadie N, Anantharam V, Ann DK, Anoopkumar-Dukie S, Aoki H, Apostolova N, Arancia G, Aris JP, Asanuma K, Asare NY, Ashida H, Askanas V, Askew DS, Auberger P, Baba M, Backues SK, Baehrecke EH, Bahr BA, Bai XY, Bailly Y, Baiocchi R, Baldini G, Balduini W, Ballabio A, Bamber BA, Bampton ET, Bánhegyi G, Bartholomew CR, Bassham DC, Bast RC, Batoko H, Bay BH, Beau I, Béchet DM, Begley TJ, Behl C, Behrends C, Bekri S, Bellaire B, Bendall LJ, Benetti L, Berliocchi L, Bernardi H, Bernassola F, Besteiro S, Bhatia-Kissova I, Bi X, Biard-Piechaczyk M, Blum JS, Boise LH, Bonaldo P, Boone DL, Bornhauser BC, Bortoluci KR, Bossis I, Bost F, Bourquin JP, Boya P, Boyer-Guittaut M, Bozhkov PV, Brady NR, Brancolini C, Brech A, Brenman JE, Brennand A, Bresnick EH, Brest P, Bridges D, Bristol ML, Brookes PS, Brown EJ, Brumell JH, Brunetti-Pierri N, Brunk UT, Bulman DE, Bultman SJ, Bultynck G, Burbulla LF, Bursch W, Butchar JP, Buzgariu W, Bydlowski SP, Cadwell K, Cahová M, Cai D, Cai J, Cai Q, Calabretta B, Calvo-Garrido J, Camougrand N, Campanella M, Campos-Salinas J, Candi E, Cao L, Caplan AB, Carding SR, Cardoso SM, Carew JS, Carlin CR, Carmignac V, Carneiro LA, Carra S, Caruso RA, Casari G, Casas C, Castino R, Cebollero E, Cecconi F, Celli J, Chaachouay H, Chae HJ, Chai CY, Chan DC, Chan EY, Chang RC, Che CM, Chen CC, Chen GC, Chen GQ, Chen M, Chen Q, Chen SS, Chen W, Chen X, Chen X, Chen X, Chen YG, Chen Y, Chen Y, Chen YJ, Chen Z, Cheng A, Cheng CH, Cheng Y, Cheong H, Cheong JH, Cherry S, Chess-Williams R, Cheung ZH, Chevet E, Chiang HL, Chiarelli R, Chiba T, Chin LS, Chiou SH, Chisari FV, Cho CH, Cho DH, Choi AM, Choi D, Choi KS, Choi ME, Chouaib S, Choubey D, Choubey V, Chu CT, Chuang TH, Chueh SH, Chun T, Chwae YJ, Chye ML, Ciarcia R, Ciriolo MR, Clague MJ, Clark RS, Clarke PG, Clarke R, Codogno P, Coller HA, Colombo MI, Comincini S, Condello M, Condorelli F, Cookson MR, Coombs GH, Coppens I, Corbalan R, Cossart P, Costelli P, Costes S, Coto-Montes A, Couve E, Coxon FP, Cregg JM, Crespo JL, Cronjé MJ, Cuervo AM, Cullen JJ, Czaja MJ, D'Amelio M, Darfeuille-Michaud A, Davids LM, Davies FE, De Felici M, de Groot JF, de Haan CA, De Martino L, De Milito A, De Tata V, Debnath J, Degterev A, Dehay B, Delbridge LM, Demarchi F, Deng YZ, Dengjel J, Dent P, Denton D, Deretic V, Desai SD, Devenish RJ, Di Gioacchino M, Di Paolo G, Di Pietro C, Díaz-Araya G, Díaz-Laviada I, Diaz-Meco MT, Diaz-Nido J, Dikic I, Dinesh-Kumar SP, Ding WX, Distelhorst CW, Diwan A, Djavaheri-Mergny M, Dokudovskaya S, Dong Z, Dorsey FC, Dosenko V, Dowling JJ, Doxsey S, Dreux M, Drew ME, Duan Q, Duchosal MA, Duff K, Dugail I, Durbeej M, Duszenko M, Edelstein CL, Edinger AL, Egea G, Eichinger L, Eissa NT, Ekmekcioglu S, El-Deiry WS, Elazar Z, Elgendy M, Ellerby LM, Eng KE, Engelbrecht AM, Engelender S, Erenpreisa J, Escalante R, Esclatine A, Eskelinen EL, Espert L, Espina V, Fan H, Fan J, Fan QW, Fan Z, Fang S, Fang Y, Fanto M, Fanzani A, Farkas T, Farré JC, Faure M, Fechheimer M, Feng CG, Feng J, Feng Q, Feng Y, Fésüs L, Feuer R, Figueiredo-Pereira ME, Fimia GM, Fingar DC, Finkbeiner S, Finkel T, Finley KD, Fiorito F, Fisher EA, Fisher PB, Flajolet M, Florez-McClure ML, Florio S, Fon EA, Fornai F, Fortunato F, Fotedar R, Fowler DH, Fox HS, Franco R, Frankel LB, Fransen M, Fuentes JM, Fueyo J, Fujii J, Fujisaki K, Fujita E, Fukuda M, Furukawa RH, Gaestel M, Gailly P, Gajewska M, Galliot B, Galy V, Ganesh S, Ganetzky B, Ganley IG, Gao FB, Gao GF, Gao J, Garcia L, Garcia-Manero G, Garcia-Marcos M, Garmyn M, Gartel AL, Gatti E, Gautel M, Gawriluk TR, Gegg ME, Geng J, Germain M, Gestwicki JE, Gewirtz DA, Ghavami S, et al. Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 2012 Apr; 8(4):445-544.
    View in: PubMed
  17. Morse D, Tannous BA. A water-soluble coelenterazine for sensitive in vivo imaging of coelenterate luciferases. Mol Ther. 2012 Apr; 20(4):692-3.
    View in: PubMed
  18. Niers JM, Chen JW, Lewandrowski G, Kerami M, Garanger E, Wojtkiewicz G, Waterman P, Keliher E, Weissleder R, Tannous BA. Single reporter for targeted multimodal in vivo imaging. J Am Chem Soc. 2012 Mar 21; 134(11):5149-56.
    View in: PubMed
  19. Maguire CA, Balaj L, Sivaraman S, Crommentuijn MH, Ericsson M, Mincheva-Nilsson L, Baranov V, Gianni D, Tannous BA, Sena-Esteves M, Breakefield XO, Skog J. Microvesicle-associated AAV vector as a novel gene delivery system. Mol Ther. 2012 May; 20(5):960-71.
    View in: PubMed
  20. Maguire CA, van der Mijn JC, Degeling MH, Morse D, Tannous BA. Codon-optimized Luciola italica luciferase variants for mammalian gene expression in culture and in vivo. Mol Imaging. 2012 Feb; 11(1):13-21.
    View in: PubMed
  21. Bovenberg MS, Degeling MH, Tannous BA. Enhanced Gaussia luciferase blood assay for monitoring of in vivo biological processes. Anal Chem. 2012 Jan 17; 84(2):1189-92.
    View in: PubMed
  22. Pike LS, Tannous BA, Deliolanis NC, Hsich G, Morse D, Tung CH, Sena-Esteves M, Breakefield XO. Imaging gene delivery in a mouse model of congenital neuronal ceroid lipofuscinosis. Gene Ther. 2011 Dec; 18(12):1173-8.
    View in: PubMed
  23. Tannous BA, Teng J. Secreted blood reporters: insights and applications. Biotechnol Adv. 2011 Nov-Dec; 29(6):997-1003.
    View in: PubMed
  24. Badr CE, Tannous BA. Bioluminescence imaging: progress and applications. Trends Biotechnol. 2011 Dec; 29(12):624-33.
    View in: PubMed
  25. Badr CE, Wurdinger T, Nilsson J, Niers JM, Whalen M, Degterev A, Tannous BA. Lanatoside C sensitizes glioblastoma cells to tumor necrosis factor-related apoptosis-inducing ligand and induces an alternative cell death pathway. Neuro Oncol. 2011 Nov; 13(11):1213-24.
    View in: PubMed
  26. Nery FC, Armata IA, Farley JE, Cho JA, Yaqub U, Chen P, da Hora CC, Wang Q, Tagaya M, Klein C, Tannous B, Caldwell KA, Caldwell GA, Lencer WI, Ye Y, Breakefield XO. TorsinA participates in endoplasmic reticulum-associated degradation. Nat Commun. 2011; 2:393.
    View in: PubMed
  27. Gabriely G, Yi M, Narayan RS, Niers JM, Wurdinger T, Imitola J, Ligon KL, Kesari S, Esau C, Stephens RM, Tannous BA, Krichevsky AM. Human glioma growth is controlled by microRNA-10b. Cancer Res. 2011 May 15; 71(10):3563-72.
    View in: PubMed
  28. Deliolanis NC, Wurdinger T, Pike L, Tannous BA, Breakefield XO, Weissleder R, Ntziachristos V. In vivo tomographic imaging of red-shifted fluorescent proteins. Biomed Opt Express. 2011; 2(4):887-900.
    View in: PubMed
  29. Niers JM, Kerami M, Pike L, Lewandrowski G, Tannous BA. Multimodal in vivo imaging and blood monitoring of intrinsic and extrinsic apoptosis. Mol Ther. 2011 Jun; 19(6):1090-6.
    View in: PubMed
  30. Smits M, Mir SE, Nilsson RJ, van der Stoop PM, Niers JM, Marquez VE, Cloos J, Breakefield XO, Krichevsky AM, Noske DP, Tannous BA, Würdinger T. Down-regulation of miR-101 in endothelial cells promotes blood vessel formation through reduced repression of EZH2. PLoS One. 2011; 6(1):e16282.
    View in: PubMed
  31. Niers JM, Chen JW, Weissleder R, Tannous BA. Enhanced in vivo imaging of metabolically biotinylated cell surface reporters. Anal Chem. 2011 Feb 1; 83(3):994-9.
    View in: PubMed
  32. Sharma R, Tsuchiya M, Tannous BA, Bartlett JD. Measurement of fluoride-induced endoplasmic reticulum stress using Gaussia luciferase. Methods Enzymol. 2011; 491:111-25.
    View in: PubMed
  33. Badr CE, Wurdinger T, Tannous BA. Functional drug screening assay reveals potential glioma therapeutics. Assay Drug Dev Technol. 2011 Jun; 9(3):281-9.
    View in: PubMed
  34. Badr CE, Niers JM, Morse D, Koelen JA, Vandertop P, Noske D, Wurdinger T, Zalloua PA, Tannous BA. Suicidal gene therapy in an NF-?B-controlled tumor environment as monitored by a secreted blood reporter. Gene Ther. 2011 May; 18(5):445-51.
    View in: PubMed
  35. Smits M, Nilsson J, Mir SE, van der Stoop PM, Hulleman E, Niers JM, de Witt Hamer PC, Marquez VE, Cloos J, Krichevsky AM, Noske DP, Tannous BA, Würdinger T. miR-101 is down-regulated in glioblastoma resulting in EZH2-induced proliferation, migration, and angiogenesis. Oncotarget. 2010 Dec; 1(8):710-20.
    View in: PubMed
  36. Mir SE, De Witt Hamer PC, Krawczyk PM, Balaj L, Claes A, Niers JM, Van Tilborg AA, Zwinderman AH, Geerts D, Kaspers GJ, Peter Vandertop W, Cloos J, Tannous BA, Wesseling P, Aten JA, Noske DP, Van Noorden CJ, Würdinger T. In silico analysis of kinase expression identifies WEE1 as a gatekeeper against mitotic catastrophe in glioblastoma. Cancer Cell. 2010 Sep 14; 18(3):244-57.
    View in: PubMed
  37. Sharma R, Tsuchiya M, Skobe Z, Tannous BA, Bartlett JD. The acid test of fluoride: how pH modulates toxicity. PLoS One. 2010; 5(5):e10895.
    View in: PubMed
  38. Chung E, Yamashita H, Au P, Tannous BA, Fukumura D, Jain RK. Secreted Gaussia luciferase as a biomarker for monitoring tumor progression and treatment response of systemic metastases. PLoS One. 2009; 4(12):e8316.
    View in: PubMed
  39. Prabhakar S, Brenner GJ, Sung B, Messerli SM, Mao J, Sena-Esteves M, Stemmer-Rachamimov A, Tannous B, Breakefield XO. Imaging and therapy of experimental schwannomas using HSV amplicon vector-encoding apoptotic protein under Schwann cell promoter. Cancer Gene Ther. 2010 Apr; 17(4):266-74.
    View in: PubMed
  40. Badr CE, Niers JM, Tjon-Kon-Fat LA, Noske DP, Wurdinger T, Tannous BA. Real-time monitoring of nuclear factor kappaB activity in cultured cells and in animal models. Mol Imaging. 2009 Sep-Oct; 8(5):278-90.
    View in: PubMed
  41. Saydam O, Shen Y, Würdinger T, Senol O, Boke E, James MF, Tannous BA, Stemmer-Rachamimov AO, Yi M, Stephens RM, Fraefel C, Gusella JF, Krichevsky AM, Breakefield XO. Downregulated microRNA-200a in meningiomas promotes tumor growth by reducing E-cadherin and activating the Wnt/beta-catenin signaling pathway. Mol Cell Biol. 2009 Nov; 29(21):5923-40.
    View in: PubMed
  42. Maguire CA, Deliolanis NC, Pike L, Niers JM, Tjon-Kon-Fat LA, Sena-Esteves M, Tannous BA. Gaussia luciferase variant for high-throughput functional screening applications. Anal Chem. 2009 Aug 15; 81(16):7102-6.
    View in: PubMed
  43. van Vuurden DG, Yazdani M, Bosma I, Broekhuizen AJ, Postma TJ, Heimans JJ, van der Valk P, Aronica E, Tannous BA, Würdinger T, Kaspers GJ, Cloos J. Attenuated AMPA receptor expression allows glioblastoma cell survival in glutamate-rich environment. PLoS One. 2009; 4(6):e5953.
    View in: PubMed
  44. Deliolanis NC, Dunham J, Wurdinger T, Figueiredo JL, Tannous BA, Bakhos T, Ntziachristos V. In-vivo imaging of murine tumors using complete-angle projection fluorescence molecular tomography. J Biomed Opt. 2009 May-Jun; 14(3):030509.
    View in: PubMed
  45. Würdinger T, Tannous BA. Glioma angiogenesis: Towards novel RNA therapeutics. Cell Adh Migr. 2009 Apr-Jun; 3(2):230-5.
    View in: PubMed
  46. Tannous BA, Christensen AP, Pike L, Wurdinger T, Perry KF, Saydam O, Jacobs AH, García-Añoveros J, Weissleder R, Sena-Esteves M, Corey DP, Breakefield XO. Mutant sodium channel for tumor therapy. Mol Ther. 2009 May; 17(5):810-9.
    View in: PubMed
  47. Tannous BA. Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. Nat Protoc. 2009; 4(4):582-91.
    View in: PubMed
  48. Würdinger T, Tannous BA, Saydam O, Skog J, Grau S, Soutschek J, Weissleder R, Breakefield XO, Krichevsky AM. miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cells. Cancer Cell. 2008 Nov 4; 14(5):382-93.
    View in: PubMed
  49. Tannous B, Lee-Lewandrowski E, Sharples C, Brugge W, Bigatello L, Thompson T, Benzer T, Lewandrowski K. Comparison of conventional guaiac to four immunochemical methods for fecal occult blood testing: implications for clinical practice in hospital and outpatient settings. Clin Chim Acta. 2009 Feb; 400(1-2):120-2.
    View in: PubMed
  50. Deliolanis NC, Kasmieh R, Wurdinger T, Tannous BA, Shah K, Ntziachristos V. Performance of the red-shifted fluorescent proteins in deep-tissue molecular imaging applications. J Biomed Opt. 2008 Jul-Aug; 13(4):044008.
    View in: PubMed
  51. Lewandrowski K, Flood J, Finn C, Tannous B, Farris AB, Benzer TI, Lee-Lewandrowski E. Implementation of point-of-care rapid urine testing for drugs of abuse in the emergency department of an academic medical center: impact on test utilization and ED length of stay. Am J Clin Pathol. 2008 May; 129(5):796-801.
    View in: PubMed
  52. Hewett JW, Nery FC, Niland B, Ge P, Tan P, Hadwiger P, Tannous BA, Sah DW, Breakefield XO. siRNA knock-down of mutant torsinA restores processing through secretory pathway in DYT1 dystonia cells. Hum Mol Genet. 2008 May 15; 17(10):1436-45.
    View in: PubMed
  53. Wurdinger T, Badr C, Pike L, de Kleine R, Weissleder R, Breakefield XO, Tannous BA. A secreted luciferase for ex vivo monitoring of in vivo processes. Nat Methods. 2008 Feb; 5(2):171-3.
    View in: PubMed
  54. Lee-Lewandrowski E, Januzzi JL, Green SM, Tannous B, Wu AH, Smith A, Wong A, Murakami MM, Kaczmarek J, Apple FS, Miller WL, Hartman K, Jaffe AS. Multi-center validation of the Response Biomedical Corporation RAMP NT-proBNP assay with comparison to the Roche Diagnostics GmbH Elecsys proBNP assay. Clin Chim Acta. 2007 Nov-Dec; 386(1-2):20-4.
    View in: PubMed
  55. Badr CE, Hewett JW, Breakefield XO, Tannous BA. A highly sensitive assay for monitoring the secretory pathway and ER stress. PLoS One. 2007; 2(6):e571.
    View in: PubMed
  56. Hewett JW, Tannous B, Niland BP, Nery FC, Zeng J, Li Y, Breakefield XO. Mutant torsinA interferes with protein processing through the secretory pathway in DYT1 dystonia cells. Proc Natl Acad Sci U S A. 2007 Apr 24; 104(17):7271-6.
    View in: PubMed
  57. Niland B, Hewett J, Tannous BA and Breakefield XO. metabolic biotinylation of TorsinA reveals intracellular fate (in preparation). 2006.
  58. Wurdinger T, Weissleder R, Breakefield XO and Tannous BA. Gaussia luciferase for monitoring of tumor volume in vivo by blood sampling (in preparation). 2006.
  59. Badr C, Zallou P, Breakefield XO and Tannous BA. Novel ionizing-radiation inducible system for tumor gene therapy (in preparation). 2006.
  60. Christian B, Hewett J, Breakefield XO and Tannous BA. Gaussia luciferase for monitoring of secretory pathway and ER stress (in preparation). 2006.
  61. Tannous BA. Metabolic biotinylation is cell dependent and not efficient in mammalian cells (in preparation). 2006.
  62. Hewett J, Tannous BA, Niland B and Breakefield XO. Mutant torsinA interfers with protein processing through the secretory pathway in DYT1 dystonia cells (submitted to PNAS). 2006.
  63. Tannous BA, Hewett J, Breakefield XO. A versatile, highly sensitive assay for monitoring of protein secretion, promoter activity and cell viability. 2006.
  64. Tannous BA, Grimm J, Perry KF, Chen JW, Weissleder R, Breakefield XO. Metabolic biotinylation of cell surface receptors for in vivo imaging. Nat Methods. 2006 May; 3(5):391-6.
    View in: PubMed
  65. Badr C, Zalloua P, Breakefield XO, Tannous BA . Imaging of radiation-inducible promoters using a naturally secreted luciferase from the marine copepod Gaussia princeps. Mol Therapy. 2006; 13(S1):S369.
  66. Tannous BA, Perry KF, Sena-Esteves M, Christensen A, Garcia-Anoveros J, Jacobs A, Corey D, Weissleder R, Breakefield XO . Tumor therapy via vector-regulated expression of mutant sodium channel. Mol Therapy. 2006; 13(S1):S370.
  67. Tannous BA, Grimm J, Perry K, Weissleder R, Breakefield XO . Metabolic biotinylation of cell surface receptors for imaging. Mol. Therapy. 2006; 13(S1):S386.
  68. Tannous BA, Badr C, Weissleder R, Breakefield XO, Zalloua P . Ionizing radiation-inducible system for imaging of gene expression. Mol Imaging. 2006; 5:384.
  69. Tannous BA, Perry K, Sena-Esteves M, Garcia-Anoveros J, Jacobs A, Corey D, Weissleder R, Breakefield XO . Mutant Sodium Channel for Tumor Therapy. Mol Imaging. 2006; 5:341.
  70. Tannous BA, Grimm J, Perry KF, Weissleder R, Breakefield XO. Metabolic biotinylation of mammalian cell receptors for imaging. Nat Protocols. 2006; 10.1038:132.
  71. Wurdinger T, Badr C, Weissleder R, Breakefield XO, Tannous BA. Novel construct for imaging of NF-êB activation using bioluminescence. Mol Imaging. 2006; 5:384.
  72. Tannous BA, Kim DE, Fernandez JL, Weissleder R, Breakefield XO. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther. 2005 Mar; 11(3):435-43.
    View in: PubMed
  73. Emmanouilidou E, Tannous B, Ioannou PC, Christopoulos TK. Duplex RT-PCR and chemiluminometric hybridization assay for combined screening of the mRNAs of prostate-specific antigen and prostate-specific membrane antigen in peripheral blood. Anal Chim Acta. 2005; 531(2):193-198.
  74. Breakefield XO, Tannous BA, Shah K, Grimm J, Tang Y, Tung C, Weissleder R. Imaging fate and action of gene and stem cell-based therapies in the central nervous system. Mol Imaging. 2005; 4:217.
  75. Tannous BA, Verhaegen M, Christopoulos TK, Kourakli A. Combined flash- and glow-type chemiluminescent reactions for high-throughput genotyping of biallelic polymorphisms. Anal Biochem. 2003 Sep 15; 320(2):266-72.
    View in: PubMed
  76. Tannous BA, Laios E, Christopoulos TK. T7 RNA polymerase as a self-replicating label for antigen quantification. Nucleic Acids Res. 2002 Dec 15; 30(24):e140.
    View in: PubMed
  77. Tannous BA, Chiu NHL, Christopoulos TK. Heterobifunctional linker between antibodies and reporter genes for immunoassay development. Analytica Chimica Acta. 2002; 459(2):169-176.
  78. Tannous BA. Novel methods for antigens and nucleic acids analysis. 2002.
Local representatives can answer questions about the Profiles website or help with editing a profile or issues with profile data. For assistance with this profile: HMS/HSDM faculty should contact Human Resources at faculty_serviceshms.harvard.edu.
Tannous's Networks
Click the "See All" links for more information and interactive visualizations!
Concepts
_
Co-Authors
_
Similar People
_
Same Department
Physical Neighbors
_