 Ram Sasisekharan, PH.D.
| Title | Co-Director of the Harvard-MIT Division of Health Sciences and Technology |
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| Institution | Massachusetts Institute of Technology |
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| Department | Health Sciences and Technology |
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| Address | Mass Institute Of Technology
E25-519
77 Massachusetts Ave
Cambridge MA 02139
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| Phone | 617/258-9494 |
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| Fax | 617/253-7498 |
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 Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications.
Faculty can login to make corrections and additions.
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Srinivasan K, Raman R, Jayaraman A, Viswanathan K, Sasisekharan R. Quantitative description of glycan-receptor binding of influenza a virus h7 hemagglutinin. PLoS One. 2013; 8(2):e49597.
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Jayaraman A, Koh X, Li J, Raman R, Viswanathan K, Shriver Z, Sasisekharan R. Glycosylation at Asn91 of H1N1 haemagglutinin affects binding to glycan receptors. Biochem J. 2012 Jun 15; 444(3):429-35.
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Robinson LN, Artpradit C, Raman R, Shriver ZH, Ruchirawat M, Sasisekharan R. Harnessing glycomics technologies: integrating structure with function for glycan characterization. Electrophoresis. 2012 Mar; 33(5):797-814.
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Pearce MB, Jayaraman A, Pappas C, Belser JA, Zeng H, Gustin KM, Maines TR, Sun X, Raman R, Cox NJ, Sasisekharan R, Katz JM, Tumpey TM. Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets. Proc Natl Acad Sci U S A. 2012 Mar 6; 109(10):3944-9.
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Jayaraman A, Chandrasekaran A, Viswanathan K, Raman R, Fox JG, Sasisekharan R. Decoding the distribution of glycan receptors for human-adapted influenza A viruses in ferret respiratory tract. PLoS One. 2012; 7(2):e27517.
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Morris MK, Shriver Z, Sasisekharan R, Lauffenburger DA. Querying quantitative logic models (Q2LM) to study intracellular signaling networks and cell-cytokine interactions. Biotechnol J. 2012 Mar; 7(3):374-86.
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Shriver Z, Capila I, Venkataraman G, Sasisekharan R. Heparin and heparan sulfate: analyzing structure and microheterogeneity. Handb Exp Pharmacol. 2012; (207):159-76.
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Soundararajan V, Zheng S, Patel N, Warnock K, Raman R, Wilson IA, Raguram S, Sasisekharan V, Sasisekharan R. Networks link antigenic and receptor-binding sites of influenza hemagglutinin: mechanistic insight into fitter strain propagation. Sci Rep. 2011; 1:200.
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Belser JA, Jayaraman A, Raman R, Pappas C, Zeng H, Cox NJ, Katz JM, Sasisekharan R, Tumpey TM. Effect of D222G mutation in the hemagglutinin protein on receptor binding, pathogenesis and transmissibility of the 2009 pandemic H1N1 influenza virus. PLoS One. 2011; 6(9):e25091.
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Aich U, Shriver Z, Tharakaraman K, Raman R, Sasisekharan R. Competitive inhibition of heparinase by persulfonated glycosaminoglycans: a tool to detect heparin contamination. Anal Chem. 2011 Oct 15; 83(20):7815-22.
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Aich U, Beckley N, Shriver Z, Raman R, Viswanathan K, Hobbie S, Sasisekharan R. Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay. FEBS J. 2011 May; 278(10):1699-712.
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Maines TR, Chen LM, Van Hoeven N, Tumpey TM, Blixt O, Belser JA, Gustin KM, Pearce MB, Pappas C, Stevens J, Cox NJ, Paulson JC, Raman R, Sasisekharan R, Katz JM, Donis RO. Effect of receptor binding domain mutations on receptor binding and transmissibility of avian influenza H5N1 viruses. Virology. 2011 Apr 25; 413(1):139-47.
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Jayaraman A, Pappas C, Raman R, Belser JA, Viswanathan K, Shriver Z, Tumpey TM, Sasisekharan R. A single base-pair change in 2009 H1N1 hemagglutinin increases human receptor affinity and leads to efficient airborne viral transmission in ferrets. PLoS One. 2011; 6(3):e17616.
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Bosques CJ, Collins BE, Meador JW, Sarvaiya H, Murphy JL, Dellorusso G, Bulik DA, Hsu IH, Washburn N, Sipsey SF, Myette JR, Raman R, Shriver Z, Sasisekharan R, Venkataraman G. Chinese hamster ovary cells can produce galactose-a-1,3-galactose antigens on proteins. Nat Biotechnol. 2010 Nov; 28(11):1153-6.
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Viswanathan K, Koh X, Chandrasekaran A, Pappas C, Raman R, Srinivasan A, Shriver Z, Tumpey TM, Sasisekharan R. Determinants of glycan receptor specificity of H2N2 influenza A virus hemagglutinin. PLoS One. 2010; 5(10):e13768.
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Eavarone DA, Soundararajan V, Haller T, Sasisekharan R. A voxel-based Monte Carlo model of drug release from bulk eroding nanoparticles. J Nanosci Nanotechnol. 2010 Sep; 10(9):5903-7.
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Viswanathan K, Chandrasekaran A, Srinivasan A, Raman R, Sasisekharan V, Sasisekharan R. Glycans as receptors for influenza pathogenesis. Glycoconj J. 2010 Aug; 27(6):561-70.
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Soundararajan V, Patel N, Subramanian V, Sasisekharan V, Sasisekharan R. The many faces of the YopM effector from plague causative bacterium Yersinia pestis and its implications for host immune modulation. Innate Immun. 2011 Dec; 17(6):548-57.
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Turnbull JE, Sasisekharan R. Glycomics: technologies taming a frontier omics field. OMICS. 2010 Aug; 14(4):385-7.
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Pappas C, Viswanathan K, Chandrasekaran A, Raman R, Katz JM, Sasisekharan R, Tumpey TM. Receptor specificity and transmission of H2N2 subtype viruses isolated from the pandemic of 1957. PLoS One. 2010; 5(6):e11158.
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Soundararajan V, Raman R, Raguram S, Sasisekharan V, Sasisekharan R. Atomic interaction networks in the core of protein domains and their native folds. PLoS One. 2010; 5(2):e9391.
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Soundararajan V, Warnock K, Sasisekharan R. Multifunctional nanoscale platforms for targeting of the cancer cell immortality spectrum. Macromol Rapid Commun. 2010 Jan 18; 31(2):202-16.
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Sasisekharan R, Shriver Z. From crisis to opportunity: a perspective on the heparin crisis. Thromb Haemost. 2009 Nov; 102(5):854-8.
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Kishimoto TK, Qi YW, Long A, Capila I, Sasisekharan R, Guerrero L, Fier I, Roach J, Venkataraman G. M118--a rationally engineered low-molecular-weight heparin designed specifically for the treatment of acute coronary syndromes. Thromb Haemost. 2009 Nov; 102(5):900-6.
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Guerrini M, Shriver Z, Bisio A, Naggi A, Casu B, Sasisekharan R, Torri G. The tainted heparin story: an update. Thromb Haemost. 2009 Nov; 102(5):907-11.
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Bisio A, Vecchietti D, Citterio L, Guerrini M, Raman R, Bertini S, Eisele G, Naggi A, Sasisekharan R, Torri G. Structural features of low-molecular-weight heparins affecting their affinity to antithrombin. Thromb Haemost. 2009 Nov; 102(5):865-73.
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Hensley SE, Das SR, Bailey AL, Schmidt LM, Hickman HD, Jayaraman A, Viswanathan K, Raman R, Sasisekharan R, Bennink JR, Yewdell JW. Hemagglutinin receptor binding avidity drives influenza A virus antigenic drift. Science. 2009 Oct 30; 326(5953):734-6.
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Harfouche R, Hentschel DM, Piecewicz S, Basu S, Print C, Eavarone D, Kiziltepe T, Sasisekharan R, Sengupta S. Glycome and transcriptome regulation of vasculogenesis. Circulation. 2009 Nov 10; 120(19):1883-92.
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Guerrini M, Zhang Z, Shriver Z, Naggi A, Masuko S, Langer R, Casu B, Linhardt RJ, Torri G, Sasisekharan R. Orthogonal analytical approaches to detect potential contaminants in heparin. Proc Natl Acad Sci U S A. 2009 Oct 6; 106(40):16956-61.
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Myette JR, Soundararajan V, Behr J, Shriver Z, Raman R, Sasisekharan R. Heparin/heparan sulfate N-sulfamidase from Flavobacterium heparinum: structural and biochemical investigation of catalytic nitrogen-sulfur bond cleavage. J Biol Chem. 2009 Dec 11; 284(50):35189-200.
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Myette JR, Soundararajan V, Shriver Z, Raman R, Sasisekharan R. Heparin/heparan sulfate 6-O-sulfatase from Flavobacterium heparinum: integrated structural and biochemical investigation of enzyme active site and substrate specificity. J Biol Chem. 2009 Dec 11; 284(50):35177-88.
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Shriver Z, Raman R, Viswanathan K, Sasisekharan R. Context-specific target definition in influenza a virus hemagglutinin-glycan receptor interactions. Chem Biol. 2009 Aug 28; 16(8):803-14.
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Maines TR, Jayaraman A, Belser JA, Wadford DA, Pappas C, Zeng H, Gustin KM, Pearce MB, Viswanathan K, Shriver ZH, Raman R, Cox NJ, Sasisekharan R, Katz JM, Tumpey TM. Transmission and pathogenesis of swine-origin 2009 A(H1N1) influenza viruses in ferrets and mice. Science. 2009 Jul 24; 325(5939):484-7.
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Bulmer MS, Bachelet I, Raman R, Rosengaus RB, Sasisekharan R. Targeting an antimicrobial effector function in insect immunity as a pest control strategy. Proc Natl Acad Sci U S A. 2009 Aug 4; 106(31):12652-7.
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Soundararajan V, Tharakaraman K, Raman R, Raguram S, Shriver Z, Sasisekharan V, Sasisekharan R. Extrapolating from sequence--the 2009 H1N1 'swine' influenza virus. Nat Biotechnol. 2009 Jun; 27(6):510-3.
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Van Hoeven N, Pappas C, Belser JA, Maines TR, Zeng H, García-Sastre A, Sasisekharan R, Katz JM, Tumpey TM. Human HA and polymerase subunit PB2 proteins confer transmission of an avian influenza virus through the air. Proc Natl Acad Sci U S A. 2009 Mar 3; 106(9):3366-71.
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Prabhakar V, Capila I, Sasisekharan R. Glycosaminoglycan characterization methodologies: probing biomolecular interactions. Methods Mol Biol. 2009; 534:331-40.
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Prabhakar V, Capila I, Sasisekharan R. The structural elucidation of glycosaminoglycans. Methods Mol Biol. 2009; 534:147-56.
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Guglier S, Hricovíni M, Raman R, Polito L, Torri G, Casu B, Sasisekharan R, Guerrini M. Minimum FGF2 binding structural requirements of heparin and heparan sulfate oligosaccharides as determined by NMR spectroscopy. Biochemistry. 2008 Dec 30; 47(52):13862-9.
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Blossom DB, Kallen AJ, Patel PR, Elward A, Robinson L, Gao G, Langer R, Perkins KM, Jaeger JL, Kurkjian KM, Jones M, Schillie SF, Shehab N, Ketterer D, Venkataraman G, Kishimoto TK, Shriver Z, McMahon AW, Austen KF, Kozlowski S, Srinivasan A, Turabelidze G, Gould CV, Arduino MJ, Sasisekharan R. Outbreak of adverse reactions associated with contaminated heparin. N Engl J Med. 2008 Dec 18; 359(25):2674-84.
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Ban Z, Bosques CJ, Sasisekharan R. A simple assay to probe disease-associated enzyme activity using glycosaminoglycan-assisted synthesized gold nanoparticles. Org Biomol Chem. 2008 Dec 7; 6(23):4290-2.
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Prabhakar V, Capila I, Soundararajan V, Raman R, Sasisekharan R. Recombinant expression, purification, and biochemical characterization of chondroitinase ABC II from Proteus vulgaris. J Biol Chem. 2009 Jan 9; 284(2):974-82.
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Kishimoto TK, Viswanathan K, Ganguly T, Elankumaran S, Smith S, Pelzer K, Lansing JC, Sriranganathan N, Zhao G, Galcheva-Gargova Z, Al-Hakim A, Bailey GS, Fraser B, Roy S, Rogers-Cotrone T, Buhse L, Whary M, Fox J, Nasr M, Dal Pan GJ, Shriver Z, Langer RS, Venkataraman G, Austen KF, Woodcock J, Sasisekharan R. Contaminated heparin associated with adverse clinical events and activation of the contact system. N Engl J Med. 2008 Jun 5; 358(23):2457-67.
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Guerrini M, Beccati D, Shriver Z, Naggi A, Viswanathan K, Bisio A, Capila I, Lansing JC, Guglieri S, Fraser B, Al-Hakim A, Gunay NS, Zhang Z, Robinson L, Buhse L, Nasr M, Woodcock J, Langer R, Venkataraman G, Linhardt RJ, Casu B, Torri G, Sasisekharan R. Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events. Nat Biotechnol. 2008 Jun; 26(6):669-75.
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Srinivasan A, Viswanathan K, Raman R, Chandrasekaran A, Raguram S, Tumpey TM, Sasisekharan V, Sasisekharan R. Quantitative biochemical rationale for differences in transmissibility of 1918 pandemic influenza A viruses. Proc Natl Acad Sci U S A. 2008 Feb 26; 105(8):2800-5.
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Chandrasekaran A, Srinivasan A, Raman R, Viswanathan K, Raguram S, Tumpey TM, Sasisekharan V, Sasisekharan R. Glycan topology determines human adaptation of avian H5N1 virus hemagglutinin. Nat Biotechnol. 2008 Jan; 26(1):107-13.
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Packer NH, von der Lieth CW, Aoki-Kinoshita KF, Lebrilla CB, Paulson JC, Raman R, Rudd P, Sasisekharan R, Taniguchi N, York WS. Frontiers in glycomics: bioinformatics and biomarkers in disease. An NIH white paper prepared from discussions by the focus groups at a workshop on the NIH campus, Bethesda MD (September 11-13, 2006). Proteomics. 2008 Jan; 8(1):8-20.
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Raman R, Sasisekharan R. Cooperativity in glycan-protein interactions. Chem Biol. 2007 Aug; 14(8):873-4.
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Guerrini M, Guglieri S, Naggi A, Sasisekharan R, Torri G. Low molecular weight heparins: structural differentiation by bidimensional nuclear magnetic resonance spectroscopy. Semin Thromb Hemost. 2007 Jul; 33(5):478-87.
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Yang Y, MacLeod V, Dai Y, Khotskaya-Sample Y, Shriver Z, Venkataraman G, Sasisekharan R, Naggi A, Torri G, Casu B, Vlodavsky I, Suva LJ, Epstein J, Yaccoby S, Shaughnessy JD, Barlogie B, Sanderson RD. The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy. Blood. 2007 Sep 15; 110(6):2041-8.
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Sengupta S, Sasisekharan R. Exploiting nanotechnology to target cancer. Br J Cancer. 2007 May 7; 96(9):1315-9.
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Vlodavsky I, Ilan N, Nadir Y, Brenner B, Katz BZ, Naggi A, Torri G, Casu B, Sasisekharan R. Heparanase, heparin and the coagulation system in cancer progression. Thromb Res. 2007; 120 Suppl 2:S112-20.
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Prabhakar V, Capila I, Raman R, Srinivasan A, Bosques CJ, Pojasek K, Wrick MA, Sasisekharan R. The catalytic machinery of chondroitinase ABC I utilizes a calcium coordination strategy to optimally process dermatan sulfate. Biochemistry. 2006 Sep 19; 45(37):11130-9.
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Bosques CJ, Raguram S, Sasisekharan R. The sweet side of biomarker discovery. Nat Biotechnol. 2006 Sep; 24(9):1100-1.
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Milovic NM, Behr JR, Godin M, Hou CS, Payer KR, Chandrasekaran A, Russo PR, Sasisekharan R, Manalis SR. Monitoring of heparin and its low-molecular-weight analogs by silicon field effect. Proc Natl Acad Sci U S A. 2006 Sep 5; 103(36):13374-9.
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Berry D, Lynn DM, Berry E, Sasisekharan R, Langer R. Heparin localization and fine structure regulate Burkitt's lymphoma growth. Biochem Biophys Res Commun. 2006 Sep 29; 348(3):850-6.
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Shaya D, Tocilj A, Li Y, Myette J, Venkataraman G, Sasisekharan R, Cygler M. Crystal structure of heparinase II from Pedobacter heparinus and its complex with a disaccharide product. J Biol Chem. 2006 Jun 2; 281(22):15525-35.
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Sasisekharan R. Ram Sasisekharan. Interview. Lancet. 2006 Mar 11; 367(9513):809.
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Raman R, Venkataraman M, Ramakrishnan S, Lang W, Raguram S, Sasisekharan R. Advancing glycomics: implementation strategies at the consortium for functional glycomics. Glycobiology. 2006 May; 16(5):82R-90R.
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Prabhakar V, Sasisekharan R. The biosynthesis and catabolism of galactosaminoglycans. Adv Pharmacol. 2006; 53:69-115.
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Vlodavsky I, Abboud-Jarrous G, Elkin M, Naggi A, Casu B, Sasisekharan R, Ilan N. The impact of heparanese and heparin on cancer metastasis and angiogenesis. Pathophysiol Haemost Thromb. 2006; 35(1-2):116-27.
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Sasisekharan R, Raman R, Prabhakar V. Glycomics approach to structure-function relationships of glycosaminoglycans. Annu Rev Biomed Eng. 2006; 8:181-231.
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Raman R, Raguram S, Venkataraman G, Paulson JC, Sasisekharan R. Glycomics: an integrated systems approach to structure-function relationships of glycans. Nat Methods. 2005 Nov; 2(11):817-24.
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Dai Y, Yang Y, MacLeod V, Yue X, Rapraeger AC, Shriver Z, Venkataraman G, Sasisekharan R, Sanderson RD. HSulf-1 and HSulf-2 are potent inhibitors of myeloma tumor growth in vivo. J Biol Chem. 2005 Dec 2; 280(48):40066-73.
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Prabhakar V, Raman R, Capila I, Bosques CJ, Pojasek K, Sasisekharan R. Biochemical characterization of the chondroitinase ABC I active site. Biochem J. 2005 Sep 1; 390(Pt 2):395-405.
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Sengupta S, Eavarone D, Capila I, Zhao G, Watson N, Kiziltepe T, Sasisekharan R. Temporal targeting of tumour cells and neovasculature with a nanoscale delivery system. Nature. 2005 Jul 28; 436(7050):568-72.
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Sengupta S, Sellers LA, Gherardi E, Sasisekharan R, Fan TP. Nitric oxide modulates hepatocyte growth factor/scatter factor-induced angiogenesis. Angiogenesis. 2004; 7(4):285-94.
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Iyengar P, Espina V, Williams TW, Lin Y, Berry D, Jelicks LA, Lee H, Temple K, Graves R, Pollard J, Chopra N, Russell RG, Sasisekharan R, Trock BJ, Lippman M, Calvert VS, Petricoin EF, Liotta L, Dadachova E, Pestell RG, Lisanti MP, Bonaldo P, Scherer PE. Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment. J Clin Invest. 2005 May; 115(5):1163-76.
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Raman R, Sasisekharan V, Sasisekharan R. Structural insights into biological roles of protein-glycosaminoglycan interactions. Chem Biol. 2005 Mar; 12(3):267-77.
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Prabhakar V, Capila I, Bosques CJ, Pojasek K, Sasisekharan R. Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant expression and active site identification. Biochem J. 2005 Feb 15; 386(Pt 1):103-12.
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Behr JR, Matsumoto Y, White FM, Sasisekharan R. Quantification of isomers from a mixture of twelve heparin and heparan sulfate disaccharides using tandem mass spectrometry. Rapid Commun Mass Spectrom. 2005; 19(18):2553-62.
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Berry D, Ren J, Kwan CP, Sietsma DK, Sasisekharan R, Finklestein SP. Dimeric fibroblast growth factor-2 enhances functional recovery after focal cerebral ischemia. Restor Neurol Neurosci. 2005; 23(3-4):251-6.
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Shriver Z, Raguram S, Sasisekharan R. Glycomics: a pathway to a class of new and improved therapeutics. Nat Rev Drug Discov. 2004 Oct; 3(10):863-73.
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Sengupta S, Toh SA, Sellers LA, Skepper JN, Koolwijk P, Leung HW, Yeung HW, Wong RN, Sasisekharan R, Fan TP. Modulating angiogenesis: the yin and the yang in ginseng. Circulation. 2004 Sep 7; 110(10):1219-25.
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Sengupta S, Kiziltepe T, Sasisekharan R. A dual-color fluorescence imaging-based system for the dissection of antiangiogenic and chemotherapeutic activity of molecules. FASEB J. 2004 Oct; 18(13):1565-7.
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Qi Y, Zhao G, Liu D, Shriver Z, Sundaram M, Sengupta S, Venkataraman G, Langer R, Sasisekharan R. Delivery of therapeutic levels of heparin and low-molecular-weight heparin through a pulmonary route. Proc Natl Acad Sci U S A. 2004 Jun 29; 101(26):9867-72.
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Michel G, Pojasek K, Li Y, Sulea T, Linhardt RJ, Raman R, Prabhakar V, Sasisekharan R, Cygler M. The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic machinery. J Biol Chem. 2004 Jul 30; 279(31):32882-96.
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Berry D, Lynn DM, Sasisekharan R, Langer R. Poly(beta-amino ester)s promote cellular uptake of heparin and cancer cell death. Chem Biol. 2004 Apr; 11(4):487-98.
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Berry D, Shriver Z, Venkataraman G, Sasisekharan R. Quantitative assessment of FGF regulation by cell surface heparan sulfates. Biochem Biophys Res Commun. 2004 Feb 20; 314(4):994-1000.
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Goldoni S, Owens RT, McQuillan DJ, Shriver Z, Sasisekharan R, Birk DE, Campbell S, Iozzo RV. Biologically active decorin is a monomer in solution. J Biol Chem. 2004 Feb 20; 279(8):6606-12.
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Sengupta S, Sellers LA, Cindrova T, Skepper J, Gherardi E, Sasisekharan R, Fan TP. Cyclooxygenase-2-selective nonsteroidal anti-inflammatory drugs inhibit hepatocyte growth factor/scatter factor-induced angiogenesis. Cancer Res. 2003 Dec 1; 63(23):8351-9.
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Johnson NA, Sengupta S, Saidi SA, Lessan K, Charnock-Jones SD, Scott L, Stephens R, Freeman TC, Tom BD, Harris M, Denyer G, Sundaram M, Sasisekharan R, Smith SK, Print CG. Endothelial cells preparing to die by apoptosis initiate a program of transcriptome and glycome regulation. FASEB J. 2004 Jan; 18(1):188-90.
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Berry D, Shriver Z, Natke B, Kwan CP, Venkataraman G, Sasisekharan R. Heparan sulphate glycosaminoglycans derived from endothelial cells and smooth muscle cells differentially modulate fibroblast growth factor-2 biological activity through fibroblast growth factor receptor-1. Biochem J. 2003 Jul 1; 373(Pt 1):241-9.
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Sengupta S, Sellers LA, Li RC, Gherardi E, Zhao G, Watson N, Sasisekharan R, Fan TP. Targeting of mitogen-activated protein kinases and phosphatidylinositol 3 kinase inhibits hepatocyte growth factor/scatter factor-induced angiogenesis. Circulation. 2003 Jun 17; 107(23):2955-61.
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Dull RO, Dinavahi R, Schwartz L, Humphries DE, Berry D, Sasisekharan R, Garcia JG. Lung endothelial heparan sulfates mediate cationic peptide-induced barrier dysfunction: a new role for the glycocalyx. Am J Physiol Lung Cell Mol Physiol. 2003 Nov; 285(5):L986-95.
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Raman R, Venkataraman G, Ernst S, Sasisekharan V, Sasisekharan R. Structural specificity of heparin binding in the fibroblast growth factor family of proteins. Proc Natl Acad Sci U S A. 2003 Mar 4; 100(5):2357-62.
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Sundaram M, Qi Y, Shriver Z, Liu D, Zhao G, Venkataraman G, Langer R, Sasisekharan R. Rational design of low-molecular weight heparins with improved in vivo activity. Proc Natl Acad Sci U S A. 2003 Jan 21; 100(2):651-6.
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Raman R, Myette JR, Shriver Z, Pojasek K, Venkataraman G, Sasisekharan R. The heparin/heparan sulfate 2-O-sulfatase from Flavobacterium heparinum. A structural and biochemical study of the enzyme active site and saccharide substrate specificity. J Biol Chem. 2003 Apr 4; 278(14):12167-74.
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Myette JR, Shriver Z, Claycamp C, McLean MW, Venkataraman G, Sasisekharan R. The heparin/heparan sulfate 2-O-sulfatase from Flavobacterium heparinum. Molecular cloning, recombinant expression, and biochemical characterization. J Biol Chem. 2003 Apr 4; 278(14):12157-66.
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Sengupta S, Gherardi E, Sellers LA, Wood JM, Sasisekharan R, Fan TP. Hepatocyte growth factor/scatter factor can induce angiogenesis independently of vascular endothelial growth factor. Arterioscler Thromb Vasc Biol. 2003 Jan 1; 23(1):69-75.
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Guerrini M, Raman R, Venkataraman G, Torri G, Sasisekharan R, Casu B. A novel computational approach to integrate NMR spectroscopy and capillary electrophoresis for structure assignment of heparin and heparan sulfate oligosaccharides. Glycobiology. 2002 Nov; 12(11):713-9.
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Sasisekharan R, Shriver Z, Venkataraman G, Narayanasami U. Roles of heparan-sulphate glycosaminoglycans in cancer. Nat Rev Cancer. 2002 Jul; 2(7):521-8.
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Liu J, Shriver Z, Pope RM, Thorp SC, Duncan MB, Copeland RJ, Raska CS, Yoshida K, Eisenberg RJ, Cohen G, Linhardt RJ, Sasisekharan R. Characterization of a heparan sulfate octasaccharide that binds to herpes simplex virus type 1 glycoprotein D. J Biol Chem. 2002 Sep 6; 277(36):33456-67.
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Pojasek K, Raman R, Kiley P, Venkataraman G, Sasisekharan R. Biochemical characterization of the chondroitinase B active site. J Biol Chem. 2002 Aug 23; 277(34):31179-86.
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Myette JR, Shriver Z, Kiziltepe T, McLean MW, Venkataraman G, Sasisekharan R. Molecular cloning of the heparin/heparan sulfate delta 4,5 unsaturated glycuronidase from Flavobacterium heparinum, its recombinant expression in Escherichia coli, and biochemical determination of its unique substrate specificity. Biochemistry. 2002 Jun 11; 41(23):7424-34.
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Raman R, Myette J, Venkataraman G, Sasisekharan V, Sasisekharan R. Identification of structural motifs and amino acids within the structure of human heparan sulfate 3-O-sulfotransferase that mediate enzymatic function. Biochem Biophys Res Commun. 2002 Feb 1; 290(4):1214-9.
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Liu D, Shriver Z, Qi Y, Venkataraman G, Sasisekharan R. Dynamic regulation of tumor growth and metastasis by heparan sulfate glycosaminoglycans. Semin Thromb Hemost. 2002 Feb; 28(1):67-78.
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Shriver Z, Liu D, Sasisekharan R. Emerging views of heparan sulfate glycosaminoglycan structure/activity relationships modulating dynamic biological functions. Trends Cardiovasc Med. 2002 Feb; 12(2):71-7.
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Myette JR, Shriver Z, Liu J, Venkataraman G, Rosenberg R, Sasisekharan R. Expression in Escherichia coli, purification and kinetic characterization of human heparan sulfate 3-O-sulfotransferase-1. Biochem Biophys Res Commun. 2002 Feb 1; 290(4):1206-13.
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Liu D, Shriver Z, Venkataraman G, El Shabrawi Y, Sasisekharan R. Tumor cell surface heparan sulfate as cryptic promoters or inhibitors of tumor growth and metastasis. Proc Natl Acad Sci U S A. 2002 Jan 22; 99(2):568-73.
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Zhang L, Beeler DL, Lawrence R, Lech M, Liu J, Davis JC, Shriver Z, Sasisekharan R, Rosenberg RD. 6-O-sulfotransferase-1 represents a critical enzyme in the anticoagulant heparan sulfate biosynthetic pathway. J Biol Chem. 2001 Nov 9; 276(45):42311-21.
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Pojasek K, Shriver Z, Kiley P, Venkataraman G, Sasisekharan R. Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum. Biochem Biophys Res Commun. 2001 Aug 17; 286(2):343-51.
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Berry D, Kwan CP, Shriver Z, Venkataraman G, Sasisekharan R. Distinct heparan sulfate glycosaminoglycans are responsible for mediating fibroblast growth factor-2 biological activity through different fibroblast growth factor receptors. FASEB J. 2001 Jun; 15(8):1422-4.
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Kwan CP, Venkataraman G, Shriver Z, Raman R, Liu D, Qi Y, Varticovski L, Sasisekharan R. Probing fibroblast growth factor dimerization and role of heparin-like glycosaminoglycans in modulating dimerization and signaling. J Biol Chem. 2001 Jun 29; 276(26):23421-9.
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Karumanchi SA, Jha V, Ramchandran R, Karihaloo A, Tsiokas L, Chan B, Dhanabal M, Hanai JI, Venkataraman G, Shriver Z, Keiser N, Kalluri R, Zeng H, Mukhopadhyay D, Chen RL, Lander AD, Hagihara K, Yamaguchi Y, Sasisekharan R, Cantley L, Sukhatme VP. Cell surface glypicans are low-affinity endostatin receptors. Mol Cell. 2001 Apr; 7(4):811-22.
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Keiser N, Venkataraman G, Shriver Z, Sasisekharan R. Direct isolation and sequencing of specific protein-binding glycosaminoglycans. Nat Med. 2001 Jan; 7(1):123-8.
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Sasisekharan R, Venkataraman G. Heparin and heparan sulfate: biosynthesis, structure and function. Curr Opin Chem Biol. 2000 Dec; 4(6):626-31.
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Plotnikov AN, Eliseenkova AV, Ibrahimi OA, Shriver Z, Sasisekharan R, Lemmon MA, Mohammadi M. Crystal structure of fibroblast growth factor 9 reveals regions implicated in dimerization and autoinhibition. J Biol Chem. 2001 Feb 9; 276(6):4322-9.
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Shriver Z, Raman R, Venkataraman G, Drummond K, Turnbull J, Toida T, Linhardt R, Biemann K, Sasisekharan R. Sequencing of 3-O sulfate containing heparin decasaccharides with a partial antithrombin III binding site. Proc Natl Acad Sci U S A. 2000 Sep 12; 97(19):10359-64.
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Shriver Z, Sundaram M, Venkataraman G, Fareed J, Linhardt R, Biemann K, Sasisekharan R. Cleavage of the antithrombin III binding site in heparin by heparinases and its implication in the generation of low molecular weight heparin. Proc Natl Acad Sci U S A. 2000 Sep 12; 97(19):10365-70.
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Pojasek K, Shriver Z, Hu Y, Sasisekharan R. Histidine 295 and histidine 510 are crucial for the enzymatic degradation of heparan sulfate by heparinase III. Biochemistry. 2000 Apr 11; 39(14):4012-9.
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Liu J, Shriver Z, Blaiklock P, Yoshida K, Sasisekharan R, Rosenberg RD. Heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A sulfates N-unsubstituted glucosamine residues. J Biol Chem. 1999 Dec 31; 274(53):38155-62.
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Venkataraman G, Shriver Z, Raman R, Sasisekharan R. Sequencing complex polysaccharides. Science. 1999 Oct 15; 286(5439):537-42.
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Padera R, Venkataraman G, Berry D, Godavarti R, Sasisekharan R. FGF-2/fibroblast growth factor receptor/heparin-like glycosaminoglycan interactions: a compensation model for FGF-2 signaling. FASEB J. 1999 Oct; 13(13):1677-87.
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Davis JC, Venkataraman G, Shriver Z, Raj PA, Sasisekharan R. Oligomeric self-association of basic fibroblast growth factor in the absence of heparin-like glycosaminoglycans. Biochem J. 1999 Aug 1; 341 ( Pt 3):613-20.
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Ameer GA, Raghavan S, Sasisekharan R, Harmon W, Cooney CL, Langer R. Regional heparinization via simultaneous separation and reaction in a novel Taylor-Couette flow device. Biotechnol Bioeng. 1999 Jun 5; 63(5):618-24.
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Venkataraman G, Raman R, Sasisekharan V, Sasisekharan R. Molecular characteristics of fibroblast growth factor-fibroblast growth factor receptor-heparin-like glycosaminoglycan complex. Proc Natl Acad Sci U S A. 1999 Mar 30; 96(7):3658-63.
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Ameer GA, Harmon W, Sasisekharan R, Langer R. Investigation of a whole blood fluidized bed Taylor-Couette flow device for enzymatic heparin neutralization. Biotechnol Bioeng. 1999 Mar 5; 62(5):602-8.
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Ameer GA, Barabino G, Sasisekharan R, Harmon W, Cooney CL, Langer R. Ex vivo evaluation of a Taylor-Couette flow, immobilized heparinase I device for clinical application. Proc Natl Acad Sci U S A. 1999 Mar 2; 96(5):2350-5.
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Venkataraman G, Shriver Z, Davis JC, Sasisekharan R. Fibroblast growth factors 1 and 2 are distinct in oligomerization in the presence of heparin-like glycosaminoglycans. Proc Natl Acad Sci U S A. 1999 Mar 2; 96(5):1892-7.
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Shriver Z, Liu D, Hu Y, Sasisekharan R. Biochemical investigations and mapping of the calcium-binding sites of heparinase I from Flavobacterium heparinum. J Biol Chem. 1999 Feb 12; 274(7):4082-8.
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Liu D, Shriver Z, Godavarti R, Venkataraman G, Sasisekharan R. The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymatic activity. J Biol Chem. 1999 Feb 12; 274(7):4089-95.
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Natke B, Venkataraman G, Nugent MA, Sasisekharan R. Heparinase treatment of bovine smooth muscle cells inhibits fibroblast growth factor-2 binding to fibroblast growth factor receptor but not FGF-2 mediated cellular proliferation. Angiogenesis. 1999; 3(3):249-57.
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Rhomberg AJ, Shriver Z, Biemann K, Sasisekharan R. Mass spectrometric evidence for the enzymatic mechanism of the depolymerization of heparin-like glycosaminoglycans by heparinase II. Proc Natl Acad Sci U S A. 1998 Oct 13; 95(21):12232-7.
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Shriver Z, Hu Y, Pojasek K, Sasisekharan R. Heparinase II from Flavobacterium heparinum. Role of cysteine in enzymatic activity as probed by chemical modification and site- directed mutagenesis. J Biol Chem. 1998 Sep 4; 273(36):22904-12.
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Shriver Z, Hu Y, Sasisekharan R. Heparinase II from Flavobacterium heparinum. Role of histidine residues in enzymatic activity as probed by chemical modification and site-directed mutagenesis. J Biol Chem. 1998 Apr 24; 273(17):10160-7.
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Ernst S, Rhomberg AJ, Biemann K, Sasisekharan R. Direct evidence for a predominantly exolytic processive mechanism for depolymerization of heparin-like glycosaminoglycans by heparinase I. Proc Natl Acad Sci U S A. 1998 Apr 14; 95(8):4182-7.
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Rhomberg AJ, Ernst S, Sasisekharan R, Biemann K. Mass spectrometric and capillary electrophoretic investigation of the enzymatic degradation of heparin-like glycosaminoglycans. Proc Natl Acad Sci U S A. 1998 Apr 14; 95(8):4176-81.
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Godavarti R, Sasisekharan R. Heparinase I from Flavobacterium heparinum. Role of positive charge in enzymatic activity. J Biol Chem. 1998 Jan 2; 273(1):248-55.
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Armstrong D, Ueda T, Ueda T, Aljada A, Browne R, Fukuda S, Spengler R, Chou R, Hartnett M, Buch P, Dandona P, Sasisekharan R, Dorey CK. Lipid hydroperoxide stimulates retinal neovascularization in rabbit retina through expression of tumor necrosis factor-alpha, vascular endothelial growth factor and platelet-derived growth factor. Angiogenesis. 1998; 2(1):93-104.
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Herr AB, Ornitz DM, Sasisekharan R, Venkataraman G, Waksman G. Heparin-induced self-association of fibroblast growth factor-2. Evidence for two oligomerization processes. J Biol Chem. 1997 Jun 27; 272(26):16382-9.
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Ernst S, Garro OA, Winkler S, Venkataraman G, Langer R, Cooney CL, Sasisekharan R. Process simulation for recombinant protein production: cost estimation and sensitivity analysis for heparinase I expressed in Escherichia coli. Biotechnol Bioeng. 1997 Mar 20; 53(6):575-82.
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Godavarti R, Sasisekharan R. A comparative analysis of the primary sequences and characteristics of heparinases I, II, and III from Flavobacterium heparinum. Biochem Biophys Res Commun. 1996 Dec 24; 229(3):770-7.
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Godavarti R, Davis M, Venkataraman G, Cooney C, Langer R, Sasisekharan R. Heparinase III from Flavobacterium heparinum: cloning and recombinant expression in Escherichia coli. Biochem Biophys Res Commun. 1996 Aug 23; 225(3):751-8.
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Godavarti R, Cooney CL, Langer R, Sasisekharan R. Heparinase I from Flavobacterium heparinum. Identification of a critical histidine residue essential for catalysis as probed by chemical modification and site-directed mutagenesis. Biochemistry. 1996 May 28; 35(21):6846-52.
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Ernst S, Venkataraman G, Winkler S, Godavarti R, Langer R, Cooney CL, Sasisekharan R. Expression in Escherichia coli, purification and characterization of heparinase I from Flavobacterium heparinum. Biochem J. 1996 Apr 15; 315 ( Pt 2):589-97.
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Sasisekharan R, Venkataraman G, Godavarti R, Ernst S, Cooney CL, Langer R. Heparinase I from Flavobacterium heparinum. Mapping and characterization of the heparin binding domain. J Biol Chem. 1996 Feb 9; 271(6):3124-31.
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Venkataraman G, Sasisekharan V, Herr AB, Ornitz DM, Waksman G, Cooney CL, Langer R, Sasisekharan R. Preferential self-association of basic fibroblast growth factor is stabilized by heparin during receptor dimerization and activation. Proc Natl Acad Sci U S A. 1996 Jan 23; 93(2):845-50.
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Sasisekharan R, Leckband D, Godavarti R, Venkataraman G, Cooney CL, Langer R. Heparinase I from Flavobacterium heparinum: the role of the cysteine residue in catalysis as probed by chemical modification and site-directed mutagenesis. Biochemistry. 1995 Nov 7; 34(44):14441-8.
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Venkataraman G, Sasisekharan V, Cooney CL, Langer R, Sasisekharan R. Complex flexibility of the transforming growth factor beta superfamily. Proc Natl Acad Sci U S A. 1995 Jun 6; 92(12):5406-10.
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Ernst S, Langer R, Cooney CL, Sasisekharan R. Enzymatic degradation of glycosaminoglycans. Crit Rev Biochem Mol Biol. 1995; 30(5):387-444.
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Ferreira JP, Sasisekharan R, Louie O, Langer R. A study on the functional subunits of phospholipases A2 by enzyme immobilization. Biochem J. 1994 Oct 15; 303 ( Pt 2):527-30.
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Ferreira JP, Sasisekharan R, Louie O, Langer R. Carbodiimide modification enhances activity of pig pancreatic phospholipase A2. Eur J Biochem. 1994 Jul 15; 223(2):611-6.
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Venkataraman G, Sasisekharan V, Cooney CL, Langer R, Sasisekharan R. A stereochemical approach to pyranose ring flexibility: its implications for the conformation of dermatan sulfate. Proc Natl Acad Sci U S A. 1994 Jun 21; 91(13):6171-5.
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Sasisekharan R, Moses MA, Nugent MA, Cooney CL, Langer R. Heparinase inhibits neovascularization. Proc Natl Acad Sci U S A. 1994 Feb 15; 91(4):1524-8.
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Ferreira JP, Sasisekharan R, Louie O, Langer R. Influence of chemistry in immobilization of cobra venom phospholipase A2: implications as to mechanism. Biochemistry. 1993 Aug 17; 32(32):8098-102.
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Sasisekharan R, Bulmer M, Moremen KW, Cooney CL, Langer R. Cloning and expression of heparinase I gene from Flavobacterium heparinum. Proc Natl Acad Sci U S A. 1993 Apr 15; 90(8):3660-4.
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