Contact, publication, and social network information about Harvard faculty and fellows. Harvard Catalyst Profiles
Keywords
Last Name
Institution

Douglas B. Cowan, Ph.D.

TitleAssociate Professor of Anaesthesia
InstitutionChildren's Hospital Boston
DepartmentAnaesthesia
AddressChildrens Hospital
Anesthesiology (Enders 320)
300 Longwood Ave
Boston MA 02115
Phone617/919-2655

 Overview 
 overview
Our laboratory studies the cardiovascular system with an emphasis on combining tissue engineering and stem cell biology to improve heart function.

Current projects in our laboratory include the following:

Cardiac Conduction through Engineered Tissue – We are engineering tissues to act as electrical conduits for use in pediatric patients with complete heart block. Ultimately, we hope to provide a realistic alternative or accessory treatment to conventional cardiac pacemaker implantation in children.

Adult Stem Cell Culture – We are developing methods to expand and control the fate of tissue-specific stem cells to determine their regenerative potential for the treatment of cardiac and skeletal muscle disorders. Our studies are designed to advance cell-based therapies for a variety of human diseases.

Mitochondrial Transplantation – This collaborative project is focused on providing a clinically-relevant treatment for myocardial infarction in adults. Accordingly, we and our colleagues are injecting autologous mitochondria into the heart to decrease the extent of damage and enhance functional recovery.


Specialties:
Molecular Biology, Cell Biology, Cardiac Physiology, Biochemistry, Electrophysiology, Stem Cell Biology, Confocal Microscopy, Fluorescence Microscopy, Electron Microscopy, Protein Chemistry, Cell Culture, Bioengineering, Optical Surface Mapping, Cardiovascular Surgery, Tissue Engineering.


 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Bartholomä MD, He H, Pacak CA, Dunning P, Fahey FH, McGowan FX, Cowan DB, Treves ST, Packard AB. Biological characterization of F-18-labeled rhodamine B, a potential positron emission tomography perfusion tracer. Nucl Med Biol. 2013 Nov; 40(8):1043-8.
    View in: PubMed
  2. Pacak CA, Eddy MT, Woodhull L, Wang KR, Alpatov I, Fullen S, Dowd RP, Choi YH, Cowan DB. Microcarrier-based expansion of adult murine side population stem cells. PLoS One. 2013; 8(1):e55187.
    View in: PubMed
  3. Masuzawa A, Black KM, Pacak CA, Ericsson M, Barnett RJ, Drumm C, Seth P, Bloch DB, Levitsky S, Cowan DB, McCully JD. Transplantation of autologously derived mitochondria protects the heart from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2013 Apr; 304(7):H966-82.
    View in: PubMed
  4. Friehs I, Cowan DB, Choi YH, Black KM, Barnett R, Bhasin MK, Daly C, Dillon SJ, Libermann TA, McGowan FX, Del Nido PJ, Levitsky S, McCully JD. Pressure-overload hypertrophy of the developing heart reveals activation of divergent gene and protein pathways in the left and right ventricular myocardium. Am J Physiol Heart Circ Physiol. 2013 Mar; 304(5):H697-708.
    View in: PubMed
  5. Neef K, Choi YH, Perumal Srinivasan S, Treskes P, Cowan DB, Stamm C, Rubach M, Adelmann R, Wittwer T, Wahlers T. Mechanical preconditioning enables electrophysiologic coupling of skeletal myoblast cells to myocardium. J Thorac Cardiovasc Surg. 2012 Nov; 144(5):1176-1184.e1.
    View in: PubMed
  6. Perumal Srinivasan S, Neef K, Treskes P, Liakopoulos OJ, Stamm C, Cowan DB, Madershahian N, Kuhn E, Slottosch I, Wittwer T, Wahlers T, Choi YH. Enhanced gap junction expression in myoblast-containing engineered tissue. Biochem Biophys Res Commun. 2012 Jun 8; 422(3):462-8.
    View in: PubMed
  7. Pacak CA, Powers JM, Cowan DB. Ultrarapid purification of collagen type I for tissue engineering applications. Tissue Eng Part C Methods. 2011 Sep; 17(9):879-85.
    View in: PubMed
  8. Sill B, Roy N, Hammer PE, Triedman JK, Sigg DC, Kelly MF, Nedder A, Dunning PS, Cowan DB. Development of an ovine model of pediatric complete heart block. J Surg Res. 2011 Apr; 166(2):e103-8.
    View in: PubMed
  9. Wolf CM, Seslar SP, den Boer K, Juraszek AL, McGowan FX, Cowan DB, Del Nido P, Triedman JK, Berul CI, Walsh EP. Atrial remodeling after the Fontan operation. Am J Cardiol. 2009 Dec 15; 104(12):1737-42.
    View in: PubMed
  10. Kitahori K, He H, Kawata M, Cowan DB, Friehs I, Del Nido PJ, McGowan FX. Development of left ventricular diastolic dysfunction with preservation of ejection fraction during progression of infant right ventricular hypertrophy. Circ Heart Fail. 2009 Nov; 2(6):599-607.
    View in: PubMed
  11. Sill B, Hammer PE, Cowan DB. Optical mapping of Langendorff-perfused rat hearts. J Vis Exp. 2009; (30).
    View in: PubMed
  12. Toumpoulis IK, Oxford JT, Cowan DB, Anagnostopoulos CE, Rokkas CK, Chamogeorgakis TP, Angouras DC, Shemin RJ, Navab M, Ericsson M, Federman M, Levitsky S, McCully JD. Differential expression of collagen type V and XI alpha-1 in human ascending thoracic aortic aneurysms. Ann Thorac Surg. 2009 Aug; 88(2):506-13.
    View in: PubMed
  13. Choi YH, Cowan DB, Wahlers TC, Hetzer R, Del Nido PJ, Stamm C. Calcium sensitisation impairs diastolic relaxation in post-ischaemic myocardium: implications for the use of Ca(2+) sensitising inotropes after cardiac surgery. Eur J Cardiothorac Surg. 2010 Feb; 37(2):376-83.
    View in: PubMed
  14. Sill B, Alpatov IV, Pacak CA, Cowan DB. Implantation of engineered tissue in the rat heart. J Vis Exp. 2009; (28).
    View in: PubMed
  15. McCully JD, Bhasin MK, Daly C, Guerrero MC, Dillon S, Liberman TA, Cowan DB, Mably JD, McGowan FX, Levitsky S. Transcriptomic and proteomic analysis of global ischemia and cardioprotection in the rabbit heart. Physiol Genomics. 2009 Jul 9; 38(2):125-37.
    View in: PubMed
  16. Pacak CA, Cowan DB. Fabrication of myogenic engineered tissue constructs. J Vis Exp. 2009; (27).
    View in: PubMed
  17. Choi YH, Cowan DB, Moran AM, Colan SD, Stamm C, Takeuchi K, Friehs I, del Nido PJ, McGowan FX. Myocyte apoptosis occurs early during the development of pressure-overload hypertrophy in infant myocardium. J Thorac Cardiovasc Surg. 2009 Jun; 137(6):1356-62, 1362.e1-3.
    View in: PubMed
  18. Choi YH, Cowan DB, Nathan M, Poutias D, Stamm C, del Nido PJ, McGowan FX. Myocardial hypertrophy overrides the angiogenic response to hypoxia. PLoS One. 2008; 3(12):e4042.
    View in: PubMed
  19. McCully JD, Cowan DB, Pacak CA, Toumpoulis IK, Dayalan H, Levitsky S. Injection of isolated mitochondria during early reperfusion for cardioprotection. Am J Physiol Heart Circ Physiol. 2009 Jan; 296(1):H94-H105.
    View in: PubMed
  20. Roy N, Friehs I, Cowan DB, Zurakowski D, McGowan FX, del Nido PJ. Dopamine induces postischemic cardiomyocyte apoptosis in vivo: an effect ameliorated by propofol. Ann Thorac Surg. 2006 Dec; 82(6):2192-9.
    View in: PubMed
  21. Yoon S, Molloy MJ, Wu MP, Cowan DB, Gussoni E. C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia. Dev Biol. 2007 Jan 1; 301(1):70-81.
    View in: PubMed
  22. Tansey EE, Kwaku KF, Hammer PE, Cowan DB, Federman M, Levitsky S, McCully JD. Reduction and redistribution of gap and adherens junction proteins after ischemia and reperfusion. Ann Thorac Surg. 2006 Oct; 82(4):1472-9.
    View in: PubMed
  23. Cowan DB, McGowan FX. A paradigm shift in cardiac pacing therapy? Circulation. 2006 Sep 5; 114(10):986-8.
    View in: PubMed
  24. Choi YH, Stamm C, Hammer PE, Kwaku KF, Marler JJ, Friehs I, Jones M, Rader CM, Roy N, Eddy MT, Triedman JK, Walsh EP, McGowan FX, del Nido PJ, Cowan DB. Cardiac conduction through engineered tissue. Am J Pathol. 2006 Jul; 169(1):72-85.
    View in: PubMed
  25. Luxameechanporn T, Hadlock T, Shyu J, Cowan D, Faquin W, Varvares M. Successful myoblast transplantation in rat tongue reconstruction. Head Neck. 2006 Jun; 28(6):517-24.
    View in: PubMed
  26. Bayliss PE, Bellavance KL, Whitehead GG, Abrams JM, Aegerter S, Robbins HS, Cowan DB, Keating MT, O'Reilly T, Wood JM, Roberts TM, Chan J. Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish. Nat Chem Biol. 2006 May; 2(5):265-73.
    View in: PubMed
  27. Chen JN, Cowan DB, Mably JD. Cardiogenesis and the regulation of cardiac-specific gene expression. Heart Fail Clin. 2005 Jul; 1(2):157-70.
    View in: PubMed
  28. Nathan M, Friehs I, Choi YH, Cowan DB, Cao-Danh H, McGowan FX, del Nido PJ. Cyclosporin A but not FK-506 protects against dopamine-induced apoptosis in the stunned heart. Ann Thorac Surg. 2005 May; 79(5):1620-6.
    View in: PubMed
  29. Friehs I, Moran AM, Stamm C, Choi YH, Cowan DB, McGowan FX, del Nido PJ. Promoting angiogenesis protects severely hypertrophied hearts from ischemic injury. Ann Thorac Surg. 2004 Jun; 77(6):2004-10; discussion 2011.
    View in: PubMed
  30. Stamm C, Cowan DB. Endotoxemia and cardiac function. J Thorac Cardiovasc Surg. 2004 Jan; 127(1):305.
    View in: PubMed
  31. Bunaprasert T, Hadlock T, Marler J, Kobler J, Cowan D, Faquin W, Varvares M. Tissue engineered muscle implantation for tongue reconstruction: a preliminary report. Laryngoscope. 2003 Oct; 113(10):1792-7.
    View in: PubMed
  32. Cowan DB, Jones M, Garcia LM, Noria S, del Nido PJ, McGowan FX. Hypoxia and stretch regulate intercellular communication in vascular smooth muscle cells through reactive oxygen species formation. Arterioscler Thromb Vasc Biol. 2003 Oct 1; 23(10):1754-60.
    View in: PubMed
  33. Friehs I, Cao-Danh H, Stamm C, Cowan DB, McGowan FX, del Nido PJ. Postnatal increase in insulin-sensitive glucose transporter expression is associated with improved recovery of postischemic myocardial function. J Thorac Cardiovasc Surg. 2003 Jul; 126(1):263-71.
    View in: PubMed
  34. McCully JD, Wakiyama H, Cowan DB, Federman M, Parker RA, Levitsky S. Diazoxide amelioration of myocardial injury and mitochondrial damage during cardiac surgery. Ann Thorac Surg. 2002 Dec; 74(6):2138-45; discussion 2146.
    View in: PubMed
  35. Stamm C, Friehs I, Cowan DB, Cao-Danh H, Choi YH, Duebener LF, McGowan FX, del Nido PJ. Dopamine treatment of postischemic contractile dysfunction rapidly induces calcium-dependent pro-apoptotic signaling. Circulation. 2002 Sep 24; 106(12 Suppl 1):I290-8.
    View in: PubMed
  36. Chanani NK, Cowan DB, Takeuchi K, Poutias DN, Garcia LM, del Nido PJ, McGowan FX. Differential effects of amrinone and milrinone upon myocardial inflammatory signaling. Circulation. 2002 Sep 24; 106(12 Suppl 1):I284-9.
    View in: PubMed
  37. Munakata M, Stamm C, Friehs I, Zurakowski D, Cowan DB, Cao-Danh H, McGowan FX, del Nido PJ. Protective effects of protein kinase C during myocardial ischemia require activation of phosphatidyl-inositol specific phospholipase C. Ann Thorac Surg. 2002 Apr; 73(4):1236-45.
    View in: PubMed
  38. Wakiyama H, Cowan DB, Toyoda Y, Federman M, Levitsky S, McCully JD. Selective opening of mitochondrial ATP-sensitive potassium channels during surgically induced myocardial ischemia decreases necrosis and apoptosis. Eur J Cardiothorac Surg. 2002 Mar; 21(3):424-33.
    View in: PubMed
  39. Soriano SG, Cowan DB, Proctor MR, Scott RM. Levels of soluble adhesion molecules are elevated in the cerebrospinal fluid of children with moyamoya syndrome. Neurosurgery. 2002 Mar; 50(3):544-9.
    View in: PubMed
  40. Merante F, Altamentova SM, Mickle DA, Weisel RD, Thatcher BJ, Martin BM, Marshall JG, Tumiati LC, Cowan DB, Li RK. The characterization and purification of a human transcription factor modulating the glutathione peroxidase gene in response to oxygen tension. Mol Cell Biochem. 2002 Jan; 229(1-2):73-83.
    View in: PubMed
  41. Stamm C, Cowan DB, Friehs I, Noria S, del Nido PJ, McGowan FX. Rapid endotoxin-induced alterations in myocardial calcium handling: obligatory role of cardiac TNF-alpha. Anesthesiology. 2001 Dec; 95(6):1396-405.
    View in: PubMed
  42. Stamm C, Friehs I, Cowan DB, Moran AM, Cao-Danh H, Duebener LF, del Nido PJ, McGowan FX. Inhibition of tumor necrosis factor-alpha improves postischemic recovery of hypertrophied hearts. Circulation. 2001 Sep 18; 104(12 Suppl 1):I350-5.
    View in: PubMed
  43. Stamm C, Friehs I, Cowan DB, Cao-Danh H, Noria S, Munakata M, McGowan FX, del Nido PJ. Post-ischemic PKC inhibition impairs myocardial calcium handling and increases contractile protein calcium sensitivity. Cardiovasc Res. 2001 Jul; 51(1):108-21.
    View in: PubMed
  44. Cowan DB, Noria S, Stamm C, Garcia LM, Poutias DN, del Nido PJ, McGowan FX. Lipopolysaccharide internalization activates endotoxin-dependent signal transduction in cardiomyocytes. Circ Res. 2001 Mar 16; 88(5):491-8.
    View in: PubMed
  45. MacGowan GA, Du C, Cowan DB, Stamm C, McGowan FX, Solaro RJ, Koretsky AP, Del Nido PJ. Ischemic dysfunction in transgenic mice expressing troponin I lacking protein kinase C phosphorylation sites. Am J Physiol Heart Circ Physiol. 2001 Feb; 280(2):H835-43.
    View in: PubMed
  46. Takeuchi K, del Nido PJ, Poutias DN, Cowan DB, Munakata M, McGowan FX. Vesnarinone restores contractility and calcium handling in early endotoxemia. Circulation. 2000 Nov 7; 102(19 Suppl 3):III365-9.
    View in: PubMed
  47. Cowan DB, Poutias DN, Del Nido PJ, McGowan FX. CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin. Am J Physiol Heart Circ Physiol. 2000 Aug; 279(2):H619-29.
    View in: PubMed
  48. Noria S, Cowan DB, Gotlieb AI, Langille BL. Transient and steady-state effects of shear stress on endothelial cell adherens junctions. Circ Res. 1999 Sep 17; 85(6):504-14.
    View in: PubMed
  49. Takeuchi K, del Nido PJ, Ibrahim AE, Poutias DN, Glynn P, Cao-Danh H, Cowan DB, McGowan FX. Increased myocardial calcium cycling and reduced myofilament calcium sensitivity in early endotoxemia. Surgery. 1999 Aug; 126(2):231-8.
    View in: PubMed
  50. Takeuchi K, del Nido PJ, Ibrahim AE, Cao-Danh H, Friehs I, Glynn P, Poutias D, Cowan DB, McGowan FX. Vesnarinone and amrinone reduce the systemic inflammatory response syndrome. J Thorac Cardiovasc Surg. 1999 Feb; 117(2):375-82.
    View in: PubMed
  51. Cowan DB, Lye SJ, Langille BL. Regulation of vascular connexin43 gene expression by mechanical loads. Circ Res. 1998 Apr 20; 82(7):786-93.
    View in: PubMed
  52. Cowan DB, Langille BL. Cellular and molecular biology of vascular remodeling. Curr Opin Lipidol. 1996 Apr; 7(2):94-100.
    View in: PubMed
  53. Li RK, Cowan DB, Mickle DA, Weisel RD, Burton GW. Effect of vitamin E on human glutathione peroxidase (GSH-PX1) expression in cardiomyocytes. Free Radic Biol Med. 1996; 21(4):419-26.
    View in: PubMed
  54. Cowan DB, Weisel RD, Williams WG, Mickle DA. Identification of oxygen responsive elements in the 5'-flanking region of the human glutathione peroxidase gene. J Biol Chem. 1993 Dec 25; 268(36):26904-10.
    View in: PubMed
  55. Cowan DB, Weisel RD, Williams WG, Mickle DA. The regulation of glutathione peroxidase gene expression by oxygen tension in cultured human cardiomyocytes. J Mol Cell Cardiol. 1992 Apr; 24(4):423-33.
    View in: PubMed
  56. Cowan DB, Chaban G, Liew CC. The DNA-binding properties of a rat nuclear phosphoprotein. Biochem J. 1991 Mar 15; 274 ( Pt 3):687-91.
    View in: PubMed
Local representatives can answer questions about the Profiles website or help with editing a profile or issues with profile data. For assistance with this profile: HMS/HSDM faculty should contact Human Resources at faculty_serviceshms.harvard.edu.
Cowan's Networks
Click the "See All" links for more information and interactive visualizations!
Concepts
_
Co-Authors
_
Similar People
_
Same Department
Physical Neighbors
_