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Wayne G Shreffler, M.D., Ph.D.

TitleAssociate Professor of Pediatrics
InstitutionMassachusetts General Hospital
DepartmentPediatrics
AddressMassachusetts General Hospital
Pediatric-Allergy Section
55 Fruit St
Boston MA 02114
Phone617/726-6147
Fax6177265651

 Overview 
 overview
Our research program involves a combination of approaches, primarily with human samples and often in conjunction with clinical interventional or observational studies, to interrogate both the innate and adaptive immune responses to major dietary and aeroallergens.

Working together with leading clinical investigators around the world as part of the Consortium for Food Allergy Research and the Inner City Asthma Consortium and the newly established Food Allergy Research Center at MGH, we are adapting the use of polychromatic flow cytometry and peptide microarray-based humoral immune profiling and systems biology approaches to uniquely characterize the phenotype and function of allergen-specific T and B cell responses and the regulations of effector cells in pediatric food allergy and asthma.

Equally important is the effort to understand the mechanisms that influence early immune fate decisions to allergens and the intrinsic properties of those allergens that facilitate an allergic response and we are studying this in human and murine model systems.


 Mentoring 
 current student opportunities
Available: 08/01/13, Expires: 07/31/14

Approximately 0.8% of the U.S. population is allergic to peanuts and the are at risk for severe reactions. Over 50% of these individuals will have an allergic reaction to peanuts over a 2-year period. Strict avoidance of food allergens and ready access to self-injectable epinephrine are the only accepted management options. Peanut oral immunotherapy (PNOIT) significantly increases the dose of peanut that can be ingested without symptoms for most patients while the therapy is being actively administered, but long-lasting tolerance is not well defined. We are currently conducting an NIH-funded study of PNOIT to address mechanisms of tolerance. This project addresses how PNOIT affects the B cell repertoire in those patients and how those changes correlate to the heterogeneous clinical outcomes that are expected. The student’s role would be to work closely with a post-doctoral fellow to apply advanced BCR sequencing and cloning methods to the analysis of the B cell response to peanut antigens during PNOIT and relating those outcomes to the clinical response.


 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Leung J, Hundal NV, Katz AJ, Shreffler WG, Yuan Q, Butterworth CA, Hesterberg PE. Tolerance of baked milk in patients with cow's milk-mediated eosinophilic esophagitis. J Allergy Clin Immunol. 2013 Nov; 132(5):1215-1216.e1.
    View in: PubMed
  2. Zurzolo GA, Allen KJ, Taylor SL, Shreffler WG, Baumert JL, Tang ML, Gurrin LC, Mathai ML, Nordlee JA, Dunngalvin A, Hourihane JO. Peanut Allergen Threshold Study (PATS): validation of eliciting doses using a novel single-dose challenge protocol. Allergy Asthma Clin Immunol. 2013; 9(1):35.
    View in: PubMed
  3. Islam SA, Ling MF, Leung J, Shreffler WG, Luster AD. Identification of human CCR8 as a CCL18 receptor. J Exp Med. 2013 Sep 23; 210(10):1889-98.
    View in: PubMed
  4. Katz A, Virk Hundal N, Yuan Q, Shreffler W. Cow's Milk Allergy: A New Approach Needed? J Pediatr. 2013 Sep; 163(3):620-2.
    View in: PubMed
  5. Ruiter B, Shreffler WG. Innate immunostimulatory properties of allergens and their relevance to food allergy. Semin Immunopathol. 2012 Sep; 34(5):617-32.
    View in: PubMed
  6. Thyagarajan A, Jones SM, Calatroni A, Pons L, Kulis M, Woo CS, Kamalakannan M, Vickery BP, Scurlock AM, Wesley Burks A, Shreffler WG. Evidence of pathway-specific basophil anergy induced by peanut oral immunotherapy in peanut-allergic children. Clin Exp Allergy. 2012 Aug; 42(8):1197-205.
    View in: PubMed
  7. Ford LS, Bloom KA, Nowak-Wegrzyn AH, Shreffler WG, Masilamani M, Sampson HA. Basophil reactivity, wheal size, and immunoglobulin levels distinguish degrees of cow's milk tolerance. J Allergy Clin Immunol. 2013 Jan; 131(1):180-186.e3.
    View in: PubMed
  8. Burks AW, Jones SM, Wood RA, Fleischer DM, Sicherer SH, Lindblad RW, Stablein D, Henning AK, Vickery BP, Liu AH, Scurlock AM, Shreffler WG, Plaut M, Sampson HA. Oral immunotherapy for treatment of egg allergy in children. N Engl J Med. 2012 Jul 19; 367(3):233-43.
    View in: PubMed
  9. Ruiter B, Shreffler WG. The role of dendritic cells in food allergy. J Allergy Clin Immunol. 2012 Apr; 129(4):921-8.
    View in: PubMed
  10. Masilamani M, Commins S, Shreffler W. Determinants of food allergy. Immunol Allergy Clin North Am. 2012 Feb; 32(1):11-33.
    View in: PubMed
  11. Patil SU, Shreffler WG. Immunology in the Clinic Review Series; focus on allergies: basophils as biomarkers for assessing immune modulation. Clin Exp Immunol. 2012 Jan; 167(1):59-66.
    View in: PubMed
  12. Rosenfeld L, Shreffler W, Bardina L, Niggemann B, Wahn U, Sampson HA, Beyer K. Walnut allergy in peanut-allergic patients: significance of sequential epitopes of walnut homologous to linear epitopes of Ara h 1, 2 and 3 in relation to clinical reactivity. Int Arch Allergy Immunol. 2012; 157(3):238-45.
    View in: PubMed
  13. Shreffler WG, Radano M. Food allergy and complementary feeding. Nestle Nutr Workshop Ser Pediatr Program. 2011; 68:141-9; discussion 150-2.
    View in: PubMed
  14. Peng RD, Paigen B, Eggleston PA, Hagberg KA, Krevans M, Curtin-Brosnan J, Benson C, Shreffler WG, Matsui EC. Both the variability and level of mouse allergen exposure influence the phenotype of the immune response in workers at a mouse facility. J Allergy Clin Immunol. 2011 Aug; 128(2):390-396.e7.
    View in: PubMed
  15. Shreffler WG. Microarrayed recombinant allergens for diagnostic testing. J Allergy Clin Immunol. 2011 Apr; 127(4):843-9; quiz 850-1.
    View in: PubMed
  16. Kim EH, Bird JA, Kulis M, Laubach S, Pons L, Shreffler W, Steele P, Kamilaris J, Vickery B, Burks AW. Sublingual immunotherapy for peanut allergy: clinical and immunologic evidence of desensitization. J Allergy Clin Immunol. 2011 Mar; 127(3):640-6.e1.
    View in: PubMed
  17. Ditto AM, Neilsen CV, Neerukonda S, Shreffler WG, Bryce PJ. Clinical reactivity to raw peanut correlates with IgE binding to conformational epitopes of Ara h 1: a case report. Allergy. 2010 Nov; 65(11):1485-6.
    View in: PubMed
  18. Vereda A, Andreae DA, Lin J, Shreffler WG, Ibañez MD, Cuesta-Herranz J, Bardina L, Sampson HA. Identification of IgE sequential epitopes of lentil (Len c 1) by means of peptide microarray immunoassay. J Allergy Clin Immunol. 2010 Sep; 126(3):596-601.e1.
    View in: PubMed
  19. Blumchen K, Ulbricht H, Staden U, Dobberstein K, Beschorner J, de Oliveira LC, Shreffler WG, Sampson HA, Niggemann B, Wahn U, Beyer K. Oral peanut immunotherapy in children with peanut anaphylaxis. J Allergy Clin Immunol. 2010 Jul; 126(1):83-91.e1.
    View in: PubMed
  20. Sicherer SH, Wood RA, Stablein D, Burks AW, Liu AH, Jones SM, Fleischer DM, Leung DY, Grishin A, Mayer L, Shreffler W, Lindblad R, Sampson HA. Immunologic features of infants with milk or egg allergy enrolled in an observational study (Consortium of Food Allergy Research) of food allergy. J Allergy Clin Immunol. 2010 May; 125(5):1077-1083.e8.
    View in: PubMed
  21. Wang J, Lin J, Bardina L, Goldis M, Nowak-Wegrzyn A, Shreffler WG, Sampson HA. Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy. J Allergy Clin Immunol. 2010 Mar; 125(3):695-702, 702.e1-702.e6.
    View in: PubMed
  22. Järvinen KM, Amalanayagam S, Shreffler WG, Noone S, Sicherer SH, Sampson HA, Nowak-Wegrzyn A. Epinephrine treatment is infrequent and biphasic reactions are rare in food-induced reactions during oral food challenges in children. J Allergy Clin Immunol. 2009 Dec; 124(6):1267-72.
    View in: PubMed
  23. Lin J, Bardina L, Shreffler WG, Andreae DA, Ge Y, Wang J, Bruni FM, Fu Z, Han Y, Sampson HA. Development of a novel peptide microarray for large-scale epitope mapping of food allergens. J Allergy Clin Immunol. 2009 Aug; 124(2):315-22, 322.e1-3.
    View in: PubMed
  24. Jones SM, Pons L, Roberts JL, Scurlock AM, Perry TT, Kulis M, Shreffler WG, Steele P, Henry KA, Adair M, Francis JM, Durham S, Vickery BP, Zhong X, Burks AW. Clinical efficacy and immune regulation with peanut oral immunotherapy. J Allergy Clin Immunol. 2009 Aug; 124(2):292-300, 300.e1-97.
    View in: PubMed
  25. Gern JE, Visness CM, Gergen PJ, Wood RA, Bloomberg GR, O'Connor GT, Kattan M, Sampson HA, Witter FR, Sandel MT, Shreffler WG, Wright RJ, Arbes SJ, Busse WW. The Urban Environment and Childhood Asthma (URECA) birth cohort study: design, methods, and study population. BMC Pulm Med. 2009; 9:17.
    View in: PubMed
  26. Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG. Allergen-specific basophil suppression associated with clinical tolerance in patients with milk allergy. J Allergy Clin Immunol. 2009 Apr; 123(4):789-94.e20.
    View in: PubMed
  27. Shreffler WG. The perfectly potent peanut. J Allergy Clin Immunol. 2009 Feb; 123(2):352-3.
    View in: PubMed
  28. Lin J, Bardina L, Shreffler WG. Microarrayed allergen molecules for diagnostics of allergy. Methods Mol Biol. 2009; 524:259-72.
    View in: PubMed
  29. Shreffler WG, Wanich N, Moloney M, Nowak-Wegrzyn A, Sampson HA. Association of allergen-specific regulatory T cells with the onset of clinical tolerance to milk protein. J Allergy Clin Immunol. 2009 Jan; 123(1):43-52.e7.
    View in: PubMed
  30. Moloney M, Shreffler WG. Basic science for the practicing physician: flow cytometry and cell sorting. Ann Allergy Asthma Immunol. 2008 Nov; 101(5):544-9.
    View in: PubMed
  31. Lemon-Mulé H, Sampson HA, Sicherer SH, Shreffler WG, Noone S, Nowak-Wegrzyn A. Immunologic changes in children with egg allergy ingesting extensively heated egg. J Allergy Clin Immunol. 2008 Nov; 122(5):977-983.e1.
    View in: PubMed
  32. Cerecedo I, Zamora J, Shreffler WG, Lin J, Bardina L, Dieguez MC, Wang J, Muriel A, de la Hoz B, Sampson HA. Mapping of the IgE and IgG4 sequential epitopes of milk allergens with a peptide microarray-based immunoassay. J Allergy Clin Immunol. 2008 Sep; 122(3):589-94.
    View in: PubMed
  33. Nowak-Wegrzyn A, Bloom KA, Sicherer SH, Shreffler WG, Noone S, Wanich N, Sampson HA. Tolerance to extensively heated milk in children with cow's milk allergy. J Allergy Clin Immunol. 2008 Aug; 122(2):342-7, 347.e1-2.
    View in: PubMed
  34. Berin MC, Shreffler WG. T(H)2 adjuvants: implications for food allergy. J Allergy Clin Immunol. 2008 Jun; 121(6):1311-20; quiz 1321-2.
    View in: PubMed
  35. Hyman SJ, Shreffler WG, Rapaport R. Type 1 diabetes, autoimmune thyroid disease, and chronic urticaria. Pediatr Diabetes. 2008 Oct; 9(5):508-11.
    View in: PubMed
  36. Flinterman AE, Knol EF, Lencer DA, Bardina L, den Hartog Jager CF, Lin J, Pasmans SG, Bruijnzeel-Koomen CA, Sampson HA, van Hoffen E, Shreffler WG. Peanut epitopes for IgE and IgG4 in peanut-sensitized children in relation to severity of peanut allergy. J Allergy Clin Immunol. 2008 Mar; 121(3):737-743.e10.
    View in: PubMed
  37. Shreffler WG, Visness CM, Burger M, Cruikshank WW, Lederman HM, de la Morena M, Grindle K, Calatroni A, Sampson HA, Gern JE. Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study. BMC Immunol. 2006; 7:29.
    View in: PubMed
  38. Shreffler WG, Castro RR, Kucuk ZY, Charlop-Powers Z, Grishina G, Yoo S, Burks AW, Sampson HA. The major glycoprotein allergen from Arachis hypogaea, Ara h 1, is a ligand of dendritic cell-specific ICAM-grabbing nonintegrin and acts as a Th2 adjuvant in vitro. J Immunol. 2006 Sep 15; 177(6):3677-85.
    View in: PubMed
  39. Li H, Nowak-Wegrzyn A, Charlop-Powers Z, Shreffler W, Chehade M, Thomas S, Roda G, Dahan S, Sperber K, Berin MC. Transcytosis of IgE-antigen complexes by CD23a in human intestinal epithelial cells and its role in food allergy. Gastroenterology. 2006 Jul; 131(1):47-58.
    View in: PubMed
  40. Shreffler WG, Charlop-Powers Z, Sicherer SH. Lack of association of HLA class II alleles with peanut allergy. Ann Allergy Asthma Immunol. 2006 Jun; 96(6):865-9.
    View in: PubMed
  41. Shreffler WG. Evaluation of basophil activation in food allergy: present and future applications. Curr Opin Allergy Clin Immunol. 2006 Jun; 6(3):226-33.
    View in: PubMed
  42. Knight AK, Shreffler WG, Sampson HA, Sicherer SH, Noone S, Mofidi S, Nowak-Wegrzyn A. Skin prick test to egg white provides additional diagnostic utility to serum egg white-specific IgE antibody concentration in children. J Allergy Clin Immunol. 2006 Apr; 117(4):842-7.
    View in: PubMed
  43. Shreffler WG, Lencer DA, Bardina L, Sampson HA. IgE and IgG4 epitope mapping by microarray immunoassay reveals the diversity of immune response to the peanut allergen, Ara h 2. J Allergy Clin Immunol. 2005 Oct; 116(4):893-9.
    View in: PubMed
  44. Shreffler WG, Beyer K, Chu TH, Burks AW, Sampson HA. Microarray immunoassay: association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes. J Allergy Clin Immunol. 2004 Apr; 113(4):776-82.
    View in: PubMed
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