Contact, publication, and social network information about Harvard faculty and fellows. Harvard Catalyst Profiles
Keywords
Last Name
Institution

Gerassimos M. Makrigiorgos, Ph.D.

TitleProfessor of Radiation Oncology
InstitutionBrigham and Women's Hospital
DepartmentRadiation Oncology
AddressBrigham and Women's Hospital
Radiation Oncology, L2
75 Francis St
Boston MA 02115
Phone617/525-7122
Fax617/582-6037

 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Tada DB, Singh S, Nagesha D, Jost E, Levy CO, Gultepe E, Cormack R, Makrigiorgos GM, Sridhar S. Chitosan film containing poly(D,L-lactic-co-glycolic acid) nanoparticles: a platform for localized dual-drug release. Pharm Res. 2010 Aug; 27(8):1738-45.
    View in: PubMed
  2. Zhu X, Palmer MR, Makrigiorgos GM, Kassis AI. Solid-tumor radionuclide therapy dosimetry: new paradigms in view of tumor microenvironment and angiogenesis. Med Phys. 2010 Jun; 37(6):2974-84.
    View in: PubMed
  3. Li J, Milbury CA, Li C, Makrigiorgos GM. Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma. Hum Mutat. 2009 Nov; 30(11):1583-90.
    View in: PubMed
  4. Cormack RA, Sridhar S, Suh WW, D'Amico AV, Makrigiorgos GM. Biological in situ dose painting for image-guided radiation therapy using drug-loaded implantable devices. Int J Radiat Oncol Biol Phys. 2010 Feb 1; 76(2):615-23.
    View in: PubMed
  5. Milbury CA, Li J, Makrigiorgos GM. COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutations. Clin Chem. 2009 Dec; 55(12):2130-43.
    View in: PubMed
  6. Lang JE, Magbanua MJ, Scott JH, Makrigiorgos GM, Wang G, Federman S, Esserman LJ, Park JW, Haqq CM. A comparison of RNA amplification techniques at sub-nanogram input concentration. BMC Genomics. 2009; 10:326.
    View in: PubMed
  7. Li J, Makrigiorgos GM. COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing. Biochem Soc Trans. 2009 Apr; 37(Pt 2):427-32.
    View in: PubMed
  8. Li J, Wang L, Jänne PA, Makrigiorgos GM. Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR. Clin Chem. 2009 Apr; 55(4):748-56.
    View in: PubMed
  9. Milbury CA, Li J, Makrigiorgos GM. PCR-based methods for the enrichment of minority alleles and mutations. Clin Chem. 2009 Apr; 55(4):632-40.
    View in: PubMed
  10. Chin DW, Treister N, Friedland B, Cormack RA, Tishler RB, Makrigiorgos GM, Court LE. Effect of dental restorations and prostheses on radiotherapy dose distribution: a Monte Carlo study. J Appl Clin Med Phys. 2009; 10(1):2853.
    View in: PubMed
  11. Mamon H, Hader C, Li J, Wang L, Kulke M, Amicarelli G, Shehi E, Adlerstein D, Roper K, Killion L, Hooshmand S, Makrigiorgos GM. Preferential amplification of apoptotic DNA from plasma: potential for enhancing detection of minor DNA alterations in circulating DNA. Clin Chem. 2008 Sep; 54(9):1582-4.
    View in: PubMed
  12. Li J, Wang L, Mamon H, Kulke MH, Berbeco R, Makrigiorgos GM. Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing. Nat Med. 2008 May; 14(5):579-84.
    View in: PubMed
  13. Park MA, Mahmood A, Zimmerman RE, Limpa-Amara N, Makrigiorgos GM, Moore SC. Adsorption of metallic radionuclides on plastic phantom walls. Med Phys. 2008 Apr; 35(4):1606-10.
    View in: PubMed
  14. Bonanno C, Shehi E, Adlerstein D, Makrigiorgos GM. MS-FLAG, a novel real-time signal generation method for methylation-specific PCR. Clin Chem. 2007 Dec; 53(12):2119-27.
    View in: PubMed
  15. Amicarelli G, Shehi E, Makrigiorgos GM, Adlerstein D. FLAG assay as a novel method for real-time signal generation during PCR: application to detection and genotyping of KRAS codon 12 mutations. Nucleic Acids Res. 2007; 35(19):e131.
    View in: PubMed
  16. Wang F, Wang L, Briggs C, Sicinska E, Gaston SM, Mamon H, Kulke MH, Zamponi R, Loda M, Maher E, Ogino S, Fuchs CS, Li J, Hader C, Makrigiorgos GM. DNA degradation test predicts success in whole-genome amplification from diverse clinical samples. J Mol Diagn. 2007 Sep; 9(4):441-51.
    View in: PubMed
  17. Li J, Berbeco R, Distel RJ, Jänne PA, Wang L, Makrigiorgos GM. s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis. Nucleic Acids Res. 2007; 35(12):e84.
    View in: PubMed
  18. Xiang HF, Song JS, Chin DW, Cormack RA, Tishler RB, Makrigiorgos GM, Court LE, Chin LM. Build-up and surface dose measurements on phantoms using micro-MOSFET in 6 and 10 MV x-ray beams and comparisons with Monte Carlo calculations. Med Phys. 2007 Apr; 34(4):1266-73.
    View in: PubMed
  19. Li J, Makrigiorgos GM. Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping. Nat Protoc. 2007; 2(1):50-8.
    View in: PubMed
  20. Amicarelli G, Adlerstein D, Shehi E, Wang F, Makrigiorgos GM. Genotype-specific signal generation based on digestion of 3-way DNA junctions: application to KRAS variation detection. Clin Chem. 2006 Oct; 52(10):1855-63.
    View in: PubMed
  21. Ogino S, Kawasaki T, Brahmandam M, Cantor M, Kirkner GJ, Spiegelman D, Makrigiorgos GM, Weisenberger DJ, Laird PW, Loda M, Fuchs CS. Precision and performance characteristics of bisulfite conversion and real-time PCR (MethyLight) for quantitative DNA methylation analysis. J Mol Diagn. 2006 May; 8(2):209-17.
    View in: PubMed
  22. Roy K, Wang L, Makrigiorgos GM, Price BD. Methylation of the ATM promoter in glioma cells alters ionizing radiation sensitivity. Biochem Biophys Res Commun. 2006 Jun 9; 344(3):821-6.
    View in: PubMed
  23. Li J, Wang F, Mamon H, Kulke MH, Harris L, Maher E, Wang L, Makrigiorgos GM. Antiprimer quenching-based real-time PCR and its application to the analysis of clinical cancer samples. Clin Chem. 2006 Apr; 52(4):624-33.
    View in: PubMed
  24. Wang F, Kaur M, Liu WH, Zhu P, Li J, Wang L, Makrigiorgos GM. High-throughput scanning of breast tumor surgical specimens for low-level mutations. Clin Chem Lab Med. 2005; 43(8):810-6.
    View in: PubMed
  25. Wang G, Maher E, Brennan C, Chin L, Leo C, Kaur M, Zhu P, Rook M, Wolfe JL, Makrigiorgos GM. DNA amplification method tolerant to sample degradation. Genome Res. 2004 Nov; 14(11):2357-66.
    View in: PubMed
  26. Wang G, Brennan C, Rook M, Wolfe JL, Leo C, Chin L, Pan H, Liu WH, Price B, Makrigiorgos GM. Balanced-PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples. Nucleic Acids Res. 2004; 32(9):e76.
    View in: PubMed
  27. Chen S, Wang G, Makrigiorgos GM, Price BD. Stable siRNA-mediated silencing of ATM alters the transcriptional profile of HeLa cells. Biochem Biophys Res Commun. 2004 May 14; 317(4):1037-44.
    View in: PubMed
  28. Liu WH, Kaur M, Wang G, Zhu P, Zhang Y, Makrigiorgos GM. Inverse PCR-based RFLP scanning identifies low-level mutation signatures in colon cells and tumors. Cancer Res. 2004 Apr 1; 64(7):2544-51.
    View in: PubMed
  29. Liu WH, Makrigiorgos GM. Sensitive and quantitative detection of mutations associated with clinical resistance to STI-571. Leuk Res. 2003 Nov; 27(11):979-82.
    View in: PubMed
  30. Liu WH, Kaur M, Makrigiorgos GM. Detection of hotspot mutations and polymorphisms using an enhanced PCR-RFLP approach. Hum Mutat. 2003 May; 21(5):535-41.
    View in: PubMed
  31. Kaur M, Makrigiorgos GM. Novel amplification of DNA in a hairpin structure: towards a radical elimination of PCR errors from amplified DNA. Nucleic Acids Res. 2003 Mar 15; 31(6):e26.
    View in: PubMed
  32. Xue LY, Butler NJ, Makrigiorgos GM, Adelstein SJ, Kassis AI. Bystander effect produced by radiolabeled tumor cells in vivo. Proc Natl Acad Sci U S A. 2002 Oct 15; 99(21):13765-70.
    View in: PubMed
  33. Kaur M, Zhang Y, Liu WH, Tetradis S, Price BD, Makrigiorgos GM. Ligation of a primer at a mutation: a method to detect low level mutations in DNA. Mutagenesis. 2002 Sep; 17(5):365-74.
    View in: PubMed
  34. Makrigiorgos GM, Chakrabarti S, Zhang Y, Kaur M, Price BD. A PCR-based amplification method retaining the quantitative difference between two complex genomes. Nat Biotechnol. 2002 Sep; 20(9):936-9.
    View in: PubMed
  35. Zhang Y, Kaur M, Price BD, Tetradis S, Makrigiorgos GM. An amplification and ligation-based method to scan for unknown mutations in DNA. Hum Mutat. 2002 Aug; 20(2):139-47.
    View in: PubMed
  36. Maulik G, Salgia R, Makrigiorgos GM. Flow cytometric determination of lipid peroxidation using fluoresceinated phosphoethanolamine. Methods Enzymol. 2002; 352:80-91.
    View in: PubMed
  37. Zhang Y, Price BD, Tetradis S, Chakrabarti S, Maulik G, Makrigiorgos GM. Reproducible and inexpensive probe preparation for oligonucleotide arrays. Nucleic Acids Res. 2001 Jul 1; 29(13):E66-6.
    View in: PubMed
  38. Chakrabarti S, Price BD, Tetradis S, Fox EA, Zhang Y, Maulik G, Makrigiorgos GM. Highly selective isolation of unknown mutations in diverse DNA fragments: toward new multiplex screening in cancer. Cancer Res. 2000 Jul 15; 60(14):3732-7.
    View in: PubMed
  39. Chakrabarti S, Mahmood A, Makrigiorgos GM. Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III). Int J Radiat Biol. 1999 Aug; 75(8):1055-65.
    View in: PubMed
  40. Maulik G, Botchway S, Chakrabarti S, Tetradis S, Price B, Makrigiorgos GM. Novel non-isotopic detection of MutY enzyme-recognized mismatches in DNA via ultrasensitive detection of aldehydes. Nucleic Acids Res. 1999 Mar 1; 27(5):1316-22.
    View in: PubMed
  41. Makrigiorgos GM. Detection of chromatin-associated hydroxyl radicals generated by DNA-bound metal compounds and antitumor antibiotics. Met Ions Biol Syst. 1999; 36:521-45.
    View in: PubMed
  42. Maulik G, Kassis AI, Savvides P, Makrigiorgos GM. Fluoresceinated phosphoethanolamine for flow-cytometric measurement of lipid peroxidation. Free Radic Biol Med. 1998 Oct; 25(6):645-53.
    View in: PubMed
  43. Makrigiorgos GM, Chakrabarti S, Mahmood A. Fluorescent labelling of abasic sites: a novel methodology to detect closely-spaced damage sites in DNA. Int J Radiat Biol. 1998 Jul; 74(1):99-109.
    View in: PubMed
  44. Kassis AI, Wen PY, Van den Abbeele AD, Baranowska-Kortylewicz J, Makrigiorgos GM, Metz KR, Matalka KZ, Cook CU, Sahu SK, Black PM, Adelstein SJ. 5-[125I]iodo-2'-deoxyuridine in the radiotherapy of brain tumors in rats. J Nucl Med. 1998 Jul; 39(7):1148-54.
    View in: PubMed
  45. Botchway SW, Chakrabarti S, Makrigiorgos GM. Novel visible and ultraviolet light photogeneration of hydroxyl radicals by 2-methyl-4-nitro-quinoline-N-oxide (MNO) and 4,4'-dinitro-(2,2')bipyridinyl-N,N'-dioxide (DBD). Photochem Photobiol. 1998 Jun; 67(6):635-40.
    View in: PubMed
  46. Chakrabarti S, Kassis AI, Slayter HS, Bump EA, Sahu SK, Makrigiorgos GM. Continuous detection of radiation or metal generated hydroxyl radicals within core chromatin particles. Int J Radiat Biol. 1998 Jan; 73(1):53-63.
    View in: PubMed
  47. Makrigiorgos GM. Detection of lipid peroxidation on erythrocytes using the excimer-forming property of a lipophilic BODIPY fluorescent dye. J Biochem Biophys Methods. 1997 Aug 1; 35(1):23-35.
    View in: PubMed
  48. Makrigiorgos GM, Kassis AI, Mahmood A, Bump EA, Savvides P. Novel fluorescein-based flow-cytometric method for detection of lipid peroxidation. Free Radic Biol Med. 1997; 22(1-2):93-100.
    View in: PubMed
  49. Chakrabarti S, Mahmood A, Kassis AI, Bump EA, Jones AG, Makrigiorgos GM. Generation of hydroxyl radicals by nucleohistone-bound metal-adriamycin complexes. Free Radic Res. 1996 Sep; 25(3):207-20.
    View in: PubMed
  50. Chakrabarti S, Makrigiorgos GM, O'Brien K, Bump E, Kassis AI. Measurement of hydroxyl radicals catalyzed in the immediate vicinity of DNA by metal-bleomycin complexes. Free Radic Biol Med. 1996; 20(6):777-83.
    View in: PubMed
  51. Makrigiorgos GM, Bump E, Huang C, Baranowska-Kortylewicz J, Kassis AI. A fluorimetric method for the detection of copper-mediated hydroxyl free radicals in the immediate proximity of DNA. Free Radic Biol Med. 1995 Apr; 18(4):669-78.
    View in: PubMed
  52. Sahu SK, Kassis AI, Makrigiorgos GM, Baranowska-Kortylewicz J, Adelstein SJ. The effects of indium-111 decay on pBR322 DNA. Radiat Res. 1995 Feb; 141(2):193-8.
    View in: PubMed
  53. Collins AK, Makrigiorgos GM, Svensson GK. Coumarin chemical dosimeter for radiation therapy. Med Phys. 1994 Nov; 21(11):1741-7.
    View in: PubMed
  54. Makrigiorgos GM, Bump E, Huang C, Baranowska-Kortylewicz J, Kassis AI. Accessibility of nucleic acid-complexed biomolecules to hydroxyl radicals correlates with their conformation: a fluorescence polarization spectroscopy study. Int J Radiat Biol. 1994 Sep; 66(3):247-57.
    View in: PubMed
  55. Makrigiorgos GM, Folkard M, Huang C, Bump E, Baranowska-Kortylewicz J, Sahu SK, Michael BD, Kassis AI. Quantification of radiation-induced hydroxyl radicals within nucleohistones using a molecular fluorescent probe. Radiat Res. 1994 May; 138(2):177-85.
    View in: PubMed
  56. Makrigiorgos GM, Baranowska-Kortylewicz J, Bump E, Sahu SK, Berman RM, Kassis AI. A method for detection of hydroxyl radicals in the vicinity of biomolecules using radiation-induced fluorescence of coumarin. Int J Radiat Biol. 1993 Apr; 63(4):445-58.
    View in: PubMed
  57. Makrigiorgos GM, Berman RM, Baranowska-Kortylewicz J, Bump E, Humm JL, Adelstein SJ, Kassis AI. DNA damage produced in V79 cells by DNA-incorporated iodine-123: a comparison with iodine-125. Radiat Res. 1992 Mar; 129(3):309-14.
    View in: PubMed
  58. Baranowska-Kortylewicz J, Makrigiorgos GM, Van den Abbeele AD, Berman RM, Adelstein SJ, Kassis AI. 5-[123I]iodo-2'-deoxyuridine in the radiotherapy of an early ascites tumor model. Int J Radiat Oncol Biol Phys. 1991 Nov; 21(6):1541-51.
    View in: PubMed
  59. Makrigiorgos GM, Adelstein SJ, Kassis AI. Cellular radiation dosimetry and its implications for estimation of radiation risks. Illustrative results with technetium 99m-labeled microspheres and macroaggregates. JAMA. 1990 Aug 1; 264(5):592-5.
    View in: PubMed
  60. Makrigiorgos GM, Ito S, Baranowska-Kortylewicz J, Vinter DW, Iqbal A, Van den Abbeele AD, Adelstein SJ, Kassis AI. Inhomogeneous deposition of radiopharmaceuticals at the cellular level: experimental evidence and dosimetric implications. J Nucl Med. 1990 Aug; 31(8):1358-63.
    View in: PubMed
  61. Makrigiorgos GM, Smathers JB, Myers LT, Benedict SH. Radiation quality investigations around a neutron therapy beam, using a high-pressure ionization chamber. Med Phys. 1990 Jan-Feb; 17(1):84-6.
    View in: PubMed
  62. Makrigiorgos G, Adelstein SJ, Kassis AI. Auger electron emitters: insights gained from in vitro experiments. Radiat Environ Biophys. 1990; 29(2):75-91.
    View in: PubMed
  63. Makrigiorgos GM, Adelstein SJ, Kassis AI. Limitations of conventional internal dosimetry at the cellular level. J Nucl Med. 1989 Nov; 30(11):1856-64.
    View in: PubMed
  64. Makrigiorgos GM. Derivation of radiation quality average parameters in neutron-gamma radiation fields with the high-pressure ionization chamber: theory and practice. Radiat Res. 1989 Jun; 118(3):387-400.
    View in: PubMed
  65. Makrigiorgos GM, Kassis AI, Baranowska-Kortylewicz J, McElvany KD, Welch MJ, Sastry KS, Adelstein SJ. Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells: a comparison with 5-[125I]iodo-2'-deoxyuridine. Radiat Res. 1989 Jun; 118(3):532-44.
    View in: PubMed
  66. Folkard M, Makrigiorgos G, Roper MJ, Waker AJ, Michael BD. Measurements of neutron energy using a recoil-proton telescope and a high-pressure ionization chamber. Radiat Res. 1989 Apr; 118(1):37-45.
    View in: PubMed
  67. Makrigiorgos G, Antonadou D, Proukakis C, Throuvalas N. Measurement of the restricted linear energy transfer of stray radiation close to the treatment volume of 12 and 18 MeV clinical photon beams. Med Phys. 1989 Mar-Apr; 16(2):302-4.
    View in: PubMed
  68. Lymberis C, Makrigiorgos G, Sbonias E, Polizois E, Mortzos G, Bacas A, Fountos A. Radiocesium levels in human muscle samples in Greece one year after the Chernobyl accident. Int J Rad Appl Instrum A. 1988; 39(2):175-6.
    View in: PubMed
  69. Makrigiorgos G. Characteristics of an ethylene-polyethylene high-pressure ionization chamber and its potential for deriving radiation dose and quality information in neutron-gamma radiation fields. Med Phys. 1988 Jan-Feb; 15(1):36-9.
    View in: PubMed
  70. Makrigiorgos G, Antonadou D. Measurement of the restricted dose mean LET outside the primary beam of a 60Co radiotherapy unit. Radiat Res. 1987 Apr; 110(1):142-8.
    View in: PubMed
  71. Makrigiorgos G, Waker AJ. The measurement of absorbed dose and dose-equivalent levels for in vivo neutron activation analysis. Phys Med Biol. 1985 Sep; 30(9):909-19.
    View in: PubMed
Local representatives can answer questions about the Profiles website or help with editing a profile or issues with profile data. For assistance with this profile: HMS/HSDM faculty should contact Human Resources at faculty_serviceshms.harvard.edu.
Makrigiorgos's Networks
Click the "See All" links for more information and interactive visualizations!
Concepts
_
Co-Authors
_
Similar People
_
Same Department
Physical Neighbors
_