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Last Name
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John Joseph Mekalanos, Ph.D.

TitleAdele Lehman Professor of Microbiology and Molecular Genetics
InstitutionHarvard Medical School
DepartmentMicrobiology and Immunobiology
AddressHarvard Medical School
MBIB, Room1035A
77 Avenue Louis Pasteur
Boston MA 02115
Phone617/432-1935
Fax617/738-7664
Other Positions
TitleMember of the Affiliated Faculty of the Harvard-MIT Division of Health Sciences and Technology
InstitutionMassachusetts Institute of Technology
DepartmentHealth Sciences and Technology

TitleHead of the Department of Microbiology and Immunobiology
InstitutionHarvard Medical School
DepartmentMicrobiology and Immunobiology


 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Fu Y, Waldor MK, Mekalanos JJ. Tn-Seq Analysis of Vibrio cholerae Intestinal Colonization Reveals a Role for T6SS-Mediated Antibacterial Activity in the Host. Cell Host Microbe. 2013 Dec 11; 14(6):652-63.
    View in: PubMed
  2. Ho BT, Dong TG, Mekalanos JJ. A View to a Kill: The Bacterial Type VI Secretion System. Cell Host Microbe. 2014 Jan 15; 15(1):9-21.
    View in: PubMed
  3. Skurnik D, Roux D, Cattoir V, Danilchanka O, Lu X, Yoder-Himes DR, Han K, Guillard T, Jiang D, Gaultier C, Guerin F, Aschard H, Leclercq R, Mekalanos JJ, Lory S, Pier GB. Enhanced in vivo fitness of carbapenem-resistant oprD mutants of Pseudomonas aeruginosa revealed through high-throughput sequencing. Proc Natl Acad Sci U S A. 2013 Dec 17; 110(51):20747-52.
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  4. Ho BT, Basler M, Mekalanos JJ. Type 6 secretion system-mediated immunity to type 4 secretion system-mediated gene transfer. Science. 2013 Oct 11; 342(6155):250-3.
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  5. Mekalanos JJ. Vaccine economics: what price human life? Sci Transl Med. 2013 Sep 25; 5(204):204ed16.
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  6. Danilchanka O, Mekalanos JJ. Cyclic dinucleotides and the innate immune response. Cell. 2013 Aug 29; 154(5):962-70.
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  7. Shneider MM, Buth SA, Ho BT, Basler M, Mekalanos JJ, Leiman PG. PAAR-repeat proteins sharpen and diversify the type VI secretion system spike. Nature. 2013 Aug 15; 500(7462):350-3.
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  8. Bari SM, Roky MK, Mohiuddin M, Kamruzzaman M, Mekalanos JJ, Faruque SM. Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples. Proc Natl Acad Sci U S A. 2013 Jun 11; 110(24):9926-31.
    View in: PubMed
  9. Basler M, Ho BT, Mekalanos JJ. Tit-for-Tat: Type VI Secretion System Counterattack during Bacterial Cell-Cell Interactions. Cell. 2013 Feb 14; 152(4):884-94.
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  10. Robins WP, Faruque SM, Mekalanos JJ. Coupling mutagenesis and parallel deep sequencing to probe essential residues in a genome or gene. Proc Natl Acad Sci U S A. 2013 Feb 26; 110(9):E848-57.
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  11. Dong TG, Ho BT, Yoder-Himes DR, Mekalanos JJ. Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A. 2013 Feb 12; 110(7):2623-8.
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  12. Faruque SM, Mekalanos JJ. Phage-bacterial interactions in the evolution of toxigenic Vibrio cholerae. Virulence. 2012 Nov 15; 3(7):556-65.
    View in: PubMed
  13. Mekalanos JJ, Robins W, Ussery DW, Davis BM, Schadt E, Waldor MK. Non-O1 Vibrio cholerae unlinked to cholera in Haiti. Proc Natl Acad Sci U S A. 2012 Nov 20; 109(47):E3206.
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  14. Bogard RW, Davies BW, Mekalanos JJ. MetR-regulated Vibrio cholerae metabolism is required for virulence. MBio. 2012; 3(5).
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  15. Seed KD, Faruque SM, Mekalanos JJ, Calderwood SB, Qadri F, Camilli A. Phase variable O antigen biosynthetic genes control expression of the major protective antigen and bacteriophage receptor in Vibrio cholerae O1. PLoS Pathog. 2012 Sep; 8(9):e1002917.
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  16. Basler M, Mekalanos JJ. Type 6 secretion dynamics within and between bacterial cells. Science. 2012 Aug 17; 337(6096):815.
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  17. Bashir A, Klammer AA, Robins WP, Chin CS, Webster D, Paxinos E, Hsu D, Ashby M, Wang S, Peluso P, Sebra R, Sorenson J, Bullard J, Yen J, Valdovino M, Mollova E, Luong K, Lin S, LaMay B, Joshi A, Rowe L, Frace M, Tarr CL, Turnsek M, Davis BM, Kasarskis A, Mekalanos JJ, Waldor MK, Schadt EE. A hybrid approach for the automated finishing of bacterial genomes. Nat Biotechnol. 2012 Jul; 30(7):701-7.
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  18. Dong TG, Mekalanos JJ. Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52. Nucleic Acids Res. 2012 Sep; 40(16):7766-75.
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  19. Davies BW, Bogard RW, Young TS, Mekalanos JJ. Coordinated regulation of accessory genetic elements produces cyclic di-nucleotides for V. cholerae virulence. Cell. 2012 Apr 13; 149(2):358-70.
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  20. Basler M, Pilhofer M, Henderson GP, Jensen GJ, Mekalanos JJ. Type VI secretion requires a dynamic contractile phage tail-like structure. Nature. 2012 Mar 8; 483(7388):182-6.
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  21. Rattanama P, Thompson JR, Kongkerd N, Srinitiwarawong K, Vuddhakul V, Mekalanos JJ. Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic Vibrio harveyi. PLoS One. 2012; 7(2):e32523.
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  22. Zheng J, Ho B, Mekalanos JJ. Genetic analysis of anti-amoebae and anti-bacterial activities of the type VI secretion system in Vibrio cholerae. PLoS One. 2011; 6(8):e23876.
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  23. Mandlik A, Livny J, Robins WP, Ritchie JM, Mekalanos JJ, Waldor MK. RNA-Seq-based monitoring of infection-linked changes in Vibrio cholerae gene expression. Cell Host Microbe. 2011 Aug 18; 10(2):165-74.
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  24. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB. Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Front Microbiol. 2011; 2:162.
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  25. Davies BW, Bogard RW, Mekalanos JJ. Mapping the regulon of Vibrio cholerae ferric uptake regulator expands its known network of gene regulation. Proc Natl Acad Sci U S A. 2011 Jul 26; 108(30):12467-72.
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  26. Shin OS, Tam VC, Suzuki M, Ritchie JM, Bronson RT, Waldor MK, Mekalanos JJ. Type III secretion is essential for the rapidly fatal diarrheal disease caused by non-O1, non-O139 Vibrio cholerae. MBio. 2011; 2(3):e00106-11.
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  27. Farmer P, Almazor CP, Bahnsen ET, Barry D, Bazile J, Bloom BR, Bose N, Brewer T, Calderwood SB, Clemens JD, Cravioto A, Eustache E, Jérôme G, Gupta N, Harris JB, Hiatt HH, Holstein C, Hotez PJ, Ivers LC, Kerry VB, Koenig SP, Larocque RC, Léandre F, Lambert W, Lyon E, Mekalanos JJ, Mukherjee JS, Oswald C, Pape JW, Gretchko Prosper A, Rabinovich R, Raymonville M, Réjouit JR, Ronan LJ, Rosenberg ML, Ryan ET, Sachs JD, Sack DA, Surena C, Suri AA, Ternier R, Waldor MK, Walton D, Weigel JL. Meeting cholera's challenge to Haiti and the world: a joint statement on cholera prevention and care. PLoS Negl Trop Dis. 2011; 5(5):e1145.
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  28. Davies BW, Bogard RW, Dupes NM, Gerstenfeld TA, Simmons LA, Mekalanos JJ. DNA damage and reactive nitrogen species are barriers to Vibrio cholerae colonization of the infant mouse intestine. PLoS Pathog. 2011 Feb; 7(2):e1001295.
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  29. Zahid MS, Waise Z, Kamruzzaman M, Ghosh AN, Nair GB, Khairul Bashar SA, Mekalanos JJ, Faruque SM. An experimental study of phage mediated bactericidal selection & emergence of the El Tor Vibrio cholerae. Indian J Med Res. 2011 Feb; 133:218-24.
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  30. Chin CS, Sorenson J, Harris JB, Robins WP, Charles RC, Jean-Charles RR, Bullard J, Webster DR, Kasarskis A, Peluso P, Paxinos EE, Yamaichi Y, Calderwood SB, Mekalanos JJ, Schadt EE, Waldor MK. The origin of the Haitian cholera outbreak strain. N Engl J Med. 2011 Jan 6; 364(1):33-42.
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  31. Tam VC, Suzuki M, Coughlin M, Saslowsky D, Biswas K, Lencer WI, Faruque SM, Mekalanos JJ. Functional analysis of VopF activity required for colonization in Vibrio cholerae. MBio. 2010; 1(5).
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  32. Zheng J, Shin OS, Cameron DE, Mekalanos JJ. Quorum sensing and a global regulator TsrA control expression of type VI secretion and virulence in Vibrio cholerae. Proc Natl Acad Sci U S A. 2010 Dec 7; 107(49):21128-33.
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  33. Hassan F, Kamruzzaman M, Mekalanos JJ, Faruque SM. Satellite phage TLCf enables toxigenic conversion by CTX phage through dif site alteration. Nature. 2010 Oct 21; 467(7318):982-5.
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  34. Zahid MS, Waise TM, Kamruzzaman M, Ghosh AN, Nair GB, Mekalanos JJ, Faruque SM. The cyclic AMP (cAMP)-cAMP receptor protein signaling system mediates resistance of Vibrio cholerae O1 strains to multiple environmental bacteriophages. Appl Environ Microbiol. 2010 Jul; 76(13):4233-40.
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  35. Rui H, Ritchie JM, Bronson RT, Mekalanos JJ, Zhang Y, Waldor MK. Reactogenicity of live-attenuated Vibrio cholerae vaccines is dependent on flagellins. Proc Natl Acad Sci U S A. 2010 Mar 2; 107(9):4359-64.
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  36. Ma AT, Mekalanos JJ. In vivo actin cross-linking induced by Vibrio cholerae type VI secretion system is associated with intestinal inflammation. Proc Natl Acad Sci U S A. 2010 Mar 2; 107(9):4365-70.
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  37. Kamruzzaman M, Udden SM, Cameron DE, Calderwood SB, Nair GB, Mekalanos JJ, Faruque SM. Quorum-regulated biofilms enhance the development of conditionally viable, environmental Vibrio cholerae. Proc Natl Acad Sci U S A. 2010 Jan 26; 107(4):1588-93.
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  38. Flier JS, Mekalanos JJ. Gut check: testing a role for the intestinal microbiome in human obesity. Sci Transl Med. 2009 Nov 11; 1(6):6ps7.
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  39. Thanawastien A, Montor WR, Labaer J, Mekalanos JJ, Yoon SS. Vibrio cholerae proteome-wide screen for immunostimulatory proteins identifies phosphatidylserine decarboxylase as a novel Toll-like receptor 4 agonist. PLoS Pathog. 2009 Aug; 5(8):e1000556.
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  40. Ma AT, McAuley S, Pukatzki S, Mekalanos JJ. Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 2009 Mar 19; 5(3):234-43.
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  41. Leiman PG, Basler M, Ramagopal UA, Bonanno JB, Sauder JM, Pukatzki S, Burley SK, Almo SC, Mekalanos JJ. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009 Mar 17; 106(11):4154-9.
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  42. Zahid MS, Udden SM, Faruque AS, Calderwood SB, Mekalanos JJ, Faruque SM. Effect of phage on the infectivity of Vibrio cholerae and emergence of genetic variants. Infect Immun. 2008 Nov; 76(11):5266-73.
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  43. Udden SM, Zahid MS, Biswas K, Ahmad QS, Cravioto A, Nair GB, Mekalanos JJ, Faruque SM. Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence. Proc Natl Acad Sci U S A. 2008 Aug 19; 105(33):11951-6.
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  44. Rahman MH, Biswas K, Hossain MA, Sack RB, Mekalanos JJ, Faruque SM. Distribution of genes for virulence and ecological fitness among diverse Vibrio cholerae population in a cholera endemic area: tracking the evolution of pathogenic strains. DNA Cell Biol. 2008 Jul; 27(7):347-55.
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  45. Rhie GE, Jung HM, Kim BS, Mekalanos JJ. Construction of a Vibrio cholerae prototype vaccine strain O395-N1-E1 which accumulates cell-associated cholera toxin B subunit. Vaccine. 2008 Oct 9; 26(43):5443-8.
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  46. Cameron DE, Urbach JM, Mekalanos JJ. A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. Proc Natl Acad Sci U S A. 2008 Jun 24; 105(25):8736-41.
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  47. Rolfs A, Montor WR, Yoon SS, Hu Y, Bhullar B, Kelley F, McCarron S, Jepson DA, Shen B, Taycher E, Mohr SE, Zuo D, Williamson J, Mekalanos J, Labaer J. Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae. Proc Natl Acad Sci U S A. 2008 Mar 18; 105(11):4364-9.
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  48. Yoon SS, Mekalanos JJ. Decreased potency of the Vibrio cholerae sheathed flagellum to trigger host innate immunity. Infect Immun. 2008 Mar; 76(3):1282-8.
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  49. Rhie GE, Jung HM, Park J, Kim BS, Mekalanos JJ. Construction of cholera toxin B subunit-producing Vibrio cholerae strains using the Mariner-FRT transposon delivery system. FEMS Immunol Med Microbiol. 2008 Jan; 52(1):23-8.
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  50. Shakhnovich EA, Sturtevant D, Mekalanos JJ. Molecular mechanisms of virstatin resistance by non-O1/non-O139 strains of Vibrio cholerae. Mol Microbiol. 2007 Dec; 66(6):1331-41.
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  51. Pukatzki S, Ma AT, Revel AT, Sturtevant D, Mekalanos JJ. Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc Natl Acad Sci U S A. 2007 Sep 25; 104(39):15508-13.
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  52. Mougous JD, Gifford CA, Ramsdell TL, Mekalanos JJ. Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa. Nat Cell Biol. 2007 Jul; 9(7):797-803.
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  53. Tam VC, Serruto D, Dziejman M, Brieher W, Mekalanos JJ. A type III secretion system in Vibrio cholerae translocates a formin/spire hybrid-like actin nucleator to promote intestinal colonization. Cell Host Microbe. 2007 Apr 19; 1(2):95-107.
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  54. Faruque SM, Tam VC, Chowdhury N, Diraphat P, Dziejman M, Heidelberg JF, Clemens JD, Mekalanos JJ, Nair GB. Genomic analysis of the Mozambique strain of Vibrio cholerae O1 reveals the origin of El Tor strains carrying classical CTX prophage. Proc Natl Acad Sci U S A. 2007 Mar 20; 104(12):5151-6.
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  55. Raskin DM, Judson N, Mekalanos JJ. Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Proc Natl Acad Sci U S A. 2007 Mar 13; 104(11):4636-41.
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  56. Smith MG, Gianoulis TA, Pukatzki S, Mekalanos JJ, Ornston LN, Gerstein M, Snyder M. New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis. Genes Dev. 2007 Mar 1; 21(5):601-14.
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  57. Shakhnovich EA, Hung DT, Pierson E, Lee K, Mekalanos JJ. Virstatin inhibits dimerization of the transcriptional activator ToxT. Proc Natl Acad Sci U S A. 2007 Feb 13; 104(7):2372-7.
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  58. Yoon SS, Mekalanos JJ. 2,3-butanediol synthesis and the emergence of the Vibrio cholerae El Tor biotype. Infect Immun. 2006 Dec; 74(12):6547-56.
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  59. Qadri F, Chowdhury MI, Faruque SM, Salam MA, Ahmed T, Begum YA, Saha A, Al Tarique A, Seidlein LV, Park E, Killeen KP, Mekalanos JJ, Clemens JD, Sack DA. Peru-15, a live attenuated oral cholera vaccine, is safe and immunogenic in Bangladeshi toddlers and infants. Vaccine. 2007 Jan 4; 25(2):231-8.
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  60. Bina JE, Provenzano D, Wang C, Bina XR, Mekalanos JJ. Characterization of the Vibrio cholerae vexAB and vexCD efflux systems. Arch Microbiol. 2006 Sep; 186(3):171-81.
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  61. Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ. A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science. 2006 Jun 9; 312(5779):1526-30.
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  62. Faruque SM, Biswas K, Udden SM, Ahmad QS, Sack DA, Nair GB, Mekalanos JJ. Transmissibility of cholera: in vivo-formed biofilms and their relationship to infectivity and persistence in the environment. Proc Natl Acad Sci U S A. 2006 Apr 18; 103(16):6350-5.
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  63. Jensen MA, Faruque SM, Mekalanos JJ, Levin BR. Modeling the role of bacteriophage in the control of cholera outbreaks. Proc Natl Acad Sci U S A. 2006 Mar 21; 103(12):4652-7.
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  64. Raskin DM, Seshadri R, Pukatzki SU, Mekalanos JJ. Bacterial genomics and pathogen evolution. Cell. 2006 Feb 24; 124(4):703-14.
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  65. Faruque SM, Islam MJ, Ahmad QS, Biswas K, Faruque AS, Nair GB, Sack RB, Sack DA, Mekalanos JJ. An improved technique for isolation of environmental Vibrio cholerae with epidemic potential: monitoring the emergence of a multiple-antibiotic-resistant epidemic strain in Bangladesh. J Infect Dis. 2006 Apr 1; 193(7):1029-36.
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  66. Rietsch A, Mekalanos JJ. Metabolic regulation of type III secretion gene expression in Pseudomonas aeruginosa. Mol Microbiol. 2006 Feb; 59(3):807-20.
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  67. Pukatzki S, Ma AT, Sturtevant D, Krastins B, Sarracino D, Nelson WC, Heidelberg JF, Mekalanos JJ. Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 2006 Jan 31; 103(5):1528-33.
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  68. Hung DT, Zhu J, Sturtevant D, Mekalanos JJ. Bile acids stimulate biofilm formation in Vibrio cholerae. Mol Microbiol. 2006 Jan; 59(1):193-201.
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  69. Hung DT, Shakhnovich EA, Pierson E, Mekalanos JJ. Small-molecule inhibitor of Vibrio cholerae virulence and intestinal colonization. Science. 2005 Oct 28; 310(5748):670-4.
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  70. Larocque RC, Harris JB, Dziejman M, Li X, Khan AI, Faruque AS, Faruque SM, Nair GB, Ryan ET, Qadri F, Mekalanos JJ, Calderwood SB. Transcriptional profiling of Vibrio cholerae recovered directly from patient specimens during early and late stages of human infection. Infect Immun. 2005 Aug; 73(8):4488-93.
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  71. Qadri F, Chowdhury MI, Faruque SM, Salam MA, Ahmed T, Begum YA, Saha A, Alam MS, Zaman K, Seidlein LV, Park E, Killeen KP, Mekalanos JJ, Clemens JD, Sack DA. Randomized, controlled study of the safety and immunogenicity of Peru-15, a live attenuated oral vaccine candidate for cholera, in adult volunteers in Bangladesh. J Infect Dis. 2005 Aug 15; 192(4):573-9.
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  72. Aulinger BA, Roehrl MH, Mekalanos JJ, Collier RJ, Wang JY. Combining anthrax vaccine and therapy: a dominant-negative inhibitor of anthrax toxin is also a potent and safe immunogen for vaccines. Infect Immun. 2005 Jun; 73(6):3408-14.
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  73. Faruque SM, Bin Naser I, Fujihara K, Diraphat P, Chowdhury N, Kamruzzaman M, Qadri F, Yamasaki S, Ghosh AN, Mekalanos JJ. Genomic sequence and receptor for the Vibrio cholerae phage KSF-1phi: evolutionary divergence among filamentous vibriophages mediating lateral gene transfer. J Bacteriol. 2005 Jun; 187(12):4095-103.
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  74. Rietsch A, Vallet-Gely I, Dove SL, Mekalanos JJ. ExsE, a secreted regulator of type III secretion genes in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2005 May 31; 102(22):8006-11.
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  75. Faruque SM, Islam MJ, Ahmad QS, Faruque AS, Sack DA, Nair GB, Mekalanos JJ. Self-limiting nature of seasonal cholera epidemics: Role of host-mediated amplification of phage. Proc Natl Acad Sci U S A. 2005 Apr 26; 102(17):6119-24.
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  76. Altman S, Bassler BL, Beckwith J, Belfort M, Berg HC, Bloom B, Brenchley JE, Campbell A, Collier RJ, Connell N, Cozzarelli NR, Craig NL, Darst S, Ebright RH, Elledge SJ, Falkow S, Galan JE, Gottesman M, Gourse R, Grindley ND, Gross CA, Grossman A, Hochschild A, Howe M, Hurwitz J, Isberg RR, Kaplan S, Kornberg A, Kustu SG, Landick RC, Landy A, Levy SB, Losick R, Long SR, Maloy SR, Mekalanos JJ, Neidhardt FC, Pace NR, Ptashne M, Roberts JW, Roth JR, Rothman-Denes LB, Salyers A, Schaechter M, Shapiro L, Silhavy TJ, Simon MI, Walker G, Yanofsky C, Zinder N. An open letter to Elias Zerhouni. Science. 2005 Mar 4; 307(5714):1409-10.
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  77. Vance RE, Rietsch A, Mekalanos JJ. Role of the type III secreted exoenzymes S, T, and Y in systemic spread of Pseudomonas aeruginosa PAO1 in vivo. Infect Immun. 2005 Mar; 73(3):1706-13.
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  78. Dziejman M, Serruto D, Tam VC, Sturtevant D, Diraphat P, Faruque SM, Rahman MH, Heidelberg JF, Decker J, Li L, Montgomery KT, Grills G, Kucherlapati R, Mekalanos JJ. Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system. Proc Natl Acad Sci U S A. 2005 Mar 1; 102(9):3465-70.
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  79. Hung DT, Mekalanos JJ. Bile acids induce cholera toxin expression in Vibrio cholerae in a ToxT-independent manner. Proc Natl Acad Sci U S A. 2005 Feb 22; 102(8):3028-33.
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  80. Faruque SM, Naser IB, Islam MJ, Faruque AS, Ghosh AN, Nair GB, Sack DA, Mekalanos JJ. Seasonal epidemics of cholera inversely correlate with the prevalence of environmental cholera phages. Proc Natl Acad Sci U S A. 2005 Feb 1; 102(5):1702-7.
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  81. Faruque SM, Nair GB, Mekalanos JJ. Genetics of stress adaptation and virulence in toxigenic Vibrio cholerae. DNA Cell Biol. 2004 Nov; 23(11):723-41.
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  82. Rietsch A, Wolfgang MC, Mekalanos JJ. Effect of metabolic imbalance on expression of type III secretion genes in Pseudomonas aeruginosa. Infect Immun. 2004 Mar; 72(3):1383-90.
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  83. Jönsson K, Guo BP, Monstein HJ, Mekalanos JJ, Kronvall G. Molecular cloning and characterization of two Helicobacter pylori genes coding for plasminogen-binding proteins. Proc Natl Acad Sci U S A. 2004 Feb 17; 101(7):1852-7.
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  84. Faruque SM, Chowdhury N, Kamruzzaman M, Dziejman M, Rahman MH, Sack DA, Nair GB, Mekalanos JJ. Genetic diversity and virulence potential of environmental Vibrio cholerae population in a cholera-endemic area. Proc Natl Acad Sci U S A. 2004 Feb 17; 101(7):2123-8.
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  85. Vance RE, Hong S, Gronert K, Serhan CN, Mekalanos JJ. The opportunistic pathogen Pseudomonas aeruginosa carries a secretable arachidonate 15-lipoxygenase. Proc Natl Acad Sci U S A. 2004 Feb 17; 101(7):2135-9.
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  86. Faruque SM, Mekalanos JJ. Pathogenicity islands and phages in Vibrio cholerae evolution. Trends Microbiol. 2003 Nov; 11(11):505-10.
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  87. Zhu J, Mekalanos JJ. Quorum sensing-dependent biofilms enhance colonization in Vibrio cholerae. Dev Cell. 2003 Oct; 5(4):647-56.
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  88. Rhie GE, Roehrl MH, Mourez M, Collier RJ, Mekalanos JJ, Wang JY. A dually active anthrax vaccine that confers protection against both bacilli and toxins. Proc Natl Acad Sci U S A. 2003 Sep 16; 100(19):10925-30.
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  89. Faruque SM, Zhu J, Kamruzzaman M, Mekalanos JJ. Examination of diverse toxin-coregulated pilus-positive Vibrio cholerae strains fails to demonstrate evidence for Vibrio pathogenicity island phage. Infect Immun. 2003 Jun; 71(6):2993-9.
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  90. Vance RE, Zhu J, Mekalanos JJ. A constitutively active variant of the quorum-sensing regulator LuxO affects protease production and biofilm formation in Vibrio cholerae. Infect Immun. 2003 May; 71(5):2571-6.
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  91. Bina J, Zhu J, Dziejman M, Faruque S, Calderwood S, Mekalanos J. ToxR regulon of Vibrio cholerae and its expression in vibrios shed by cholera patients. Proc Natl Acad Sci U S A. 2003 Mar 4; 100(5):2801-6.
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  92. Xu Q, Dziejman M, Mekalanos JJ. Determination of the transcriptome of Vibrio cholerae during intraintestinal growth and midexponential phase in vitro. Proc Natl Acad Sci U S A. 2003 Feb 4; 100(3):1286-91.
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  93. Faruque SM, Kamruzzaman M, Sack DA, Mekalanos JJ, Nair GB. CTXphi-independent production of the RS1 satellite phage by Vibrio cholerae. Proc Natl Acad Sci U S A. 2003 Feb 4; 100(3):1280-5.
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  94. Guo BP, Mekalanos JJ. Rapid genetic analysis of Helicobacter pylori gastric mucosal colonization in suckling mice. Proc Natl Acad Sci U S A. 2002 Jun 11; 99(12):8354-9.
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  95. Fullner KJ, Boucher JC, Hanes MA, Haines GK, Meehan BM, Walchle C, Sansonetti PJ, Mekalanos JJ. The contribution of accessory toxins of Vibrio cholerae O1 El Tor to the proinflammatory response in a murine pulmonary cholera model. J Exp Med. 2002 Jun 3; 195(11):1455-62.
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  96. Buddelmeijer N, Judson N, Boyd D, Mekalanos JJ, Beckwith J. YgbQ, a cell division protein in Escherichia coli and Vibrio cholerae, localizes in codependent fashion with FtsL to the division site. Proc Natl Acad Sci U S A. 2002 Apr 30; 99(9):6316-21.
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  97. Pukatzki S, Kessin RH, Mekalanos JJ. The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum. Proc Natl Acad Sci U S A. 2002 Mar 5; 99(5):3159-64.
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  98. Zhu J, Miller MB, Vance RE, Dziejman M, Bassler BL, Mekalanos JJ. Quorum-sensing regulators control virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A. 2002 Mar 5; 99(5):3129-34.
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  99. Dziejman M, Balon E, Boyd D, Fraser CM, Heidelberg JF, Mekalanos JJ. Comparative genomic analysis of Vibrio cholerae: genes that correlate with cholera endemic and pandemic disease. Proc Natl Acad Sci U S A. 2002 Feb 5; 99(3):1556-61.
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  100. Akerley BJ, Rubin EJ, Novick VL, Amaya K, Judson N, Mekalanos JJ. A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A. 2002 Jan 22; 99(2):966-71.
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  101. Faruque SM, Kamruzzaman M, Nandi RK, Ghosh AN, Nair GB, Mekalanos JJ, Sack DA. RS1 element of Vibrio cholerae can propagate horizontally as a filamentous phage exploiting the morphogenesis genes of CTXphi. Infect Immun. 2002 Jan; 70(1):163-70.
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  102. Dalsgaard A, Serichantalergs O, Forslund A, Lin W, Mekalanos J, Mintz E, Shimada T, Wells JG. Clinical and environmental isolates of Vibrio cholerae serogroup O141 carry the CTX phage and the genes encoding the toxin-coregulated pili. J Clin Microbiol. 2001 Nov; 39(11):4086-92.
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  103. Fullner KJ, Lencer WI, Mekalanos JJ. Vibrio cholerae-induced cellular responses of polarized T84 intestinal epithelial cells are dependent on production of cholera toxin and the RTX toxin. Infect Immun. 2001 Oct; 69(10):6310-7.
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  104. Faruque SM, Rahman MM, Hasan AK, Nair GB, Mekalanos JJ, Sack DA. Diminished diarrheal response to Vibrio cholerae strains carrying the replicative form of the CTX(Phi) genome instead of CTX(Phi) lysogens in adult rabbits. Infect Immun. 2001 Oct; 69(10):6084-90.
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  105. Bina JE, Mekalanos JJ. Vibrio cholerae tolC is required for bile resistance and colonization. Infect Immun. 2001 Jul; 69(7):4681-5.
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  106. Guo BP, Mekalanos JJ. Helicobacter pylori mutagenesis by mariner in vitro transposition. FEMS Immunol Med Microbiol. 2001 Mar; 30(2):87-93.
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  107. Cassell GH, Mekalanos J. Development of antimicrobial agents in the era of new and reemerging infectious diseases and increasing antibiotic resistance. JAMA. 2001 Feb 7; 285(5):601-5.
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  108. Judson N, Mekalanos JJ. Transposon-based approaches to identify essential bacterial genes. Trends Microbiol. 2000 Nov; 8(11):521-6.
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  109. Mel SF, Fullner KJ, Wimer-Mackin S, Lencer WI, Mekalanos JJ. Association of protease activity in Vibrio cholerae vaccine strains with decreases in transcellular epithelial resistance of polarized T84 intestinal epithelial cells. Infect Immun. 2000 Nov; 68(11):6487-92.
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  110. Chiang SL, Mekalanos JJ. Construction of a Vibrio cholerae vaccine candidate using transposon delivery and FLP recombinase-mediated excision. Infect Immun. 2000 Nov; 68(11):6391-7.
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  111. Fullner KJ, Mekalanos JJ. In vivo covalent cross-linking of cellular actin by the Vibrio cholerae RTX toxin. EMBO J. 2000 Oct 16; 19(20):5315-23.
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  112. Wong SM, Mekalanos JJ. Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2000 Aug 29; 97(18):10191-6.
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  113. Heidelberg JF, Eisen JA, Nelson WC, Clayton RA, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Umayam L, Gill SR, Nelson KE, Read TD, Tettelin H, Richardson D, Ermolaeva MD, Vamathevan J, Bass S, Qin H, Dragoi I, Sellers P, McDonald L, Utterback T, Fleishmann RD, Nierman WC, White O, Salzberg SL, Smith HO, Colwell RR, Mekalanos JJ, Venter JC, Fraser CM. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature. 2000 Aug 3; 406(6795):477-83.
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  114. Keen N, Staskawicz B, Mekalanos J, Ausubel F, Cook RJ. Pathogens and hosts: the dance is the same, the couples are different. Proc Natl Acad Sci U S A. 2000 Aug 1; 97(16):8752-3.
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  115. Judson N, Mekalanos JJ. TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes. Nat Biotechnol. 2000 Jul; 18(7):740-5.
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  116. Blum JH, Dove SL, Hochschild A, Mekalanos JJ. Isolation of peptide aptamers that inhibit intracellular processes. Proc Natl Acad Sci U S A. 2000 Feb 29; 97(5):2241-6.
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  117. Jain V, Mekalanos JJ. Use of lambda phage S and R gene products in an inducible lysis system for Vibrio cholerae- and Salmonella enterica serovar typhimurium-based DNA vaccine delivery systems. Infect Immun. 2000 Feb; 68(2):986-9.
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  118. Faruque SM, Rahman MM, Nasirul Islam KM, Mekalanos JJ. Lysogenic conversion of environmental Vibrio mimicus strains by CTXPhi. Infect Immun. 1999 Nov; 67(11):5723-9.
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  119. Lampe DJ, Akerley BJ, Rubin EJ, Mekalanos JJ, Robertson HM. Hyperactive transposase mutants of the Himar1 mariner transposon. Proc Natl Acad Sci U S A. 1999 Sep 28; 96(20):11428-33.
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  120. Häse CC, Mekalanos JJ. Effects of changes in membrane sodium flux on virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A. 1999 Mar 16; 96(6):3183-7.
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  121. Fullner KJ, Mekalanos JJ. Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. Infect Immun. 1999 Mar; 67(3):1393-404.
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  122. Rubin EJ, Akerley BJ, Novik VN, Lampe DJ, Husson RN, Mekalanos JJ. In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria. Proc Natl Acad Sci U S A. 1999 Feb 16; 96(4):1645-50.
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  123. Lin W, Fullner KJ, Clayton R, Sexton JA, Rogers MB, Calia KE, Calderwood SB, Fraser C, Mekalanos JJ. Identification of a vibrio cholerae RTX toxin gene cluster that is tightly linked to the cholera toxin prophage. Proc Natl Acad Sci U S A. 1999 Feb 2; 96(3):1071-6.
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  124. Chiang SL, Mekalanos JJ. rfb mutations in Vibrio cholerae do not affect surface production of toxin-coregulated pili but still inhibit intestinal colonization. Infect Immun. 1999 Feb; 67(2):976-80.
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  125. Dziejman M, Kolmar H, Fritz HJ, Mekalanos JJ. ToxR co-operative interactions are not modulated by environmental conditions or periplasmic domain conformation. Mol Microbiol. 1999 Jan; 31(1):305-17.
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  126. Killeen K, Spriggs D, Mekalanos J. Bacterial mucosal vaccines: Vibrio cholerae as a live attenuated vaccine/vector paradigm. Curr Top Microbiol Immunol. 1999; 236:237-54.
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  127. Chiang SL, Mekalanos JJ, Holden DW. In vivo genetic analysis of bacterial virulence. Annu Rev Microbiol. 1999; 53:129-54.
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  128. Faruque SM, Albert MJ, Mekalanos JJ. Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae. Microbiol Mol Biol Rev. 1998 Dec; 62(4):1301-14.
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  129. Faruque SM, Saha MN, Alim AR, Albert MJ, Islam KM, Mekalanos JJ. Analysis of clinical and environmental strains of nontoxigenic Vibrio cholerae for susceptibility to CTXPhi: molecular basis for origination of new strains with epidemic potential. Infect Immun. 1998 Dec; 66(12):5819-25.
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  130. Meno Y, Waldor MK, Mekalanos JJ, Amako K. Morphological and physical characterization of the capsular layer of Vibrio cholerae O139. Arch Microbiol. 1998 Oct; 170(5):339-44.
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  131. Klose KE, Novik V, Mekalanos JJ. Identification of multiple sigma54-dependent transcriptional activators in Vibrio cholerae. J Bacteriol. 1998 Oct; 180(19):5256-9.
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  132. Acheson DW, Reidl J, Zhang X, Keusch GT, Mekalanos JJ, Waldor MK. In vivo transduction with shiga toxin 1-encoding phage. Infect Immun. 1998 Sep; 66(9):4496-8.
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  133. Faruque SM, Alim AR, Albert MJ, Islam KM, Mekalanos JJ. Induction of the lysogenic phage encoding cholera toxin in naturally occurring strains of toxigenic Vibrio cholerae O1 and O139. Infect Immun. 1998 Aug; 66(8):3752-7.
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  134. Akerley BJ, Rubin EJ, Camilli A, Lampe DJ, Robertson HM, Mekalanos JJ. Systematic identification of essential genes by in vitro mariner mutagenesis. Proc Natl Acad Sci U S A. 1998 Jul 21; 95(15):8927-32.
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  135. Rubin EJ, Lin W, Mekalanos JJ, Waldor MK. Replication and integration of a Vibrio cholerae cryptic plasmid linked to the CTX prophage. Mol Microbiol. 1998 Jun; 28(6):1247-54.
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  136. Martinez de Tejada G, Cotter PA, Heininger U, Camilli A, Akerley BJ, Mekalanos JJ, Miller JF. Neither the Bvg- phase nor the vrg6 locus of Bordetella pertussis is required for respiratory infection in mice. Infect Immun. 1998 Jun; 66(6):2762-8.
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  137. Lee SH, Angelichio MJ, Mekalanos JJ, Camilli A. Nucleotide sequence and spatiotemporal expression of the Vibrio cholerae vieSAB genes during infection. J Bacteriol. 1998 May; 180(9):2298-305.
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  138. Klose KE, Mekalanos JJ. Distinct roles of an alternative sigma factor during both free-swimming and colonizing phases of the Vibrio cholerae pathogenic cycle. Mol Microbiol. 1998 May; 28(3):501-20.
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  139. Chiang SL, Mekalanos JJ. Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol Microbiol. 1998 Feb; 27(4):797-805.
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  140. Häse CC, Mekalanos JJ. TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A. 1998 Jan 20; 95(2):730-4.
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  141. Klose KE, Mekalanos JJ. Differential regulation of multiple flagellins in Vibrio cholerae. J Bacteriol. 1998 Jan; 180(2):303-16.
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  142. Mekalanos JJ, Rubin EJ, Waldor MK. Cholera: molecular basis for emergence and pathogenesis. FEMS Immunol Med Microbiol. 1997 Aug; 18(4):241-8.
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  143. Waldor MK, Rubin EJ, Pearson GD, Kimsey H, Mekalanos JJ. Regulation, replication, and integration functions of the Vibrio cholerae CTXphi are encoded by region RS2. Mol Microbiol. 1997 Jun; 24(5):917-26.
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  144. Klose KE, Mekalanos JJ. Simultaneous prevention of glutamine synthesis and high-affinity transport attenuates Salmonella typhimurium virulence. Infect Immun. 1997 Feb; 65(2):587-96.
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  145. Mel SF, Mekalanos JJ. Modulation of horizontal gene transfer in pathogenic bacteria by in vivo signals. Cell. 1996 Nov 29; 87(5):795-8.
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  146. Slauch JM, Lee AA, Mahan MJ, Mekalanos JJ. Molecular characterization of the oafA locus responsible for acetylation of Salmonella typhimurium O-antigen: oafA is a member of a family of integral membrane trans-acylases. J Bacteriol. 1996 Oct; 178(20):5904-9.
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  147. Waldor MK, Tschäpe H, Mekalanos JJ. A new type of conjugative transposon encodes resistance to sulfamethoxazole, trimethoprim, and streptomycin in Vibrio cholerae O139. J Bacteriol. 1996 Jul; 178(14):4157-65.
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  148. Waldor MK, Mekalanos JJ. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science. 1996 Jun 28; 272(5270):1910-4.
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  149. Gardel CL, Mekalanos JJ. Alterations in Vibrio cholerae motility phenotypes correlate with changes in virulence factor expression. Infect Immun. 1996 Jun; 64(6):2246-55.
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  150. Reidl J, Mekalanos JJ. Lipoprotein e(P4) is essential for hemin uptake by Haemophilus influenzae. J Exp Med. 1996 Feb 1; 183(2):621-9.
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  151. Ottemann KM, Mekalanos JJ. The ToxR protein of Vibrio cholerae forms homodimers and heterodimers. J Bacteriol. 1996 Jan; 178(1):156-62.
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  152. Carter RM, Mekalanos JJ, Jacobs MB, Lubrano GJ, Guilbault GG. Quartz crystal microbalance detection of Vibrio cholerae O139 serotype. J Immunol Methods. 1995 Nov 16; 187(1):121-5.
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  153. Camilli A, Mekalanos JJ. Use of recombinase gene fusions to identify Vibrio cholerae genes induced during infection. Mol Microbiol. 1995 Nov; 18(4):671-83.
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  154. Reidl J, Mekalanos JJ. Characterization of Vibrio cholerae bacteriophage K139 and use of a novel mini-transposon to identify a phage-encoded virulence factor. Mol Microbiol. 1995 Nov; 18(4):685-701.
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  155. Chiang SL, Taylor RK, Koomey M, Mekalanos JJ. Single amino acid substitutions in the N-terminus of Vibrio cholerae TcpA affect colonization, autoagglutination, and serum resistance. Mol Microbiol. 1995 Sep; 17(6):1133-42.
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  156. Butterton JR, Beattie DT, Gardel CL, Carroll PA, Hyman T, Killeen KP, Mekalanos JJ, Calderwood SB. Heterologous antigen expression in Vibrio cholerae vector strains. Infect Immun. 1995 Jul; 63(7):2689-96.
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  157. Coster TS, Killeen KP, Waldor MK, Beattie DT, Spriggs DR, Kenner JR, Trofa A, Sadoff JC, Mekalanos JJ, Taylor DN. Safety, immunogenicity, and efficacy of live attenuated Vibrio cholerae O139 vaccine prototype. Lancet. 1995 Apr 15; 345(8955):949-52.
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  158. Ottemann KM, Mekalanos JJ. Analysis of Vibrio cholerae ToxR function by construction of novel fusion proteins. Mol Microbiol. 1995 Feb; 15(4):719-31.
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  159. Slauch JM, Mahan MJ, Michetti P, Neutra MR, Mekalanos JJ. Acetylation (O-factor 5) affects the structural and immunological properties of Salmonella typhimurium lipopolysaccharide O antigen. Infect Immun. 1995 Feb; 63(2):437-41.
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  160. Mahan MJ, Tobias JW, Slauch JM, Hanna PC, Collier RJ, Mekalanos JJ. Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc Natl Acad Sci U S A. 1995 Jan 31; 92(3):669-73.
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  161. Waldor MK, Colwell R, Mekalanos JJ. The Vibrio cholerae O139 serogroup antigen includes an O-antigen capsule and lipopolysaccharide virulence determinants. Proc Natl Acad Sci U S A. 1994 Nov 22; 91(24):11388-92.
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  162. Michetti P, Porta N, Mahan MJ, Slauch JM, Mekalanos JJ, Blum AL, Kraehenbuhl JP, Neutra MR. Monoclonal immunoglobulin A prevents adherence and invasion of polarized epithelial cell monolayers by Salmonella typhimurium. Gastroenterology. 1994 Oct; 107(4):915-23.
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  163. Mekalanos JJ, Sadoff JC. Cholera vaccines: fighting an ancient scourge. Science. 1994 Sep 2; 265(5177):1387-9.
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  164. Dziejman M, Mekalanos JJ. Analysis of membrane protein interaction: ToxR can dimerize the amino terminus of phage lambda repressor. Mol Microbiol. 1994 Aug; 13(3):485-94.
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  165. Waldor MK, Mekalanos JJ. Emergence of a new cholera pandemic: molecular analysis of virulence determinants in Vibrio cholerae O139 and development of a live vaccine prototype. J Infect Dis. 1994 Aug; 170(2):278-83.
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  166. Mekalanos JJ. Live bacterial vaccines: environmental aspects. Curr Opin Biotechnol. 1994 Jun; 5(3):312-9.
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  167. Waldor MK, Mekalanos JJ. Vibrio cholerae O139 specific gene sequences. Lancet. 1994 May 28; 343(8909):1366.
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  168. Slauch JM, Mahan MJ, Mekalanos JJ. Measurement of transcriptional activity in pathogenic bacteria recovered directly from infected host tissue. Biotechniques. 1994 Apr; 16(4):641-4.
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  169. Camilli A, Beattie DT, Mekalanos JJ. Use of genetic recombination as a reporter of gene expression. Proc Natl Acad Sci U S A. 1994 Mar 29; 91(7):2634-8.
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  170. Slauch JM, Mahan MJ, Mekalanos JJ. In vivo expression technology for selection of bacterial genes specifically induced in host tissues. Methods Enzymol. 1994; 235:481-92.
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  171. Gardel CL, Mekalanos JJ. Regulation of cholera toxin by temperature, pH, and osmolarity. Methods Enzymol. 1994; 235:517-26.
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  172. Mekalanos JJ. Live attenuated vaccine vectors. Int J Technol Assess Health Care. 1994; 10(1):131-42.
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  173. Waldor MK, Mekalanos JJ. ToxR regulates virulence gene expression in non-O1 strains of Vibrio cholerae that cause epidemic cholera. Infect Immun. 1994 Jan; 62(1):72-8.
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  174. Apter FM, Lencer WI, Finkelstein RA, Mekalanos JJ, Neutra MR. Monoclonal immunoglobulin A antibodies directed against cholera toxin prevent the toxin-induced chloride secretory response and block toxin binding to intestinal epithelial cells in vitro. Infect Immun. 1993 Dec; 61(12):5271-8.
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  175. Apter FM, Michetti P, Winner LS, Mack JA, Mekalanos JJ, Neutra MR. Analysis of the roles of antilipopolysaccharide and anti-cholera toxin immunoglobulin A (IgA) antibodies in protection against Vibrio cholerae and cholera toxin by use of monoclonal IgA antibodies in vivo. Infect Immun. 1993 Dec; 61(12):5279-85.
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  176. Mahan MJ, Slauch JM, Mekalanos JJ. Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions. J Bacteriol. 1993 Nov; 175(21):7086-91.
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  177. Mahan MJ, Slauch JM, Hanna PC, Camilli A, Tobias JW, Waldor MK, Mekalanos JJ. Selection for bacterial genes that are specifically induced in host tissues: the hunt for virulence factors. Infect Agents Dis. 1993 Aug; 2(4):263-8.
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  178. Lin Z, Kumagai K, Baba K, Mekalanos JJ, Nishibuchi M. Vibrio parahaemolyticus has a homolog of the Vibrio cholerae toxRS operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. J Bacteriol. 1993 Jun; 175(12):3844-55.
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  179. Pearson GD, Woods A, Chiang SL, Mekalanos JJ. CTX genetic element encodes a site-specific recombination system and an intestinal colonization factor. Proc Natl Acad Sci U S A. 1993 Apr 15; 90(8):3750-4.
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  180. Mahan MJ, Slauch JM, Mekalanos JJ. Selection of bacterial virulence genes that are specifically induced in host tissues. Science. 1993 Jan 29; 259(5095):686-8.
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  181. Beattie DT, Mahan MJ, Mekalanos JJ. Repressor binding to a regulatory site in the DNA coding sequence is sufficient to confer transcriptional regulation of the vir-repressed genes (vrg genes) in Bordetella pertussis. J Bacteriol. 1993 Jan; 175(2):519-27.
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  183. Ottemann KM, DiRita VJ, Mekalanos JJ. ToxR proteins with substitutions in residues conserved with OmpR fail to activate transcription from the cholera toxin promoter. J Bacteriol. 1992 Nov; 174(21):6807-14.
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  184. Parsot C, Mekalanos JJ. Structural analysis of the acfA and acfD genes of Vibrio cholerae: effects of DNA topology and transcriptional activators on expression. J Bacteriol. 1992 Aug; 174(16):5211-8.
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  185. Killeen KP, Escuyer V, Mekalanos JJ, Collier RJ. Reversion of recombinant toxoids: mutations in diphtheria toxin that partially compensate for active-site deletions. Proc Natl Acad Sci U S A. 1992 Jul 1; 89(13):6207-9.
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  186. Michetti P, Mahan MJ, Slauch JM, Mekalanos JJ, Neutra MR. Monoclonal secretory immunoglobulin A protects mice against oral challenge with the invasive pathogen Salmonella typhimurium. Infect Immun. 1992 May; 60(5):1786-92.
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  187. Beattie DT, Shahin R, Mekalanos JJ. A vir-repressed gene of Bordetella pertussis is required for virulence. Infect Immun. 1992 Feb; 60(2):571-7.
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  188. Miller JF, Johnson SA, Black WJ, Beattie DT, Mekalanos JJ, Falkow S. Constitutive sensory transduction mutations in the Bordetella pertussis bvgS gene. J Bacteriol. 1992 Feb; 174(3):970-9.
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  189. Mekalanos JJ. Bacterial mucosal vaccines. Adv Exp Med Biol. 1992; 327:43-50.
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  191. Finn TM, Shahin R, Mekalanos JJ. Characterization of vir-activated TnphoA gene fusions in Bordetella pertussis. Infect Immun. 1991 Sep; 59(9):3273-9.
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  192. Hall RH, Losonsky G, Silveira AP, Taylor RK, Mekalanos JJ, Witham ND, Levine MM. Immunogenicity of Vibrio cholerae O1 toxin-coregulated pili in experimental and clinical cholera. Infect Immun. 1991 Jul; 59(7):2508-12.
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  193. DiRita VJ, Parsot C, Jander G, Mekalanos JJ. Regulatory cascade controls virulence in Vibrio cholerae. Proc Natl Acad Sci U S A. 1991 Jun 15; 88(12):5403-7.
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  194. Parsot C, Mekalanos JJ. Expression of the Vibrio cholerae gene encoding aldehyde dehydrogenase is under control of ToxR, the cholera toxin transcriptional activator. J Bacteriol. 1991 May; 173(9):2842-51.
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  195. Parsot C, Taxman E, Mekalanos JJ. ToxR regulates the production of lipoproteins and the expression of serum resistance in Vibrio cholerae. Proc Natl Acad Sci U S A. 1991 Mar 1; 88(5):1641-5.
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  196. Winner L, Mack J, Weltzin R, Mekalanos JJ, Kraehenbuhl JP, Neutra MR. New model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against Vibrio cholerae infection. Infect Immun. 1991 Mar; 59(3):977-82.
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  197. DiRita VJ, Mekalanos JJ. Periplasmic interaction between two membrane regulatory proteins, ToxR and ToxS, results in signal transduction and transcriptional activation. Cell. 1991 Jan 11; 64(1):29-37.
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  198. Neutra M, Weltzin R, Winner L, Mack J, Michetti P, Morrison L, Fields BN, Mekalanos JJ, Kraehenbuhl JP. Identification and use of protective monoclonal IgA antibodies against viral and bacterial pathogens. Adv Exp Med Biol. 1991; 310:179-82.
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  199. Parsot C, Mekalanos JJ. Expression of ToxR, the transcriptional activator of the virulence factors in Vibrio cholerae, is modulated by the heat shock response. Proc Natl Acad Sci U S A. 1990 Dec; 87(24):9898-902.
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  200. Beattie DT, Knapp S, Mekalanos JJ. Evidence that modulation requires sequences downstream of the promoters of two vir-repressed genes of Bordetella pertussis. J Bacteriol. 1990 Dec; 172(12):6997-7004.
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  201. Miller SI, Pulkkinen WS, Selsted ME, Mekalanos JJ. Characterization of defensin resistance phenotypes associated with mutations in the phoP virulence regulon of Salmonella typhimurium. Infect Immun. 1990 Nov; 58(11):3706-10.
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  202. Miller SI, Mekalanos JJ, Pulkkinen WS. Salmonella vaccines with mutations in the phoP virulence regulon. Res Microbiol. 1990 Sep-Oct; 141(7-8):817-21.
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  203. Pearson GD, DiRita VJ, Goldberg MB, Boyko SA, Calderwood SB, Mekalanos JJ. New attenuated derivatives of Vibrio cholerae. Res Microbiol. 1990 Sep-Oct; 141(7-8):893-9.
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  204. Lee CK, Roberts AL, Finn TM, Knapp S, Mekalanos JJ. A new assay for invasion of HeLa 229 cells by Bordetella pertussis: effects of inhibitors, phenotypic modulation, and genetic alterations. Infect Immun. 1990 Aug; 58(8):2516-22.
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  205. Soltis MT, Mekalanos JJ, Betley MJ. Identification of a bacteriophage containing a silent staphylococcal variant enterotoxin gene (sezA+). Infect Immun. 1990 Jun; 58(6):1614-9.
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  206. Sun DX, Mekalanos JJ, Taylor RK. Antibodies directed against the toxin-coregulated pilus isolated from Vibrio cholerae provide protection in the infant mouse experimental cholera model. J Infect Dis. 1990 Jun; 161(6):1231-6.
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  207. Miller SI, Mekalanos JJ. Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages. J Bacteriol. 1990 May; 172(5):2485-90.
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  208. Manoil C, Mekalanos JJ, Beckwith J. Alkaline phosphatase fusions: sensors of subcellular location. J Bacteriol. 1990 Feb; 172(2):515-8.
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  209. Miller SI, Kukral AM, Mekalanos JJ. A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence. Proc Natl Acad Sci U S A. 1989 Jul; 86(13):5054-8.
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  210. Taylor RK, Manoil C, Mekalanos JJ. Broad-host-range vectors for delivery of TnphoA: use in genetic analysis of secreted virulence determinants of Vibrio cholerae. J Bacteriol. 1989 Apr; 171(4):1870-8.
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  211. Miller VL, DiRita VJ, Mekalanos JJ. Identification of toxS, a regulatory gene whose product enhances toxR-mediated activation of the cholera toxin promoter. J Bacteriol. 1989 Mar; 171(3):1288-93.
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  212. Miller JF, Mekalanos JJ, Falkow S. Coordinate regulation and sensory transduction in the control of bacterial virulence. Science. 1989 Feb 17; 243(4893):916-22.
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  213. DiRita VJ, Mekalanos JJ. Genetic regulation of bacterial virulence. Annu Rev Genet. 1989; 23:455-82.
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  214. Miller SI, Mekalanos JJ. Strategies for the development of vaccines for typhoid fever, shigellosis, and cholera. Ann N Y Acad Sci. 1989; 569:145-54.
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  215. Knapp S, Mekalanos JJ. Two trans-acting regulatory genes (vir and mod) control antigenic modulation in Bordetella pertussis. J Bacteriol. 1988 Nov; 170(11):5059-66.
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  216. Peterson KM, Mekalanos JJ. Characterization of the Vibrio cholerae ToxR regulon: identification of novel genes involved in intestinal colonization. Infect Immun. 1988 Nov; 56(11):2822-9.
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  217. Herrington DA, Hall RH, Losonsky G, Mekalanos JJ, Taylor RK, Levine MM. Toxin, toxin-coregulated pili, and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans. J Exp Med. 1988 Oct 1; 168(4):1487-92.
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  218. Miller VL, Mekalanos JJ. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol. 1988 Jun; 170(6):2575-83.
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  219. Taylor R, Shaw C, Peterson K, Spears P, Mekalanos J. Safe, live Vibrio cholerae vaccines? Vaccine. 1988 Apr; 6(2):151-4.
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  220. Hovde CJ, Calderwood SB, Mekalanos JJ, Collier RJ. Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I. Proc Natl Acad Sci U S A. 1988 Apr; 85(8):2568-72.
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  221. Hall RH, Vial PA, Kaper JB, Mekalanos JJ, Levine MM. Morphological studies on fimbriae expressed by Vibrio cholerae 01. Microb Pathog. 1988 Apr; 4(4):257-65.
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  222. Calderwood SB, Mekalanos JJ. Confirmation of the Fur operator site by insertion of a synthetic oligonucleotide into an operon fusion plasmid. J Bacteriol. 1988 Feb; 170(2):1015-7.
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  223. Mekalanos JJ, Peterson KM, Finn T, Knapp S. The pathogenesis and immunology of Vibrio cholerae and Bordetella pertussis. Antonie Van Leeuwenhoek. 1988; 54(5):379-87.
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  224. Pierce NF, Cray WC, Kaper JB, Mekalanos JJ. Determinants of immunogenicity and mechanisms of protection by virulent and mutant Vibrio cholerae O1 in rabbits. Infect Immun. 1988 Jan; 56(1):142-8.
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  225. Mekalanos JJ. Production and purification of cholera toxin. Methods Enzymol. 1988; 165:169-75.
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  226. Betley MJ, Mekalanos JJ. Nucleotide sequence of the type A staphylococcal enterotoxin gene. J Bacteriol. 1988 Jan; 170(1):34-41.
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  227. Chopra AK, Houston CW, Peterson JW, Prasad R, Mekalanos JJ. Cloning and expression of the Salmonella enterotoxin gene. J Bacteriol. 1987 Nov; 169(11):5095-100.
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  228. Calderwood SB, Mekalanos JJ. Iron regulation of Shiga-like toxin expression in Escherichia coli is mediated by the fur locus. J Bacteriol. 1987 Oct; 169(10):4759-64.
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  229. Calderwood SB, Auclair F, Donohue-Rolfe A, Keusch GT, Mekalanos JJ. Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. Proc Natl Acad Sci U S A. 1987 Jul; 84(13):4364-8.
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  230. Taylor RK, Miller VL, Furlong DB, Mekalanos JJ. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci U S A. 1987 May; 84(9):2833-7.
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  231. Pierce NF, Kaper JB, Mekalanos JJ, Cray WC, Richardson K. Determinants of the immunogenicity of live virulent and mutant Vibrio cholerae O1 in rabbit intestine. Infect Immun. 1987 Feb; 55(2):477-81.
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  232. Miller VL, Taylor RK, Mekalanos JJ. Cholera toxin transcriptional activator toxR is a transmembrane DNA binding protein. Cell. 1987 Jan 30; 48(2):271-9.
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  233. Goldberg I, Mekalanos JJ. Effect of a recA mutation on cholera toxin gene amplification and deletion events. J Bacteriol. 1986 Mar; 165(3):723-31.
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  234. Goldberg I, Mekalanos JJ. Cloning of the Vibrio cholerae recA gene and construction of a Vibrio cholerae recA mutant. J Bacteriol. 1986 Mar; 165(3):715-22.
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  235. Taylor RK, Miller VL, Furlong DB, Mekalanos JJ. Identification of a pilus colonization factor that is coordinately regulated with cholera toxin. Ann Sclavo Collana Monogr. 1986; 3(1-2):51-61.
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  236. Betley MJ, Miller VL, Mekalanos JJ. Genetics of bacterial enterotoxins. Annu Rev Microbiol. 1986; 40:577-605.
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  237. Pierce NF, Kaper JB, Mekalanos JJ, Cray WC. Role of cholera toxin in enteric colonization by Vibrio cholerae O1 in rabbits. Infect Immun. 1985 Dec; 50(3):813-6.
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  238. Miller VL, Mekalanos JJ. Genetic analysis of the cholera toxin-positive regulatory gene toxR. J Bacteriol. 1985 Aug; 163(2):580-5.
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  239. Betley MJ, Mekalanos JJ. Staphylococcal enterotoxin A is encoded by phage. Science. 1985 Jul 12; 229(4709):185-7.
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  240. Watkins PA, Yost DA, Chang AW, Mekalanos JJ, Moss J. Detection of NAD:arginine ADPribosyltransferases in animal tissues using 125I-labeled 1-(p-hydroxyphenyl) 2-guanidinoethane as ADPribose acceptor. Biochim Biophys Acta. 1985 Jul 5; 840(3):401-8.
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  241. Mekalanos JJ. Cholera toxin: genetic analysis, regulation, and role in pathogenesis. Curr Top Microbiol Immunol. 1985; 118:97-118.
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  242. Miller VL, Mekalanos JJ. Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR. Proc Natl Acad Sci U S A. 1984 Jun; 81(11):3471-5.
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  243. Sporecke I, Castro D, Mekalanos JJ. Genetic mapping of Vibrio cholerae enterotoxin structural genes. J Bacteriol. 1984 Jan; 157(1):253-61.
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  244. Mekalanos JJ, Swartz DJ, Pearson GD, Harford N, Groyne F, de Wilde M. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature. 1983 Dec 8-14; 306(5943):551-7.
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  245. Mekalanos JJ. Duplication and amplification of toxin genes in Vibrio cholerae. Cell. 1983 Nov; 35(1):253-63.
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  246. Pearson GD, Mekalanos JJ. Molecular cloning of Vibrio cholerae enterotoxin genes in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1982 May; 79(9):2976-80.
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  247. Mekalanos JJ, Moseley SL, Murphy JR, Falkow S. Isolation of enterotoxin structural gene deletion mutations in Vibrio cholerae induced by two mutagenic vibriophages. Proc Natl Acad Sci U S A. 1982 Jan; 79(1):151-5.
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  248. Mekalanos JJ, Murphy JR. Regulation of cholera toxin production in Vibrio cholerae: genetic analysis of phenotypic instability in hypertoxinogenic mutants. J Bacteriol. 1980 Feb; 141(2):570-6.
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  249. Mekalanos JJ, Sublett RD, Romig WR. Genetic mapping of toxin regulatory mutations in Vibrio cholerae. J Bacteriol. 1979 Sep; 139(3):859-65.
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  250. Mekalanos JJ, Collier RJ, Romig WR. Enzymic activity of cholera toxin. I. New method of assay and the mechanism of ADP-ribosyl transfer. J Biol Chem. 1979 Jul 10; 254(13):5849-54.
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  251. Mekalanos JJ, Collier RJ, Romig WR. Enzymic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction, and subunit composition. J Biol Chem. 1979 Jul 10; 254(13):5855-61.
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  252. Wisnieski BJ, Shiflett MA, Mekalanos J, Bramhall JS. Analysis of transmembrane dynamics of cholera toxin using photoreactive probes. J Supramol Struct. 1979; 10(2):191-7.
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  253. Mekalanos JJ, Collier RJ, Romig WR. Purification of cholera toxin and its subunits: new methods of preparation and the use of hypertoxinogenic mutants. Infect Immun. 1978 May; 20(2):552-8.
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  254. Mekalanos JJ, Collier RJ, Romig WR. Affinity filters, a new approach to the isolation of tox mutants of Vibrio cholerae. Proc Natl Acad Sci U S A. 1978 Feb; 75(2):941-5.
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  255. Mekalanos JJ, Collier RJ, Romig WR. Simple method for purifying choleragenoid, the natural toxoid of Vibrio cholerae. Infect Immun. 1977 Jun; 16(3):789-95.
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