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Keywords
Last Name
Institution

Hanno Steen, Ph.D.

TitleAssociate Professor of Pathology
InstitutionChildren's Hospital Boston
DepartmentPathology
AddressChildren's Hospital
Pathology/Enders 1161.1
320 Longwood Ave
Boston MA 02115
Phone617/919-2629

 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Paulo JA, Kadiyala V, Gaun A, Sauld JF, Ghoulidi A, Banks PA, Steen H, Conwell DL. Analysis of Endoscopic Pancreatic Function Test (ePFT)-Collected Pancreatic Fluid Proteins Precipitated Via Ultracentrifugation. JOP. 2013; 14(2):176-86.
    View in: PubMed
  2. Berg AH, Drechsler C, Wenger J, Buccafusca R, Hod T, Kalim S, Ramma W, Parikh SM, Steen H, Friedman DJ, Danziger J, Wanner C, Thadhani R, Karumanchi SA. Carbamylation of serum albumin as a risk factor for mortality in patients with kidney failure. Sci Transl Med. 2013 Mar 6; 5(175):175ra29.
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  3. Paulo JA, Gaun A, Kadiyala V, Ghoulidi A, Banks PA, Conwell DL, Steen H. Subcellular fractionation enhances proteome coverage of pancreatic duct cells. Biochim Biophys Acta. 2013 Apr; 1834(4):791-7.
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  4. Serang O, Paulo J, Steen H, Steen JA. A Non-parametric Cutout Index for Robust Evaluation of Identified Proteins. Mol Cell Proteomics. 2013 Mar; 12(3):807-12.
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  5. Kentsis A, Shulman A, Ahmed S, Brennan E, Monuteaux MC, Lee YH, Lipsett S, Paulo JA, Dedeoglu F, Fuhlbrigge R, Bachur R, Bradwin G, Arditi M, Sundel RP, Newburger JW, Steen H, Kim S. Urine proteomics for discovery of improved diagnostic markers of Kawasaki disease. EMBO Mol Med. 2013 Feb; 5(2):210-20.
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  6. Prabakaran S, Lippens G, Steen H, Gunawardena J. Post-translational modification: nature's escape from genetic imprisonment and the basis for dynamic information encoding. Wiley Interdiscip Rev Syst Biol Med. 2012 Nov; 4(6):565-83.
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  7. Huang H, Woo AJ, Waldon Z, Schindler Y, Moran TB, Zhu HH, Feng GS, Steen H, Cantor AB. A Src family kinase-Shp2 axis controls RUNX1 activity in megakaryocyte and T-lymphocyte differentiation. Genes Dev. 2012 Jul 15; 26(14):1587-601.
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  8. Yang W, Kim Y, Kim TK, Keay SK, Kim KP, Steen H, Freeman MR, Hwang D, Kim J. Integration analysis of quantitative proteomics and transcriptomics data identifies potential targets of frizzled-8 protein-related antiproliferative factor in vivo. BJU Int. 2012 Dec; 110(11 Pt C):E1138-46.
    View in: PubMed
  9. Zhang X, Abreu JG, Yokota C, MacDonald BT, Singh S, Coburn KL, Cheong SM, Zhang MM, Ye QZ, Hang HC, Steen H, He X. Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation. Cell. 2012 Jun 22; 149(7):1565-77.
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  10. Paulo JA, Kadiyala V, Banks PA, Steen H, Conwell DL. Mass Spectrometry-Based (GeLC-MS/MS) Comparative Proteomic Analysis of Endoscopically (ePFT) Collected Pancreatic and Gastroduodenal Fluids. Clin Transl Gastroenterol. 2012; 3:e14.
    View in: PubMed
  11. Singh SA, Winter D, Bilimoria PM, Bonni A, Steen H, Steen JA. FLEXIQinase, a mass spectrometry-based assay, to unveil multikinase mechanisms. Nat Methods. 2012 May; 9(5):504-8.
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  12. Renard BY, Xu B, Kirchner M, Zickmann F, Winter D, Korten S, Brattig NW, Tzur A, Hamprecht FA, Steen H. Overcoming species boundaries in peptide identification with Bayesian information criterion-driven error-tolerant peptide search (BICEPS). Mol Cell Proteomics. 2012 Jul; 11(7):M111.014167.
    View in: PubMed
  13. Paulo JA, Kadiyala V, Banks PA, Steen H, Conwell DL. Mass spectrometry-based proteomics for translational research: a technical overview. Yale J Biol Med. 2012 Mar; 85(1):59-73.
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  14. Paulo JA, Lee LS, Banks PA, Steen H, Conwell DL. Proteomic analysis of formalin-fixed paraffin-embedded pancreatic tissue using liquid chromatography tandem mass spectrometry. Pancreas. 2012 Mar; 41(2):175-85.
    View in: PubMed
  15. Paulo JA, Kadiyala V, Lee LS, Banks PA, Conwell DL, Steen H. Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis. J Proteome Res. 2012 Mar 2; 11(3):1897-912.
    View in: PubMed
  16. Kentsis A, Ahmed S, Kurek K, Brennan E, Bradwin G, Steen H, Bachur R. Detection and diagnostic value of urine leucine-rich a-2-glycoprotein in children with suspected acute appendicitis. Ann Emerg Med. 2012 Jul; 60(1):78-83.e1.
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  17. Singh S, Kirchner M, Steen JA, Steen H. A practical guide to the FLEXIQuant method. Methods Mol Biol. 2012; 893:295-319.
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  18. Greenberg SA, Salajegheh M, Judge DP, Feldman MW, Kuncl RW, Waldon Z, Steen H, Wagner KR. Etiology of limb girdle muscular dystrophy 1D/1E determined by laser capture microdissection proteomics. Ann Neurol. 2012 Jan; 71(1):141-5.
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  19. Winter D, Steen H. Optimization of cell lysis and protein digestion protocols for the analysis of HeLa S3 cells by LC-MS/MS. Proteomics. 2011 Dec; 11(24):4726-30.
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  20. Anderson DS, Kirchner M, Kellogg M, Kalish LA, Jeong JY, Vanasse G, Berliner N, Fleming MD, Steen H. Design and validation of a high-throughput matrix-assisted laser desorption ionization time-of-flight mass spectrometry method for quantification of hepcidin in human plasma. Anal Chem. 2011 Nov 1; 83(21):8357-62.
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  21. Broadbelt KG, Rivera KD, Paterson DS, Duncan JR, Trachtenberg FL, Paulo JA, Stapels MD, Borenstein NS, Belliveau RA, Haas EA, Stanley C, Krous HF, Steen H, Kinney HC. Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics. 2012 Jan; 11(1):M111.009530.
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  22. Soblik H, Younis AE, Mitreva M, Renard BY, Kirchner M, Geisinger F, Steen H, Brattig NW. Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases. Mol Cell Proteomics. 2011 Dec; 10(12):M111.010157.
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  23. Wilhelm M, Kirchner M, Steen JA, Steen H. mz5: space- and time-efficient storage of mass spectrometry data sets. Mol Cell Proteomics. 2012 Jan; 11(1):O111.011379.
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  24. Paulo JA, Urrutia R, Banks PA, Conwell DL, Steen H. Proteomic analysis of a rat pancreatic stellate cell line using liquid chromatography tandem mass spectrometry (LC-MS/MS). J Proteomics. 2011 Dec 21; 75(2):708-17.
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  25. Vanasse GJ, Jeong JY, Tate J, Bathulapalli H, Anderson D, Steen H, Fleming M, Mattocks K, Telenti A, Fellay J, Justice AC, Berliner N. A polymorphism in the leptin gene promoter is associated with anemia in patients with HIV disease. Blood. 2011 Nov 17; 118(20):5401-8.
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  26. Paulo JA, Urrutia R, Banks PA, Conwell DL, Steen H. Proteomic analysis of an immortalized mouse pancreatic stellate cell line identifies differentially-expressed proteins in activated vs nonproliferating cell states. J Proteome Res. 2011 Oct 7; 10(10):4835-44.
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  27. Younis AE, Geisinger F, Ajonina-Ekoti I, Soblik H, Steen H, Mitreva M, Erttmann KD, Perbandt M, Liebau E, Brattig NW. Stage-specific excretory-secretory small heat shock proteins from the parasitic nematode Strongyloides ratti--putative links to host's intestinal mucosal defense system. FEBS J. 2011 Sep; 278(18):3319-36.
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  28. Lysaght AC, Kao SY, Paulo JA, Merchant SN, Steen H, Stankovic KM. Proteome of human perilymph. J Proteome Res. 2011 Sep 2; 10(9):3845-51.
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  29. Dowal L, Yang W, Freeman MR, Steen H, Flaumenhaft R. Proteomic analysis of palmitoylated platelet proteins. Blood. 2011 Sep 29; 118(13):e62-73.
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  30. Paulo JA, Lee LS, Banks PA, Steen H, Conwell DL. Difference gel electrophoresis identifies differentially expressed proteins in endoscopically collected pancreatic fluid. Electrophoresis. 2011 Aug; 32(15):1939-51.
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  31. Paulo JA, Lee LS, Wu B, Banks PA, Steen H, Conwell DL. Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay. J Immunol Methods. 2011 Jun 30; 369(1-2):98-107.
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  32. Prabakaran S, Everley RA, Landrieu I, Wieruszeski JM, Lippens G, Steen H, Gunawardena J. Comparative analysis of Erk phosphorylation suggests a mixed strategy for measuring phospho-form distributions. Mol Syst Biol. 2011 Apr 12; 7:482.
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  33. Yang W, Chung YG, Kim Y, Kim TK, Keay SK, Zhang CO, Ji M, Hwang D, Kim KP, Steen H, Freeman MR, Kim J. Quantitative proteomics identifies a beta-catenin network as an element of the signaling response to Frizzled-8 protein-related antiproliferative factor. Mol Cell Proteomics. 2011 Jun; 10(6):M110.007492.
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  34. Paulo JA, Lee LS, Wu B, Banks PA, Steen H, Conwell DL. Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research. Proteomics Clin Appl. 2011 Apr; 5(3-4):109-20.
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  35. Kirchner M, Xu B, Steen H, Steen JA. libfbi: a C++ implementation for fast box intersection and application to sparse mass spectrometry data. Bioinformatics. 2011 Apr 15; 27(8):1166-7.
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  36. Vaezzadeh AR, Briscoe AC, Steen H, Lee RS. One-step sample concentration, purification, and albumin depletion method for urinary proteomics. J Proteome Res. 2010 Nov 5; 9(11):6082-9.
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  37. Armand P, Kim HT, Rhodes J, Sainvil MM, Cutler C, Ho VT, Koreth J, Alyea EP, Hearsey D, Neufeld EJ, Fleming MD, Steen H, Anderson D, Kwong RY, Soiffer RJ, Antin JH. Iron overload in patients with acute leukemia or MDS undergoing myeloablative stem cell transplantation. Biol Blood Marrow Transplant. 2011 Jun; 17(6):852-60.
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  38. Paulo JA, Lee LS, Wu B, Repas K, Banks PA, Conwell DL, Steen H. Proteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS. Proteomics Clin Appl. 2010 Sep; 4(8-9):715-25.
    View in: PubMed
  39. Paulo JA, Lee LS, Wu B, Repas K, Mortele KJ, Banks PA, Steen H, Conwell DL. Identification of pancreas-specific proteins in endoscopically (endoscopic pancreatic function test) collected pancreatic fluid with liquid chromatography--tandem mass spectrometry. Pancreas. 2010 Aug; 39(6):889-96.
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  40. Paulo JA, Lee LS, Wu B, Repas K, Banks PA, Conwell DL, Steen H. Optimized sample preparation of endoscopic collected pancreatic fluid for SDS-PAGE analysis. Electrophoresis. 2010 Jul; 31(14):2377-87.
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  41. Yang W, Cai Q, Lui VW, Everley PA, Kim J, Bhola N, Quesnelle KM, Zetter BR, Steen H, Freeman MR, Grandis JR. Quantitative proteomics analysis reveals molecular networks regulated by epidermal growth factor receptor level in head and neck cancer. J Proteome Res. 2010 Jun 4; 9(6):3073-82.
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  42. Renard BY, Timm W, Kirchner M, Steen JA, Hamprecht FA, Steen H. Estimating the confidence of peptide identifications without decoy databases. Anal Chem. 2010 Jun 1; 82(11):4314-8.
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  43. Timm W, Ozlu N, Steen JJ, Steen H. Effect of high-accuracy precursor masses on phosphopeptide identification from MS3 spectra. Anal Chem. 2010 May 15; 82(10):3977-80.
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  44. Kirchner M, Steen JA, Hamprecht FA, Steen H. MGFp: an open Mascot Generic Format parser library implementation. J Proteome Res. 2010 May 7; 9(5):2762-3.
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  45. Lou X, Kirchner M, Renard BY, Köthe U, Boppel S, Graf C, Lee CT, Steen JA, Steen H, Mayer MP, Hamprecht FA. Deuteration distribution estimation with improved sequence coverage for HX/MS experiments. Bioinformatics. 2010 Jun 15; 26(12):1535-41.
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  46. Ozlu N, Akten B, Timm W, Haseley N, Steen H, Steen JA. Phosphoproteomics. Wiley Interdiscip Rev Syst Biol Med. 2010 May-Jun; 2(3):255-76.
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  47. Prisic S, Dankwa S, Schwartz D, Chou MF, Locasale JW, Kang CM, Bemis G, Church GM, Steen H, Husson RN. Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Proc Natl Acad Sci U S A. 2010 Apr 20; 107(16):7521-6.
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  48. Anderson DS, Heeney MM, Roth U, Menzel C, Fleming MD, Steen H. High-throughput matrix-assisted laser desorption ionization-time-of-flight mass spectrometry method for quantification of hepcidin in human urine. Anal Chem. 2010 Feb 15; 82(4):1551-5.
    View in: PubMed
  49. Kirchner M, Timm W, Fong P, Wangemann P, Steen H. Non-linear classification for on-the-fly fractional mass filtering and targeted precursor fragmentation in mass spectrometry experiments. Bioinformatics. 2010 Mar 15; 26(6):791-7.
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  50. Renard BY, Kirchner M, Monigatti F, Ivanov AR, Rappsilber J, Winter D, Steen JA, Hamprecht FA, Steen H. When less can yield more - Computational preprocessing of MS/MS spectra for peptide identification. Proteomics. 2009 Nov; 9(21):4978-84.
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  51. Kirchner M, Renard BY, Köthe U, Pappin DJ, Hamprecht FA, Steen H, Steen JA. Computational protein profile similarity screening for quantitative mass spectrometry experiments. Bioinformatics. 2010 Jan 1; 26(1):77-83.
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  52. Yang W, Di Vizio D, Kirchner M, Steen H, Freeman MR. Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes. Mol Cell Proteomics. 2010 Jan; 9(1):54-70.
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  53. Ozlü N, Monigatti F, Renard BY, Field CM, Steen H, Mitchison TJ, Steen JJ. Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition. Mol Cell Proteomics. 2010 Feb; 9(2):336-50.
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  54. Tazir Y, Steisslinger V, Soblik H, Younis AE, Beckmann S, Grevelding CG, Steen H, Brattig NW, Erttmann KD. Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti. Mol Biochem Parasitol. 2009 Dec; 168(2):149-57.
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  55. Vaezzadeh AR, Steen H, Freeman MR, Lee RS. Proteomics and opportunities for clinical translation in urological disease. J Urol. 2009 Sep; 182(3):835-43.
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  56. Kentsis A, Lin YY, Kurek K, Calicchio M, Wang YY, Monigatti F, Campagne F, Lee R, Horwitz B, Steen H, Bachur R. Discovery and validation of urine markers of acute pediatric appendicitis using high-accuracy mass spectrometry. Ann Emerg Med. 2010 Jan; 55(1):62-70.e4.
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  57. Huang H, Yu M, Akie TE, Moran TB, Woo AJ, Tu N, Waldon Z, Lin YY, Steen H, Cantor AB. Differentiation-dependent interactions between RUNX-1 and FLI-1 during megakaryocyte development. Mol Cell Biol. 2009 Aug; 29(15):4103-15.
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  58. Jorgensen P, Steen JA, Steen H, Kirschner MW. The mechanism and pattern of yolk consumption provide insight into embryonic nutrition in Xenopus. Development. 2009 May; 136(9):1539-48.
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  59. Singh S, Springer M, Steen J, Kirschner MW, Steen H. FLEXIQuant: a novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J Proteome Res. 2009 May; 8(5):2201-10.
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  60. Wang Z, Dong G, Singh S, Steen H, Li J. A simple and effective method for detecting phosphopeptides for phosphoproteomic analysis. J Proteomics. 2009 Jul 21; 72(5):831-5.
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  61. Kentsis A, Monigatti F, Dorff K, Campagne F, Bachur R, Steen H. Urine proteomics for profiling of human disease using high accuracy mass spectrometry. Proteomics Clin Appl. 2009; 3:1052-61.
  62. Renard BY, Kirchner M, Steen H, Steen JA, Hamprecht FA. NITPICK: peak identification for mass spectrometry data. BMC Bioinformatics. 2008; 9:355.
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  63. Koenig T, Menze BH, Kirchner M, Monigatti F, Parker KC, Patterson T, Steen JJ, Hamprecht FA, Steen H. Robust prediction of the MASCOT score for an improved quality assessment in mass spectrometric proteomics. J Proteome Res. 2008 Sep; 7(9):3708-17.
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  64. Lee RS, Monigatti F, Briscoe AC, Waldon Z, Freeman MR, Steen H. Optimizing sample handling for urinary proteomics. J Proteome Res. 2008 Sep; 7(9):4022-30.
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  65. Adam RM, Yang W, Di Vizio D, Mukhopadhyay NK, Steen H. Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis. BMC Cell Biol. 2008; 9:30.
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  66. Steen JA, Steen H, Georgi A, Parker K, Springer M, Kirchner M, Hamprecht F, Kirschner MW. Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A. 2008 Apr 22; 105(16):6069-74.
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  67. Monigatti F, Steen H. SALAMI (Spectrum ALignments using high Accuracy Mass and hIgh sensitivity data): how to make the best out of hybrid MS/MS data. J Proteome Res. 2008 May; 7(5):1984-93.
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  68. Lee RS, Monigatti F, Lutchman M, Patterson T, Budnik B, Steen JA, Freeman MR, Steen H. Temporal variations of the postnatal rat urinary proteome as a reflection of systemic maturation. Proteomics. 2008 Mar; 8(5):1097-112.
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  69. Yang W, Steen H, Freeman MR. Proteomic approaches to the analysis of multiprotein signaling complexes. Proteomics. 2008 Feb; 8(4):832-51.
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  70. Bensadek D, Monigatti F, Steen JAJ, Steen H. Why b, y’s? Sodiation-induced tryptic peptide-like fragmentation of non-tryptic peptides. Int J Mass Spectrom. 2007; 268:181-189.
  71. Kirchner M, Saussen B, Steen H, Steen JAJ, Hamprecht FA. amsrpm: Robust Point Matching for Retention Time Alignment of LC/MS Data with R. J Stat Software. 2007; 18:4.
  72. Monigatti F, Hekking B, Steen H. Protein sulfation analysis--A primer. Biochim Biophys Acta. 2006 Dec; 1764(12):1904-13.
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  73. MacLellan DL, Steen H, Adam RM, Garlick M, Zurakowski D, Gygi SP, Freeman MR, Solomon KR. A quantitative proteomic analysis of growth factor-induced compositional changes in lipid rafts of human smooth muscle cells. Proteomics. 2005 Dec; 5(18):4733-42.
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  74. Steen H, Jebanathirajah JA, Rush J, Morrice N, Kirschner MW. Phosphorylation analysis by mass spectrometry: myths, facts, and the consequences for qualitative and quantitative measurements. Mol Cell Proteomics. 2006 Jan; 5(1):172-81.
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  75. Steen H, Jebanathirajah JA, Springer M, Kirschner MW. Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc Natl Acad Sci U S A. 2005 Mar 15; 102(11):3948-53.
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  76. Auty R, Steen H, Myers LC, Persinger J, Bartholomew B, Gygi SP, Buratowski S. Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem. 2004 Nov 26; 279(48):49973-81.
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  77. Steen H, Mann M. The ABC's (and XYZ's) of peptide sequencing. Nat Rev Mol Cell Biol. 2004 Sep; 5(9):699-711.
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  78. Argetsinger LS, Kouadio JL, Steen H, Stensballe A, Jensen ON, Carter-Su C. Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity. Mol Cell Biol. 2004 Jun; 24(11):4955-67.
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  79. Gautreau A, Ho HY, Li J, Steen H, Gygi SP, Kirschner MW. Purification and architecture of the ubiquitous Wave complex. Proc Natl Acad Sci U S A. 2004 Mar 30; 101(13):4379-83.
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  80. Peri S, Navarro JD, Amanchy R, Kristiansen TZ, Jonnalagadda CK, Surendranath V, Niranjan V, Muthusamy B, Gandhi TK, Gronborg M, Ibarrola N, Deshpande N, Shanker K, Shivashankar HN, Rashmi BP, Ramya MA, Zhao Z, Chandrika KN, Padma N, Harsha HC, Yatish AJ, Kavitha MP, Menezes M, Choudhury DR, Suresh S, Ghosh N, Saravana R, Chandran S, Krishna S, Joy M, Anand SK, Madavan V, Joseph A, Wong GW, Schiemann WP, Constantinescu SN, Huang L, Khosravi-Far R, Steen H, Tewari M, Ghaffari S, Blobe GC, Dang CV, Garcia JG, Pevsner J, Jensen ON, Roepstorff P, Deshpande KS, Chinnaiyan AM, Hamosh A, Chakravarti A, Pandey A. Development of human protein reference database as an initial platform for approaching systems biology in humans. Genome Res. 2003 Oct; 13(10):2363-71.
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  81. Li J, Steen H, Gygi SP. Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents: the yeast salinity stress response. Mol Cell Proteomics. 2003 Nov; 2(11):1198-204.
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  82. Jebanathirajah J, Steen H, Roepstorff P. Using optimized collision energies and high resolution, high accuracy fragment ion selection to improve glycopeptide detection by precursor ion scanning. J Am Soc Mass Spectrom. 2003 Jul; 14(7):777-84.
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  83. Steen H, Fernandez M, Ghaffari S, Pandey A, Mann M. Phosphotyrosine mapping in Bcr/Abl oncoprotein using phosphotyrosine-specific immonium ion scanning. Mol Cell Proteomics. 2003 Mar; 2(3):138-45.
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  84. Steen H, Mann M. Analysis of bromotryptophan and hydroxyproline modifications by high-resolution, high-accuracy precursor ion scanning utilizing fragment ions with mass-deficient mass tags. Anal Chem. 2002 Dec 15; 74(24):6230-6.
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  85. Steen H, Pandey A, Andersen JS, Mann M. Analysis of tyrosine phosphorylation sites in signaling molecules by a phosphotyrosine-specific immonium ion scanning method. Sci STKE. 2002 Oct 15; 2002(154):pl16.
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  86. Steen H, Pandey A. Proteomics goes quantitative: measuring protein abundance. Trends Biotechnol. 2002 Sep; 20(9):361-4.
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  87. Steen H, Mann M. A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode. J Am Soc Mass Spectrom. 2002 Aug; 13(8):996-1003.
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  88. Mann M, Ong SE, Grønborg M, Steen H, Jensen ON, Pandey A. Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol. 2002 Jun; 20(6):261-8.
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  89. Steen H, Jensen ON. Analysis of protein-nucleic acid interactions by photochemical cross-linking and mass spectrometry. Mass Spectrom Rev. 2002 May-Jun; 21(3):163-82.
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  90. Ong SE, Blagoev B, Kratchmarova I, Kristensen DB, Steen H, Pandey A, Mann M. Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol Cell Proteomics. 2002 May; 1(5):376-86.
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  91. Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI. Directed proteomic analysis of the human nucleolus. Curr Biol. 2002 Jan 8; 12(1):1-11.
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  92. Peri S, Steen H, Pandey A. GPMAW--a software tool for analyzing proteins and peptides. Trends Biochem Sci. 2001 Nov; 26(11):687-9.
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  93. Steen H, Kuster B, Fernandez M, Pandey A, Mann M. Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway. J Biol Chem. 2002 Jan 11; 277(2):1031-9.
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  94. Steen H, Petersen J, Mann M, Jensen ON. Mass spectrometric analysis of a UV-cross-linked protein-DNA complex: tryptophans 54 and 88 of E. coli SSB cross-link to DNA. Protein Sci. 2001 Oct; 10(10):1989-2001.
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  95. Steen H, Küster B, Mann M. Quadrupole time-of-flight versus triple-quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanning. J Mass Spectrom. 2001 Jul; 36(7):782-90.
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  96. Rappsilber J, Steen H, Mann M. Labile sulfogroup allows differentiation of sulfotyrosine and phosphotyrosine in peptides. J Mass Spectrom. 2001 Jul; 36(7):832-3.
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  97. Petersson AS, Steen H, Kalume DE, Caidahl K, Roepstorff P. Investigation of tyrosine nitration in proteins by mass spectrometry. J Mass Spectrom. 2001 Jun; 36(6):616-25.
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  98. Steen H, Küster B, Fernandez M, Pandey A, Mann M. Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Anal Chem. 2001 Apr 1; 73(7):1440-8.
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  99. Spada F, Steen H, Troedsson C, Kallesoe T, Spriet E, Mann M, Thompson EM. Molecular patterning of the oikoplastic epithelium of the larvacean tunicate Oikopleura dioica. J Biol Chem. 2001 Jun 8; 276(23):20624-32.
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  100. Steen H, Mann M. Similarity between condensed phase and gas phase chemistry: fragmentation of peptides containing oxidized cysteine residues and its implications for proteomics. J Am Soc Mass Spectrom. 2001 Feb; 12(2):228-32.
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  101. Steen, H. Mass Spectrometry for the Analysis of Protein Modifications (Ph.D. Thesis). 2001.
  102. Pandey A, Fernandez MM, Steen H, Blagoev B, Nielsen MM, Roche S, Mann M, Lodish HF. Identification of a novel immunoreceptor tyrosine-based activation motif-containing molecule, STAM2, by mass spectrometry and its involvement in growth factor and cytokine receptor signaling pathways. J Biol Chem. 2000 Dec 8; 275(49):38633-9.
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  103. Dahle J, Mikalsen SO, Rivedal E, Steen HB. Gap junctional intercellular communication is not a major mediator in the bystander effect in photodynamic treatment of MDCK II cells. Radiat Res. 2000 Sep; 154(3):331-41.
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  104. Gobom J, Kraeuter KO, Persson R, Steen H, Roepstorff P, Ekman R. Detection and quantification of neurotensin in human brain tissue by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2000 Jul 15; 72(14):3320-6.
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  105. Studer A, Steen H. The SHi Reaction at Silicon – A New Entry into Cyclic Alkoxy-silanes. Chem Eur J. 1999; 5:759-73.
  106. Summerfield SG, Steen H, O’Malley M, Gaskell SJ. Phenyl thiocarbamoyl and related derivatives of peptides: Edman chemistry in the gas phase. Int J Mass Spectrom. 1999; 188:95-103.
  107. Steen, H. Intramolecular Homolytic Substitution on Silicon – Influence of the Degree of Substitution on the Rate of Cyclisation (Dipl. Chem. Thesis). 1998.
  108. Studer A, Bossart M, Steen H. Stereoselective Phenyl Migration from Silicon to Carbon. Tetrahedron Lett. 1998; 39:8829-32.
  109. Steen, H. Techniques for the Mass Spectrometric Localisation of Double Bonds (B.Sc. Thesis). 1995.
  110. Stokke T, Holte H, Smeland EB, Lie SO, Steen HB. Differential chromatin structure-dependent binding of 7-aminoactinomycin D in normal and malignant bone marrow hematopoietic cells. Cancer Res. 1992 Sep 15; 52(18):5007-12.
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  111. Berg K, Steen HB, Winkelman JW, Moan J. Synergistic effects of photoactivated tetra(4-sulfonatophenyl)porphine and nocodazole on microtubule assembly, accumulation of cells in mitosis and cell survival. J Photochem Photobiol B. 1992 Apr 15; 13(1):59-70.
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  112. Stokke T, Erikstein B, Holte H, Funderud S, Steen HB. Cell cycle-specific expression and nuclear binding of DNA polymerase alpha. Mol Cell Biol. 1991 Jun; 11(6):3384-9.
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  113. Steen HB. Further developments of a microscope-based flow cytometer: light scatter detection and excitation intensity compensation. Cytometry. 1980 Jul; 1(1):26-31.
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  114. Steen HB. Molecular excitations in radiation damage as studies by the radiation-induced luminescence of proteins. Scand J Clin Lab Invest Suppl. 1968; 106:21-30.
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