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Ann Hochschild, Ph.D.

TitleMaude and Lillian Presley Professor of Microbiology and Immunobiology
InstitutionHarvard Medical School
DepartmentMicrobiology and Immunobiology
AddressHarvard Inst of Medicine
Microbiology and Immunobiology, Room 1027
4 Blackfan Cir
Boston MA 02115
Phone617/432-1986
Fax617/738-7664

 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Sivanathan V, Hochschild A. A bacterial export system for generating extracellular amyloid aggregates. Nat Protoc. 2013 Jun 20; 8(7):1381-90.
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  2. Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Aug; 41(15):7429-37.
    View in: PubMed
  3. Montero-Diez C, Deighan P, Osmundson J, Darst SA, Hochschild A. Phage-Encoded Inhibitor of Staphylococcus aureus Transcription Exerts Context-Dependent Effects on Promoter Function in a Modified Escherichia coli-Based Transcription System. J Bacteriol. 2013 Aug; 195(16):3621-8.
    View in: PubMed
  4. Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA. Promoter-Specific Transcription Inhibition in Staphylococcus aureus by a Phage Protein. Cell. 2012 Nov 21; 151(5):1005-16.
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  5. Sivanathan V, Hochschild A. Generating extracellular amyloid aggregates using E. coli cells. Genes Dev. 2012 Dec 1; 26(23):2659-67.
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  6. Turner A, Hochschild A, Burnett J, Zulfiqar A, Dyer CB. High prevalence of medication non-adherence in a sample of community-dwelling older adults with adult protective services-validated self-neglect. Drugs Aging. 2012 Sep; 29(9):741-9.
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  7. Fixen KR, Janakiraman A, Garrity S, Slade DJ, Gray AN, Karahan N, Hochschild A, Goldberg MB. Genetic reporter system for positioning of proteins at the bacterial pole. MBio. 2012; 3(2).
    View in: PubMed
  8. Twist KA, Campbell EA, Deighan P, Nechaev S, Jain V, Geiduschek EP, Hochschild A, Darst SA. Crystal structure of the bacteriophage T4 late-transcription coactivator gp33 with the ß-subunit flap domain of Escherichia coli RNA polymerase. Proc Natl Acad Sci U S A. 2011 Dec 13; 108(50):19961-6.
    View in: PubMed
  9. Deighan P, Pukhrambam C, Nickels BE, Hochschild A. Initial transcribed region sequences influence the composition and functional properties of the bacterial elongation complex. Genes Dev. 2011 Jan 1; 25(1):77-88.
    View in: PubMed
  10. Garrity SJ, Sivanathan V, Dong J, Lindquist S, Hochschild A. Conversion of a yeast prion protein to an infectious form in bacteria. Proc Natl Acad Sci U S A. 2010 Jun 8; 107(23):10596-601.
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  11. Yuan AH, Hochschild A. Direct activator/co-activator interaction is essential for bacteriophage T4 middle gene expression. Mol Microbiol. 2009 Nov; 74(4):1018-30.
    View in: PubMed
  12. Rao X, Deighan P, Hua Z, Hu X, Wang J, Luo M, Wang J, Liang Y, Zhong G, Hochschild A, Shen L. A regulator from Chlamydia trachomatis modulates the activity of RNA polymerase through direct interaction with the beta subunit and the primary sigma subunit. Genes Dev. 2009 Aug 1; 23(15):1818-29.
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  13. Stallings CL, Stephanou NC, Chu L, Hochschild A, Nickels BE, Glickman MS. CarD is an essential regulator of rRNA transcription required for Mycobacterium tuberculosis persistence. Cell. 2009 Jul 10; 138(1):146-59.
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  14. Yuan AH, Nickels BE, Hochschild A. The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase. Proc Natl Acad Sci U S A. 2009 Apr 21; 106(16):6597-602.
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  15. Hochschild A, Lewis M. The bacteriophage lambda CI protein finds an asymmetric solution. Curr Opin Struct Biol. 2009 Feb; 19(1):79-86.
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  16. Deighan P, Diez CM, Leibman M, Hochschild A, Nickels BE. The bacteriophage lambda Q antiterminator protein contacts the beta-flap domain of RNA polymerase. Proc Natl Acad Sci U S A. 2008 Oct 7; 105(40):15305-10.
    View in: PubMed
  17. Yuan AH, Gregory BD, Sharp JS, McCleary KD, Dove SL, Hochschild A. Rsd family proteins make simultaneous interactions with regions 2 and 4 of the primary sigma factor. Mol Microbiol. 2008 Dec; 70(5):1136-51.
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  18. Stayrook S, Jaru-Ampornpan P, Ni J, Hochschild A, Lewis M. Crystal structure of the lambda repressor and a model for pairwise cooperative operator binding. Nature. 2008 Apr 24; 452(7190):1022-5.
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  19. Hochschild A. Gene-specific regulation by a transcript cleavage factor: facilitating promoter escape. J Bacteriol. 2007 Dec; 189(24):8769-71.
    View in: PubMed
  20. Leibman M, Hochschild A. A sigma-core interaction of the RNA polymerase holoenzyme that enhances promoter escape. EMBO J. 2007 Mar 21; 26(6):1579-90.
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  21. Deighan P, Hochschild A. The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Mol Microbiol. 2007 Feb; 63(3):911-20.
    View in: PubMed
  22. Nickels BE, Roberts CW, Roberts JW, Hochschild A. RNA-mediated destabilization of the sigma(70) region 4/beta flap interaction facilitates engagement of RNA polymerase by the Q antiterminator. Mol Cell. 2006 Nov 3; 24(3):457-68.
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  23. Deighan P, Hochschild A. Conformational toggle triggers a modulator of RNA polymerase activity. Trends Biochem Sci. 2006 Aug; 31(8):424-6.
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  24. Pinkett HW, Shearwin KE, Stayrook S, Dodd IB, Burr T, Hochschild A, Egan JB, Lewis M. The structural basis of cooperative regulation at an alternate genetic switch. Mol Cell. 2006 Mar 3; 21(5):605-15.
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  25. Deaconescu AM, Chambers AL, Smith AJ, Nickels BE, Hochschild A, Savery NJ, Darst SA. Structural basis for bacterial transcription-coupled DNA repair. Cell. 2006 Feb 10; 124(3):507-20.
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  26. Gregory BD, Deighan P, Hochschild A. An artificial activator that contacts a normally occluded surface of the RNA polymerase holoenzyme. J Mol Biol. 2005 Oct 28; 353(3):497-506.
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  27. Gregory BD, Nickels BE, Darst SA, Hochschild A. An altered-specificity DNA-binding mutant of Escherichia coli sigma70 facilitates the analysis of sigma70 function in vivo. Mol Microbiol. 2005 Jun; 56(5):1208-19.
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  28. Nickels BE, Garrity SJ, Mekler V, Minakhin L, Severinov K, Ebright RH, Hochschild A. The interaction between sigma70 and the beta-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation. Proc Natl Acad Sci U S A. 2005 Mar 22; 102(12):4488-93.
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  29. Altman S, Bassler BL, Beckwith J, Belfort M, Berg HC, Bloom B, Brenchley JE, Campbell A, Collier RJ, Connell N, Cozzarelli NR, Craig NL, Darst S, Ebright RH, Elledge SJ, Falkow S, Galan JE, Gottesman M, Gourse R, Grindley ND, Gross CA, Grossman A, Hochschild A, Howe M, Hurwitz J, Isberg RR, Kaplan S, Kornberg A, Kustu SG, Landick RC, Landy A, Levy SB, Losick R, Long SR, Maloy SR, Mekalanos JJ, Neidhardt FC, Pace NR, Ptashne M, Roberts JW, Roth JR, Rothman-Denes LB, Salyers A, Schaechter M, Shapiro L, Silhavy TJ, Simon MI, Walker G, Yanofsky C, Zinder N. An open letter to Elias Zerhouni. Science. 2005 Mar 4; 307(5714):1409-10.
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  30. Pineda M, Gregory BD, Szczypinski B, Baxter KR, Hochschild A, Miller ES, Hinton DM. A family of anti-sigma70 proteins in T4-type phages and bacteria that are similar to AsiA, a Transcription inhibitor and co-activator of bacteriophage T4. J Mol Biol. 2004 Dec 10; 344(5):1183-97.
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  31. Sun L, Dove SL, Panaghie G, deHaseth PL, Hochschild A. An RNA polymerase mutant deficient in DNA melting facilitates study of activation mechanism: application to an artificial activator of transcription. J Mol Biol. 2004 Nov 5; 343(5):1171-82.
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  32. Harnasch M, Grau S, Behrends C, Dove SL, Hochschild A, Iskandar MK, Xia W, Ehrmann M. Characterization of presenilin-amyloid precursor interaction using bacterial expression and two-hybrid systems for human membrane proteins. Mol Membr Biol. 2004 Nov-Dec; 21(6):373-83.
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  33. Nickels BE, Hochschild A. Regulation of RNA polymerase through the secondary channel. Cell. 2004 Aug 6; 118(3):281-4.
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  34. Nickels BE, Mukhopadhyay J, Garrity SJ, Ebright RH, Hochschild A. The sigma 70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter. Nat Struct Mol Biol. 2004 Jun; 11(6):544-50.
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  35. Gregory BD, Nickels BE, Garrity SJ, Severinova E, Minakhin L, Urbauer RJ, Urbauer JL, Heyduk T, Severinov K, Hochschild A. A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme. Proc Natl Acad Sci U S A. 2004 Mar 30; 101(13):4554-9.
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  36. Dodd IB, Shearwin KE, Perkins AJ, Burr T, Hochschild A, Egan JB. Cooperativity in long-range gene regulation by the lambda CI repressor. Genes Dev. 2004 Feb 1; 18(3):344-54.
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  37. Jain D, Nickels BE, Sun L, Hochschild A, Darst SA. Structure of a ternary transcription activation complex. Mol Cell. 2004 Jan 16; 13(1):45-53.
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  38. Dove SL, Hochschild A. A bacterial two-hybrid system based on transcription activation. Methods Mol Biol. 2004; 261:231-46.
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  39. Dove SL, Darst SA, Hochschild A. Region 4 of sigma as a target for transcription regulation. Mol Microbiol. 2003 May; 48(4):863-74.
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  40. Nickels BE, Dove SL, Murakami KS, Darst SA, Hochschild A. Protein-protein and protein-DNA interactions of sigma70 region 4 involved in transcription activation by lambdacI. J Mol Biol. 2002 Nov 15; 324(1):17-34.
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  41. Nickels BE, Roberts CW, Sun H, Roberts JW, Hochschild A. The sigma(70) subunit of RNA polymerase is contacted by the (lambda)Q antiterminator during early elongation. Mol Cell. 2002 Sep; 10(3):611-22.
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  42. Shaywitz AJ, Dove SL, Greenberg ME, Hochschild A. Analysis of phosphorylation-dependent protein-protein interactions using a bacterial two-hybrid system. Sci STKE. 2002 Jul 23; 2002(142):pl11.
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  43. Pande S, Makela A, Dove SL, Nickels BE, Hochschild A, Hinton DM. The bacteriophage T4 transcription activator MotA interacts with the far-C-terminal region of the sigma70 subunit of Escherichia coli RNA polymerase. J Bacteriol. 2002 Jul; 184(14):3957-64.
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  44. Hochschild A. The lambda switch: cI closes the gap in autoregulation. Curr Biol. 2002 Feb 5; 12(3):R87-9.
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  45. Kuznedelov K, Minakhin L, Niedziela-Majka A, Dove SL, Rogulja D, Nickels BE, Hochschild A, Heyduk T, Severinov K. A role for interaction of the RNA polymerase flap domain with the sigma subunit in promoter recognition. Science. 2002 Feb 1; 295(5556):855-7.
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  46. Dove SL, Hochschild A. Bacterial two-hybrid analysis of interactions between region 4 of the sigma(70) subunit of RNA polymerase and the transcriptional regulators Rsd from Escherichia coli and AlgQ from Pseudomonas aeruginosa. J Bacteriol. 2001 Nov; 183(21):6413-21.
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  47. Langdon RC, Burr T, Pagan-Westphal S, Hochschild A. A chimeric activator of transcription that uses two DNA-binding domains to make simultaneous contact with pairs of recognition sites. Mol Microbiol. 2001 Aug; 41(4):885-96.
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  48. Shaywitz AJ, Dove SL, Kornhauser JM, Hochschild A, Greenberg ME. Magnitude of the CREB-dependent transcriptional response is determined by the strength of the interaction between the kinase-inducible domain of CREB and the KIX domain of CREB-binding protein. Mol Cell Biol. 2000 Dec; 20(24):9409-22.
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  49. Dove SL, Huang FW, Hochschild A. Mechanism for a transcriptional activator that works at the isomerization step. Proc Natl Acad Sci U S A. 2000 Nov 21; 97(24):13215-20.
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  50. Bell CE, Frescura P, Hochschild A, Lewis M. Crystal structure of the lambda repressor C-terminal domain provides a model for cooperative operator binding. Cell. 2000 Jun 23; 101(7):801-11.
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  51. Blum JH, Dove SL, Hochschild A, Mekalanos JJ. Isolation of peptide aptamers that inhibit intracellular processes. Proc Natl Acad Sci U S A. 2000 Feb 29; 97(5):2241-6.
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  52. Hu JC, Kornacker MG, Hochschild A. Escherichia coli one- and two-hybrid systems for the analysis and identification of protein-protein interactions. Methods. 2000 Jan; 20(1):80-94.
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  53. Podolny V, Lin EC, Hochschild A. A cyclic AMP receptor protein mutant that constitutively activates an Escherichia coli promoter disrupted by an IS5 insertion. J Bacteriol. 1999 Dec; 181(24):7457-63.
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  54. Langdon RC, Hochschild A. A genetic method for dissecting the mechanism of transcriptional activator synergy by identical activators. Proc Natl Acad Sci U S A. 1999 Oct 26; 96(22):12673-8.
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  55. Whipple FW, Hou EF, Hochschild A. Amino acid-amino acid contacts at the cooperativity interface of the bacteriophage lambda and P22 repressors. Genes Dev. 1998 Sep 1; 12(17):2791-802.
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  56. Hochschild A, Dove SL. Protein-protein contacts that activate and repress prokaryotic transcription. Cell. 1998 Mar 6; 92(5):597-600.
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  57. Dove SL, Hochschild A. Conversion of the omega subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target. Genes Dev. 1998 Mar 1; 12(5):745-54.
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  58. Dove SL, Hochschild A. Use of artificial activators to define a role for protein-protein and protein-DNA contacts in transcriptional activation. Cold Spring Harb Symp Quant Biol. 1998; 63:173-80.
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  59. Dove SL, Joung JK, Hochschild A. Activation of prokaryotic transcription through arbitrary protein-protein contacts. Nature. 1997 Apr 10; 386(6625):627-30.
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  60. Whipple FW, Ptashne M, Hochschild A. The activation defect of a lambda cI positive control mutant. J Mol Biol. 1997 Jan 24; 265(3):261-5.
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  61. Joung JK, Chung EH, King G, Yu C, Hirsh AS, Hochschild A. Genetic strategy for analyzing specificity of dimer formation: Escherichia coli cyclic AMP receptor protein mutant altered in its dimerization specificity. Genes Dev. 1995 Dec 1; 9(23):2986-96.
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  62. Joung JK, Koepp DM, Hochschild A. Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein. Science. 1994 Sep 23; 265(5180):1863-6.
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  63. Whipple FW, Kuldell NH, Cheatham LA, Hochschild A. Specificity determinants for the interaction of lambda repressor and P22 repressor dimers. Genes Dev. 1994 May 15; 8(10):1212-23.
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  64. Kuldell N, Hochschild A. Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. J Bacteriol. 1994 May; 176(10):2991-8.
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  65. Hochschild A. Transcriptional activation. How lambda repressor talks to RNA polymerase. Curr Biol. 1994 May 1; 4(5):440-2.
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  66. Joung JK, Le LU, Hochschild A. Synergistic activation of transcription by Escherichia coli cAMP receptor protein. Proc Natl Acad Sci U S A. 1993 Apr 1; 90(7):3083-7.
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  67. Hochschild A. Detecting cooperative protein-DNA interactions and DNA loop formation by footprinting. Methods Enzymol. 1991; 208:343-61.
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  68. Hochschild A, Ptashne M. Interaction at a distance between lambda repressors disrupts gene activation. Nature. 1988 Nov 24; 336(6197):353-7.
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  69. Hochschild A, Douhan J, Ptashne M. How lambda repressor and lambda Cro distinguish between OR1 and OR3. Cell. 1986 Dec 5; 47(5):807-16.
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  70. Griffith J, Hochschild A, Ptashne M. DNA loops induced by cooperative binding of lambda repressor. Nature. 1986 Aug 21-27; 322(6081):750-2.
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  71. Hochschild A, Ptashne M. Homologous interactions of lambda repressor and lambda Cro with the lambda operator. Cell. 1986 Mar 28; 44(6):925-33.
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  72. Hochschild A, Ptashne M. Cooperative binding of lambda repressors to sites separated by integral turns of the DNA helix. Cell. 1986 Mar 14; 44(5):681-7.
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  73. Hochschild A, Irwin N, Ptashne M. Repressor structure and the mechanism of positive control. Cell. 1983 Feb; 32(2):319-25.
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  74. Guarente L, Nye JS, Hochschild A, Ptashne M. Mutant lambda phage repressor with a specific defect in its positive control function. Proc Natl Acad Sci U S A. 1982 Apr; 79(7):2236-9.
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  75. Hochschild AR. A review of sex role research. AJS. 1973 Jan; 78(4):1011-29.
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