Lynn Bry, PH.D., M.D.
|Title||Assistant Professor of Pathology|
|Institution||Brigham and Women's Hospital|
|Address||Brigham and Womens Hospital|
Dept. Pathology, EBRC, 411
221 Longwood Ave
Boston MA 02115
Research: Mucosal Immunology/Infectious Disease
My laboratory studies the following aspects of host defense against microbial pathogens (1) host reponses against attaching and effacing pathogens such as Citrobacter rodentium and the enteropathogenic E. coli (EPEC), (2) the molecular basis of immunoprotection from colonization with commensals and (3) recruitment of adaptive memory responses against non-invasive enteric pathogens to fight primary infection with phylogenetically related invasive species.
We have defined the effector functions and locations at which protective T cell and B cell responses develop against the murine attaching and effacing pathogen Citrobacter rodentium. This EPEC-like organism produces a primary infection of the apical surface of the gut epithelium. Surprisingly, systemic and CD4+ T cell dependent IgG, and not mucosal IgA, proved the critical adaptive response needed to successfully control mucosal infection. Pathogen-specific IgG acts systemically and in the gut lumen to effect erradication of pathogens from the host. We have subsequently shown that interactions with complement are important for this erradication and for the development of specific IgG.
We have further developed this model to elucidate how the commensal flora primes adaptive responses recruited early in infection with C. rodentium. By forming the HDDC Gnotobiotic Mouse Core, we expect to be able to define how members of the commensal flora provide protection against phylogenetically related or unrelated organisms, through mechanisms including colonization resistance as well as cognate and non-cognate effects upon the immune system.
Research: Crimson Application - IT infrastructure to support high-through sample collection and downstream applications for a research enterprise.
Crimson prospectively matches discarded samples from clinical laboratories per investigator-defined criteria. This feature effectively harnesses the thousands of samples and associated data generated daily in clinical laboratories for productive use in IRB-approved studies. Additional functions of Crimson include management of sample inventories, user workgroups, and auditing capabilities to insure compliance with HIPAA and local IRBs. As of May 2007, Crimson has been integrated with the Research Patient Data Registry (RPDR), a clinical data repository that houses electronic information generated at BWH and MGH. This integration allows an investigator to define a cohort of patients on the RPDR and forward it to Crimson for collection of discarded samples.
Crimson went live on 2/1/2007 and has since handled >15,000 samples in its first year of operations, including >10,000 clinical trials samples and >5,000 discarded pathology samples collected for use by IRB-approved studies. The application was developed by myself and Neil Herring in the Pathology IT Division at BWH with software development support for Daedalus Software Inc. in Cambridge MA. Crimson remains a unique an innovative system, one that is being adopted by i2b2 (Informatics for Integrating Biology to the Bedside), an NIH Roadmap initiative.
I teach in the medical school courses IN704.0: "Immunology, Microbiology and Infectious Disease," HST-040: "Mechanisms of Microbial Pathogenesis" and the clerkship PA511M.J: "Clinical Laboratory Medicine." I am also course director for the clinical microbiology rotation at Brigham & Women's Hospital through which pathology residents and infectious disease fellows rotate, and organize the weekly plate rounds between the Clinical Laboratories and Division of Infectious Disease at Brigham & Women’s Hospital.
I am also a lecturer and mentor for the Project Success Program at Harvard Medical School.
As Associate Medical Director in the Clinical Microbiology Laboratory at Brigham and Women's Hospital, my duties include oversight of routine microbiological testing, evaluation and implementation of new modalities for diagnosing infectious diseases and determining antimicrobial resistance.
Since 2006 I have successfully worked to bring in additional high-throughput platforms for the molecular diagnosis of infectious agents, including HIV, HCV, gonorrhea and Chlamydia. I have also worked with clinicians in the renal division to implement decision/support trees based on key data from testing for BK virus in renal transplant patients, and with individuals in the pulmonary division to assist with microbiological assessment of cystic fibrosis patients seen at BWH.
Available: 10/21/09, Expires: 06/30/14
We are evaluating how diversity of the gut commensal flora impacts the host's susceptibility to different enteric pathogens. Available Jan 1, 2010.
The position will interact with other lab staff to characterize commensal species shown to impact host susceptibility to different infectious agents. Studies involve evaluation of the gut microbiome by 16S rRNA deep sequencing and in vivo analyses of germfree mice colonized with specific commensals to demonstrate effects on ability of different pathogens to colonize and infect the host.
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