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Robin Reed, PH.D.

TitleProfessor of Cell Biology
InstitutionHarvard Medical School
DepartmentCell Biology
AddressDept of Cell Biology
Lhrrb 501
45 Shattuck St
Boston MA 02115
Phone617/432-2844
Fax617/432-3091

 Mentoring 
 completed student projects
Efficient Isolation of the Splicosomal Complex Using AdML pre-mRNA
Part Time/Continuation, 01/03/01 - 05/31/01
Construction of a Generic Intron Cassette for Achieving Maximal Levels of Splicing and mRNA Export
Summer/Continuation, 06/18/00 - 08/31/00
Construction of a Generic Intron Cassette for Achieving Maximal Levels of Splicing and mRNA Export
Part Time, 04/17/00 - 06/16/00
Efficient Isolation of the Spliceosomal Complex Using AdML pre-mrna and MS2 Coat/Maltose Binding Fusion Protein
Part Time/Continuation, 09/05/00 - 12/22/00

 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Lei H, Zhai B, Yin S, Gygi S, Reed R. Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export. Nucleic Acids Res. 2013 Feb 1; 41(4):2517-25.
    View in: PubMed
  2. Yamazaki T, Chen S, Yu Y, Yan B, Haertlein TC, Carrasco MA, Tapia JC, Zhai B, Das R, Lalancette-Hebert M, Sharma A, Chandran S, Sullivan G, Nishimura AL, Shaw CE, Gygi SP, Shneider NA, Maniatis T, Reed R. FUS-SMN Protein Interactions Link the Motor Neuron Diseases ALS and SMA. Cell Rep. 2012 Oct 25; 2(4):799-806.
    View in: PubMed
  3. Folco EG, Lee CS, Dufu K, Yamazaki T, Reed R. The proteins PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export. PLoS One. 2012; 7(8):e43804.
    View in: PubMed
  4. Folco EG, Lei H, Hsu JL, Reed R. Small-scale nuclear extracts for functional assays of gene-expression machineries. J Vis Exp. 2012; (64).
    View in: PubMed
  5. Wang L, Lawrence MS, Wan Y, Stojanov P, Sougnez C, Stevenson K, Werner L, Sivachenko A, DeLuca DS, Zhang L, Zhang W, Vartanov AR, Fernandes SM, Goldstein NR, Folco EG, Cibulskis K, Tesar B, Sievers QL, Shefler E, Gabriel S, Hacohen N, Reed R, Meyerson M, Golub TR, Lander ES, Neuberg D, Brown JR, Getz G, Wu CJ. SF3B1 and other novel cancer genes in chronic lymphocytic leukemia. N Engl J Med. 2011 Dec 29; 365(26):2497-506.
    View in: PubMed
  6. Lei H, Dias AP, Reed R. Export and stability of naturally intronless mRNAs require specific coding region sequences and the TREX mRNA export complex. Proc Natl Acad Sci U S A. 2011 Nov 1; 108(44):17985-90.
    View in: PubMed
  7. Folco EG, Coil KE, Reed R. The anti-tumor drug E7107 reveals an essential role for SF3b in remodeling U2 snRNP to expose the branch point-binding region. Genes Dev. 2011 Mar 1; 25(5):440-4.
    View in: PubMed
  8. Dias AP, Dufu K, Lei H, Reed R. A role for TREX components in the release of spliced mRNA from nuclear speckle domains. Nat Commun. 2010; 1:97.
    View in: PubMed
  9. Dufu K, Livingstone MJ, Seebacher J, Gygi SP, Wilson SA, Reed R. ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex. Genes Dev. 2010 Sep 15; 24(18):2043-53.
    View in: PubMed
  10. Yu Y, Das R, Folco EG, Reed R. A model in vitro system for co-transcriptional splicing. Nucleic Acids Res. 2010 Nov; 38(21):7570-8.
    View in: PubMed
  11. Lee CS, Dias AP, Jedrychowski M, Patel AH, Hsu JL, Reed R. Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Nucleic Acids Res. 2008 Aug; 36(14):4708-18.
    View in: PubMed
  12. Valencia P, Dias AP, Reed R. Splicing promotes rapid and efficient mRNA export in mammalian cells. Proc Natl Acad Sci U S A. 2008 Mar 4; 105(9):3386-91.
    View in: PubMed
  13. Das R, Yu J, Zhang Z, Gygi MP, Krainer AR, Gygi SP, Reed R. SR proteins function in coupling RNAP II transcription to pre-mRNA splicing. Mol Cell. 2007 Jun 22; 26(6):867-81.
    View in: PubMed
  14. Ganapathi KA, Austin KM, Lee CS, Dias A, Malsch MM, Reed R, Shimamura A. The human Shwachman-Diamond syndrome protein, SBDS, associates with ribosomal RNA. Blood. 2007 Sep 1; 110(5):1458-65.
    View in: PubMed
  15. Hims MM, Ibrahim EC, Leyne M, Mull J, Liu L, Lazaro C, Shetty RS, Gill S, Gusella JF, Reed R, Slaugenhaupt SA. Therapeutic potential and mechanism of kinetin as a treatment for the human splicing disease familial dysautonomia. J Mol Med (Berl). 2007 Feb; 85(2):149-61.
    View in: PubMed
  16. Ibrahim EC, Hims MM, Shomron N, Burge CB, Slaugenhaupt SA, Reed R. Weak definition of IKBKAP exon 20 leads to aberrant splicing in familial dysautonomia. Hum Mutat. 2007 Jan; 28(1):41-53.
    View in: PubMed
  17. Cheng H, Dufu K, Lee CS, Hsu JL, Dias A, Reed R. Human mRNA export machinery recruited to the 5' end of mRNA. Cell. 2006 Dec 29; 127(7):1389-400.
    View in: PubMed
  18. Das R, Dufu K, Romney B, Feldt M, Elenko M, Reed R. Functional coupling of RNAP II transcription to spliceosome assembly. Genes Dev. 2006 May 1; 20(9):1100-9.
    View in: PubMed
  19. Masuda S, Das R, Cheng H, Hurt E, Dorman N, Reed R. Recruitment of the human TREX complex to mRNA during splicing. Genes Dev. 2005 Jul 1; 19(13):1512-7.
    View in: PubMed
  20. Reed R, Cheng H. TREX, SR proteins and export of mRNA. Curr Opin Cell Biol. 2005 Jun; 17(3):269-73.
    View in: PubMed
  21. Ibrahim EC, Schaal TD, Hertel KJ, Reed R, Maniatis T. Serine/arginine-rich protein-dependent suppression of exon skipping by exonic splicing enhancers. Proc Natl Acad Sci U S A. 2005 Apr 5; 102(14):5002-7.
    View in: PubMed
  22. Ferraiuolo MA, Lee CS, Ler LW, Hsu JL, Costa-Mattioli M, Luo MJ, Reed R, Sonenberg N. A nuclear translation-like factor eIF4AIII is recruited to the mRNA during splicing and functions in nonsense-mediated decay. Proc Natl Acad Sci U S A. 2004 Mar 23; 101(12):4118-23.
    View in: PubMed
  23. Hurt E, Luo MJ, Röther S, Reed R, Strässer K. Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex. Proc Natl Acad Sci U S A. 2004 Feb 17; 101(7):1858-62.
    View in: PubMed
  24. Rodríguez-Navarro S, Fischer T, Luo MJ, Antúnez O, Brettschneider S, Lechner J, Pérez-Ortín JE, Reed R, Hurt E. Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. Cell. 2004 Jan 9; 116(1):75-86.
    View in: PubMed
  25. Zhou Z, Reed R. Purification of functional RNA-protein complexes using MS2-MBP. Curr Protoc Mol Biol. 2003 Aug; Chapter 27:Unit 27.3.
    View in: PubMed
  26. Lee CS, Das R, Reed R. Agarose gel separation/isolation of RNA-protein complexes. Curr Protoc Mol Biol. 2003 Aug; Chapter 27:Unit 27.1.
    View in: PubMed
  27. Luo MJ, Reed R. Identification of RNA binding proteins by UV cross-linking. Curr Protoc Mol Biol. 2003 Aug; Chapter 27:Unit 27.2.
    View in: PubMed
  28. Reed R. Coupling transcription, splicing and mRNA export. Curr Opin Cell Biol. 2003 Jun; 15(3):326-31.
    View in: PubMed
  29. Zhou Z, Licklider LJ, Gygi SP, Reed R. Comprehensive proteomic analysis of the human spliceosome. Nature. 2002 Sep 12; 419(6903):182-5.
    View in: PubMed
  30. Zhou Z, Sim J, Griffith J, Reed R. Purification and electron microscopic visualization of functional human spliceosomes. Proc Natl Acad Sci U S A. 2002 Sep 17; 99(19):12203-7.
    View in: PubMed
  31. Chung S, Zhou Z, Huddleston KA, Harrison DA, Reed R, Coleman TA, Rymond BC. Crooked neck is a component of the human spliceosome and implicated in the splicing process. Biochim Biophys Acta. 2002 Jul 19; 1576(3):287-97.
    View in: PubMed
  32. Strässer K, Masuda S, Mason P, Pfannstiel J, Oppizzi M, Rodriguez-Navarro S, Rondón AG, Aguilera A, Struhl K, Reed R, Hurt E. TREX is a conserved complex coupling transcription with messenger RNA export. Nature. 2002 May 16; 417(6886):304-8.
    View in: PubMed
  33. Maniatis T, Reed R. An extensive network of coupling among gene expression machines. Nature. 2002 Apr 4; 416(6880):499-506.
    View in: PubMed
  34. Reed R, Hurt E. A conserved mRNA export machinery coupled to pre-mRNA splicing. Cell. 2002 Feb 22; 108(4):523-31.
    View in: PubMed
  35. Luo ML, Zhou Z, Magni K, Christoforides C, Rappsilber J, Mann M, Reed R. Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly. Nature. 2001 Oct 11; 413(6856):644-7.
    View in: PubMed
  36. Reed R, Magni K. A new view of mRNA export: separating the wheat from the chaff. Nat Cell Biol. 2001 Sep; 3(9):E201-4.
    View in: PubMed
  37. Chua K, Reed R. An upstream AG determines whether a downstream AG is selected during catalytic step II of splicing. Mol Cell Biol. 2001 Mar; 21(5):1509-14.
    View in: PubMed
  38. Clouse KN, Luo MJ, Zhou Z, Reed R. A Ran-independent pathway for export of spliced mRNA. Nat Cell Biol. 2001 Jan; 3(1):97-9.
    View in: PubMed
  39. Zhou Z, Luo MJ, Straesser K, Katahira J, Hurt E, Reed R. The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans. Nature. 2000 Sep 21; 407(6802):401-5.
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  40. DeGiorgio CM, Schachter SC, Handforth A, Salinsky M, Thompson J, Uthman B, Reed R, Collins S, Tecoma E, Morris GL, Vaughn B, Naritoku DK, Henry T, Labar D, Gilmartin R, Labiner D, Osorio I, Ristanovic R, Jones J, Murphy J, Ney G, Wheless J, Lewis P, Heck C. Prospective long-term study of vagus nerve stimulation for the treatment of refractory seizures. Epilepsia. 2000 Sep; 41(9):1195-200.
    View in: PubMed
  41. Reed R. Mechanisms of fidelity in pre-mRNA splicing. Curr Opin Cell Biol. 2000 Jun; 12(3):340-5.
    View in: PubMed
  42. Das R, Zhou Z, Reed R. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell. 2000 May; 5(5):779-87.
    View in: PubMed
  43. Luo MJ, Reed R. Splicing is required for rapid and efficient mRNA export in metazoans. Proc Natl Acad Sci U S A. 1999 Dec 21; 96(26):14937-42.
    View in: PubMed
  44. Chua K, Reed R. The RNA splicing factor hSlu7 is required for correct 3' splice-site choice. Nature. 1999 Nov 11; 402(6758):207-10.
    View in: PubMed
  45. Das R, Reed R. Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels. RNA. 1999 Nov; 5(11):1504-8.
    View in: PubMed
  46. Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R. Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155. Mol Cell Biol. 1999 Oct; 19(10):6796-802.
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  47. Will CL, Schneider C, Reed R, Lührmann R. Identification of both shared and distinct proteins in the major and minor spliceosomes. Science. 1999 Jun 18; 284(5422):2003-5.
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  48. Reed R, Chiara MD. Identification of RNA-protein contacts within functional ribonucleoprotein complexes by RNA site-specific labeling and UV crosslinking. Methods. 1999 May; 18(1):3-12.
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  49. Chua K, Reed R. Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing. Genes Dev. 1999 Apr 1; 13(7):841-50.
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  50. Bedford MT, Reed R, Leder P. WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif. Proc Natl Acad Sci U S A. 1998 Sep 1; 95(18):10602-7.
    View in: PubMed
  51. Seghezzi W, Chua K, Shanahan F, Gozani O, Reed R, Lees E. Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells. Mol Cell Biol. 1998 Aug; 18(8):4526-36.
    View in: PubMed
  52. Gozani O, Potashkin J, Reed R. A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site. Mol Cell Biol. 1998 Aug; 18(8):4752-60.
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  53. Wang C, Chua K, Seghezzi W, Lees E, Gozani O, Reed R. Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis. Genes Dev. 1998 May 15; 12(10):1409-14.
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  54. Zhou Z, Reed R. Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing. EMBO J. 1998 Apr 1; 17(7):2095-106.
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  55. Chiara MD, Palandjian L, Feld Kramer R, Reed R. Evidence that U5 snRNP recognizes the 3' splice site for catalytic step II in mammals. EMBO J. 1997 Aug 1; 16(15):4746-59.
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  56. Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 1997 May 30; 89(5):781-7.
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  57. Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R. Association of U2 snRNP with the spliceosomal complex E. Nucleic Acids Res. 1997 Jan 15; 25(2):354-61.
    View in: PubMed
  58. Chiara MD, Gozani O, Bennett M, Champion-Arnaud P, Palandjian L, Reed R. Identification of proteins that interact with exon sequences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly. Mol Cell Biol. 1996 Jul; 16(7):3317-26.
    View in: PubMed
  59. Reed R. Initial splice-site recognition and pairing during pre-mRNA splicing. Curr Opin Genet Dev. 1996 Apr; 6(2):215-20.
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  60. Gozani O, Feld R, Reed R. Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A. Genes Dev. 1996 Jan 15; 10(2):233-43.
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  61. Staknis D, Reed R. Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes. Nucleic Acids Res. 1995 Oct 25; 23(20):4081-6.
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  62. Champion-Arnaud P, Gozani O, Palandjian L, Reed R. Accumulation of a novel spliceosomal complex on pre-mRNAs containing branch site mutations. Mol Cell Biol. 1995 Oct; 15(10):5750-6.
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  63. Chiara MD, Reed R. A two-step mechanism for 5' and 3' splice-site pairing. Nature. 1995 Jun 8; 375(6531):510-3.
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  64. Staknis D, Reed R. SR proteins promote the first specific recognition of Pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex. Mol Cell Biol. 1994 Nov; 14(11):7670-82.
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  65. Champion-Arnaud P, Reed R. The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes Dev. 1994 Aug 15; 8(16):1974-83.
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  66. Gozani O, Patton JG, Reed R. A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction. EMBO J. 1994 Jul 15; 13(14):3356-67.
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  67. Chiara MD, Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R. Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114. Proc Natl Acad Sci U S A. 1994 Jul 5; 91(14):6403-7.
    View in: PubMed
  68. Staknis D, Reed R. Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly. Mol Cell Biol. 1994 May; 14(5):2994-3005.
    View in: PubMed
  69. Bennett M, Reed R. Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science. 1993 Oct 1; 262(5130):105-8.
    View in: PubMed
  70. Michaud S, Reed R. A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals. Genes Dev. 1993 Jun; 7(6):1008-20.
    View in: PubMed
  71. Bennett M, Michaud S, Kingston J, Reed R. Protein components specifically associated with prespliceosome and spliceosome complexes. Genes Dev. 1992 Oct; 6(10):1986-2000.
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  72. Bennett M, Piñol-Roma S, Staknis D, Dreyfuss G, Reed R. Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro. Mol Cell Biol. 1992 Jul; 12(7):3165-75.
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  73. Michaud S, Reed R. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes Dev. 1991 Dec; 5(12B):2534-46.
    View in: PubMed
  74. Reed R. Protein composition of mammalian spliceosomes assembled in vitro. Proc Natl Acad Sci U S A. 1990 Oct; 87(20):8031-5.
    View in: PubMed
  75. Reed R. The organization of 3' splice-site sequences in mammalian introns. Genes Dev. 1989 Dec; 3(12B):2113-23.
    View in: PubMed
  76. Berkey CS, Reed RB. A model for describing normal and abnormal growth in early childhood. Hum Biol. 1987 Dec; 59(6):973-87.
    View in: PubMed
  77. Cormier HJ, Borus JF, Reed RB, Pinard G, Lessard R. [Identifying mental health service needs of persons with schizophrenia]. Can J Psychiatry. 1987 Aug; 32(6):454-8.
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  78. Phillips ME, Margolis RJ, Merot Y, Sober AJ, Reed RJ, Muhlbauer JE, Mihm MC. The spectrum of minimal deviation melanoma: a clinicopathologic study of 21 cases. Hum Pathol. 1986 Aug; 17(8):796-806.
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  79. Muhlbauer JE, Margolis RJ, Mihm MC, Reed RJ. Minimal deviation melanoma: a histologic variant of cutaneous malignant melanoma in its vertical growth phase. J Invest Dermatol. 1983 Jun; 80(1 Suppl):63s-5s.
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  80. Dwyer JT, Andrew EM, Berkey C, Valadian I, Reed RB. Growth in "new" vegetarian preschool children using the Jenss-Bayley curve fitting technique. Am J Clin Nutr. 1983 May; 37(5):815-27.
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  81. Berkey CS, Reed RB, Valadian I. Midgrowth spurt in height of Boston children. Ann Hum Biol. 1983 Jan-Feb; 10(1):25-30.
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  82. Berkey CS, Reed RB, Valadian I. Longitudinal growth standards for preschool children. Ann Hum Biol. 1983 Jan-Feb; 10(1):57-67.
    View in: PubMed
  83. Cronk CE, Roche AF, Kent R, Berkey C, Reed RB, Valadian I, Eichorn D, McCammon R. Longitudinal trends and continuity in weight/stature from 3 months to 18 years. Hum Biol. 1982 Dec; 54(4):729-49.
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  84. Waber DP, Vuori-Christiansen L, Ortiz N, Clement JR, Christiansen NE, Mora JO, Reed RB, Herrera MG. Nutritional supplementation, maternal education, and cognitive development of infants at risk of malnutrition. Am J Clin Nutr. 1981 Apr; 34(Suppl 4):807-13.
    View in: PubMed
  85. Valadian I, Berkey C, Reed RB. Adolescent nutrition as it relates to cardiovascular disease and reproductive capacity later in life. Nutr Rev. 1981 Feb; 39(2):107-11.
    View in: PubMed
  86. Needleman HL, Gunnoe C, Leviton A, Reed R, Peresie H, Maher C, Barrett P. Deficits in psychologic and classroom performance of children with elevated dentine lead levels. N Engl J Med. 1979 Mar 29; 300(13):689-95.
    View in: PubMed
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