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Robert Neil Husson, M.D.

TitleProfessor of Pediatrics
InstitutionChildren's Hospital Boston
DepartmentPediatrics
AddressChildrens Hospital
Infectious Diseases, Enders 761
300 Longwood Ave
Boston MA 02115
Phone617/919-2900
Fax617/730-0254

 Overview 
 overview
My laboratory is interested in the pathogenesis of Mycobacterium tuberculosis infection. Our research has focused on the mechanisms by which the bacterium adapts to its environment. Our investigations have centered on analysis of transcription regulation by alternative sigma factors of M. tuberculosis, and characterization of signal transduction in this organism mediated by serine/threonine protein kinases.


 Mentoring 
 current student opportunities
Available: 06/01/12, Expires: 09/30/14

Laboratory research investigating signaling pathways and transcription regulation in Mycobacterium tuberculosis. Projects involve basic molecular mechanisms of these processes and investigation of their relevance to disease pathogenesis. Additional research in the laboratory related to screening for new TB drugs.


 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Schifano JM, Edifor R, Sharp JD, Ouyang M, Konkimalla A, Husson RN, Woychik NA. Mycobacterial toxin MazF-mt6 inhibits translation through cleavage of 23S rRNA at the ribosomal A site. Proc Natl Acad Sci U S A. 2013 May 21; 110(21):8501-6.
    View in: PubMed
  2. Chou MF, Prisic S, Lubner JM, Church GM, Husson RN, Schwartz D. Using bacteria to determine protein kinase specificity and predict target substrates. PLoS One. 2012; 7(12):e52747.
    View in: PubMed
  3. Sharp JD, Cruz JW, Raman S, Inouye M, Husson RN, Woychik NA. Growth and translation inhibition through sequence-specific RNA binding by Mycobacterium tuberculosis VapC toxin. J Biol Chem. 2012 Apr 13; 287(16):12835-47.
    View in: PubMed
  4. Ymele-Leki P, Cao S, Sharp J, Lambert KG, McAdam AJ, Husson RN, Tamayo G, Clardy J, Watnick PI. A high-throughput screen identifies a new natural product with broad-spectrum antibacterial activity. PLoS One. 2012; 7(2):e31307.
    View in: PubMed
  5. Peters JM, Madhavan VL, Kazacos KR, Husson RN, Dangoudoubiyam S, Soul JS. Good outcome with early empiric treatment of neural larva migrans due to Baylisascaris procyonis. Pediatrics. 2012 Mar; 129(3):e806-11.
    View in: PubMed
  6. Mir M, Asong J, Li X, Cardot J, Boons GJ, Husson RN. The extracytoplasmic domain of the Mycobacterium tuberculosis Ser/Thr kinase PknB binds specific muropeptides and is required for PknB localization. PLoS Pathog. 2011 Jul; 7(7):e1002182.
    View in: PubMed
  7. Kang CM, Jahng WJ, Husson RN, Lee SH. A modified immunoblot method to identify substrates of protein kinases. J Microbiol. 2011 Jun; 49(3):499-501.
    View in: PubMed
  8. Prisic S, Dankwa S, Schwartz D, Chou MF, Locasale JW, Kang CM, Bemis G, Church GM, Steen H, Husson RN. Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Proc Natl Acad Sci U S A. 2010 Apr 20; 107(16):7521-6.
    View in: PubMed
  9. Pandey AK, Raman S, Proff R, Joshi S, Kang CM, Rubin EJ, Husson RN, Sassetti CM. Nitrile-inducible gene expression in mycobacteria. Tuberculosis (Edinb). 2009 Jan; 89(1):12-6.
    View in: PubMed
  10. Park ST, Kang CM, Husson RN. Regulation of the SigH stress response regulon by an essential protein kinase in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2008 Sep 2; 105(35):13105-10.
    View in: PubMed
  11. Zhu L, Phadtare S, Nariya H, Ouyang M, Husson RN, Inouye M. The mRNA interferases, MazF-mt3 and MazF-mt7 from Mycobacterium tuberculosis target unique pentad sequences in single-stranded RNA. Mol Microbiol. 2008 Aug; 69(3):559-69.
    View in: PubMed
  12. Kang CM, Nyayapathy S, Lee JY, Suh JW, Husson RN. Wag31, a homologue of the cell division protein DivIVA, regulates growth, morphology and polar cell wall synthesis in mycobacteria. Microbiology. 2008 Mar; 154(Pt 3):725-35.
    View in: PubMed
  13. Song T, Song SE, Raman S, Anaya M, Husson RN. Critical role of a single position in the -35 element for promoter recognition by Mycobacterium tuberculosis SigE and SigH. J Bacteriol. 2008 Mar; 190(6):2227-30.
    View in: PubMed
  14. Williams DL, Pittman TL, Deshotel M, Oby-Robinson S, Smith I, Husson R. Molecular basis of the defective heat stress response in Mycobacterium leprae. J Bacteriol. 2007 Dec; 189(24):8818-27.
    View in: PubMed
  15. Husson RN. Leaving on the lights: host-specific derepression of Mycobacterium tuberculosis gene expression by anti-sigma factor gene mutations. Mol Microbiol. 2006 Dec; 62(5):1217-9.
    View in: PubMed
  16. Raman S, Puyang X, Cheng TY, Young DC, Moody DB, Husson RN. Mycobacterium tuberculosis SigM positively regulates Esx secreted protein and nonribosomal peptide synthetase genes and down regulates virulence-associated surface lipid synthesis. J Bacteriol. 2006 Dec; 188(24):8460-8.
    View in: PubMed
  17. Hahn MY, Raman S, Anaya M, Husson RN. The Mycobacterium tuberculosis extracytoplasmic-function sigma factor SigL regulates polyketide synthases and secreted or membrane proteins and is required for virulence. J Bacteriol. 2005 Oct; 187(20):7062-71.
    View in: PubMed
  18. Kang CM, Abbott DW, Park ST, Dascher CC, Cantley LC, Husson RN. The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes Dev. 2005 Jul 15; 19(14):1692-704.
    View in: PubMed
  19. Kovacs A, Cowles MK, Britto P, Capparelli E, Fowler MG, Moye J, McIntosh K, Rathore MH, Pitt J, Husson RN. Pharmacokinetics of didanosine and drug resistance mutations in infants exposed to zidovudine during gestation or postnatally and treated with didanosine or zidovudine in the first three months of life. Pediatr Infect Dis J. 2005 Jun; 24(6):503-9.
    View in: PubMed
  20. Raman S, Hazra R, Dascher CC, Husson RN. Transcription regulation by the Mycobacterium tuberculosis alternative sigma factor SigD and its role in virulence. J Bacteriol. 2004 Oct; 186(19):6605-16.
    View in: PubMed
  21. Lerman SJ, Bernardo J, Daly JS, Husson R. Tuberculosis prevention in college students. J Am Coll Health. 2004 Sep-Oct; 53(2):53-8.
    View in: PubMed
  22. Malley R, Stack AM, Husson RN, Thompson CM, Fleisher GR, Saladino RA. Development of a model of focal pneumococcal pneumonia in young rats. J Immune Based Ther Vaccines. 2004 Jan 23; 2(1):2.
    View in: PubMed
  23. Song T, Dove SL, Lee KH, Husson RN. RshA, an anti-sigma factor that regulates the activity of the mycobacterial stress response sigma factor SigH. Mol Microbiol. 2003 Nov; 50(3):949-59.
    View in: PubMed
  24. Raman S, Song T, Puyang X, Bardarov S, Jacobs WR, Husson RN. The alternative sigma factor SigH regulates major components of oxidative and heat stress responses in Mycobacterium tuberculosis. J Bacteriol. 2001 Oct; 183(20):6119-25.
    View in: PubMed
  25. Hazra R, Floyd MM, Sloutsky A, Husson RN. Novel mycobacterium related to Mycobacterium triplex as a cause of cervical lymphadenitis. J Clin Microbiol. 2001 Apr; 39(4):1227-30.
    View in: PubMed
  26. Hazra R, Lee SH, Maslow JN, Husson RN. Related strains of Mycobacterium avium cause disease in children with AIDS and in children with lymphadenitis. J Infect Dis. 2000 Apr; 181(4):1298-303.
    View in: PubMed
  27. Robson CD, Hazra R, Barnes PD, Robertson RL, Jones D, Husson RN. Nontuberculous mycobacterial infection of the head and neck in immunocompetent children: CT and MR findings. AJNR Am J Neuroradiol. 1999 Nov-Dec; 20(10):1829-35.
    View in: PubMed
  28. DeVincenzo JP, Berning SE, Peloquin CA, Husson RN. Multidrug-resistant tuberculosis meningitis: clinical problems and concentrations of second-line antituberculous medications. Ann Pharmacother. 1999 Nov; 33(11):1184-8.
    View in: PubMed
  29. Fernandes ND, Wu QL, Kong D, Puyang X, Garg S, Husson RN. A mycobacterial extracytoplasmic sigma factor involved in survival following heat shock and oxidative stress. J Bacteriol. 1999 Jul; 181(14):4266-74.
    View in: PubMed
  30. Rubin EJ, Akerley BJ, Novik VN, Lampe DJ, Husson RN, Mekalanos JJ. In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria. Proc Natl Acad Sci U S A. 1999 Feb 16; 96(4):1645-50.
    View in: PubMed
  31. Hazra R, Robson CD, Perez-Atayde AR, Husson RN. Lymphadenitis due to nontuberculous mycobacteria in children: presentation and response to therapy. Clin Infect Dis. 1999 Jan; 28(1):123-9.
    View in: PubMed
  32. Chong CY, Husson RN. Lack of acceptance of guidelines for prevention of disseminated Mycobacterium avium complex infection in infants and children infected with human immunodeficiency virus. Pediatr Infect Dis J. 1998 Dec; 17(12):1131-5.
    View in: PubMed
  33. Husson RN. Homologous recombination in Mycobacterium smegmatis. Screening methods for detection of gene replacement. Methods Mol Biol. 1998; 101:199-206.
    View in: PubMed
  34. Wu QL, Kong D, Lam K, Husson RN. A mycobacterial extracytoplasmic function sigma factor involved in survival following stress. J Bacteriol. 1997 May; 179(9):2922-9.
    View in: PubMed
  35. Husson RN, Lan Y, Kojima E, Venzon D, Mitsuya H, McIntosh K. Vertical transmission of human immunodeficiency virus type 1: autologous neutralizing antibody, virus load, and virus phenotype. J Pediatr. 1995 Jun; 126(6):865-71.
    View in: PubMed
  36. Mueller BU, Butler KM, Stocker VL, Balis FM, Brouwers P, Jarosinski P, Husson RN, Lewis LL, Venzon D, Pizzo PA. Clinical and pharmacokinetic evaluation of long-term therapy with didanosine in children with HIV infection. Pediatrics. 1994 Nov; 94(5):724-31.
    View in: PubMed
  37. Mueller BU, Pizzo PA, Farley M, Husson RN, Goldsmith J, Kovacs A, Woods L, Ono J, Church JA, Brouwers P, et al. Pharmacokinetic evaluation of the combination of zidovudine and didanosine in children with human immunodeficiency virus infection. J Pediatr. 1994 Jul; 125(1):142-6.
    View in: PubMed
  38. Husson RN, Ross LA, Sandelli S, Inderlied CB, Venzon D, Lewis LL, Woods L, Conville PS, Witebsky FG, Pizzo PA. Orally administered clarithromycin for the treatment of systemic Mycobacterium avium complex infection in children with acquired immunodeficiency syndrome. J Pediatr. 1994 May; 124(5 Pt 1):807-14.
    View in: PubMed
  39. Husson RN, Mueller BU, Farley M, Woods L, Kovacs A, Goldsmith JC, Ono J, Lewis LL, Balis FM, Brouwers P, et al. Zidovudine and didanosine combination therapy in children with human immunodeficiency virus infection. Pediatrics. 1994 Feb; 93(2):316-22.
    View in: PubMed
  40. Aldovini A, Husson RN, Young RA. The uraA locus and homologous recombination in Mycobacterium bovis BCG. J Bacteriol. 1993 Nov; 175(22):7282-9.
    View in: PubMed
  41. Husson RN, Shirasaka T, Butler KM, Pizzo PA, Mitsuya H. High-level resistance to zidovudine but not to zalcitabine or didanosine in human immunodeficiency virus from children receiving antiretroviral therapy. J Pediatr. 1993 Jul; 123(1):9-16.
    View in: PubMed
  42. Butler KM, Venzon D, Henry N, Husson RN, Mueller BU, Balis FM, Jacobsen F, Lewis LL, Pizzo PA. Pancreatitis in human immunodeficiency virus-infected children receiving dideoxyinosine. Pediatrics. 1993 Apr; 91(4):747-51.
    View in: PubMed
  43. Robert-Guroff M, Roilides E, Muldoon R, Venzon D, Husson R, Marshall D, Gallo RC, Pizzo PA. Human immunodeficiency virus (HIV) type 1 strain MN neutralizing antibody in HIV-infected children: correlation with clinical status and prognostic value. J Infect Dis. 1993 Mar; 167(3):538-46.
    View in: PubMed
  44. Shirasaka T, Yarchoan R, O'Brien MC, Husson RN, Anderson BD, Kojima E, Shimada T, Broder S, Mitsuya H. Changes in drug sensitivity of human immunodeficiency virus type 1 during therapy with azidothymidine, dideoxycytidine, and dideoxyinosine: an in vitro comparative study. Proc Natl Acad Sci U S A. 1993 Jan 15; 90(2):562-6.
    View in: PubMed
  45. Husson RN, Saini R, Lewis LL, Butler KM, Patronas N, Pizzo PA. Cerebral artery aneurysms in children infected with human immunodeficiency virus. J Pediatr. 1992 Dec; 121(6):927-30.
    View in: PubMed
  46. Lewis LL, Butler KM, Husson RN, Mueller BU, Fowler CL, Steinberg SM, Pizzo PA. Defining the population of human immunodeficiency virus-infected children at risk for Mycobacterium avium-intracellulare infection. J Pediatr. 1992 Nov; 121(5 Pt 1):677-83.
    View in: PubMed
  47. Mueller BU, Jacobsen F, Butler KM, Husson RN, Lewis LL, Pizzo PA. Combination treatment with azidothymidine and granulocyte colony-stimulating factor in children with human immunodeficiency virus infection. J Pediatr. 1992 Nov; 121(5 Pt 1):797-802.
    View in: PubMed
  48. Husson RN, Chung Y, Mordenti J, Butler KM, Chen S, Duliege AM, Brouwers P, Jarosinski P, Mueller BU, Ammann A, et al. Phase I study of continuous-infusion soluble CD4 as a single agent and in combination with oral dideoxyinosine therapy in children with symptomatic human immunodeficiency virus infection. J Pediatr. 1992 Oct; 121(4):627-33.
    View in: PubMed
  49. Mueller BU, Butler KM, Higham MC, Husson RN, Montrella KA, Pizzo PA, Feuerstein IM, Manjunath K. Smooth muscle tumors in children with human immunodeficiency virus infection. Pediatrics. 1992 Sep; 90(3):460-3.
    View in: PubMed
  50. Butler KM, Husson RN, Lewis LL, Mueller BU, Venzon D, Pizzo PA. CD4 status and P24 antigenemia. Are they useful predictors of survival in HIV-infected children receiving antiretroviral therapy? Am J Dis Child. 1992 Aug; 146(8):932-6.
    View in: PubMed
  51. Roilides E, Butler KM, Husson RN, Mueller BU, Lewis LL, Pizzo PA. Pseudomonas infections in children with human immunodeficiency virus infection. Pediatr Infect Dis J. 1992 Jul; 11(7):547-53.
    View in: PubMed
  52. Husson RN, Pizzo PA. The use of nucleoside analogues in the treatment of HIV-infected children. AIDS Res Hum Retroviruses. 1992 Jun; 8(6):1059-64.
    View in: PubMed
  53. Butler KM, De Smet MD, Husson RN, Mueller B, Manjunath K, Montrella K, Lovato G, Jarosinski P, Nussenblatt RB, Pizzo PA. Treatment of aggressive cytomegalovirus retinitis with ganciclovir in combination with foscarnet in a child infected with human immunodeficiency virus. J Pediatr. 1992 Mar; 120(3):483-6.
    View in: PubMed
  54. Whitcup SM, Butler KM, Caruso R, de Smet MD, Rubin B, Husson RN, Lopez JS, Belfort R, Pizzo PA, Nussenblatt RB. Retinal toxicity in human immunodeficiency virus-infected children treated with 2',3'-dideoxyinosine. Am J Ophthalmol. 1992 Jan 15; 113(1):1-7.
    View in: PubMed
  55. Balis FM, Pizzo PA, Butler KM, Hawkins ME, Brouwers P, Husson RN, Jacobsen F, Blaney SM, Gress J, Jarosinski P, et al. Clinical pharmacology of 2',3'-dideoxyinosine in human immunodeficiency virus-infected children. J Infect Dis. 1992 Jan; 165(1):99-104.
    View in: PubMed
  56. Mueller BU, Butler KM, Husson RN, Pizzo PA. Pneumocystis carinii pneumonia despite prophylaxis in children with human immunodeficiency virus infection. J Pediatr. 1991 Dec; 119(6):992-4.
    View in: PubMed
  57. Roilides E, Marshall D, Venzon D, Butler K, Husson R, Pizzo PA. Bacterial infections in human immunodeficiency virus type 1-infected children: the impact of central venous catheters and antiretroviral agents. Pediatr Infect Dis J. 1991 Nov; 10(11):813-9.
    View in: PubMed
  58. Butler KM, Husson RN, Balis FM, Brouwers P, Eddy J, el-Amin D, Gress J, Hawkins M, Jarosinski P, Moss H, et al. Dideoxyinosine in children with symptomatic human immunodeficiency virus infection. N Engl J Med. 1991 Jan 17; 324(3):137-44.
    View in: PubMed
  59. Butler KM, Husson RN, Balis FM. Drug trial benefited HIV children. Nurs Times. 1991 Jan 30-Feb 5; 87(5):10.
    View in: PubMed
  60. Pizzo PA, Butler K, Balis F, Brouwers E, Hawkins M, Eddy J, Einloth M, Falloon J, Husson R, Jarosinski P, et al. Dideoxycytidine alone and in an alternating schedule with zidovudine in children with symptomatic human immunodeficiency virus infection. J Pediatr. 1990 Nov; 117(5):799-808.
    View in: PubMed
  61. Husson RN, Comeau AM, Hoff R. Diagnosis of human immunodeficiency virus infection in infants and children. Pediatrics. 1990 Jul; 86(1):1-10.
    View in: PubMed
  62. Husson RN, James BE, Young RA. Gene replacement and expression of foreign DNA in mycobacteria. J Bacteriol. 1990 Feb; 172(2):519-24.
    View in: PubMed
  63. Boom WH, Husson RN, Young RA, David JR, Piessens WF. In vivo and in vitro characterization of murine T-cell clones reactive to Mycobacterium tuberculosis. Infect Immun. 1987 Sep; 55(9):2223-9.
    View in: PubMed
  64. Husson RN, Young RA. Genes for the major protein antigens of Mycobacterium tuberculosis: the etiologic agents of tuberculosis and leprosy share an immunodominant antigen. Proc Natl Acad Sci U S A. 1987 Mar; 84(6):1679-83.
    View in: PubMed
  65. Oftung F, Mustafa AS, Husson R, Young RA, Godal T. Human T cell clones recognize two abundant Mycobacterium tuberculosis protein antigens expressed in Escherichia coli. J Immunol. 1987 Feb 1; 138(3):927-31.
    View in: PubMed
  66. Orkin SH, Kolodner R, Michelson A, Husson R. Cloning and direct examination of a structurally abnormal human beta 0-thalassemia globin gene. Proc Natl Acad Sci U S A. 1980 Jun; 77(6):3558-62.
    View in: PubMed
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